Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy最新文献

{"title":"Development of a hemicyanine-based turn-on probe for MAO-A imaging in metabolic disorders in vivo and in serum","authors":"Lu Fan ,&nbsp;Yulong Jin ,&nbsp;Aiguo Zhang ,&nbsp;Youzhen Feng ,&nbsp;Kai Wang","doi":"10.1016/j.saa.2025.126736","DOIUrl":"10.1016/j.saa.2025.126736","url":null,"abstract":"<div><div>In this study, A fluorescent/photoacoustic dual-modal probe HD-MAO for hemicyanine derivatives was successfully designed and synthesized, achieving high-sensitivity and high-specificity detection of monoamine oxidase A (MAO-A). Through the ingenious combination of the specific MAO-A recognition group and the hemipanocyanine fluorophore, the probe produced a significant enhancement of the intramolecular charge transfer (ICT) effect after enzymatic digestion, showing the synergistic activation characteristics of fluorescence (∼730 nm) and photoacoustic (∼700 nm) signals, with a detection limit of 0.806 mg/L. In the diet-induced obese mouse model, HD-MAO successfully revealed a significant increase in liver MAO-A activity, which was highly consistent with the serum test results, providing molecular imaging evidence for the early diagnosis of metabolic diseases. The probe behaves stably under physiological conditions and shows no cross-reaction to common interferents. Serum detection experiments further confirm its clinical applicability. This study not only provides an innovative tool for the research on MAO-A-related mechanisms, but also opens up a new approach for the early diagnosis and treatment monitoring of metabolic diseases.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"344 ","pages":"Article 126736"},"PeriodicalIF":4.3,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144704822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of the biomolecular composition of skeletal muscle fibres affected by different types of pathology using by FTIR microspectroscopy","authors":"Paula Kasprzyk ,&nbsp;Joanna Dudała ,&nbsp;Marek Lankosz ,&nbsp;Dariusz Adamek ,&nbsp;Borys Kwinta ,&nbsp;Edyta Radwańska ,&nbsp;Joannis Lekkas ,&nbsp;Gianfelice Cinque ,&nbsp;Magdalena Szczerbowska-Boruchowska","doi":"10.1016/j.saa.2025.126727","DOIUrl":"10.1016/j.saa.2025.126727","url":null,"abstract":"<div><div>Myopathies, a group of muscle disorders with varied etiologies, cause significant alterations in muscle tissue biomolecular composition. Accurate differentiation of myopathies is essential for effective diagnosis, treatment and prognosis. Fourier-transform infrared microspectroscopy (microFTIR) is a valuable, non-destructive method for analysing the main biological macromolecules at the microscopic level. Therefore, it may offer insight into the investigation of both primary and secondary myopathies. In our work we use microFTIR to identify unique spectral markers associated with disease progression. For this purpose, muscle tissues from patients diagnosed with dystrophy and myopathy, as well as control tissues, were subjected to probing. The results reveal significant differences in the distribution of lipid, protein, and nucleic acid absorption bands among the sample groups, particularly in regions associated with muscle fibre and connective tissue structure. Notably, vibrational bands at ∼1043, 1388, and 2873 cm⁻¹ assigned to nucleic acids, fatty acids and lipids, respectively showed highest discriminative power in distinguishing pathological from control tissues. In addition, the use of synchrotron radiation FTIR microspectroscopy enabled precise analysis of endomysium-specific changes. This work demonstrates the potential of microFTIR as a novel diagnostic tool for myopathy, offering an early-stage insight into muscle degradation that can support histopathological diagnosis. The ability of microFTIR to detect subtle biomolecular changes represents a promising step forward in non-invasive diagnostics of muscle disorders.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"344 ","pages":"Article 126727"},"PeriodicalIF":4.3,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144704002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperspectral imaging for trace cadmium prediction in lettuce leaves","authors":"Jun Sun ,&nbsp;Lei Shi ,&nbsp;Jiehong Cheng ,&nbsp;Chunxia Dai ,&nbsp;Xiaohong Wu","doi":"10.1016/j.saa.2025.126735","DOIUrl":"10.1016/j.saa.2025.126735","url":null,"abstract":"<div><div>Cadmium (Cd) pollution presents a significant threat to the agricultural product control, and the development of detection technology for Cd content in lettuce has important application value. This study developed a nondestructive approach based on hyperspectral imaging (HSI) to detect trace Cd content in lettuce. Using heterogeneous two-dimensional correlation spectroscopy (H2D-COS) to correlate visible near-infrared and fluorescence spectra, and introducing Savitzky Golay (SG) derivative method to preprocess spectral data, the weak features related to Cd were effectively amplified through noise suppression and signal enhancement, and the optimal band (407, 432, 453, 490, 543, 576, 626, 661, 688, 754, 779, 804, 826, 871, 891, 911, 935 and 964 nm) for Cd content was determined. The predictive performance of multiple feature extraction methods (including SPA, RC, RF, 2D-COS) combined with machine learning models were also compared. The results showed that H2D-COS significantly improved the ability to extract Cd-related features by fusing the dynamic response of heterologous spectra, and the constructed support vector machine (SVM) model exhibited the best performance. The research results indicate that H2D-COS integrated with SG derivative can extract the key bands, and combined with HSI, it has potential for prediction of trace Cd content in lettuce leaves.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"344 ","pages":"Article 126735"},"PeriodicalIF":4.3,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144704001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive detection of cervical cancer biomarker miR-21 using surface-enhanced Raman scattering (SERS)","authors":"Biqing Chen ,&nbsp;Xinyu Yao ,&nbsp;Shuwen Zhang ,&nbsp;Xinyue Hu ,&nbsp;Jiayin Gao ,&nbsp;Haizhu Sun ,&nbsp;Zhi Chen ,&nbsp;Xiaodong Sun ,&nbsp;Xiaohong Qiu ,&nbsp;Yang Li","doi":"10.1016/j.saa.2025.126725","DOIUrl":"10.1016/j.saa.2025.126725","url":null,"abstract":"<div><div>Cervical cancer's frequent late-stage diagnosis due to asymptomatic progression necessitates advanced early detection tools. This study pioneers a SERS platform for ultrasensitive detection of miR-21—a key cervical cancer biomarker—combining thiol-modified DNA probes for high-affinity miR-21 capture with AgNP-enhanced SERS substrates that convert hybridization events into amplified Raman signals. The platform achieves sensitivity in serum and demonstrates exceptional stability through DNA-RNA duplex formation, enabling robust reproducibility in complex biofluids. Unique spectral fingerprints enable precise target quantification while exhibiting promising potential for non-invasive lesion severity prediction. By overcoming critical limitations of conventional methods—including Pap smear's low sensitivity and HPV testing's inability to detect active cancer—this approach offers a rapid, specific liquid biopsy solution to enhance early diagnosis, dynamic monitoring, and clinical outcomes in cervical cancer management.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"344 ","pages":"Article 126725"},"PeriodicalIF":4.3,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dipyrromethene-based colorimetric and fluorescent sensors for the monitoring Zn2+ ions in aqueous media and food additives","authors":"Natalia A. Bumagina,&nbsp;Alexander A. Ksenofontov,&nbsp;Elena V. Antina,&nbsp;Mikhail B. Berezin","doi":"10.1016/j.saa.2025.126732","DOIUrl":"10.1016/j.saa.2025.126732","url":null,"abstract":"<div><div>Using 3,3′,5,5′-tetramethyl-2,2′-dipyrromethene (<strong>1</strong>), its <em>meso</em>-phenyl derivative (<strong>2</strong>) and 3,3′,5,5′-tetraphenyl-2,2′-dipyrromethene (<strong>3</strong>), the influence of the arylation effects of the <em>meso</em>-spacer and pyrrole cores of dipyrromethene bidentate chelate ligands (HL) on their colorimetric and fluorescent sensory characteristics towards Zn²⁺ ions in solutions was studied. The action of the sensors was based on the coordination reaction of dipyrromethene ligands by Zn²⁺ ions with the formation of stable (lg<em>K</em>^o = 5.2–8.9) intramolecular chelate complexes [ZnL₂]. It was found that the spectral responses of the reactions of HL with Zn(AcO)₂ in DMF/H₂O (9:1, <em>v</em>/v) differ greatly depending on the peripheral substitution nature of the dipyrromethene core. The analytical reaction of 3,3′,5,5′-tetramethyl-2,2′-dipyrromethene <strong>1</strong> with Zn(AcO)₂ is accompanied by bright color responses and high sensitivity of chromophore (LOD_abs = 1.7 × 10⁻⁸ M) and fluorescent (LOD_fl = 4 × 10⁻⁹ M) detection of the analyte. Arylation of the <em>meso</em>-spacer of 3,3′,5,5′-tetramethyl-2,2′-dipyrromethene leads to almost complete suppression of the fluorescence response (fluorescence enhancement factor ∼ 4) of reaction of <strong>2</strong> with the analyte. Sensitivity of chromophore-ratiometric detection of Zn²⁺ ions: LOD_abs is 1 × 10⁻⁸ M. Arylation of the pyrrole rings of ligand <strong>3</strong> leads to a significant decrease in the sensitivity of both spectrophotometric and fluorescent detection of the analyte. For the first time, encouraging results of practical application of dipyrromethene sensor <strong>1</strong> in the analysis of Zn²⁺ ions in samples of distilled and atmospheric water from melted snow, as well as extracts from food additives, were demonstrated.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"344 ","pages":"Article 126732"},"PeriodicalIF":4.3,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144704821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, crystal structure and optical properties of 2,4-diaminotoluene based Schiff Base: experimental and theoretical approaches","authors":"Keya Ghosh ,&nbsp;Ashok Mandi ,&nbsp;Nanda Gopal Bar ,&nbsp;Arindam Ray ,&nbsp;Gourab Kanti Das ,&nbsp;Dhrubajyoti Mondal ,&nbsp;Pranesh Chowdhury","doi":"10.1016/j.saa.2025.126731","DOIUrl":"10.1016/j.saa.2025.126731","url":null,"abstract":"<div><div>A Schiff base crystal [2,2′-((1E,1′E)-((4-methyl-1,3-phenylene) bis(azanylylidene))bis(methanylylidene))diphenol] based on 2,4-diaminotoluene has been synthesized and characterized by various analytical and spectroscopic methods. The synthesized Schiff base has been used as an excellent fluorescent sensor for Al³⁺ ions at the nanomolar level (5.68 × 10⁻⁹ M). The selective and potent metal-ligand interaction between Al³⁺ ions and the synthesized non-fluorescent Schiff base leads to a 1:2 adduct, which induces fluorescence-on (fluorescence quantum yield 22.4 %) through the chelation-enhanced fluorescence (CHEF) mechanism. Job's plot, ESI-MS, fluorescence titration, FTIR, ¹H NMR titration, and XPS analysis confirm the complex formation. The metal-ligand interaction has been interpreted theoretically by density functional theory (DFT) using Gaussian 09 software. The fluorescence-off stage of the metal-Schiff base adduct occurs through quenching by EDTA (both static and dynamic). A molecular logic gate has been constructed based on the observed off-on-off photoluminescence property of the Schiff base sensor in the presence of Al³⁺ ion and EDTA chelating ligand.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"344 ","pages":"Article 126731"},"PeriodicalIF":4.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel pyrido[2,3-d]pyrimidines for bioimaging: Effect of water content on enhanced fluorescence and the mechanism of aggregation-induced blue-shifted emission","authors":"Patrycja Rawicka ,&nbsp;Mateusz Korzec ,&nbsp;Mateusz Dulski ,&nbsp;Jacek Mularski ,&nbsp;Patryk Rurka ,&nbsp;Maria Książek ,&nbsp;Anna Mrozek-Wilczkiewicz ,&nbsp;Katarzyna Malarz","doi":"10.1016/j.saa.2025.126729","DOIUrl":"10.1016/j.saa.2025.126729","url":null,"abstract":"<div><div>Compounds containing the pyridopyrimidine core have attracted significant interest due to their promising biological properties and potential for medical applications. However, they represent an unexplored area in cellular imaging. Herein, a new class of aggregation-induced emission (AIE) fluorophores based on the pyrido[2,3-<em>d</em>]pyrimidine scaffold has been developed. We synthesized a small series of derivatives, which were evaluated for their spectroscopic properties in various solvents, considering fluorescence quantum yields, fluorescence lifetimes, and their decay components. Importantly, the physicochemical parameters were also evaluated in a MeOH/H₂O environment, suggesting the possible behavior of the derivatives in the cellular environment. Our analyses revealed the excellent photophysical properties of the ET06 compound, which exhibited enhanced fluorescence as a result of increasing water content, retaining significant Stokes shifts. An in-depth characterization of the molecular mechanism of fluorescence formation was linked to an aggregation-induced blue-shifted emission effect (AIBSE). TD-DFT calculations showed the existence of different spatial conformational states of ET06 in the single molecule state and aggregated states in at sandwich arrangement. Molecular conformational and energy changes in the ground and excited states, which had a significant effect on fluorescence emission in water-containing media, were also discussed. Finally, biological studies revealed negligible cytotoxicity of ET06 against cancer and normal cells. High fluorescence intensity in cells and a possible affinity and tendency to accumulate in mitochondria and lysosomes were also confirmed. These findings provide valuable insights into the molecular behavior and properties of pyrido[2,3-<em>d</em>]pyrimidines, boosting the landscape for designing new fluorescent probes for bioimaging.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"344 ","pages":"Article 126729"},"PeriodicalIF":4.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights on the synergistic effects of glycyrrhetinic acid and isoliquiritigenin on HMGB1-induced inflammation by multi-spectroscopic and molecular docking studies","authors":"Xuewa Jiang ,&nbsp;Shaoyan Yi ,&nbsp;Pingping Shen ,&nbsp;Richa Raj ,&nbsp;Jian Zhang ,&nbsp;Haixia Ge","doi":"10.1016/j.saa.2025.126726","DOIUrl":"10.1016/j.saa.2025.126726","url":null,"abstract":"<div><div>Chronic low-grade inflammation, driven by elevated levels of circulating high mobility group box1 (HMGB1), plays a crucial role in the pathogenesis of multiple chronic diseases. Glycyrrhetinic acid (GA) and isoliquiritigenin (ISL) are representative metabolites in licorice, exhibit potent regulatory effects on HMGB1-mediated chronic inflammation. In this research, we investigated the interactions of GA and ISL with HMGB1 using multi-spectroscopy, surface plasmon resonance (SPR), and molecular docking. Fluorescence spectroscopy revealed that GA or ISL binds to HMGB1, leading to static fluorescence quenching of the protein. Additionally, the binding of GA or ISL altered the microenvironment of tryptophan and decreased the α-helix content of HMGB1 to varying extents. Dynamic light scattering (DLS) showed that GA induced aggregation of the HMGB1 complex into large particles, while ISL reduced the particle size of HMGB1. SPR analysis confirmed that GA and ISL bound reversibly to HMGB1 with K_d values of 53.0 ± 3.6 and 16.3 ± 0.5 μM, respectively. Molecular docking further elucidated the binding modes and sites of GA and ISL on HMGB1, showing that GA binds to the B-box and ISL binds to the A-box of HMGB1, with hydrogen bonding and hydrophobic interactions as the primary driving forces. Isobologram analysis demonstrated that the combination of GA and ISL exhibited a significant synergistic inhibitory effect on HMGB1-induced inflammation on RAW264.7 cells, with an optimal combination ratio of 1:1. This study reveals that GA and ISL synergistically exert anti-inflammatory effect by modulating HMGB1 conformation, offering new insights into licorice extracts for preventing chronic inflammatory diseases.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"344 ","pages":"Article 126726"},"PeriodicalIF":4.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144703998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A near-infrared AIE probe targeting COX-2 for imaging of Cancer cells","authors":"Jianxi Liu ,&nbsp;Yonglin Zhang ,&nbsp;Jiale Hu ,&nbsp;Shulin Chen ,&nbsp;Ying Xue ,&nbsp;Wanhui Liu ,&nbsp;Li Shen ,&nbsp;Aiping Wang ,&nbsp;Yanping Zhu ,&nbsp;Lixiao Xu","doi":"10.1016/j.saa.2025.126696","DOIUrl":"10.1016/j.saa.2025.126696","url":null,"abstract":"<div><div>High expression of cyclooxygenase-2 (COX-2) in the inflammatory tumor microenvironment is a critical target for early cancer diagnosis. We designed and synthesized a series of near-infrared (NIR) fluorescent probes based on the aggregation-induced emission (AIE) mechanism for targeted COX-2 imaging. Leveraging a D-π-A quinoline-malononitrile core, we developed probes <strong>YL-180</strong> (non-targeted control), <strong>YL-181</strong> (celecoxib conjugate), and <strong>YL-186</strong> (indomethacin conjugate). Notably, this work represents the first report combining this specific AIE core with established COX-2 targeting ligands, celecoxib and indomethacin, for this application. These probes exhibit characteristic AIE properties, with <strong>YL-181</strong> showing fluorescence enhancement up to approximately 9.7-fold from pure THF in aggregated state, effectively overcoming the aggregation-caused quenching (ACQ) issue. They also possess favorable optical features including NIR emission (&gt;650 nm) and large Stokes shifts (&gt;200 nm). Their aggregation behavior and nanoparticle formation were characterized by DLS and TEM. In vitro cellular imaging revealed that <strong>YL-181</strong> achieved superior tumor cell selectivity, demonstrating approximately 22-fold higher fluorescence intensity in MCF-7 cancer cells over normal HUVEC cells (around 4-fold for <strong>YL-186</strong> over normal HUVEC cells). A competitive assay confirmed <strong>YL-181</strong>'s specific COX-2 binding. Furthermore, <strong>YL-181</strong> sensitively reflected intracellular COX-2 levels, with fluorescence decreasing by approximately 97 % from untreated upon COX-2 inhibition and increasing by around 135 % from untreated upon induction. Molecular docking and dynamics simulations provided insights into the specific binding mode and dynamic stability of <strong>YL-181</strong> with COX-2 from an atomic perspective. In vivo imaging validated <strong>YL-181</strong>'s excellent tumor targeting ability and high contrast performance in mouse models, showing a tumor-to-background ratio (TBR) of around 1.83 from normal tissue background, consistent with ex vivo organ analysis. Our highly sensitive and selective COX-2 targeted AIE probe, <strong>YL-181</strong>, holds significant potential for precise early tumor imaging.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"344 ","pages":"Article 126696"},"PeriodicalIF":4.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive detection of cyanazine residues using N, P-co-doped carbon dots/gold nanoparticles (N, P-CDs/AuNPs) fluorescent probe","authors":"Wenbo Li ,&nbsp;Maoxia Ran ,&nbsp;Haiyan Qi ,&nbsp;Tao Jing ,&nbsp;Jun Li","doi":"10.1016/j.saa.2025.126709","DOIUrl":"10.1016/j.saa.2025.126709","url":null,"abstract":"<div><div>A novel fluorescent probe based on nitrogen and phosphorus co-doped carbon dots (N, P-CDs) and gold nanoparticles (AuNPs) was developed for the sensitive and selective detection of cyanazine pesticide residues. N, P-CDs exhibited dual excitation wavelengths (460 nm and 490 nm) and a single emission peak at 515 nm. Upon conjugation with AuNPs, the prepared N, P-CDs/AuNPs fluorescent probe demonstrated fluorescence quenching via inner filter effect (IFE) and a remarkable restoration of fluorescence in the presence of cyanazine, attributed to the interaction between cyanide groups and gold nanoparticles. The probe exhibited high sensitivity with a detection limit of 0.14 μmol/L for cyanazine. Selectivity studies showed that the probe was minimally affected by common interfering substances, and recovery experiments in food samples demonstrated good accuracy with spiked recoveries ranging from 93.91 % to 114.30 %. This study provides a promising approach for detecting cyanazine in food samples with high specificity and reliability.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"344 ","pages":"Article 126709"},"PeriodicalIF":4.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}