Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy最新文献

{"title":"Graphene quantum dot-enhanced Chemiluminescence sensor based on surface molecular imprinting recognition for the detection of Tetrabromobisphenol A","authors":"AnYuan Tong,&nbsp;Nan Xu,&nbsp;Ting Yan,&nbsp;FuBin Chen,&nbsp;ChunYan Yang","doi":"10.1016/j.saa.2025.126981","DOIUrl":"10.1016/j.saa.2025.126981","url":null,"abstract":"<div><div>Herein, silica nanoparticles were employed as the carrier for surface molecular imprinted polymer with high selectivity for Tetrabromobisphenol A (TBBPA). Nitrogen-doped graphene quantum dots (NGQDs) were incorporated to enhance the chemiluminescence intensity of the Luminol-H₂O₂ system, as TBBPA was found to effectively suppress the chemiluminescence signal of this system. Based on these principles, a molecular imprinting technique-chemiluminescence (MIT-CL) sensor was developed by integrating the NGQDs-sensitized Luminol-H₂O₂ chemiluminescence system with the specific recognition material SiO₂@TBBPA MIP was constructed to detect TBBPA in environmental water samples. Under the optimal conditions, the sensor exhibited a linear detection range of 8.0 × 10⁻¹⁰-2.0 × 10⁻⁸ mol/L, with the detection limit (3σ/k) of 3.2 × 10⁻¹¹ mol/L. The illustrated MIT-CL method was utilized for the detection of TBBPA in water samples, yielding satisfactory recovery rates ranging from 96.25 % to 107.50 %.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126981"},"PeriodicalIF":4.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IC-MAC: A novel NIR fluorescent probe for monitoring the glutathione fluctuations in drug-induced liver injury","authors":"Xiao Li ,&nbsp;Chengqing Zhao ,&nbsp;Zhiyuan Wang ,&nbsp;Suntao Shi ,&nbsp;Huiling Gao ,&nbsp;Jiang Wu ,&nbsp;Baoxin Zhang","doi":"10.1016/j.saa.2025.126980","DOIUrl":"10.1016/j.saa.2025.126980","url":null,"abstract":"<div><div>Drug-induced liver injury (DILI) is widely recognized as a major threat to global public health, posing significant obstacles to clinical treatment and drug innovation. The core mechanism underlying DILI progression is the depletion of intracellular glutathione (GSH), which is widely regarded as a key marker of oxidative liver injury and an important biomarker for early-stage DILI. In this study, we developed <strong>IC-MAC</strong>, a novel near-infrared (NIR) fluorescent probe for the highly sensitive and selective detection of GSH, which exhibited excellent photostability, a large Stokes Shift, and a significant fluorescence enhancement upon reaction with GSH. The probe demonstrated high biocompatibility and enabled real-time imaging of intracellular GSH fluctuations under physiological and oxidative stress conditions. In a mice model of acetaminophen-induced DILI, <strong>IC-MAC</strong> enabled dynamic visualization of GSH depletion in the liver and its recovery following therapeutic intervention. Fluorescence intensity was significantly correlated with the degree of GSH depletion and treatment response, providing robust evidence for the quantitative assessment of liver injury severity and drug efficacy. Collectively, these findings highlighted the potential of <strong>IC-MAC</strong> as a powerful platform for early DILI diagnosis and <em>in vivo</em> redox monitoring.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126980"},"PeriodicalIF":4.6,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A thiol-activated near-infrared fluorescent H2S donor for visualizing H2S release and its applications","authors":"Jicheng Wang ,&nbsp;Xuan Wang ,&nbsp;Nianwei Wang ,&nbsp;Yikun Ren ,&nbsp;Lin Dong ,&nbsp;Huarong Xu ,&nbsp;Huiming Hua ,&nbsp;Dahong Li","doi":"10.1016/j.saa.2025.126976","DOIUrl":"10.1016/j.saa.2025.126976","url":null,"abstract":"<div><div>Hydrogen sulfide (H₂S), a critical endogenous signaling molecule, is involved in diverse physiological processes, and H₂S donors have emerged as promising therapeutic agents. While organic donors address the uncontrolled release limitations of inorganic counterparts, most thiol-activated systems depend exclusively on cysteine (Cys), risking cellular thiol imbalance. To overcome this limitation, we developed <strong>AD-SH</strong>, a novel benzopyran salts-based NIR fluorescent H₂S donor responsive to multiple biothiols. <strong>AD-SH</strong> exhibits high selectivity and sensitivity toward all intracellular biothiols (e.g., cysteine, glutathione, and homocysteine) without preferentially depleting a single Cys. Importantly, its near-infrared (NIR) fluorescence enables real-time tracking and visualization of H₂S release. With excellent biocompatibility, photostability, and in vivo imaging performance in mice, <strong>AD-SH</strong> demonstrates its strong potential for live-cell imaging and dynamic H₂S release tracking.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126976"},"PeriodicalIF":4.6,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical force-induced luminescence enhancement and Chromism of donor-acceptor Tetraphenylethylene-naphthalene Chalcone isomers","authors":"Hanrong Liu ,&nbsp;Huizhuan Zhu ,&nbsp;Hui Wang ,&nbsp;Baobei Liu ,&nbsp;Jiakun Bai ,&nbsp;Huijuan Zhang ,&nbsp;Junhui Jia","doi":"10.1016/j.saa.2025.126978","DOIUrl":"10.1016/j.saa.2025.126978","url":null,"abstract":"<div><div>Two new donor-acceptor (D-A) tetraphenylethylene-naphthalene chalcone isomers, (<em>E</em>)-1-(naphthalen-2-yl)-3-(4-(1,2,2-triphenylvinyl)phenyl) prop-2-en-1-one (<strong>1N3T</strong>) and (<em>E</em>)-3-(naphthalen-2-yl)-1-(4-(1,2,2-triphenylvinyl)phenyl)prop-2-en-1-one (<strong>3N1T</strong>), were designed and synthesized to investigate their structure-dependent photophysical properties, aggregation-induced emission enhancement (AIEE), and mechanoresponsive behaviors. These two isomers feature tetraphenylethylene (TPE), a well-known aggregation-induced emission luminogen (AIEgen), coupled with a naphthalene chalcone moiety, where the positional isomerism arises from the distinct spatial arrangement of donor (naphthalene) and acceptor (chalcone) groups. Systematic characterization revealed intramolecular charge transfer (ICT) characteristics in solution, as evidenced by solvent-dependent fluorescence emission spectra. Density functional theory (DFT) calculations corroborated the ICT mechanism, demonstrating HOMO-LUMO localization patterns that align with the D-A configurations. Notably, both isomers exhibited exceptional mechanochromic sensitivity. Grinding-induced amorphization led to a remarkable enhancement in solid-state fluorescence quantum yields (QYs), increasing by 27-fold (<strong>1N3T</strong>) and 79-fold (<strong>3N1T</strong>). Powder X-ray diffraction (PXRD) and field emission scanning electron microscopy (FESEM) confirmed a reversible crystalline-to-amorphous phase transition, with the disordered amorphous state suppressing non-radiative decay pathways (e.g., π-π interactions) and unlocking enhanced emission. The superior mechanoresponsiveness of <strong>3N1T</strong> was attributed to its optimized D-A geometry, which facilitates efficient structural disruption under mechanical stress. This work elucidates critical structure-property relationships governing AIEE and mechanochromism, emphasizing the role of positional isomerism in modulating solid-state packing and stimuli-responsive luminescence. The findings advance the rational design of adaptive optoelectronic materials for applications in pressure-sensitive sensors, smart coatings, and rewritable optical storage systems.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126978"},"PeriodicalIF":4.6,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A two-emitting dye-embedded fluorescent sensor based on zirconium MOF for effective detection of fluoride","authors":"Yaqiong Zhang ,&nbsp;Junping Lv ,&nbsp;Qin Zhou ,&nbsp;Kaile Chu ,&nbsp;Min Li ,&nbsp;Yayuan Li ,&nbsp;Jie An ,&nbsp;Zhifang Wu ,&nbsp;Sijin Li","doi":"10.1016/j.saa.2025.126949","DOIUrl":"10.1016/j.saa.2025.126949","url":null,"abstract":"<div><div>Ratiometric fluorescent (RF) sensors, leveraging their inherent calibration mechanism, can greatly reduce the interference of irrelevant factors and exhibits superior detection sensitivity and more attractive visual detection effects in the process of quantitative analysis. Up to now, there are few probes that can detect fluorine by the proportional fluorescence signal. Herein, we constructed a RF probe for determination of fluorine by confining self-synthesized red emitting organic small molecules 10-(diethylamino)-3-hydroxy-5,6-dihydrobenzo[<em>c</em>]xanthen-12-ium (HTD) into the microchannels and cavity structures of UiO-66-NH₂, exhibiting two emission maxima at 428 nm and 616 nm, respectively. After introducing fluoride into the system of probe, a drastic fluorescence enhancement at 428 nm was emerged, whereas the emission peak at 616 nm underwent minor fluctuations. The probe that has been developed enables the sensitive and selective detection of fluoride ions, exhibiting excellent linear relationships in the range of 0–100 μM. The detection limit value was estimated to be 6.93 μM. This unique ratiometric luminescence behavior of probe toward fluoride resulted from the creation of hydrogen bonds between fluoride and amino groups in HTD@UiO-66-NH₂. Notably, the probe could utilize as an effective tool for detecting fluoride in cells with high-quality results, which could have important implications for research in biology, toxicology, and related fields. Simultaneously, its corresponding portable online test strips can be easily acquired and employed for fluoride detection through visual colorimetric analysis. The proposed method offers an alternative technology for quantifying fluoride levels in real-life water samples.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126949"},"PeriodicalIF":4.6,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyridine-hydrazone-based charge-transfer probe for detecting Cu2+ and Co2+ ions in aqueous solutions","authors":"Jieun Park ,&nbsp;Dong In Moon ,&nbsp;Bulle Shah ,&nbsp;Narinder Singh ,&nbsp;Doo Ok Jang","doi":"10.1016/j.saa.2025.126979","DOIUrl":"10.1016/j.saa.2025.126979","url":null,"abstract":"<div><div>In the field of environmental monitoring, there remain considerable challenges regarding the development of selective chemosensors with high sensitivity for detecting transition metal ions in aqueous media. In this study, we prepared and investigated 1,3-bis((2-((<em>E</em>)-(2-(pyridin-2-yl)hydrazineylidene)methyl)-1H-pyrrol-1-yl)methyl) benzene as a pyridine-hydrazone-based probe (receptor <strong>A</strong>) for the selective detection of metal ions. Upon interacting with Cu²⁺ and Co²⁺ ions, the probe responded with significant optical changes, thereby facilitating simple ion detection. The ultraviolet-visible absorption maximum of the receptor <strong>A</strong> underwent a remarkable hypsochromic shift to 291 nm upon the addition of Cu²⁺ solution, while the addition of Co²⁺ solution led to a bathochromic shift to 426 nm. Receptor <strong>A</strong> was capable of selectively detecting Cu²⁺ and Co²⁺ in aqueous solutions without any marked interference from other metal ions, with respective limits of detection of 2.12 × 10⁻⁶ and 3.47 × 10⁻⁸ M, which are significantly lower than the WHO guidelines. The ability of receptor <strong>A</strong> to detect Cu²⁺ and Co²⁺ in real water samples was also demonstrated. These findings highlight the potential utility of pyridine-hydrazones as an effective probe for real-time monitoring of Cu²⁺ and Co²⁺ ions in environmental samples.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126979"},"PeriodicalIF":4.6,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-assisted Raman spectroscopy for enhanced plastic identification","authors":"Szymon Wójcik ,&nbsp;Magdalena Król ,&nbsp;Paweł Stoch","doi":"10.1016/j.saa.2025.126973","DOIUrl":"10.1016/j.saa.2025.126973","url":null,"abstract":"<div><div>The escalating global issue of plastic waste, with over 380 million tons produced annually and recycling rates at a mere 9 %, necessitates more effective identification and sorting methods. Conventional approaches struggle with visually similar polymers, reducing the efficiency of mechanical recycling. In this study, we aimed to develop a methodological framework for accurate plastic classification by combining machine learning with handheld Raman spectroscopy. A dataset of 3000 spectra was collected from 10 common plastic types (PET, HDPE, PVC, LDPE, PP, PS, ABS, PC, PLA, PTFE) under varied measurement conditions. Raman spectroscopy provides unique molecular fingerprints for different plastics, while machine learning enhances classification accuracy, particularly for complex mixtures or contaminated samples encountered in recycling streams.</div><div>To address the classification challenge, we proposed a branched neural network architecture (Branched PCA-Net), inspired by Deep&amp;Wide design and operating on Principal Component Analysis (PCA) reduced spectral data. This network employs separate paths for high-, medium-, and low-variance principal components prior to final classification. The model achieved over 99 % accuracy on the test dataset, with perfect classification for 7 out of 10 plastics and high accuracy for the remaining three. Robustness was further validated on new samples measured under different conditions, confirming strong generalization capabilities.</div><div>This study provides a methodological contribution through the introduction of the Branched PCA-Net architecture, which shows high potential for spectroscopic data analysis. While not directly scalable to high-throughput sorting, the approach offers significant promise for quality control and targeted plastic identification in recycling workflows.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126973"},"PeriodicalIF":4.6,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FRET-driven fluorescent aptasensor for ultrasensitive low-density lipoprotein detection integration of sulfur-doped carbon nitride quantum dots and MXene-Gold nanocomposites","authors":"Guiyin Li ,&nbsp;Cunhong Cen ,&nbsp;Junan He ,&nbsp;Wei He ,&nbsp;Jincai Xie ,&nbsp;Lingling Fan ,&nbsp;Xiaohong Tan ,&nbsp;Yong Huang","doi":"10.1016/j.saa.2025.126970","DOIUrl":"10.1016/j.saa.2025.126970","url":null,"abstract":"<div><div>Low-density lipoprotein (LDL) is a critical biomarker for cardiovascular disease (CVD) risk assessment. Accurate and sensitive monitoring of LDL levels in human serum is crucial for timely diagnosis, targeted intervention, and reduction of mortality. In this paper, we constructed a fluorescent aptamer sensor for LDL determination based on sulfur-doped graphitic carbon nitride quantum dots conjugated with LDL aptamer (S-g-CNQDs-LDL_Apt) as a fluorescence donor and MXene-supported gold nanoparticles (MXene-Au) as the energy acceptor, based on fluorescence resonance energy transfer (FRET) principle. The sensing mechanism relies on LDL concentration-dependent fluorescence recovery mediated by the disruption of FRET. Under optimal conditions, the fluorescent aptamer sensor exhibited a linear dynamic range of 20.0–100.0 μg/mL (regression equation: Y = 0.00874× – 0.07426, R² = 0.9900), with a detection limit (LOD) of 0.343 μg/mL. In clinical serum samples, the relative standard deviations (RSD) ranged from 0.48 % to 2.64 %, confirming the sensor's reliability for LDL analysis in biological matrices. The proposed biosensor demonstrates great potential for clinical serum analysis, offering a novel paradigm for point-of-care CVD risk stratification.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126970"},"PeriodicalIF":4.6,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A materialized intelligent sensing platform driven by ultra-sensitive fluorescent probe: Smart phone-assisted portable detection of SO2 and its derivatives","authors":"Chenxiao Wang ,&nbsp;Qiutong Chen ,&nbsp;Mingyu Tian ,&nbsp;Lin Wu ,&nbsp;Tianyu Liang ,&nbsp;Chengyan Wu ,&nbsp;Yang Li ,&nbsp;Keli Zhong ,&nbsp;Lijun Tang","doi":"10.1016/j.saa.2025.126974","DOIUrl":"10.1016/j.saa.2025.126974","url":null,"abstract":"<div><div>Sulfur dioxide (SO₂) has long been recognized as an environmental pollutant, and excessive intake of SO₂ and its derivatives (HSO₃⁻/SO₃²⁻) is harmful to human health. In this paper, we report a specific recognition of HSO₃⁻ fluorescent probe <strong>RQB-RP</strong>, which exhibits color-enhancing and strong fluorescence activation, good water solubility, fast response (4 s), high fluorescence quantum efficiency (<em>Ф</em> = 21.87 %) and high sensitivity (detection limit = 120.66 nM), and has a strong potential for application. Furthermore, the probe <strong>RQB-RP</strong> was materialized and combined with a smartphone sensing platform to achieve on-site visual quantitative detection of HSO₃⁻ without the need for large-scale instrumentation. The <strong>RQB-RP</strong> also has the ability to accurately detect HSO₃⁻ levels in food and Chinese medicine samples to assess compliance and safety. Furthermore, <strong>RQB-RP</strong>'s excellent biocompatibility has been successfully used for the in situ detection of endogenous and exogenous HSO₃⁻ in mitochondria of MCF-7 cells.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126974"},"PeriodicalIF":4.6,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AuNSs@Ag Nanoprobe-based dual-mode sensor for rapid and sensitive detection of dimethoate","authors":"Mengling Hu ,&nbsp;Junjie Qi ,&nbsp;Guoyong Jiang ,&nbsp;Jingkun Li ,&nbsp;Jiahua Wang ,&nbsp;Yuqi Wan ,&nbsp;Fuwei Pi","doi":"10.1016/j.saa.2025.126972","DOIUrl":"10.1016/j.saa.2025.126972","url":null,"abstract":"<div><div>Dimethoate (DMT), an organophosphate pesticide, is extensively employed in agriculture for pest management, however its persistent residues raise critical concerns regarding human health and environmental safety, necessitating the development of reliable quantification methods for residual monitoring. The sensor utilizes silver-coated gold nanostructures (AuNSs@Ag), which possess bimetallic properties and intrinsic peroxidase-like activity, to catalyze the H₂O₂-mediated oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). This reaction results in the formation of a blue oxidation product (ox-TMB), producing a distinct colorimetric signal. Simultaneously, this reaction generates a characteristic SERS fingerprint spectrum of ox-TMB. However, upon the introduction of DMT, competitive adsorption occurs between DMT and TMB for the active sites on the surface of AuNSs@Ag, resulting in a reduction of the reactive sites on AuNSs@Ag and a decrease in its peroxidase-like activity. Consequently, the formation of ox-TMB is reduced, leading to a diminished transition from the colorless to the blue state, as well as a weakened SERS signal. Therefore, this dual-mode probe enables specific and highly sensitive detection of DMT. The limit of detection (LOD) for DMT was determined to be 6.76 μg/L using the colorimetric method and 1.19 μg/L using the SERS method. In real sample applications, such as in apples and celery, the recovery rates ranged from 88.33 % to 116.67 %, with relative standard deviations (RSD) below 5 %. This dual-mode sensor offers a simple, rapid, and cost-effective approach for on-site detection of DMT, presenting significant potential for applications in food safety monitoring and environmental protection.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"347 ","pages":"Article 126972"},"PeriodicalIF":4.6,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}