Sensors and Actuators B: Chemical最新文献

{"title":"Efficacy evaluation of edaravone by imaging of •OH in cerebral ischemia-reperfusion injury with a novel fluorescent probe","authors":"Hanyue Xiang, Ayijiang Taledaohan, Mengshan Yu, Bingxuan Huang, Kai Wang, Wenzhuo Fu, Nao Xiao, Chunlin Li, Yuji Wang","doi":"10.1016/j.snb.2024.137163","DOIUrl":"https://doi.org/10.1016/j.snb.2024.137163","url":null,"abstract":"Hydroxyl radical (•OH) is a crucial target during the course of stroke. Here, we developed a novel, blood-brain barrier (BBB) permeable fluorescent probe <strong>BIBP-H</strong> with hydrohemicyanine moiety for intelligent detection of •OH. The hydrogen in <strong>BIBP-H</strong> is abstracted by •OH to release the caged large π-conjugation fluorophore, triggering a turn-on fluorescence response. With high selectivity, sensitivity and biocompatibility, <strong>BIBP-H</strong> can monitor both endogenous and exogenous •OH in living neuron cells, and can also visualize cerebral •OH in situ in stroke rats modeled by cerebral ischemia-reperfusion injury (CIRI). Using the probe, we further corroborated the anti-apoplexy efficacy of edaravone (EDA) to reduce •OH levels. These findings reveal that <strong>BIBP-H</strong> is a convenient and reliable probe for •OH bioimaging and stroke monitoring.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"31 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142867380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-tandem enzymatic modules-encapsulated metal-organic framework cubes with mutual-validated operation for dual-modal competitive immunoassay of antibiotics","authors":"Yi Yang, Licheng Yu, Liang He, Pengli Bai, Xiwen He, Langxing Chen, Yukui Zhang","doi":"10.1016/j.snb.2024.137160","DOIUrl":"https://doi.org/10.1016/j.snb.2024.137160","url":null,"abstract":"Adaptive fusion of nanoscale metal-organic frameworks (nMOFs) and enzyme-based immunoassay is an insightful combination for inspired establishment of antibiotic assay, typically in a competitive format, in which inherent enzymes in nMOFs can serve as generators for amplifying catalytic signals. Albeit important, there is currently a lack of explorations of enzymatic signal outputs with multiple modalities in typical competitive immunoassays, which may face the false signals and lack cross-validation. Herein, non-tandem bi-enzymatic modules such as urease and horseradish peroxidase were co-encapsulated into nMOF cubes and functionalized with antibodies into entities for dual-modal competitive immunoassay of oxytetracycline (OTC), which can convert the bi-enzymatic catalytic signals into fluorogenic and chromogenic reflection and can gain the mutual validation merits. The fluorescence mode can achieve an OTC detection range from 5<!-- --> <!-- -->ng/mL to 2000 ng/mL, with a detection limit (LOD) of 2.14<!-- --> <!-- -->ng/mL; complementally, the absorbance mode can achieve a relatively narrow detection range from 1<!-- --> <!-- -->ng/mL to 200<!-- --> <!-- -->ng/mL, but a lower LOD of 0.32<!-- --> <!-- -->ng/mL. The dual-mode sensing parameters of the competitive immunoassay in responding to OTC analysis differed in terms of the linear range and detection limit, which can be integrated to offer reciprocal authentication and expand the detection diversity. Overall, our proposed method may provide a new idea in dual-modal competitive immunoassay of antibiotics, signifying its promising expansion for rational substitution of enzymatic modules and its potential performance in wide applications.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"261 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142858069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A MXene-Au Nanosheets-based Fluorescent-SERS Dual-Mode Biosensor Integrated with CRISPR/Cas12a System for Endotoxin Detection","authors":"Honglian Wu, Ruolin Zhang, Huang Lai, Lai luogen, Yi Liu, Yadi Fan, Yu Zhang, Jingyu Shi, Mo Yang","doi":"10.1016/j.snb.2024.137120","DOIUrl":"https://doi.org/10.1016/j.snb.2024.137120","url":null,"abstract":"Endotoxins or lipopolysaccharides (LPS) are potent inflammatory agents that pose serious health risks even at low concentrations, thus requiring sensitive and reliable detection methods. Current methods for endotoxin detection, primarily the Limulus Amebocyte Lysate (LAL) assay and Enzyme-linked Immunosorbent Assays (ELISA) are limited by complex procedures, prolonged assay time, and susceptibility to interference. To address these challenges, we developed a single-reporter probe-based dual-mode biosensor integrating MXene-Au nanosheets with the CRISPR/Cas12a system for endotoxin detection. The sensing platform was constructed by conjugating ssDNA-Cy5 reporters onto MXene-Au nanosheets. MXene-Au nanosheets served dual functions, including efficient quenching of fluorescence and strong enhancement of SERS signals. In the presence of target endotoxins, CRISPR/Cas12a remains inactive, leaving the ssDNA-Cy5 reporters intact on the MXene-Au surface. The close proximity of Cy5 to the MXene-Au surface results in quenched fluorescence (“FL off”) but enhanced SERS signals (“SERS on”). Conversely, in the absence of endotoxins, activated CRISPR/Cas12a cleaves the ssDNA-Cy5, releasing Cy5 from the surface, thus leading to fluorescence recovery (“FL on”) but diminished SERS signals (“SERS off”). By measuring the changes in SERS and FL signals and analysing the ratiometric I_SERS/I_FL signals, the biosensor achieved a lower limit of detection (LOD) of 15.9<!-- --> <!-- -->pg/mL within 30<!-- --> <!-- -->minutes compared to single detection modes. Validation studies in complex matrices, including spiked water and milk samples, showed performance comparable to commercial endotoxin detection kits. Overall, our CRISPR-Cas12a-mediated fluorescent-SERS dual-mode biosensor not only improves detection sensitivity but also provides built-in quality control, improving the reliability of results through cross-validation.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"23 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-gas sensor for ultra-close overlapping spectra based on second harmonic peak shift","authors":"Huifang Gao, Qing Yang, Qianjin Wang, Zhirong Zhang, Yuangang Lu, Le Wang","doi":"10.1016/j.snb.2024.137159","DOIUrl":"https://doi.org/10.1016/j.snb.2024.137159","url":null,"abstract":"In spectral absorption techniques, overlapping spectra cause distortion in absorption line heights, leading to inaccuracies in concentration measurements, which poses a significant challenge for measuring multi-component gases. In this paper, an innovative method that utilizes the displacement of the second harmonic (<em>2f</em>) peak to mitigate <em>2f</em> height errors resulting from cross-interference is presented. Our research findings reveal that cross-interference distorts symmetrical absorption spectra, and by adjusting the modulation depth, a predictable shift in the <em>2f</em> peak can be induced, with the magnitude of this shift directly correlated to the concentration of the interfering gas. This discovery enables us to correct <em>2f</em> height errors, thereby enabling precise concurrent analysis of multi-component gases while mitigating the effects of cross-interference. This approach holds promise in addressing the challenge of accurate dual-parameter gas measurement in scenarios of ultra-close spectral overlap. In a proof-of-concept experiment, we selected C₂H₂ and CO₂ (two gases with closely spaced and overlapped absorption spectra) as example gases for detection. Notably, this method achieved high-precision measurements with an accuracy of 27 ppb for C₂H₂ and 5 ppm for CO₂ when dealing with ultra-close spectra of 1 ppm C₂H₂ and 4350 ppm CO₂, whose centerline offset is merely 0.0859 cm⁻¹. This represents a 15-fold improvement in measurement accuracy for C₂H₂. The minimum detection limits for C₂H₂ and CO₂ are 3.2 ppb and 13.6 ppm, respectively. Furthermore, this work offers a novel solution to mitigate cross-interference, demonstrating good versatility for application in other absorption spectroscopy techniques without additional costs or structural changes.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"39 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-site peroxidase-mimic graphdiyne-based colorimetric sensor arrays with machine learning for screening of multiple antibiotics","authors":"Xingchen Qiu, Jianyu Yang, Rui Bai, Mengdi Zhao, Changfa Shao, Qingqing Zhao, Yu Gu, Chunxian Guo, Chang-Ming Li","doi":"10.1016/j.snb.2024.137158","DOIUrl":"https://doi.org/10.1016/j.snb.2024.137158","url":null,"abstract":"The abuse of antibiotics poses a significant threat to both human health and the ecosystem, while the rapid screening of multiple antibiotics remains a challenge. We report the design of a dual-site peroxidase (POD)-mimic nanozyme comprising self-assembled hemin molecules and Cu²⁺ on graphdiyne (GDY) for screening of multiple antibiotics assisted with machine learning (ML). Cu ions can bond with π bonds and carbonyl groups on the surface of GDY, thereby enabling strong interface of hemin and GDY for an enhanced generation of hydroxyl radicals (^{<strong>.</strong>}OH). GDY/Hemin/Cu exhibits POD-like activity in wide pH conditions and temperatures ranging from 20 to 70 °C. With the assistance of ML, GDY/Hemin/Cu-based colorimetric sensor arrays demonstrate fast and accurate identification of multiple antibiotics including kanamycin, norfloxacin, ampicillin sodium, catechol and isoniazid. Theoretical calculation confirms that strong binding affinity enables specificity of the GDY/Hemin/Cu towards antibiotics. By employing support vector machine algorithm to assess antibiotic content, a high detection accuracy of 97.5% is achieved across 40 honey samples, underscoring the potential practical applications in screening of multiple antibiotics.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"91 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142848950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atmospheric Pressure Difference Centrifuge for Stable and Consistent Plasma Separation","authors":"Tonghuan Zhan, Shuqiang Min, Xianchang Wu, Yulun Wu, Yange Huang, Jiahao Liu, Zhihao Chen, Hui Niu, Bing Xu","doi":"10.1016/j.snb.2024.137143","DOIUrl":"https://doi.org/10.1016/j.snb.2024.137143","url":null,"abstract":"Separating plasma from micro-scale whole blood is a necessary step for point-of-care hematology diagnosis and therapy. Current manual centrifuges offer an affordable, simple, and electricity-free method for plasma separation with low sample requirement. However, variations in manual operations and user errors often lead to inconsistent separation efficiency and unreliable results. Inspired by the constant atmospheric pressure, we develop an electricity-free centrifuge (henceforth called ‘Atmosfuge’) with consistent separation efficiency and minimal separation variations. The Atmosfuge comprised a sealed syringe and a gear transmission system. During plasma separation, the syringe rod was first pulled to create a vaccum in the barrel. Upon releasing, constant atmospheric pressure difference between internal air pressure of the barrel and the external atmospheric pressure would push the rod and drove the gear, rotating the blood sample for plasma separation. It ensures a uniform rotating speed (~2300<!-- --> <!-- -->rpm) across different users. By harnessing the Boycott effect and adjusting the centrifugal radius, we can achieve a plasma yield of ~93% and purity of ~99.99% in separating 15 μL whole blood within 250<!-- --> <!-- -->s. In addition, we integrated the Atmosfuge with a calibration ruler for visible and accurate hematocrit measurement. Finally, the separated plasma were tested for blood glucose detection by integrating microfluidic cloth-based analytical devices (μCADs) and colorimetric assays. Given its consistent separation efficiency and simple operation, Atmosfuge present a cost-effective alternative to traditional centrifuges, particularly in medical resource-limited regions.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"17 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel based Flexible wearable sweat sensor for SERS-AI monitoring treatment effect of Lung Cancer","authors":"Zhaoxian Chen, Shihong Liu, Wenrou Yu, Li Wang, Fengxue Lv, Liejun Yang, Huiqing Yu, Haiyang Shi, Yingzhou Huang","doi":"10.1016/j.snb.2024.137155","DOIUrl":"https://doi.org/10.1016/j.snb.2024.137155","url":null,"abstract":"Comfortable treatment of malignant tumors is the clinical orientation of cancer therapy at present, which puts forward a high demand for non-invasive, portable and high-frequency monitoring status of tumor in the treatment. Unlike traditional blood and X-ray technique, here we have developed hydrogel-based wearable sweat sensors, equipped with multiple molecular receptors for surface enhanced Raman spectroscopy (SERS) monitoring treatment effects of lung cancer. The SERS technique was utilized in combination with multiple artificial intelligence (AI) algorithms (LGB GNB, LDA, RF, and SVM) to develop a novel and precise diagnostic model for monitoring the treatment effect. Based on 12617 SERS spectras from clinical patients, the results successfully diagnosed three treatment effects (progressive disease, partial response, and no change) with an accuracy of 89.7%. Benefiting from data mining in AI algorithms, key Raman spectra features in clinical spectra are identified to explore characteristic biomarkers of lung cancer associated with various comorbidities. The clinical data suggest that carbonyl biomarkers in sweat might be crucial for understanding complications such as diabetes and hypertension. Our results not only offer a novel and comfortable monitoring technique but also enable personalized treatment of lung cancer with complications, presenting significant potential for clinical application.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"12 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent probes based on aza-Nile Red for visualizing mitochondrial polarity fluctuation","authors":"Hui Wang, Guo-Wei Chen, Hong-Xia Wang, Ru Sun, Jian-Feng Ge","doi":"10.1016/j.snb.2024.137151","DOIUrl":"https://doi.org/10.1016/j.snb.2024.137151","url":null,"abstract":"Nile Red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Whereupon, three neutral red/near-infrared fluorescent dyes <strong>1a-1c</strong> with good water solubility were synthesized by introducing nitrogen atoms into the 2, 3 and 4 positions of Nile Red in order to improve the biocompatibility in this paper. Optical testing showed that the maximum absorption wavelengths of <strong>1a-1c</strong> were in the range of 525–617<!-- --> <!-- -->nm, while the maximum emission ones located at 584–668<!-- --> <!-- -->nm in different solvents. When the solvent polarity (<em>Δf</em>) was reduced from 0.316 to 0.020, the maximum absorption wavelengths of <strong>1a-1c</strong> were blue-shifted by 73–97<!-- --> <!-- -->nm, and the fluorescence intensities were enhanced by 32–44 folds. Meanwhile, excellent linear relationships between the fluorescence lifetimes, absolute fluorescence quantum yields of <strong>1a-1c</strong> and <em>Δf</em> (0.229–0.307) were established with high correlation coefficients (R² = 0.98–0.99). In addition to excellent low toxicity and mitochondrial targeting properties, <strong>1a-1c</strong> were able to detect polarity changes in models of inflammation induced by Lipopolysaccharide at 50<!-- --> <!-- -->nM. Furthermore, based on the significant mitochondrial polarity differences between cancer and normal cells, <strong>1a-1c</strong> had the ability to differentiate them. And <strong>1a-1c</strong> also were capable of real-time monitoring mitochondrial polarity during starvation or rapamycin-induced autophagy. Overall, the excellent optical properties and biocompatibility of aza-Nile Red (<strong>1a-1c)</strong> suggest that they have great potential in studying mitochondrial physiology and pathological biological processes.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"63 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palladium Hexagonal Nanoparticles/Carbon Nanotubes/Cellulose Nanocrystals Integrated over Paper for Wearable Hydrogen Sensing with Fast Response by Bridging Behavior","authors":"Zhenxu Li, Dongliang Feng, Zhu Zhang, Xinhua Zhao, Xiaxia Xing, Dachi Yang","doi":"10.1016/j.snb.2024.137153","DOIUrl":"https://doi.org/10.1016/j.snb.2024.137153","url":null,"abstract":"Wearable hydrogen (H₂) sensing with fast response and excellent flexibility is highly desired for those workers who are exposed to flammable and explosive H₂, however, it needs further exploring. Here, wearable H₂ sensing with fast response at room temperature (~25 °C) has been developed by integrating palladium hexagonal nanoparticles/carbon nanotubes/cellulose nanocrystals over filter paper (P-Pd HNPs/CNTs/CNCs). By optimizing the wet-chemical approach, Pd HNPs (edge length, ~7.33<!-- --> <!-- -->nm) have been synthesized via the simultaneous reduction of CNCs and ethanol, resulting in a random dispersion around the surface of cross-linked CNTs. Beneficially, P-Pd HNPs/CNTs/CNCs shows fast response/recovery time (3<!-- --> <!-- -->s/3<!-- --> <!-- -->s) to 0.5<!-- --> <!-- -->v/v% H₂ and can detect 50 ppm H₂. Additionally, it exhibits excellent wearable sensing durability, including the resistance to freezing, pressing and peeling off the sensing materials. Theoretically, such fast-response H₂ sensing can be interpreted that H₂ is adsorbed over Pd to form PdHx intermediates, and the “bridging behavior” between CNCs and CNTs facilitates the H₂ diffusion and promotes electron hopping and tunneling. Potentially, this “bridging behavior” that contributes to fast-response H₂ sensing might be applied to other sensing materials for wearable gas detection.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"78 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Colorimetric-Electrochemical Methods for Selective Identification and Quantification of Scopolamine in Forensic Analysis Using Screen-Printed Graphite Electrodes and Dragendorff Reagent","authors":"Larissa M.A. Melo, Elena Bernalte, Ana C.M. Oliveira, Robert D. Crapnell, Rodrigo M. Verly, Rodrigo A.A. Munoz, Wallans T.P. dos Santos, Craig E. Banks","doi":"10.1016/j.snb.2024.137131","DOIUrl":"https://doi.org/10.1016/j.snb.2024.137131","url":null,"abstract":"In forensic investigations, the detection of Scopolamine, popularly known as Burundanga or Devil’s Breath, is of significant interest due to its potential involvement in cases of attempted murder or suicide. Currently, no efficient screening methods exist for Scopolamine detection in such forensic contexts. This study presents a novel method combining screen-printed graphite electrodes (SPGE) with square-wave voltammetry (electrochemical step) and Dragendorff reagent (colorimetric step) to detect Scopolamine in drinks (gin, tonic water, whisky, and energy drinks) and biological samples (urine, saliva, and vitreous humor). The method provides two distinct analytical responses: a visible color change (from orange to yellow) via the colorimetric reaction, and the electrochemical behavior of Scopolamine in both anodic and cathodic scans, ensuring robust and accurate identification. For the first time, the electrochemical behavior of both redox processes of Scopolamine is investigated. The proposed method demonstrated a wide linear range (0.025 and 0.225<!-- --> <!-- -->mg<!-- --> <!-- -->mL⁻¹ for the oxidation and 0.025 to 0.175<!-- --> <!-- -->mg<!-- --> <!-- -->mL⁻¹ for the reduction process) with a low limit of detection of 5.0<!-- --> <!-- -->μg<!-- --> <!-- -->mL⁻¹, making it suitable for forensic applications. Stability of the electrochemical response was studied with SPGE showing relative standard deviations (RSD) of less than 3% for <em>E</em>_<em>p</em> and <em>I</em>_<em>p</em> across multiple electrodes (N = 3). Interference studies confirmed the method's high selectivity for Scopolamine detection. Additionally, Scopolamine was successfully identified in both beverage and biological samples with recoveries near 100%, indicating the absence of matrix effects. The methodology using both electrochemical with a colorimetric approach presents a promising, rapid, and selective screening method for Scopolamine detection in forensic scenarios.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"11 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}