Analytica Chimica Acta最新文献

{"title":"An activatable “AIE + ESIPT” fluorescent probe for dual-imaging of lipid droplets and hydrogen peroxide in drug-induced liver injury model","authors":"Wantao Liao, Chunzheng Wang, Ruiya Wang, Mengzhao Wu, Lanqing Li, Pengjie Chao, Jinhui Hu, Wen-Hua Chen","doi":"10.1016/j.aca.2024.343442","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343442","url":null,"abstract":"<h3>Background</h3>Drug-induced liver injury (DILI) is one of the most common liver diseases. The crucial role of lipid droplets (LDs) and hydrogen peroxide (H₂O₂), two important biomarkers in the pathophysiology of DILI, has spurred considerable efforts to accurately visualize H₂O₂ and LDs for elucidating their functions in the progression of DILI. However, construction of a single fluorescent probe that is able to simultaneously image H₂O₂ and LDs dynamics remains to be a challenging task. Therefore, it is of great demand to develop a novel fluorescent probe for tracking the LDs status and H₂O₂ fluctuation in drug-induced liver injury.<h3>Results</h3>We developed an “AIE + ESIPT” fluorescent probe <strong>TPEHBT</strong> for dual-imaging of LDs and H₂O₂ during DILI process. <strong>TPEHBT</strong> displayed greatly enhanced fluorescent response to H₂O₂ by generating an excited state intramolecular proton transfer (ESIPT) fluorophore <strong>TPEHBT-OH</strong> with aggregation induced emission (AIE) properties. <strong>TPEHBT</strong> exhibits high selectivity, sensitivity (LOD = 4.73 nM) and large Stokes shift (320 nm) to H₂O₂. Interestingly, <strong>TPEHBT</strong> can light up LDs with high specificity. The probe was favorably applied in the detection of endogenous and exogenous H₂O₂ in living cells, and notably in the simultaneous real-time visualization of H₂O₂ generation and LDs accumulation during DILI process. Moreover, <strong>TPEHBT</strong> was able to image H₂O₂ generation in zebrafish animal model with APAP-induced liver injury.<h3>Significance</h3>For the first time, probe <strong>TPEHBT</strong> was applied in the dual-imaging of H₂O₂ fluctuation and LDs status in APAP-induced liver injury model <em>in vitro</em> and <em>in vivo</em>. The present findings strongly suggest that <strong>TPEHBT</strong> is a promising tool for monitoring H₂O₂ and LDs dynamics in DILI progression.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"18 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplex Digital PCR for the simultaneous quantification of a miRNA panel","authors":"Florence Busato, Sylvain Ursuegui, Jean-François Deleuze, Jorg Tost","doi":"10.1016/j.aca.2024.343440","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343440","url":null,"abstract":"<h3>Background</h3>microRNAs (miRNAs) are small non-coding RNAs regulating gene expression. They have attracted significant interest as biomarkers for early diagnosis, prediction and monitoring of treatment response in many diseases. As individual miRNAs often lack the required sensitivity and specificity, miRNA signatures are developed for clinical applications. Digital PCR (dPCR) is a sensitive fluorescent-based quantification method, that can be used to detect the expression of miRNAs in patient samples. Our study presents the first proof-of-concept of a multiplexed dPCR assay for the simultaneous analysis and quantification of multiple miRNAs.<h3>Results</h3>After reverse transcription (RT) using a pool of miRNA-specific stem-loop primers, dPCR was performed with a universal reverse primer and miRNA-specific forward primers along with fluorescently-labelled hydrolysis probes. Multiple experimental parameters were evaluated and strategies for modulating the observed signals were devised. The optimised assay was applied to the analysis of miRNAs from cell lines and biological samples. Although absolute quantification was lost, due to the reverse transcription step, quantification was linear for the dilution series and results were highly reproducible for independent dPCR and RT reactions. Our results confirmed the high sensitivity of dPCR for patient samples.<h3>Conclusions</h3>We demonstrate the feasibility and reliability of multiplexed detection and quantification of miRNAs by dPCR that can be applied in a clinical setting to evaluate miRNA signatures.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"99 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated self-powered sensors based on cubic nanostructure and cascade amplification strategies","authors":"Hanxiao Chen, Xinqi Luo, Yilin Liu, Kexin Guo, Jingying Han, Jing Xu","doi":"10.1016/j.aca.2024.343446","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343446","url":null,"abstract":"The advantages of simple structure and easy portability inherent to self-powered biosensors have broad application prospects in clinical diagnosis and implantable medical devices. In light of the limitations of the clinical detection sensitivity, this study devised and fabricated a three-dimensional (3D) cubic structure of MoS₂ that was self-assembled by 2D nanosheets. The multi-dimensional structure design provides a broad binding site for the incubation of biological probes and the loading of biological enzymes, which can accelerate the electron transfer rate and effectively improve the detection sensitivity. On this basis, a self-powered biosensor platform was constructed for the detection of miRNA-499, employing the catalytic hairpin self-assembly (CHA) signal amplification strategy. This strategy has the potential to enhance the accuracy of detection and circumvent the occurrence of false positive results. The experimental results demonstrate that the self-powered biosensor exhibits a detection limit (LOD) of 0.12 fmol/L (S/N = 3) within a wide linear range of 1 fmol/L to 100 pmol/L. Furthermore, the sensor platform exhibits excellent specificity, stability, and reproducibility. Moreover, the test signal is transmitted via Bluetooth to the smartphone interface, thus meeting the requirements for portable, real-time detection.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"53 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microparticle-Assisted Protein Capture Method Facilitates Proteomic and Glycoproteomic Analysis of Urine Samples","authors":"Zhongyu Wang, Zheng Fang, Zhenzhen Wang, Hongqiang Qin, Zhimou Guo, Xinmiao Liang, Shuxin Liu, Mingming Dong, Mingliang Ye","doi":"10.1016/j.aca.2024.343448","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343448","url":null,"abstract":"Serum tests have become a partial alternative to renal biopsy for diagnosing primary membranous nephropathy (pMN). However, urine tests, due to their non-invasive nature and ability to more accurately reflect glomerular diseases, hold great promise for the detection of pMN. However, the low protein concentration and the time-consuming sample preparation procedure of urine samples challenges the proteomic and glycoproteomic analysis to find urine-derived signatures associated with pMN. In this study, we presented a microparticle-assisted protein capture (MAPC) method to efficiently prepare urine samples. It was found that proteins and N-linked intact glycopeptides can be sensitively identified from urine samples of pMN patients and healthy controls by using this method. For comparison, proteins and N-linked intact glycopeptides from serum were also analyzed. Interestingly, discrepancy was found in the changing trends for proteins/intact glycopeptides between serum and urine. Moreover, urine derived proteins, N-linked intact glycopeptides, and glycans exhibited more pronounced changes between pMN and healthy control compared to serum sample. Notably, urine IgG4 not only up-regulated in pMN at global peptide level, its corresponding site-specific glycans were specifically identified in pMN urine with significantly up-regulations, suggested the potential of using glycosylated urine IgG4 for the non-invasive diagnosis of pMN.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"15 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of microcystins and nodularins in ambient freshwater and seawater by liquid chromatography- mass spectrometry including toxin screening and identification","authors":"Zhihong Wang, Christina M. Mikulski, Makayla Kent, Tod Leighfield, Gregory J. Doucette, John S. Ramsdell","doi":"10.1016/j.aca.2024.343449","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343449","url":null,"abstract":"<h3>Background</h3>Microcystins (MCs) and nodularins (NODs) produced by cyanobacteria occur in ambient freshwaters and across the freshwater-marine continuum, and pose health threats through drinking and recreational waters, as well as food resources. Approximately 300 MC and NOD toxins have been published, but less than 15 of them are commercially available as toxin standards. Our aim herein was to rapidly identify and quantify all toxin congeners, including those without standards, in water samples even at low abundance by reversed-phase solid phase extraction (SPE)-liquid chromatography-tandem mass spectrometry (LC-MS/MS) to provide insights into toxin levels and potential toxicity.<h3>Results</h3>Trizma instead of acid was used as an ion pairing reagent to increase retention of MCs without arginine residues on Strata X SPE. Toxin elution from Oasis HLB SPE was complicated by their hydrophilic and lipophilic interactions with HLB sorbent. Three stable isotope-labeled (SIL) toxin standards representing MCs carrying 2, 1, and 0 arginine residues served as internal standards (IS) for toxin determination and evaluation of cyanobacterial cell lysis methods. Average recoveries of toxin standards in lake water and seawater using the HLB sorbent for validation ranged from 90 to 109% except MC-WR &amp; LW (71 to 87%) with detection limits from 1.3 to 23.7 ng L^-1. Doubly charged protonated toxin molecular ions were employed as precursors for tandem MS to screen and identify toxin congeners using a hybrid triple quadrupole linear ion trap MS. More than 30 (4 new) MCs were detected in <em>Microcystis aeruginosa</em> strain LE-3 culture.<h3>Significance</h3>This is the first report to explain the issues with and possible mechanisms for SPE extraction of MCs from water, to use SIL-IS to evaluate methods for lysing cyanobacterial cells for MC release, and to show that doubly rather than singly charged toxin molecular ions as MS/MS precursors enabled efficient screening, identification, and quantification of all toxins at low levels. A MC with homoalanine residue at position 1 was reported for the first time.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"14 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-switch electrochemical aptasensor for label-free detection of thrombin and ATP based on split aptamers","authors":"Mengran Song, Xiaowei Wu, Kaiyan Fan, Guanxia Qiu, Xiuhua Zhang, Zhen Wu, Shengfu Wang, Wei Wen","doi":"10.1016/j.aca.2024.343441","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343441","url":null,"abstract":"<h3>Background</h3>Aptamers, consisting of specialized single-stranded nucleic acids, are engineered through the SELEX technique to recognize specific targets with strong affinity. Aptamers are exceptionally useful in various sensor technologies, such as fluorescence-based sensors, electrochemical sensors, and colorimetric detection systems. Due to its high sensitivity, specificity and fast response, electrochemical aptasensor shows great application prospects in analytical detection, food safety, and environmental monitoring. However, one aptasensor can usually detect only one type of target, limiting its universality in practical applications.<h3>Results</h3>Here, we constructed a dual-switch and label-free electrochemical aptasensor based on split aptamer and nuclease. The feasibility, specificity, and sensitivity of the aptasensor were investigated by using thrombin and adenosine triphosphate (ATP) as targets. Split aptamer can not only capture target specifically but also form a stable sandwich structure with the target. In the presence of thrombin, it triggered a hydrolysis reaction of exonuclease I, leading to a decrease in the impedance signal. Differently, the presence of ATP could form a sandwich structure with split aptamers, leading to an increase in output signals. The aptasensor achieved sensitive and specific detection of thrombin and ATP, with low detection limits of 0.76 pM and 0.27 pM, respectively.<h3>Significance and Novelty</h3>The aptasensor realized the detection of two targets without replacing any reagents or equipment, which greatly saved time and cost. Furthermore, electrochemical impedance spectroscopy (EIS) uses impedance as an output signal, showing great application prospects in electrochemical aptasensors as label-free and simple methods. Because of its simplicity, label-free, and sensitivity in complex samples, the split aptamer-assisted aptasensor provides new ideas and methods in early diagnosis of diseases.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"53 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequencing the Monoclonal Antibody Variable Regions Using Multiple Charge Integration Middle-Down Strategy and Ultraviolet Photodissociation","authors":"Jialiang Liu, Zheyi Liu, Heng Zhao, Chunlei Xiao, Xueming Yang, Fangjun Wang","doi":"10.1016/j.aca.2024.343450","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343450","url":null,"abstract":"<h3>Background</h3>Therapeutic monoclonal antibodies (mAbs) have become essential biopharmaceuticals for clinical targeted therapies due to their high specificity, affinity and low side effects. The specificity and affinity of mAb to targeting antigen are mainly dependent on the three complementarity determining regions (CDRs) with high variations in amino acid sequences. Therefore, mAb CDR sequencing is crucial for the characterization of therapeutic mAbs. Here, we developed a 193-nm ultraviolet photodissociation (UVPD) based multiple charge integration middle-down mass spectrometry (MCI-MDMS) strategy for mAb sequencing.<h3>Results</h3>We demonstrate that the UVPD spectra of mAb subunit ions with different charge states exhibit high complementarity, and integration can result in higher sequence coverage compared to single charge states. Finally, over 95% sequence coverage of two different mAbs has been achieved with full sequence coverage of CDRs, underscoring the great potential of this strategy in accurate sequencing of mAb variable regions. Compared with the conventional higher energy collisional dissociation (HCD) strategy of mAb subunit sequencing, the sequence coverage of CDRs at single UVPD subunit charge state has increased by an average of 30%. In addition, almost complete sequence coverage of mAb ensures the accurate localization of mAb post-translational modifications (PTMs), including glycosylation of two different sites, C-terminal lysine truncation, and N-terminal cyclization of glutamine.<h3>Significance</h3>The integration of MCI-MDMS and UVPD realizes high sequence coverage and reliable PTM determination of mAbs. This integrated strategy holds significant promise for accurate analysis of antibody-drug conjugates, polyclonal antibodies and unknown mAbs including sequences and PTMs, and providing a crucial tool for the discovery and development of therapeutic mAbs.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"36 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photo-controlled cascade DNA hybridization for amplified electrochemical biosensor with tunable sensing performance","authors":"Fangfang Yang, Shuang Li, Xiaolin Zhang, Shufeng Liu","doi":"10.1016/j.aca.2024.343447","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343447","url":null,"abstract":"<h3>Background</h3>Precise control of the biorecognition process in DNA biosensors, especially for those with signal amplification, remains a challenge. It is of great significance to introduce external stimuli into the DNA system for a controllable trigger of nucleic acid cascade amplification and further for excellent biosensors.<h3>Results</h3>In this study, a photo-initiated hybridization chain reaction (HCR) was designed for controllable and sensitive electrochemical biosensor via the incorporation of azobenzene moiety into the assembly unit. Under the coexistence of UV light and target DNA, a number of HCR products with biotin tags were generated and fixed on the electrode surface. Subsequently, the bound streptavidin-labeled horseradish peroxidase (SA-HRP) effectively catalyzed H₂O₂-mediated oxidation of tetramethylbenzidine (TMB), producing significant electrochemical current signals. A tunable sensing performance with different dynamic response ranges and sensitivity was achieved by adjusting the number of the inserted azobenzene moieties and the control of UV light. A limit of detection as low as 2.5 fM (S/N=3) could be obtained in the case of one azobenzene and under UV exposure. Moreover, the photo-controlled DNA biosensor exhibited good discrimination ability even against single-base mismatch and was able to be applied in serum samples.<h3>Significance</h3>The proposed electrochemical DNA biosensor based on dual-triggered HCR amplification may represent a promising path to achieve sensitive and accurate bioanalysis. Also, the tunable dynamic range of the developed biosensor will provide the possibility of clinical applications.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"10 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peroxynitrite activatable NIR-II probe for tumor diagnosis and photothermal therapy in vivo","authors":"Muxin Xu, Yanyan Ma, Yuping Zhao, Lizhen Xu, Weiying Lin","doi":"10.1016/j.aca.2024.343443","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343443","url":null,"abstract":"<h3>Background</h3>Peroxynitrite (ONOO^-) is a bioactive molecule involved in various biochemical processes, and the abnormal concentration fluctuations of ONOO^- in living systems are closely associated with various diseases, including cancer. An important characteristic of the tumor microenvironment is the overexpression of ONOO^-, highlighting the significance of specific detection of ONOO^- in distinguishing between tumor tissue and normal tissue. A single near-infrared second window (NIR-II) molecular probe integrated fluorescence imaging and photothermal therapy can achieve precise localization and effective ablation of deep-seated tumor tissue. However, it still remains challenges. (87)<h3>Results</h3>In this study, we present a probe (<strong>BDⅡ-ONOO</strong>^{<strong>-</strong>}) that integrates NIR-II fluorescence imaging and photothermal therapy for the specific detection of ONOO^-. The probe exhibits excellent selectivity, high sensitivity, low toxicity and high biocompatibility. In the presence of ONOO^-, the probe can quickly respond to ONOO^- and emit NIR-II fluorescence at 900 nm with 7.6-fold change in fluorescence intensity. In addition, the probe <strong>BDⅡ-ONOO</strong>^{<strong>-</strong>} exhibits a high photothermal conversion efficiency of 41.6% under 808 nm laser irradiation in the presence of ONOO^-. In vivo imaging results indicate that the probe <strong>BDⅡ-ONOO</strong>^{<strong>-</strong>} not only effectively distinguishes tumor tissue from normal tissue but also performs photothermal treatment on 4T1 tumor without apparent biological toxicity. (112)<h3>Significance</h3>This work provides insights for the future development of tumor-specific diagnostic and therapeutic approaches with good biocompatibility and deep tissue penetration. The probe described in this work may be employed as a powerful tool for the diagnosis and precise treatment of tumors <em>in vivo</em>. (44)","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"99 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphene oxide-based fluorescent biosensors for pathogenic bacteria detection: a review","authors":"Shiwu Liu, Fangguo Lu, Shanquan Chen, Yi Ning","doi":"10.1016/j.aca.2024.343428","DOIUrl":"https://doi.org/10.1016/j.aca.2024.343428","url":null,"abstract":"<h3>Background</h3>Pathogenic bacteria are widespread in nature and can cause infections and various complications, thereby posing a severe risk to public health. Therefore, simple, rapid, sensitive, and cost-effective methods must be developed to detect pathogenic bacteria. Biosensors are prominent platforms for detecting pathogenic bacteria owing to their high sensitivity, specificity, repeatability, and stability. With the development of nanotechnology, graphene oxide (GO) has been increasingly introduced into the construction of fluorescent biosensors to enhance their performance owing to its unique physicochemical properties.<h3>Results</h3>This review systematically summarizes the development of GO-based fluorescent biosensors for the detection of pathogenic bacteria. First, we introduce the functionalization and modification of GO. The design and signal amplification strategies for GO-based fluorescent biosensors are also discussed. Finally, we explore the challenges and new perspectives associated with this field, with the aim of facilitating the development of GO-based fluorescent sensing technologies to prevent the spread of multidrug-resistant bacteria.<h3>Significance</h3>This review will aid in the development of high-performance biosensors for pathogenic bacterial assays.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"10 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}