Wang Liu , Guoqing Chen , Chaoqun Ma , Lei Li , Taiqun Yang , Chun Zhu , Hui Gao , Anqi Hu , Xingyi Guo , Wenhui Yang , Yuebin Yu , Tingjian Yang , Yali Qian
{"title":"Nitrogen and sulfur co-doped carbon dots for the fluorescence detection of lactose in milk","authors":"Wang Liu , Guoqing Chen , Chaoqun Ma , Lei Li , Taiqun Yang , Chun Zhu , Hui Gao , Anqi Hu , Xingyi Guo , Wenhui Yang , Yuebin Yu , Tingjian Yang , Yali Qian","doi":"10.1016/j.saa.2025.126357","DOIUrl":"10.1016/j.saa.2025.126357","url":null,"abstract":"<div><div>For lactose-intolerant patients, controlling lactose intake is crucial. To address this need, we developed carbon dot-based sensors for lactose detection in milk. Using citric acid and sulfamic acid as precursors, we synthesized nitrogen and sulfur co-doped carbon dots (N,S-CDs) via hydrothermal method. The resulting N,S-CDs exhibit excellent water solubility and fluorescence properties, making them ideal for analytical applications. Lactose can be converted to hydrogen peroxide by the cascade enzyme method of lactase and glucose oxidase. Given the activity of hydrogen peroxide in the Fenton reaction and that the fluorescence of CDs can be significantly quenched by the Fenton reaction, a simple and convenient method for the detection of hydrogen peroxide and lactose was established. The strong oxidizing property of hydroxyl radicals produced in the Fenton reaction destroys the C-O bonds on the carboxylic acid groups of the CDs, which results in the quenched emission peak at 418 nm. The fluorescence intensity of the CDs is linearly related to the concentration of hydrogen peroxide (0.1–5 μM), with a detection limit of 0.09 μM. For lactose in milk, a detection limit of 0.36 μM (0.5–22 μM) was obtained. The method for the detection of hydrogen peroxide and lactose based on the Fenton reaction and N, S co-doped CDs exhibits good stability, specificity, and selectivity. Moreover, the application potential of the method was verified in milk.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126357"},"PeriodicalIF":4.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A novel AIEgen-Based probe Integrated with split aptamer Conjugates for adenosine triphosphate detection in beer","authors":"Lingchen Wang, Yue Huang, Linlin Sun, Xiaojuan Gong, Chuan Dong, Shaomin Shuang","doi":"10.1016/j.saa.2025.126332","DOIUrl":"10.1016/j.saa.2025.126332","url":null,"abstract":"<div><div>Herein, a novel rapid, specific, and sensitive probe for adenosine triphosphate (ATP) detection is developed. We have pioneered the integration of split ATP aptamers with aggregation-induced emission (AIE) via chemical binding to obtain a novel ATP detection probe (TPE probe). Upon the binding of the ATP split aptamer to the target, the intermolecular distance of the AIE molecules decreases, restricting their spatial rotation, which leads to an enhancement of the fluorescence signal, thereby enabling the detection of ATP. This unique design provides the probe with both target-specific recognition capability of the aptamer and sensitive signal-reporting function. Under the optimized experimental conditions, the probe exhibits a linearity range from 1-1000 nM with limit of detection 0.14 nM, displaying a comparative advantage over previously reported methods in terms of sensitivity and selectivity. Additionally, this method demonstrated satisfactory performance for ATP detection in beer samples, with recoveries between 97.5 % to 104.6 %. More importantly, the current research is expected to provide an effective monitoring method for beer quality control.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126332"},"PeriodicalIF":4.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Atindra Kanti Mandal , Tathagata Pal , Suparna Mukherji , Soumyo Mukherji
{"title":"Evanescent wave absorbance based label-free detection of microcystin in water and bodyfluids using polyaniline coated optical fiber","authors":"Atindra Kanti Mandal , Tathagata Pal , Suparna Mukherji , Soumyo Mukherji","doi":"10.1016/j.saa.2025.126350","DOIUrl":"10.1016/j.saa.2025.126350","url":null,"abstract":"<div><div>An increase in the eutrophication of water bodies globally is leading to a significant increase in harmful cyanobacterial blooms, causing contamination of water with cyanotoxins like microcystins. This poses a serious threat to human health and has far-reaching environmental consequences. Prolonged exposure to microcystin can result in oxidative stress, inflammation, liver damage, and potentially lead to liver cancer. The detection of microcystin typically requires sophisticated tests such as LC-MS, which are expensive, require high maintenance, and trained professionals to conduct. This poses a challenge when regular on-site assessment is necessary. In response, a simple and sensitive fiber optic immunosensor was developed by utilising the optical properties of polyaniline over the fiber surface. This evanescent wave absorbance-based sensor could successfully detect microcystin-LR in matrices such as lake water, urine, and serum with a limit of detection ranging from 0.001 µg/L to 0.004 µg/L. The sensor demonstrated a linear response from 0.1 to 1000 µg/L and excellent selectivity. The recovery results ranged from 88 % to 112 % indicating reasonable accuracy.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126350"},"PeriodicalIF":4.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gabrielle Jimenez , Letícia Foiani , Julia Toledo Figueiredo , Mariana Sá Alves , Nayara Sá Rodrigues , Celso Muller Bandeira , Mônica Ghislaine Oliveira Alves , Janete Dias Almeida , Herculano da Silva Martinho
{"title":"Histine, carbohydrates/polysaccharides, and proteins with β-sheet conformation as promising staging markers for oral squamous cell carcinoma","authors":"Gabrielle Jimenez , Letícia Foiani , Julia Toledo Figueiredo , Mariana Sá Alves , Nayara Sá Rodrigues , Celso Muller Bandeira , Mônica Ghislaine Oliveira Alves , Janete Dias Almeida , Herculano da Silva Martinho","doi":"10.1016/j.saa.2025.126296","DOIUrl":"10.1016/j.saa.2025.126296","url":null,"abstract":"<div><div>Cancers of the oral cavity and lip ranks 15<span><math><mi>th</mi></math></span> cancer mortality being oral squamous cell carcinoma (OSCC) the predominant malignant neoplasm in the head and neck region. Liquid optical biopsy using infrared absorption spectroscopy offers rapid and accurate diagnosis of several diseases, including oral cancer. Here we investigated saliva samples from patients with OSCC and healthy individuals using micro-reflectance Fourier-transform infrared absorption spectroscopy. Partial least squares discriminant (PLSDA) and support vector machine (SVM) analysis were employed to mining the set of data. SVM analysis validated the PLSDA findings and presented excellent results with sensitivity of 98.6%, specificity of 97.3%, and area under ROC curve of 0.965. Interestingly we found that bands assigned to histidine, carbohydrates/polysaccharides, and content of proteins with <span><math><mi>β</mi></math></span>-sheet conformation contributed to discrimination among groups. Moreover we found that specific changes in these bands could be related to OSCC staging being promising disease gravity markers.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126296"},"PeriodicalIF":4.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aneta Aniela Kowalska , Marta Czaplicka , Sylwia Berus , Ida Wiśniewska , Agnieszka Jamrozik , Zuzanna Gronkiewicz , Magdalena Data , Wojciech Kukwa , Agnieszka Kamińska
{"title":"Salivary gland tumor detection from saliva to theranostic application of surface-enhanced Raman spectroscopy","authors":"Aneta Aniela Kowalska , Marta Czaplicka , Sylwia Berus , Ida Wiśniewska , Agnieszka Jamrozik , Zuzanna Gronkiewicz , Magdalena Data , Wojciech Kukwa , Agnieszka Kamińska","doi":"10.1016/j.saa.2025.126358","DOIUrl":"10.1016/j.saa.2025.126358","url":null,"abstract":"<div><div>Surface-enhanced Raman spectroscopy (SERS) can be considered a rapid, label-free, nondestructive analytical measurement for tumor detection and theranostic applications, beginning from diagnosis as well as tumor treatment and recovery. SERS of saliva samples collected from patients with salivary gland tumors and healthy controls were used to establish a new tool for fast diagnosis before surgery and in follow-up surgery results. The Partial Least Squares Regression (PLSR) method divided the two analyzed data sets, namely the saliva of control patients and those with salivary gland tumors, with 96 % of explained variables in the first three consecutive factors. The outcome indicates the prediction ability of the analyzed model as the low value of root mean square error (cross-validation; RMSE<sub>(CV)</sub> = 0.11) and high values of R-squared (cross-validation; R<sup>2</sup><sub>(CV)</sub> = 0.95) were obtained. The calibration models were created and optimized using other supervised methods, e.g., partial least squares-discriminant analysis, support vector machine classification, and linear discriminant analysis-principal component analysis. Then, their classification abilities were tested with external samples, achieving impressive accuracy. The study showed that the SERS spectra of the two analyzed classes related to the patient’s disease state showed significant differences, allowing the discrimination between them and identifying the external sample.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126358"},"PeriodicalIF":4.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A novel CNN-LSTM model with attention mechanism for online monitoring of moisture content in fluidized bed granulation process based on near-infrared spectroscopy","authors":"Geng Tian , Jie Zhao , Haibin Qu","doi":"10.1016/j.saa.2025.126361","DOIUrl":"10.1016/j.saa.2025.126361","url":null,"abstract":"<div><div>In the fluidized bed granulation (FBG) process, accurate monitoring of granule moisture content is crucial for quality control. Near-infrared (NIR) spectroscopy is a commonly used method for detecting key attributes of granules in FBG processes. However, its spectral characteristics is susceptible to influences from particle size and other factors. There is a need to optimize preprocessing methods, spectral bands, and model types for the NIR models. To reduce the complexity in traditional modeling approaches, this study proposes a novel deep learning model with attention mechanisms for moisture content. The network architecture, named CNN-LSTM-Attention, integrates the spatial feature extraction capabilities of convolutional neural networks (CNN), the sequence-processing capabilities of long short-term memory (LSTM) networks, and the global correlation capturing capability of self-attention mechanisms. This model possesses both feature band optimization and sequence modeling capabilities, enabling more accurate processing of complex spectral sequence data. The results indicate that compared to traditional partial least squares (PLS) and support vector machine (SVM), the CNN-LSTM-Attention model requires no elaborate preprocessing for NIR spectra and can improve the predictive accuracy. The prediction performance of the proposed algorithm is verified using the NIR datasets. The calibration set achieved an R<sup>2</sup> of 0.9777 and a root mean square error (RMSE) of 0.19, while the validation set yielded an R<sup>2</sup> of 0.9689 and an RMSE of 0.20. The research findings provide theoretical guidance and technical support for the rapid and accurate detection of quality attributes in the FBG process, helping to improve quality control in the granulation process.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126361"},"PeriodicalIF":4.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Monitoring cellular glycolysis pathway kinetics in the extracellular medium using label-free, Raman spectroscopy","authors":"Nitin Patil , Zohreh Mirveis , Hugh J. Byrne","doi":"10.1016/j.saa.2025.126363","DOIUrl":"10.1016/j.saa.2025.126363","url":null,"abstract":"<div><div>This study explored the potential of Raman spectroscopy to holistically monitor the glycolysis pathway kinetics as a function of time through the extracellular medium. Initially, the collinearity of individual metabolites of interest- glucose and lactic acid as a function of their concentration was tested followed by the sensitivity analysis of the approach by elucidating the limits of detection (0.85 mM and 2.8 mM) and quantification (2.5 mM and 9.5 mM) for glucose and lactic acid respectively in the biological range. In the process several datamining approaches were also explored. Finally, the A549 cell culture was used for kinetic spectral acquisition of the extracellular medium mimicking the kinetic glycolysis assay as a function of time under different pathway modulations. The spectra were resolved and fitted with a kinetically constrained-model (A → B → C) using the multivariate curve resolution- alternating least squares tool for all the modulated conditions to elucidate the pathway kinetics and the rate of change. The rate of change of the resolved components for the stimulated condition (k1: 0.005 min<sup>−1</sup>, k2: 0.011 min<sup>−1</sup>) was approximately twice as that of the control (k1: 0,045 min<sup>−1</sup>; k2: 0.049 min<sup>−1</sup>) while the inhibited condition (k1: 0.025 min<sup>−1</sup>, k2: 0.017 min<sup>−1</sup>) was substantially slower. The technique is superior to the targeted current gold standard kinetic assay approach, in that it is holistic in nature and has potential applications in drug discovery, bioprocessing, disease diagnostics, etc. Furthermore, this approach overcomes the limitations of the omics/multiomics approaches, limited to a snapshot of cellular metabolism. This study serves as a guideline for future, more complex subcellular kinetic spectroscopy experiments.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126363"},"PeriodicalIF":4.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Spectral and cytotoxicity studies of hybrid silver nanoparticles in human lymphocytes","authors":"Bojana B. Laban , Uroš Ralević , Andreja Leskovac , Sandra Petrović , Milovan Stoiljković , Milena Rosić , Ana Vujačić Nikezić","doi":"10.1016/j.saa.2025.126360","DOIUrl":"10.1016/j.saa.2025.126360","url":null,"abstract":"<div><div>This study used Raman spectroscopy and genotoxicity/cytotoxicity assays to investigate the synthesized hybrid silver nanoparticles (Ag@LM/ICG NPs) and their effects on human lymphocytes. Raman spectroscopy was employed to obtain detailed chemical information on Ag@LM/ICG NPs and biochemical insights into untreated and Ag@LM/ICG NPs-treated lymphocytes. While average Raman spectra of NPs-treated cells showed no visible changes at the single-cell level, Raman mapping revealed a high background of some spectra, suggesting the presence of NPs within cells and/or NPs-induced cell structure alterations. Complementary cytotoxicity and genotoxicity assessments showed that Ag@LM/ICG NPs are non-cytotoxic and non-genotoxic to human lymphocytes at the tested concentration range, with cell viability exceeding 84%. These findings underscore the safety and potential of Ag@LM/ICG NPs as biocompatible agents for biomedical applications.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126360"},"PeriodicalIF":4.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Design and Synthesis of two novel carbazole-based fluorescent probes and their application in detecting Hg2+","authors":"Huan-Qing Li, Yun Li, Shi-Hang Yang, Ji-Lin Guo, Jian-Yuan Chen, Zhao-Yang Wang","doi":"10.1016/j.saa.2025.126352","DOIUrl":"10.1016/j.saa.2025.126352","url":null,"abstract":"<div><div>Mercury is a highly toxic and non-degradable heavy metal pollutant that poses great harm to human health and the ecological environment. Two novel carbazole based thioacetal fluorescent probes <strong>4a</strong> and <strong>4b</strong> for highly sensitive and selective detection of Hg<sup>2+</sup> have been designed and synthesized. Though both probes <strong>4a</strong> and <strong>4b</strong> can detect Hg<sup>2+</sup> through thioacetal deprotection reaction, due to the difference in structural stability and the AIE effect of probe <strong>4a</strong>, probe <strong>4a</strong> has higher selectivity and sensitivity, with a detection limit as low as 5.1 × 10<sup>-8</sup> M and a response time of approximately 25 s. In addition, probe <strong>4a</strong> can detect Hg<sup>2+</sup> under the solvent systems with high water content, thus we have successfully applied probe <strong>4a</strong> to the detection of Hg<sup>2+</sup> in actual water and soil samples, and used it as test strips.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126352"},"PeriodicalIF":4.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The characterization and performance of a core–shell structured nanoplatform for fluorescence turn-on sensing and selective removal of perfluorooctane substance","authors":"Zhihai Gong , Ji Li , Fuzhong Wu","doi":"10.1016/j.saa.2025.126356","DOIUrl":"10.1016/j.saa.2025.126356","url":null,"abstract":"<div><div>The high dissociation energy of C-F bonds makes the natural degradation and decomposition of PFAS (per- and poly-fluoroalkyl substances) nearly impossible, leaving the PFAS wastes widely dispersed and permanently persisting in the natural environment. Consequently, detection and recognition techniques, as well as treatment technology, are highly desired for perfluorooctane sulfonate (PFOS) pollutants. In this work, we designed a core–shell magnetic-porous composite structure (denoted as Fe<sub>3</sub>O<sub>4</sub>@MCM-41/EY) for the sensing, adsorption, and removal of PFOS, using EY:CTAB (0.5 μM:80 μM, EY = Eosin Y, CTAB = cetyltrimethylammonium bromide) as the sensing probe, Fe<sub>3</sub>O<sub>4</sub> nanoparticles as the core, and porous silica MCM-41 as the shell. The composite structure of Fe<sub>3</sub>O<sub>4</sub>@MCM-41/EY was confirmed by means of SEM (scanning electron microscope), magnetism, XRD (X-ray diffraction), N<sub>2</sub> adsorption/desorption, and TGA (thermal gravimetric analysis). A probe loading level of 28.5 % was determined. The probe showed an emission turn-on effect toward PFOS owing to the viscosity variation of the micelles caused by PFOS. A linear fitting equation was obtained as I/I<sub>0</sub> = 1.081 + 0.144 × 10<sup>6</sup>[PFOS], R<sup>2</sup> = 0.990, with good sensing selectivity and LOD (limit of detection) of 0.27 μM. In addition, Fe<sub>3</sub>O<sub>4</sub>@MCM-41/EY removed PFOS efficiently with an adsorption capacity of 0.126 mg/g. The novelty of this work was the simultaneous sensing, adsorption, and removal of PFOS using a core–shell magnetic-porous composite structure.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"340 ","pages":"Article 126356"},"PeriodicalIF":4.3,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}