Sensors and Actuators B: Chemical最新文献

Sub-Doppler trace-gas photoacoustic spectroscopy 亚多普勒痕量气体光声光谱

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-10-01 DOI: 10.1016/j.snb.2026.140993

Jacopo Pelini,Stefano Dello Russo,Chenghong Zhang,Zhen Wang,Iacopo Galli,Pablo Cancio Pastor,Maria Concetta Canino,Alberto Roncaglia,Naota Akikusa,Xunzhou Xiao,Wei Ren,Paolo De Natale,Mario Siciliani de Cumis,Simone Borri

引用次数: 0

Machine learning-assisted limonene sensor based on hierarchical WO3 polyhedrons for non-Invasive diagnosis of liver disease via exhaled breath analysis 基于分层WO3多面体的机器学习辅助柠檬烯传感器在呼气分析中无创诊断肝病中的应用

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-06-16 DOI: 10.1016/j.snb.2026.140370

Yanan Fu, Ping Wang, Lijie Zhao, Dongcai Su, Lili Sui, Bing Zhao, Yan Jiang, Haixia Yu

{"title":"Machine learning-assisted limonene sensor based on hierarchical WO3 polyhedrons for non-Invasive diagnosis of liver disease via exhaled breath analysis","authors":"Yanan Fu, Ping Wang, Lijie Zhao, Dongcai Su, Lili Sui, Bing Zhao, Yan Jiang, Haixia Yu","doi":"10.1016/j.snb.2026.140370","DOIUrl":"https://doi.org/10.1016/j.snb.2026.140370","url":null,"abstract":"Non-invasive early detection of liver disease via breath analysis is a long-sought, yet challenging, target in sensor research. High humidity, complex chemical composition, and substantial inter-individual differences in exhaled breath significantly complicate the breath analysis for liver disease diagnosis. In this work, hierarchical WO<ce:inf loc=\"post\">3</ce:inf> polyhedrons were successfully prepared via a simple hydrothermal method. The as-prepared WO<ce:inf loc=\"post\">3</ce:inf> material showed the highest ever-reported response (268.2) to 10 ppm limonene (a critical biomarker for liver diseases) at 100°C, with a remarkable detection limit of 10 ppb. Various characterization techniques, including O<ce:inf loc=\"post\">2</ce:inf>-TPD, XPS, and gas chromatography-mass spectrometry (GC-MS), were employed to thoroughly analyze the sensing mechanism of WO<ce:inf loc=\"post\">3</ce:inf> toward limonene. Based on a comprehensive understanding of the entire sensing process, a robust few samples with high feature dimensionality orient machine learning algorithm was used to assist the sensor in decoding response features specific to limonene from complex breath analysis response singles, thereby achieving 99.9% correct classification rate for distinguishing exhaled signals from liver disease patients and healthy controls in 200 independent cross-validation tests. This work presents the first successful integration of machine learning with a highly sensitive WO<ce:inf loc=\"post\">3</ce:inf>-based limonene sensor, offering significant potential for non-invasive, real-time, and cost-effective early detection of liver diseases.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"24 1","pages":"140370"},"PeriodicalIF":8.4,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148290983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen vacancy-enriched MoO3 hierarchical tubules via loofah sponge template for boosting high response triethylamine detection 利用丝瓜海绵模板制备富氧空度MoO3分层小管，提高三乙胺的高响应检测

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-06-16 DOI: 10.1016/j.snb.2026.140367

Shi-Kai Shen, Yu-Ying Xin, Hui-Ye Jiang, Ya-Nan Li, Zhao-Peng Deng, Ying-Ming Xu, Xin-Lei Cui, Li-Hua Huo, Shan Gao

{"title":"Oxygen vacancy-enriched MoO3 hierarchical tubules via loofah sponge template for boosting high response triethylamine detection","authors":"Shi-Kai Shen, Yu-Ying Xin, Hui-Ye Jiang, Ya-Nan Li, Zhao-Peng Deng, Ying-Ming Xu, Xin-Lei Cui, Li-Hua Huo, Shan Gao","doi":"10.1016/j.snb.2026.140367","DOIUrl":"https://doi.org/10.1016/j.snb.2026.140367","url":null,"abstract":"Monitoring triethylamine (TEA), a highly carcinogenic and explosive substance, is crucial for contemporary environmental governance and personal safety. However, achieving high-response TEA detection with MoO<ce:inf loc=\"post\">3</ce:inf>-based sensors at low power consumption remains a challenge. Herein, biomimetic MoO<ce:inf loc=\"post\">3</ce:inf> hierarchical tubules synthesized via a biotemplate technology significantly enhance low temperature sensing performance for TEA vapor. After calcining a loofah sponge template simply impregnated with molybdate in air atmosphere at 500 °C, biomimetic MoO<ce:inf loc=\"post\">3</ce:inf> hierarchical tubules cross-linked by small sized nanosheets were successfully fabricated. The material exhibits a three-level mesoporous distribution, a relatively small bandgap, large specific surface area and abundant oxygen vacancy defects. Gas sensing tests reveal that at the optimized operating temperature of 133 °C, the MoO<ce:inf loc=\"post\">3</ce:inf>-LS-5 sensor exhibits a high response value of 567 to 50 ppm TEA vapor and short response time of 5 s to 10 ppm TEA vapor. It also exhibits a low detection limit of 10 ppb, good selectivity and moisture resistance. Especially, the MoO<ce:inf loc=\"post\">3</ce:inf>-LS-5 sensor can satisfactorily monitor the spoilage process of shrimp, demonstrating promising application potential in environmental and food safety monitoring. In addition, the detailed analysis of the response mechanism indicates that this outstanding sensitivity stems from the synergistic effect of the microstructural advantages of the tubular MoO<ce:inf loc=\"post\">3</ce:inf>-LS-5 biomimetic material, the high concentration of active adsorbed oxygen and the catalytic action of surface vacant Mo<ce:sup loc=\"post\">6+</ce:sup> Lewis acid sites.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"15 1","pages":"140367"},"PeriodicalIF":8.4,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148290984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Performance Xylene Sensor Based on Cu-doped SnO2 Nanoarchitectures with Low-Temperature Operation and Excellent Selectivity 低温、高选择性cu掺杂SnO2纳米结构的高性能二甲苯传感器

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-17 DOI: 10.1016/j.snb.2026.140197

Shu He , Chunjie Wang , Luohuo Ding , Chengyun Lao , Zhuoqing Zhao , Liangyu Zhai , Yu Huang , Gangqiang Zhu , Meijuan Chen

{"title":"High-Performance Xylene Sensor Based on Cu-doped SnO2 Nanoarchitectures with Low-Temperature Operation and Excellent Selectivity","authors":"Shu He , Chunjie Wang , Luohuo Ding , Chengyun Lao , Zhuoqing Zhao , Liangyu Zhai , Yu Huang , Gangqiang Zhu , Meijuan Chen","doi":"10.1016/j.snb.2026.140197","DOIUrl":"10.1016/j.snb.2026.140197","url":null,"abstract":"<div><div>As a predominant volatile organic carcinogenic compound in indoor environments, xylene poses significant challenges for chemiresistive gas sensors due to its high chemical stability, including the requirement for high operating temperatures and limited selectivity. Herein, Cu-doped SnO<sub>2</sub> porous nanoflowers were synthesized using a hydrothermal method for xylene detection. Notably, the test results showed that SnO<sub>2</sub> with a Cu doping ratio of 1.5wt% (1.5Cu/SnO<sub>2</sub>) exhibited the optimal sensing performance (S = 27.13 towards 100 ppm xylene at 100<!--> <sup>o</sup>C). The sensitivity was 3.1 times that of pure SnO<sub>2</sub>, and it also represented the lowest temperature required to achieve this response value in existing reports. Furthermore, 1.5Cu/SnO<sub>2</sub> achieved superior selectivity toward xylene, with a response value that is 411% of the second highest responding gas (formaldehyde), and it could also maintain specific adsorption capabilities for xylene even in complex VOC atmospheres. The developed gas sensor also demonstrated excellent repeatability, long-term stability, and resistance to humidity interference. Structural characterization and theoretical calculations indicated that copper doping enhanced both the specific surface area and oxygen vacancy concentration of Cu/SnO<sub>2</sub>. Furthermore, the surface Cu sites facilitated xylene adsorption and methyl group activation, facilitated charge transfer during the reaction process, and promoted xylene desorption from the surface prior to complete oxidation. Consequently, the synergistic effect substantially improved the sensitivity and selectivity for low-temperatures xylene detection.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"464 ","pages":"Article 140197"},"PeriodicalIF":3.7,"publicationDate":"2026-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147998200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A variation in thiophene-extended nucleosides as microenvironment-sensitive fluorescent probes 噻吩延伸核苷作为微环境敏感荧光探针的变异

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-09 DOI: 10.1016/j.snb.2026.139639

Tomotaka Kumagai , Tatum Melati Andini , Hiroshi Sugiyama , Daron M. Standley , Soyoung Park

{"title":"A variation in thiophene-extended nucleosides as microenvironment-sensitive fluorescent probes","authors":"Tomotaka Kumagai , Tatum Melati Andini , Hiroshi Sugiyama , Daron M. Standley , Soyoung Park","doi":"10.1016/j.snb.2026.139639","DOIUrl":"10.1016/j.snb.2026.139639","url":null,"abstract":"<div><div>Fluorescence-coded nucleoside analogues, in which photophysical properties are rationally encoded into the nucleobase scaffold, offer powerful tools for probing nucleic acid structure and function in complex biological systems. However, the development of structurally tunable, environment-sensitive fluorescent nucleosides that retain high biocompatibility and versatility remains limited. To address this, we present a tunable platform of <sup><strong>Thex</strong></sup><strong>T</strong>-based fluorescent nucleosides, developed by introducing electronically distinct donor groups. This modular design enables fine-tuning of their photophysical behavior, resulting in donor-dependent variations in solvatochromism, viscosity sensitivity, and quantum yield—hallmarks of fluorescent molecular rotors. <sup><strong>aniThex</strong></sup><strong>T</strong> and <sup><strong>mopThex</strong></sup><strong>T</strong> were synthesized and site-selectively incorporated into DNA without disrupting base-pairing fidelity or duplex stability, preserving their rotor-like characteristics within oligonucleotide strands. When embedded into the G4-forming aptamer AS1411, these probes exhibited fluorescence turn-on effects upon G4 folding and selective nucleolin binding. This enhancement was further evaluated using gel-shift assays, and live-cell imaging, where <sup><strong>Thex</strong></sup><strong>T</strong> derivatives-labeled AS1411 enabled targeted visualization of nucleolin-overexpressing MDA-MB-231 cancer cells. Collectively, this work showcases our <sup><strong>Thex</strong></sup><strong>T</strong> derivatives as versatile fluorogenic probes with broad applicability in nucleic acid structure analysis, biomolecular interaction mapping, and functional cellular imaging.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139639"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening the split DNA inhibitors of the CRISPR/Cas12a to enable ultrasensitively detect single-base mutations 筛选CRISPR/Cas12a的分裂DNA抑制剂，以便超灵敏地检测单碱基突变

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-09 DOI: 10.1016/j.snb.2026.139638

Qiuting Zhang , Shuang Zhao , Jiashuo Xu , Gang Cao , Ziying Zhang , Jing Xu , Ran Luo , Cheng Zhu , Shenghui Li , Xiaoqun Gong , Dianming Zhou

{"title":"Screening the split DNA inhibitors of the CRISPR/Cas12a to enable ultrasensitively detect single-base mutations","authors":"Qiuting Zhang , Shuang Zhao , Jiashuo Xu , Gang Cao , Ziying Zhang , Jing Xu , Ran Luo , Cheng Zhu , Shenghui Li , Xiaoqun Gong , Dianming Zhou","doi":"10.1016/j.snb.2026.139638","DOIUrl":"10.1016/j.snb.2026.139638","url":null,"abstract":"<div><div>Accurate and sensitive identification of single-base mutations (SBM) assumes paramount importance in tumor diagnosis, prognosis, and personalized therapy. Nevertheless, reliable detection of SBM at low abundance remains a formidable challenge. Interestingly, we discovered that when a single-stranded DNA (ssDNA) target was split between 8th and 9th bases, the resulting fragments can act as inhibitors that significantly influence the activation of Cas12a, providing a new handle for SBM analysis. Herein, we developed a new method combining the ligase reaction and the designed Cas12a system for sensitively detecting SBM in glioma-associated Isocitrate Dehydrogenase 1 (<em>IDH-1</em>) enzyme mRNA. Meanwhile, the reverse fluorescence-enhanced paper-based strips (rFEPs) were introduced for the visual and fluorescent detection platform. The limit of detection reached remarkable aM level and the allele frequency achieved at 0.1 %. Moreover, the platform also enabled portable detection of SBM in simulated mutant-type (MT) mRNA cell samples. This study provided a novel method for developing powerful diagnostic tools for tumor detection and precision medicine.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139638"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen vacancy engineering in hollow double-shelled Zn2SnO4/SnO2 heterostructure for efficient n-butanol detection 空心双壳Zn2SnO4/SnO2异质结构中的氧空位工程用于正丁醇的高效检测

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-09 DOI: 10.1016/j.snb.2026.139642

Guoli Yang , Haipeng Wu , Min Li , Jin Kang , Yi Liu , Yongqiang Chen , Liyong Du

{"title":"Oxygen vacancy engineering in hollow double-shelled Zn2SnO4/SnO2 heterostructure for efficient n-butanol detection","authors":"Guoli Yang , Haipeng Wu , Min Li , Jin Kang , Yi Liu , Yongqiang Chen , Liyong Du","doi":"10.1016/j.snb.2026.139642","DOIUrl":"10.1016/j.snb.2026.139642","url":null,"abstract":"<div><div>Hollow multi-shelled heterostructures have garnered significant research interest due to their unique microstructural and electronic characteristics. In this study, hollow double-shelled Zn<sub>2</sub>SnO<sub>4</sub> (ZTO)/SnO<sub>2</sub> heterostructures are successfully synthesized via a facile self-sacrificing template method. By varying the heating rate during annealing, the concentration of oxygen vacancies in the material is effectively modulated. Multiple characterization techniques, including X-ray photoelectron spectroscopy (XPS), photoluminescence (PL) and electron paramagnetic resonance (EPR), confirm that the sample annealed at a heating rate of 10 °C/min exhibits the highest concentration of oxygen vacancies. This sample also demonstrates good gas-sensing performance. Specifically, the ZTO/SnO<sub>2</sub>-3 sensor exhibits a high response value of 62.3 toward 100 ppm n-butanol at a working temperature of 180°C, along with a rapid response time (10 s), good selectivity, repeatability and long-term stability. The enhanced gas-sensing properties are attributed to the synergistic effects of the hollow double-shelled structure, heterojunction formation and oxygen vacancies, which collectively increase the number of active sites on the material surface and facilitate charge carrier transport. Furthermore, in situ Fourier transform infrared spectroscopy (FTIR) is utilized to probe the dynamic process of the gas-sensing reaction between the material and n-butanol. The study also provides deeper insights into the underlying mechanism and establishes a valuable experimental and theoretical foundation for future research.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139642"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CRISPR/Cas12a–personal glucose meter biosensor for rapid and quantitative detection of Burkholderia pseudomallei DNA CRISPR/ cas12a -个人血糖仪生物传感器快速定量检测假马氏伯克氏菌DNA

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-13 DOI: 10.1016/j.snb.2026.139622

Nini Luo , Yuping Ruan , Jian Luo , Shen Tian , Dai Kuang , Nan Zhang , Xiaoxia Xie , Zhangmeng Liu , Xiaobing Wang , Juan Yao , Huangxian Ju , Qianfeng Xia

{"title":"A CRISPR/Cas12a–personal glucose meter biosensor for rapid and quantitative detection of Burkholderia pseudomallei DNA","authors":"Nini Luo , Yuping Ruan , Jian Luo , Shen Tian , Dai Kuang , Nan Zhang , Xiaoxia Xie , Zhangmeng Liu , Xiaobing Wang , Juan Yao , Huangxian Ju , Qianfeng Xia","doi":"10.1016/j.snb.2026.139622","DOIUrl":"10.1016/j.snb.2026.139622","url":null,"abstract":"<div><div>Melioidosis, caused by <em>Burkholderia pseudomallei (B. pseudomallei)</em>, is a fatal tropical infectious disease whose diagnosis is hindered by the lack of rapid and accessible detection methods. Here, we present a novel CRISPR/Cas12a-based biosensing platform integrated with a personal glucose meter (PGM) for rapid, quantitative, and amplification-free detection of <em>B. pseudomallei</em> DNA. In this system, target DNA activates Cas12a trans-cleavage, releasing invertase from an invertase-Linker-biotin probe. Subsequent magnetic separation removes the uncleaved complexes, and the invertase remaining in the solution catalyzes the conversion of sucrose to glucose, which is quantitatively measured by the PGM. The assay is completed within 50 min and achieves a detection limit of 0.1 pM with a linear dynamic range from 1 pM to 10 nM. The biosensor exhibits excellent specificity against single- and multi-base mismatched sequences, outstanding precision (CV < 10 %), and strong correlation with qPCR results in clinical samples (recovery: 98 % - 113 %). The reagents remain stable over four weeks of storage (CV = 4.4 %), confirming the robustness of the system. This amplification-free platform, independent of specialized laboratory instrumentation, represents an important step toward accessible molecular diagnostics, demonstrating the potential as a rapid, low-cost, and user-friendly solution for early melioidosis detection and potentially other infectious diseases. From a sensor-engineering standpoint, the CRISPR/Cas12a–PGM architecture constitutes a generalizable chemical/biosensing platform that can be readily reprogrammed for different nucleic-acid targets by simply switching the crRNA.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139622"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146198837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic amplification via CHA–HCR for electrochemical and colourimetric dual-mode detection of carotid atherosclerosis biomarker CHA-HCR协同扩增用于颈动脉粥样硬化生物标志物的电化学和比色双模检测

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-14 DOI: 10.1016/j.snb.2026.139650

Jiayi Chen , Guolin Yuan , Hui Shang , Xiandong Li , Xiaopeng Guo , Yanan Hu , Dewen Zhu , Fei Xie , Longchuan Xie , Yijun Tang , Chunyan Peng

{"title":"Synergistic amplification via CHA–HCR for electrochemical and colourimetric dual-mode detection of carotid atherosclerosis biomarker","authors":"Jiayi Chen , Guolin Yuan , Hui Shang , Xiandong Li , Xiaopeng Guo , Yanan Hu , Dewen Zhu , Fei Xie , Longchuan Xie , Yijun Tang , Chunyan Peng","doi":"10.1016/j.snb.2026.139650","DOIUrl":"10.1016/j.snb.2026.139650","url":null,"abstract":"<div><div>Long non-coding RNA <em>SOX2-OT</em> (lncRNA <em>SOX2-OT</em>) has demonstrated notable potential for its clinical application as a potential biomarker of carotid atherosclerosis. However, accurate lncRNA-based carotid atherosclerosis diagnosis is hindered by the lack of ultrasensitive, simple, and highly specific detection methods. To tackle these challenges, we introduced an innovative strategy by integrating catalytic hairpin assembly (CHA) and hybridisation chain reaction (HCR) into a cascade signal amplification strategy to detect lncRNA <em>SOX2-OT</em> and enable precise carotid atherosclerosis diagnosis. CHA enables specific target recognition and initial signal amplification, whereas subsequent cascaded HCR generates long double-stranded DNA scaffolds with multiple G-quadruplexes. Simultaneously, methylene blue interacts with DNA structure, generating a potent electrochemical signal. Furthermore, the formation of peroxidase-mimicking hemin/G-quadruplex can catalyse 3,3’,5,5’-tetramethylbenzidine oxidation, enabling visual detection of the target. The biosensor exhibited a low limit of detection of 10 aM and successfully detected lncRNA <em>SOX2-OT</em> in serum samples. This CHA–HCR biosensor, with its enzyme-free, label-free, and highly sensitive nature, holds notable potential for clinical applications, particularly in assessing the severity of carotid atherosclerosis. This study introduces an ultrasensitive and highly specific method for lncRNA detection and offers a new analytical perspective and technical support for the diagnosis and molecular assessment of carotid atherosclerosis.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139650"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pd/Pt-decorated poly(tannic acid) nanospheres as a multifunctional label for ultrasensitive lateral flow immunoassay of methamphetamine Pd/ pt修饰的聚单宁酸纳米微球作为甲基苯丙胺超灵敏侧流免疫分析的多功能标记

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-16 DOI: 10.1016/j.snb.2026.139652

Min Li , Xianqing Tang , Yanjie Li , Zongcheng Zhang , Weijiang Zhou , Jadera Talap , Jian Sun , Ji Zhou

{"title":"Pd/Pt-decorated poly(tannic acid) nanospheres as a multifunctional label for ultrasensitive lateral flow immunoassay of methamphetamine","authors":"Min Li , Xianqing Tang , Yanjie Li , Zongcheng Zhang , Weijiang Zhou , Jadera Talap , Jian Sun , Ji Zhou","doi":"10.1016/j.snb.2026.139652","DOIUrl":"10.1016/j.snb.2026.139652","url":null,"abstract":"<div><div>Methamphetamine (METH) abuse remains a pressing global health threat, driving the demand for on-site, ultra-sensitive detection tools. Herein, we design and fabricate a triple-functional nanozyme through a rational structural design strategy, involving the in situ reduction of Pd/Pt precursors on poly(tannic acid) nanospheres (PTAN). The phenolic-rich surface of PTAN@Pd/Pt allows for one-step, covalent-free antibody immobilization while preserving bioactivity and, concurrently, creates a synergistic catalytic interface. When integrated into a lateral flow immunoassay (LFIA), the platform affords visual limits of detection of 1.0 ng/mL (colorimetric signal) and 0.5 ng/mL (catalytic signal), representing 15- and 30-fold improvements over conventional gold nanoparticle-based LFIAs, and lowers the drug concentration cut-off value (critical value) to 19 ng/mL and 40 ng/mL, respectively. Quantitative analysis showed excellent linearity, and validation in spiked hair, serum, and urine samples yielded high recoveries and low coefficients of variation (CVs). The PTAN@Pd/Pt nanozyme therefore offers a powerful and practical tool for rapid METH screening in clinical diagnostics and forensic science.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139652"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0