MALDI-TOF-MS unveils the distribution of oligosaccharides produced by hydrolysis of lichen polysaccharides through acidic and oxidative methods – a comparative study

IF 1.6 3区化学 Q3 PHYSICS, ATOMIC, MOLECULAR & CHEMICAL

International Journal of Mass Spectrometry Pub Date : 2025-06-06 DOI:10.1016/j.ijms.2025.117473

Camille Guitteny , Simon Ollivier , Oznur Yeni , Mathis Ralaivao , Mathieu Fanuel , Joël Boustie , Isabelle Compagnon , Vincent Ferrières , Solenn Ferron , Hélène Rogniaux , David Ropartz , Laurent Legentil , Françoise Le Dévéhat

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Abstract

MALDI-TOF MS methods coupled with offline chromatographic data were used to compare the distribution of oligosaccharides generated from acidic or oxidative degradation (Fitdog) of high molecular weight polysaccharides (>10 kDa) obtained from two lichens Lasallia pustulata and Cetraria islandica. MALDI allowed to quickly compare the kinetics of degradation on both models (starting from non-purified polysaccharides) and to evaluate the dispersity of the resulting oligosaccharides. MALDI-MS confirmed on one hand that TFA hydrolysis gave neutral oligosaccharides easy to correlate with the chemical formula. On the other hand, more structural diversity was evidenced using the Fitdog protocol. Deep analysis of the MALDI data highlighted the formation of by-products corresponding to modified oligosaccharides (e.g., intracyclic cleavages).

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MALDI-TOF-MS揭示了通过酸性和氧化方法水解地衣多糖产生的低聚糖的分布-一项比较研究

采用MALDI-TOF质谱法结合离线色谱数据，比较了从两种地衣中获得的高分子量（>10 kDa） Lasallia pustulata和Cetraria islandica中酸性或氧化降解（Fitdog）产生的低聚糖的分布。MALDI可以快速比较两种模型（从未纯化的多糖开始）的降解动力学，并评估所得低聚糖的分散性。MALDI-MS一方面证实了TFA水解得到的中性低聚糖易于与化学式相关联。另一方面，使用Fitdog协议证明了更多的结构多样性。对MALDI数据的深入分析强调了与改性低聚糖（例如，环内裂解）相对应的副产物的形成。

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来源期刊

International Journal of Mass Spectrometry 物理-光谱学

CiteScore

3.60

自引率

5.60%

发文量

145

审稿时长

71 days

期刊介绍： The journal invites papers that advance the field of mass spectrometry by exploring fundamental aspects of ion processes using both the experimental and theoretical approaches, developing new instrumentation and experimental strategies for chemical analysis using mass spectrometry, developing new computational strategies for data interpretation and integration, reporting new applications of mass spectrometry and hyphenated techniques in biology, chemistry, geology, and physics. Papers, in which standard mass spectrometry techniques are used for analysis will not be considered. IJMS publishes full-length articles, short communications, reviews, and feature articles including young scientist features.