Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy最新文献

{"title":"Regulating aggregation and optical properties of rigid-flexible ligand via metal coordination for multifunctional applications","authors":"Jun Wang ,&nbsp;Wenjing Shi ,&nbsp;Ying Xiong,&nbsp;Jinsheng Zhang","doi":"10.1016/j.saa.2025.126383","DOIUrl":"10.1016/j.saa.2025.126383","url":null,"abstract":"<div><div>Non-covalent interactions typically drive molecular aggregation, but precisely controlling supramolecular interactions to regulate molecular stacking configurations and expand practical applications remains challenging. Herein, we design and synthesize a rigid-flexible ligand <strong>Tpy-Py</strong>, incorporating one terpyridine (<strong>Tpy</strong>) and two pyrenes (<strong>Py</strong>) moieties. Whose aggregation state is regulated by metal coordination, thereby significantly modulating its optical properties. <strong>Tpy-Py</strong> exists as monomers in solution and forms excimers in the solid state, with emission shifting from blue to green. Single-crystal analysis reveals that <strong>Tpy-Py</strong> molecules form H- and J-aggregates via π–π stacking in the crystal, while coordination with Zn²⁺ and Cu²⁺ further tunes the molecular packing, leading to red-shifted emission or quenching. Moreover, the photochromic properties of <strong>Tpy-Py</strong> are effectively utilized in fingerprint detection, anti-counterfeiting, and visual detection of heavy metal ions. This work provides a new approach for regulating aggregation via non-covalent interactions and highlights the potential of <strong>Tpy-Py</strong> in multifunctional applications.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126383"},"PeriodicalIF":4.3,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel BODIPY probe for efficient detection and Photoinactivation of E.coli","authors":"Jie Zhang,&nbsp;Shi-Jie Li,&nbsp;Ling-Ling Qu,&nbsp;Xi-Yu Yang,&nbsp;Hai-Bing Sha,&nbsp;Qiu-Yun Chen","doi":"10.1016/j.saa.2025.126385","DOIUrl":"10.1016/j.saa.2025.126385","url":null,"abstract":"<div><div>Bacterial metabolism is related to pathogenicity, which lead to numerous diseases and pose significant threats to human health. Therefore, the development of metabolism-responsive probes is essential for early detection and effective treatment. In this study, we designed a novel BODIPY-based fluorescent probe (NH₂-BIN) targeting Escherichia coli (<em>E. coli</em>), guided by its copper homeostasis mechanism. NH₂-BIN exhibits high selectivity toward Cu²⁺ ions, forming a non-emissive complex (NH₂-BIN-Cu). Remarkably, <em>E. coli</em> is capable of restoring the fluorescence of NH₂-BIN-Cu through copper-binding and redox activity. The probe demonstrates an “on–off–on” fluorescence response with detection limits of 15 nM for Cu²⁺ and 1.04 CFU/mL for <em>E. coli</em>. Furthermore, NH₂-BIN-Cu displays photo-induced antibacterial activity against <em>E. coli</em> under red LED irradiation. These results highlight NH₂-BIN as a metabolism-driven, <em>E. coli</em>-responsive “sense-and-treat” fluorescence probe, offering a promising approach for precise detection and red-light-activated antibacterial therapy.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126385"},"PeriodicalIF":4.3,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of mitophagy in live cells with indole derived near-infrared fluorogenic probes","authors":"Xuanyuan Wang ,&nbsp;Wen Chen ,&nbsp;Shuangling Liu ,&nbsp;Yihong Xu ,&nbsp;Zhimei Xiong ,&nbsp;Yingzi Li ,&nbsp;Leyuan Huang ,&nbsp;Lu Jiang ,&nbsp;Jingting Zhang ,&nbsp;Leying Sun ,&nbsp;Yuan Zhang ,&nbsp;Mengqin Liu","doi":"10.1016/j.saa.2025.126368","DOIUrl":"10.1016/j.saa.2025.126368","url":null,"abstract":"<div><div>Mitophagy is an indispensable cellular process that plays a crucial role in regulating mitochondrial quality control and cellular metabolism. Therefore, monitoring the changes in the mitochondrial and lysosomal microenvironment during the mitophagy process is extremely important. However, existing mitophagy probes only target changes in a single indicator (viscosity, pH value, or polarity) within the microenvironment, which may reduce the selectivity and accuracy of assessing mitophagy in complex biological settings. To address this, we have developed a dual-channel detection near-infrared (NIR) fluorescent probe (ADMI). In vitro analysis experiments have shown that ADMI not only responds to pH and activates the NIR fluorescence channel but also that the green fluorescence channel exhibits high sensitivity to changes in polarity. This dual-response mechanism probe enables dual fluorescent detection of pH and polarity, providing a highly promising tool for monitoring the microenvironment of mitophagy in living cells. Ultimately, we applied ADMI to real-time monitoring of mitophagy induced by starvation or rapamycin, during which the decrease in pH and polarity resulted in a red shift in wavelength and increased fluorescence. Additionally, ADMI was able to observe changes in mitochondria during ferroptosis. This probe may serve as a useful tool for imaging mitophagy in living cells.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126368"},"PeriodicalIF":4.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid, visual and autocatalytic quantifying Ag(I) and Fe(Ⅲ) by ratiometric fluorescence sensor of N, Si, S-GQDs/OPD","authors":"Rui Zhou,&nbsp;Miao Dong,&nbsp;Yiwei Wu","doi":"10.1016/j.saa.2025.126366","DOIUrl":"10.1016/j.saa.2025.126366","url":null,"abstract":"<div><div>N, Si, S co-doped graphene quantum dots (N, Si, S-GQDs) were synthesized from waste toner and glutathione (GSH) via a one-pot hydrothermal method. Combined with o-phenylenediamine (OPD), a rapid, visual, and autocatalytic ratiometric fluorescence sensor of N, Si, S-GQDs/OPD was fabricated for detecting Ag(I) and Fe(III) ions by producing 2,3-diaminophenazine (oxOPD) under pH 8, which emits yellow fluorescence at 560 nm while quenching the blue fluorescence of N, Si, S-GQDs at 440 nm due to an inner filter effect. With the increase of Ag(I)/Fe(III), the blue fluorescence of N, Si, S-GQDs at 440 nm was gradually weakened, along with the enhancement of yellow fluorescence at 560 nm. Hence, this color change from blue to yellow under UV light enables semi-quantitative visual detection. The sensor demonstrates high sensitivity with detection limits of 0.016 µg mL⁻¹ for Ag(I) and 0.010 µg mL⁻¹ for Fe(III), and it successfully detects these ions in lake and tap water without pretreatment. The autocatalytic mechanism involves Ag(I) and Fe(III) reduction to Ag nanoparticles and Fe(II), respectively, which further catalyze the reaction, enhancing selectivity and efficiency. The method is cost-effective, simple, and suitable for on-site environmental monitoring.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126366"},"PeriodicalIF":4.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seeding multivariate algorithms for spectral analysis, a data augmentation approach to enhance analytical performance","authors":"M.E. Keating,&nbsp;H.J. Byrne","doi":"10.1016/j.saa.2025.126369","DOIUrl":"10.1016/j.saa.2025.126369","url":null,"abstract":"<div><div>Seeding spectral datasets by augmenting the data matrix with either the full spectrum or selected spectral features in order to bias multivariate analysis towards the solution of interest is explored. It is demonstrated that such seeding can have a profound effect on the endpoint of the analysis. Using Raman spectroscopic data of human lung adenocarcinoma cells (A549) in vitro, systematic perturbations to the spectra are introduced to simulate dose dependent exposure to a drug (cisplatin), and/or cellular response, representing reduced viability. Taking Principal Components Analysis (PCA) as the first example, seeding with the known spectral profiles of the drug exposure is demonstrated to greatly increase the ability of the algorithm to differentiate two distinct data subsets, representing control and exposed. The improved differentiation is quantified by further Linear Discriminant Analysis of the PCA data. Other examples of where seeding may be applied include, simulated datasets consisting of simultaneous changes in the spectral markers of exposure dose and cellular response, which are used for Multivariate Curve Resolution – Alternating Least Squares analysis (MCR-ALS). In the example presented, adding pure components to the dataset improves the ability of the algorithm to both model the systematic variation of concentration dependent data and extract the component spectra more accurately than the unseeded dataset. The seeded approach thus provides improved performance for differential analysis of datasets, as well as spectral unmixing analyses, to monitor, for example, the kinetic evolution of a reaction mixture, or metabolic pathway.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126369"},"PeriodicalIF":4.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient visible-light-active ZnO/Cs0.33WO3/g-C3N4 double Z-type heterojunction photocatalyst for rhodamine B photodegradation","authors":"Zainab H. Al Naji ,&nbsp;Yassine Slimani ,&nbsp;Munirah A. Almessiere ,&nbsp;Mohammed A. Gondal ,&nbsp;Atul Thakur ,&nbsp;Abdulhadi Baykal ,&nbsp;Anwar Ul-Hamid","doi":"10.1016/j.saa.2025.126380","DOIUrl":"10.1016/j.saa.2025.126380","url":null,"abstract":"<div><div>Developing highly efficient photocatalyst systems for the removal of cancer-causing organic dye substances from polluted water and wastewater is now in high demand because of the growing problem of contaminated water. An affordable technique was employed to create a ternary ZnO/Cs_0.33WO₃/g-C₃N₄ heterojunction nanocomposite with highly efficient and rapid photodegradation capabilities for degrading rhodamine B (RhB) dye. Using visible-light irradiation, the photocatalytic tests revealed that the proposed ZnO/Cs_0.33WO₃/g-C₃N₄ heterojunction nanocomposite is efficiently able to degrade more than 91 % of RhB dye within 15 min, 97.5 % within 20 min, and 99.9 % within 30 min, which is significantly efficient compared to sole ZnO and Cs_0.33WO₃. The kinetic rate constant of RhB photodegradation catalyzed by the ternary ZnO/Cs_0.33WO₃/g-C₃N₄ nanocomposite is assessed to be about 31 times faster than that of Cs_0.33WO₃ and about 3.5 times faster than that of ZnO. The investigation of the photodegradation mechanism suggested that the ternary ZnO/Cs_0.33WO₃/g-C₃N₄ nanocomposite follows a direct Z-scheme mechanism for charge transfer. The creation of a ternary ZnO/Cs_0.33WO₃/g-C₃N₄ heterojunction nanocomposite is valuable for increasing the surface area, strengthening the contact between its components, enhancing absorption capacity of visible light, increasing the generation rate of the photoexcited charge carriers, improving the separation efficiency of photogenerated charge carriers, and reducing their undesired recombination rate. As a consequence, the visible-light-mediated degradation of organic dye contaminants is significantly improved.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"341 ","pages":"Article 126380"},"PeriodicalIF":4.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Au@CuS@CuO2 nanoplatform with peroxidase mimetic activity and self-supply H2O2 properties for SERS detection of GSH","authors":"Xiang-Cheng Lin ,&nbsp;Jian Liu ,&nbsp;Miaomiao Hu ,&nbsp;Lingjun Song ,&nbsp;Minzhe Li ,&nbsp;Qinjie Kou ,&nbsp;Rong Huang ,&nbsp;Lixian Sun ,&nbsp;Changchun Wen","doi":"10.1016/j.saa.2025.126376","DOIUrl":"10.1016/j.saa.2025.126376","url":null,"abstract":"<div><div>The abnormal fluctuations of glutathione (GSH) in vivo often reflect disease progression and are closely linked to human metabolism and physiological functions. Highly sensitive and selective detection of GSH is crucial for guiding early diagnosis and treatment; however, achieving this remains a significant challenge. In this study, we developed an enzyme activity sensor platform for the efficient detection of GSH. This platform utilizes Au@CuS core–shell materials loaded with CuO₂ nanoparticles to create a composite nanosensor system. Under slightly acidic conditions, CuO₂ on the nanomaterial’s surface decomposes into H₂O₂ and Cu²⁺ ions. The generated H₂O₂ then reacts with tetramethylbenzidine (TMB) in the presence of peroxidase-like CuS to yield oxidized tetramethylbiphenyl (_OXTMB), which generates a distinctive Raman signal. Upon addition of GSH to the system, the unique _OXTMB signal diminishes due to GSH’s strong antioxidant capacity and the consequent consumption of _OXTMB. This sensing method enables sensitive detection of GSH, with a detection limit as low as 1.2 × 10^-13 mol∙L^-1. This approach holds promise for providing researchers with rapid and precise in vitro analysis of GSH, serving as an indicator for early disease diagnosis and real-time evaluation of treatment efficacy.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126376"},"PeriodicalIF":4.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assembly fluorescent probe-based detection of gamma-glutamyl transferase activity in tumor","authors":"Weiwang Zheng ,&nbsp;Jing Wu ,&nbsp;Nannan Song ,&nbsp;Baonan Zhang ,&nbsp;Chunhui Jin ,&nbsp;Weibo Zhao ,&nbsp;Kai Wang ,&nbsp;Shenghua Wang ,&nbsp;Xiaodan Zhu ,&nbsp;Cui Sun","doi":"10.1016/j.saa.2025.126381","DOIUrl":"10.1016/j.saa.2025.126381","url":null,"abstract":"<div><div>Detection of γ-glutamyltransferase (GGT) activity in cancer cells is crucial for understanding tumor progression and metabolic dysregulation. However, the accurate detection of GGT remains challenging due to its complex intracellular distribution and interactions with other enzymes. This study introduces a novel hemicyanine-based sensor, named GP, designed for the sensitive and real-time detection of GGT in tumor cells. By leveraging the unique optical properties of hemicyanines in the near-infrared spectrum, the GP sensor enables deeper tissue penetration and minimizes background interference, which is essential for tumor imaging. The sensor incorporates a GGT-specific recognition sequence that selectively binds to GGT, facilitating precise monitoring of its enzymatic activity through fluorescence signals generated by intramolecular charge transfer (ICT). Our results show that the GP probe exhibits high selectivity for GGT, distinguishing it from other biological molecules. Evaluation under tumor-relevant conditions confirms the probe’s robustness for both research and clinical applications. In cancer cell models, GP successfully detects increased GGT activity, suggesting its potential for non-invasive monitoring of tumor dynamics. Overall, this study demonstrates GP as a promising tool for the accurate detection of GGT activity, with significant implications for understanding cancer-related metabolic changes and tumor biology.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126381"},"PeriodicalIF":4.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondria-targeting probes with large Stokes shift for detecting Amyloid-β and cellular viscosity changes","authors":"Yingmei Cao,&nbsp;Jing Li,&nbsp;Jinwu Yan","doi":"10.1016/j.saa.2025.126378","DOIUrl":"10.1016/j.saa.2025.126378","url":null,"abstract":"<div><div>For effective in vivo applications, imaging probes must exhibit sufficient tissue penetration depth, high sensitivity, and specificity. Increasing evidence suggests that pathological accumulation of Aβ results in elevated mitochondrial viscosity. To achieve red-shifted absorption and emission characteristics of small-molecule theranostic agents and to enhance their mitochondrial targeting efficiency, a series of <strong>M</strong>−series probes (<strong>M13</strong> ∼ <strong>M15</strong>) was rationally designed based on the previously reported <strong>Q</strong>-series compounds. Using compound <strong>Q16</strong> as the parent structure, the <strong>M</strong> series probes retained the electron-donating dimethylamino group while replacing the benzene ring with a quinoline moiety. This modification was intended to enhance the intramolecular charge transfer (ICT) effect of the “D-π-A” system, thereby red-shifting the fluorescence emission wavelength and expanding the Stokes shift. The enhanced push–pull effect induced a redshift in the emission wavelength of probe <strong>M13</strong> to 806 nm in DMSO, resulting in a Stokes shift of 266 nm. This large Stokes shift effectively minimizes the overlap between excitation and emission wavelengths, thereby reducing self-quenching effects. Building on this, the interactions between <strong>M</strong>−series probes and Aβ aggregates were further explored. The probes exhibited the expected fluorescence characteristics and displayed varying degrees of response upon binding with Aβ aggregates. To enable a more precise early diagnosis, <strong>M13</strong>, <strong>M14</strong>, and <strong>M15</strong> were evaluated for their ability to monitor changes in mitochondrial viscosity and their mitochondrial targeting efficiency. The results demonstrated that the <strong>M</strong>−series fluorescent probes could effectively monitor variations in mitochondrial viscosity in cells. All three probes demonstrated strong mitochondrial targeting in HeLa cells, with <strong>M14</strong> achieving a high colocalization coefficient of 0.89 when compared with a commercial mitochondrial dye. These findings highlight the potential application of <strong>M</strong>−series probes in the early diagnosis and treatment of Alzheimer’s disease (AD).</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126378"},"PeriodicalIF":4.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecularly-imprinted pumice as a selective solid-phase extraction adsorbent for kojic acid determination in cosmetic samples","authors":"Adem Zengin ,&nbsp;Necdet Karakoyun ,&nbsp;Derya Çay Demir ,&nbsp;Mustafa Bilici","doi":"10.1016/j.saa.2025.126367","DOIUrl":"10.1016/j.saa.2025.126367","url":null,"abstract":"<div><div>Herein, a highly sensitive molecularly-imprinted pumice (MIP-PMC) was tailored by employed the surface imprinting method and utilized as a solid-phase extraction (SPE) adsorbent for the fast and selective separation of kojic acid (KA) from cosmetic samples. The MIP-PMC was characterized in a systematic manner and found it to be a well-grown polymer layer on the PMC. The adsorption capacity of MIP-PMC was 89.9 mg/g, with an equilibrium adsorption time of 30 min. Moreover, the imprinting factor of MIP-PMC towards KA was found to be 3.28, which further demonstrated its evident selectivity for KA. Under optimized conditions, KA was successfully isolated from cosmetic products using MIP-PMC and quantified via ultraviolet–visible (UV–vis) spectrophotometry. Method validation demonstrated excellent linearity within the range of 0.04–400 mg/L, satisfactory precision (less than 5 %), and high recoveries (98.0–100.4 %). Furthermore, the method exhibited a low limit of detection (LOD) of 12.8 μg/L with a preconcentration factor of 4. Moreover, the sensitivity of the developed method was also checked by using HPLC and the method has comparable analytical performance. The findings indicate that the integration of MIP-PMC based SPE with UV–vis spectrophotometry offers a highly efficient approach for sample pretreatment and the accurate determination of KA in real samples.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126367"},"PeriodicalIF":4.3,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}