Analytica Chimica Acta最新文献

{"title":"Novel diffusion gradient in thin-films based on antibiofouling Ag/polydopamine membranes for measurement of antibiotics in natural waters","authors":"Jiaxing Xie ,&nbsp;Fan Ge ,&nbsp;Xinyi Yan ,&nbsp;Suyu Ren ,&nbsp;Yan Wang ,&nbsp;Feng Tan","doi":"10.1016/j.aca.2026.345157","DOIUrl":"10.1016/j.aca.2026.345157","url":null,"abstract":"<div><h3>Background</h3><div>The diffusion gradient in thin-films (DGT) device is widely used for in situ measurement of pollutants in aquatic environments, offering high enrichment efficiency and robustness to environmental variations such as temperature and hydrodynamics. These characteristics make DGT particularly suitable for monitoring a diverse array of trace organic contaminants in waters. However, long-term deployment is often compromised by biofilm formation on the sampling outer membrane, which can obstruct analyte diffusion and lead to underestimation of analyte concentrations. This study addresses the need for a biofouling-resistant DGT device that maintains accuracy during extended field applications.</div></div><div><h3>Results</h3><div>A novel DGT device incorporating an antifouling Ag/polydopamine-modified polyethersulfone (Ag/PDA-PES) membrane was developed. The modified membrane demonstrated dual functional improvements: a 40 % reduction in water contact angle, indicating enhanced hydrophilicity, and strong antimicrobial efficacy, evidenced by an inhibition zone of 1165 μm ± 110 μm against <em>Escherichia coli</em>, while maintaining diffusion characteristics comparable to the original PES membrane. In extended deployment in both simulated and natural waters, the Ag/PDA-PES membrane effectively inhibited biofilm formation and maintained stable analyte diffusion coefficients over time compared to the unmodified membrane. The DGT devices showed constant sampling performance across varying pH (5.0–9.0), ionic strength (0–200 mM NaCl), and dissolved organic matter levels (0–20 mg/L). Field validation confirmed the device reliability, with measured total antibiotic concentrations (692–1062 ng/L) consistent with grab sampling.</div></div><div><h3>Significance</h3><div>This work signifies a step forward in DGT passive sampling technology by integrating antifouling properties directly into the membrane, thereby enabling accurate long-term monitoring of pollutants in biofilm-prone waters. Moreover, this study provides a promising strategy for improving the reliability of long-term DGT sampling, with meaningful implications for the precise monitoring and ecological risk assessment of other emerging pollutants.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1393 ","pages":"Article 345157"},"PeriodicalIF":6.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146089623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A deprotection-oxidation cascade-regulated dual-responsive near-infrared fluorescent probe for individual and sequential detection of H2S/HClO with applications in food safety monitoring","authors":"Jie Yang ,&nbsp;Chao Fu ,&nbsp;Yicong Zhou ,&nbsp;Hua Zhang ,&nbsp;Xiangzhi Song ,&nbsp;Dandan Li ,&nbsp;Chuanxiang Liu","doi":"10.1016/j.aca.2026.345191","DOIUrl":"10.1016/j.aca.2026.345191","url":null,"abstract":"<div><h3>Background</h3><div>Hydrogen sulfide (H₂S) and hypochlorous acid (HClO), as critical reactive sulfur (RSS) and oxygen (ROS) species, respectively play dual roles in physiological regulation and food safety. While numerous fluorescent probes exist for individual detection of H₂S or HClO, dual-functional probes remain rare despite their significant potential in food safety, environmental monitoring, and biomedicine.</div></div><div><h3>Results</h3><div>A novel A-π-A (acceptor-π-bridge-acceptor) type near-infrared fluorescent probe, <strong>DCIQ-DNBS</strong>, was developed for the first time, featuring dual-response mechanisms for individual and sequential detection of H₂S and HClO via H₂S-triggered thiolysis of the 2,4-dinitrobenzenesulfonyl (DNBS) and HClO mediated DNBS<strong>-</strong>deprotection-oxidation of electron-deficient C<img>C bond cascade oxidation. The probe <strong>DCIQ-DNBS</strong> demonstrates exceptional selectivity and rapid response times, and offers dual-channel fluorescence signaling with non-overlapping emissions: a near-infrared response at 740 nm for H₂S (LOD, 49 nM) and a short-wavelength yellow emission at 562 nm for HClO (LOD, 40 nM). Practical applications were demonstrated through <strong>DCIQ-DNBS</strong> loaded portable test strips, which successfully enabled visual monitoring of gaseous H₂S in food spoilage samples and quantitative tracing of HClO in foods. Furthermore, cellular studies revealed the probe's capability to detect elevated levels of both H₂S and HClO in HeLa cells.</div></div><div><h3>Significance and novelty</h3><div>This novel single-probe system enables the individual and sequential detection of two distinct analytes in a single assay. By generating unique emission signals for each target, it eliminates the need for separate tests or complex separation steps, thereby revolutionizing efficiency in fields like food safety monitoring.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1393 ","pages":"Article 345191"},"PeriodicalIF":6.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring lactoferrin as an innovative covalently immobilized chiral selector for the selective separation of pharmaceutical enantiomers using HPLC","authors":"Adel Ehab Ibrahim ,&nbsp;Samy G. Alamir ,&nbsp;Ghanem Al-Thani ,&nbsp;Sami El Deeb ,&nbsp;Ahmed Al-Harrasi","doi":"10.1016/j.aca.2026.345205","DOIUrl":"10.1016/j.aca.2026.345205","url":null,"abstract":"<div><h3>Background</h3><div>Molecular chirality has a profound impact on pharmaceutical drugs' efficacy and safety. Thus, chiral recognition plays a vital role in drug development throughout all process stages. LF is a globular glycoprotein composed of approximately 700 amino acids, that's capable of providing multiple interactions through its amino acid residues.</div></div><div><h3>Results</h3><div>In the proposed research, lactoferrin (LF) was covalently immobilized for the first time using a monolithic epoxy stationary phase via a Schiff's base formation as chiral selector (CS). LF-CS was evaluated for the enantioselective HPLC separation of 18 racemic pharmaceutical drugs. The chromatographic conditions were optimized for optimum enantio-separation. LF-CS proved its potential as a CS enabling the enantioseparation of 10 drug racemates: cetirizine, omeprazole, lansoprazole, dapoxetine, doxazosin, nebivolol, atenolol, bisoprolol, chlorthalidone and ofloxacin. Moreover, <em>in-silico</em> molecular docking studies were conducted to help understand the separation modes. LF as a CS reported hydrogen bonding, hydrophobic interactions, π bonding, van der Waals forces, and electrostatic interactions. Finally, the novel LF-CS was applied to determine the enantiomeric purity of marketed single-enantiomer pharmaceutical products, demonstrating its ability to verify their composition and identify impurities.</div></div><div><h3>Significance</h3><div>HPLC remains the primary choice for all pharmaceutical research, as it offers higher sensitivity, reliability, and reproducibility. This work introduces LF as a novel, multifunctional CS for HPLC, covalently immobilized for the first time, expanding the toolbox of protein-based chiral stationary phases. Moreover, the study also offers a critical insight into the limitations of relying solely on computational predictions, empirically demonstrating that solvent effects can override binding affinities, a phenomenon not captured by standard docking simulations.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1393 ","pages":"Article 345205"},"PeriodicalIF":6.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"·ATP-enhanced PB@MIL-100(Fe) nanozyme enables dual-mode ratiometric colorimetric and smartphone-based hydrogel detection of nitrite in food samples","authors":"Biyao Dai ,&nbsp;Quanyong Dong ,&nbsp;Taiqi Hao ,&nbsp;Shenghui Zhang ,&nbsp;Dong Xu ,&nbsp;Ying Xiong ,&nbsp;Wang Li ,&nbsp;Qian Wen ,&nbsp;Zhimei Huang ,&nbsp;Jiali Ren","doi":"10.1016/j.aca.2026.345192","DOIUrl":"10.1016/j.aca.2026.345192","url":null,"abstract":"<div><h3>Background</h3><div>Nitrite residues pose significant threats to food safety, as excessive consumption of this may cause serious health risks to human. Conventional techniques such as Griess reagent and chromatographic methods can successfully quantify nitrite levels, but these approaches often suffer from complex sample preparation, poor selectivity, expensive instrumentation, necessitate professional operators and time-consuming procedures. These limitations restrict their practical application for detection nitrites in point-of-care testing (POCT). Therefore, developing sensitive and portable nitrite detection methods remains critically important for food safety monitoring.</div></div><div><h3>Results</h3><div>Herein, we developed a novel dual-mode sensing platform based on composite nanozymes PB@MIL-100(Fe)@ATP for sensitive, selective and portable nitrite detection. This platform integrates Prussian Blue with MIL-100(Fe) metal-organic framework and exploits adenosine triphosphate (ATP) as an enhancer. The resulting PB@MIL-100(Fe)@ATP demonstrated excellent peroxidase-like (POD-like) catalytic activity, which catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to form blue-colored oxidized TMB (TMBox) with a characteristic absorption peak at 652 nm. In the presence of nitrites, TMBox specifically undergoes diazotization under acidic conditions to produce yellow diazotized TMB, exhibiting increased absorbance at 450 nm while decreasing at 652 nm. The ratiometric colorimetric assay achieved a detection limit of 0.17 μM within 20 min through absorbance ratio analysis at 652/450 nm. Furthermore, a smartphone-assisted hydrogel sensing method with detection limit of 1.68 μM was developed for point-of-care nitrites monitoring through image capture and color analysis, providing equipment-free operation.</div></div><div><h3>Significance</h3><div>The ATP-enhanced PB@MIL-100(Fe) nanozyme was successfully applied to detect nitrites with high recovery rates in real pickled vegetables, sauerkraut, and sausage samples. This dual-mode platform demonstrated excellent selectivity, high quantitative accuracy, and practical applicability in complex food matrices, providing a simple, rapid and versatile tool for on-site food safety monitoring.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1393 ","pages":"Article 345192"},"PeriodicalIF":6.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146116200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining laser-induced breakdown spectroscopy (LIBS) and time-gated Raman spectroscopy for underwater ores identification","authors":"Jiaojian Song ,&nbsp;Ye Tian ,&nbsp;Yuanyuan Xue ,&nbsp;Qingxi Liu ,&nbsp;Pingsai Chu ,&nbsp;Jinjia Guo ,&nbsp;Yuan Lu ,&nbsp;Ronger Zheng","doi":"10.1016/j.aca.2026.345204","DOIUrl":"10.1016/j.aca.2026.345204","url":null,"abstract":"<div><h3>Background</h3><div>As the land-based mineral deposits are depleting fast, deep-sea metal mineral resources have been proven to have a great mining value. Traditional deep-sea mineral detection techniques require ore samples to be brought back to the laboratory by geological sampling methods for chemical analysis. This approach suffers from substantial time delays and cannot provide real-time, large-scale and rapid feedback during the mining process. Therefore, there is a critical demand for the rapid and in-situ technique of underwater ores identification without requiring the ore samples to be brought back to the laboratory.</div></div><div><h3>Results</h3><div>We combined LIBS and time-gated Raman spectroscopy for the rapid identification of underwater ores. An integrated LIBS-Raman setup was built based on a same laser and a same spectrometer, to acquire both the elemental and molecular information of ore samples immersed in deionized water. PLS-DA was used to establish the classification model based on the LIBS and Raman spectra. Two data fusion strategies including data-level fusion and feature-level fusion were used for the combination of LIBS and Raman data. The data fusion strategies offer a significant improvement in ores classification compared to using the individual LIBS or Raman data. The data-level fusion model provides the best classification accuracy of 99.11%, while the feature-level fusion model has a slightly lower accuracy of 98.44% but with a much reduced computation time. The feature selection by successive projections algorithm (SPA) involved in the feature-level fusion improves the computing efficiency and the interpretability of the model.</div></div><div><h3>Significance</h3><div>The present results demonstrated the capability of LIBS and Raman techniques for the rapid identification of underwater ores. The integrated setup that combining LIBS with time-gated Raman spectroscopy based on a same laser source and a same spectrometer could be beneficial for developing down-sizing and low-power consumed underwater devices for the future deep-sea mining applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1393 ","pages":"Article 345204"},"PeriodicalIF":6.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146129703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering a Highly Selective FRET-Based Genetically Encoded Zn2+ Sensor for Real-Time Live-Cell Zinc Imaging","authors":"Chunhong Liu ,&nbsp;Yu Feng ,&nbsp;Jiang Zhu ,&nbsp;Li Jin ,&nbsp;Qingyuan Hu ,&nbsp;Wang Jue ,&nbsp;Hao Chen","doi":"10.1016/j.aca.2026.345470","DOIUrl":"10.1016/j.aca.2026.345470","url":null,"abstract":"<div><div>Genetically encoded Zn²⁺ probes (GEZPs) provide powerful tools for visualizing intracellular zinc dynamics with spatial and temporal resolution in living cells. Recently developed single-module ZnM-based scaffolds are derived from CaM-based metal-binding motifs and can serve as effective sensing elements. ZnM preferentially binds Zn²⁺, but their selectivity can be partially compromised by competing ions such as Ni²⁺, which share similar coordination properties. Here, we developed TV-ZnM1, a ratiometric FRET-based GEZP constructed through a stepwise engineering strategy centered on the ZnM sensing module. First, we optimized the linker architecture guided by AlphaFold3 structural predictions to improve donor–acceptor coupling. Next, we refined the metal-binding site through targeted residue substitutions, achieving high Zn²⁺ selectivity and reduced interference from competing divalent ions. The probe exhibits submicromolar Zn²⁺ affinity (Kd = 4.63 × 10^-7 M by fluorescence titration and 1.17 × 10^-7 M by PAR assay) and a robust ratiometric response with a 1.71-fold change in living cells. TV-ZnM1 enables quantitative, real-time imaging of intracellular Zn²⁺ dynamics during pyroptosis, allowing the first continuous observation of zinc redistribution throughout the entire pyroptotic process. This modular strategy offers a versatile framework for designing FRET-based sensors applicable to diverse intracellular targets, expanding the toolkit for live-cell imaging applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1405 ","pages":"Article 345470"},"PeriodicalIF":6.0,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147578129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A near-infrared ratiometric fluorescent probe for visual sensing of H2S and monitoring its fluctuation in plant roots under drought and flooding stresses","authors":"Ruxuan Mo ,&nbsp;Chao Kang ,&nbsp;Jianping Yuan ,&nbsp;Carl Redshaw ,&nbsp;Ying Huang ,&nbsp;Qilong Zhang ,&nbsp;Xiufang Yan","doi":"10.1016/j.aca.2026.345144","DOIUrl":"10.1016/j.aca.2026.345144","url":null,"abstract":"<div><h3>Background</h3><div>Hydrogen sulfide (H₂S) is a key endogenous gasotransmitter involved in plant physiological regulation and stress responses. Monitoring its dynamic changes in plants is essential for understanding related signaling mechanisms. However, due to its chemical instability and the complexity of plant tissues, developing a reliable method for accurate quantification and real-time visualization of H₂S in living plants remains challenging. Although fluorescent probes have been developed for H₂S imaging, most still face critical limitations, such as emission in the visible region being susceptible to background fluorescence interference, and insufficient quantitative reliability of single-wavelength-based signal output modes in complex samples. Therefore, developing novel probes with near-infrared emission and rationetric response characteristics is of great significance.</div></div><div><h3>Results</h3><div>We constructed a near-infrared rationetric fluorescent probe, NIR-Cou-H₂S, for H₂S detection. The probe itself emits at 716 nm, and after specific reaction with H₂S, a new emission peak appears at 552 nm, resulting in a distinct dual-emission rationetric response (716 nm/552 nm) accompanied by a visible color change. Using chemometrics-based fluorescence analysis, the probe successfully enabled direct quantitative detection of H₂S in river and lake water samples. Its near-infrared emission effectively reduced interference from plant autofluorescence, thereby achieving high-contrast dual-channel fluorescence imaging. The probe was successfully applied for high-quality in situ visualization of H₂S in living cells and tobacco seedling roots. More importantly, using NIR-Cou-H₂S, we observed and recorded in real time the dynamic upregulation trend of endogenous H₂S levels in tobacco roots under both drought and flooding stress conditions.</div></div><div><h3>Significance</h3><div>As a novel near-infrared rationetric probe, NIR-Cou-H₂S provides a powerful tool for monitoring endogenous H₂S dynamics in plants. Its characteristics significantly improve the reliability of imaging and quantification in complex plant samples, offering a key methodological approach for further elucidating the regulatory mechanisms of H₂S in plant stress resistance.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345144"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146072873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A protease-resistant and anti-fouling electrochemical biosensing interface constructed with dl-serine modified Y-shaped peptides for reliable analysis of aminopeptidase N in human serum","authors":"Yudong Wang,&nbsp;Yanxin Li,&nbsp;Yinan Zhan,&nbsp;Jie Yang,&nbsp;Yuxin Zhang,&nbsp;Lei Wang,&nbsp;Xiliang Luo","doi":"10.1016/j.aca.2026.345185","DOIUrl":"10.1016/j.aca.2026.345185","url":null,"abstract":"<div><div>Electrochemical sensing techniques are still facing the challenges of biofouling and insufficient probe stability in complex biological matrices. To address this issue, we have designed a <span>dl</span>-serine Y-shaped peptide (YPEP) integrating the antifouling properties and recognition efficiency. Application demonstration was performed with detecting aminopeptidase N (APN) in human serum. The peptide-modified sensing interface exhibits effective antifouling performance, and enhanced resistance to enzymatic degradation. When detecting APN, the developed sensor showed a sensitive response within the linear range of 1.0 ng mL⁻¹ to 10 μg mL⁻¹ and a detection limit of 0.24 ng mL⁻¹. Comparison studies were performed with the clinical human serum samples, and results obtained with the developed sensor and standard ELISA methods are in close agreement. This work provides a feasible strategy to developing robust antifouling peptide-based biosensing platforms for clinical applications in complex biological matrices.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345185"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146095760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histology-guided spatial lipidomics and proteomics of the trisynaptic circuit in the human hippocampus","authors":"Caitlin M. Tressler ,&nbsp;Lauren DeVine ,&nbsp;Rahul Bharadwaj ,&nbsp;Dalton R. Brown ,&nbsp;Daniel Weinberger ,&nbsp;Kristine Glunde ,&nbsp;Robert N. Cole","doi":"10.1016/j.aca.2026.345110","DOIUrl":"10.1016/j.aca.2026.345110","url":null,"abstract":"<div><div>The human hippocampal trisynaptic circuit activity is essential for learning and memory. This canonical circuit has spatially distinct populations of neurons, but their unique contributions to neurodevelopment, as well as to dysfunction in neurodegenerative disorders, are missed when analyzing bulk tissue homogenates. Using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) to guide laser capture microdissection (LCMD) of regions of interest for spatial multimodal analyses is a relatively new approach to study topographically distinct neuronal cell populations in heterogenous tissues. However, MALDI-MSI may not identify region-defining molecular mass-to-charge ions. Here, we apply a multimodal approach of MALDI-MSI-LCMD-lipidomic and proteomic analysis to the trisynaptic circuit. Our MALDI-MSI of the hippocampus revealed that the of mass-to-charge ions of the cornu ammonis 1 (CA1) and cornu ammonis 3 (CA3) did not segment from the surrounding tissue. Thus, we developed a novel histology-guided MALDI-MS imaging-LCMD-spatial lipidomic/proteomic pipeline with four steps which does not rely on segmentation analysis to determine and co-register regions of interest in tissue sections. Our pipeline allows MALDI imaging, LCMD, lipidomic and proteomic analysis from the same tissue section and does not require co-registration across serial sections. In addition, poly-<span>l</span>-lysine coating for improving tissue/cell adherence on indium-tin-oxide microscopy slides did not impact MALDI-MSI or spatial proteomics. We show that the human trisynaptic circuit proteomes of CA1 and CA3 pyramidal neurons are more similar to each other than those of the dentate gyrus (DG), which is consistent with previously reported transcriptomics studies. The spatial distributions of several phospholipids and proteins, however, were significantly different in cell bodies from the CA1, CA3 and DG regions, and these lipids correlated with some lipid metabolizing enzymes in those regions. As little is known about lipid metabolism in the hippocampus, our pipeline provides an initial step in studying the combined and differential spatial regulation of the lipids and proteins within the trisynaptic circuit that will provide insights into the development and disease-related molecular changes in these important hippocampal regions.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345110"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146033809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nicking-controlled biosensor: Integrating DNA Walker-RCA circuit with trimetallic nanozyme for aflatoxin B1 detection","authors":"Longsheng Jin ,&nbsp;YuJing Liu ,&nbsp;Wenqing Xia ,&nbsp;Meiling Zhou ,&nbsp;Ziying Xiao ,&nbsp;Jingyu Ge ,&nbsp;Xiao Yuan ,&nbsp;Qinqiang Zhu ,&nbsp;Meisheng Wu","doi":"10.1016/j.aca.2026.345193","DOIUrl":"10.1016/j.aca.2026.345193","url":null,"abstract":"<div><h3>Background</h3><div>Aflatoxin B₁ (AFB₁) is a highly toxic mycotoxin that poses serious threats to global food safety and public health. The ingestion of these contaminated products causes substantial risks to both food safety systems and public health. Despite the availability of various detection techniques, the requirement for highly sensitive analysis of AFB₁ contamination in agricultural products continues to drive demand for improved methodologies.</div></div><div><h3>Results</h3><div>In this work, a novel electrochemiluminescence (ECL) biosensor was developed for the sensitive detection of aflatoxin B₁ (AFB₁) by integrating a galvanic replacement–synthesized Au@Ag–Pt nanozyme with a DNA walker–controlled rolling circle amplification (RCA) circuit. The core–shell Au@Ag–Pt nanozyme exhibits superior conductivity, enhanced peroxidase activity, and electrocatalytic performance, leading to a 17.67–fold amplification of ECL. A target-responsive “signal–off” mechanism was established based on AFB₁-induced DNA structural switching and enzymatic cleavage, which effectively suppresses the RCA process and reduces the immobilization of nanozyme labels. The biosensor achieved a wide linear range from 5 × 10⁻¹¹ to 5 × 10⁻⁵ mg/mL with a detection limit of 2.72 × 10⁻¹¹ mg/mL. It shows high specificity against common interfering mycotoxins, good reproducibility, and satisfactory recoveries ranging from 82.7% to 110.5%.</div></div><div><h3>Significance</h3><div>This work not only provides a reliable platform for mycotoxin monitoring but also advances nanozyme engineering in biosensing, offering significant promise for food safety and environmental applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1392 ","pages":"Article 345193"},"PeriodicalIF":6.0,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}