Sensors and Actuators B: Chemical最新文献

{"title":"Programmable FPGA-based TDLAS-WMS hygrometer (PFTWH) for broad-range water vapour detection: Quantitative assessment of digital lock-in amplifier (DLIA) performance via integrated WMS system (I-WMS)","authors":"Minyoung Choi ,&nbsp;Gyuwon Cho ,&nbsp;Seyeon Hwang ,&nbsp;Jinwoo Lee ,&nbsp;Yangmo Kim ,&nbsp;Jeongmin Kim ,&nbsp;Kyunghoon Kim ,&nbsp;Hongyun So ,&nbsp;Sun Choi","doi":"10.1016/j.snb.2025.138930","DOIUrl":"10.1016/j.snb.2025.138930","url":null,"abstract":"<div><div>This study demonstrates the capability of wavelength modulation spectroscopy (WMS) based on tunable diode laser absorption spectroscopy (TDLAS) using a hand-held field-programmable gate array (FPGA) system. Programmable FPGA-based TDLAS-WMS hygrometer (PFTWH) enables measurement of water vapour concentrations over a broad range (70–5800 ppm) for applications in climate science. This system incorporates a custom-designed digital lock-in amplifier (DLIA), optimized for water vapour detection. For the DLIA, a 100 Hz cut-off frequency and a finite impulse response filter with a windowed-Hamming design and a 200-tap coefficient were used. The PFTWH demonstrated simultaneous processing capability of 1 f and 2 f signals by programming the DLIA on a commercial FPGA board. To quantitatively evaluate DLIA performance, we introduced an integrated WMS system that enables parallel operation of a commercial FPGA-based TDLAS-WMS hygrometer (CFTWH), the PFTWH, and direct TDLAS (dTDLAS). The 1 f and 2 f signals are extracted from both the CFTWH and PFTWH, while the dTDLAS provides reference concentration information for performance assessment. The linearity and accuracy of the designed PFTWH were validated by comparison with the CFTWH. Additionally, long-term measurements of water vapour concentrations across the broad range (70–5800 ppm) were successfully performed with a time resolution of 0.1 s, maintaining stable performance over 1000 s with 30 s averaging. Furthermore, both PFTWH and CFTWH were demonstrated to meet uncertainty, accuracy, and precision requirements for airborne measurement.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138930"},"PeriodicalIF":3.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145326839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flexible SERS substrate based on active nanoparticle enrichment for ultra-trace in situ detection","authors":"Shaoshuai Han ,&nbsp;Yangyang Yu ,&nbsp;Di Lian ,&nbsp;Xin Yang ,&nbsp;Ning Li ,&nbsp;Tangcheng Huang ,&nbsp;Shaojing Duan ,&nbsp;Meijing Du ,&nbsp;Jun Ren ,&nbsp;Zhenlin Wu","doi":"10.1016/j.snb.2025.138945","DOIUrl":"10.1016/j.snb.2025.138945","url":null,"abstract":"<div><div>Surface-enhanced Raman spectroscopy (SERS) has emerged as a powerful technique for applications in food safety testing, environmental monitoring, disease diagnosis, and molecular characterization. However, conventional SERS substrates frequently suffer from limited sensitivity, poor signal uniformity, and dependence on skilled operators. In this study, a flexible and high-performance SERS sensing strategy is proposed, which is based on surface acoustic wave (SAW)-induced clustering of 50 nm gold nanoparticles (AuNPs). When a <span><math><mrow><mn>5</mn><mspace></mspace><mi>μ</mi></mrow></math></span>L AuNP colloidal droplet is dispensed onto the SAW propagation region, acoustic streaming drives the aggregation of nanoparticles. Upon complete evaporation of the droplet, densely packed SERS-active hotspots are formed, enabling strong Raman signal enhancement. By optimizing the thickness of the PDMS flexible film and the input power, the resulting Raman substrate exhibits minimum detectable concentration reaching 10⁻¹³ <!-->M for rhodamine 6G (R6G), 10⁻¹⁰ <!-->M for 4-aminothiophenol (4-ATP), and 10⁻¹¹ <!-->M for crystal violet (CV)—as well as excellent reproducibility. The relative standard deviation (RSD) for CV detection decreased from 45.32% (SAW-free) to 3.59% under SAW-assisted enrichment, confirming the significant improvement in signal uniformity. Finally, the flexible SAW-SERS sensor demonstrates a sensitivity of up to 10⁻⁸ <!-->M for detecting Thiram residues on apple surfaces. This flexible SAW-assisted SERS substrate offers a promising strategy for portable, efficient, and reliable environmental and food safety monitoring.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138945"},"PeriodicalIF":3.7,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145288774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanozyme-driven fenton-RAFT polymerization for highly sensitive PEC–CL dual-mode biosensing of caspase-3","authors":"Huan Wang,&nbsp;Wenyue Zhang,&nbsp;Cuicui Du,&nbsp;Xiaohua Zhang,&nbsp;Jinhua Chen","doi":"10.1016/j.snb.2025.138989","DOIUrl":"10.1016/j.snb.2025.138989","url":null,"abstract":"<div><div>Reversible addition–fragmentation chain-transfer (RAFT) polymerization offers an effective strategy for signal amplification in sensitive biosensing. Herein, we developed a novel photoelectrochemical–colorimetric (PEC–CL) dual-mode biosensing platform for the highly sensitive detection of caspase-3 activity, based on the RAFT polymerization initiated by nanozyme-driven Fenton reaction. Specifically, a magnetic CuFe₂O₄ nanozyme was modified with chain transfer agents (CPAD) to form CPAD-CuFe₂O₄, which was immobilized on the sensing interface via a caspase-3-cleavable DEVD peptide linkage. Upon the introduction of caspase-3, the functionalized CuFe₂O₄ nanozyme was released and catalyzed the Fenton reaction to generate hydroxyl radicals (•OH), thereby initiating RAFT polymerization of Hemin-fixed vinyl monomers (Hemin-VM). The resulting poly(Hemin-VM) (pHemin-VM) was in situ grafted onto the surface of nanozyme, forming a pHemin-VM/CuFe₂O₄ composite that enhanced •OH generation through a positive feedback mechanism, consequently further amplifying the sensing signals. Finally, caspase-3 was sensitively detected through PEC responses arising from polarity switching at the SnS₂/MITO electrode and CL signals generated by the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB). The proposed PEC–CL dual-mode sensing platform exhibited a wide linear range (PEC: 10⁻¹⁶–10⁻⁸ g mL⁻¹, CL: 10⁻¹⁵–10⁻⁸ g mL⁻¹) and low detection limit (5.0 × 10⁻¹⁷ g mL⁻¹ for PEC, and 2.7 × 10⁻¹⁶ g mL⁻¹ for CL) for caspase-3 activity assay. This study introduced a nanozyme-driven Fenton chemistry-initiated RAFT polymerization signal amplification strategy, and expanded its application in biosensing, offering a robust platform for dual-mode detection of disease biomarkers and advancing next-generation biosensor development.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138989"},"PeriodicalIF":3.7,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted conversion from mitochondria to lipid droplets of nitrostyrylindolium by inserting only a styryl group","authors":"Zuo-Hu Chai ,&nbsp;Fujian Qi ,&nbsp;De-Chen Duan ,&nbsp;Yu Zhang ,&nbsp;Neng Wang ,&nbsp;Jian-Jian Kang ,&nbsp;Fang Dai ,&nbsp;Shengxiang Zhang ,&nbsp;Bo Zhou","doi":"10.1016/j.snb.2025.138984","DOIUrl":"10.1016/j.snb.2025.138984","url":null,"abstract":"<div><div>As highly dynamic cellular organelles responsible for the storage of neutral lipids, lipid droplets (LDs) assume crucial roles in maintaining lipid and energy metabolism. Hence, developing fluorescent probes for imaging the dynamics of LDs (generation, fusion, growth, and degradation) can offer valuable insights into numerous pathophysiological processes. This study highlights a targeted conversion strategy for promptly fabricating a novel LD fluorescent probe named <strong>NSSI</strong> by simply inserting a styryl group into nitrostyrylindolium (IR-418), a well-known mitochondria-targeted probe. The insertion of a styryl group enables an increase in the lipophilicity of <strong>NSSI</strong> (Clog<em>P</em> = 6.37) compared with the parent IR-418 (Clog<em>P</em> = 4.10), thereby facilitating its embedding into the waterless core of LDs <em>via</em> hydrophobic interaction, along with an interface-targeting through the electrostatic interaction between its indolium moiety and the negatively charged polar shell of LDs, to achieve its specific targeting towards LDs. By utilizing <strong>NSSI</strong>, we have successfully monitored the morphology and dynamic alterations of LDs in diverse cellular models. We have also observed the LD distribution differences between the infarcted and normal regions in the brain tissues of a photothrombosis stroke mouse model, visualized disrupted hepatic cords and severe steatosis within the liver tissues of an acute liver failure (ALF) mouse model, and witnessed the lipid-rich areas in zebrafish embryos. Overall, these findings signify the potential of <strong>NSSI</strong> as a highly versatile tool for dynamic LD imaging, whether in cellular models, tissue samples, or <em>in vivo</em> scenarios.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138984"},"PeriodicalIF":3.7,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145288923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly responsive detection of triethylamine gas based on Gd-doped MoO3 nanorod sensors: an experimental and first-principles study","authors":"Yonghui Jia","doi":"10.1016/j.snb.2025.138938","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138938","url":null,"abstract":"Triethylamine (TEA) is widely used in industrial production, but it poses a threat to human health. Therefore, it is crucial to develop highly sensitive sensors for TEA. In this paper, Gd-doped MoO₃ nanorod structures with different molar ratios were prepared via a step hydrothermal route by taking advantage of the excellent properties of rare earth elements. After comprehensively characterize, it was confirmed that Gd was successfully doped into the MoO₃ lattice. The sensor based on 3<!-- --> <!-- -->mol% Gd-doped MoO₃ exhibited a larger specific surface area and a higher amount of surface-adsorbed oxygen compared to sensors with other doping ratios. The sensor with this optimal doping ratio has a gas sensing response of about 65.8 for 10 ppm TEA at 150 ℃, which is six times higher than that of the pure MoO₃ nanorods, with a response time of only 16<!-- --> <!-- -->s. In addition, the sensor exhibits excellent selectivity, good reproducibility, and excellent long-term stability. Finally, density-functional theory (DFT) calculations indicate that the gadolinium-doped MoO₃ material significantly improved the sensing performance for TEA. This study provides a valuable reference for the development of metal-semiconductor (MOS) sensors for the detection of TEA.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"102 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145288919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visual fluorescence discovery of the potent photoswitchable hTYR inhibitor to improve treatment strategy for hyperpigmentation","authors":"Dingqiang Fu ,&nbsp;Xilei Ai ,&nbsp;Xiaoyan Li ,&nbsp;Yu Huang ,&nbsp;Bo Liu","doi":"10.1016/j.snb.2025.138988","DOIUrl":"10.1016/j.snb.2025.138988","url":null,"abstract":"<div><div>Hyperpigmentation, which is caused by excess melanin production by melanocytes with human tyrosinase (hTYR) playing a pivotal role, can lead to various dermatological disorders. Therefore, the discovery and utilization of novel human tyrosinase inhibitors represent a promising therapeutic strategy. Herein, a new fluorescent probe <strong>THT-1</strong> was developed for the first time to recognize human tyrosinase instead of mushroom tyrosinase based on the thalidomide fluorescent skeleton. This green probe can not only screen for novel photoswitchable indole-2-ketone-based human tyrosinase inhibitors with better visual sensitivity, but also effectively recognize endogenous tyrosinase at both cellular and zebrafish levels. By meticulously monitoring fluorescence intensity variations, the impact of photoswitchable inhibitors on tyrosinase activity has been tracked, both <em>in vitro</em> and <em>in vivo</em>. These results demonstrate that a multifunctional thalidomide-based probe has been successfully obtained, which facilitates the identification of potent photoswitchable human tyrosinase inhibitors. Moreover, a potent photoswitchable tyrosinase inhibitor <strong>T-1</strong> for reducing melanin levels in A375 cells and zebrafish has been also successfully developed, thus potentially advancing new treatment approaches for hyperpigmentation.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138988"},"PeriodicalIF":3.7,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taguchi optimization of CPTES chemistry for photonic crystal-based biosensors: Detection of anti-SARS-CoV-2 antibodies in human serum","authors":"Agostino Occhicone ,&nbsp;Paola Di Matteo ,&nbsp;Maryam Heidari ,&nbsp;Daniele Chiappetta ,&nbsp;Norbert Danz ,&nbsp;Peter Munzert ,&nbsp;Matteo Allegretti ,&nbsp;Chiara Mandoj ,&nbsp;Alberto Sinibaldi ,&nbsp;Francesco Michelotti","doi":"10.1016/j.snb.2025.138979","DOIUrl":"10.1016/j.snb.2025.138979","url":null,"abstract":"<div><div>Surface functionalization is a pivotal step in the development of biosensors, as it directly influences the immobilization efficiency of biological recognition elements and, consequently, the overall performance of the biosensing platforms. In this study, we employed the Taguchi method to systematically optimize the functionalization parameters of a silicon oxide surface using an organosilane compound. The biosensing platform is based on a one-dimensional photonic crystal structure capable of sustaining Bloch surface waves, which enable highly sensitive optical detection. The use of the Taguchi orthogonal array design allowed us to efficiently explore a limited and representative set of parameter combinations, significantly reducing experimental complexity. Once the optimal functionalization conditions were identified, the biosensors were applied to the analysis of human serum samples (diluted 1:50) collected from both COVID-19 patients and healthy individuals. The system demonstrated the ability to specifically detect IgM antibodies related to SARS-CoV-2 virus, which are typically present in trace amounts. This work presents the first Taguchi-based optimization of a surface functionalization strategy via label-free molecular interactions for biosensing. The approach provides a platform for both optimization and sensitive detection of clinically relevant biomarkers, with clear diagnostic potential.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138979"},"PeriodicalIF":3.7,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly selective room temperature NO2 detection via π-back bonding in CuTPP-functionalized conductive polymer sensors","authors":"Jiyuan Xue ,&nbsp;Bingcheng Shen ,&nbsp;Juan Wang ,&nbsp;Juan Li ,&nbsp;Hongran Zhao ,&nbsp;Tong Zhang","doi":"10.1016/j.snb.2025.138991","DOIUrl":"10.1016/j.snb.2025.138991","url":null,"abstract":"<div><div>Nitrogen dioxide (NO₂), primarily emitted from transportation and industrial activities, poses significant threats to human health and environmental safety. However, reliable, repeatable, highly selective, and sensitive detection of NO₂ at room temperature remains challenging. In biological systems, metalloporphyrins are well known for their essential roles in gas transport, storage, and as catalytic centers in enzymatic redox reactions, demonstrating highly refined molecular recognition capabilities. Inspired by these unique gas-metal porphyrin interactions, we incorporated copper tetraphenylporphyrin (CuTPP) into a conductive polymer composite consisting of polyaniline (PANI) and Poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) to construct a high-performance NO₂ sensor operating at room temperature. Upon exposure to NO₂, Cu²⁺ ions in CuTPP selectively adsorb NO₂ via a non-classical π-back bonding mechanism, which not only ensures high selectivity but also significantly facilitates charge transfer pathways centered around CuTPP, greatly enhancing electron extraction from PANI. For sensor recovery, ultraviolet (UV) was employed to promote the desorption of NO₂. The developed sensor exhibits excellent linearity (R² &gt; 0.97) within an NO₂ concentration range of 0.2–1 ppm, and achieves a theoretical detection limit of approximately 0.4 ppb, suitable for monitoring concentrations of NO₂ in environmental settings. By exploiting the non-classical π-back bonding interaction between CuTPP and NO₂ within a conductive polymer matrix, our approach provides a versatile and low-power strategy for precise NO₂ detection, with potential applications in environmental monitoring and assessment.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138991"},"PeriodicalIF":3.7,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organometallic-derived metal oxide sensors for H₂S detection in an electronic nose for odor abatement assessment","authors":"Justyna Jońca ,&nbsp;Urszula Miller ,&nbsp;Jarosław Szrek ,&nbsp;Artur Muraszkowski ,&nbsp;Adalbert Arsen ,&nbsp;Izabela Sówka ,&nbsp;Katia Fajerwerg ,&nbsp;Myrtil L. Kahn ,&nbsp;Philippe Menini ,&nbsp;Pierre Fau","doi":"10.1016/j.snb.2025.138990","DOIUrl":"10.1016/j.snb.2025.138990","url":null,"abstract":"<div><div>We present the development and application of novel metal oxide gas sensors derived from organometallic and ion-exchange synthesis routes, integrated into an electronic nose platform for the detection of hydrogen sulfide (H₂S) and odorous compounds. The CuO, SnO₂, and WO₃ nanoparticles were characterized using transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). These nanomaterials were deposited as single and double-layer structures forming engineered p–n and n–n heterojunctions. The sensors were evaluated in a wide H₂S concentration range (5 ppb to 50 ppm) under controlled humidity and temperature conditions. Double-layer configurations exhibited significantly enhanced sensitivity, selectivity, and stability compared to commercial MOS sensors. Selected sensors were integrated into a custom-built gas sensing array and used to construct an e-nose system. The system was trained using dynamic olfactometry and applied for odor quantification in real samples from a Municipal Solid Waste Treatment Plant (MSWTP). The e-nose achieved high accuracy in estimating odor concentrations and deodorization efficiency in two full-scale biofilters, outperforming commercial sensor systems. This study presents a complete sensor-to-application workflow, combining nanomaterial design, sensor engineering, statistical modelling, and real-world environmental monitoring. It highlights the potential of organometallic-derived MOS sensors as key components in advanced gas sensing platforms for reliable odor detection in complex industrial environments.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138990"},"PeriodicalIF":3.7,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145288920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing a close link between ferroptosis and drug-induced liver injury with dual-site NIR ratiometric fluorescent probe for mitochondrial peroxynitrite","authors":"Zhengliang Lu ,&nbsp;Xionghao Xu ,&nbsp;Bo Zhao ,&nbsp;Tao Jiang ,&nbsp;Tianxin Liu ,&nbsp;Won Jun Jang ,&nbsp;Juyoung Yoon ,&nbsp;Mei Yan ,&nbsp;Chunhua Fan","doi":"10.1016/j.snb.2025.138985","DOIUrl":"10.1016/j.snb.2025.138985","url":null,"abstract":"<div><div>Drug-induced liver injury (DILI) is a clinically prevalent condition, one of whose pathogenic mechanisms involves overdose or dependent use of drugs such as acetaminophen (APAP). Although ferroptosis-driven by iron metabolism dysregulation and lipid peroxidation accumulation-has been extensively investigated, the theroretical basis for diagnosing DILI remains insufficient. To explore the correlation between DILI and ferroptosis, we developed a dual-reactive-site mitochondria-targeted near-infrared (NIR) ratiometric fluorescent probe (<strong>Mito-Rh-B2</strong>) for detecting ONOO^–. Mito-Rh-B2 not only differentiated between various drug-induced DILI types but also successfully revealed a dose-dependent increase in ONOO^– level in an APAP-induced DILI model. Furthermore, cell experiments demonstrated a strong association between ONOO^–/GSH level and both DILI and ferroptosis. This study provides new insights for future clinical applications related to DILI and ferroptosis.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138985"},"PeriodicalIF":3.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}