Analytica Chimica Acta最新文献

{"title":"Chlorine-functionalized black phosphorus quantum dots induced superoxide anion generation and depletion for efficient chemiluminescence detection","authors":"Hui Gong, Dayang Zhao, Houjing Liu","doi":"10.1016/j.aca.2025.343991","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343991","url":null,"abstract":"<h3>Background</h3>Due to their unique optoelectronic properties, environmental friendliness, and excellent biocompatibility, metal-free quantum dots have been a new star in exploring novel chemiluminescence (CL) systems for analytical applications in recent years. However, unknown CL property, relatively weak emission and instability of some of them in water (eg. black phosphorus) often seriously hindered their further applications. Hence, developing a novel QDs-assist CL signal amplification to achieve efficient analyst detection is significant and currently hot topic for researchers.<h3>Results</h3>In this work, we purposely synthesized chlorine-functionalized black phosphorus quantum dots (Cl-BPQDs) with improved stability and rich-hole property, which were demonstrated to exhibit the excellent capability for the activation of ferrate (VI) with large reactive oxygen species generation and leading to enhanced CL signal. The detail mechanism was demonstrated, the unique CL response to the presence of active sites (P-Cl) in Cl-BPQDs, which accelerated ferrate (VI) decomposition and produced a large amount of superoxide anion (^•O₂^-). And then, the radiative recombination of the exogenous electron-donated and existing holes Cl-BPQDs accounting for the strong CL emission. Furthermore, based on the consumption capacity of ascorbic acid (AA) and glutathione (GSH) for ^•O₂^-, a direct CL sensing platform of Cl-BPQDs/ferrate (VI) quenching was fabricated to AA and GSH detection. This fabricated assay has broad detection linear ranges (2-200 μM) and low detection limit (GSH: 1.3 μM; AA: 1.7 μM). Compared with the reported CL technique, this new method displayed superior sensitivity and anti-interference capabilities toward transition-metal ions and inorganic anions. The potential analytical application of the new CL system was further demonstrated by the evaluation of total antioxidant capacity (TAC) in diabetic patients.<h3>Significance</h3>This study proposes a new strategy for enhancing CL signal via Cl-BPQDs triggering ^•O₂^- generation and depletion, which provides an innovative tool for ascorbic acid and glutathione detection. This method not only enriches our understanding of the optical characteristics of BP, but also provides a new charge transfer-based path for CL amplification.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"73 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AFM-optimized Single-Cell Level LA-ICP-MS Imaging for Quantitative Mapping of Intracellular Zinc Concentration in Immobilized Human Parietal Cells using Gelatin Droplet-Based Calibration","authors":"Valerie Boger, Philip Pirkwieser, Noreen Orth, Melanie Koehler, Veronika Somoza","doi":"10.1016/j.aca.2025.343999","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343999","url":null,"abstract":"<h3>Background</h3>Quantitative bioimaging of trace elements at the single-cell level is crucial for understanding cellular processes, including metal uptake and distribution. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has emerged as a gold standard for elemental bioimaging due to its high sensitivity and spatial resolution. However, calibration remains challenging due to the lack of homogeneous biological standards. This study addresses these challenges by introducing a gelatin-based calibration strategy optimized for Zn mapping in human parietal cells. By minimizing heterogeneity in gelatin standards and optimizing laser ablation conditions, the approach ensures accurate and reproducible results for cellular bioimaging.<h3>Results</h3>A gelatin-based calibration strategy for LA-ICP-MS was developed to quantify intracellular Zn at a single-cell level in human parietal cells. Preparation conditions for gelatin standards were optimized to minimize heterogeneity, eliminating the need for entire droplet ablation and significantly reducing analysis time. Atomic force microscopy (AFM) was employed to optimize laser ablation conditions and determine ablated volumes, ensuring quantitative Zn detection. The method demonstrated high linearity (R² &gt; 0.99) and reproducibility. Application of the calibration strategy to ZnCl₂-treated parietal cells revealed Zn distribution at a cellular level, visualized using a 5 μm laser beam. Integration with bright field imaging enabled the exclusion of apoptotic cells and debris, ensuring robust analysis. Validation with bulk ICP-MS showed excellent agreement, confirming the method’s reliability and potential for high-resolution bioimaging.<h3>Significance</h3>This work introduces a robust and reproducible calibration strategy for quantitative elemental bioimaging using LA-ICP-MS. It details the preparation of a gelatin matrix with a homogeneous element distribution, serving as an alternative to using biological material and significantly reducing analysis time. Laser ablation parameters were optimized using AFM to ensure quantitative ablation, which is necessary for calibration through LA-ICP-MS imaging. This approach provides a powerful tool for studying trace element dynamics in single cells and holds potential for diverse biological and biomedical applications.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"18 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of medium- and long-chain perfluorocarboxylic acids in water by catalytic methylation with trimethylsilyl diazomethane and gas chromatography - mass spectrometry analysis","authors":"Guoliang Huang, Baoshan Zang, Yunna Li, Guohua Zhu, Kezhi Jiang","doi":"10.1016/j.aca.2025.344001","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344001","url":null,"abstract":"Perfluorocarboxylic acids (PFCAs) have received wide public concern, due to their biotoxicity and universality. However, the current LC-MS method suffers from the background interference originating from the instrumental system and the time-consuming procedure in analyzing long-chain PFCAs. In this work, we established a rapid method for the determination of medium- and long-chain PFCAs based on derivatization with trimethylsilyl diazomethane (TMSD) and GC-MS analysis. Under the catalysis of trimethylchlorosilane, methylation of trace PFCAs with TMSD was fully achieved within only 2 min, which represents inaugural efficient methylation of carboxylic acid. The methylated products of 9 PFCAs (C7-C18 except C15 and C17) were determined by GC-MS/MS within a GC run of 13 min. Moreover, this method successfully avoided the background interference in the HPLC-MS analysis, which made analytical results more reliable. The quantitative linear range was 0.1 - 100 ng/mL for medium PFCAs (C8 to C13), 1 - 1000 ng/mL for C7 and long-chain PFCAs (C14 to C18), respectively. Good reproducibility was obtained for all PFCAs, with an intra-day RSD of 0.90 - 4.35% and an inter-day RSD of 2.62 - 5.64% at 5 ng/mL. The recovery rate was 83.2-111.3% at 10 ng/mL, indicating the accuracy and reliability of these results. These results indicated that this method provided a practical and reliable method for the detection of medium- and long-chain perfluorocarboxylic acids in water samples by GC-MS analysis.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"34 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assembling metal nanoclusters with high luminescence performance and anti-interference ability for sensing p-nitrophenol","authors":"Jiaxi Wang, Jiaqing Li, Yuexiang Lu, Xiwen Zhang, Huashuo Dou, Yueying Liu","doi":"10.1016/j.aca.2025.344009","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344009","url":null,"abstract":"<h3>Background</h3>The fluorescence quantum yield (QY) of water-soluble gold nanoclusters (AuNCs) can be prominently improved by adding L-arginine (Arg) owing to rigidifying ligands. However, these fluorescence systems exhibit poor anti-interference ability in sensing application due to exposure guanidine, amino, and carboxyl groups of Arg.<h3>Results</h3>We have developed the encapsulation of AuNCs into zeolitic imidazolate framework-8 (ZIF-8) to improve the anti-interference property due to the protection of Arg functional groups. The ligand of 6-aza-2-thiothymine (ATT)-decorated AuNCs (ATT-AuNCs) is rigidified after introducing Arg (referred as Arg/ATT-AuNCs), yielding higher QY of 59.31% compared to ATT-AuNCs with QY of 1.18%. Subsequently, the shell of Arg/ATT-AuNCs encapsulated into ZIF-8 (Arg/ATT-AuNCs@ZIF-8) does not only tremendously increase green fluorescence emission, but also enhance the anti-interference capability (high salt concentration, pH change, metal ions coordination, and solution dilution) due to the structural confinement effect and protection of ligand functional groups. Interestingly, Arg/ATT-AuNCs@ZIF-8 fluorescence can be quenched by p-nitrophenol (PNP) through an internal filtration effect (IFE). Thus, a fluorescence method is established for PNP analysis. The linear detection range for PNP is 0.1 to 80 μM with limit of detection (LOD) at 0.033 μM, which is much lower than the maximum allowable value (0.43 μM) in drinking water by EPA. This approach has been successfully applied to the detection of PNP spiked into the real samples with excellent recovery rates. This platform opens a broad avenue for metal nanocluster-based materials in the sensing application.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"31 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization, validation, and implementation of a new method for detecting liquid crystal monomers in dust using GC−MS/MS with atmospheric pressure chemical ionization","authors":"Jing Han ,&nbsp;Jun Liu ,&nbsp;Huiyuan Lu ,&nbsp;Kehan Guo ,&nbsp;Fei Zhang ,&nbsp;Shujie Guo ,&nbsp;Xianfa Su ,&nbsp;Shuying Dong ,&nbsp;Jianhui Sun ,&nbsp;Jinglan Feng ,&nbsp;Taicheng An","doi":"10.1016/j.aca.2025.344002","DOIUrl":"10.1016/j.aca.2025.344002","url":null,"abstract":"<div><h3>Background</h3><div>Liquid crystal monomers (LCMs) are a new class of emerging pollutants. To assess their occurrence, behaviour, and potential risks, a sensitive and selective analytical method is required for the determination of LCMs at trace levels in multiple environmental media. Toward this end, an improved GC−MS/MS method was developed and validated for the quantification of LCMs.</div></div><div><h3>Results</h3><div>The method integrates atmospheric pressure chemical ionization (APCI) with GC−MS/MS. Under optimal instrumental conditions, the instrument quantification limits of LCMs reached as low as 0.02 pg·injection⁻¹, which can be attributed to the generation of high-abundance molecular ions/quasi-molecular ions under APCI. Compared to previously published methods, the developed method in this study reduces the method detection limits of LCMs by approximately 1–38.7 times, enabling the analysis of LCMs at concentrations as low as 0.02 ng·g⁻¹ in dust samples. This improved approach was applied to both indoor and outdoor dust samples. The concentrations of LCMs obtained in this study are consistent with those reported in previous research, demonstrating high detection frequencies of fluorinated LCMs and their predominance in dust.</div></div><div><h3>Significance</h3><div>The developed method in this study is not only applicable to dust samples but also readily extends to other environmental matrices, thereby facilitating the investigation of the occurrence, origin, and migration of LCMs in various environments.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 344002"},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A parallel reaction monitoring–mass spectrometric method for studying lipid biosynthesis in vitro using 13C16-palmitate as an isotope tracer","authors":"Kyeong-Seog Kim ,&nbsp;Young Gyun Ko ,&nbsp;Woo Seok Yang ,&nbsp;Hye Young Kim ,&nbsp;Joo-Youn Cho","doi":"10.1016/j.aca.2025.344003","DOIUrl":"10.1016/j.aca.2025.344003","url":null,"abstract":"<div><h3>Background</h3><div>Palmitate, which is the end product of fatty acid synthase, is the key fatty acid for understanding of lipid biosynthetic process in mammalian cells. Mass spectrometry (MS) methodology using ¹³C-palmitate can trace the lipid biosynthesis such as glycerolipids, glycerophospholipids, and sphingolipids. However, due to the interferences of natural heavy isotopes, accurate measurement of ¹³C-labeled lipid species has been limited. Here we describe a high-throughput isotope tracing experiment to assess lipid biosynthesis using parallel reaction monitoring–MS (PRM–MS) with ¹³C₁₆-palmitate as an isotope tracer.</div></div><div><h3>Results</h3><div>The developed method can trace 14 ¹³C₁₆-labeled lipid classes without disturbance from the heavy isotope patterns of natural lipids. Lipid class-based separation was achieved through hydrophilic interaction liquid chromatography (HILIC) which allows facile identification of lipid, and PRM–MS was performed for accurate detection of the ¹³C₁₆-labeled lipids. A fibroblast (NIH/3T3) cell line was used as an <em>in vitro</em> model, and the NIH/3T3 cells were treated with bovine serum albumin (BSA)-bound ¹³C₁₆-palmitate. The isotopic disturbance from natural lipid was eliminated using ¹³C₁₆-palmitate, rather than ¹³C₁-palmitate, as an isotope tracer. After 24 h of incubation with 0.1 mmol/L of BSA-bound ¹³C₁₆-palmitate in the fibroblasts, NIH/3T3 cells synthesized the 127 ¹³C₁₆-labeled lipid species of glycerolipids, glycerophospholipids, and sphingolipids. Finally, in the NIH/3T3 cells incubated for 1, 6, and 24 h after the treatment of the isotope tracer exhibited an increased profile of ¹³C₁₆-labeled lipidome, depending on duration of incubation.</div></div><div><h3>Significance</h3><div>The HILIC/PRM–MS method using ¹³C₁₆-palmitate as an isotope tracer enables identification of ¹³C₁₆-labeled lipid species by annotating ¹³C₁₆-labeled position, including the ¹³C₁₆-fatty acyl chain and ¹³C₁₆-sphingolipid headgroup, without interference of natural heavy isotope patterns. This lipidomic flux analysis using PRM approach is expected to provide insights into assessment of isotope-labeled lipids.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 344003"},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure Elucidation and Discrimination of Peptides Epimers Induced by Chiral Residue by Ion Mobility Mass Spectrometry","authors":"Jianglong Du, Shutong Yang, Yanqiu Chu, Yinghua Yan, Zhenhua Li, Chuan-Fan Ding","doi":"10.1016/j.aca.2025.344000","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344000","url":null,"abstract":"<h3>Background</h3>The chirality of amino acid residues in peptide or protein affects the biological activity and function. Improving the elucidation and discrimination of peptide epimers induced by chiral residues in different positions is of great significance in the physiological and pathological study of the dominant L-chiral protein biosphere. The characterization of peptide epimers have been and remain a challenging task because chiral residues do not cause any change in the physical and chemical properties, such as sequence, isoelectric point, or mass-charge ratio.<h3>Result</h3>In this work, we provide ion mobility mass spectrometry (IM-MS) analysis of peptide epimers induced by chiral residue and further develop a general and efficient strategy for constructing non-covalent complexes with cucurbituril (CB) to achieve epimers discrimination, especially with limited resolving power of most current instruments. Specifically, IM-MS analysis probes the conformational landscape profile from the model methionine enkephalin (ME) epimers, oligomers, metal adjuncts to multi-nary complexes, improving the identification by amplify structural differences and enrich conformational features, in which [ME+CB[8]+Li+H]2+ enables simultaneous acquisition of the unique conformational feature of all ME epimers. The structural stability changes from epimers to complexes were characterized by energy resolved MS2, and further DFT calculations demonstrated the effect of non-covalent interaction on conformational differences, providing insights for molecular recognition. Significance: The method has been successfully applied to the identification of peptide epimers in neuropeptides with single chiral residue and amyloid truncated peptides with multiple chiral residues. This work provides possibility for rapid discovery and efficient identification of peptide isomers and method support for the establishment of comprehensive biomolecular collision cross section (CCS) database.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"22 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separation of Virus Like Particles and Nano-Emulsions for Vaccine Development by Capillary Zone Electrophoresis","authors":"Yousef S. Elshamy, Caleb Kinsey, Richard R. Rustandi, Adam T. Sutton","doi":"10.1016/j.aca.2025.344011","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344011","url":null,"abstract":"<h3>Background</h3>Nano-emulsions with immunogenic properties can be incorporated into vaccines to act as an adjuvant where they can enhance the immune response of a given vaccine. Analytically, studying vaccine antigens, such as Virus-Like Particles (VLPs), in the presence of adjuvants, like nano-emulsions, is very challenging as they are both heterogenous nano species of similar sizes but very different physiochemical properties. Therefore, typical analysis of nanoparticles using separation approaches such as Size Exclusion Chromatography (SEC) and Field-Flow Fractionation (FFF) is difficult due to the size similarities among these nano-species which complicates their separation.<h3>Results</h3>In this study, a Capillary Zone Electrophoresis (CZE) method was developed, which utilizes a separation mechanism based on the charge-to-size ratio of the analytes. The method was used to quantify VLPs of the Human Papilloma Virus (HPV) and Squalene Nano-Emulsion (SNE) adjuvant mixtures while also measuring buffer excipients, chloride and histidine. The method was assessed according to International Conference on Harmonization (ICH Q2) guidelines with respect to linearity, ranges, accuracy (87-109 %), precision (≤ 20%), quantitation and detection limits.<h3>Significance</h3>This study was conducted to prove the feasibility of utilizing CZE to characterize VLPs and SNE mixtures with dilution as the only sample preparation. The CZE conditions are simpler than other CZE conditions suggested for VLPs and easily transferred between users. Similar CZE methods could also be developed for other vaccine and adjuvant mixtures as well as other emulsion and nanoparticle-based systems.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"67 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic method optimisation approach for small molecule imaging on SELECT SERIES MALDI MRT - drug mapping in fingerprints, a case study","authors":"Rohith Krishna, James Langridge, Emmanuelle Claude, Robert Bradshaw, Laura Cole, Simona Francese","doi":"10.1016/j.aca.2025.343998","DOIUrl":"https://doi.org/10.1016/j.aca.2025.343998","url":null,"abstract":"Molecular fingerprinting via Matrix Assisted Laser Desorption Ionisation time-of-flight Mass Spectrometry profiling (MALDI MSP) and Imaging (MALDI-MSI) has been proven to provide biometric and lifestyle information from a fingermark. As such, it can be an effective tool to assist police investigations, thus justifying continued effort to improve the quality and range of the forensic intelligence that it is possible to provide. We are currently exploring the advancements in molecular fingerprinting capabilities on the state-of-the -art SELECT SERIES MALDI-MRT mass spectrometer based on its high mass measurement accuracy (sub-ppm) and spatial resolution (15 μm). The unique nature of this instrument requires careful optimisation to maximise the system’s performance for the imaging of small molecules in fingerprints. In this study, a systematic approach to setting optimisation including optimisation of laser parameters, source voltage, quadrupole MS profile, and Transfer gas as well as RF voltage settings were determined for the analysis of latent fingerprint imaging and antipsychotics-contaminated fingerprint imaging. The developed method eventually yields significant improvement in image resolution over the default parameters.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"79 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A prototype low-pressure assisted microwave plasma ionization mass spectrometry for on-line monitoring of organic and inorganic hazardous compounds simultaneously: Design and feasibility validation","authors":"Gaosheng Zhao,&nbsp;Yuliang Huang,&nbsp;Bin Jia,&nbsp;Jiafan Ji,&nbsp;Ping Cheng","doi":"10.1016/j.aca.2025.344004","DOIUrl":"10.1016/j.aca.2025.344004","url":null,"abstract":"<div><h3>Background</h3><div>The detection of harmful atmospheric gases, including inorganic small molecules (e.g., hydrogen sulfide, H₂S) and volatile organic compounds (VOCs) such as aromatic hydrocarbons, is crucial due to their involvement in photochemical reactions that generate secondary pollutants like ozone and secondary organic aerosols. Current mass spectrometry-based techniques, including PTR-MS, SPI-MS, and SIFT-MS, are limited in their ability to simultaneously and sensitively detect a broad spectrum of these compounds due to significant variations in their physicochemical properties.</div></div><div><h3>Results</h3><div>This study developed a low-pressure assisted microwave plasma ionization time-of-flight mass spectrometer (LAMP-TOFMS), which features a novel coaxial three-metal-tube design adapted from atmospheric-pressure microwave plasma torch technology. Operable under low-pressure conditions (10–1000 Pa), the LAMP ion source employs multiple ionization mechanisms, including Penning ionization and charge transfer. The system was optimized for key parameters such as focusing electrode voltage, microwave power, and gas flow. LAMP-TOFMS achieved sub-ppbv detection limits, a broad linear dynamic range, and high stability, enabling the detection of challenging compounds, including H₂S, cyclohexane, and <em>n</em>-heptane. It was successfully applied for the quantification of odorous compounds around a pig farm.</div></div><div><h3>Significance and novelty</h3><div>To our knowledge, this is the first study to develop a low-pressure microwave plasma ionization source capable of simultaneously detecting organic and inorganic hazardous gases in real-time. Compared to conventional methods, LAMP-TOFMS demonstrates superior sensitivity, rapid response, and broader compound coverage. This innovative approach offers a transformative solution for comprehensive atmospheric monitoring, with significant implications for air quality and environmental health research.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1354 ","pages":"Article 344004"},"PeriodicalIF":5.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}