Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy最新文献

{"title":"Peroxynitrite-responsive dicyanoisophorone-xanthene NIR fluorescent probe for monitoring and therapeutic evaluation of diabetic liver injury","authors":"Fei Kong ,&nbsp;Ming Yang ,&nbsp;Tao Yang ,&nbsp;Jingcan Qin","doi":"10.1016/j.saa.2025.127003","DOIUrl":"10.1016/j.saa.2025.127003","url":null,"abstract":"<div><div>Chronic hyperglycemia in diabetes induced liver injury (DLI) may progress to irreversible outcomes without timely intervention. It is highly beneficial to the development of a simple and rapid method for the early and accurate diagnosis of DLI. In this study, we developed an activatable near-infrared (NIR) fluorescent probe (DCIP-ONOO) based on a dicyanoisophorone-xanthene scaffold, for monitoring and therapeutic evaluation of DLI <em>via</em> Peroxynitrite (ONOO⁻) imaging. Upon the addition of ONOO⁻, DCIP-ONOO exhibited a 27.6-fold fluorescence enhancement at 770 nm with an excellent selectivity, fast responsiveness, and a low limit of detection (119.6 nmol/L). <em>In vitro</em> imaging experiments confirmed that it was capable of being employed for the sensitive detection of ONOO⁻. More significantly, in a diabetic mice model, DCIP-ONOO was successfully used to achieve <em>in vivo</em> imaging of DLI and to monitor metformin-mediated symptom alleviation without causing any organ damages. These results established DCIP-ONOO as a potential tool for effective tracking of ONOO⁻ during diabetic hepatotoxicity.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 127003"},"PeriodicalIF":4.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A molecular imprinted fluorescence sensor based on EDTA-Cu quantum dots for methamphetamine recognition","authors":"Yanqin Zhu ,&nbsp;Le Wang ,&nbsp;Jinwen Zhao ,&nbsp;Jiazhong Zhang ,&nbsp;Qinhong Yin","doi":"10.1016/j.saa.2025.127001","DOIUrl":"10.1016/j.saa.2025.127001","url":null,"abstract":"<div><div>Methamphetamine (METH) is a potent psychostimulant with a high potential for addiction, ranking among the most prevalent and frequently abused illicit substances worldwide. Thus, the development of rapid and precise methodologies for METH detection is essential for law enforcement and public health monitoring. This study reported the first construction of a molecularly imprinted fluorescence sensor utilizing EDTA-Cu quantum dots (EDTA-Cu QDs-MIP) for the fluorescence quantification of methamphetamine. The sensor displayed a linear response within a concentration range spanning from 53 to 371 nM, achieving a limit of detection (LOD) equal to 20.6 nM through fluorescence spectroscopy. Validation with real-world samples confirmed the sensor's high accuracy, with recovery rates in the range from 97.2 to 104.4 %. This study not only broadens the scope of QDs in chemical detection but also introduces a strong and portable platform for forensic investigations and on-site screening of illicit drugs.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 127001"},"PeriodicalIF":4.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ratiometric fluorescence and Nanozyme colorimetry for dual-modality uric acid detection in complex Biosystems","authors":"Wei Li ,&nbsp;Yongtao Xu ,&nbsp;Wuyuan Pan ,&nbsp;Shuqing Tu ,&nbsp;Jian Wang ,&nbsp;Fang Ke","doi":"10.1016/j.saa.2025.127008","DOIUrl":"10.1016/j.saa.2025.127008","url":null,"abstract":"<div><div>Humans lack urate oxidase (UOX) due to UOX gene mutations, leading to hyperuricemia-related disorders like gout and kidney disease. Accurate uric acid (UA) quantification is therefore vital for diagnosis and management. Conventional methods are often impractical, while popular enzymatic colorimetric assays, though simpler, suffer from cross-reactivity and matrix interference. Nanozymes, such as graphitic carbon nitride (g-C₃N₄), offer robust, cost-effective alternatives; Mn-doping C₃N₄ (Mn-C₃N₄) enhances its uricase-like activity and stability. However, real-world application of Mn-C₃N₄ is still challenged by selectivity issues and matrix effects. To overcome these limitations, we developed a synergistic dual-detection platform integrating Mn-C₃N₄ nanozymes applied together with the ratiometric fluorescent probe MPNVPI. Under blue-light irradiation (460 nm), Mn-C₃N₄ oxidizing UA to generate H₂O₂ triggers an event: nucleophilic cleavage of MPNVPI releases fluorescent HVMI (emission shift: 500 nm to 568 nm). This label-free, dual-modality assay leverages intrinsic optical properties for ultra-sensitive UA quantification in complex serum matrices. It also enables real-time imaging of UA dynamics in RAW 264.7 cells and zebrafish, demonstrating exceptional sensitivity, selectivity, and monitoring capability for diagnostic applications in metabolic disorders.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 127008"},"PeriodicalIF":4.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multifunctional fluorescent probe for detecting metal cations based on a novel zinc coordination polymer","authors":"Wei Zhao ,&nbsp;Ai Wang ,&nbsp;Sisi Feng ,&nbsp;Caixia Yuan ,&nbsp;Ulli Englert","doi":"10.1016/j.saa.2025.127004","DOIUrl":"10.1016/j.saa.2025.127004","url":null,"abstract":"<div><div>A novel zinc-based coordination polymer, namely [Zn(HDPNA)(2,2′-bipy)]_n (<strong>1</strong>, H₃DPNA = 2-(3,5-dicarboxylphenyl)nicotinic acid, 2,2′-bipy = 2,2′-bipyridine), was successfully constructed through a mixed-ligand strategy. Fluorescence analysis revealed that <strong>1</strong> displayed selective fluorescence enhancement exclusively towards Ag⁺ ions, with a detection limit of 9.73 × 10⁻⁹ M, which may be due to π···Ag⁺···π interactions formed between Ag⁺ ions and the suitably arranged ligands in the polymer. Molecular docking results confirmed that such interactions enhance electron delocalization and inhibit non-radiative transitions. In addition, <strong>1</strong> exhibited highly selective luminescence quenching responses towards Cu²⁺ and Co²⁺ ions, with detection limits of 8.75 × 10⁻⁹ M and 4.36 × 10⁻⁹ M, respectively. The addition of excessive glutathione enabled <strong>1</strong> to display different ‘on’ and ‘off’ behaviors towards Cu²⁺ and Co²⁺ and thus allowed to assess even mixtures of these cations. With respect to the detection mechanism of <strong>1</strong> towards Cu²⁺ and Co²⁺, we suggest based on theoretical calculations that photoinduced electron transfer (PET) may lead to fluorescence quenching. Detection experiments under realistic conditions have proved that this new fluorescence sensor can be applied to the detection of Ag⁺, Cu²⁺ and Co²⁺ in real life samples, e.g. tap water. Coordination polymer <strong>1</strong> represents an efficient and multifunctional chemical sensor platform based on ‘on-off’ fluorescence detection and may provide guidance for the advanced development of multi-ions sensors based on coordination compounds.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 127004"},"PeriodicalIF":4.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel fluorescent probe for in vivo H₂S monitoring in inflammatory arthritis","authors":"Yihan Wang,&nbsp;Yifanghui Gao,&nbsp;Jiaqi Zhou,&nbsp;Yuan Jiang,&nbsp;Yue Wang,&nbsp;Cheng Zhang,&nbsp;Qingtao Meng,&nbsp;Zhiqiang Zhang","doi":"10.1016/j.saa.2025.127007","DOIUrl":"10.1016/j.saa.2025.127007","url":null,"abstract":"<div><div>Hydrogen sulfide (H₂S), a crucial gaseous signaling molecule, plays a pivotal regulatory role in various physiological and pathological processes. In this study, we successfully developed a novel H₂S fluorescent probe <strong>NR</strong> based on the Nile red fluorophore. The probe achieves specific recognition of H₂S through an isothiocyanate functional group, and its recognition mechanism was confirmed by high-resolution mass spectrometry. Probe <strong>NR</strong> exhibits excellent stability and high sensitivity, with a detection limit of 3.66 μM. Demonstrating low cytotoxicity and suitability for physiological pH conditions, probe <strong>NR</strong> has been successfully applied for real-time monitoring of H₂S in vivo. In an LPS-induced arthritis model in nude mice, probe <strong>NR</strong> effectively enabled dynamic monitoring of H₂S levels at inflammatory sites. The successful development of this probe provides a powerful tool for investigating the pathophysiological mechanisms of H₂S in inflammatory diseases, demonstrating promising potential for disease diagnosis and therapeutic monitoring</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 127007"},"PeriodicalIF":4.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of near-infrared two-photon absorption response and excited state dynamics of asymmetric aza-BODIPY derivatives","authors":"Anıl Doğan ,&nbsp;Halil Yılmaz ,&nbsp;Elif Akhüseyin Yıldız ,&nbsp;Gökhan Sevinç ,&nbsp;Fikret Arı ,&nbsp;Huseyin Unver ,&nbsp;Bahadir Boyacioglu ,&nbsp;Mustafa Hayvali ,&nbsp;Ayhan Elmali ,&nbsp;Ahmet Karatay","doi":"10.1016/j.saa.2025.127010","DOIUrl":"10.1016/j.saa.2025.127010","url":null,"abstract":"<div><div>The study focused on asymmetric aza-BODIPY derivatives, which were modified with 4-bromophenyl (<strong>B1</strong>), 4-bromo and 4-bromophenyl (<strong>B2</strong>), 4-<em>N,N</em>-diphenylaminobiphenyl (<strong>B3</strong>), 4-methoxybiphenyl (<strong>B4</strong>), 4-methoxyphenyl and 4-bromophenyl (<strong>B5</strong>), and 2,4-dimethoxybiphenyl (<strong>B6</strong>) groups at 2,6 and β positions of aza-BODIPY skeleton. The compounds were thoroughly investigated to understand their linear optical properties, near-infrared nonlinear absorption responses, and dynamics of excited states. The linear absorption and fluorescence behaviors of the compounds were studied depending on the substituents and solvents (THF, CHCl₃). Compounds <strong>B1</strong> and <strong>B4</strong> showed higher absorption in THF, while compound <strong>B5</strong> exhibited higher absorption in CHCl₃. No significant change was observed in compounds <strong>B2</strong>, <strong>B3</strong>, and <strong>B6</strong>. The fluorescence spectra revealed that compounds <strong>B1</strong>, <strong>B4</strong>, and <strong>B6</strong> exhibit comparatively higher fluorescence quantum yields in CHCl₃ medium than in THF. The fluorescence of <strong>B2</strong>, <strong>B3</strong>, and <strong>B5</strong> was significantly quenched due to the strong intramolecular charge transfer (ICT) effects influenced by the electron-donating diphenylamine unit and methoxy groups, as well as the heavy atom effect of bromine atoms. Excited state dynamics of all compounds were revealed with pump-probe spectroscopy. Near-infrared two-photon absorption (TPA) response at 1000 nm was observed for all the studied compounds. Compound <strong>B3</strong> indicated the highest TPA cross-section (TPCS) (210 GM) due to high ICT capability of diphenylaminobiphenyl unit. Compounds <strong>B3</strong> and <strong>B6</strong> stand out with their strong photophysical properties; <strong>B3</strong> exhibited high ICT activity and the highest TPCS, while <strong>B6</strong> had high fluorescence quantum yield.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 127010"},"PeriodicalIF":4.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A flavonol-based fluorescent probe for N2H4 detection and its applications in natural environmental samples","authors":"Yu Wang,&nbsp;Licheng Li,&nbsp;Zhonglong Wang","doi":"10.1016/j.saa.2025.126982","DOIUrl":"10.1016/j.saa.2025.126982","url":null,"abstract":"<div><div>Hydrazine possesses a diverse range of applications and exhibits favorable water solubility, which can lead to contamination and toxicity in both the natural environment and human beings due to improper handling. Therefore, it is particularly important to establish a reliable and accurate analytical method for detecting hydrazine. In this study, we designed and synthesized a flavonoid-based fluorescent probe <strong>DP-N</strong>_{<strong>2</strong>}<strong>H</strong>_{<strong>4</strong>} that specifically tailored for hydrazine detection, thoroughly investigating its spectral properties and response mechanism. <strong>DP-N</strong>_{<strong>2</strong>}<strong>H</strong>_{<strong>4</strong>} demonstrated exceptional selectivity towards N₂H₄ with an impressively low detection limit of 0.468 μM, showcasing remarkable sensitivity towards N₂H₄ as well. Furthermore, our findings demonstrated that the probe effectively recognized hydrazine in various environmental and biological samples including ambient water, soil, and living cells.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126982"},"PeriodicalIF":4.6,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescence sensor based on molecularly imprinted polymer and bimetallic Tb/Ce MOF for sensitive detection of thiamethoxam","authors":"Xinzhuang Wei ,&nbsp;Yaming Zhao ,&nbsp;Huifeng Zhang ,&nbsp;Qiong Jia","doi":"10.1016/j.saa.2025.127005","DOIUrl":"10.1016/j.saa.2025.127005","url":null,"abstract":"<div><div>With the extensive use of neonicotinoid pesticides like thiamethoxam (TMX), there is a growing need to develop sensitive and selective methods to monitor its residue levels. In this study, we developed a fluorescence sensor based on terbium/cerium metal-organic framework combined with molecularly imprinted polymer (Tb/Ce MOF@MIP) for TMX detection. Through systematic optimization of synthesis conditions and detection conditions, the sensor was successfully employed for the determination of TMX, exhibiting high selectivity, along with a linear detection range of 1–50 μM and a limit of detection of 0.8 μM. In addition, the practical applicability of the sensor was validated in real samples including apples, tea, and soil, and the accuracy was assessed through recovery experiments.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 127005"},"PeriodicalIF":4.6,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145215048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Point-of-need detection of Pb2+ ions using a smartphone-enabled fluorescent paper sensor with modified calix[4]arene scaffold","authors":"Krushna A. Baraiya ,&nbsp;Heni N. Soni ,&nbsp;Sahaj A. Gandhi ,&nbsp;Saim A. Khatri ,&nbsp;Pinkeshkumar G. Sutariya","doi":"10.1016/j.saa.2025.126996","DOIUrl":"10.1016/j.saa.2025.126996","url":null,"abstract":"<div><div>In this study, we report the development of a novel fluorescence sensor based on calix[4]arene-crown[5] (C4CBy) functionalized with bansyl chloride as a fluorophore. The synthesized probe was characterized using various spectroscopic techniques, including MALDI-TOF-MS, FT-IR, ¹H NMR, ¹³C NMR, and 135 DEPT NMR. Emission spectroscopy analysis demonstrated that C4CBy exhibits high selectivity towards Pb²⁺ ions, leading to a significant enhancement in fluorescence intensity. This fluorescence enhancement is attributed to the ligand-to-metal charge transfer (LMCT) mechanism, with a linear response observed over a Pb²⁺ concentration range of 0–100 nM (R² = 0.9927) and a detection limit of 1.65 nM. A Job's plot analysis confirmed a 1:1 stoichiometric binding ratio between C4CBy and Pb²⁺ ions via fluorescence spectroscopy. To evaluate the practical applicability of C4CBy, the sensor was tested in real sample matrices, including drinking water, lake water, laboratory water, cow milk, buffalo milk, orange, lake soil, industrial soil, and farm soil samples, within a Pb²⁺ concentration range of 0–30 nM. Additionally, a disposable paper-based analytical device was developed, along with a smartphone-integrated portable sensing platform, providing a cost-effective, user-friendly, and highly selective approach for Pb²⁺ detection.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126996"},"PeriodicalIF":4.6,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ratiometric fluorescent probe with a large emission shift for the detection of sulfur dioxide derivatives in live cells, foods and plant","authors":"Junjie Chen ,&nbsp;Haijian Meng ,&nbsp;Yichao Wen ,&nbsp;Dongmei Deng ,&nbsp;Yonggang Min","doi":"10.1016/j.saa.2025.126999","DOIUrl":"10.1016/j.saa.2025.126999","url":null,"abstract":"<div><div>Sulfur dioxide (SO₂) derivatives fulfill essential roles within food additives, specific physiological contexts, and plant development mechanisms, yet heightened exposure levels are associated with adverse effects on human health and plant viability. Thus, the ratiometric fluorescent probe SX-TPA was engineered for detecting SO₂ derivatives, boasting high sensitivity (detection limit: 0.201 μM), excellent selectivity and anti-interference capability, and rapid response. SX-TPA discriminates SO₂ derivatives specifically via Michael nucleophilic addition. This reaction disrupts the π-conjugation within the probe skeleton and blocks the intramolecular charge transfer (ICT) process, thus eliciting a marked emission shift (Δλ = 259 nm). Importantly, the SX-TPA exhibits outstanding imaging and detection capabilities even in complex real food samples, biological living cells, and the epidermal tissue of plant onions.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126999"},"PeriodicalIF":4.6,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}