Sensors and Actuators B: Chemical最新文献_第2页

Simultaneous and discriminative visualization of endoplasmic reticulum and lysosomes in apoptosis and autophagy using a single fluorescent probe 使用单一荧光探针同时对细胞凋亡和自噬过程中的内质网和溶酶体进行可视化和鉴别性检测

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-20 DOI: 10.1016/j.snb.2024.136981

Rui Yang, Xue Lu, Qinyi Gao, Jiashuo Hu, Xuechen Yao, Fangyuan Yu, Xifeng Yang, Yushen Liu, Shenqiang Wang, Mengmeng Wei

{"title":"Simultaneous and discriminative visualization of endoplasmic reticulum and lysosomes in apoptosis and autophagy using a single fluorescent probe","authors":"Rui Yang, Xue Lu, Qinyi Gao, Jiashuo Hu, Xuechen Yao, Fangyuan Yu, Xifeng Yang, Yushen Liu, Shenqiang Wang, Mengmeng Wei","doi":"10.1016/j.snb.2024.136981","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136981","url":null,"abstract":"The interplay of organelles plays indispensable roles in various biological processes, and their precise interactions contribute to a series of biological functions. Therefore, the exploitation of single probes capable of simultaneously visualizing two organelles is significant yet challenging. In this study, a novel fluorescent probe able to visualize both the endoplasmic reticulum (ER) and lysosomes concurrently was utilized to monitor apoptosis and autophagy. Leveraging the photoinduced electron transfer (PET) mechanisms, the probe (named EL) demonstrated distinct variations in fluorescent intensity within solutions of different pH levels, which enables the differentiation of ER and lysosomes due to their diverse pH values. EL was also effective in monitoring apoptosis with a decrease in fluorescent intensity, and it could visualize the increase of fluorescent intensity in autophagy. Consequently, the discrimination of apoptosis and autophagy processes was achieved. The potential applications of EL in visualizing ER and lysosomes and tracking their dynamic changes during various physiological processes are significant, so this probe would prompt the development of physiology and pathology related to ER and lysosomes.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"19 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D-Printed Microfluidic Device for Cerebrospinal Fluid Diversion: Design, Characterization, and In Vitro Evaluation of an Alternative Shunting Device 用于脑脊液分流的三维打印微流控装置：替代分流装置的设计、表征和体外评估

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-19 DOI: 10.1016/j.snb.2024.136961

Seunghyun Lee, Leandro Castañeyra-Ruiz, Sora Sato, Gio Jison, Brian Hanak, Jenna Ledbetter, Amirhossein Shahriari, Celine Thao-Quyen Tran, Michael Le, Michael Muhonen

{"title":"3D-Printed Microfluidic Device for Cerebrospinal Fluid Diversion: Design, Characterization, and In Vitro Evaluation of an Alternative Shunting Device","authors":"Seunghyun Lee, Leandro Castañeyra-Ruiz, Sora Sato, Gio Jison, Brian Hanak, Jenna Ledbetter, Amirhossein Shahriari, Celine Thao-Quyen Tran, Michael Le, Michael Muhonen","doi":"10.1016/j.snb.2024.136961","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136961","url":null,"abstract":"Hydrocephalus is a neurological disorder characterized by the accumulation of cerebrospinal fluid (CSF) in the brain, resulting in increased intracranial pressure and potentially life-threatening complications. Conventional treatment is CSF shunt implantation but, particularly in pediatric patients, this is fraught with high failure rates of 40% at 2 years and 98% at 10 years. In pursuit of improving current treatment of communicating hydrocephalus, we developed a 3D-printed microfluidic chip that uses flexible material and features a one-way valve to prevent backflow. To evaluate chip performance, we measured flow rate and differential pressure in vitro. We also cultured astrocytes on the device to assess the potential for cellular attachment. The results revealed minimal cellular attachment and absence of obstruction. The ability of this microfluidic chip to effectively regulate CSF flow highlights the potential of microfluidic technology and demonstrates this device’s capacity to serve as a model for the next generation of CSF shunt devices.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"13 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142671053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifouling electrochemical biosensors based on mussel-inspired poly(norepinephrine) and functional peptides for the detection of extracellular signal-regulated kinase 2 in serum 基于贻贝启发的聚（去甲肾上腺素）和功能肽的防污电化学生物传感器用于检测血清中的细胞外信号调节激酶 2

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-19 DOI: 10.1016/j.snb.2024.136964

Hao Zhou, Xiqin Yang, Mingjun Shi, Xijuan Yu, Xiliang Luo

{"title":"Antifouling electrochemical biosensors based on mussel-inspired poly(norepinephrine) and functional peptides for the detection of extracellular signal-regulated kinase 2 in serum","authors":"Hao Zhou, Xiqin Yang, Mingjun Shi, Xijuan Yu, Xiliang Luo","doi":"10.1016/j.snb.2024.136964","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136964","url":null,"abstract":"The adsorption of nonspecific biomolecules on sensing surfaces presents a challenge for accurate and sensitive detection of targets in complex biological samples, which significantly restricts the practical application of biosensors. It is therefore crucial to develop sensing devices with antifouling capabilities. Herein, a low fouling electrochemical biosensing platform has been developed that leverage two kinds of antifouling materials. One material employed is a specially designed functional peptide, while the other is inspired by the adhesive properties of mussels, known as poly(noradrenaline) (PNE). Compared with polydopamine, the PNE is more effective in reducing the nonspecific adsorption in serum samples. The biosensor, which was developed using PNE and peptide, demonstrated exceptional resistance to fouling and a broad liner detection range of 10.0 pg·mL−1 - 10.0 µg·mL−1 for the detection of the target extracellular signal-regulated kinase 2 (ERK2), allowing for accurate measurements over a broad range of concentrations, with a limit of detection of 3.97 pg·mL−1. The antifouling biosensor was able to detect target ERK2 in serum without pretreatment, and with satisfying accuracy comparable to the ELISA kit. This approach for the construction of low fouling biosensors using peptides and PNE can be easily extended to develop other sensing systems for various targets analysis in human body fluids.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"22 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wide range luminescence lifetime-based pH sensing with covalently immobilized multi-protonatable Ru(II) complexes 利用共价固定的多质子 Ru(II) 复合物进行基于寿命的宽范围 pH 值发光传感

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-19 DOI: 10.1016/j.snb.2024.136960

Ya Jie Knöbl, Maximino Bedoya, Alexander Farquharson, Patrick Courtney, Guillermo Orellana

{"title":"Wide range luminescence lifetime-based pH sensing with covalently immobilized multi-protonatable Ru(II) complexes","authors":"Ya Jie Knöbl, Maximino Bedoya, Alexander Farquharson, Patrick Courtney, Guillermo Orellana","doi":"10.1016/j.snb.2024.136960","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136960","url":null,"abstract":"To overcome the limitations of optical pH sensors, the response of which is just ca. one pH unit around the pKa value of the indicator dye and capitalize on the advantages of red-luminescent Ru(II) complexes for optical sensing, multi-pKa members of this family are proposed. Bipyridine ligands bearing protonatable groups (amines and carboxylates) are the basis to design and prepare the indicator dyes. Their photophysical properties and response to pH changes were investigated in the pH 3.5−9.0 range. The complex [Ru(DCB)2DEAMB], where DCB and DEAMB stand for 2,2’-bipyridine-4,4’-dicarboxylate and 4,4’-(N,N-diethylaminomethyl)-2,2’-bipyridine, respectively, shows a reversible monotonic change of its emission wavelength maximum (648 – 634 nm), luminescence intensity (1.40x) and lifetime (335 – 429 ns) in solution with increasing pH. This complex and two similar ones were covalently tethered to commercial TentaGel® M Br polymer microbeads for luminescence phase shift fiberoptic pH measurements. The immobilized [Ru(DCB)2DEAMB] displays the widest pH sensitivity over pH 3.5 to pH 8.5. The stability of this sensor was satisfactory when cycling between pH 3.5 and 8.5 for five days as well as under a three-day cycle between each pH unit. Changes of the buffer type, buffer concentration and osmolarity did not significantly influence the sensor response; however, large variations of dissolved oxygen and, naturally, temperature would require correction by the corresponding sensors. The novel, robust luminescent pH sensor has been tested in phase-sensitive mode for cell cultures monitoring in commercial bioreactors, but its response would also be suitable for in situ monitoring of natural waters.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"13 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142671054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An ultra-low fouling electrochemical mocularly imprinted sensor based on size exclusion effect for highly selective pentachlorophenol detection 基于尺寸排除效应的超低堵塞电化学摩卡印迹传感器，用于高选择性检测五氯苯酚

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-19 DOI: 10.1016/j.snb.2024.136974

Chen Cheng, Xiaoyu Zhu, Xingao Qin, Jinzhu Zhang, Meng Liu, Ying Wang

{"title":"An ultra-low fouling electrochemical mocularly imprinted sensor based on size exclusion effect for highly selective pentachlorophenol detection","authors":"Chen Cheng, Xiaoyu Zhu, Xingao Qin, Jinzhu Zhang, Meng Liu, Ying Wang","doi":"10.1016/j.snb.2024.136974","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136974","url":null,"abstract":"Pentachlorophenol (PCP) is considered as a highly toxic pollutant in water environment. However, detecting low concentrations of PCP precisely hindered by various interferences is still a tough challenge. In this study, an antifouling electrochemical molecularly imprinted (E-MIP) sensor harnessing the size exclusion effect was specifically designed for highly selective detection of PCP in water. The distinctive interaction between the size-selective poly-hydroxyproline helical peptide (PHHP) and poly(o-phenylenediamine) (p(o-PD)) molecularly imprinted films, strongly reduced non-specific adsorption. In the face of high concentrations of organic matters and ions, the reported sensor exhibited robust antifouling capabilities that it preserved 90 % of the initial signal. In addition, the limit of detection (LOD) reached an exceptionally low level of 1.13 nM (S/N = 3), spanning a wide linear range from 1 nM to 10 μM, and the sensor showed high selectivity with an impressive imprinted factor of 12.01 for PCP. Meanwhile, it owned great repeatability and reproducibility, long-term stability for PCP detection within 25 days, and decent reusability (retaining 92.33 % of its senor performance after 5 cycles), which ensured its excellent feasibility for determination of PCP in complex water samples with co-existing interferences due to acceptable recoveries. The development of this strategy provides an innovative platform for PCP quantification in real water samples, underscoring its potential as a key tool in managing PCP at relevant sites.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"99 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct synthesis of composite conductive carbon nanofiber aerogels with continuous internal networks for collaborative physiological signal monitoring under complex environments 直接合成具有连续内部网络的复合导电碳纳米纤维气凝胶，用于复杂环境下的协同生理信号监测

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-19 DOI: 10.1016/j.snb.2024.136975

Shouzhi Yan, Shixin Jin, Xinyan He, Jinhao Xu, Hao Feng, Wenyu Xing, Binjie Xin, Dahua Shou

{"title":"Direct synthesis of composite conductive carbon nanofiber aerogels with continuous internal networks for collaborative physiological signal monitoring under complex environments","authors":"Shouzhi Yan, Shixin Jin, Xinyan He, Jinhao Xu, Hao Feng, Wenyu Xing, Binjie Xin, Dahua Shou","doi":"10.1016/j.snb.2024.136975","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136975","url":null,"abstract":"The emergence of multifunctional wearable flexible sensors has propelled advancements in healthcare and outdoor sports. However, conventional aerogels used for multifunctional sensing often involve complex fabrication processes, lack durability, and struggle to achieve collaborative sensing in complex scenarios, sometimes even lacking conductivity. This paper presents a novel strategy for synthesizing aerogels with interconnected internal networks directly through electrospinning technology, forming a three-dimensional fluffy structure with interlinked nanofibers. Subsequently, ultra-lightweight, highly elastic, and conductive composite carbon nanofiber aerogels (CCNA) were synthesized through high-temperature calcination and in-situ polymerization, suitable for collaborative physiological signal monitoring in complex situations. By controlling the density and phase separation of the charge jet, the three-dimensional fluffy structure of CCNA can be directly formed without strict reliance on external environmental conditions. The combination of CCNA's three-dimensional fluffiness and hydrophobic-hydrophilic properties enables it to operate within an extremely wide range of relative humidity (10% RH - 95% RH), while its internally continuous fiber network structure ensures stable and reliable electrical signal response even after 5000 compression cycles, with a compression response time of only 55ms. The developed flexible wearable electronic device holds promising prospects in healthcare and intelligent sensing applications.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"15 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142673202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly dispersed CeO2 nanocubics supported on hydrogen substituted graphyne sheets for highly NH3 gas sensing detection and humidity independent at room temperature 氢取代石墨烯片上支持的高度分散 CeO2 纳米立方体，用于室温下高度 NH3 气体传感检测且不受湿度影响

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-18 DOI: 10.1016/j.snb.2024.136972

Chuantao Zhang, Lingmin Yu, Senlin Li, Lei Cao, Ning Nan, Rushun Xue, Man Gong, Yaxuan Zhang, Hao Zhang, Xuefeng Xiao, Shanglin Yang, Xinhui Fan, Peichang Shi

{"title":"Highly dispersed CeO2 nanocubics supported on hydrogen substituted graphyne sheets for highly NH3 gas sensing detection and humidity independent at room temperature","authors":"Chuantao Zhang, Lingmin Yu, Senlin Li, Lei Cao, Ning Nan, Rushun Xue, Man Gong, Yaxuan Zhang, Hao Zhang, Xuefeng Xiao, Shanglin Yang, Xinhui Fan, Peichang Shi","doi":"10.1016/j.snb.2024.136972","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136972","url":null,"abstract":"NH3 is a fairly important raw material in a number of fields but harmful to the human. Currently, hydrogen substituted graphyne (HsGY) shows fast response to NH3 gas based on our previous report. However, it still suffers from low response values and humidity interference. CeO2 has the advantage of moisture resistance as NH3 gas sensor but prone to agglomeration, whereas the alkyne bond of HsGY can anchor CeO2 to form a discretely distributed CeO2. Therefore, highly dispersed CeO2 nanocubics anchoring on HsGY sheets are synthesized through hydrothermal method. The gas sensing characteristics show that the gas sensing response value to 80 ppm NH3 is increased by 3 times compared to pure HsGY. Furthermore, they present relatively stable NH3 gas response under relative humidity variation (20–80 %). In situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) is used to reveal the gas sensing mechanism. Energy bands are analyzed using ultraviolet photoemission spectroscopy (UPS), revealing the improvement mechanism of NH3 gas-sensitive performance. Density functional theory (DFT) calculations are employed to explain selectivity mechanism to NH3 against other interference gases. Our research provides novel gas sensing materials for the development of high-performance, moisture-resistant room temperature NH3 sensors.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"46 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142671055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Split-type photoelectrochemical immunosensor integrating ZIF-8@Ag NPs-assisted cation-exchange and Fe2O3/ZnIn2S4 nanoarrays for detection of carbohydrate antigen 72–4 集成 ZIF-8@Ag NPs 阳离子交换和 Fe2O3/ZnIn2S4 纳米阵列的分体式光电化学免疫传感器用于检测碳水化合物抗原 72-4

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-18 DOI: 10.1016/j.snb.2024.136967

Shanghua Liu, Feng Jiang, Yueyuan Li, Huijuan Lin, Qing Liu, Shujun Wang, Qin Wei, Yueyun Li

{"title":"Split-type photoelectrochemical immunosensor integrating ZIF-8@Ag NPs-assisted cation-exchange and Fe2O3/ZnIn2S4 nanoarrays for detection of carbohydrate antigen 72–4","authors":"Shanghua Liu, Feng Jiang, Yueyuan Li, Huijuan Lin, Qing Liu, Shujun Wang, Qin Wei, Yueyun Li","doi":"10.1016/j.snb.2024.136967","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136967","url":null,"abstract":"The sensitivity, accuracy, and stability of a photoelectrochemical (PEC) immunosensor directly depends on the type of sensing substrate and the efficiency of the recognition model in immobilizing biological elements within multilayer assemblies. In this work, a split-type PEC immunosensor integrating Ag nanoparticles-modified ZIF-8 (ZIF-8@Ag NPs)-assisted cation-exchange and Fe2O3/ZnIn2S4 nanoarrays (NAs) as well-ordered sensing substrate and photoactive material was developed for the detection of carbohydrate antigen 72–4 (CA72–4), a novel gastric cancer target. With effectively harvesting visible light, the stability signal output and the rapid carrier separation, the Z-Scheme Fe2O3/ZnIn2S4 NAs heterojunction was successfully grown on an indium tin oxide (ITO) electrode as the signal converter and sensing substrate via two-step solvothermal method. The ZIF-8@Ag NPs were employed as the signal label to release silver ions (Ag+), relying on the acidolysis in the 96-well plate. The released Ag+ initiated a cation-exchange (CE) reaction with the Fe2O3/ZnIn2S4 NAs, forming Fe2O3/ZnIn2S4/Ag2S ternary complexes with higher photocurrent density. This resulted in a positive correlation trend between CA72–4 concentration (0.01 U/mL to 100 U/mL), and improved the recognition sensitivity of the sensor to trace target, with the limit of detection is 0.0032 U/mL. This work represents an important advancement in overcoming the limitations of multilayer assembly in constructing immunosensors, providing a valuable reference for trace detection of low-abundance disease-related biomarkers.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"250 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142671052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A polydopamine coated magnetic spiral switchable composite material for photothermal sterilization and dual-color fluorescence detection of food-borne pathogens 用于光热灭菌和食源性病原体双色荧光检测的聚多巴胺涂层磁性螺旋可切换复合材料

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-17 DOI: 10.1016/j.snb.2024.136935

Jiaqi Feng, Jin Li, Xianfeng Lin, Lixin Kang, Nuo Duan, Zhouping Wang, Shijia Wu

{"title":"A polydopamine coated magnetic spiral switchable composite material for photothermal sterilization and dual-color fluorescence detection of food-borne pathogens","authors":"Jiaqi Feng, Jin Li, Xianfeng Lin, Lixin Kang, Nuo Duan, Zhouping Wang, Shijia Wu","doi":"10.1016/j.snb.2024.136935","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136935","url":null,"abstract":"A multifunctional magnetic composite material (MSp@PDA) featuring with helical structure, superparamagnetism, photothermal properties, and fluorescence quenching capability was synthesized through sequential magnetization and in-situ polydopamine coating on the Spirulina surface, then applied for multi-scenario application of pathogens capture, sterilization, and sensitive detection. The pathogens capture and sterilization was investigated by loading bacterial broad-spectrum recognition molecule (MPBA) on MSp@PDA, the capture efficiencies of 91.6% and 94.2% were obtained for Escherichia coli and Staphylococcus aureus in 15 minutes, respectively, with complete photothermal sterilization achieved within 4 minutes. The fluorescence real-time detection of E. coli and S. aureus was realized by connecting dual-color fluorescent carbon dots to MSp@PDA through specific aptamers (dCD-apt-MSp@PDA), the corresponding detection limits were 2 and 3 cfu·mL-1, respectively, with linear ranges of 101~107 cfu·mL-1. The spiked recovery rates of target bacteria in actual samples were 97.4%~101.1% and 98.2%~101.4% for E. coli and S. aureus, respectively. The constructed capture, sterilization, and fluorescence detection methods demonstrated promising application value for on-site monitoring of food-borne pathogens in complex matrices.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"21 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142645903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a chemiluminescent detection method for absolute activity measurement of Taq DNA polymerase through dATP consumption 开发一种化学发光检测方法，通过消耗 dATP 来测量 Taq DNA 聚合酶的绝对活性

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-17 DOI: 10.1016/j.snb.2024.136918

Wei Jing, Xuefeng Wang, Jingjie Jiang, Ping Gui, Xiao Li, Feng Lu, Yanna Lin, Haichao Li, Huancai Yin, Fuqiang Ma

{"title":"Development of a chemiluminescent detection method for absolute activity measurement of Taq DNA polymerase through dATP consumption","authors":"Wei Jing, Xuefeng Wang, Jingjie Jiang, Ping Gui, Xiao Li, Feng Lu, Yanna Lin, Haichao Li, Huancai Yin, Fuqiang Ma","doi":"10.1016/j.snb.2024.136918","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136918","url":null,"abstract":"Being one of the most extensively researched and widely used thermostable DNA polymerases, Taq DNA polymerase derived from Thermus aquaticus has played pivotal roles in numerous molecular biology applications. The accurate and convenient determination of enzymatic activity is a fundamental prerequisite for both academic research and the industrial application of Taq DNA polymerase. To address the limitations of traditional methods for polymerase activity determination, such as low accuracy, complicated operation, and radioactive hazards, we have devised an absolute-quantitation method for Taq DNA polymerase based on chemiluminescence detection of the consumption of dATP (CDC-dATP). The CDC-dATP method offers several advantages, including simplicity suitable for routine laboratory use, high sensitivity (detecting as low as 0.0025 U/μL), excellent reproducibility, a broad linear range for Taq DNA polymerase (0.0025-0.1 U/μL) with a strong linear relationship (R2 > 0.99), and, significantly, absolute activity quantitation without the associated radioactive hazards. We delved into the molecular behavior of Taq DNA polymerase in the CDC-dATP method. Furthermore, we explored the extensive application potential of the CDC-dATP methodology for measuring the activity of various other enzymes involved in dATP/ATP generation or consumption, encompassing other DNA polymerases, reverse transcriptases, RNA polymerases, hydrolases, and protein kinases, covering all seven major categories of enzymes.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"22 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0