Analytica Chimica Acta最新文献

{"title":"Turning Waste into Wealth: Enzyme-Activated DNA Sensor Based on Reactant Recycle for Spatially Selective Imaging MicroRNA toward Target Cells","authors":"Yaokun Xia, Guomin Ju, Yongting Zhou, Xiao Li, Yufei Luo, Chuanhui Peng, Xueling Liu","doi":"10.1016/j.aca.2024.343557","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343557","url":null,"abstract":"<h3>Background</h3>Amplified imaging of microRNA (miRNA) in cancer cells is essential for understanding of the underlying pathological process. Synthetic catalytic DNA circuits represent pivotal tools for miRNA imaging. However, most existing catalytic DNA circuits can not achieve the reactant recycling operation in cells and in vivo. Additionally, specificity miRNA imaging in tumor site also is a challenge. Herein, inspired by “turning waste into wealth”, we report a DNA sensor for imaging of miRNA in target cells based on apurinic/apyrimidinic endonuclease 1 (APE1)-activated reactant recycling catalytic DNA circuit.<h3>Results</h3>The sensing function of the DNA sensor is suppressed firstly through engineering an apurinic/apyrimidinic (AP) site. In the presence of APE1, the AP site undergoes hydrolysis, thereby activating sensing activity and triggering the strand displacement reaction (SDR) under miRNA assistance. In this catalytic DNA circuit, the next reaction cycle can be initiated when the duplex strand waste products are reverted into active components, which allows it to be performed continuously just consuming fuel DNA without depleting the reactant. Noteworthy, the liposome plays important role in overcome biological barriers for nucleic acid delivery. The amplified miRNA imaging strategy is carried out in vivo by this DNA sensor with reducing off-tumor signal under assistance with APE1, and enhances tumor-to-normal tissue contrast compared with traditional catalytic DNA circuit.<h3>Significance and novelty</h3>Firstly, APE1-activated reactant recycling catalytic DNA circuit is developed. Secondly, miRNA image in cell and in animal is achieved with high spatial selectivity. Thirdly, the signal-to-background ratio for imaging is improved in vitro and in vivo. Lastly, our strategy provides an automatic yet versatile approach for the development of more efficient and selective DNA circuits capable of differentiating miRNA in cancer cells from those in normal cells, promising to be valuable in cancer diagnosis.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"30 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A label-free electrochemical biosensor based on graphene quantum dots-nanoporous gold nanocomposite for highly sensitive detection of glioma cell","authors":"Xiaohui Wang, Xinran Li, Baoquan Wang, Yanxia Xie, Xueru Cui, Jin Jiao, Jianwei Jiao, Yachao Zhang","doi":"10.1016/j.aca.2024.343555","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343555","url":null,"abstract":"<h3>Background</h3>Glioma accounts for 80% of all malignant primary brain tumors with a high mortality rate. Histopathological examination is the current diagnostic methods for glioma, but its invasive surgical interventions can cause cerebral edema or impair neural functioning. Liquid biopsy proves to be an efficient method for glioma detection. However, the blood-brain barrier restricts the number of circulating tumor cells (CTCs) in the bloodstream, posing a challenge for sensitive detection of glioma CTCs. This study aims to use the unique characteristics of nanocomposites and the specificity of Angiopep2 (Ang-2) to develop a method that can sensitively identify glioma CTCs.<h3>Results</h3>Herein, a novel label-free impedimetric biosensor was successfully constructed for glioma CTCs detection by using graphene quantum dots (GQDs)-nanoporous gold (NPG) nanocomposites as the immobilized platform and the Ang-2 protein as biorecognition element. The GQDs was homogeneously assembled onto NPG, resulting in the creation of a novel GQDs-NPG nanocomposite with unique structure and function properties. Due to the high electron transfer efficiency of the GQDs-NPG nanocomposite, the developed biosensor exhibited a wild detection range from 1 to 1×10⁶ cell mL^-1, with a minimal detection limit of 1 cell mL^-1. Additionally, the glioma cell biosensor demonstrated a strong anti-interference ability against multiple cell lines, and the stability of the biosensor remained at 96% after 21 days of storage. Besides, the quantities of glioma cells detected in human serum samples by the glioma cell biosensor demonstrated outstanding consistency with the standard values added to the samples.<h3>Significance</h3>The study provided a novel GQDs-NPG nanocomposite and an electrochemical biosensor based on GQDs-NPG was firstly developed for glioma CTCs detection. The glioma cell biosensor showed high sensitivity, low detection limit, strong anti-interference ability, and good stability in complex biological matrix. The reliable detection of glioma cell was successfully realized in human serum, providing an excellent option for liquid biopsy of glioma CTCs identification and early diagnosis of glioma diseases.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"46 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-powered photoelectrochemical sensor based on molecularly imprinted polymer-coupled CBFO photocathode and Ag2S/SnS2 photoanode for ultrasensitive dimethoate sensing","authors":"Shuqin Chen, Wanfu Lan, Dapeng Yang, Jingying Xu, Yikun Hu, Hetong Lin, Liang Feng","doi":"10.1016/j.aca.2024.343556","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343556","url":null,"abstract":"Dimethoate (DIM) is one of the most extensively applied organophosphorus pesticides (OPs), which is used to boost farm productivity due to its high insecticidal efficacy. However, the excessive use of DIM can result in the extensive contamination of soil, groundwater and food. Monitoring of DIM in environmental and food samples is crucial in view of its potential health risks and environmental hazards from excessive residues. The expensive equipment and complex operations for current detection methods greatly limit their practical applications. Herein, a self-powered photoelectrochemical (PEC) sensing platform based on Ag₂S/SnS₂ photoanode, iron-doped cobalt borate (CBFO) photocathode, and molecularly imprinted polymers (MIPs) was proposed for the detection of DIM. The molecularly imprinted polymers at CBFO photocathode endow the self-powered PEC sensor with high selectivity. The Ag₂S/SnS₂ photoanode enhances the efficient of electron transfer between the photoanode and photocathode, contributing to the high sensitivity of PEC sensor. The self-powered molecularly imprinted PEC sensor exhibits outstanding sensitivity and selectivity for DIM at concentrations from 1×10^-2 to 1×10⁵ nM with a detection limit of 5.9 pM. Excellent recoveries (95.4±2.6%, 98.4±2.3%, 106.3±3.3%) were achieved in spiked crown pear samples, indicating that the molecularly imprinted PEC sensor is capable of detecting DIM in real samples. This research provides a novel simple, fast, highly selective and sensitive self-powered molecularly imprinted photoelectrochemical sensing platform for detection of DIM. The fabricated PEC sensor offers a promising candidate for the detection method of organophosphorus pesticides residues, which is of great significance for the fields of food safety and environmental protection.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"244 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142832389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple and sensitive monitoring of polycyclic aromatic hydrocarbons in edible oils by polydimethylsiloxane/pyrazine-based hyper-crosslinked polymer coated stir bar sorptive extraction followed by gas chromatography-mass spectrometry detection","authors":"Yiwen Han, Aikun Han, Yaqiong Qin, Yuan Tian, Bin Peng, Lijun He, Wenfen Zhang, Wenjie Zhao, Shusheng Zhang","doi":"10.1016/j.aca.2024.343554","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343554","url":null,"abstract":"<h3>Background</h3>Edible oils are susceptible to contamination with polycyclic aromatic hydrocarbons (PAHs) throughout production, storage, and transportation processes due to their lipophilic nature. The necessity of quantifying PAHs present in complex oil matrices at trace levels, which bind strongly to impurities in oil matrices, poses a major challenge to the accurate quantification of these contaminants. Therefore, the development of straightforward and effective methods for the separation and enrichment of PAHs in oil samples prior to instrumental analysis is paramount to guaranteeing food safety.<h3>Results</h3>A pyrazine embedded hyper-crosslinked porous polymer, HCP_Pz-TPB, was synthesized via a Friedel-Crafts reaction, utilizing triphenylbenzene (TPB) as the monomer and 2,5-dibromomethylpyrazine as the cross-linking reagent. The material was combined with polydimethylsiloxane (PDMS) using the sol-gel method, and applied as a coating to a dumbbell-shaped stir bar prepared in-house. Using stir bar sorptive extraction (SBSE) combined with gas chromatography-mass spectrometry (GC-MS), 15 PAHs in edible oils were successfully quantified. Optimal conditions for the extraction of PAHs were experimentally investigated, with factors such as stirring rate, extraction time, extraction temperature, desorption solvent, and desorption time systematically optimized. The final method demonstrated a broad linear range (0.6–150 ng g^-1), and low limits of detection (0.04–0.28 ng g^-1). The recoveries of PAHs in real edible oil samples ranged from 83.14% to 128.01%, with relative standard deviations (RSDs) below 13.47%.<h3>Significance</h3>This method simplifies PAH extraction by eliminating steps such as saponification, liquid-liquid extraction, drying, and re-dissolution, thus reducing potential analyte loss and errors associated with the inclusion of multiple pretreatment steps typical of conventional methods reported in the literature. Notably, the adsorbent materials prepared in this study can be reused up to 30 times, underscoring its sustainability. The proposed research broadens the diversity of coating choices for SBSE applications while offering a streamlined, cost-effective, and greener alternative for PAH determinations in edible oils via SBSE/GC-MS.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"350 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel 3D-Printed Sample Preparation Method for Benzodiazepine Quantification in Human Serum","authors":"Dagmara Kroll, Szymon Ulenberg, Paweł Georgiev, Bartosz Marciniak, Gert Desmet, Tomasz Bączek, Mariusz Belka","doi":"10.1016/j.aca.2024.343552","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343552","url":null,"abstract":"<h3>Background</h3>Benzodiazepine abuse remains a significant public health concern. Current sample preparation methods for benzodiazepine analysis from human serum often involve complex procedures that require large sample volumes and extensive organic solvent use. To address these limitations, this study presents a novel and efficient sample preparation method utilizing 3D-printed sorbent devices.<h3>Results</h3>The 3D-printed devices, fabricated from a thermoplastic composite incorporating C18-modified silica, demonstrated exceptional performance in extracting benzodiazepines from human serum. The method was optimized and validated according to ICH guidelines, ensuring its reliability for quantitative benzodiazepine analysis. Notably, the method required minimal sample and solvent volumes, eliminating the need for protein precipitation, evaporation, and reconstitution.<h3>Significance</h3>This novel sample preparation approach offers significant advantages over traditional methods, providing a more efficient and environmentally friendly solution for benzodiazepine analysis. The versatility of 3D printing allows for the customization of sorbent devices for various analytes and matrices, expanding the potential applications of this method. Coupled with a rapid and robust LC-MS method optimized with DryLab, this approach presents a valuable and sensitive tool for benzodiazepine monitoring in clinical and toxicological settings.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"10 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging opportunities for intact and native protein analysis using chemical proteomics","authors":"Alexis N. Edwards, Ku-Lung Hsu","doi":"10.1016/j.aca.2024.343551","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343551","url":null,"abstract":"Chemical proteomics has advanced small molecule ligand discovery by providing insights into protein-ligand binding mechanism and enabling medicinal chemistry optimization of protein selectivity on a global scale. Mass spectrometry is the predominant analytical method for chemoproteomics, and various approaches have been deployed to investigate and target a rapidly growing number of protein classes and biological systems. Two methods, intact mass analysis (IMA) and top-down proteomics (TDMS), have gained interest in recent years due to advancements in high resolution mass spectrometry instrumentation. Both methods apply mass spectrometry analysis at the proteoform level, as opposed to the peptide level of bottom-up proteomics (BUMS), thus addressing some of the challenges of protein inference and incomplete information on modification stoichiometry. This Review covers recent research progress utilizing MS-based proteomics methods, discussing in detail the capabilities and opportunities for improvement of each method. Further, heightened attention is given to IMA and TDMS, highlighting these methods’ strengths and considerations when utilized in chemoproteomic studies. Finally, we discuss the capabilities of native mass spectrometry (nMS) and how it can be used in chemoproteomics research to complement existing approaches to further advance the field of functional proteomics.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"66 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent developments on aerial lab-on-a-drone platforms for remote environmental monitoring: A review","authors":"Habdias A. Silva-Neto, Danielle da Silva Sousa, Lucas C. Duarte, João Flávio da Silveira Petruci, Wendell K.T. Coltro","doi":"10.1016/j.aca.2024.343544","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343544","url":null,"abstract":"<h3>Background</h3>Distinct classes of environmental contaminants – such as microplastics, volatile organic compounds, inorganic gases, hormones, pesticides/herbicides, and heavy metals – have been continuously released into the environment from different sources. Anthropogenic activities with unprecedented consequences have impacted soil, surface waters, and the atmosphere. In this scenario, developing sensing materials and analytical platforms for monitoring water and air quality is essential to supporting worldwide environmental control agencies. Moreover, the distribution of such chemicals in the environment is not uniform, which requires the development of mobile and versatile analytical platforms to characterize the spatial and temporal profile of the pollutant's dispersion.<h3>Results</h3>Drones are excellent alternatives for <em>in-field</em> and remote monitoring with onboard analytical platforms. This review presents and discusses critical aspects related to drone-based platforms – i.e. lab-on-a-drone – for sampling and/or quantifying analytes dedicated to environmental (i.e. water and air) applications. A practice guide including the price range, max payload, flying time, and commercially available models is also introduced to support researchers selecting drones. As analytical procedures, colorimetric and electrochemical devices are described as powerful analytical tools to be attached in drones and to perform different environmental analyses. Lastly, examples of applications involving direct and indirect analysis of distinct pollutants, including toxic gases, particulate matter, and organic and inorganic ones, are highlighted. To date, no other review addresses the critical aspects of recent developments over the past five years in sampling and detection systems embedded in drones for environmental applications.<h3>Significance and novelty</h3>This review provides a comprehensive overview of technological innovations and compares the features of leading drone models, highlighting critical aspects for implementing portable analytical systems for air and water sampling and chemical analysis. We hope this work offers a clear perspective on current trends and provides valuable practical guidance for researchers and professionals interested in conducting remote environmental analyses.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"9 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital recombinase polymerase amplification chip based on asymmetric contact angle composite interface","authors":"Zhongping Zhang, Tianwei Li, Yao Tan, Luyao Liu, Hao Chen, Lulu Zhang, Michael G. Mauk, Xianbo Qiu","doi":"10.1016/j.aca.2024.343543","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343543","url":null,"abstract":"<h3>Background</h3>Digital recombinase polymerase amplification (dRPA) is an effective tool for the absolute quantification of nucleic acids and the detection of rare mutations. Due to the high viscosity or other physical properties of the reagent, this can compromise the accuracy and reproducibility of detection results, which limits the broader adoption and practical application of this technology. In this study, we developed an asymmetric contact angle digital isothermal detection (ACA-DID) chip and optimized the ACA-DID chip structure to achieve rapid digital recombinase polymerase amplification.<h3>Result</h3>We designed with a pressure-driven and highly asymmetric contact angle composite interface to enable robust digital RPA. The addition of surfactants to the PDMS creates an asymmetric contact angle between the upper and lower surfaces of the fluid channel, improving reagent flow and facilitating entry into microwells. This design addresses the challenges posed by high-viscosity reagents, which typically complicate effective digital discretization and lead to fluorescence signal aggregation. By diluting specific components of the RPA reagent, we improved the uniformity of amplification and effectively reduced signal aggregation. The hydrophobic surface of results strong adsorption to biological macromolecules, such as nucleic acids and proteins, which will decrease the efficiency of dRPA amplification. To achieve efficient amplification of reagents in the microchamber, this work uses a surface modification strategy of PDMS doped surfactant, which eliminates the issue of PDMS materials hindering dRPA amplification efficiency.<h3>Significance and novelty</h3>The ACA-DID chip demonstrated excellent analytical accuracy in the quantification of African swine fever DNA samples, highlighting its potential to enhance the accessibility and effectiveness of dRPA technology. This innovation promises to overcome key limitations in current digital RPA amplification platforms, driving broader adoption and application, especially in cost-sensitive or resource-limited settings.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"43 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biarsenical-based fluorescent labeling of metallothioneins as a method for ultrasensitive quantification of poly-Cys targets","authors":"Adam Pomorski, Sylwia Wu, Michał Tracz, Alicja Orzeł, Jaroslava Bezdekova, Agata Brambor, Aleksandra Suszyńska, Katarzyna Piekarowicz, Markéta Vaculovičová, Artur Krężel","doi":"10.1016/j.aca.2024.343550","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343550","url":null,"abstract":"<h3>Background</h3>Mammalian metallothioneins (MTs) play a crucial role in maintaining Zn(II) and Cu(I) homeostasis, as well as regulating the cellular redox potential. They are involved in cancer resistance to cisplatin-related drugs and the sequestration of toxic metal ions. To investigate their participation in specific physiological and pathological processes, it is imperative to develop an analytical method for measuring changes in protein concentration both <em>in vitro</em> and <em>in vivo</em>. Over the years, numerous methods have been developed for this purpose; however, they often suffer from issues such as low sensitivity, lengthy sample preparation, or variability in antibody batches.<h3>Results</h3>In this study, we present, for the first time, the application of the fluorescent biarsenical probe CrAsH-EDT₂ for the specific and ultrasensitive detection of human MTs. The interaction between the small library of biarsenical probes and 11 human MT isoforms (MT1-MT4), isolated MT2 domains, snail LlMT, and plant phytochelatin PC4 was investigated using spectrofluorimetry and mass spectrometry. A key advantage of our method is that the probe excess does not need to be removed before measurement as unbound is virtually non-fluorescent. We established protocols for MT detection in various assays, including cuvette-based, SDS-PAGE, and capillary electrophoresis. A detection limit (LOD) of 100 fg for human MT2 was achieved. Finally, we observed increased metallothionein concentration in lysates of HeLa cells treated with ZnSO₄.<h3>Significance</h3>In conclusion, our results demonstrate that the established method is easy to apply, versatile, and can be adapted to detect metallothioneins and other poly-Cys targets in different assays and applications.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"9 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142820608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Wearable Electrochemical Sensor Utilizing Multifunctional Hydrogel for Antifouling Ascorbic Acid Quantification in Sweat","authors":"Zhen Wei, Yanxin Li, Shuyue Guo, Rui Han, Xiliang Luo","doi":"10.1016/j.aca.2024.343548","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343548","url":null,"abstract":"The accurate and reliable quantification of the levels of disease markers in human sweat is of significance for health monitoring through wearable sensing technology, but the sensors performed in real sweat always suffer from biofouling that cause performance degradation or even malfunction. We herein developed a wearable antifouling electrochemical sensor based on a novel multifunctional hydrogel for the detection of targets in sweat. The integration of polyethylene glycol (PEG) into the sulfobetaine methacrylate (SBMA) hydrogel results in a robust network structure characterized by abundant hydrophilic groups on its surface, significantly enhancing the PEG-SBMA hydrogel's antifouling and mechanical properties. The wearable sweat sensor was developed by is specifically designed for ascorbic acid (AA) detection. The incorporation of a silver nanoparticles-molybdenum disulfide (AgNPs-MoS₂) composite material into the hydrogel significantly enhances its catalytic properties towards AA. Electrochemical analysis confirms that the sensor reliably detects AA in real sweat with minimal interference from other components and bacteria, demonstrating its practical application potential. Furthermore, this multifunctional hydrogel's mechanical robustness and strong adhesion to various substrates ensure its practical applicability in wearable devices. This technology provides a foundation for accurate health monitoring in wearable sensors, enabling advanced, non-invasive diagnostic tools for personalized healthcare.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"23 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}