Analytica Chimica Acta最新文献

{"title":"Tiny but Mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a Push-Pull Fluorescent Dye for Lipid Droplet Imaging","authors":"Wen Ann Wee, Tatum Melati Andini, Tomotaka Kumagai, Shanmugavel Chinnathambi, Ganesh N. Pandian, Arthur Millius, Hiroshi Sugiyama, Daron M. Standley, Soyoung Park","doi":"10.1016/j.aca.2025.344130","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344130","url":null,"abstract":"Lipid droplets (LDs) are ubiquitous cellular organelles with a neutral lipid core containing triacylglycerols and cholesteryl esters surrounded by phospholipids. Recent findings indicate that LDs are intricately linked to diseases, such as cancer and neurological disorders, in addition to their roles in cellular senescence and immune responses. Herein, we describe a simple yet robust push-pull molecule, <em>N,N</em>-dimethyl-4-(5-nitrothiophen-2-yl)aniline (<strong>NiTA</strong>), as a versatile LD fluorescent probe. <strong>NiTA</strong> showed an absorption spectrum with a substantial bathochromic shift and a fluorescence spectrum with excellent solvatochromism. Leveraging the remarkable photophysical features of <strong>NiTA</strong>, we stained LDs in major immune cells, including T and B cells, and macrophages, and monitored the changes in LDs under oxidative and starvation conditions. Furthermore, we demonstrated the applicability of <strong>NiTA</strong> for visualizing the organization of medaka fish (<em>Oryzias latipes</em>) embryos during development. We expect the small yet powerful <strong>NiTA</strong> to be utilized in various applications, including fluorescence mapping to observe LD numbers, morphology, and polarity changes in animals and cells.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"23 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143885342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A modified spectral standardization method for uranium ores measurement using laser induced breakdown spectroscopy","authors":"Jianxun Ji ,&nbsp;Zongyu Hou ,&nbsp;Weilun Gu ,&nbsp;Xiang Yu ,&nbsp;Zhe Wang","doi":"10.1016/j.aca.2025.344125","DOIUrl":"10.1016/j.aca.2025.344125","url":null,"abstract":"<div><h3>Background</h3><div>The high signal uncertainty is the key factor hindering the quantification performance of laser induced breakdown spectroscopy (LIBS) technology. Different plasma parameter compensation algorithms for reducing signal uncertainty have been proposed. Among them, spectral standardization is a representative, which can reduce signal uncertainty by compensating for the variations of plasma parameters, especially using multiple independent spectral lines of the measured element to compensate for the fluctuation of total number density. However, it's hard to find enough independent lines for cases such as uranium in ores measurement. This work proposes an updated version to reduce the signal uncertainty for these cases.</div></div><div><h3>Results</h3><div>On the basis of spectral standardization method, the new method uses another element information of the matrix to compensate the fluctuation of total number density for the measured element. Specifically, multiple spectral lines of another element are first used to characterize its total number density, and then the total number density of the measured element is obtained through the correlation between the two elements. Finally, the new method is evaluated on uranium ores. Results showed that after using the information of the silicon element to estimate the total number density of uranium, the relative standard deviation(RSD) of the U II 409.01 nm was reduced from the original 23.03 %% to 9.41 %, the quantitative model determination coefficient (<span><math><mrow><msubsup><mi>R</mi><mi>C</mi><mn>2</mn></msubsup></mrow></math></span>) of uranium was increased from the original 0.9129 to 0.9921, and the root mean square error of prediction (<span><math><mrow><mi>R</mi><mi>M</mi><mi>S</mi><msub><mi>E</mi><mi>P</mi></msub></mrow></math></span>) was reduced from the original 0.2586 to 0.0670.</div></div><div><h3>Significance</h3><div>The modified spectral standardization method, which using another element information to estimate the total number density of the measured element, can greatly reduce the signal uncertainty and improve the quantitative analysis performance of uranium in ores. What's more, it provides a new signal uncertainty reduction method for elements without enough independent spectral lines, such as the spectral with severe interference, which expands the application scope of spectral standardization method.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1359 ","pages":"Article 344125"},"PeriodicalIF":5.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143880796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activatable optical probe for non-invasive detection of APN activity in diabetes and its complications","authors":"Weibo Zhao ,&nbsp;Lidan Lu ,&nbsp;Nannan Song ,&nbsp;Kai Wang ,&nbsp;Yuqing Sun ,&nbsp;Peijuan Wang","doi":"10.1016/j.aca.2025.344122","DOIUrl":"10.1016/j.aca.2025.344122","url":null,"abstract":"<div><h3>Background</h3><div>Diabetes is a major global health issue, primarily characterized by chronic hyperglycemia, and can lead to severe complications such as ovarian dysfunction. Early and accurate diagnosis is critical for effective disease management and prevention of long-term complications. This study explores the clinical potential of <strong>Hcy-APN</strong>, a diagnostic probe designed to detect aminopeptidase N (APN) activity in solution, cells, and in vivo.</div></div><div><h3>Results</h3><div>The Hcy-APN probe incorporates a hemicyanine moiety, remaining non-fluorescent until cleaved by APN, which enhances both fluorescence and photoacoustic (PA) signals. In vitro experiments demonstrated a strong correlation between APN concentration and PA intensity, with minimal interference from other bioenzymes. The probe exhibited high stability and consistent PA signals, enabling deep tissue imaging at depths up to <strong>0.6 cm</strong>. Cellular imaging confirmed its low cytotoxicity, while in vivo studies in diabetic mice highlighted its effectiveness in monitoring APN activity. Additionally, fluorescence assays of human clinical samples revealed significantly higher signals in serum from patients with ovarian dysfunction, suggesting the probe's diagnostic utility in diabetes-related ovarian conditions.</div></div><div><h3>Significance</h3><div>This study underscores the potential of <strong>Hcy-APN</strong> as a valuable tool for clinical diagnostics, particularly in monitoring diabetes-associated complications like ovarian dysfunction. The probe's dual-modality imaging capability (fluorescence and PA) enhances its applicability in deep tissue analysis. Further research is needed to optimize its clinical use and validate its efficacy in improving patient outcomes.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1359 ","pages":"Article 344122"},"PeriodicalIF":5.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143885345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescence Detection of Phenylethylamine Based on Cucurbit[7]uril Supramolecular Assemblies","authors":"Yan Sun, Shang-Wei Yuan, Qing Chen, Dan-Ping Chen, Xue-Yang Xu, Zhu Tao, Xin Xiao","doi":"10.1016/j.aca.2025.344126","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344126","url":null,"abstract":"<h3>Background</h3>Phenethylamine (PEA) is a novel psychoactive substance that can cause agitation and hallucinations, posing a threat to human health. In this study, a N1@Q[7] supramolecular assembly with a robust fluorescent probe effect was prepared through the supramolecular self-assembly of cucurbit[7]uril (Q[7]) and a bromophenylpyridine derivative (N1).<h3>Results</h3>In this supramolecular assembly, the isoquinoline moiety of N1 entered the cavity of Q[7], while the 4,4-bromophenylpyridyl moiety remained outside, forming a 1:1 inclusion complex. It was found that the addition of other amines did not significantly affect the fluorescence of the assembly, but the addition of PEA caused a notable decrease in fluorescence intensity and a red shift. N1@Q[7] demonstrated sensitive specificity and high selectivity to PEA. The principle is that PEA molecules, N1 molecules, and Q[7] form a synergistic ternary system. Based on this, the detection of PEA in real urine samples by N1@Q[7] was simulated.<h3>Signiffcance and Novelty</h3>The probe exhibited high selectivity in the presence of major components of real urine and enabled visual detection of PEA through fluorescence color change at different concentrations. This study provides a new fluorescent probe method for the detection of drugs and their metabolites.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"10 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143885344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaporation dynamics of sessile droplets in the ablation cell for LA-ICP-MS analysis of liquids","authors":"Petr Rudolf ,&nbsp;Kristýna Lapčíková ,&nbsp;Alena Randová ,&nbsp;Antonín Kaňa","doi":"10.1016/j.aca.2025.344127","DOIUrl":"10.1016/j.aca.2025.344127","url":null,"abstract":"<div><h3>Background</h3><div>Inductively coupled plasma mass spectrometry (ICP-MS) is a powerful method for elemental analysis. However, it faces challenges when analysing organic-rich samples due to plasma instability. Laser ablation coupled with ICP-MS (LA-ICP-MS) offers a suitable alternative by introducing minimal sample amounts into the plasma, maintaining its stability. In the case of liquids, rapid evaporation of liquid droplets limits the applicability of LA-ICP-MS, as it impacts analyte concentration. Understanding droplet evaporation dynamics within the ablation cell, where helium accelerates droplet evaporation, is essential to improve reliability of the analysis. This study explores the evaporation behaviour of organic solvent droplets in LA-ICP-MS. In addition, it aims to enhance liquid sample analysis by modelling observed evaporation processes.</div></div><div><h3>Results</h3><div>Experiments were conducted with organic solvent droplets evaporating on various sample-holder surfaces, focusing on evaporation within helium and air environments. Polytetrafluoroethylene (PTFE) was identified as the optimal surface material for sustaining high contact angles of sessile droplets, thus prolonging droplet lifetime. A mathematical model was developed to describe evaporation, incorporating contact angle, droplet geometry, and mass transfer dynamics. The model demonstrates that most droplets initially evaporate in a constant contact radius mode, then transition to a constant contact angle mode. The experimental data align closely with the model predictions, affirming the impact of initial contact angle and droplet volume on evaporation behaviour. Additionally, we established correlations to predict droplet lifetime based on saturated vapour pressure and initial droplet height, contributing a practical reference for management of organic solvents in LA-ICP-MS applications.</div></div><div><h3>Significance and novelty</h3><div>This research provides a straightforward approach to understanding and controlling evaporation dynamics of liquid samples in LA-ICP-MS, which is essential for reliable elemental analysis of organic-rich liquid samples. By modelling sessile droplet behaviour and validating it with experimental data, this study lays the groundwork for a wide range of applications, such as the analysis of metals in oils and other low-volatility organic solvents or analysis of microliter-scale liquid samples.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1359 ","pages":"Article 344127"},"PeriodicalIF":5.7,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-simple nucleic acid extraction using a polystyrene microplastic particles-thermal lysis system for rapid detection of pathogen in nasal mucus","authors":"Yang Li ,&nbsp;Xuecheng Yang ,&nbsp;Yajing Zhang ,&nbsp;Cuiping Ma ,&nbsp;Chao Shi","doi":"10.1016/j.aca.2025.344123","DOIUrl":"10.1016/j.aca.2025.344123","url":null,"abstract":"<div><div>Respiratory infections are a leading cause of death and disability globally and have become an important issue of public concern. Nucleic acid amplification test (NAAT) has been recognized as the gold standard for respiratory infection diagnosis, and played a critical role in epidemic control during the COVID-19 pandemic. However, the laborious nucleic acid extraction limits the application of NAAT in the on-site respiratory infection diagnosis, an effective approach for disease control and prevention. Herein, a polystyrene microplastic particle (PSMP)-thermal lysis system was established to extract nucleic acids from nasal mucus in 4 min simply and rapidly without sacrificing sensing performance. The PSMP-thermal lysis system showed a strong protein adsorption capacity, by which nearly eliminating the interference of protein content in nasal mucus on amplification reaction. Moreover, the PCR using this PSMP-thermal lysis system showed excellent selectivity and anti-jamming ability, as well as the high sensitivity comparable to that using commercial kits based on commonly used solid-phase extraction in detecting target pathogens in sticky nasal mucus with protein content of 25.11 μg/μL. Furthermore, the practical use investigation indicated the PCR assay using this system could accurately identify respiratory infection patients by detecting corresponding pathogens in clinical nasal mucus samples. This PSMP-thermal lysis system has the potential for pathogen detection in various respiratory secretions and is anticipated to substantially simplify molecular diagnosis of respiratory infections.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1359 ","pages":"Article 344123"},"PeriodicalIF":5.7,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spark Mapping Analysis for Large Samples methodology for quantitative full-scale element imaging of bearing steel bloom with varying reduction processes","authors":"Xiaofen Zhang, Liang Sheng, Yong Lyu, Bao Yang, Hui Zhang, Yunhai Jia, Haizhou Wang","doi":"10.1016/j.aca.2025.344128","DOIUrl":"https://doi.org/10.1016/j.aca.2025.344128","url":null,"abstract":"<h3>Background</h3>The characterization of chemical compositions in full-scale large size metal materials with varying processes is helpful for the rapid selection of the optimal processes in the improvement and development of new materials. The process of soft and heavy reduction processes at the end of solidification has proven to be an effective method for enhancing the internal quality of bearing steel. However, traditional analytical methods fail to accurately determine the composition distribution of full-scale large-size metal samples due to limitations in spatial resolution or time-consuming.<h3>Results</h3>The Spark Mapping Analysis for Large Samples (SMALS) technique has been used for element imaging of full-scale GCr15 bearing steel bloom section with varying reduction processes. The experimental conditions, including testing energy, the distance between the sample and the electrode, were systematically optimized to improve the repeatability and accuracy of the SMALS methodology. Six tailor-made Fe-based reference materials with concentration gradient were mapped using SMALS to establish calibration curve, and the results were validated by conducting spark atomic emission spectroscopy (Spark-AES). Additionally, the detection limits for elements such as C, Si, Mn, Cr, Ni, Mo, Cu, V, Ti, Nb, and Al were determined using SMALS. The quantitative element imaging of the cross and longitudinal sections of bearing steel bloom demonstrated the presence of frame-type and V-type segregation and indicated that the short-range reduction process could better alleviate V-type segregation compared to the long-range reduction process.<h3>Significance</h3>This study provides a successful application case of production process optimization by comparing the composition distribution of sample sections with varying reduction processes. Furthermore, the SMALS technology not only identified segregation patterns but also detected surface defects in samples. This versatile elemental imaging technique provides an efficient approach for composition analysis and process evaluation of full-scale samples, contributing significantly to the research and development of new materials.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"31 1","pages":""},"PeriodicalIF":6.2,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A diarylethene-based probe with HSO3−-activated fluorescence to photochromism: Its imaging application in living cells and zebrafish","authors":"Yinjie Wu ,&nbsp;Zhihong Gong ,&nbsp;Xiaoqian Wu ,&nbsp;Yiyan Huang ,&nbsp;Leilei Sun ,&nbsp;Haichang Ding ,&nbsp;Yu-Ling Zeng ,&nbsp;Congbin Fan ,&nbsp;Gang Liu ,&nbsp;Shouzhi Pu","doi":"10.1016/j.aca.2025.344114","DOIUrl":"10.1016/j.aca.2025.344114","url":null,"abstract":"<div><div>Sulfur dioxide (SO₂) and its derivatives (sulfite SO₃²⁻ and bisulfite HSO₃⁻) have important applications in industrial production and food preservation, but excessive intake can be hazardous to human health. Therefore, the development of highly sensitive and selective HSO₃⁻ detection methods is essential to safeguard food safety and public health. Conventional HSO₃⁻ probes mainly rely on colorimetric or fluorescence detection, but these methods have obvious limitations in that the fluorescence signal disappears as soon as the UV irradiation is stopped, and they cannot provide long-lasting and readable detection results. Herein, a fluorescent probe (<strong>DP-1)</strong> constructed from diarylethene, fluorophores and ion recognition sites were designed and developed. <strong>DP-1</strong> emits yellow fluorescence emission maximum at 600 nm in initial state, which is significantly quenched upon specific recognition of HSO₃⁻. This recognition process demonstrates <strong>DP-1</strong>'s good ion selectivity, interference resistance, and low detection limit of 16 nM. Notably, unlike conventional probes, <strong>DP-1</strong> exhibits superior fluorescence properties before recognizing HSO₃⁻, with no detectable photochromism. After ion recognition, its photochromic function is activated, while fluorescence completely disappears, demonstrating a switch from fluorescence to photochromism triggered by HSO₃⁻. The mechanism of this recognition process was confirmed by nuclear magnetic titration, high-resolution mass spectrometry, and theoretical calculations. Additionally, <strong>DP-1</strong> has been successfully applied for HSO₃⁻ detection in both live cellular and zebrafish imaging, with its excellent biocompatibility providing a reliable tool for in vivo imaging.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1359 ","pages":"Article 344114"},"PeriodicalIF":5.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vis-NIR spectra-image transformation based on circular spectral mapping for measurement of particulate matter concentration","authors":"Tiantian Pan ,&nbsp;Xiaorong Dai ,&nbsp;Wei Wang ,&nbsp;Yuan Wang ,&nbsp;Hang Xiao ,&nbsp;Fei Liu","doi":"10.1016/j.aca.2025.344113","DOIUrl":"10.1016/j.aca.2025.344113","url":null,"abstract":"<div><h3>Background</h3><div>The fine particulate matter (PM_2.5), one of the most concerned airborne pollutants, significantly impacts air quality and human health. The potential hazard of PM_2.5 related to its concentration, while the traditional methods for PM concentration measuring were expensive, time-consuming, while low-cost sensors often suffer from poor accuracy and stability. Therefore, there is a great need for a rapid, precise and stable measurement method for filter-based PM_2.5.</div></div><div><h3>Results</h3><div>We propose a novel spectra-image transformation and fusion method for filter-based PM_2.5 measurement using a portable visible near infrared (Vis-NIR) spectrometer. Traditional machine learning models based on spectra alone achieved low accuracy (R²_p &lt; 0.8). To improve performance, we introduced the circular spectral mapping (CSM) method to transform PM_2.5 spectra into CSM images, which were processed using ResNet-18, ShuffleNet V2, and MobileNet V2 networks with an attention mechanism module. The optimal model, ShuffleNetV2_Attn, improved R²_p to 0.9935. To furtherly improve the model stability, the numerical and graphical feature fusions were conducted, and the ShuffleNetV2_Attn was selected as optimal feature extractor of CSM images. The machine learning models were built based on fusion features, and the optimal model was the partial least squares (PLS) model based on fusion features extracted by successive projections algorithm (SPA), of which the R²_p, RMSEP and mean absolute percentage error (MAPE_p) were 0.9947, 6.0213 μg/m³ and 4.17 %, demonstrating high accuracy and stability overall concentration range.</div></div><div><h3>Significance</h3><div>The proposed spectra-image transformation and fusion method greatly improved the accuracy and efficiency of filter-based PM_2.5 measurement. It overcome the limitations of spectral-based machine learning methods, which often fail to capture full-band characteristics, and provides a new approach for integrating numerical and graphical spectral information.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1359 ","pages":"Article 344113"},"PeriodicalIF":5.7,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143872151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Target inhibition of rolling circle amplification generates G43 long nanowires for reverse signaling determination of alkaline phosphatase","authors":"Shuyu Zhu,&nbsp;Shiying Zhou,&nbsp;Liyuan Deng,&nbsp;Jiangbo Dong,&nbsp;Tao Gu,&nbsp;Xinyu He,&nbsp;Liang Jin,&nbsp;Changjun Hou,&nbsp;Danqun Huo","doi":"10.1016/j.aca.2025.344077","DOIUrl":"10.1016/j.aca.2025.344077","url":null,"abstract":"<div><h3>Background</h3><div>Alkaline phosphatase (ALP) can catalyze the hydrolysis of phosphate esters under alkaline conditions, mediate the dephosphorylation of substances such as proteins and nucleic acids. ALP can participate in physiological activities such as bone mineralization, cell division, and immune regulation. Abnormal ALP activity is associated with various diseases, making it an important clinical biomarker. In recent years, highly sensitive detection technologies based on isothermal amplification have developed rapidly. The Rolling Circle Amplification (RCA) technology uses T4 DNA ligase to connect the 5′-phosphate group and 3′-hydroxyl terminus of the padlock probe into a ring, achieving efficient amplification through continuous rolling circle replication.</div></div><div><h3>Results</h3><div>Leveraging this characteristic, we developed a highly sensitive sensing method (RCA-LG) based on RCA-generated G43 long nanowires for ALP detection. T4 DNA ligase circularizes the RCT into a template. Subsequently, Phi29 DNA polymerase amplifies the primer along the circular template, producing G43 long nanowires that bind with Thioflavin T (ThT) to generate fluorescent signals. When ALP is present, it dephosphorylates the 5′ end of RCT, preventing circular template formation and thereby inhibiting the amplification reaction. The RCA-LG method can detect ALP concentrations as low as 1.0 × 10⁻⁶ U/mL within 2.5 h. We propose a novel one-step RCA approach that integrates ligation and amplification reactions into a single step, simplifying operations while enhancing amplification efficiency. Furthermore, we physically separated the ALP dephosphorylation and RCA processes to avoid potential contamination from repeated sample handling.</div></div><div><h3>Significance and novelty</h3><div>This strategy takes advantage of the characteristics of RCA without the need for redundant phosphorylation modifications. Moreover, we have proposed an improved one-step RCA method combined with physical partitioning, enabling highly sensitive and specific detection of ALP and allowing the detection of this target in complex samples. Furthermore, the RCA-LG strategy can also play an important role in the screening of enzyme inhibitors.</div></div>","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":"1359 ","pages":"Article 344077"},"PeriodicalIF":5.7,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}