International Journal of Mass Spectrometry最新文献

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Influence of acceleration field and inlet temperature on ion yields of small molecules in atmospheric pressure MALDI 常压下加速场和入口温度对小分子离子产率的影响
IF 1.7 3区 化学
International Journal of Mass Spectrometry Pub Date : 2025-10-07 DOI: 10.1016/j.ijms.2025.117535
Eugene Moskovets
{"title":"Influence of acceleration field and inlet temperature on ion yields of small molecules in atmospheric pressure MALDI","authors":"Eugene Moskovets","doi":"10.1016/j.ijms.2025.117535","DOIUrl":"10.1016/j.ijms.2025.117535","url":null,"abstract":"<div><div>The optimization of the accelerating electric field near a target plate as well as temperature of the inlet capillary in a mass spectrometer is essential for achieving a high ion yield in atmospheric pressure matrix-assisted laser desorption ionization (AP/MALDI) and atmospheric pressure/laser desorption ionization (AP/LDI). The yields of protonated rhodamine 6G ions, protonated matrix ions (M + H)<sup>+</sup>, matrix radical ions (M•)<sup>+</sup>, as well as protonated reserpine ions were studied as a function of the inlet capillary temperature in Orbitrap and electric field applied across the inlet and MALDI target plate. The findings indicated that 2,5-dihydroxybenzoic acid (DHB) generated predominantly cation radicals (M•)<sup>+</sup> rather than protonated ions. This trend is not common for most MALDI matrices used for positive-mode MALDI-MS analysis. By changing capillary temperature, it has been found that the temperature trends observed for the (DHB + H)<sup>+</sup>/(DHB•)<sup>+</sup> ratios in both AP/MALDI and electrospray ionization (ESI) are similar. The ion yield dependence on the electric field strength in AP/MALDI as well as low-pressure MALDI sources proved to be more complex than that suggested in the previously developed model accounting for charge neutralization in the plume mitigated by the field-driven separation of opposite charges. The laser photoionization of the matrix molecules within a thin surface layer of a matrix microcrystal followed by thermally activated proton transfer from the matrix radical cation to adjacent matrix molecules has been discussed.</div></div>","PeriodicalId":338,"journal":{"name":"International Journal of Mass Spectrometry","volume":"519 ","pages":"Article 117535"},"PeriodicalIF":1.7,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single amino acid variation identification in high resolution tandem mass spectrometry data in bottom up proteomics 自底向上蛋白质组学中单氨基酸变异的高分辨率串联质谱鉴定
IF 1.7 3区 化学
International Journal of Mass Spectrometry Pub Date : 2025-10-01 DOI: 10.1016/j.ijms.2025.117532
Kishankumar Bhimani , Arina Peresadina , Karina Burmak , Kartik Joshi , Attila Kertesz-Farkas
{"title":"Single amino acid variation identification in high resolution tandem mass spectrometry data in bottom up proteomics","authors":"Kishankumar Bhimani ,&nbsp;Arina Peresadina ,&nbsp;Karina Burmak ,&nbsp;Kartik Joshi ,&nbsp;Attila Kertesz-Farkas","doi":"10.1016/j.ijms.2025.117532","DOIUrl":"10.1016/j.ijms.2025.117532","url":null,"abstract":"<div><div>Database-searching based precursor ion identification in tandem mass spectrometry data analysis is limited to the search space. Once a single amino acid variation (SAAV) or a modification is not included to the search space, then its observed spectra will not be annotated correctly. Several methods have been developed to identify and localize post-translational modifications (PTMs); however, few methods have been introduced to identify peptide sequences with amino acid mutations. Here, we present our approach to detect SAAVs, called SeVa (standing for <u>Se</u>quence <u>Va</u>riation). SeVa is based on the High-Resolution Exact P-Value (HR-XPV) method (doi:10.1002/pmic.202300145), which builds an exact empirical null distribution by implicitly scoring the spectra against all possible amino acid sequences in high-resolution fragmentation settings. SeVa extracts the amino acid sequence from HR-XPV, which produces the highest score. The SeVa peptides identified are subjected to a homology search against a proteome database containing shuffled decoy protein sequences. This step increases the sensitivity of the results and the decoy identifications can be used to estimate the FDR. We tested SeVa with two experimental datasets related to immunopeptidomics (PXD017407) and cancer (PDC000224), and our method identified 781 and 15,764 peptide sequences with mutations at 1.68% and 0.52% of FDRs.</div></div>","PeriodicalId":338,"journal":{"name":"International Journal of Mass Spectrometry","volume":"519 ","pages":"Article 117532"},"PeriodicalIF":1.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methods for a comprehensive targeted and suspect screen survey of exogenous chemicals in shed baby teeth 方法:对脱落乳牙外源性化学物质进行全面、有针对性和可疑的筛查调查
IF 1.7 3区 化学
International Journal of Mass Spectrometry Pub Date : 2025-09-27 DOI: 10.1016/j.ijms.2025.117534
Lynne P. Heilbrun , Anabel Rodriguez , L. Aubree Shay , David Gimeno Ruiz de Porras , Inkyu Han , Kristin Favela
{"title":"Methods for a comprehensive targeted and suspect screen survey of exogenous chemicals in shed baby teeth","authors":"Lynne P. Heilbrun ,&nbsp;Anabel Rodriguez ,&nbsp;L. Aubree Shay ,&nbsp;David Gimeno Ruiz de Porras ,&nbsp;Inkyu Han ,&nbsp;Kristin Favela","doi":"10.1016/j.ijms.2025.117534","DOIUrl":"10.1016/j.ijms.2025.117534","url":null,"abstract":"<div><h3>Background</h3><div>Semi-volatile organic chemicals (SVOCs) have been studied in shed baby teeth as a potential tool to identify environmental triggers of autism. However, these targeted studies are limited by the availability of standards, leaving a vast number of chemicals unexplored for autism risk.</div></div><div><h3>Objective</h3><div>We explored the use of two-dimensional gas chromatography time-of-flight mass spectrometry (GCxGC-TOFMS) as a viable and accurate method for identifying and quantifying a wide range of low-level SVOCs in deciduous teeth.</div></div><div><h3>Methods</h3><div>Tooth samples were derived from The Autism Tooth Fairy Study. Ground tooth samples (n = 44) were analyzed using GCxGC-TOFMS. Targeted and non-targeted analyses were performed. Targeted compounds were reviewed against reference spectra for identification, and non-targets were manually curated using the National Institute of Standards and Technology Library data, as available. Kovat's retention index was used for identifications without a reference standard.</div></div><div><h3>Results</h3><div>From the 44 tooth samples analyzed, 11,971 total endogenous and exogenous features were cataloged, including unknowns without a suitable library match. Of the 203 compounds detected, 106 high-fidelity matched compounds were identified spanning 45 subclasses of toxicants. Eighty-nine of these compounds are known developmental toxicants or endocrine disruptors. The remaining compounds were toxicants with acute or chronic toxicity data but had no developmental or endocrine-disrupting activity data.</div></div><div><h3>Conclusion</h3><div>This study demonstrates the utility of GCxGC-TOFMS for detecting a wide range of SVOCs in children's teeth, identifying a substantial number of developmental toxicants and other toxic chemicals. The detection of these compounds highlights critical gaps in our understanding of children's chemical exposures.</div></div>","PeriodicalId":338,"journal":{"name":"International Journal of Mass Spectrometry","volume":"519 ","pages":"Article 117534"},"PeriodicalIF":1.7,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a laser desorption ionization – ion mobility – mass spectrometry method to accelerate the cyclic peptides stereochemistry determination workflow 建立了一种激光解吸电离-离子迁移-质谱法,以加快环多肽立体化学的测定工作流程
IF 1.7 3区 化学
International Journal of Mass Spectrometry Pub Date : 2025-09-23 DOI: 10.1016/j.ijms.2025.117533
Jia-Xuan Yan, Wendy Zhong
{"title":"Development of a laser desorption ionization – ion mobility – mass spectrometry method to accelerate the cyclic peptides stereochemistry determination workflow","authors":"Jia-Xuan Yan,&nbsp;Wendy Zhong","doi":"10.1016/j.ijms.2025.117533","DOIUrl":"10.1016/j.ijms.2025.117533","url":null,"abstract":"<div><div>Cyclic peptides are a class of compounds with significant therapeutic potential. The increase in number of stereocenters resulting in exponential surge of stereoisomers poses an immense challenge on the stereochemical analysis of these molecules. Current stereochemistry control strategies for synthetic peptides requires chiral LC method development using synthetic markers. However, the synthesis of all possible stereoisomers as well as chiral LC method development to separate all of them is extremely costly and time consuming. Alternatively, cyclic peptides can be hydrolyzed into single amino acids (AA), further derivatized by chiral reagents for LC-MS analysis, and compared to AA standards subjected to identical derivatization protocols. This LC-MS based methodologies could determine chirality for all possible stereoisomers within short time (less than 1hr) avoiding the need to synthesize large number of cyclic peptide stereoisomers. While examining literature reported LC-MS methodologies, we sought opportunities to further reduce the analysis time required for comprehensive determinations of the stereochemistry of cyclic peptides via advanced MS platforms. Here-in we report a new laser desorption/ionization-ion mobility-mass spectrometry (LDI-IM-MS) method for the rapid determination of amino acid stereochemistry in cyclic peptides. Chiral derivatization reagents, <span>l</span>-FDLA (Nα-(2,4-dinitro-5-fluorophenyl)-<span>l</span>-leucinamide) and <span>d</span>-FDLA were used to derivatize the amino acids into diastereomer pairs. The diastereomers were differentiated using LDI-IM-MS on a Bruker timsTOF flex platform, with key ion mobility parameters optimized and experimental CCS (collision cross section) values calculated. Enhanced sample preparation workflow including solid phase extraction (SPE) and CuCl<sub>2</sub> doping exhibited better ion mobility differentiation for selected samples. This approach was successfully applied to the analysis of polymyxin B, a natural product cyclic peptide, providing comprehensive stereochemical determination of all constituent amino acids within 1 min. The new workflow not only accelerates the stereochemical analysis of cyclic peptides but also holds promise for broader applications in pharmaceuticals including chiral quality control and monitoring of peptide stability.</div></div>","PeriodicalId":338,"journal":{"name":"International Journal of Mass Spectrometry","volume":"519 ","pages":"Article 117533"},"PeriodicalIF":1.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the results of the aberration approach for the calculation of electrostatic TOF mass analyzers with simulation in SIMION 用像差法计算静电TOF质量分析仪与SIMION仿真结果的比较
IF 1.7 3区 化学
International Journal of Mass Spectrometry Pub Date : 2025-09-10 DOI: 10.1016/j.ijms.2025.117526
Daniil D. Odintsov , Sergey S. Poteshin
{"title":"Comparison of the results of the aberration approach for the calculation of electrostatic TOF mass analyzers with simulation in SIMION","authors":"Daniil D. Odintsov ,&nbsp;Sergey S. Poteshin","doi":"10.1016/j.ijms.2025.117526","DOIUrl":"10.1016/j.ijms.2025.117526","url":null,"abstract":"<div><div>In the last few years, high-resolution time-of-flight mass analyzers (TOF-MA) have been intensively developed. Their development requires tools that provide very accurate calculation of ion trajectories in electric fields, since an error on the scale of hundreds of picoseconds at millisecond flight times (10<sup>−10</sup> %) already leads to noticeable distortions of the result. The main apparatus for calculating such systems is the aberration theory, but it has a limitation in the accuracy of calculations, since it is based on expansion in a series, and taking into account the next order of expansion significantly complicates the formulas. In this regard, using the example of calculating ion trajectories for TOF-MA with sector electrostatic fields, a comparison of the results, obtained using the aberration theory was carried out with the results of modeling in Simion. Results of the comparison indicate the possibility of applicability of the program for calculating multi-turn mass analyzers (MT-MA). Using the developed program, MT-MA with a resolution of 470,000 was calculated.</div></div>","PeriodicalId":338,"journal":{"name":"International Journal of Mass Spectrometry","volume":"519 ","pages":"Article 117526"},"PeriodicalIF":1.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
GC-MS unveils monosaccharide D-Allose in Terminalia arjuna bark extract acting against biofilm regulatory proteins (SrtA and SarA) of Staphylococcus aureus: A drug discovery approach GC-MS揭示了苦参树皮提取物中单糖D-Allose对金黄色葡萄球菌生物膜调控蛋白(SrtA和SarA)的作用:一种药物发现方法
IF 1.7 3区 化学
International Journal of Mass Spectrometry Pub Date : 2025-09-06 DOI: 10.1016/j.ijms.2025.117527
Shraavani Konatam, Dijendra Nath Roy
{"title":"GC-MS unveils monosaccharide D-Allose in Terminalia arjuna bark extract acting against biofilm regulatory proteins (SrtA and SarA) of Staphylococcus aureus: A drug discovery approach","authors":"Shraavani Konatam,&nbsp;Dijendra Nath Roy","doi":"10.1016/j.ijms.2025.117527","DOIUrl":"10.1016/j.ijms.2025.117527","url":null,"abstract":"<div><div>The rising prevalence of biofilm-related infections and foodborne diseases, particularly those caused by <em>Staphylococcus aureus</em>, presents significant challenges to conventional treatment strategies due to the increasing phenomenon of antibiotic resistance caused by biofilm. In response, this study employed GC-MS analysis of the methanolic and n-hexane extracts of <em>Terminalia arjuna</em> bark, a traditional medicinal plant, and identified a diverse array of bioactive compounds. All 79 compounds identified from GC-MS data were examined to determine the best binding score with SrtA and SarA, two key proteins involved in the development of biofilms and the pathogenicity of <em>Staphylococcus aureus.</em> Among 79 compounds, D-Allose, a monosaccharide, demonstrated the best binding affinity with SrtA (−6.304 kcal/mol) by involving five hydrogen bonds and SarA (−5.101 kcal/mol) by involving three hydrogen bonds, compared to other compounds among 158 interactions. Molecular dynamics and simulations were performed for 100 ns to analyze the interaction parameters with SrtA and SarA. Corresponding MM-GBSA binding energy values were −33.66 kcal/mol (D-Allose with SrtA), −18.09 kcal/mol (D-Allose with SarA). The fact that D-Allose has zero violations of Lipinski's Rule of Five further exemplifies its suitability as a medication candidate. These interactions suggest that D-Allose may interfere with bacterial adhesion, quorum sensing, and the synthesis of the biofilm matrix by interacting with SrtA and SarA. To the best of our knowledge, this is the first study to elucidate the potential mechanism by which D-Allose exerts its antibiofilm activity. Future research on in vitro and <em>in vivo</em> studies should focus on experimental validation to establish D-Allose as a promising monosaccharide candidate for combating biofilm-related antibiotic-resistant <em>Staphylococcus aureus</em> infections.</div></div>","PeriodicalId":338,"journal":{"name":"International Journal of Mass Spectrometry","volume":"518 ","pages":"Article 117527"},"PeriodicalIF":1.7,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Two-dimensional tandem mass spectrometry in a linear quadrupole ion trap using consecutive product ion sweeps 二维串联质谱法在线性四极离子阱使用连续产品离子扫描
IF 1.7 3区 化学
International Journal of Mass Spectrometry Pub Date : 2025-09-05 DOI: 10.1016/j.ijms.2025.117524
Eric T. Dziekonski
{"title":"Two-dimensional tandem mass spectrometry in a linear quadrupole ion trap using consecutive product ion sweeps","authors":"Eric T. Dziekonski","doi":"10.1016/j.ijms.2025.117524","DOIUrl":"10.1016/j.ijms.2025.117524","url":null,"abstract":"<div><div>Two-dimensional tandem mass spectrometry (2D-MS/MS) is a powerful analysis technique which can quickly produce a map of all precursor-product ion relationships within a sample, from a single ion injection event, and is greatly suited to low-cost instrumentation like ion traps. The ability to record this type of information in an unsupervised and unbiased manner makes this technique highly amenable to artificial intelligence-based workflows which can statistically examine the datasets to perform structural elucidation, generate more confident peak assignments, and/or discover new chemistries. Previous implementations of the 2D-MS/MS scan function kept the RF voltage applied to the trap constant and scanned an auxiliary frequency to eject and detect the product ions. A direct consequence of this is that ions of higher precursor and product <em>m/z</em> would have a lower observed mass resolution and sensitivity, leading to more potential candidates for peak identification algorithms and higher uncertainty in the reported results. Herein, we describe a new approach to 2D-MS/MS analysis in a linear quadrupole ion trap which utilizes consecutive, traditional, RF amplitude ramps to resonantly eject ions from the trap near the boundary of the Mathieu stability diagram. Relative to its predecessors, the technique does require more time to acquire, however, it necessitates fewer instrument modifications and has more robust calibration procedures. More importantly though, its use produces higher sensitivity spectra with constant 2D peak resolutions (3 Da FWHM @ 125 kDa/s), which can be predicted based on the set product ion scan rate and resonant ejection point. Additional discussion is aimed at methods by which the scan might be improved, or optimized, for higher speed workflows utilizing sample arrays. This work paves the path towards isotopically resolved 2D-MS/MS spectra, which would greatly enhance the power of the technique.</div></div>","PeriodicalId":338,"journal":{"name":"International Journal of Mass Spectrometry","volume":"518 ","pages":"Article 117524"},"PeriodicalIF":1.7,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145026637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structures of species formed by sequential methane activation by Ta+: Infrared multiple photon dissociation spectroscopy and ab Initio calculations 序列甲烷活化的物质结构:红外多光子解离光谱和从头计算
IF 1.7 3区 化学
International Journal of Mass Spectrometry Pub Date : 2025-09-03 DOI: 10.1016/j.ijms.2025.117525
P.B. Armentrout , Bastiaan Poetsma , David H. Loertscher , Satish Kumar , Joost M. Bakker
{"title":"Structures of species formed by sequential methane activation by Ta+: Infrared multiple photon dissociation spectroscopy and ab Initio calculations","authors":"P.B. Armentrout ,&nbsp;Bastiaan Poetsma ,&nbsp;David H. Loertscher ,&nbsp;Satish Kumar ,&nbsp;Joost M. Bakker","doi":"10.1016/j.ijms.2025.117525","DOIUrl":"10.1016/j.ijms.2025.117525","url":null,"abstract":"<div><div>Products resulting from the sequential activation of four and five methane molecules by atomic tantalum cations were characterized by gas-phase infrared multiple photon dissociation spectroscopy and density functional theory (DFT) calculations. Tantalum cations were generated using a laser ablation source and reacted with methane in a linear radiofrequency ion trap before mass analysis and spectroscopic interrogation in a Fourier transform ion cyclotron resonance mass spectrometer coupled to the free-electron laser for intracavity experiments (FELICE) beamline. Product ions were irradiated using infrared light over the 300−2000 cm<sup>−1</sup> spectral range. Comparisons between the experimental and DFT-calculated spectra enabled structural determination of the products formed. The observed products are (CH<sub>3</sub>)<sub>2</sub>TaC<sub>2</sub>H<sub>2</sub><sup>+</sup> and (CH<sub>3</sub>)<sub>3</sub>Ta(C<sub>2</sub>H<sub>3</sub>)<sup>+</sup>. Formation of these products provides evidence for efficient C−H bond activation and subsequent C−C coupling on the atomic tantalum cation.</div></div>","PeriodicalId":338,"journal":{"name":"International Journal of Mass Spectrometry","volume":"518 ","pages":"Article 117525"},"PeriodicalIF":1.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A thermal perspective on MALDI ionization: A long journey from fundamental mechanisms to a practical solution for carbohydrate analysis MALDI电离的热视角:从基本机制到碳水化合物分析的实际解决方案的漫长旅程
IF 1.7 3区 化学
International Journal of Mass Spectrometry Pub Date : 2025-08-30 DOI: 10.1016/j.ijms.2025.117516
I-Chung Lu
{"title":"A thermal perspective on MALDI ionization: A long journey from fundamental mechanisms to a practical solution for carbohydrate analysis","authors":"I-Chung Lu","doi":"10.1016/j.ijms.2025.117516","DOIUrl":"10.1016/j.ijms.2025.117516","url":null,"abstract":"<div><div>Matrix-assisted laser desorption/ionization (MALDI) is a vital analytical tool, but the fundamental ionization mechanism has long been debated. This perspective summarizes over a decade of research to introduce a comprehensive thermal framework, culminating in the quantitative thermally induced proton transfer (TIPT) model for MALDI. Validated by numerous experiments without fitting parameters, we identify that laser-induced temperature is the dominant factor in the primary ionization of protonated ions. The principle was subsequently applied to resolve the longstanding challenges of carbohydrate analysis. To overcome the characteristically low sensitivity of carbohydrates, the rapidly freeze-drying droplet (RFDD) sample preparation method was developed by preserving homogeneously distributed, preformed sodium adducts. RFDD increases ion intensity by over two orders of magnitude, eliminates the \"sweet spot\" effect, and enables reliable quantitative analysis. This journey from fundamental mechanistic inquiry to a practical, innovative technology powerfully illustrates how basic science drives analytical advancements.</div></div>","PeriodicalId":338,"journal":{"name":"International Journal of Mass Spectrometry","volume":"518 ","pages":"Article 117516"},"PeriodicalIF":1.7,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144925849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Extended m/z range of coaxial linear ion trap/time-of-flight mass spectrometry using dynamic electric field ejection 使用动态电场喷射的同轴线性离子阱/飞行时间质谱扩展m/z范围
IF 1.7 3区 化学
International Journal of Mass Spectrometry Pub Date : 2025-08-30 DOI: 10.1016/j.ijms.2025.117517
Yixue Cao , Xuesong Zhang , Jilong Wang , Chenxin Wu , Lei Hua , Ping Chen , Haiyang Li
{"title":"Extended m/z range of coaxial linear ion trap/time-of-flight mass spectrometry using dynamic electric field ejection","authors":"Yixue Cao ,&nbsp;Xuesong Zhang ,&nbsp;Jilong Wang ,&nbsp;Chenxin Wu ,&nbsp;Lei Hua ,&nbsp;Ping Chen ,&nbsp;Haiyang Li","doi":"10.1016/j.ijms.2025.117517","DOIUrl":"10.1016/j.ijms.2025.117517","url":null,"abstract":"<div><div>Linear ion trap/time-of-flight mass spectrometry (LIT/TOFMS) to perform ion accumulation in a separate LIT prior to mass analysis is a more generally accepted method to improve duty cycle. However, a long distance between the accumulation region and the TOF pusher led to time-of-flight dispersion of the injected ions with different <em>m/z</em>, thereby limiting the detectable mass range in a single scan. This paper studies the characteristic behaviors of ion ejection from a segmented LIT through SIMION simulation. A novel dynamic electric field ejection strategy is proposed to broaden the ejection mass range of LIT. By linearly scanning the voltages on the segmented LIT and the ion gate, the axial potential distribution is dynamically adjusted to gradually shift the ion accumulation region toward the TOF pusher. As a result, the ejection distance between the accumulation region and the TOF pusher was slowly shortened, reducing time dispersion among different <em>m/z</em> ions. The simulation results demonstrate that this method significantly improves mass range of the coaxial dual LIT/TOFMS, achieving near 100 % injection efficiency for <em>m/z</em> 130–2000 with a mass window ratio <em>(m/z)</em><sub><em>max</em></sub><em>/(m/z)</em><sub><em>min</em></sub> of 15. This technology provides a solution for broadening the mass range of LIT-based hybrid instruments.</div></div>","PeriodicalId":338,"journal":{"name":"International Journal of Mass Spectrometry","volume":"518 ","pages":"Article 117517"},"PeriodicalIF":1.7,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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