Sensors and Actuators B: Chemical最新文献

{"title":"Synergistic effects of Au nanoparticles in SmFeO3-based p-type semiconductors for ethanol and toluene detection","authors":"Tatsuya Joutsuka ,&nbsp;Shinsuke Ishiguro ,&nbsp;Kosei Kuryu ,&nbsp;Masami Mori ,&nbsp;Hiromichi Aono ,&nbsp;Hiroyuki Yamaura ,&nbsp;Yoshiteru Itagaki","doi":"10.1016/j.snb.2025.138142","DOIUrl":"10.1016/j.snb.2025.138142","url":null,"abstract":"<div><div>This study investigates the synergistic effects of integrating gold (Au) nanoparticles into SmFeO₃-based p-type semiconductors for detecting volatile organic compounds (VOCs), specifically ethanol and toluene. The incorporation of Au nanoparticles significantly enhances the sensor's selectivity and sensitivity toward ethanol, primarily due to strong adsorption and catalytic oxidation processes at the Au-SmFeO₃ interface. X-ray photoelectron spectroscopy (XPS) analysis reveals that increasing Au content enhances the excess amount of lattice and adsorbed oxygen species at the SmFeO₃ surface. Scanning electron microscopy (SEM) analysis reveals the uniform distribution of Au nanoparticles on the SmFeO₃ surface. Using the density functional theory (DFT) calculations, the adsorption of ethanol is stronger at the SmFeO₃ surface while that of toluene is slightly weaker at the SmFeO₃ surface than the Au surface. Especially, the Au-SmFeO₃ interface is a strong binding site for ethanol, which can facilitate sensor detection. In addition, migration of O atoms is much more facile at the Au surface, indicating that oxygen species migrate from the Au surface to the three-phase interface to enhance the oxidation of VOCs. These findings underscore the potential of the Au-SmFeO₃ composite as a highly selective material for ethanol detection in VOC sensing applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"442 ","pages":"Article 138142"},"PeriodicalIF":8.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144305191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategic design of self-disassembling Co-PDI fluorescent architecture for dual detection of ascorbic acid and alkaline phosphatase in biological systems","authors":"Chan Yang,&nbsp;Weiyu Qiu,&nbsp;Zihan Yan,&nbsp;Yanru Qin,&nbsp;Yingfang Yuan,&nbsp;Liuting Mo,&nbsp;Weiying Lin","doi":"10.1016/j.snb.2025.138141","DOIUrl":"10.1016/j.snb.2025.138141","url":null,"abstract":"<div><div>Alkaline phosphatase (ALP) serves as a crucial biomarker in clinical diagnostics, where its activity levels directly correlate with various pathological conditions. In this work, we present a rationally designed fluorescence sensing platform based on a cobalt-perylene diimide (Co-PDI) metal-organic coordination complex that exhibits red emission for the simultaneous detection of ascorbic acid (AA) and ALP activity. The sensing mechanism operates through competitive coordination between AA and PDI ligands at Co²⁺ binding sites. This competition triggers the release of PDI molecules, which subsequently form aggregates at high concentrations, leading to fluorescence quenching. Through systematic optimization, the system demonstrates high selectivity and sensitivity for both AA and ALP detection. To enhance practical applicability, we developed a smartphone-integrated detection platform, enabling real-time visual monitoring of analytes. The practical efficacy of this sensing system was validated through extensive testing in complex biological matrices, including cancer cell lysates, liver fibrosis mouse serum, and raw milk samples. The platform successfully detected elevated ALP expression in both liver cancer cells and fibrotic mouse serum. This robust sensing platform offers a promising tool for precise ALP quantification in complex biological environments, potentially facilitating early disease diagnosis and monitoring.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"442 ","pages":"Article 138141"},"PeriodicalIF":8.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144305190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced plasmon resonance through controllable block copolymer self-assembly","authors":"Seungyun Jo ,&nbsp;Jisoo Park ,&nbsp;Seungjae Lee ,&nbsp;Goomin Kwon ,&nbsp;Kahyun Kim ,&nbsp;Mingeun Park ,&nbsp;Hyungju Ahn ,&nbsp;Woo-Dong Jang ,&nbsp;Jeonghun Kim ,&nbsp;Du Yeol Ryu","doi":"10.1016/j.snb.2025.138119","DOIUrl":"10.1016/j.snb.2025.138119","url":null,"abstract":"<div><div>Simple light manipulation in plasmonic systems enables amplification of surface-enhanced Raman scattering (SERS). This study first proposes the fabrication of portable block copolymer (BCP)-based distributed Bragg reflector (DBR) films and their integration into a SERS substrate based on Au nanoparticles (AuNPs), as a novel solution to facilitate scanning of the SERS sensitivity as a function of working wavelength (<em>λ</em>_<em>w</em>). Using a lamella-forming polystyrene-<em>b</em>-poly(2-vinyl pyridine) (PS-<em>b</em>-P2VP) copolymer, a template of defect-free multilayer nanostructures was achieved via thermal annealing under a symmetric confinement. The crosslinking control and incorporation of lactic acid (LA) into the 2VP units produced highly asymmetric giant lamellar structures, which led to BCP-based DBR films with feasible tunability in the reflected wavelength from visible to near-infrared regions. An independently attachable feature of the BCP-based DBR films allowed an immediate modulation of <em>λ</em>_<em>w</em> for the designated SERS sensor. The SERS sensitivity was significantly enhanced (by approximately 99 times) at a specific <em>λ</em>_<em>w</em> = 522 nm, and the integrated sensor had a remarkably low limit of detection (0.2 ppm) for the pesticide, Thiram, on the surface of apples under field conditions. Owing to its exceptional sensitivity and ease of fabrication, the proposed DBR-integrated SERS sensor demonstrates practical potential for food safety, agriculture, and environmental monitoring.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"442 ","pages":"Article 138119"},"PeriodicalIF":8.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144296220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In situ construction of heterojunctions between Co3O4 nanonets and Fe2O3 nanospheres for efficient triethylamine detection","authors":"Huan Liu ,&nbsp;Qingcai Chen ,&nbsp;Tengfei Xu ,&nbsp;Ke Ji ,&nbsp;Bo Wang ,&nbsp;Heng Liu ,&nbsp;Wenhao Liu ,&nbsp;Jingwen Cheng ,&nbsp;Yukun Hu ,&nbsp;Shu Yin ,&nbsp;Chuanyi Wang ,&nbsp;Jincai Zhao","doi":"10.1016/j.snb.2025.138137","DOIUrl":"10.1016/j.snb.2025.138137","url":null,"abstract":"<div><div>Semiconductor metal oxide (MOS) sensors are extensively utilized for toxic gas detection but are often constrained by high operating temperatures, prolonged detection times, and safety risks in flammable environments. Herein, a MOF-derived Fe₂O₃/Co₃O₄ composite with a high specific surface area and abundant oxygen vacancies was synthesized <em>via</em> one-step annealing, achieving <em>in situ</em> p-n heterojunction formation. This innovative structure enabled rapid TEA detection at a low temperature of 110 °C, with a high response of 36.5–50 ppm TEA, which is 4.17 times higher than that of pure Co₃O₄. The heterojunction facilitated efficient charge separation and transport, while oxygen vacancies and the porous structure improved adsorption kinetics. Theoretical calculations further confirm the strong adsorption energy of the Fe₂O₃/Co₃O₄ toward TEA, enabling ultra-fast response and recovery times of 21 s and 33 s, respectively. Compared to the conventional MOS sensors that require high working temperatures, Fe₂O₃/Co₃O₄ sensors exhibit a lower temperature of 110 °C, resulting in reduced power consumption and improved potential for microelectronic integration. This work provides a novel, efficient approach for TEA detection, with potential applications in industrial safety and food quality monitoring.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"442 ","pages":"Article 138137"},"PeriodicalIF":8.0,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of TyG index in the whole blood using mid-infrared fiber evanescent wave spectroscopic sensor","authors":"Yongkun Zhao ,&nbsp;Yinsheng Xu ,&nbsp;Zhifei Yang ,&nbsp;Yantao Xu ,&nbsp;Haitao Guo ,&nbsp;Zheng Zhao ,&nbsp;Tianxiang You ,&nbsp;Mengling Xia ,&nbsp;Minghong Yang ,&nbsp;Xianghua Zhang","doi":"10.1016/j.snb.2025.138138","DOIUrl":"10.1016/j.snb.2025.138138","url":null,"abstract":"<div><div>The triglyceride-glucose (TyG) index has emerged as a reliable surrogate marker for assessing insulin resistance, emphasizing the need for a rapid and precise technique to quantify blood glucose and triglyceride levels. In this study, we have developed a mid-infrared fiber evanescent wave spectroscopic (MIR-FEWS) sensor, tapered from Ge₁₀As₂₀Ga₅Se₆₅ infrared fiber, for the simultaneous analysis of both triglyceride and glucose. This innovative sensor achieves a low limit of detection (LoD) of 1.18 mM for glucose and 0.23 mM for triglycerides, respectively, showcasing its possibility for blood test. Moreover, the sensor exhibits excellent repeatability and stability, validating its applicability for determining the TyG index in whole blood. When applied to measure the TyG index in Sprague-Dawley (SD) rats, the results closely align with those from commercial blood glucose and lipid meters, underscoring its accuracy. The proposed MIR-FEWS sensor has multiple advantages, including multi-component analysis capabilities, high sensitivity, straightforward operation, label-free measurement, and cost-effectiveness, thereby presenting a novel and promising technique for rapid and reliable TyG index detection.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"442 ","pages":"Article 138138"},"PeriodicalIF":8.0,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T-type differential Helmholtz photoacoustic sensor for ultra-sensitive gas detection with noise suppression","authors":"Zhuobei Deng ,&nbsp;Yafei Li ,&nbsp;Yanyu Jiang ,&nbsp;Yanxiong Guan ,&nbsp;Chen Huang ,&nbsp;Yifan Wang ,&nbsp;Shuo Yang ,&nbsp;Wanling Deng ,&nbsp;Junkai Huang ,&nbsp;Chuantao Zheng ,&nbsp;Tuan Guo","doi":"10.1016/j.snb.2025.138140","DOIUrl":"10.1016/j.snb.2025.138140","url":null,"abstract":"<div><div>To achieve ultra-sensitivity gas detection, a photoacoustic (PA) gas sensor system was developed using a multi- pass T-type resonance-enhanced differential Helmholtz (MTRDH) PA cell. The MTRDH PA cell combines the advantages of both the T-type PA cell and the Helmholtz PA cell, increasing the intensity of PA signal, while greatly suppressing the background noise. The structure of the MTRDH PA cell was optimized by comprehensively considering the absolute sound pressure, resonant frequency and the quality factor. The performance of the sensor system was evaluated using a distributed feedback laser with a central wavelength of 1532.68 nm for acetylene (C₂H₂) detection. Two right-angle prisms were installed at both ends of the buffer cavity to form a multi-pass PA cell, achieving 4 reflections of incident light. Compared with the single-pass PA cell, the PA signal of the multi-pass PA cell increased by 3.49 times. The experimental results show that the sensor system has a good linear response to the C₂H₂ gas concentration, achieving a limit of detection of 105 ppb and a normalized noise equivalent absorption coefficient of 1.98 × 10⁻⁹ cm⁻¹ W Hz^−1/2.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"442 ","pages":"Article 138140"},"PeriodicalIF":8.0,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Split activator ligation assay: A CRISPR/Cas12a-enabled biosensor for the genotyping of cancer-associated miRNA single-nucleotide polymorphisms","authors":"Xin Jin ,&nbsp;Shuang Feng ,&nbsp;Wei Lv ,&nbsp;Dong Shen ,&nbsp;Bingzhi Li","doi":"10.1016/j.snb.2025.138134","DOIUrl":"10.1016/j.snb.2025.138134","url":null,"abstract":"<div><div>Although assays for microRNA (miRNA) detection have been largely developed and applied, the identification of single-nucleotide polymorphisms in miRNAs (miR-SNPs), which are emerging cancer biomarkers, remains a significant challenge. The rs11614913 SNP in miR-196a2, resulting in the mutant miR-196a2T variant with a single nucleotide change from the wildtype miR-196a2C, has been linked to cancer diagnosis. In this work, we present the <strong><u>s</u></strong>plit <strong><u>a</u></strong>ctivator <strong><u>l</u></strong>igation <strong><u>a</u></strong>ssay (SALA) for miR-SNP genotyping, marking the first use of CRISPR/Cas12a in this context. In SALA, miR-196a2T facilitates the ligation of split Cas12a activators via SplintR ligase, leading to the full activation of Cas12a and subsequent fluorescence generation. SALA detects miR-SNPs at femtomolar concentrations, allowing for the precise detection of SNPs when the wildtype allele is in over 2000-fold excess. Analyses of clinical samples demonstrate the robustness of SALA in distinguishing cancerous from non-cancerous samples, underscoring its potential utility in cancer diagnostics.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"442 ","pages":"Article 138134"},"PeriodicalIF":8.0,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Noninvasive electrochemical biosensor for rapid detection of Helicobacter pylori in patient saliva","authors":"Stella Schuster da Silva ,&nbsp;Tatiana Lima Valério ,&nbsp;Bruna M. Hryniewicz ,&nbsp;Hussamaldeen Jaradat ,&nbsp;Mel De Souza Wendhausen Araújo ,&nbsp;Carla Adriane Royer ,&nbsp;Beatriz Borba Ortiz ,&nbsp;Andrei Elias Deller ,&nbsp;Leandro Yoshimi Kashiwagui ,&nbsp;Paulo de Souza Fonseca Guimarães ,&nbsp;Jaqueline de Carvalho Oliveira ,&nbsp;Daniela Fiori Gradia ,&nbsp;Olfa Kanoun ,&nbsp;Carolina Camargo de Oliveira ,&nbsp;Marcio Vidotti","doi":"10.1016/j.snb.2025.138136","DOIUrl":"10.1016/j.snb.2025.138136","url":null,"abstract":"<div><div>The carcinogenic bacterium <em>Helicobacter pylori</em> infect over half the global population, with a higher prevalence in low-income countries. It is linked to diseases such as chronic gastritis, peptic ulcers, and gastric cancer, a leading cause of cancer-related deaths worldwide. The gold standard diagnostic method involves histopathological evaluation of gastric biopsy tissue via upper gastrointestinal endoscopy—an invasive, complex, and time-intensive procedure requiring specialized professionals. This study presents a noninvasive alternative: an impedimetric electrochemical biosensor for <em>H. pylori</em> antigen detection in saliva. The biosensor was developed using a screen-printed carbon electrode modified with polypyrrole nanotubes and carboxylated multi-walled carbon nanotubes. Monoclonal antibodies targeting CagA, a cytotoxic effector protein of <em>H. pylori</em>, were immobilized on carbon nanotubes using EDC/NHS chemistry. The platform was characterized via scanning electron microscopy and electrochemical techniques. Electrochemical impedance spectroscopy (EIS) was employed for analyte detection, with an analytical curve constructed in phosphate-buffered saline (PBS) spiked with CagA protein. The biosensor demonstrated a linear detection range of 0.5 pg mL⁻¹ to 3.3 ng mL⁻¹ and a detection limit of 109.9 fg mL⁻¹ . Validation with patient saliva samples, confirmed by histopathology and rapid urease testing, yielded 97 % accuracy, 80 % sensitivity, and 100 % specificity. Results were obtained within 15 min. This EIS-based biosensor offers a sensitive, innovative, and noninvasive platform for early <em>H. pylori</em> diagnosis, which is essential for preventing severe diseases such as gastric cancer.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"442 ","pages":"Article 138136"},"PeriodicalIF":8.0,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein-confinement synergizes with aggregation-induced-emission enhancement strategy for tetracycline detection","authors":"Jiangle Yi ,&nbsp;Yuanling Sun ,&nbsp;Xueying Wang ,&nbsp;Yu Du ,&nbsp;Rui Feng ,&nbsp;Xue Dong ,&nbsp;Xuejing Liu ,&nbsp;Hongmin Ma ,&nbsp;Qin Wei","doi":"10.1016/j.snb.2025.138133","DOIUrl":"10.1016/j.snb.2025.138133","url":null,"abstract":"<div><div>Spatial confinement strategy presents a novel approach to addressing the luminescence quenching of aggregation-induced-emission (AIE) materials in solution. However, traditional synthetic carriers suffer from batch-to-batch variability and poor biocompatibility. A novel aggregation-induced-electrochemiluminescence (AIECL) probe with core-shell structure was developed based on natural nanocavities of ovalbumin (OVA) as a biocompatible carrier through molecular self-assembly. This strategy innovatively integrates protein confinement with AIECL, effectively suppressing the luminescence quenching of tetrakis(4-aminophenyl)ethene (ETTA) in solution and significantly enhancing electrochemiluminescence (ECL) performance. Furthermore, OVA confinement offered multiple advantages: OVA provided dual-confinement through covalent bonds and hydrophobic interactions that enhanced the stability and ECL intensity of OVA/ETTA; OVA improved the aqueous solubility of ETTA while mitigating its cytotoxicity; the mild synthesis conditions without high temperature or pressure significantly reduced energy consumption; OVA provided active sites for aptamer (Apt) conjugation. The introduction of the coreaction promoter further enhanced the ECL responses of the aptasensor, enabling precise detection of tetracycline in the range of 0.1 pM∼1 μM with a detection limit of 42.6 fM. This strategy not only expands the application of food proteins in AIECL sensing but also adheres to Green Chemistry principles and provides new insights into the development of an environmentally-friendly, and low-energy-consumption ECL analysis platform.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"442 ","pages":"Article 138133"},"PeriodicalIF":8.0,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CN- as a cofactor enhancing the laccase-like activity of copper porphyrin metal-organic frameworks facilitates the sensitive cyanide detection","authors":"Xiang Liu ,&nbsp;Haoyu Chen ,&nbsp;Xiaojun Hu ,&nbsp;Jing Liu ,&nbsp;Xiaohua Zhu ,&nbsp;Youyu Zhang ,&nbsp;Meiling Liu","doi":"10.1016/j.snb.2025.138135","DOIUrl":"10.1016/j.snb.2025.138135","url":null,"abstract":"<div><div>Excessive presence of cyanide in various environmental and biofluids poses a significant threat due to its highly toxic nature and potential threat to living organisms. Nevertheless, a multitude of current techniques encounter difficulties in sensitivity and selectivity issues. Inspired by the Cu-N active sites in natural laccase, we propose novel copper-based porphyrin metal-organic frameworks (Cu-TCPP) nanosheets that act as potent laccase mimics, which has been firstly used for the ultra-sensitive cyanide detection. The electron-rich nature, strong reductivity, and Cu-ion coordination ability of cyanide ions (CN^-) specifically enhances the mimic activity of Cu-TCPP. The incorporation of CN^- also increases the Cu⁺/Cu²⁺ ratio and oxygen vacancies, accelerates the interaction between Cu-TCPP and its substrates, enhancing the electron transfer and improving oxygen activation efficiency and catalytic cycling. Notably, the regulatory effect of CN^- is concentration-dependent, enabling sensitive CN^- detection. To address potential interferences from the inherent color and UV absorption of the nanomaterials, a post-reaction centrifugation technique has been introduced, allowing for accurate measurement with improved resolution and sensitivity. The innovative strategy of utilizing a cofactor to regulate the laccase mimics activity provides fresh insights into the design of advanced nanozymes and offers an alternative method for detecting cofactors, facilitating broader applications in various fields.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"442 ","pages":"Article 138135"},"PeriodicalIF":8.0,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}