Analytica Chimica Acta最新文献

{"title":"Matrix solid-phase dispersion, combined with online SPE-LC-MS/MS, for the determination of tetracyclines and their main transformation products in sludge and agricultural soils","authors":"Noelia García-Criado,&nbsp;Julia Martín,&nbsp;Juan Luis Santos,&nbsp;Irene Aparicio,&nbsp;Esteban Alonso","doi":"10.1016/j.aca.2025.344749","DOIUrl":"10.1016/j.aca.2025.344749","url":null,"abstract":"<div><h3>Background</h3><div>Tetracyclines are frequently detected in the environment due to their persistence and extensive use as antibiotics in human and veterinary medicine. They can accumulate in sludge and in agricultural soils fertilised with treated sludge or manure. This behaviour may lead to ecological risk, propagation of antibiotic resistance and can affect crops and human health through the consumption of vegetables and fruits. Analytical methods for the determination of tetracyclines and their transformation products (TPs) in sludge and agricultural soils are scarce, include a small number of tetracyclines, do not include their TPs and are not specifically developed for their determination.</div></div><div><h3>Results</h3><div>A matrix solid-phase dispersion (MSPD) method has been optimised for the simultaneous determination of six tetracyclines and six of their main TPs in the types of sludge applied as soil fertiliser (digested sludge and composted sludge) and in agricultural soil. Analytical determination by online SPE-LC-MS/MS has also been optimised. The method was validated for each of the above-mentioned matrices, with good linearity (R² ≥ 0.98), precision (RSD ≤12.3 %), accuracy (64–117 %) and method quantification limits (below 10 ng g⁻¹ dry weight (dw) for most compounds). Application of the method to digested sludge, composted sludge and agricultural soils revealed high concentrations not only of the parent compounds (tetracycline and doxycycline, up to 353 ng g⁻¹ dw and 1069 ng g⁻¹ dw, respectively, in digested sludge) but also of their TPs (epitetracycline, anhydrotetracycline and 4-epioxytetracycline up to 327 ng g⁻¹ dw, 117 ng g⁻¹ dw and 10.2 ng g⁻¹ dw, respectively, in digested sludge).</div></div><div><h3>Significance and novelty</h3><div>This method allows the specific simultaneous determination of six tetracyclines and six of their TPs in sludge and soils. The proposed method constitutes a starting point to extend the use of MSPD, that is being applied mainly to biota and food, as a low-cost and easy-to-perform technique that allows simultaneous extraction and clean-up. Application of the method revealed the significance of the determination of TPs of tetracyclines for a proper environmental risk assessment.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1379 ","pages":"Article 344749"},"PeriodicalIF":6.0,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoscale morphological and spectroscopic mapping of zinc carboxylate formation in oil and tempera paintings","authors":"Chiaramaria Stani ,&nbsp;Giorgia Sciutto ,&nbsp;Giovanni Birarda ,&nbsp;Emilio Catelli ,&nbsp;Lisa Vaccari ,&nbsp;Silvia Prati","doi":"10.1016/j.aca.2025.344740","DOIUrl":"10.1016/j.aca.2025.344740","url":null,"abstract":"<div><h3>Background</h3><div>This study explores the application of infrared scattering-type Scanning Near-field Optical Microscopy (IR s-SNOM) to analyze zinc white paint models at the nanoscale, focusing on the formation and crystallization of zinc carboxylates. Zinc carboxylates are critical degradation products in oil and tempera paintings, causing brittleness, delamination, and color changes. Our research applies IR s-SNOM to paint samples prepared with oil and egg binders, subjected to both natural and artificial aging.</div></div><div><h3>Results</h3><div>The impact of environmental conditions and binding mediums on inter-sample variability was highlighted using a chemometric approach. Notably, zinc carboxylates form more rapidly in oil binders under artificial aging, while natural ageing results in more ordered crystalline structures in egg binders. While the analysis of individual IR s-SNOM spectra did not reveal an unambiguous correlation between morphology and chemistry at the nanoscale, it allowed to disentangle the causes of intra-sample variability. The surface sensitivity guaranteed by IR s-SNOM was the key element for disclosing spectral features hidden by micro-approaches, such as the identification of the broad band in the 1700-1500 cm⁻¹ spectral region in the egg medium, which had never been reported before.</div></div><div><h3>Significance</h3><div>This study highlights the critical role that nanoscale analysis may play in advancing the understanding of degradation mechanisms, offering valuable insights for art conservation. By transitioning from average, bulk analysis to nanoscale investigations, IR s-SNOM proved to be a powerful tool for developing targeted conservation strategies, ultimately enhancing the preservation of cultural heritage.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1380 ","pages":"Article 344740"},"PeriodicalIF":6.0,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate construction of cell membrane biomimetic magnetic fluorescent carbon nanoplatform via layer-by-layer assembly for targeted capture of drug leads","authors":"Yi Qin ,&nbsp;Xu Jiang ,&nbsp;Heng Ye ,&nbsp;Runuo Wang ,&nbsp;Yang Liu ,&nbsp;Xuefeng Guan ,&nbsp;Longshan Zhao","doi":"10.1016/j.aca.2025.344741","DOIUrl":"10.1016/j.aca.2025.344741","url":null,"abstract":"<div><h3>Background</h3><div>As an effective source of novel drug discovery, natural products have important contributions in the treatment of various diseases. Pathological cell membrane biomimetic modified magnetic carbon nano-platform shows great potential in targeted screening of bioactive ingredients. Meanwhile, the introduction of cellular imaging technology enables the platform to achieve important advantages in real-time identification of pharmacological activities of active ingredients.</div></div><div><h3>Results</h3><div>Breast cancer cell membrane biomimetic composites (CMMFCNTs) were accurately constructed via layer-by-layer assembly and utilized as a drug discovery platform to screen potential anti-cancer ingredients from <em>Taraxacum mongolicum</em>. Comprehensive characterization techniques indicated that the synthesized CMMFCNTs possessed excellent magnetic properties (saturation magnetization: 42.51 emu·g⁻¹), high adsorption capacity (17.53 μg mg⁻¹), and were well-characterized by spectroscopic and morphological techniques. Finally, four potential anti-cancer components: phlorizin, loganin, diosmetin, and hexahydrocurcumin were accurately captured. Molecular docking demonstrated strong binding between these compounds and cell membrane receptors (P-gp, HER2, ERα), supported by favorable binding energies (range: −7.5 to −9.1 kcal∙mol⁻¹). Moreover, their inhibitory effects on the proliferation of breast cancer cells were preliminarily verified by cell imaging experiments and wound scratch assay.</div></div><div><h3>Significance</h3><div>Although various applications of cell membrane biomimetic screening have been reported in previous studies, cell imaging technology has been used less frequently in it. In addition, this study also explored the inter-binding effect of the screened active ingredients with breast cancer cell membrane-associated receptors to further validate the potential pharmacological effects of the screened active ingredients. In conclusion, magnetic fluorescent carbon nanoplatforms based on pathological cell membrane mimicry show great promise for active ingredients screening in complex matrices, facilitating natural product-based development.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1379 ","pages":"Article 344741"},"PeriodicalIF":6.0,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-functional carbon dots for fluorescent detection of L-Glutamic acid and 2-Methylimidazole in complex matrices: Applications in food safety and environmental monitoring","authors":"Yalin Ju,&nbsp;Min Zheng","doi":"10.1016/j.aca.2025.344742","DOIUrl":"10.1016/j.aca.2025.344742","url":null,"abstract":"<div><h3>Background</h3><div>L-Glutamic acid (L-Glu) serves as both an umami substance in foods and a key neurotransmitter, yet abnormal levels may cause metabolic disorders and neurological disorders. 2-Methylimidazole (2-MI), a synthetic compound used in metal-organic frameworks (MOFs), pesticides, and dyes, is formed during caramel color production. It exhibits toxicity and carcinogenicity and poses environmental risks due to industrial discharge. However, conventional detection methods are limited by their reliance on costly instrumentation and specialized personnel. Therefore, there is an urgent need to develop a fluorescent probe for detecting L-Glu and 2-MI that is easy to operate, rapid in response, and highly sensitive.</div></div><div><h3>Results</h3><div>Using a solvothermal technique, carbon dots (CDs) were fabricated from alkaline lignin and resazurin and were characterized as spherical nanoparticles (11.0 ± 3.4 nm) with graphitic structure and oxygen/nitrogen/sulfur-containing surface groups. These CDs exhibited excitation-independent orange emission (λex/λem = 571/582 nm), ionic stability, and reversible pH sensitivity (pKa = 4.54). Capitalizing on their selective fluorescence response, CDs served as a dual-functional probe: L-Glutamic acid (L-Glu) selectively quenched CDs fluorescence via static quenching (LOD = 0.17 μM, linear range 0–20 μM), while 2-Methylimidazole (2-MI) restored fluorescence in the CDs/L-Glu system (LOD = 0.39/0.14 μM, linear range 0–50/50–100 μM).</div></div><div><h3>Significance</h3><div>The probe demonstrated exceptional anti-interference capability against coexisting ions/analytes and operational simplicity. Validated in actual world matrices, standard addition recoveries ranged from 87.44 to 111.38 % for L-Glu in kelp soup/purified drinking water and 93.23–107.01 % for 2-MI in snowmelt/purified drinking water. This work establishes CDs as a robust platform for the rapid detection of food safety and environmental contaminants.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1379 ","pages":"Article 344742"},"PeriodicalIF":6.0,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photocatalytic proximity labeling coupled with cross-linking for deciphering of the lysosome proteome in living cells","authors":"He Wang ,&nbsp;Zichun Qiao ,&nbsp;Lihua Zhang ,&nbsp;Bo Jiang ,&nbsp;Yukui Zhang","doi":"10.1016/j.aca.2025.344737","DOIUrl":"10.1016/j.aca.2025.344737","url":null,"abstract":"<div><h3>Background</h3><div>Lysosomes are essential organelles that play vital roles in the degradation and recycling of a variety of biomolecules. To fully understand their molecular mechanisms, high spatiotemporal identification of the lysosome proteome in living cells is critical. Although photocatalytic proximity labeling is a powerful tool for profiling subcellular proteome, it often struggles with low labeling efficiency, particularly for low-abundance proteins.</div></div><div><h3>Results</h3><div>To overcome this, we introduced a novel strategy that integrates photocatalytic proximity labeling with cross-linking. The lysosome-targeting photosensitizer (MP-AcDBF) catalyzed the covalent reaction between proximal proteins and enrichable nucleophilic substrate within lysosome based on singlet oxygen. Then the cross-linker allowed difficult-to-label proteins (low-abundance or transiently localized proteins) to be linked to pre-labeled ones, thereby expanding the scope of protein identification while preserving lysosomal specificity via enrichment of labeled handles. Using the cross-linking-assisted photocatalytic proximity labeling (CLAPL) strategy, we successfully identified 238 lysosome-annotated proteins in living HeLa cells, covering luminal, transmembrane and membrane-associated proteins, enabling a relative increase compared to method without cross-linking (238 vs 197). Additionally, CLAPL was further applied to map dynamic lysosomal proteome and revealed stress-induced metabolic alterations.</div></div><div><h3>Significance</h3><div>The developed strategy enhanced lysosomal proteomic coverage via a high-precision integration platform, allowing systematic identification of both membrane-associated and luminal proteins within lysosome, thereby providing mechanistic insights into subcellular biology and unlocking new frontiers in cellular research.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1379 ","pages":"Article 344737"},"PeriodicalIF":6.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multimodal characterization methodology of non-metallic inclusions in metal materials using laser-induced breakdown spectroscopy precision-targeted analysis","authors":"Xiaofen Zhang ,&nbsp;Yunhai Jia ,&nbsp;Liang Sheng ,&nbsp;Danyang Zhi ,&nbsp;Wenyu Zhang ,&nbsp;Lei Yu ,&nbsp;Miao He ,&nbsp;Peifeng Cheng","doi":"10.1016/j.aca.2025.344738","DOIUrl":"10.1016/j.aca.2025.344738","url":null,"abstract":"<div><h3>Background</h3><div>The determination of non-metallic inclusion with varying chemical compositions and microstructure in metal materials is helpful for improvement and development of internal quality and property. The recently proposed laser-induced breakdown spectroscopy (LIBS) method can rapidly identify simple diatomic inclusions and determine inclusion equivalent diameter. However, due to the lack of steel-matrix inclusion standard samples, the LIBS scanning measurements typically combined with scanning electron microscopy coupled with energy dispersive spectrometry (SEM-EDS) and statistical methods, which makes this method unable to conduct independent measurements.</div></div><div><h3>Results</h3><div>In this paper, we propose a LIBS precision-targeted analysis methodology for multimodal characterization of chemical composition and microstructure for inclusions. A three-dimensional high-precision motion platform system and a microscopic imaging system were developed and constructed on the LIBS detection platform to achieve precise targeted positioning and measurement. The experimental conditions, including the relative distance between the microscope and LIBS, and the excitation mode of inclusions, were systematically optimized to improve the spectral quality and detection limits of LIBS precision-targeted analysis. More importantly, for the identification and quantitative analysis of complex and varied inclusions composed of varying element contents, univariate steel-matrix standard samples with controlled inclusions were designed and prepared. The concentration-spectral intensity calibration curves for Mn, Al, Ca, Mg, Ti, and Si elements were established, and the results were validated by conducting SEM-EDS.</div></div><div><h3>Significance</h3><div>This new method overcomes shortcomings of LIBS undirected scanning, significantly improving analytical efficiency and throughput, while opening the way for the high-throughput characterization of non-metallic inclusions in large-sized samples. This technique provided an effective means for the complex inclusion composition identification and cleanness process evaluation of metal material samples and thus for research and improvement of material property.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1379 ","pages":"Article 344738"},"PeriodicalIF":6.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mode fluorescence/colorimetric aptasensor based on Zn,Ce-MOF nanocomposite for sensitive detection of isocarbophos","authors":"Yimeng Li ,&nbsp;Jiayao Wang ,&nbsp;Yi Ran ,&nbsp;Yiwei Liu ,&nbsp;Xiaopeng Hu","doi":"10.1016/j.aca.2025.344739","DOIUrl":"10.1016/j.aca.2025.344739","url":null,"abstract":"<div><h3>Background</h3><div>A dual-mode fluorescence/colorimetric aptasensor was developed for the sensitive detection of isocarbophos, an organophosphorus pesticide posing significant public health risks. Using Zn,Ce-MOF nanocomposites, this sensor integrated magnetic separation and signal amplification. This integrated biosensing strategy offers a promising tool for environmental safety assurance and food quality control applications.</div></div><div><h3>Results</h3><div>The Zn,Ce-MOF nanozyme of this sensor serves as both a carrier of 3,3′,5,5′-tetramethylbenzidine (TMB) and oxidase mimic, facilitating enzyme-free TMB oxidation. The dual-readout system enables self-validating detection through independent channels (fluorescence LOD: 0.06 ng mL⁻¹; colorimetric LOD: 0.09 ng mL⁻¹), with robust performance, high interferent selectivity, and reproducibility. Practical validation in spiked agricultural samples yielded recovery rates of 97.7–113.6 %, confirming the platform's suitability for field-deployable pesticide monitoring.</div></div><div><h3>Significance</h3><div>This work developed a fluorescence/visual aptasensor with self-assembled TMB/Au/Zn,Ce-MOF probes for one-step magnetic separation dual-mode detection. This integrated biosensing strategy improves detection efficiency and accuracy, offering a general strategy for multimodal biosensors.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1379 ","pages":"Article 344739"},"PeriodicalIF":6.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate and sensitive determination of sialic acid using three-dimensional Raman imaging based on a background-free SERS strategy","authors":"Huixia Di ,&nbsp;Zhouhao Lei ,&nbsp;Qianyu Li,&nbsp;Peize Xu,&nbsp;Yadi Wang","doi":"10.1016/j.aca.2025.344736","DOIUrl":"10.1016/j.aca.2025.344736","url":null,"abstract":"<div><h3>Background</h3><div>Sialic acid (SA) is a significant monosaccharide presented as the terminal moieties of glycoproteins or glycolipids on the cell membrane and closely correlates with various biological and pathological activities, especially in tumor growth and metastasis. It is well known that the overexpression of SA levels could facilitate tumor immune escape, cell proliferation, and angiogenesis. Thus, SA profiling is critical for the early identification and long-term monitoring of tumors. However, it remains limited by the sensitivity and accuracy of the currently available techniques.</div></div><div><h3>Results</h3><div>Herein, we proposed a three-dimensional (3D) surface-enhanced Raman scattering (SERS) imaging with a background-free pattern to determine the SA expression at the single-cell level based on a signal amplification strategy. In this study, the target cells were first captured by the phenylboronic acid (PBA) ligands-coated plasmonic gold (pAu) film and further conjugated with benzonitrile-encoded gold SERS nanotags (Au-MBN@Au@PBA) due to the borate bonds, resulting in an enhanced plasmonic structure to generate a wide variety of “hot spots” to improve the SERS signals at 2230 cm⁻¹ channel in the cellular Raman-silent region (1800–2800 cm⁻¹). In this study, the 3D SERS imaging enables a deep insight into SA levels, determination of the normal and cancer cell lines, and real-time monitoring of nanoparticle tracking in cancer cells.</div></div><div><h3>Significance</h3><div>In this work, the newly prepared PBA ligands possess various capabilities, including modification of SERS nanotags and pAu films, generating good affinity toward SA moieties, and increasing the hydrophilicity of pAu substrate for cell capture. With the assistance of PBA ligands, the pAu film-cell-SERS nanotags sandwich structure was yielded, and the enhanced plasmon coupling was produced, which allows noninvasive, high-precision, and sensitive SA profiling for early tumor diagnosis, glycosylation exploration, and tracking phagocytosis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1379 ","pages":"Article 344736"},"PeriodicalIF":6.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method validation and uncertainty evaluation in trace element analysis of high-purity silver by ICP-OES","authors":"Dinesh Singh ,&nbsp;V.N. Singh ,&nbsp;S.P. Singh","doi":"10.1016/j.aca.2025.344732","DOIUrl":"10.1016/j.aca.2025.344732","url":null,"abstract":"<div><div>Silver is a precious metal, and high-purity silver is widely used in electronic, optical, and reference material applications, where even trace amounts of impurities can critically impact performance and accuracy. Quantifying trace elements such as Cu, Pb, Fe, and others in high-purity silver poses analytical challenges due to the potential matrix effects and the need for accurate calibration strategies. The trace impurities, Cu, Fe, and Pb in high-purity silver samples were quantified using the standard addition (SAM) and the matrix-matched external standard method (MMESM) using inductively coupled plasma optical emission spectrometry (ICP-OES), and their results are compared. The research also includes a comprehensive uncertainty evaluation associated with each method. Validating parameters like LOD (Limit of Detection), LOQ (Limit of Quantification), working range, accuracy, and precision are also discussed in detail in the manuscript. The results obtained from both the calibration approaches were found to be comparable. Both methods were found to have the ability to account for the matrix effect. The recovery found for the results indicates that both methods provide reliable quantification. The two-way ANOVA results demonstrate that both emission lines and matrix concentrations yield statistically comparable results for copper, iron, and lead determination by SAM &amp; MMESM. Two extra emission lines for copper and one for iron estimation were observed and are reported here. Also, the results for both SAM and MMESM with and without internal standard correction were quantified, and the results were almost the same. Thus, this work provides a comprehensive understanding of trace element analysis in silver, which can also be extended to other metals for trace element analysis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1379 ","pages":"Article 344732"},"PeriodicalIF":6.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial atlasing of N-glycosylation in healthy control and clear cell renal cell carcinoma tissues linked with immune checkpoint inhibition response by MALDI mass spectrometry imaging","authors":"Caroline Kittrell ,&nbsp;Lyndsay E.A. Young ,&nbsp;Colin McDowell ,&nbsp;Grace Grimsley ,&nbsp;Anand S. Mehta ,&nbsp;Peggi M. Angel ,&nbsp;Jessica Schmitz ,&nbsp;Nikolaos Vasileiadis ,&nbsp;Christopher Darr ,&nbsp;Viktor Grünwald ,&nbsp;Philipp Ivanyi ,&nbsp;Jan Hinrich Bräsen ,&nbsp;Richard R. Drake","doi":"10.1016/j.aca.2025.344724","DOIUrl":"10.1016/j.aca.2025.344724","url":null,"abstract":"<div><h3>Background</h3><div>Clear cell renal cell carcinoma (ccRCC) is the most common subtype of kidney cancer. While most cases are detected early and treated by surgery, a relevant proportion of patients present with metastases and require medical treatment. Immune checkpoint inhibition (ICI) has become a key component of treatment for metastatic ccRCC patients and may produce complete responses in a fraction of patients. Presently, there is no predictive biomarker for response to ICI which could guide individualized treatments, enrich response rates and minimize unnecessary treatment in patients unlikely to benefit from ICI.</div></div><div><h3>Results</h3><div>In this study, we employed Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging to characterize the N-glycome of healthy control kidneys and 21 treatment naïve, primary RCC tumor samples. Patients were treated with ICI following tumor resection and stratified by response. Two candidate N-glycans were found to be upregulated in the group that experienced longer progression free survival and exhibited a response to PD-1/PD-L1 checkpoint inhibition. Additionally, we established N-glycosylation patterns which correspond to renal microanatomic structures including proximal convoluted tubules, distal convoluted tubules, and glomeruli. To more fully evaluate the tumor microenvironment of the different immunotherapy responder groups, we also conducted innovative immunohistochemical analyses visualized via mass spectrometry.</div></div><div><h3>Significance</h3><div>This study represents an expanded spatial characterization of the normal renal N-glycome. Additionally, the N-glycomes of ccRCC tissues were compared relative to normal tissues, and in context of response to ICI. Candidate glycan-based biomarkers could eventually be developed for clinical translational applications at baseline to predictively identify responders and non-responders to ICI.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1380 ","pages":"Article 344724"},"PeriodicalIF":6.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145203415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}