Analytica Chimica Acta最新文献

筛选
英文 中文
Systematic method optimisation approach for small molecule imaging on SELECT SERIES MALDI MRT - drug mapping in fingerprints, a case study
IF 6.2 2区 化学
Analytica Chimica Acta Pub Date : 2025-04-01 DOI: 10.1016/j.aca.2025.343998
Rohith Krishna, James Langridge, Emmanuelle Claude, Robert Bradshaw, Laura Cole, Simona Francese
{"title":"Systematic method optimisation approach for small molecule imaging on SELECT SERIES MALDI MRT - drug mapping in fingerprints, a case study","authors":"Rohith Krishna, James Langridge, Emmanuelle Claude, Robert Bradshaw, Laura Cole, Simona Francese","doi":"10.1016/j.aca.2025.343998","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343998","url":null,"abstract":"Molecular fingerprinting via Matrix Assisted Laser Desorption Ionisation time-of-flight Mass Spectrometry profiling (MALDI MSP) and Imaging (MALDI-MSI) has been proven to provide biometric and lifestyle information from a fingermark. As such, it can be an effective tool to assist police investigations, thus justifying continued effort to improve the quality and range of the forensic intelligence that it is possible to provide. We are currently exploring the advancements in molecular fingerprinting capabilities on the state-of-the -art SELECT SERIES MALDI-MRT mass spectrometer based on its high mass measurement accuracy (sub-ppm) and spatial resolution (15 μm). The unique nature of this instrument requires careful optimisation to maximise the system’s performance for the imaging of small molecules in fingerprints. In this study, a systematic approach to setting optimisation including optimisation of laser parameters, source voltage, quadrupole MS profile, and Transfer gas as well as RF voltage settings were determined for the analysis of latent fingerprint imaging and antipsychotics-contaminated fingerprint imaging. The developed method eventually yields significant improvement in image resolution over the default parameters.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"79 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PCR-Based SNP Genotyping: A Comprehensive Comparison of Methods for Affordable and Accurate Detection of Class IV Mutations
IF 6.2 2区 化学
Analytica Chimica Acta Pub Date : 2025-03-31 DOI: 10.1016/j.aca.2025.343994
Kajan Muneeswaran, Varuni A. de Silva, Umayal Branavan, Madhubhashinee Dayabandara, Raveen Hanwella, Naduviladath Vishvanath Chandrasekharan
{"title":"PCR-Based SNP Genotyping: A Comprehensive Comparison of Methods for Affordable and Accurate Detection of Class IV Mutations","authors":"Kajan Muneeswaran, Varuni A. de Silva, Umayal Branavan, Madhubhashinee Dayabandara, Raveen Hanwella, Naduviladath Vishvanath Chandrasekharan","doi":"10.1016/j.aca.2025.343994","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343994","url":null,"abstract":"Single nucleotide polymorphisms (SNPs) influence gene function and impact health and disease. Identifying and genotyping SNPs is crucial in various areas of research and applications. This study compared five PCR-based methods for detecting a challenging T-to-A SNP, rs9939609: ARMS-PCR, PIRA-PCR, TaqMan qPCR, CADMA with HRM, and HRM, using snapback primers. Sanger sequencing served as the gold standard.Five assays were designed and compared for genotyping rs9939609. Performance was evaluated based on affordability, ease of use, robustness, and sensitivity. Melting curve analysis was used for the CADMA and snapback primer HRM assays.All methods successfully genotyped the rs9939609 variant. ARMS-PCR was the simplest and most cost-effective method but was potentially less sensitive. PIRA-PCR offered increased sensitivity but required specific restriction enzymes, increasing cost and complexity. TaqMan qPCR was fast and sensitive but expensive due to probe requirements. The combination of CADMA with HRM balanced affordability and speed with good sensitivity and applicability to standard qPCR platforms. The snapback primer HRM offered high sensitivity but required longer assay times and careful optimization.CADMA emerged as the most balanced method, combining affordability with sensitivity and comparable to that of Sanger sequencing and TaqMan qPCR. Its effectiveness for challenging mutations and compatibility with standard qPCR platforms make it a practical choice for various laboratories. Each method offers trade-offs in cost, sensitivity, and complexity, catering to specific research and diagnostic needs. Future advancements may further refine these techniques for broader application.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"58 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Introducing an algorithm to accurately determine copolymer block-length distributions
IF 5.7 2区 化学
Analytica Chimica Acta Pub Date : 2025-03-31 DOI: 10.1016/j.aca.2025.343990
Rick S. van den Hurk , Ynze Mengerink , Ron A.H. Peters , Arian C. van Asten , Bob W.J. Pirok , Tijmen S. Bos
{"title":"Introducing an algorithm to accurately determine copolymer block-length distributions","authors":"Rick S. van den Hurk ,&nbsp;Ynze Mengerink ,&nbsp;Ron A.H. Peters ,&nbsp;Arian C. van Asten ,&nbsp;Bob W.J. Pirok ,&nbsp;Tijmen S. Bos","doi":"10.1016/j.aca.2025.343990","DOIUrl":"10.1016/j.aca.2025.343990","url":null,"abstract":"<div><h3>Background</h3><div>Copolymers are attractive for developing advanced materials with widespread applications such as medical devices, implants, or self-healing coatings for space stations and satellites. Their physical properties are tunable by controlling polymeric characteristics such as molecular weight and chemical composition. Another characteristic that has a significant influence on the material properties is the block-length distribution (BLD). Synthetic chemists can alter the BLD independently from molecular weight and chemical composition. However, analytically characterizing these BLDs, for copolymers composed out of multiple monomers, remains a huge challenge.</div></div><div><h3>Results</h3><div>In this study, an algorithm was developed that enables the accurate determination of copolymer BLDs. Copolymers were computationally simulated and fragmented by either a repeated-sampling approach or an analytical solution to obtain unbiased ground-truth data to objectively evaluate such algorithms. The performance of the novel analytical solution, coupled with an optimization algorithm, was assessed under various conditions. We have demonstrated that a trust-region-reflective algorithm yields highly accurate BLDs when fragment data up to the tetramer level is available. Although the presence of noise in the input data led to some noise in the output, it did not notably impact the overall performance of the algorithm.</div></div><div><h3>Significance</h3><div>The proposed algorithm demonstrated significant improvements over existing algorithms for the determination of copolymer BLDs. Using accurately simulated copolymer fragment data, which can be obtained through chemical reactions or physical processes, such algorithms could objectively be evaluated on their performance for the first time. These observations indicate that the proposed algorithm holds great potential for application to experimental copolymer fragment data.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 343990"},"PeriodicalIF":5.7,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of Microplastics and Nanoplastics in Environmental Water by AFM-IR
IF 6.2 2区 化学
Analytica Chimica Acta Pub Date : 2025-03-31 DOI: 10.1016/j.aca.2025.343992
Dandan Xie, Huiyao Fang, Xia Zhao, Yuan Lin, Zhaohui Su
{"title":"Identification of Microplastics and Nanoplastics in Environmental Water by AFM-IR","authors":"Dandan Xie, Huiyao Fang, Xia Zhao, Yuan Lin, Zhaohui Su","doi":"10.1016/j.aca.2025.343992","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343992","url":null,"abstract":"<h3>Background</h3>Microplastics and nanoplastics have gained worldwide attention as environmental hazards, and reliable analysis of these tiny particles is critical to accurate assessment of their impact to the environment and human health. Among the typical methods developed for analysis of microplastics, mass spectrometry-based methods are destructive and not applicable to individual nanoplastic particles, whereas vibrational spectroscopic techniques in combination with optical microscopy do not have sufficient spatial resolution needed for characterization of the much smaller nanoplastics. Therefore, a new tool is needed for the identification of nanoplastics in the environment.<h3>Results</h3>Here we report for the first time identification of individual nanoplastics in an environmental water sample directly by atomic force microscopy-infrared (AFM-IR), a spectroscopic technique with a spatial resolution of ∼100 nm. On the basis of their spectral characteristics, four different nanoplastics, including poly(3-hydroxybutyrate) (P3HB) and a bisphenol-A based epoxy, as well as microplastics of the latter and two different polyesters were identified unambiguously, and the P3HB nanoplastic particle was found to be highly crystalline. Particles of fatty acid salt, lactic acid salt and sulfate were also identified. Amide bands were observed in the spectra of some of these particles, indicative of protein contamination. In addition, a diatom and a bacterium were identified based on their IR spectra in conjunction with the morphology and elemental composition.<h3>Significance</h3>This work demonstrates that AFM-IR is a powerful tool for studying individual nanoplastic particles in environmental samples, capable of providing not only their identities but also detailed structure information.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"56 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Instant synthesis of bimetallic CuCo PBA nanozyme for efficient colorimetric immunoassay of carcinoembryonic antigen
IF 5.7 2区 化学
Analytica Chimica Acta Pub Date : 2025-03-28 DOI: 10.1016/j.aca.2025.343981
Xiaoqin Li , Huizi Lin , Xuwei Chen , Fenqiang Luo , Rong Zhang , Xiaoyan Deng , Youxiu Lin , Dianping Tang , Wenqiang Lai
{"title":"Instant synthesis of bimetallic CuCo PBA nanozyme for efficient colorimetric immunoassay of carcinoembryonic antigen","authors":"Xiaoqin Li ,&nbsp;Huizi Lin ,&nbsp;Xuwei Chen ,&nbsp;Fenqiang Luo ,&nbsp;Rong Zhang ,&nbsp;Xiaoyan Deng ,&nbsp;Youxiu Lin ,&nbsp;Dianping Tang ,&nbsp;Wenqiang Lai","doi":"10.1016/j.aca.2025.343981","DOIUrl":"10.1016/j.aca.2025.343981","url":null,"abstract":"<div><div>Colorimetric immunoassays are widely used for biomarker detection, offering advantages of simplicity, sensitivity, and cost-effectiveness. Recent advancements focus on improving the catalytic activity of nanozymes for enhancing the sensitivity and accuracy of such assays. Bimetallic CuCo Prussian blue analog (CuCo PBA) has emerged as promising candidates due to their excellent peroxidase-like activity. However, their instant synthesis and integration into immunoassays for the rapid detection of biomarkers like carcinoembryonic antigen (CEA) remain underexplored. This study presents an innovative approach using CuCo PBA nanozymes for colorimetric immunoassays with immediate generation and application. In this study, CuCo PBA nanozymes were synthesized instantly by reacting Cu<sup>2+</sup> with K<sub>3</sub>[Co(CN)<sub>6</sub>] (&lt;1 min), and their peroxidase-like activity was exploited for a colorimetric immunoassay system targeting CEA. The system demonstrated a clear blue color change upon the reaction of CuCo PBA with H<sub>2</sub>O<sub>2</sub> and 3,3′,5,5′-tetramethylbenzidine (TMB), enabling sensitive detection. The assay was optimized for various parameters, including pH, temperature, and material ratio. A linear response was obtained for CEA detection over a concentration range of 0.05–60 ng/mL with a limit of detection (LOD) of 22 pg/mL. The integration of glucose oxidase (GOx) mediated the generation of H<sub>2</sub>O<sub>2</sub>, triggering the colorimetric reaction. This instantaneous CuCo PBA-based system effectively detected CEA in human serum samples, highlighting its potential for rapid diagnostic applications. This work introduces a novel approach for rapid and sensitive colorimetric immunoassays using CuCo PBA nanozymes that are synthesized on-demand and immediately applied. The system allows for efficient CEA detection with an exceptionally low detection limit, offering great potential for clinical diagnostics. The instant generation and application of CuCo PBA nanozymes in immunoassays represent a significant advancement in point-of-care testing technologies.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 343981"},"PeriodicalIF":5.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electrochemistry sensing of ascorbic acid based on conductive metal-organic framework (Cu3(benzenehexathiol)) nanosheets modified electrode
IF 5.7 2区 化学
Analytica Chimica Acta Pub Date : 2025-03-28 DOI: 10.1016/j.aca.2025.343980
Runhao Yuan , Xiaolong Zhong , Weiming Sun , Jian Wang , Chuanhui Huang , Zhenyu Lin , Jianping Zheng
{"title":"Electrochemistry sensing of ascorbic acid based on conductive metal-organic framework (Cu3(benzenehexathiol)) nanosheets modified electrode","authors":"Runhao Yuan ,&nbsp;Xiaolong Zhong ,&nbsp;Weiming Sun ,&nbsp;Jian Wang ,&nbsp;Chuanhui Huang ,&nbsp;Zhenyu Lin ,&nbsp;Jianping Zheng","doi":"10.1016/j.aca.2025.343980","DOIUrl":"10.1016/j.aca.2025.343980","url":null,"abstract":"<div><h3>Background</h3><div>Bulk-type conductive metal-organic frameworks (c-MOFs) had been applied to modify the electrode and used in electrochemical sensing because of the high conductive properties. But which still suffer from low mass permeability, restricted active site exposure, and poor accessibility due to the coordination saturation at metal sites in the bulk-type c-MOF. Recent studies have demonstrated that transforming bulk-type MOFs into MOFs nanosheets (NSs) can maximize the exposure of active sites and mass transfer. However, c-MOF NSs have rarely been applied in electrochemical sensing.</div></div><div><h3>Results</h3><div>This study presents NSs type c-MOF Cu<sub>3</sub>(benzenehexathiol) (CuBHT), synthesized using a simple sacrificial template method. CuBHT NSs were modified onto a glassy carbon electrode (GCE) to prepare CuBHT NSs/GCE, which was then applied to sense the model target ascorbic acid (AA), the system exhibits high sensitivity of 1.521 mA mM<sup>−1</sup> cm<sup>−2</sup> and a wide linear range of 1–789 μM, low detection limit of 0.46 μM. The sensitivity is 1.90 times higher than that of bulk-type CuBHT nanoparticles (NPs) modified GCE, which can be attributed to the CuBHT NSs having more exposed Cu sites on their surfaces. CuBHT NSs/GCE was then used to monitor AA levels in human sweat during daily activities or exercise, and the results indicated high reliability compared to the vitamin C ASA kit method.</div></div><div><h3>Significance</h3><div>The design of c-MOF CuBHT NSs/GCE lead to better performance in terms of sensitivity and low detection limit in AA sensing compared to bulk-type nanoparticles. The AA sensing mechanism based on CuBHT was investigated, and the sensing system was demonstrated by detecting AA in sweat. This work advances both the fundamental understanding and practical applications of c-MOF NSs in AA sensing.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1353 ","pages":"Article 343980"},"PeriodicalIF":5.7,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143723441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electrochemical detection of creatinine at picomolar scale with an extended linear dynamic range in human body fluids for diagnosis of kidney dysfunction
IF 5.7 2区 化学
Analytica Chimica Acta Pub Date : 2025-03-27 DOI: 10.1016/j.aca.2025.343978
Sanya Khursheed , Sumera Sarwar , Dilshad Hussain , Muhammad Raza Shah , Jiri Barek , Muhammad Imran Malik
{"title":"Electrochemical detection of creatinine at picomolar scale with an extended linear dynamic range in human body fluids for diagnosis of kidney dysfunction","authors":"Sanya Khursheed ,&nbsp;Sumera Sarwar ,&nbsp;Dilshad Hussain ,&nbsp;Muhammad Raza Shah ,&nbsp;Jiri Barek ,&nbsp;Muhammad Imran Malik","doi":"10.1016/j.aca.2025.343978","DOIUrl":"10.1016/j.aca.2025.343978","url":null,"abstract":"<div><h3>Background</h3><div>Creatinine levels in different body fluids can serve as an important biomarker for kidney functioning relevant to prostate cancer and chronic kidney disease (CKD). Creatinine levels vary in concentration in different body fluids, such as blood, urine, and saliva. Unlike previously reported sensors, the developed creatinine sensor can be employed for all levels of creatinine in samples of real patients.</div></div><div><h3>Results</h3><div>In this study, an efficient voltammetric sensor for creatinine is developed by modifying a glassy carbon electrode (GCE) with poly (ethyleneimine) (PEI) capped silver nanoparticles at titanium dioxide (PEI-AgNPs)/TiO<sub>2</sub>, i.e., titanium dioxide (TiO<sub>2</sub>)/graphene oxide (GO) nanocomposites (Ag@GO/TiO<sub>2</sub>-GCE). The Ag@GO/TiO<sub>2</sub> nanocomposite was characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), thermal gravimetric analysis (TGA), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta potential, Fourier transform infrared (FT-IR) spectroscopy, and UV–Vis spectrophotometry. Various voltammetric techniques namely cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), chronoamperometry (CA), and differential pulse voltammetry (DPV) were employed. The Ag@GO/TiO<sub>2</sub>-GCE demonstrated good selectivity, stability, and a quick response time of 1.0 s for creatinine. An extended linear dynamic range (LDR) of creatinine from 0.01 pM (DPV) to 1.0 M (CV) based on different voltammetric techniques is imperative for detecting diverse creatinine levels in various body fluids. The LOD and LOQ of the developed creatinine detection method were found to be 1.15 pM and 3.5 pM, respectively. The electrochemical sensor exhibited an exceptionally high sensitivity of 15.74 μApM<sup>−1</sup>cm<sup>−2</sup>.The body fluids from healthy volunteers were spiked with a known amount of creatinine to evaluate sensor efficiency in the context of recovery. Finally, blood serum, saliva, and urine samples of kidney patients were analyzed for creatinine levels.</div></div><div><h3>Significance</h3><div>An important merit of the developed creatinine sensor is its ability for non-invasive point-of-care diagnosis in saliva with more than 90 % recovery. The comparison of the developed method with the standard Jaffes' colorimetric method endorsed its reliability and extended ability for the samples where Jaffes’ method fails. The low LOD, high sensitivity, extended LDR, and low-cost render the possibility of adopting this method for point-of-care diagnosis.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1353 ","pages":"Article 343978"},"PeriodicalIF":5.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143713331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rational design and pairing of single-domain antibodies for developing rapid detection methods for botulinum neurotoxins A and B
IF 5.7 2区 化学
Analytica Chimica Acta Pub Date : 2025-03-27 DOI: 10.1016/j.aca.2025.343977
Ruomei Lv , Jiaxin Li , Xinyu Fang , Li Tang , Rui Shi , Jin Wang , Wenjing Zhang , Jinglin Wang , Guangyu Zhao , Jing Wang , Shan Gao , Xuefang Xu , Lin Kang , Wenwen Xin
{"title":"Rational design and pairing of single-domain antibodies for developing rapid detection methods for botulinum neurotoxins A and B","authors":"Ruomei Lv ,&nbsp;Jiaxin Li ,&nbsp;Xinyu Fang ,&nbsp;Li Tang ,&nbsp;Rui Shi ,&nbsp;Jin Wang ,&nbsp;Wenjing Zhang ,&nbsp;Jinglin Wang ,&nbsp;Guangyu Zhao ,&nbsp;Jing Wang ,&nbsp;Shan Gao ,&nbsp;Xuefang Xu ,&nbsp;Lin Kang ,&nbsp;Wenwen Xin","doi":"10.1016/j.aca.2025.343977","DOIUrl":"10.1016/j.aca.2025.343977","url":null,"abstract":"<div><h3>Background</h3><div>Botulinum neurotoxin (BoNT) is a toxin produced by <em>Clostridium botulinum</em> that can cause neurological dysfunction in humans and animals, with BoNT/A and BoNT/B being responsible for the majority of poisoning cases. Due to its extremely high toxicity, BoNT is considered a potential biological warfare and bioterrorism agent. Currently, there is a lack of rapid and highly sensitive detection methods for botulinum toxin, making the development of fast and effective detection methods for BoNT essential. Here, we developed rapid detection methods for BoNT/A and BoNT/B based on nanobodies, including two ELISA methods and Time-resolved fluorescence immunochromatography assay (TRFICA).</div></div><div><h3>Results</h3><div>By analyzing the binding epitopes of BoNT/A, BoNT/B, and nanobodies, we rationally designed and prepared six nanobodies, adding a Trx tag at the N-terminus, a 6 × His tag at the C-terminus, and fusing them with HRP, for use in developing two ELISA methods for BoNT/A and BoNT/B detection. Additionally, TRFICA was developed in combination with lanthanide microspheres. These three methods were validated using simulated samples containing BoNT in milk, sand, and human serum. The limits of detection for ELISA 1 and 2 were 0.17 and 4.5 ng/mL, respectively, while the TRFICA had a detection limit of 0.05 ng/mL and provided results in approximately 15 min. TRFICA also successfully detected clinical samples, with a sensitivity of 98 % and a specificity of 96.7 %.</div></div><div><h3>Significance</h3><div>Overall, the TRFICA method we established has a short detection time, is easy to operate, and effectively addresses the challenge of rapid BoNT detection in clinical diagnostics, with significant clinical application value. Furthermore, based on the sequences and binding epitopes of nanobodies, we rationally designed six nanobodies and successfully applied them in the development of various BoNT detection methods. This approach and pipeline provide valuable guidance for the future development of rapid, low-cost detection methods.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 343977"},"PeriodicalIF":5.7,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143713330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineering Bio-Inspired Nanochannels with pH-Responsive Gates for Direct Detection of Glucose in Human Blood Serum
IF 6.2 2区 化学
Analytica Chimica Acta Pub Date : 2025-03-26 DOI: 10.1016/j.aca.2025.343973
Yinde Wu, Jialun Li, Yunzhou Chen, Xuancheng Liu, Yueyang Zhang, Chongxuan Xiao, Zihao Li, Yan-Yan Song, Tian Shen, Xiaona Li
{"title":"Engineering Bio-Inspired Nanochannels with pH-Responsive Gates for Direct Detection of Glucose in Human Blood Serum","authors":"Yinde Wu, Jialun Li, Yunzhou Chen, Xuancheng Liu, Yueyang Zhang, Chongxuan Xiao, Zihao Li, Yan-Yan Song, Tian Shen, Xiaona Li","doi":"10.1016/j.aca.2025.343973","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343973","url":null,"abstract":"<h3>Background</h3>An abnormal blood glucose (Glu) level is a key signal of diabetes. The gluconic acid produced by Glu catalytic oxidation can cause changes in pH value.<h3>Results</h3>Inspired by nature, in which organisms use pH as a chemical gate to regulate ion transport through cell membranes, we report a pH-gated electrochemical luminescence (ECL) sensing system for Glu detection based on the relationship between Glu metabolism and pH. The pH gate was designed on a TiO<sub>2</sub> nanochannel membrane (NM) by modifying the channel entrance with polystyrene-b-poly(4-vinylpyridine) (P4VP) chains that convert from a hydrophobic into a hydrophilic state via a pH-responsive conformational switching at pKa 5.2. Due to the nanoconfinement effect of zeolite imidazolate (ZIF-8) frameworks and TiO<sub>2</sub> nanochannels, the glucose oxidase (GOD) embedded in ZIF-8 exhibits enhanced catalytic efficiency for Glu oxidation, enabling the acidic product to regulate the hydrophilicity of the P4VP-based pH-responsive gate. The ECL luminophore Ru(dcbpy)<sub>3</sub><sup>2+</sup> subsequently passes through the hydrophilic gate to reach the detection cell. This pH-gated Glu-responsive NM can effectively separate biological matrices from the detection cell, allowing direct sensing of Glu in complex biomatrices.<h3>Significance</h3>The ECL technology, combined with the pH-triggered gate design enables straightforward Glu determination in undiluted serum, demonstrating an alternative ECL device for pretreatment-free clinical analysis.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"11 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143713335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Raman spectroscopic detection of synthetic cannabinoid-related 1H-indazole-3-carboxamide on seized paper stamps: A forensic challenge in providing nondestructive analyses and feasible counterevidence
IF 5.7 2区 化学
Analytica Chimica Acta Pub Date : 2025-03-26 DOI: 10.1016/j.aca.2025.343975
Luciano S.F. Silva , Lucas S. Abreu , Eliani Spinelli , Glaucio B. Ferreira , Lucas M. Duarte
{"title":"Raman spectroscopic detection of synthetic cannabinoid-related 1H-indazole-3-carboxamide on seized paper stamps: A forensic challenge in providing nondestructive analyses and feasible counterevidence","authors":"Luciano S.F. Silva ,&nbsp;Lucas S. Abreu ,&nbsp;Eliani Spinelli ,&nbsp;Glaucio B. Ferreira ,&nbsp;Lucas M. Duarte","doi":"10.1016/j.aca.2025.343975","DOIUrl":"10.1016/j.aca.2025.343975","url":null,"abstract":"<div><h3>Background</h3><div>Synthetic cannabinoids challenge forensic laboratories, which typically rely on sample-consuming chromatography-mass spectrometry. Criminalistic regulations mandate preserving seized material for potential judicial counterevidence requests. Raman spectroscopy emerges as a promising detection method, maintaining the chemical integrity of evidence for future examinations.</div></div><div><h3>Results</h3><div>This study utilized two distinct Raman spectroscopy methodologies to detect 1H-indazole-3-carboxamide (core-linker) in synthetic cannabinoids found on seized paper stamps: 785 nm dispersive Raman microscope (direct analysis) and 1064 nm FT-Raman (with difference spectroscopy). Both methods confirmed seven core-linker bands, corroborated by Density Functional Theory calculations. Gas Chromatography-Mass Spectrometry, Electrospray Ionization Mass Spectrometry, and Proton Nuclear Magnetic Resonance analyses confirmed the presence of ADB-5′Br-butinaca, representing its first documented identification in Rio de Janeiro, Brazil.</div></div><div><h3>Significance</h3><div>The present investigation demonstrates Raman spectroscopy's efficacy for non-destructive detection of synthetic cannabinoids in seized materials. By identifying a core-linker and confirming ADB-5′Br-butinaca, it addresses the forensic challenge of preserving evidence while enabling rapid analysis. Both vibrational spectroscopy approaches offer highly effective tools for selective structural elucidation of this globally expanding class of psychoactive substances.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1353 ","pages":"Article 343975"},"PeriodicalIF":5.7,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143713332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信