Analytica Chimica Acta最新文献

{"title":"Cascade catalysis-based signal amplification for colorimetric detection of acetylcholinesterase and its inhibitors","authors":"Runze Zhang, Kang Wang, Jinheng Wei, Lu Cheng, Zaiwei Wen, Yong Zhao, Lijun Xu, Renjun Pei","doi":"10.1016/j.aca.2025.344784","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344784","url":null,"abstract":"<h3>Background</h3>Cascade catalysis represents a fundamental physiological process and serves as a highly effective strategy for signal amplification in biosensing. Acetylcholinesterase (AChE) is a pivotal enzyme in neurological function, acting not only as a critical biomarker for neurodegenerative diseases but also as a primary target for pharmaceuticals and pesticides. Consequently, the detection of AChE activity and the screening of its inhibitors are essential for clinical diagnostics, drug development, and environmental monitoring. Reliable methods for trace-level AChE analysis remain a critical challenge that needs to be addressed promptly<h3>Results</h3>We reported a novel AChE-urease cascade catalysis amplification strategy for the colorimetric detection of AChE activity and its inhibitors. In this system, AChE catalyzes the hydrolysis of thioacetylcholine chloride (ATCh) to produce thiocholine (TCh). TCh then binds to Ag⁺ via its thiol group, thereby alleviating the inhibitory effect of Ag⁺ on urease activity. Urease with high activity catalyzes urea hydrolysis, leading to a rise in pH, which is monitored using the pH indicator phenol red. Leveraging cascade catalysis, this method achieves highly sensitive detection of AChE with a limit of detection (LOD) as low as 0.0116 mU/mL. The feasibility for inhibitor screening was validated using dipterex and berberine as model inhibitors, yielding IC₅₀ values of 28 ng/mL and 14.4 μM, and LODs of 0.394 ng/mL and 0.23 μM, respectively.<h3>Significance</h3>These results present a novel signal amplification strategy relying on enzyme cascade catalysis. This approach boasts advantages like simplicity, high sensitivity, and low cost. It not only holds promising applications in detecting AChE and its inhibitors for agriculture, medicine, and biosensing but also may extend to other enzymes that mediate thiol transformations.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"13 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Porous SERS Microneedle for Multiplex Detection of Glucose, Uric Acid, and Cholesterol in Interstitial Fluid","authors":"Geng Zhu, Yang Wang, Xi Yang, Cheng Chen, Xiaoyi Lv, Yan Huang, Xiangwei Zhao","doi":"10.1016/j.aca.2025.344788","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344788","url":null,"abstract":"To detect glucose, uric acid and cholesterol in interstitial fluid simultaneously for prognosis of metabolic syndrome (MetS), a porous surface-enhanced Raman scattering (SERS) microneedle was fabricated by integrating SERS probes and three kinds of enzyme in three independent areas of one porous microneedle, respectively. Paraffin wax was used as a hydrophobic barrier to segment a porous microneedle to three independent areas, in which gold nanoshells (used as SERS substrates), 4-mercaptophenylboronic acid (4-MPBA), and one of glucose oxidase, urate oxidase, and cholesterol oxidase were integrated, respectively, and thus SERS probes altered SERS signals under the action of enzymes on their substrates. In vitro experiments demonstrated stable and accurate detection within the ranges of 0-15 mM glucose, 0-0.9 mM uric acid, and 0-9 mM cholesterol. Animal studies confirmed its feasibility for in vivo use. This porous SERS microneedle offers a minimally invasive and convenient solution for multiplex detection and point-of-care diagnosis in patients.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"41 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145288916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy metal marker-amplified recognition analysis: enhanced water body differentiation via carbon nanomaterial fluorescence and machine learning","authors":"Jiadeng Chen, Wen Zeng, Guijiao Wen, Tao Lin, Chenghui Li, Xiandeng Hou","doi":"10.1016/j.aca.2025.344786","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344786","url":null,"abstract":"<h3>Background</h3>Traditional water monitoring methods usually track multiple elements and rely on multi-dimensional data for dimensionality reduction, but they primarily focus on assessing water resource information and spatiotemporal changes rather than effectively distinguished between regional water bodies. Distinguishing regional water bodies is essential for sustainable resource management. Therefore, it is important to establish new strategy for fast and efficient recognition analysis of water bodies.<h3>Result</h3>This study develops a novel fluorescent sensor array based on carbon nanomaterials (CDs, CNSs, CNPs) and machine learning for efficient differentiation of regional water bodies, to which heavy metal markers was added. The concept of heavy metal marker was proposed to amplify the recognition analysis, i.e., a heavy metal marker system comprising of Pb²⁺, Zn²⁺, Cd²⁺ and Ni²⁺ was engineered as signal amplifiers, markedly amplifying fluorescence disparities through competitive binding interaction between the nanomaterials and sample matrices. By combining linear discriminant analysis (LDA), the system achieved 100% classification accuracy validated by near-zero Wilks' Lambda values for both adjacent water bodies and cross-regional counterparts. The sensor exhibits exceptional anti-interference capability, accurately differentiating high-salinity and polluted water sources. Simultaneously, it can achieve high spatial resolution in water source discrimination, resolving adjacent sources separated by &lt;2 km.<h3>Significance</h3>As a proof of concept of heavy metal marker with a combination of Pb²⁺, Zn²⁺, Cd²⁺, and Ni²⁺ as an example, it was proved that adding the markers is an efficient way to achieve the best discrimination goal. This approach may provide a reliable tool for pollution source tracing, demonstrating significant potential for environmental monitoring applications in the future.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"43 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Design of CXCR4 Cyclic Peptide via Molecular Docking for 99mTc-Labeled SPECT/CT Imaging in Melanoma","authors":"Xianrui Yin, Xin Wang, Chengwei Chen, Shenglan Jia, Wanhong Li, Yihong Wu, Xin Chen, Danling Feng, Wai Ping Cecilia Tsang, Zhihao Han, Yueqing Gu","doi":"10.1016/j.aca.2025.344754","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344754","url":null,"abstract":"<h3>Backgroud</h3>The chemokine receptor CXCR4 is a crucial target for the diagnosis and treatment of cancer due to its close association with tumor growth, metastasis, invasiveness, and prognosis.<h3>Results</h3>The cyclic peptides KC1 and KC1E, along with their radiolabeled conjugates [^99mTc]Tc-HYNIC-KC1 and [^99mTc]Tc-HYNIC-KC1E, were successfully synthesized. The binding affinity of KC1 toward the CXCR4 receptor was evaluated using Surface Plasmon Resonance (SPR). Furthermore, key physicochemical and biological properties of the radiotracers were systematically assessed, including the oil-water partition coefficient (LogP), in vitro stability in normal saline solution and mouse serum, in vivo SPECT/CT imaging performance in a B16-F10 tumor-bearing mouse model, and biodistribution profiles following intravenous administration.<h3>Significance</h3>A novel cyclic peptide, KC1, was designed and shown to exhibit selective binding to the CXCR4 receptor. Molecular docking simulations were performed to elucidate the potential interaction mechanism between KC1 and CXCR4. The radiolabeled derivative [^99mTc]Tc-HYNIC-KC1E was synthesized and demonstrated favorable in vivo imaging performance in B16-F10 tumor-bearing mice, supporting its potential as a promising imaging agent for CXCR4-positive tumors.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"29 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Mercury in Single Mammalian Cells at Attogram Level by SC-ICP-MS","authors":"Xueyan Wang, Po Tang, Hao Liu, Yingying Li, Hua Guo, Pengyang Li, Deming Han, Nian Liu, Lihong Liu, Bin He, Ligang Hu, Guibin Jiang","doi":"10.1016/j.aca.2025.344787","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344787","url":null,"abstract":"<h3>Background</h3>Mercury (Hg), a global pollutant, poses health risks to humans and mammals even at low exposure levels. However, current analytical methods face challenges in quantifying cellular Hg at ultralow concentrations. In this study, we developed a sensitive single-cell inductively coupled plasma-mass spectrometry (SC-ICP-MS) method by utilize a temperature-controlled introduction system to trace Hg in individual mammalian cells.<h3>Results</h3>Sensitivity was significantly enhanced through a personalized tuning process, which increased the instrument sensitivity of Hg ions (Hg²⁺) by 28.8%. Through optimization of detection conditions, we achieved an improved transport efficiency (TE) of 27.3% for single-cell detection in THP-1 cells. By implementing the comprehensively optimized method, we attained an exceptionally low single-cell-level Hg mass detection limit (<em>LOD</em>_<em>m</em>) of 0.01 fg per cell, coupled with a cell density detection limit (<em>LOD</em>_<em>d</em>) of 8.1×10² cells mL^-1, resulting in a Hg concentration detection limit (<em>LOD</em>_<em>c</em>) of 0.008 ng L^-1. This validated method demonstrated robust applicability across multiple mammalian cell types, revealing that Hg content (<em>m</em>) at the single-cell level exhibited exponential growth with increasing exposure concentration, while the heterogeneity of Hg displayed an initial rise before reaching a plateau or decreasing.<h3>Significance</h3>This study establishes a highly sensitive and reproducible method for monitoring single-cell Hg content and heterogeneity at environmentally low exposure levels. The technical advances provide a robust methodological foundation for assessing element-specific toxicity across different mammalian cell types, supporting the health risk evaluation in low-dose scenarios.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"9 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145288915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flexible MoS2-Based Wearable SERS Sensor for Noninvasive Early Diagnosis of Glutaric Acidemia","authors":"Qi Liu, Wenrou Yu, Luo Ren, Anping Liu, Zhaoxian Chen, Xuliang Xia, Yuqin Xie, Yingzhou Huang","doi":"10.1016/j.aca.2025.344776","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344776","url":null,"abstract":"&lt;h2&gt;Section snippets&lt;/h2&gt;&lt;section&gt;&lt;section&gt;&lt;section&gt;&lt;h2&gt;Materials&lt;/h2&gt;High purity molybdenum foil (0.03 mm × 100 mm × 100 mm Mo (≥99.999%)), glutaric acid (analytical standard, 99.2%), cetyltrimethylammonium bromide (CTAB, 99% biotech grade), methyl blue (MB), rhodamine 6G ( ≥ 99%) were purchased from Shanghai McLean Biochemical Technology Co., Ltd&lt;strong&gt;.&lt;/strong&gt; Thiourea ( ≥ 99%), acetone (AR, analytically pure), artificial urine, H&lt;sub&gt;2&lt;/sub&gt;SO&lt;sub&gt;4&lt;/sub&gt; ,and absolute ethanol ( ≥ 99.5%) were purchased from Aladdin (Shanghai) Biochemical Technology Inc. Distilled water (Millipore Milli-Q grade)&lt;/section&gt;&lt;/section&gt;&lt;/section&gt;&lt;section&gt;&lt;section&gt;&lt;section&gt;&lt;h2&gt;Optimized Synthesis of MoS2 Nanoflowers via H&lt;sup&gt;+&lt;/sup&gt; dosage Tuning&lt;/h2&gt;Figure 1 displays SEM images of MoS&lt;sub&gt;2&lt;/sub&gt; substrate. As shown in Fig. 1a, without the addition of H&lt;sub&gt;2&lt;/sub&gt;SO&lt;sub&gt;4&lt;/sub&gt;. MoS&lt;sub&gt;2&lt;/sub&gt; initially grows as nanosheets on the molybdenum foil surface. Upon adjusting the H&lt;sup&gt;+&lt;/sup&gt; concentration, specifically by adding 0.1 mL of H&lt;sub&gt;2&lt;/sub&gt;SO&lt;sub&gt;4&lt;/sub&gt; (Fig. 1b), uniform MoS&lt;sub&gt;2&lt;/sub&gt; nanoflowers form on the nanosheets. By varying the amount of H&lt;sub&gt;2&lt;/sub&gt;SO&lt;sub&gt;4&lt;/sub&gt; added, we obtained MoS&lt;sub&gt;2&lt;/sub&gt; substrate with distinct morphologies (Supplementary Information Fig. S1a). The experimental results demonstrate that the amount of H&lt;sub&gt;2&lt;/sub&gt;SO&lt;sub&gt;4&lt;/sub&gt;&lt;/section&gt;&lt;/section&gt;&lt;/section&gt;&lt;section&gt;&lt;section&gt;&lt;h2&gt;Conclusion&lt;/h2&gt;In summary, we successfully synthesized a highly sensitive, flexible MoS&lt;sub&gt;2&lt;/sub&gt; substrate using a simple hydrothermal method. This flexible substrate can be integrated into baby diapers to collect infant urine, enabling non-invasive detection via SERS technology. The flexible MoS&lt;sub&gt;2&lt;/sub&gt; substrate demonstrates a detection limit (LOD) for biomarkers as low as 3.29 nM and EF up to 8.36×10&lt;sup&gt;6&lt;/sup&gt;, with high uniformity and experimental reproducibility. We employed this substrate to detect glutaric acid in artificial&lt;/section&gt;&lt;/section&gt;&lt;section&gt;&lt;section&gt;&lt;h2&gt;CRediT authorship contribution statement&lt;/h2&gt;&lt;strong&gt;Yingzhou Huang:&lt;/strong&gt; Conceptualization, Formal analysis, Funding acquisition, Supervision, Writing – review &amp; editing. &lt;strong&gt;Zhaoxian Chen:&lt;/strong&gt; Data curation, Formal analysis. &lt;strong&gt;Xuliang Xia:&lt;/strong&gt; Conceptualization, Data curation, Formal analysis, Project administration, Writing – review &amp; editing. &lt;strong&gt;Qi Liu:&lt;/strong&gt; Data curation, Formal analysis, Investigation, Writing – original draft. &lt;strong&gt;Yuqin Xie:&lt;/strong&gt; Data curation, Formal analysis. &lt;strong&gt;Wenrou Yu:&lt;/strong&gt; Data curation, Formal analysis, Investigation. &lt;strong&gt;Luo Ren:&lt;/strong&gt; Conceptualization, Formal&lt;/section&gt;&lt;/section&gt;&lt;section&gt;&lt;section&gt;&lt;h2&gt;Declaration of competing interest&lt;/h2&gt;The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.&lt;/section&gt;&lt;/section&gt;&lt;section&gt;&lt;section&gt;&lt;h2&gt;Data availability&lt;/h2","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"73 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of Suspect and Non-target Screening of Agriculture-Related Organic Contaminants","authors":"Irantzu Vergara-Luis, Laura Righetti, Maitane Olivares, Federico Padilla-Gonzalez, Ailette Prieto, Ane Arrizabalaga-Larrañaga, Juan F. Ayala-Cabrera","doi":"10.1016/j.aca.2025.344774","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344774","url":null,"abstract":"<h3>Background</h3>Production systems that aim to reduce waste and promote the reuse of materials contribute to more sustainable food systems. However, these approaches can also lead to the accumulation and recirculation of chemical hazards from organic waste to the soil and, ultimately, to food. To address these risks and assess the safety and effectiveness of waste reuse in agriculture, non-target screening (NTS) and suspect screening (SS) analytical methodologies are essential, as they enable the detection of chemical residues, pollutants, and unintended by-products in soil, crops, and compost.<h3>Results</h3>A literature review covering the period 2021–2025 was conducted to identify recent analytical advances and the application of SS and NTS strategies in food production systems. The review highlights key analytical challenges associated with traditional targeted analysis and explores the improvements achieved by SS and NTS approaches. Emphasis is placed on advancements in sample treatment (extraction and clean-up), instrumentation , and data processing and analysis tools. Furthermore, the review provides an overview of chemical hazards identified using SS and NTS methodologies within the context of agri-food systems.<h3>Significance</h3>The review highlights significant progress in the development of SS and NTS strategies for the identification of known and unknown hazardous chemicals. However, it also reveals a limited number of studies applying these approaches specifically within sustainable agriculture frameworks. Despite this gap, the potential of SS and NTS to support safer and more sustainable practices in agri-food systems suggests that their broader application could be of considerable interest to the scientific community in future research.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"4 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TICT-activated viscosity sensitive mitochondrial probes for imaging and early diagnosis of drug-induced liver injury","authors":"Jie Wang, Lifeiyang Wan, Kaiyuan Liu, Qi Su, Xinyue Zhao, Cheng Huang, Yunjia Zhao, Lei Hu, Hui Wang","doi":"10.1016/j.aca.2025.344778","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344778","url":null,"abstract":"<h3>Background</h3>Drug-induced liver injury (DILI) poses a significant clinical challenge, with mitochondrial dysfunction and elevated microviscosity emerging as key pathological features.<h3>Results</h3>We reported the rational design and synthesis of a series of viscosity-responsive fluorescent probes <strong>WACn</strong> (n = 2, 4, 6, 8, 10), based on a D-π-A architecture utilizing the twisted intramolecular charge transfer (TICT) mechanism. These probes integrated a carbazole-thiophene electron-donating unit and a benzoindolium acceptor modified with varying alkyl chains to tune intramolecular rotation and viscosity responsiveness. Study results revealed that probes <strong>WACn</strong> (n = 2, 4, 6, 8, 10) exhibited strong viscosity-dependent fluorescence enhancement, robust photostability, low cytotoxicity, and excellent mitochondrial targeting specificity. Notably, probe <strong>WAC2</strong> enabled mitochondrial imaging at an ultralow concentration (30 nM) and functioned independently of membrane potential. Besides, <strong>WAC2</strong> successfully reported viscosity changes under multiple drug stimulations (PA, H₂O₂, Cu²⁺, dexamethasone, erastin, etc.) as well as antifungal drug (nystatin-induced) and inflammation (lipopolysaccharide-induced), both in cells or in vivo. Meanwhile, <strong>WAC2</strong> enabled monitor changes in viscosity during APAP-induced drug-induced injury in normal hepatocytes as well as hepatoma cells. Most importantly, it enabled the multi-level visualization of DILI progression in BALB/c mice, including tissue, organ and living levels.<h3>Significance</h3>Collectively, probe <strong>WAC2</strong> provides a powerful platform for real-time, in situ monitoring of mitochondrial viscosity and offers valuable insights for early diagnosis and mechanistic investigation of DILI.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"21 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent Visualization of Endoplasmic Reticulum Stress Induced by Microplastic Pollution via Surge of Intracellular Reactive Oxygen Species","authors":"Le Yang, Xinchao Xia, Fan Li, Qiuhong Mo, Pin Shang, Junbin Li, Yi Li, Yibo Zhou, Zhihe Qing","doi":"10.1016/j.aca.2025.344777","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344777","url":null,"abstract":"<h3>Background</h3>Excessive accumulation of microplastics (MPs) inside the body can induce serious diseases, and the potential pathological mechanism was related to endoplasmic reticulum (ER) stress induced by the surge of intracellular reactive oxygen species (ROS). However, until now, the invasion of MPs triggering this unique process remains elusive.<h3>Results</h3>Herein, peroxynitrite (ONOO^-), was selected as the model target, and an ER-targeted fluorogenic probe (ER-NA-PB) was constructed for real-time visualizing the fluctuation of ONOO^- during cellular ER stimulated by MPs. ER-NA-PB exhibited excellent sensitivity (15.2 nM) and selectivity toward ONOO^- in aqueous solution, and the exogenous and endogenous ONOO^- fluctuation in ER also can be visualized by this potently fluorescent tracing tool. In addition, ER-NA-PB revealed that the content of ONOO^- in ER during stimulation of MPs was elevated with increase of MPs concentration and prolongation of stimulation time.<h3>Significance</h3>For the first time, the fluctuation of active molecule in embryonic tissue was revealed by fluorescence probe, and this research provides a promising tool for deeper studying the pathological mechanisms underlying relevant diseases caused by MPs pollution inducing ER stress.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"119 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic monitoring of Cytochrome c in single tumor cell for anticancer drug susceptibility assessment","authors":"Lifen Long, Huiqian Zhou, Hao Tan, Hongtian Zeng, Qingqing Zhang, Tingting Hao, Zhiyong Guo","doi":"10.1016/j.aca.2025.344763","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344763","url":null,"abstract":"<h3>Background</h3>The release of Cytochrome <em>c</em> (Cyt <em>c</em>) from mitochondria into the cytosol is a key event in the intrinsic apoptosis pathway induced by chemotherapeutic agents. The dynamic monitoring of intracellular Cyt <em>c</em> levels is therefore of significant clinical importance for understanding tumor cell apoptosis.<h3>Results</h3>Herein, we developed a highly sensitive and selective nanoelectrochemical biosensor for the in situ monitoring of Cyt <em>c</em> dynamics in a single living cell. The sensor was constructed by modifying a Platinum nanoelectrode (PtNE) with a Cyt <em>c</em>-specific aptamer (Apt), denoted as PtNE/Apt. Employing Fast-scan voltammetry (FSV) at an ultra-high scan rate of 10 kV/s, the biosensor achieved a remarkable detection limit of ∼1.0 × 10^-13 mol/L for Cyt <em>c</em>. Using this platform, we performed real-time single-cell analysis, revealing that the intracellular concentration of Cyt <em>c</em> increased from undetectable levels to 1.8 μmol/L, while cell viability decreased from 99.9% to 45.1%, within 60 min of Cisplatin (CDDP) treatment. Furthermore, the platform enabled the evaluation of chemotherapeutic efficacy, ranking the susceptibility of HeLa cells to four drugs as follows: CDDP &gt; Paclitaxel (PTX) &gt; Doxorubicin (DOX) &gt;Resveratrol (RSV).<h3>Significance and Novelty</h3>This aptamer-based nanoelectrochemical sensing strategy provides a powerful and high-precision tool for the dynamic analysis of apoptosis-related molecules, offering profound insights into the mechanisms of chemotherapeutic action and holding great potential for applications in individualized treatment screening.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"30 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145255133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}