用于间质液中葡萄糖、尿酸和胆固醇多重检测的多孔SERS微针

IF 6 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2025-10-14 DOI:10.1016/j.aca.2025.344788

Geng Zhu, Yang Wang, Xi Yang, Cheng Chen, Xiaoyi Lv, Yan Huang, Xiangwei Zhao

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引用次数: 0

摘要

为了同时检测间质液中的葡萄糖、尿酸和胆固醇，以预测代谢综合征（MetS）的预后，将SERS探针和三种酶分别整合在一个多孔微针的三个独立区域，制成了一种多孔表面增强拉曼散射（SERS）微针。用石蜡作为疏水屏障，将多孔微针分割成三个独立的区域，其中金纳米壳（用作SERS底物）、4-巯基苯硼酸（4-MPBA）以及葡萄糖氧化酶、尿酸氧化酶和胆固醇氧化酶中的一种分别被整合，从而SERS探针在酶对其底物的作用下改变SERS信号。体外实验表明，在0- 15mm葡萄糖、0-0.9 mM尿酸和0- 9mm胆固醇范围内，检测稳定、准确。动物实验证实了其在体内使用的可行性。这种多孔SERS微针为患者的多重检测和即时诊断提供了一种微创和方便的解决方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A Porous SERS Microneedle for Multiplex Detection of Glucose, Uric Acid, and Cholesterol in Interstitial Fluid

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A Porous SERS Microneedle for Multiplex Detection of Glucose, Uric Acid, and Cholesterol in Interstitial Fluid

To detect glucose, uric acid and cholesterol in interstitial fluid simultaneously for prognosis of metabolic syndrome (MetS), a porous surface-enhanced Raman scattering (SERS) microneedle was fabricated by integrating SERS probes and three kinds of enzyme in three independent areas of one porous microneedle, respectively. Paraffin wax was used as a hydrophobic barrier to segment a porous microneedle to three independent areas, in which gold nanoshells (used as SERS substrates), 4-mercaptophenylboronic acid (4-MPBA), and one of glucose oxidase, urate oxidase, and cholesterol oxidase were integrated, respectively, and thus SERS probes altered SERS signals under the action of enzymes on their substrates. In vitro experiments demonstrated stable and accurate detection within the ranges of 0-15 mM glucose, 0-0.9 mM uric acid, and 0-9 mM cholesterol. Animal studies confirmed its feasibility for in vivo use. This porous SERS microneedle offers a minimally invasive and convenient solution for multiplex detection and point-of-care diagnosis in patients.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.