Development of rare-earth upconversion luminescent nanoprobe for bisulfate sensing and imaging of drug-induced hepatotoxicity

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-07-18 DOI:10.1016/j.saa.2025.126714

Yuting Han, Shufen Pan, Xi Chen, Xiao Ma, Qinglin Chen, Yanyan Tang, Yongquan Wu, Xun Li

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Abstract

Abnormal levels of bisulfite in living organisms may cause respiratory diseases, cardiovascular diseases and even cancer. Rare-earth upconversion luminescent nanomaterials have anti-Stokes luminescence characteristics, excellent photostability and high chemical stability, while organic fluorescent probe has the advantages of the specific recognition groups. Thus, the combination of luminescent nanomaterials and an organic fluorescent probe can offer a novel approach to detect the content of HSO₃⁻. A NIR-excited nanoprobe (UCNP@PEG@HAM) was developed by assembling HSO₃⁻-sensitive dye (HAM) on the surface of upconversion nanoparticles (UCNP) with the assistance of DSPE-PEG₂₀₀₀-NH₂. Upon being excited by light at 980 nm, UCNP@PEG@HAM could achieve upconversion luminescence response to HSO₃⁻ and has the advantages of short response time, high sensitivity (LOD = 0.652 μM) and good selectivity. It could be used not only in vitro applications, but also in fluorescence imaging in mouse liver injury and the remediation models. The nanoprobe can monitor liver injury in living animals using near-infrared excitation. Furthermore, the nanoprobe could be a simple strategy to quickly check whether synthetic drugs can cause liver damage.

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稀土上转换发光纳米探针在亚硫酸氢盐检测和药物肝毒性成像中的应用

亚硫酸盐在生物体中的异常水平可能导致呼吸系统疾病、心血管疾病甚至癌症。稀土上转换发光纳米材料具有抗斯托克斯发光特性、优异的光稳定性和高的化学稳定性，而有机荧光探针具有特异性识别基团的优势。因此，发光纳米材料与有机荧光探针的结合可以提供一种检测HSO3−含量的新方法。在DSPE-PEG2000-NH2的辅助下，将HSO3−敏感染料（HAM）组装在上转化纳米粒子（UCNP）表面，制备了nir激发纳米探针（UCNP@PEG@HAM）。在980 nm的光激发下，UCNP@PEG@HAM可以实现对HSO3−的上转换发光响应，具有响应时间短、灵敏度高（LOD = 0.652 μM）、选择性好等优点。它不仅可以用于体外应用，还可以用于小鼠肝损伤的荧光成像和修复模型。该纳米探针可以利用近红外激发监测活体动物的肝损伤。此外，纳米探针可能是一种简单的策略，可以快速检查合成药物是否会导致肝损伤。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.