Analytica Chimica Acta最新文献

{"title":"An electric field and runtime driven band model for high-speed, real-time imaging gel electrophoresis","authors":"Kan Luo ,&nbsp;Yu Chen ,&nbsp;Chaobing Liang ,&nbsp;Qirong Zhang ,&nbsp;Jing Huang ,&nbsp;Wu Wang ,&nbsp;Lina Yang","doi":"10.1016/j.aca.2025.343873","DOIUrl":"10.1016/j.aca.2025.343873","url":null,"abstract":"<div><h3>Background</h3><div>Traditional temperature-dependent models face difficulties in compact systems due to complex temperature control. This study introduces an electric field strength and runtime driven (<em>E-t</em>) band model to improve GE performance by correlating band behavior with electric field and runtime rather than temperature.</div></div><div><h3>Results</h3><div>We developed a compact <em>E-t</em> model based GE system, which equipped with inert platinum-titanium electrodes, a quartz-glass-embedded tank for passive cooling, and smartphone-based real-time fluorescence imaging. Experimental results from real-time tracking of GE bands, model fitting under different <em>E-t</em> conditions, and the separation of rice receptor protein kinase genes (CERK1 and CEBiP) confirmed that the proposed model can accurately describe electrophoretic band migration and dispersion, while maintaining good agreement with traditional temperature-based models and being little affected by temperature. Furthermore, successful nucleic acid separation was achieved within minutes under a high electric field strength in our system.</div></div><div><h3>Significance</h3><div>By minimizing the reliance on temperature control mechanisms, the <em>E-t</em> band model offers a new perspective for the design of analytical chemistry instruments, enabling electrophoresis to focus primarily on optimizing the two key parameters, <em>E</em> and <em>t</em>. In addition, our portable, real-time imaging GE system enhances separation efficiency and provides a practical, high-performance reference solution for rapid, on-site analysis applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1350 ","pages":"Article 343873"},"PeriodicalIF":5.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-time global coagulation assay via ordered porous layer interferometry using silica colloidal crystal film","authors":"Feng Wu,&nbsp;Yili Xu,&nbsp;Chenyang Ji,&nbsp;Xue Li,&nbsp;Jue Shen,&nbsp;Yejie Fei,&nbsp;Guanghan Yuan,&nbsp;Hui Qian","doi":"10.1016/j.aca.2025.343876","DOIUrl":"10.1016/j.aca.2025.343876","url":null,"abstract":"<div><h3>Background</h3><div>The coagulation process is inherently complex, and conventional diagnostic techniques are typically only capable of detecting single coagulation factors, lacking capacity to assess the overall coagulation function of the patient. Therefore, it is thus imperative to develop a novel, global coagulation assay for the diagnosis of hemorrhagic and thrombotic disorders and to evaluate the therapeutic efficacy of anticoagulant drugs. The ordered porous layer interferometry technology, developed using silica colloidal crystal (SCC) film as sensing substrate, is characterized by low cost, real-time, and convenient operation. Furthermore, entire biological events process can be detected by migration of the SCC film interference spectra.</div></div><div><h3>Results</h3><div>In this study, we developed a global coagulation assay with ordered porous layer interferometry by SCC film, a model of coagulation was created using fibrinogen and thrombin. The results demonstrated that this method could analyze the effect of different thrombin activities and different fibrinogen concentrations on the coagulation process. The performance of the coagulation assay was further validated using real blood samples and the method was found to have excellent detection capacity for different concentrations of CaCl₂, which could trigger coagulation process. Hypercoagulable and hypocoagulable samples were tested by setting different biochemical conditions, and it could distinguish blood samples from different states significantly. Clinical samples were collected and the approach was compared with standard coagulation assays with satisfactory clinical correlation.</div></div><div><h3>Significance</h3><div>This global coagulation assay is simple, rapid and real-time, and does not require various biochemical reagents. The whole process of coagulation reaction can be recorded, and multiple coagulation indicators can be obtained in one test. It is anticipated to provide a novel low-cost and powerful method for clinical coagulation testing.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1350 ","pages":"Article 343876"},"PeriodicalIF":5.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143507331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanobody based immunoassay for detection of aquatic virus: Giant salamander iridovirus","authors":"Zhihao Xu ,&nbsp;Man Liu ,&nbsp;Qiyi He ,&nbsp;Wenkai Li ,&nbsp;Junkang Pan ,&nbsp;El-Sayed A. El-Sheikh ,&nbsp;Wenzhi Liu ,&nbsp;Bruce D. Hammock ,&nbsp;Dongyang Li","doi":"10.1016/j.aca.2025.343877","DOIUrl":"10.1016/j.aca.2025.343877","url":null,"abstract":"<div><div>Aquatic viruses cause devastating diseases in aquaculture, severely limiting production and resulting in significant economic losses. The giant salamander iridovirus (GSIV), a member of the genus <em>Ranavirus</em>, is the only virus reported to infect giant salamanders, posing a severe threat to the farming industry. Currently, there are no effective strategies available for its control. In this context, a reliable diagnostic tool for the rapid detection of GSIV is crucial to mitigate its impact. This study developed a nanobody-based immunoassay for the rapid and reliable detection of GSIV. GSIV was cultured and used to immunize an alpaca, A phage library with an original diversity of 1.89 × 10⁸ PFU was established, and six nanobodies were identified after three rounds of panning. Among them, HC-2 exhibited superior performance and was used to develop a highly sensitive ELISA method employing streptavidin-PolyHRP (SA-PolyHRP) as a signal amplification strategy. The assay achieved a detection limit of 3.3 × 10⁵ PFU/mL and demonstrated high specificity without cross-reactivity. Practical application was validated in infected giant salamander samples, underscoring its diagnostic potential. This work provides a robust tool for GSIV diagnosis and showcases the potential of nanobodies in advancing aquaculture diagnostics.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1350 ","pages":"Article 343877"},"PeriodicalIF":5.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143507442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a pseudotargeted LC‒MS/MS workflow for analyzing triglycerides in biological samples","authors":"Wei-Chieh Wang ,&nbsp;Chin-Yi Wang ,&nbsp;Ta-Chen Su ,&nbsp;Po-Chih Lin ,&nbsp;Wen-Chi Chang ,&nbsp;Kuei-Pin Chung ,&nbsp;Ching-Hua Kuo","doi":"10.1016/j.aca.2025.343874","DOIUrl":"10.1016/j.aca.2025.343874","url":null,"abstract":"<div><h3>Background</h3><div>Triglycerides (TGs) play a crucial role in various physiological processes through the breakdown of their fatty acyl (FA) side chains. It has been demonstrated that not only the total levels of TGs but also the specific composition of FA side chains are vital for biological functions. However, biomedical studies that comprehensively identify FA compositions remain very limited. One of the reasons is the structural heterogeneity of TGs, with variability in their three fatty acyl chains posing significant challenges for TG analysis.</div></div><div><h3>Results</h3><div>This study proposed a pseudotargeted TG analytical workflow that generated a unique dynamic multiple reaction monitoring (dMRM) acquisition list tailored to different biological sample types.TG profiles were acquired in full scan mode using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-qToF), while LC-triple quadrupole mass spectrometry (LC-QqQ) with PIS was applied to identify fatty acyl chains. Finally, dMRM transitions were derived from confirmed ion pairs of TGs with specific FAs. Two demonstration samples, murine type 2 alveolar epithelial cell line, MLE12, with fatty acid synthase deletion, and hypertriglyceridemia plasma, were used to display the capability of the platform. While more TG species were identified in the MLE12 cell samples compared to human plasma samples (53 vs. 47), a more complex and diverse range of FA compositions in TGs was observed in human plasma compared to MLE12 cell samples (379 vs. 167).</div></div><div><h3>Significance</h3><div>Our results emphasize the need for customized MRM acquisition tailored to different biological samples, and the pseudotargeted TG analytical workflow proves effective in improving the understanding of TG regulation in biological systems. This study offers a novel and effective solution to address the complex challenges of TG analysis, enhancing accuracy, specificity, and interpretative strength.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1350 ","pages":"Article 343874"},"PeriodicalIF":5.7,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of CIC and HR GFMAS for the measurements of extractable organofluorines (EOF) in different biological tissues of pilot whales","authors":"Amnah Al Zbedy, Rudolf Aro, Abdullah Akhdhar, Viktoria Müller, Rainer Ebel, Andrew Brownlow, Gareth J. Norton, Leo W.Y. Yeung, Joerg Feldmann","doi":"10.1016/j.aca.2025.343855","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343855","url":null,"abstract":"<h3>Background</h3>In most water and biological samples, the sum of target or even non-target PFAS makes up only a small fraction of the extractable organofluorine (EOF). The methods used for EOF analysis in the methanol are combustion ion chromatography (CIC) and recently high-resolution graphite furnace molecular absorption spectrometry (HR GFMAS). Water samples show a bias towards higher concentrations measured with HR GFMAS than that of CIC. Whether the bias depends on the type of PFAS or on the sample matrix has not been known. Here we study the PFAS compound and the matrix effect using HR GFMAS and compare these with the CIC results.<h3>Results</h3>Calibration of the HR GFMAS with fluoride exhibit significant higher response than using PFOA indicating a compound specific effect. PFOA calibration gave in the average quantitative recoveries for different type of PFAS in methanol, although neutral PFAS (FTOH) show lower recoveries than PFCA using HR GFMAS. Spiked PFOA in brain and liver tissue samples resulted in quantitative recoveries (101%) using HR GFMAS. 44 liver, kidney, blubber and brain samples from stranded pilot whales were analyzed by HR GFMAS and compared to the EOF concentrations analyzed by CIC. All EOF results were above the LOD of both methods; 27 and 916 mg/kg (CIC) and between 77 and 3130 mg/kg (HR GFMAS). In general HR GFMAS gave significantly higher results for all tissues than CIC (p&gt;0.05). Correlations between the EOF of all tissues showed a tissue dependant behaviour.<h3>Significance</h3>Since PFOA recovery was in brain and liver quantitative, the results indicate that PFAS type influence the performance using HR GFMAS. Overall, we found the concentrations in the different tissues to be comparable with the two methods within one order of magnitude. This highlights the fact that more work is needed to identify the matrix effects and fluorine species dependant responses of both methods.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"66 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143507437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isomer-resolved characterization of acylcarnitines reveals alterations in type 2 diabetes","authors":"Xiangyu Gao ,&nbsp;Chunli Liu ,&nbsp;Xue Zhao","doi":"10.1016/j.aca.2025.343856","DOIUrl":"10.1016/j.aca.2025.343856","url":null,"abstract":"<div><h3>Background</h3><div>Acylcarnitines (CARs) are metabolites of fatty acids that play crucial roles in various cellular energy metabolism pathways. The structural diversity of CAR species arises from several modifications localized on the fatty acyl chain and there is currently a lack of reports characterizing these detailed structures. High-performance liquid chromatography (HPLC)-electrospray mass spectrometry (ESI-MS) is the common tool for CARs analysis.</div></div><div><h3>Results</h3><div>In this study, we improved the MS detection signals of CARs by adding NH₄HCO₃ as buffer in the mobile phase of LC system. We demonstrated that electron activated dissociation (EAD) on the ZenoTOF 7600 system is capable of localizing the hydroxyl group and methyl branching position in CARs. The benzophenone Paternò–Büchi (PB) reaction was used for derivatizing the carbon-carbon double bond (C<img>C). The capability of profiling CARs with detailed structural information was demonstrated by analyzing complex lipid extracts from mouse plasma. Our results also provided visualization of isomers composition, including branched chain isomers of CAR 4:0 and CAR 5:0 and C<img>C location isomers of unsaturated CARs. Notably, we observed significant changes in the relative compositions of branched-chain isomers of CAR 5:0 and C<img>C location isomers of several unsaturated CARs in mouse plasma samples from type 2 diabetes (T2D) compared to normal controls, suggesting their potential as diagnostic indicators for T2D.</div></div><div><h3>Significance</h3><div>In this work, we enhanced the limit of detection for acylcarnitine species by incorporating ammonium bicarbonate into the LC system. The C<img>C positions in the acyl chain of CARs were identified using Paternò–Büchi (PB) derivatization coupled with tandem mass spectrometry. Modifications such as methyl branching and hydroxyl groups along the acyl chain were localized through Electron-Activated Dissociation (EAD) on the Zeno-TOF 7600 system.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1351 ","pages":"Article 343856"},"PeriodicalIF":5.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fast screening of COVID-19 inpatient samples by integrating machine learning and label-free SERS methods","authors":"Jaya Sitjar ,&nbsp;Huey-Pin Tsai ,&nbsp;Han Lee ,&nbsp;Chun-Wei Chang ,&nbsp;Xin-Ni Wu ,&nbsp;Jiunn-Der Liao","doi":"10.1016/j.aca.2025.343872","DOIUrl":"10.1016/j.aca.2025.343872","url":null,"abstract":"<div><h3>Background</h3><div>Advances in bio-analyte detection demonstrate the need for innovation to overcome the limitations of traditional methods. Emerging viruses evolve into variants, driving the need for fast screening to minimize the time required for positive detection and establish standardized detection. In this study, a SERS-active substrate with Au NPs on a regularly arranged ZrO₂ nanoporous structure was utilized to obtain the SERS spectrum of inpatient samples from COVID-19 patients. Two analytical approaches were applied to classify clinical samples - empirical method to identify peak assignments corresponding to the target SARS-CoV-2 BA.2 variant, and machine learning (ML) method to build classifier models.</div></div><div><h3>Results</h3><div>Comparison of spectral profiles of pure BA.2 variant and inpatient samples showed significant differences in the occurrence of SERS peaks, requiring the selection of regions of interest for further analysis through the empirical method. SERS spectra are classified into CoV (+) and CoV (−) using both empirical and machine learning methods, each demonstrating a sensitivity of 85.7 % and a specificity of 60 %. The former method relies on peak assignment, which is performed manually relying on established and results in a longer turnaround time. In contrast, the latter method is based on the mathematical correlations between variables across the entire spectrum. The machine must continuously learn from larger datasets, and the response time for interpretation is short. Nonetheless, both methods demonstrated their suitability in classifying clinical samples.</div></div><div><h3>Significance</h3><div>This study demonstrated that a more comprehensive discussion can be formed with the integration of machine learning classification with biochemical profiling with the empirical analysis approach. Further improvement is expected by combining these two methods by utilizing only the regions of interest instead of the entire spectrum as input for machine learning, as a feature extraction technique.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1350 ","pages":"Article 343872"},"PeriodicalIF":5.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orthogonal DNA self-assembly technology enables rapid and accurate analysis of circulating tumor cells in breast cancer","authors":"Min Li ,&nbsp;Runchi Zhang ,&nbsp;Xiaozhi Huang ,&nbsp;Meng Jian ,&nbsp;Haipeng Qi ,&nbsp;Xiaolong Chen ,&nbsp;Jie Deng ,&nbsp;Wen Chen ,&nbsp;Chang Feng ,&nbsp;Qiongzhu Dong ,&nbsp;Bin Su","doi":"10.1016/j.aca.2025.343871","DOIUrl":"10.1016/j.aca.2025.343871","url":null,"abstract":"<div><h3>Background</h3><div>As a non-invasive liquid biopsy technology, the detection of circulating tumor cells (CTCs) overcomes the limitations of traditional tissue biopsy methods, enabling continuous sample collection and long-term dynamic monitoring. However, current CTCs analysis methods typically rely on cell size to separate and identify tumor cells, which fails to effectively distinguish tumor cells from different sources. In addition, existing methods are often constrained by limited antibody species, typically detecting only 2–3 molecular phenotypes. This narrow detection scope does not fully capture the heterogeneity of CTCs at the single-cell level, thus limiting its utility in precision diagnosis and personalized treatment. To address these challenges, it is urgent to develop CTCs detection methods that can simultaneously integrate comprehensive target and cell morphology information.</div></div><div><h3>Results</h3><div>Using breast cancer as a research model, we developed a computer-aided design-based hybridization chain reaction (CAD-HCR) by combining DNA encoding and antibody coupling technologies with orthogonal DNA self-assembly to achieve multiple detection and heterogeneity analysis of breast cancer mimic samples. This technology overcomes the limitation of antibody species in traditional CTCs detection and utilizes antibody-trigger strand coupling to convert target protein signals into DNA signals, thereby circumventing throughput limitation of existing detection methods. By utilizing the signal amplification effect of DNA self-assembly, this technology enhances sensitivity significantly, allowing for accurate single-cell level detection of CTCs.</div></div><div><h3>Significance</h3><div>This technology provides spatial positioning and cell morphological characteristics information for CTCs analysis of breast cancer, which is expected to provide a more accurate basis for diagnosis and treatment decision-making for in-depth understanding of tumor heterogeneity and clinical applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1350 ","pages":"Article 343871"},"PeriodicalIF":5.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143496147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel dual-channel fluorescent probe for the detection of peroxynitrite anions and lipid droplets in epileptic disease","authors":"Yingxin Guo,&nbsp;Huawei Huang,&nbsp;Qian Zhang,&nbsp;Hongjian Wang,&nbsp;Miaomiao Liu,&nbsp;Weiying Lin","doi":"10.1016/j.aca.2025.343863","DOIUrl":"10.1016/j.aca.2025.343863","url":null,"abstract":"<div><div>Peroxynitrite (ONOO⁻) and lipid droplets (LDs) are crucial substances essential for maintaining normal physiological functions in biological systems. They play pivotal roles as biomarkers in the initiation and progression of various diseases, such as epilepsy. Therefore, the simultaneous detection of ONOO⁻ and LDs in epilepsy disorders is of great importance. Here, we discovered that the fluorescence probe composed of trifluoromesulfonate and fluorophore can not only be used as the recognition site of ONOO⁻, but also has the property of LDs targeting. Therefore, we reasonable designed and synthesized a dual-channel fluorescent probe <strong>CBT</strong>, capable of simultaneously monitoring ONOO⁻ and LDs. <strong>CBT</strong> exhibited exceptional dual-response properties: firstly, upon specific reaction with ONOO⁻, the resulting product <strong>BHD</strong> emitted a robust red fluorescent signal in the near-infrared region (749 nm); secondly, <strong>CBT</strong> selectively targeted and labeled LDs, emitting green fluorescence at 482 nm for effective LDs tracking. The signals from these two detection channels did not overlap, which significantly enhanced the accuracy and reliability of detection. Based on these characteristics, <strong>CBT</strong> has been successfully utilized in real-time imaging of ONOO⁻ and LDs in epilepsy models of cells induced by various drugs. Notably, in a pentylenetetrazole (PTZ)-induced chronic epileptic mice model, <strong>CBT</strong> exhibited excellent efficacy in ONOO⁻ imaging, further confirming its considerable potential for practical applications. In summary, this study validated <strong>CBT</strong> as an efficient tool capable of simultaneous detection and differentiation of ONOO⁻ and LDs, presenting a novel and promising strategy for the early diagnosis and treatment of diseases such as epilepsy.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1350 ","pages":"Article 343863"},"PeriodicalIF":5.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of an electrical impedance tomography (EIT) sensor for real-time monitoring of hemolysis dynamics","authors":"Piao Peng ,&nbsp;Yue Fu ,&nbsp;Bo Che ,&nbsp;Xuan Li ,&nbsp;Lei Liu ,&nbsp;Jing Sun ,&nbsp;Teng Luo ,&nbsp;Linhong Deng","doi":"10.1016/j.aca.2025.343812","DOIUrl":"10.1016/j.aca.2025.343812","url":null,"abstract":"<div><h3>Background</h3><div>Real-time monitoring of hemolysis dynamics is essential for clinical diagnosis, ensuring transfusion safety, and supporting medical device development. Traditional methods such as spectrophotometry have limitations in real-time monitoring capabilities, often posing higher operational costs and restricted temporal resolution.</div></div><div><h3>Results</h3><div>This study presents an Electrical Impedance Tomography (EIT) sensor designed for real-time monitoring of hemolysis dynamics. The EIT sensor comprises 16 circular electrodes within a cylindrical test chamber, connected to computer-controlled hardware and software for comprehensive data acquisition and analysis. Experimental validation shows that the EIT sensor can effectively monitor and visually display the dynamic process of hemolysis, irrespective of its underlying cause. Results from EIT measurements align closely with those obtained by the conventional spectrophotometric method. Furthermore, the EIT sensor accurately detects and monitors hemolysis in real-time, even when hemolysis is induced by ultrasound, chemical reagents, or a copper-simulated blood-contacting material with a super glue surface coating, within just 20 min of contact with blood.</div></div><div><h3>Significance</h3><div>This EIT sensor represents a novel approach to hemolysis monitoring, providing valuable insights into hemolytic mechanisms, especially those related to biomaterials application. With its high temporal resolution, low cost, non-invasiveness, and portability, the EIT sensor offers a promising alternative tool for detecting and characterizing hemolysis, with potential applications in fundamental research and clinical practice, such as blood sample collection and long-term preservation.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1350 ","pages":"Article 343812"},"PeriodicalIF":5.7,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}