化学最新文献

{"title":"Paper-based electrochemical aptasensor using Ti₃C₂ MXene for sensitive detection of Mammaglobin-A biomarker.","authors":"Jaydeep Kanungo, Somya Sadaf, Dakshita Snud Sharma, Dharitri Rath, Chinmay Ghoroi","doi":"10.1016/j.talanta.2026.130421","DOIUrl":"10.1016/j.talanta.2026.130421","url":null,"abstract":"<p><p>Breast cancer is one of the most prevalent cancers worldwide and a leading cause of cancer-related mortality. However, early detection of the disease can significantly reduce the mortality rate of cancer patients. The current detection methods of breast cancer are expensive, time consuming, invasive and require professional assistance. To overcome these challenges, a minimally invasive low-cost paper-based point-of-care (POC) electrochemical sensing platform has been developed for early-stage detection of breast cancer. The proposed sensor is fabricated using a screen-printing technique, incorporating two-dimensional Ti₃C₂ (MXene) nanosheets onto the working electrode. These nanosheets provide enhanced electrochemical performance, enabling an exceptionally low limit of detection (LOD) ∼ 4 cells mL^-1) attributed to their high conductivity and large surface area. The electrode surface is further functionalized with specific aptamers, facilitating selective target recognition. The proof-of-concept study was validated using multiple cell lines, including A549 (lung cancer cells), NIH3T3 (fibroblast), and HEK293 (kidney cells), and MDA-MB-231(breast cancer cell line), to evaluate the specificity of the developed aptasensor. The sensing mechanism relies on the selective binding of aptamers to Mammaglobin A proteins expressed on circulating tumor cells (CTCs). This interaction induces conformational changes in the aptamer, resulting in measurable alterations in the electrochemical properties at the electrode interface. The conjugation of the biomarker with the aptamer upon aptamer-protein interaction, results in a change in current, which is sensed by cyclic voltammogram (CV). The resulting signal is used to plot a calibration curve for quantitative analysis. Further, these sensing experiments are validated using in vitro experiments with human blood serum as a proof of concept. Overall, the developed paper-based electrochemical POC device offers significant advantages, including affordability, ease of use, rapid response, and quantitative detection capability.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130421"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148710884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to 'Intercalating of AIEgens into MoS₂ nanosheets to induce crystal phase transform for enhanced photothermal and photodynamic synergetic anti-tumor therapy' [Talanta, 271 (2024) 125677].","authors":"Jiaxin Li, Yanzhi Xing, Xuwei Chen","doi":"10.1016/j.talanta.2026.130634","DOIUrl":"10.1016/j.talanta.2026.130634","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130634"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149007641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Split aptamer-regulated AuNF nanozymes for colorimetric and electrochemical dual-readout kanamycin biosensing.","authors":"Hailiang Qin, Xinyan Guo, Yao Zhao, Meng Luo, Minqiao Feng, Haoqian Xue, Pengbo Zhang, Meilin Zhou, Na Wang, Shuxia Xu","doi":"10.1016/j.talanta.2026.130380","DOIUrl":"10.1016/j.talanta.2026.130380","url":null,"abstract":"<p><p>Kanamycin (Kana) residues released into aquatic environments due to its extensive use have raised increasing concerns because of their potential risks to ecosystems and human health. In this work, we report a label-free split aptamer-modified gold nanoflower (AuNF)-based aptasensing platform with colorimetric and electrochemical readouts for the determination of Kana in water. Two thiolated split aptamer fragments (SPA1 and SPA2) were immobilized on AuNFs through Au-S bonds. The flower-like AuNFs provided a high-surface-area scaffold for aptamer loading, while the DNA modification further enhanced the peroxidase-like activity of AuNFs toward TMB oxidation. In the presence of Kana, target-induced assembly of SPA1 and SPA2 promoted the aggregation of AuNFs, which inhibited their catalytic activity toward TMB oxidation, thereby reducing oxTMB formation and the resulting colorimetric and electrochemical signals. Based on this signal-off mechanism, the platform enabled quantitative detection of Kana with linear ranges of 100-3000 nM for colorimetry and 50-2000 nM for chronoamperometry, with limits of detection of 0.51 and 0.074 nM, respectively. The method also showed satisfactory selectivity, reproducibility, and storage stability. In spiked environmental water samples, recoveries ranged from 87.7% to 106.2% with relative standard deviations below 5.0%, and the electrochemical results were in acceptable agreement with those obtained by HPLC-MS. This study provides a simple and effective dual-readout aptasensing strategy for Kana determination in environmental water samples.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130380"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148710936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-modal immunoassay for prostate cancer-derived exosomes enabled by a black phosphorus/silver SERS substrate and Yb/Er co-doped NaGdF₄ UCL nanoprobes.","authors":"Yasheng Dai, Jie Yao, Shiyuan Wang, Changgan Lai, Jie Gong, Chenjie Gu, Tao Jiang, Kerong Wu","doi":"10.1016/j.talanta.2026.130403","DOIUrl":"10.1016/j.talanta.2026.130403","url":null,"abstract":"<p><p>Exosomes are regarded as cancer-associated biomarkers for early diagnosis and prognosis in clinical bioassays. Nevertheless, it remains a challenge to develop an approach that can achieve ultra-sensitive and quantitative detection of exosomes. Herein, an innovative dual-mode immunoassay dependent on surface-enhanced Raman scattering (SERS) and upconversion luminescence (UCL) was developed to detect prostate cancer-derived exosomes. A novel SERS-active immunosubstrate was constructed by combining black phosphorus (BP) with Ag nanoparticles, which exhibited an enhancement factor (EF) of about 3.69 × 10⁹, due to the synergistic electromagnetic and chemical effects. Meanwhile, an immunoprobe with stable and visually distinguishable UCL was developed based on Yb/Er co-doped NaGdF₄ nanoparticles with an intrinsic two-photon process. The specific capture of exosomes was achieved through antigen-antibody binding within the sandwich immunostructure, achieving limits of detection (LOD) as low as 93 and 172 particles/mL based on the dual-modal detection, respectively. Furthermore, the recognition of different derived exosomes was simply realized by visually observing the green fluorescence with different intensities, which paves the way for efficient preliminary identification of prostate cancer. Finally, the validation with clinical serum samples proved that the proposed approach holds significant promise for rapid cancer screening and diagnosis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130403"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AuNP decorated-Ni-MOF nanosheet based cDNA sensor for detection of miRNA-128 in Alzheimer's disease.","authors":"Sonia Rani, Karthik Vinay Mahesh, Rajat Sandhir, Nirmal Prabhakar","doi":"10.1016/j.talanta.2026.130405","DOIUrl":"10.1016/j.talanta.2026.130405","url":null,"abstract":"<p><p>A cDNA sensor based on gold nanoparticles (Au) supported on nickel metal-organic framework nanosheets (MOF-NS) modified fluorine-tin oxide (FTO) glass electrode for the ultrasensitive and selective detection of miRNA-128 biomarker associated with the progression of Alzheimer's disease (AD) has been reported. The MOF-NS and Au nanoparticles were electrodeposited onto FTO electrode using chronoamperometry to immobilize 5'-biotinylated DNA (btn-cDNA) complementary to miRNA-128 by streptavidin-biotin interaction, generating the modified electrode (btn-cDNA/SV/Au/MOF-NS/FTO). The fabricated electrode was characterized using various surface characterization techniques, including FESEM, XPS, FTIR, and electrochemical methods. The fabricated electrode was then utilized for the selective detection of miRNA-128 using electrochemical impedance spectroscopy. The results indicated a linear response range of 1.0 × 10^-1 fM-1.0 × 10² nM with limit of detection of 0.017 fM, and sensitivity of 180.11 Ω fM^-1cm^-2. Moreover, the cDNA sensor showed satisfactory performance with real serum samples from AD patients and healthy individuals, as validated by real time-PCR technique with area under curve of 0.93 and a sensitivity of 90%, respectively, demonstrating the potential of present cDNA sensing approach in the biomedical field.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130405"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Development of a novel selective extraction system for lithium and application to its secondary resources\" [Talanta, 2026, Article 130545].","authors":"Vatsal Trivedi, Sai Krishna Devulapally, R Shekhar, G Venkateswarlu","doi":"10.1016/j.talanta.2026.130658","DOIUrl":"10.1016/j.talanta.2026.130658","url":null,"abstract":"","PeriodicalId":435,"journal":{"name":"Talanta","volume":" ","pages":"130658"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149069015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aza-Paternò-Büchi derivatization enables annotation of triacylglycerols by negative-ion-mode mass spectrometry at the double bond level.","authors":"Andrea Cerrato, Aldo Laganà, Carmela Maria Montone, Enrico Taglioni, Daniel Weissman, Anna Laura Capriotti","doi":"10.1016/j.talanta.2026.130396","DOIUrl":"10.1016/j.talanta.2026.130396","url":null,"abstract":"<p><p>Triacylglycerols (TGs) represent one of the most abundant lipid classes in biological and food matrices; however, their structural characterization by mass spectrometry remains challenging because conventional workflows often provide only sum composition information. In the present study, an aza-Paternò-Büchi (aPB) derivatization strategy based on 6-azauracil was optimized and applied for negative-ion-mode, isomer-resolved analysis of TGs by high-resolution mass spectrometry. Reaction conditions were systematically investigated through solvent optimization, mixture design modeling, and kinetic studies. Moreover, the ionization and fragmentation behavior of aPB-derivatized TGs was comprehensively investigated, revealing distinct fragmentation pathways associated with charge localization effects in neutral lipids. Application to complex matrices demonstrated the feasibility of the approach, providing direct access to TG regioisomer distributions in human plasma and vegetable seed oils. Overall, the proposed strategy enables untargeted and double-bond-resolved annotation of TGs in complex matrices while maintaining straightforward sample preparation and compatibility with routine lipidomics workflows. For the first time, negative-ion mode was employed for TG annotation, expanding the molecular information accessible in TG lipidomics and food analysis.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130396"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148710953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid determination of Ru, Rh, Pd, Ir, and Pt in rock samples using ICP-MS following microwave digestion.","authors":"Klimentsi Cherviakouski, Mohammed Khalid, Ma'an Amad","doi":"10.1016/j.talanta.2026.130321","DOIUrl":"10.1016/j.talanta.2026.130321","url":null,"abstract":"<p><p>This study introduces a novel rapid and efficient analytical approach for the determination of PGEs, excluding Os, in rock samples by ICP-MS. This approach combines effective matrix removal in silicate rock samples through the formation of insoluble fluorides during microwave-assisted digestion and the volatilization of silicon as SiF₄ with the capability of ICP-MS to quantify target isotopes at very low signal intensities (10-100 cps) using optimized acquisition parameters. Moreover, the ability to perform ICP-MS measurements at such low signal intensities also enables matrix reduction in other sample types, such as ores, through simple dilution while maintaining sufficient analytical sensitivity. Since proposed strategy employs fast microwave dissolution of PGEs together with their reliable and accurate determination, without the need for complex and time-consuming matrix-separation procedures such as ion-exchange chromatography or tellurium co-precipitation, the analytical workflow is significantly simplified, and the overall analysis time is reduced several-fold compared with conventional methods. At the same time, detection and quantitation limits are below 0.1 and 0.5 ng/g, respectively, for all analytes except Pd, for which the corresponding limits are 0.18 and 0.9 ng/g, enabling the determination of PGEs in variety rock types. The new method was verified using six rocks certified reference materials (CRMs) MUH-1 (harzburgite), NCS-DC73354/GBW07290 (peridotite), OKUM (komatiite), and UMT-1 (ultramafic ore tailings), WMS-1a (massive sulfide) and NCS-DC73356/GBW07292 (chromitite). The results obtained showed excellent agreement with both certified values and previously published data, with the vast majority of measurements falling within stated uncertainties or within ±10% of certified values.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130321"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148688414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput single-cell metabolomics to reveal the hepatotoxicity of 77PDQ exposure in mice.","authors":"Ning Liu, Haishen Zeng, Junhao Lin, Jiewei Deng, Chunyan Hao, Tiangang Luan, Yunyun Yang","doi":"10.1016/j.talanta.2026.130393","DOIUrl":"10.1016/j.talanta.2026.130393","url":null,"abstract":"<p><p>Elucidating the effects of pollutants at the level of primary single cells is of great significance for an in-depth understanding of their health risks and toxicological mechanisms. N,N'-bis (1,4-dimethylpentyl)-p-phenylenediamine quinone (77PDQ) is an emerging environmental contaminant with multiple biological toxicities; however, its toxic effects at the primary single-cell level remain unexplored. In this study, we established a high-throughput single-cell metabolomics platform based on label-free CyESI-MS and applied it to the analysis of freshly isolated primary mouse liver cells for toxicological assessment of 77PDQ exposure at single-cell resolution. This strategy enabled us to capture cell-to-cell metabolic heterogeneity that was lost in conventional bulk measurements. Analysis of primary mouse liver cells revealed 181 significantly altered metabolites (|Log₂FC|>1, p < 0.05) following 77PDQ exposure. Pathway analysis demonstrated substantial disruptions in glycerophospholipid, arginine-proline, and beta-alanine metabolism. Eight lipid biomarkers (PC (41:6), PS (31:4), Cer (d41:1), TAG (53:2), LPG (18:1), SM (d42:3), FA (23:0), PA (36:2)) showed excellent diagnostic capacity (AUC>0.9). Our study provides the single-cell metabolomics evidence of 77PDQ hepatotoxicity and offers new mechanistic insights into this emerging environmental contaminant.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130393"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148676610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural modification of ZnO@Ag₂O nanostructures functionalized on optical fiber for room-temperature acetone detection with sub-ppm sensitivity.","authors":"Ashok K Sahu, Kanhu Ch Nayak, Sukanta K Tripathy","doi":"10.1016/j.talanta.2026.130425","DOIUrl":"10.1016/j.talanta.2026.130425","url":null,"abstract":"<p><p>In this work, a high-performance, room-temperature-operated acetone gas sensor is developed based on cladding-modified optical fiber functionalized with hierarchical flower-like ZnO@Ag₂O nanostructures. Unlike conventional metal-oxide gas sensors operating at elevated temperatures, the formation of a p-n heterojunction between p-type Ag₂O and n-type ZnO facilitates efficient charge separation and significantly lowers the activation energy for acetone oxidation, enabling enhanced sensing at ambient conditions. The structural and morphological properties were characterized by X-ray diffraction, FESEM, XPS, and FTIR. The sensing performance was achieved by coating the modified fiber cladding with ZnO@Ag₂O, where evanescent-field interaction and adsorption-induced optical property changes govern the sensing mechanism, as validated by COMSOL Multiphysics simulations. The flower-like morphology provides a larger surface area and abundant active sites, resulting in superior sensing performance compared to pristine ZnO nps. The performance parameters of the developed sensor include a sensitivity of 11.37/ppm, a limit of detection of 0.85 ppm, and response and recovery times of 14 s and 60 s, respectively, compared to pristine ZnO nps. Additionally, the sensor exhibits good selectivity for acetone over different interfering VOC species. Owing to its spark-free operation, low power consumption, and immunity to electromagnetic interference, the proposed sensing platform presents a promising solution for real-time acetone detection in industrial process monitoring and non-invasive biomedical diagnostics.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"312 Pt A","pages":"130425"},"PeriodicalIF":6.7,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148704793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}