Chang Liu, Chuang Feng, Yan Liu, Yuanyuan Wu, Huirong Yao, Song He, Xianshun Zeng
{"title":"Construction of a novel NIR-emissive rhodamine derivative for monitoring mitochondrial viscosity in ferroptosis","authors":"Chang Liu, Chuang Feng, Yan Liu, Yuanyuan Wu, Huirong Yao, Song He, Xianshun Zeng","doi":"10.1016/j.saa.2024.125486","DOIUrl":"10.1016/j.saa.2024.125486","url":null,"abstract":"<div><div>Ferroptosis, an iron-dependent programmed cell death mechanism, is mediated by distinct molecular pathways of lipid peroxidation caused by intracellular iron supplementation and glutathione synthesis inhibition that cause oxidative damage to the cell membrane. Monitoring viscosity changes of mitochondria is essential for a deeper understanding of ferroptosis, as mitochondria will be shrunk with increased membrane density and leading to drastic mitochondrial viscous changes during ferroptosis process. Thus, it is essential to explore novel and efficient fluorescent probes for monitoring viscosity in organisms. In this work, we designed and synthesized a mitochondria-targeting probe <strong>TJ-FRP</strong> for cellular viscosity measurement <em>via</em> fluorescence imaging method. To obtain this probe, we firstly developed a novel modifiable fluorescent π-extended xanthene dye <strong>TJ-FR</strong> by replacing the benzoic acid group with a strong electron-withdrawing perfluorobenzoic acid group at the 9-position of xanthene framework. The dye not only presents emission wavelength at 758 nm and a large stokes shift of 142 nm in water, but also the dye is low biotoxic, membrane permeable. By reaction with 4-aminobutyltriphenylphosphonium bromide, <strong>TJ-FR</strong> was converted to the mitochondria-targeting probe <strong>TJ-FRP</strong>. <strong>TJ-FRP</strong> was successfully applied for the imaging of viscosity in living cells. Especially, the probe can be applied for visualizing mitochondrial viscosity changes during various inducers-stimulated ferroptosis process in model cells. These findings suggest that this novel NIR fluorescent probe can serve as a powerful tool to monitor the viscosity in biological samples and may provide new insights for various diseases during ferroptosis.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"328 ","pages":"Article 125486"},"PeriodicalIF":4.3,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hongru Liu, Yiting Yang, Yan Yan, Jie Yu, Shuo Chai
{"title":"Vibrational ladder climbing dissociation of LiH molecules excited by non-chirped pulses","authors":"Hongru Liu, Yiting Yang, Yan Yan, Jie Yu, Shuo Chai","doi":"10.1016/j.saa.2024.125460","DOIUrl":"10.1016/j.saa.2024.125460","url":null,"abstract":"<div><div>Vibrational ladder climbing (VLC) is one of the advanced methods for achieving molecular dissociation, usually requires the use of chirped laser pulses to adapt to the change of energy difference between molecular vibrational levels. In this work, a scheme is proposed for using non-chirped pulses to couple the vibrational rotational levels of excited state to realize VLC dissociation of LiH molecules in the excited state. The first pulse induces population excitation to the excited state <span><math><mrow><msup><mrow><mi>A</mi></mrow><mrow><mn>1</mn></mrow></msup><msup><mrow><mi>Σ</mi></mrow><mrow><mo>+</mo></mrow></msup></mrow></math></span>, while the second pulse is used to drive the excited state vibrational levels population up step by step until dissociation occurs. The non-chirped pulse VLC dissociation dynamics is investigated using the time-dependent quantum wave packet theory method. The “”climb steps” process for excited state molecules at low full width at half maximum (FWHM) of the second laser pulse is demonstrated. The frequency has an obvious influence on the distribution of excited state vibrational rotational levels and further affects the relaxation process of the undissociated part and the angular distribution of dissociation products. It is also explained that the anomalous decrease of dissociation probability is caused by vibrational levels oscillation.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"328 ","pages":"Article 125460"},"PeriodicalIF":4.3,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xi Kang , Shanting Liu , Yijie Zou , Zewei Song , Yong Jiang , Hui-juan Zhang , Hai-feng He , Shouzhi Pu , Zhao Chen
{"title":"Fluorene-containing binuclear gold(I) complexes: High-contrast mechanochromic fluorescence quenching and information encryption application","authors":"Xi Kang , Shanting Liu , Yijie Zou , Zewei Song , Yong Jiang , Hui-juan Zhang , Hai-feng He , Shouzhi Pu , Zhao Chen","doi":"10.1016/j.saa.2024.125489","DOIUrl":"10.1016/j.saa.2024.125489","url":null,"abstract":"<div><div>A series of fluorene-based binuclear gold(I) complexes <strong>I–VI</strong> have been successfully synthesized. Their structures were characterized by nuclear magnetic resonance, high-resolution mass spectrometry, and single crystal X-ray diffraction techniques. The fluorescence switching characteristics of complexes <strong>I–VI</strong> in the solid state were studied by photoluminescence spectroscopy. Luminogens <strong>I–VI</strong> showed different solid-state fluorescence involving blue-green, yellow-green and yellow colors before mechanical stimulation, which suggests that the solid fluorescent properties of <strong>I–VI</strong> can be effectively manipulated by positional isomerism and increasing conjugation strategies. Interestingly, their solid fluorescence intensities of all luminogens weakened significantly upon grinding. Impressively, luminogen <strong>IV</strong> barely displayed macroscopic fluorescence after grinding, indicative of its remarkable high-contrast mechanochromic fluorescence quenching feature. The unique mechanofluorochromic phenomena of <strong>I–VI</strong> were fully elucidated by analyzing X-ray diffraction patterns changes of <strong>I–VI</strong> before and after grinding and crystal packing structure of <strong>III</strong>. Furthermore, an effective dual information encryption system was constructed based on complex <strong>IV</strong>.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"328 ","pages":"Article 125489"},"PeriodicalIF":4.3,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaohu Mi , Xin Zhao , Yuting Luo , Yongfeng Li , Yuwei Wu , Simeng Xue , Xiaohang Yang , Zhenglong Zhang , Haifa Qiao
{"title":"Au nanoflower/Au island hybrid substrate as high-performance SERS platforms","authors":"Xiaohu Mi , Xin Zhao , Yuting Luo , Yongfeng Li , Yuwei Wu , Simeng Xue , Xiaohang Yang , Zhenglong Zhang , Haifa Qiao","doi":"10.1016/j.saa.2024.125483","DOIUrl":"10.1016/j.saa.2024.125483","url":null,"abstract":"<div><div>Surface-enhanced Raman scattering (SERS) offers significant enhancements to weak Raman signals, which has been widely used for the detection of ultra-low concentrations of molecules. Obtaining repeatable SERS substrates with high density “hot spots” is one of the main challenges for quantitative analysis using SERS. Herein, a SERS substrate based on Au nanoflower/nanoisland (NF/NI) hybrid substrate is constructed. The Au NF/NI substrate can generate “hot spots” by the nanogap between nanotip of Au NF and Au NI, which has been proved to have high stability and sensitivity by experiments, the limit of detection is determined to be down to 10<sup>−11</sup> M for Rhodamine 6G (R6G) molecules. In addition, the Au NF/NI hybrid substrate as template can be further extended to prepare bimetallic SERS substrates. This synthetic pathway will not only contribute to the design of new type of SERS substrate, but also promote understanding of the growth mechanisms of multicomponent nanostructure.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"328 ","pages":"Article 125483"},"PeriodicalIF":4.3,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rui Yang , Qinyi Gao , Xue Lu , Yukun Wu , Changxin Zhu , Zhida Han , Chuanya Li , Mengmeng Wei
{"title":"Wash-free and ultra-low concentration monitor lysosomal viscosity in apoptosis with a noteworthy fluorescent probe","authors":"Rui Yang , Qinyi Gao , Xue Lu , Yukun Wu , Changxin Zhu , Zhida Han , Chuanya Li , Mengmeng Wei","doi":"10.1016/j.saa.2024.125481","DOIUrl":"10.1016/j.saa.2024.125481","url":null,"abstract":"<div><div>The visualization and subsequent monitoring of apoptosis holds paramount significance in the domains of physiology, pathology, and pharmacology. However, traditional probes require high staining concentrations and multiple washing steps, which would alter the specimen’s micro-environment, potentially inducing harm to specimen. To overcome these challenging issues, we have rationally designed and prepared a pH-inert lysosomal probe (named <strong>IVTI</strong>) to wash-free visualize apoptosis with ultra-low concentration to alleviate the disturbance of probe concentration, washing procedure and pH variations. Compared with general lysosomal probes, <strong>IVTI</strong> showed a significant fluorescence boost in reflex to elevated viscosity, while its fluorescence intensity remained mostly still when altering pH values, which could achieve more accurate visualization of lysosomes. Moreover, the probe can detect minute viscosity fluctuations in lysosomes under extra-low concentration, greatly eliminating the effect of probe concentration and washing steps to live bio-samples. Furthermore, compared to LTR (Lyso-Tracker Red, a commercial lysosome probe), <strong>IVTI</strong> offered exceptional imaging capabilities, and the fluorescence images of <strong>IVTI</strong> was still clear when lysosomal pH increased, which attributed to the pH-inert properties of <strong>IVTI</strong>. In view of the excellent imaging abilities, the pH-inert probe was applied to in-situ and real time visualize viscosity changes of live cells under extra-low concentration without washing procedure, and the increase of lysosomal viscosity during apoptosis was also monitored by the probe, thereby minimizing the disturbance of probe concentration, washing procedure and pH variations during apoptosis. The probe possesses tremendous potential in the visualization of dynamic changes related to lysosomes in various physiological processes.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"329 ","pages":"Article 125481"},"PeriodicalIF":4.3,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Stable fluorimetric detection of histamine using o-phthaldialdehyde derivatizing agent and surfactants in water-acetonitrile mixed solvent","authors":"Merymene Boukadida , Amira Anene , Najeh Jaoued , Anne Baudouin , Yves Chevalier , Souhaira Hbaieb","doi":"10.1016/j.saa.2024.125488","DOIUrl":"10.1016/j.saa.2024.125488","url":null,"abstract":"<div><div>A new analytical method for the rapid detection of histamine (HA) in beverages using fluorescence detection has been devised. It is primarily based on the use of o-phthalaldehyde (OPA) as a derivatizing agent. This unstable complex has been efficiently stabilized by addition of surfactants. The physicochemical variables that influence the sensitivity of the method and the fluorescence properties of the complex species in surfactant solutions have been optimized. Different surfactants were checked: the anionic sodium dodecyl sulfate, the cationic hexadecyltrimethylammonium chloride and the nonionic tricosa(ethylene glycol)dodecyl ether C<sub>12</sub>E<sub>23</sub>. All surfactants stabilize the HA:OPA complex for long times. C<sub>12</sub>E<sub>23</sub> provides excellent stability to the complex and higher fluorescence emission, allowing robust analysis conditions. Linear calibration curves allowing effective histamine determination and low limits of detection and quantification were established and the characteristics of the analysis method was validated. The correlation coefficient for histamine detection by fluorescence was 0.9978. The detection and quantification limits for histamine were 0.576 and 1.92 mg∙L<sup>−1</sup>, respectively.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"329 ","pages":"Article 125488"},"PeriodicalIF":4.3,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sara M. Ragab , Mohamed M. Aboelnga , Elhossein A. Moawed , Rana R. Elsadda , Gasser M. Khairy
{"title":"Enhanced glutathione sensing in clinical samples with Bovine Serum Albumin-stabilized copper nanoclusters: A novel fluorescent probe","authors":"Sara M. Ragab , Mohamed M. Aboelnga , Elhossein A. Moawed , Rana R. Elsadda , Gasser M. Khairy","doi":"10.1016/j.saa.2024.125465","DOIUrl":"10.1016/j.saa.2024.125465","url":null,"abstract":"<div><div>Glutathione (GSH) is a critical biomolecule involved in maintaining protecting against both oxidative stress and cellular redox homeostasis, making its accurate detection essential for clinical diagnostics. This study introduces a novel, highly sensitive, and selective fluorescence-based method for detecting GSH in human serum samples, using copper nanoclusters stabilised with Bovine Serum Albumin (BSA-CuNCs) as a probe. The BSA-CuNCs display a distinct fluorescence emission peak at 402 nm when excited at 326 nm, demonstrating exceptional photostability and structural integrity. The interaction with GSH not only significantly enhances the fluorescence intensity but also induces a red shift in the emission peak, attributed to the chemisorption of GSH onto the BSA-CuNCs surface, as confirmed by IR, TEM, and EDS analyses. This method achieves a broad linear detection range spanning from 2.95 to 207 µM and a highly sensitive detection limit of 79 nM, outperforming many existing detection techniques with respect to both selectivity and sensitivity. The BSA-CuNCs probe has been effectively used to measure the amount of GSH in human serum, with recovery values between 95.07 % and 101.38 %. Furthermore, the experimental results have been confirmed by density functional theory (DFT) calculations and molecular docking, yielding a full comprehension of the interaction mechanisms at the molecular scale. The novelty of this approach lies in its combination of high selectivity, sensitivity, and biocompatibility, making it a valuable tool for the early monitoring and diagnosis of diseases related to oxidative stress, such as cancer and neurodegenerative disorders. The method’s simplicity, cost-effectiveness, and potential for adaptation to other biological targets underscore its significance.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"328 ","pages":"Article 125465"},"PeriodicalIF":4.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rumei Cheng , Zhangliang Li , Pingjun Chang , Suyan Shan , Xiaohui Jiang , Zhixuan Hu , Bei Zhang , Yune Zhao , Shengju Ou
{"title":"Enhanced intracellular calcium detection using dopamine-modified graphene quantum dots with dual emission mechanisms","authors":"Rumei Cheng , Zhangliang Li , Pingjun Chang , Suyan Shan , Xiaohui Jiang , Zhixuan Hu , Bei Zhang , Yune Zhao , Shengju Ou","doi":"10.1016/j.saa.2024.125475","DOIUrl":"10.1016/j.saa.2024.125475","url":null,"abstract":"<div><div>The tracking of calcium ions (Ca<sup>2+</sup>) is of great significance in clinical medicine. Dopamine-functionalized graphene quantum dots (DA-GQDs) have been developed as a novel fluorescence sensor for detecting and imaging Ca<sup>2+</sup> with high selectivity and sensitivity. Upon the addition of various concentrations of Ca<sup>2+</sup>, the fluorescence intensity of DA-GQDs notably enhanced and exhibited a redshift. This behavior was regulated by the photoinduced electron transfer and internal charge transfer mechanisms, as elucidated by fluorescence titration and density functional theory calculations. The coordination of Ca<sup>2+</sup> with the oxygen groups on DA-GQDs enhanced its sensitivity and selectivity. The detection limit was determined to be 0.05 µM, with a robust linear response range of 4.93–10.61 µM. Even in the presence of chelating agents such as gluconate, the DA-GQDs accurately quantified Ca<sup>2+</sup>. Competition experiments demonstrated that the DA-GQDs exhibited a high fluorescence response specifically for Ca<sup>2+</sup>, with negligible selectivity toward other ions and biomolecules. The CCK8 assay confirmed that the DA-GQDs are nontoxic and biocompatible. Moreover, DA-GQDs have been successfully employed for imaging intracellular Ca<sup>2+</sup>. This study provides new insights into the design of Ca<sup>2+</sup> fluorescence sensors using biocompatible molecular components.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"328 ","pages":"Article 125475"},"PeriodicalIF":4.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qingguo He, Shunping Zang, Yuyang Zeng, Benhua Wang, Xiangzhi Song
{"title":"A bifunctional fluorescent probe for dual-channel detection of H2O2 and HOCl in living cells","authors":"Qingguo He, Shunping Zang, Yuyang Zeng, Benhua Wang, Xiangzhi Song","doi":"10.1016/j.saa.2024.125464","DOIUrl":"10.1016/j.saa.2024.125464","url":null,"abstract":"<div><div>Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and hypochlorous acid (HOCl) are critical reactive oxygen species (ROS) that play significant roles in regulating oxidative stress, closely tied to various human diseases. However, investigating their interplay within living cells has been challenging due to the lack of effective tools for simultaneous discrimination. Herein, we present a bifunctional fluorescent probe, <strong>PTZ-H-H</strong>, capable of simultaneously detecting H<sub>2</sub>O<sub>2</sub> and HOCl in living cells via two distinct fluorescence channels. <strong>PTZ-H-H</strong> exhibits selective and sensitive responses, emitting red fluorescence in the presence of H<sub>2</sub>O<sub>2</sub> and green fluorescence in response to HOCl, with detection limits of 386 nM and 16.8 nM, respectively. The probe was successfully applied in living cells, enabling real-time monitoring of intracellular H<sub>2</sub>O<sub>2</sub> and HOCl. This study demonstrates the potential of <strong>PTZ-H-H</strong> as a powerful tool for exploring the dynamic roles of H<sub>2</sub>O<sub>2</sub> and HOCl in various physiological and pathological processes.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"328 ","pages":"Article 125464"},"PeriodicalIF":4.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Selenophene-containing silicon-rhodamine: A novel near-infrared fluorescent probe for Hg2+ detection and its application in cell imaging","authors":"Xiaochun Wang , Haifeng Yu , Feng Tan","doi":"10.1016/j.saa.2024.125480","DOIUrl":"10.1016/j.saa.2024.125480","url":null,"abstract":"<div><div>Monitoring of mercury ion (Hg<sup>2+</sup>) pollution is one of the eternal themes due to its notorious toxicity. Herein, we elaborately designed a novel fluorescent probe N’-((selenophen-2-yl)methylene)Si-rhodamine B hydrazide (Se-SiRH) by integrating 2-formylselenophene with Si-rhodamine B hydrazide. Se-SiRH exhibited an excellent near-infrared response towards Hg<sup>2+</sup> in MeOH/PBS solution (1:1, <em>v/v</em>, pH = 7.0) and superior specificity towards Hg<sup>2+</sup> than other metal ions. Meanwhile, striking sensitivity towards Hg<sup>2+</sup> was found and the limit of detection in fluorescence measurement was calculated to be 2.1 × 10<sup>−9</sup> mol·L<sup>−1</sup>. What’s more, Hg<sup>2+</sup>-binding mechanism was confirmed with Job-plot measurement, HR-MS and theoretical calculation. Importantly, the success in detecting Hg<sup>2+</sup> in real water samples and visualizing Hg<sup>2+</sup> in HepG2 cells validated its eye-catching application capability in environmental and biological research.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"328 ","pages":"Article 125480"},"PeriodicalIF":4.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}