Analytica Chimica Acta最新文献

{"title":"Preparation of ZIF-8 grafted magnetic solid-phase extraction sorbent for sensitive determination of four cannabinoids in urine and oral fluid","authors":"Hongyu Ning ,&nbsp;Yilei Fan ,&nbsp;Haodong Wang ,&nbsp;Huijun Liu ,&nbsp;Zhongping Huang ,&nbsp;Haixing Wang ,&nbsp;Xing Ke ,&nbsp;Yu Xu","doi":"10.1016/j.aca.2026.345194","DOIUrl":"10.1016/j.aca.2026.345194","url":null,"abstract":"<div><h3>Background</h3><div>The global increase in cannabis use has created a strong demand for reliable and efficient detection methods. Traditional urine-based assays are widely used and standardized, but suffer from complex hydrolysis requirements, long detection windows, and an inability to monitor recent use. In contrast, oral fluid testing enables non-invasive, real-time detection of parent cannabinoids, but is limited by low sample volume, matrix interference, and insufficient sensitivity in common rapid tests. It is clear that a sample preparation and detection approach is needed that is robust, highly sensitive, streamlined, and compatible with high-throughput confirmatory analysis such as LC-MS/MS.</div></div><div><h3>Results</h3><div>In this study, a magnetic solid-phase extraction (MSPE) method was developed using a novel magnetic metal–organic framework composite, Fe₃O₄@poly(GMA/DVB-ZIF-8), for the efficient extraction of four cannabinoids from urine and oral fluid. The sorbent uses multiple interactions—including hydrophobic, π–π, electrostatic, and Zn–O coordination—to achieve high analyte recovery (exceeding 85%). Coupled with UHPLC-MS/MS analysis, the method demonstrates wide linearity (0.05–25 ng/mL, r² &gt; 0.9945), low detection limits (as low as 0.017 ng/mL), and good repeatability (RSDs &lt;13.8%). Comparative evaluation with conventional liquid–liquid extraction showed minimal deviation (relative percent difference &lt;6.0%), confirming high trueness and reliability for cannabinoid monitoring in biological samples.</div></div><div><h3>Significance</h3><div>This MSPE approach provides a simple, efficient and high-throughput sample preparation method for reliable and rapid forensic analysis of cannabinoids in complex biological samples.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1394 ","pages":"Article 345194"},"PeriodicalIF":6.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146153231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative all-in-one high-performance thin-layer chromatography–planar start-zone enzyme assay applied for invertase activity","authors":"Isabel Müller,&nbsp;Viktoria H. Englert,&nbsp;Gertrud E. Morlock","doi":"10.1016/j.aca.2026.345213","DOIUrl":"10.1016/j.aca.2026.345213","url":null,"abstract":"<div><h3>Background</h3><div>Conventional methods for assessing enzyme activity often rely on <em>in vitro</em> assays with spectrophotometric detection; however, they lack accuracy for complex substrates. The recently introduced nanoGIT concept demonstrated the potential of on-surface enzyme assays performed directly in the start zone, followed by high-performance thin-layer chromatography (HPTLC) of the formed products on the same adsorbent surface. For the first time, the potential of this all-in-one HPTLC concept was investigated for the quantification of enzyme (invertase) activity using a complex substrate.</div></div><div><h3>Results</h3><div>Enzymatic hydrolysis of sucrose or egg biscuit extract was carried out in the start zone under identical conditions for all studied samples. On the same adsorbent surface, the hydrolysis products were separated, detected via densitometric fluorescence measurement, and quantified. Even with complex substrates, the integrated separation provided accurate, in-depth information on enzyme characteristics from the individual products formed and their respective intensities. The method was validated and proved to be accurate and reliable in the nanomolar range, outperforming current spectrophotometric <em>in vitro</em> assays that operate in the millimolar range. The assay in the start zone exhibited remarkable sensitivity, requiring a 12,500-fold lower substrate quantity while using equivalent enzyme amounts. Limitations and benefits were discussed.</div></div><div><h3>Significance</h3><div>For the first time, a validated quantitative all-in-one HPTLC–start-zone enzyme assay–FLD method was successfully demonstrated for the determination of the enzyme activity. It enabled the quantification of even minor amounts of individual enzyme reaction products, providing improved sensitivity and more accurate insights into enzyme characteristics. Low enzyme levels can be analysed, extending current analytical capabilities.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1394 ","pages":"Article 345213"},"PeriodicalIF":6.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146138739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardized digital PCR assay validation using PCR-ValiPal, demonstrated in cross-platform quantification of bovine papilloma virus","authors":"David Gleerup ,&nbsp;Matthijs Vynck ,&nbsp;Lien Gysens ,&nbsp;Cindy De Baere ,&nbsp;Wim Trypsteen ,&nbsp;Jo Vandesompele ,&nbsp;Olivier Thas ,&nbsp;Ann Martens ,&nbsp;Maarten Haspeslagh ,&nbsp;Ward De Spiegelaere","doi":"10.1016/j.aca.2026.345210","DOIUrl":"10.1016/j.aca.2026.345210","url":null,"abstract":"<div><h3>Background</h3><div>Digital PCR (dPCR) enables precise and absolute quantification of nucleic acids by partitioning samples into thousands of reactions, improving reproducibility and reducing reliance on standard curves compared to qPCR. However, rigorous assay validation remains essential to ensure reliability, particularly for parameters such as limit of detection, limit of quantification, trueness, and linearity. Existing guidelines (e.g., MIQE, dMIQE, ISO 20395:2019) highlight these requirements, but implementation is laborious and inconsistent across laboratories. To address this, we developed PCR-ValiPal, a user-friendly web application that standardizes and streamlines dPCR assay validation and reporting.</div></div><div><h3>Results</h3><div>PCR-ValiPal calculates the full range of analytical parameters required for ISO-compliant assay validation, including limit of blank, limit of detection, limit of quantification, precision, trueness, and linearity. While broadly applicable to any nucleic acid target, we demonstrate its use with a three-color PCR assay for bovine papillomavirus (BPV), a clinically relevant representative DNA assay, types 1 and 2, benchmarked across four platforms: Naica (droplet dPCR), QIAcuity (microwell dPCR), LOAA (real-time dPCR), and CFX96 (qPCR). Cross-platform comparisons revealed Naica and QIAcuity achieved low LOB and LOQ values with minimal bias, while LOAA exhibited stable but negative bias. qPCR performed best for BPV-2 sensitivity but was less reliable for BPV-1 at low concentrations. These results illustrate both the value of platform-specific optimization and the utility of PCR-ValiPal in providing transparent, standardized validation outputs.</div></div><div><h3>Significance</h3><div>PCR-ValiPal supports transparent, reproducible, and ISO-aligned validation of PCR-based assays, lowering barriers for both expert and non-expert users. By centralizing statistical analyses in a single tool, it enables reliable comparison across platforms and targets, facilitating adoption in research, diagnostics, and regulatory contexts. This work underscores the importance of standardized validation for ensuring confidence in nucleic acid quantification.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1394 ","pages":"Article 345210"},"PeriodicalIF":6.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-dimensional AEX – IP-RPLC: New insights on mRNA integrity and encapsulation efficiency of mRNA-lipid nanoparticle formulations","authors":"Megane K. Aebischer ,&nbsp;Athanasios Tsalmpouris ,&nbsp;Serge Rudaz ,&nbsp;Camille Malburet ,&nbsp;Jean-François Cotte ,&nbsp;Davy Guillarme ,&nbsp;Jonathan Maurer","doi":"10.1016/j.aca.2026.345215","DOIUrl":"10.1016/j.aca.2026.345215","url":null,"abstract":"<div><h3>Background</h3><div>Therapeutics and vaccines involving mRNA have shown significant progress over the last five years. These lipid nanoparticle-based formulations introduce substantial analytical complexity, as multiple Quality Attributes (QAs) must be monitored to ensure product efficacy, stability, and safety. Encapsulation efficiency (EE) and mRNA integrity are particularly critical, yet current analytical approaches often require separate assays, challenging sample handling, and limited selectivity toward free versus encapsulated species. Therefore, new chromatographic methods capable of resolving these species and supporting multi-payload formulations remain a major unmet need.</div></div><div><h3>Results</h3><div>To rapidly obtain multiple QAs for mRNA-LNP formulations, we present an optimized two-dimensional liquid chromatography (2D-LC) workflow combining anion-exchange chromatography (AEX) in the first dimension (¹D) and ion-pair reversed-phase liquid chromatography (IP-RPLC) in the second dimension (²D). The ¹D-AEX evaluated encapsulation efficiency of mRNA within lipid nanoparticles based on the mRNA ratio of intact and disrupted drug products, while ²D-IP-RPLC assessed integrity profiles and mRNA-lipid adducts. Attention was paid to the ²D optimization to eliminate solvent incompatibilities and ensure efficient transfer between dimensions.</div><div>This approach enables simultaneous determination of EE, mRNA integrity, mRNA-lipid adducts, and transcript ratios in multi-payload mRNA-LNP formulations. It also provides chromatographic assessment of free mRNA integrity within formulations. This method furthermore enables the characterization of additional species and confirms the presence of surface-associated mRNA. The workflow demonstrates good selectivity and applicability to both mono- and multi-payload mRNA-LNP products.</div></div><div><h3>Significance</h3><div>Overall, the developed 2D-LC platform is a powerful analytical tool for comprehensive mRNA-LNP characterization. By enabling simultaneous assessment of several critical QAs, including EE, integrity, transcript ratios, and mRNA-lipid adducts, it streamlines analytical workflows and reduces reliance on multiple independent assays. This approach provides mechanistic insight into LNP structure, including detection of surface-associated RNA species, and establishes an innovative tool to support formulation development, process optimization, drug products release, and stability studies for next-generation mRNA vaccines and therapeutics.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1394 ","pages":"Article 345215"},"PeriodicalIF":6.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SPARC: A programmable molecular diagnostic platform based on a signal-triggered, self-supplied crRNA and tiered PER-transcription-CRISPR cascade for early detection of hepatocellular carcinoma","authors":"Xinying Chang ,&nbsp;Cong Han ,&nbsp;Haishuo Ji ,&nbsp;Zhe Zeng ,&nbsp;Jingyi Yang ,&nbsp;Qirui Liu ,&nbsp;Chao Jia ,&nbsp;Lili Zhao ,&nbsp;Chunlei Zhou ,&nbsp;Shujia Chen ,&nbsp;Wolfgang Knoll ,&nbsp;Jia Li ,&nbsp;Zhenglu Wang ,&nbsp;Liyun Zhang","doi":"10.1016/j.aca.2026.345209","DOIUrl":"10.1016/j.aca.2026.345209","url":null,"abstract":"<div><h3>Background</h3><div>Accurate quantification of microRNAs (miRNAs) is essential for early cancer detection, yet remains challenging due to their short length, low abundance, and high sequence similarity. Existing assays often struggle to achieve sufficient sensitivity, specificity, and robustness for reliable clinical deployment.</div></div><div><h3>Results</h3><div>We introduce <strong>SPARC</strong>, a programmable molecular diagnostic platform that integrates a signal-triggered primer exchange reaction, self-supplied crRNA generation, and a tiered PER–transcription–CRISPR/Cas12a amplification cascade. Using miRNA-21 as a model, SPARC achieves an ultralow detection limit of 1.22 fM and a broad quantitative range from 1 fM to 100 nM. The system exhibits high specificity, strong analytical stability, and modular adaptability to diverse targets, including miRNA-122. Notably, the dual-directional profiling of oncogenic and tumor-suppressive miRNAs enhances diagnostic resolution. When applied to HCC cell lines and clinical tissues, SPARC accurately distinguished malignant from normal samples and showed excellent agreement with qRT-PCR measurements and histopathological assessments.</div></div><div><h3>Significance</h3><div>This streamlined and self-amplifying cascade system provides a scalable, robust, and clinically compatible platform for ultrasensitive miRNA detection. SPARC holds strong potential for early hepatocellular carcinoma screening, molecular subtyping, and broader precision oncology applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1394 ","pages":"Article 345209"},"PeriodicalIF":6.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COF-Zincon dual signal amplification: A sensitive real-time electrochemical sensor for Zn2+ detection towards anxiety disorder diagnosis","authors":"Honglong Zou ,&nbsp;Yonggui Song ,&nbsp;Jiaqi Shao ,&nbsp;Bin Lai ,&nbsp;Qinghui Fu ,&nbsp;Zhifu Ai ,&nbsp;Yali Liu ,&nbsp;Yuhui Ping ,&nbsp;Dan Su ,&nbsp;Youhui Zhang ,&nbsp;Huihui Liang","doi":"10.1016/j.aca.2026.345220","DOIUrl":"10.1016/j.aca.2026.345220","url":null,"abstract":"<div><h3>Background</h3><div>In recent years, abnormal total zinc levels in serum have emerged as a robust indicator of anxiety disorder. However, measuring total zinc level requires sophisticated instrumentation and specialized personnel. Therefore, if the specific association between Zn²⁺ in serum and anxiety disorder can be elucidated, it is promising to develop rapid and convenient diagnostic methods for anxiety disorder. In the field of Zn²⁺ detection, Electrochemical detection has been investigated as a means to quantify Zn²⁺ with precision, rapidity and cost-effectiveness. Nevertheless, most current electrochemical systems lack specificity.</div></div><div><h3>Results</h3><div>In this study, the relationship between anxiety disorder and serum Zn²⁺ level was first elucidated, with research finding indicating that anxiety in mice correlated with a marked decline in serum Zn²⁺ level. Subsequently, a sensitive and specific electrochemical sensor was developed to detect Zn²⁺ with outstanding detection performance for the diagnosis of anxiety disorder. In this sensor, covalent organic framework material (COF_Tp-Th) was utilized to load a substantial amount of gold nanoparticles (AuNPs) via Au–N bonds to obtain COF_Tp-Th-AuNPs material. AuNPs can enhance electrode catalysis and conductivity and served as a platform for subsequent material integration. Furthermore, a large quantity of Zincon reagent for specific recognition and detection of Zn²⁺ was loaded onto the AuNPs through Au–S and Au–N bonds to improve electrode specificity, resulting in the construction of the target electrode modified material of COF_Tp-Th-AuNPs-Zincon.</div></div><div><h3>Significance</h3><div>Long-range ordered COF_Tp-Th can adsorb Zn²⁺ through its rich tetradentate coordination ONNO chelation sites formed by Tp and Th, representing the primary signal amplification. Simultaneously, the abundant Zincon in COF_Tp-Th-AuNPs-Zincon material with high affinity for the specific recognition and detection of Zn²⁺ contributed to secondary signal amplification. This approach leads to a significant improvement in the detection performance of Zn²⁺ and holds promise for providing robust support for the diagnosis and monitoring of anxiety disorder.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1394 ","pages":"Article 345220"},"PeriodicalIF":6.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendritic mixed-mode stationary phases prepared by thiol-epoxy click reaction for the determination of bisphenols in a variety of complex samples","authors":"Wuji Shuoti,&nbsp;Ruihan Peng,&nbsp;Xuemei Wang,&nbsp;Qiuxia Liu,&nbsp;Yixin Huang,&nbsp;Lishuo Liu,&nbsp;Yanyu Jiang,&nbsp;Jie Wen,&nbsp;Lian Zhong,&nbsp;Lujun Wang","doi":"10.1016/j.aca.2026.345216","DOIUrl":"10.1016/j.aca.2026.345216","url":null,"abstract":"<div><h3>Background</h3><div>Bisphenols are widely used in thermal paper, inks, coatings, and other fields. Among them, abnormal concentrations of bisphenol A (BPA) can disrupt the human endocrine system, making it essential to closely monitor BPA levels in environmental and daily life samples. Owing to its strong separation and analytical capabilities, high performance liquid chromatography (HPLC) enables accurate analysis of compounds in complex real-world samples. Among various HPLC stationary phases, dendritic stationary phase materials have attracted significant attention due to their unique branched spatial structure. Therefore, it is of great research importance to continuously investigate dendritic HPLC stationary phases, expand their variety, and broaden their applications.</div></div><div><h3>Results</h3><div>The novel dendrimer based stationary phase named as Sil-G1-BDDE-TTCA was synthesized by modifying 1,4-butanediol diglycidyl ether and trithiocyanuric acid onto silica surfaces via thiol-epoxy click reaction. Through three repeated grafting, the third-generation stationary phase named as Sil-G3-BDDE-TTCA was prepared. The C18 modified dendrimer based stationary phase named as Sil-G3-BDDE-TTCA-C18 was finally prepared using 1-octadecene as a capping functional monomer. These dendritic stationary phases were characterized by Elemental analysis, Thermogravimetric analysis, Scanning electron microscope and X-ray photoelectron spectroscopy. To evaluate the hydrophobic, hydrophilic, and <em>π-π</em> interactions of the prepared dendritic stationary phases, test mixtures including alkylbenzenes, positional isomers, polycyclic aromatic hydrocarbons, nucleosides, and flavonoids were analyzed. The Tanaka test was employed to compare the chromatographic performance of different stationary phases. Thermodynamic parameters for the retention of alkylbenzenes and positional isomers on these stationary phases were calculated to investigate the effect of temperature on chromatographic behavior. The reproducibility of these prepared dendritic columns was investigated, yielding satisfactory results.</div></div><div><h3>Significance</h3><div>For the first time, a dendritic mixed-mode stationary phase was fabricated via thiol-epoxy click reaction for detecting bisphenols in actual samples. This strategy overcomes the time-consuming and inefficient synthesis of traditional dendritic phases, offering a novel route for dendritic materials and extending their environmental applications.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1394 ","pages":"Article 345216"},"PeriodicalIF":6.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drone-based needle trap device array coupled with portable mass spectrometry for onsite analysis of hazardous air pollutants","authors":"Jianfeng Zhang ,&nbsp;Yi Li ,&nbsp;Borui Ji ,&nbsp;Haoran Liu ,&nbsp;Lei Li ,&nbsp;Wei Gao ,&nbsp;Zhen Zhou ,&nbsp;Bin Hu","doi":"10.1016/j.aca.2026.345218","DOIUrl":"10.1016/j.aca.2026.345218","url":null,"abstract":"<div><h3>Background</h3><div>Onsite detection of hazardous air pollutants is crucial for environmental monitoring, as such pollutants can be rapidly released and diffused from emission sources into the surrounding and upper air, often occurring in locations that are difficult or unsafe for human access. Traditional field sampling approaches pose challenges in providing timely molecular identification and spatial characterization of pollutants under such dynamic and hard-to-reach conditions. Therefore, the development of rapid, sensitive, and field-deployable analytical strategies for air pollutant monitoring is highly needed.</div></div><div><h3>Results</h3><div>We developed a drone-based needle trap device (NTD) array for air sampling at different environmental conditions. The NTD array enabled simultaneous sampling at multiple heights during a single flight, and samples collected by the NTD were subsequently analyzed using portable gas chromatography-mass spectrometry (GC-MS), allowing rapid identification and quantification of air pollutants within minutes. Acceptable analytical performance was achieved, including low detection limits (at ppbv level), excellent linearity (R² &gt; 0.99), good reproducibility (RSD &lt;20%, n = 6), and fast analysis speed (within minutes). Onsite environmental analysis was successfully demonstrated for air samples from biogenic, anthropogenic, and special environments involving hazardous volatile releases. In particular, the vertical diffusion behavior of VOCs was also investigated, showing the spatial distribution of air pollutants in the air.</div></div><div><h3>Significance</h3><div>The integration of a drone-based NTD array with portable GC-MS enables onsite detection and simultaneous multi-height sampling during a single flight, allowing high-resolution mapping of vertical concentration gradients of hazardous air pollutants. This approach provides an effective and cost-efficient method for characterizing pollutant distributions in complex or hard-to-access environments and offers new possibilities for field atmospheric monitoring.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1394 ","pages":"Article 345218"},"PeriodicalIF":6.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a method for determinion of organophosphorus flame retardant di-ester and hydroxylated metabolites in human urine","authors":"Xiaohong Yu ,&nbsp;Tian Qiu ,&nbsp;Jingyu Wang ,&nbsp;Qingqing Luo ,&nbsp;Tong Shen ,&nbsp;Yifu Lu ,&nbsp;Xiaoming Shi","doi":"10.1016/j.aca.2026.345224","DOIUrl":"10.1016/j.aca.2026.345224","url":null,"abstract":"<div><h3>Background</h3><div>As biomarkers for assessing human exposure to organophosphate flame retardants (OPFRs), the accurate measurement of urinary metabolites of OPFRs (mOPFRs) is critical for evaluating their health impacts.</div></div><div><h3>Results</h3><div>We established a high-throughput liquid chromatography–tandem mass spectrometry (LC-MS/MS) technique for the concurrent quantification of 14 mOPFRs in human urine, including diesters and hydroxylated metabolites. Urine samples were processed via solid-phase extraction, eluted with 2% ammoniated methanol solution, concentrated by nitrogen stream, and finally reconstituted in the original mobile phase before analysis using LC-MS/MS, executed in electrospray ionization and multiple reaction monitoring modes. High-sensitivity mass spectrometry signal acquisition was achieved based on ion fragmentation patterns. Baseline separation of isomeric compounds was accomplished by adjusting the mobile phase elution strength. Enhanced extraction efficiency and purification were based on quasi-molecular properties. The methodology exhibited method detection limits ranging from 0.018 to 0.16 μg/L. Spiked recovery rates varied between 72.2% and 109.6%, while relative standard deviations fluctuated from 5.1% to 14.7%, and matrix effects were noted to be within the range of 77.2%–112.3%. The reliability of method was validated through the analysis of certified reference materials and participation in proficiency evaluations conducted in various laboratories. This approach has been effectively utilized for analyzing urinary mOPFRs within framework of Chinese National Human Biomonitoring Program by conducting environmentally sustainable and applicable green assessment.</div></div><div><h3>Significance</h3><div>This method thoroughly optimized essential elements concerning mass spectrometry settings, chromatographic parameters, and sample preparation procedures, providing reliable technical support for assessing population exposure to OPFRs and conducting risk evaluations.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1394 ","pages":"Article 345224"},"PeriodicalIF":6.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146153234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric aptasensor based on GO-SELEX screened aptamers for detecting norfloxacin antibiotic residues in fish and shrimp","authors":"Xue Wang ,&nbsp;Zhaoyang Zhou ,&nbsp;Shuang Jiang ,&nbsp;Qi Sun ,&nbsp;Xianlu Lei ,&nbsp;Yong Xie ,&nbsp;Tao Le","doi":"10.1016/j.aca.2026.345208","DOIUrl":"10.1016/j.aca.2026.345208","url":null,"abstract":"<div><div>Norfloxacin (NOR) has been widely applied in animal husbandry and aquaculture because of its potent antibacterial activity, broad-spectrum efficacy and cost-efficient. Nevertheless, its inappropriate use may result in teratogenic and carcinogenic risks to humans, making the accurate and rapid detection of NOR residues in food critically important. In this study, a strong affinity (Kd = 84 nM) and high selectivity anti-NOR aptamer was obtained by screening with Graphene Oxide Systematic Evolution of Ligands by Exponential Enrichment (GO-SELEX), and a colorimetric aptasensor was fabricated. The limit of detection of the aptasensor was 1.04 ng/mL, and the linear range was 1.56-100 ng/mL. Average recoveries of the aptasensor in shrimp and fish sample analyses ranged from 89.33% to 103.24%, accompanied by coefficients of variation from 3.63% to 9.43%, indicating good analytical precision and accuracy. The aptasensor constructed in this study showed high consistency with the High-Performance Liquid Chromatography (HPLC) for NOR detection, indicating that it is an efficient and promising sustainable strategy for monitoring NOR.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1394 ","pages":"Article 345208"},"PeriodicalIF":6.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}