Sensors and Actuators B: Chemical最新文献

{"title":"Double enhanced flower-like SERS sensor based on aptamer modification for high specific recognition and sensitive rapidly detection of kanamycin","authors":"Shan Zhang,&nbsp;Fang Mi,&nbsp;Pengfei Geng,&nbsp;Guotong Chen,&nbsp;Xuehui Rao,&nbsp;Ming Guan","doi":"10.1016/j.snb.2025.138899","DOIUrl":"10.1016/j.snb.2025.138899","url":null,"abstract":"<div><div>Chronic intake of animal-derived foods with excessive kanamycin (Kana) residues risks human drug accumulation, posing significant health threats. To mitigate this, a layer of 4-mercaptophenyl-boronic acid (MPBA) was incorporated between the branched Au nanoflowers (NFs) core and the Ag shell to generate an inter-metallic nanogap, resulting in an aptamer (Apt)-functionalized surface-enhanced Raman scattering (SERS) probe (Au NFs-MPBA@Ag-Apt). When combined with MPBA as the Raman probes, exhibits an intense SERS signal at 1060 cm⁻¹ . Concurrently, magnetic nanoparticles modified with flower-like molybdenum disulfide (Fe₃O₄@MoS₂@Ag-Apt) were synthesized. These particles possess a strongly paramagnetic Fe₃O₄ core enveloped by a high-surface-area, flower-like MoS₂ layer, efficiently anchoring Ag nanoparticles (NPs) and Kana-Apt for enhanced target capture. Coupling semiconductor MoS₂ with Ag NPs creates a coupled enhancement: electromagnetic amplification between Ag NPs and at the Ag NPs-MoS₂ interface boosts “hot spots” density. As a semiconductor, MoS₂ facilitates separation of photogenerated electron-hole pairs when interfaced with Ag NPs, driving substantial interfacial electron transfer toward Kana molecules. This substrate achieves a remarkable enhancement factor (EF) of 8.1 × 10⁹. This integrated SERS sensor, using two types of probes at the same time, enables highly specific recognition, rapid, sensitive quantification of Kana. The detection range was 1 × 10¹ ∼ 1 × 10⁶ pg/mL, and the limit of detection (LOD) was 0.23 pg/mL. Compared to conventional methods, this biosensor exhibits superior detection breadth and sensitivity. Applied to milk analysis, recoveries were 93.0 ∼ 103 %. Its portability aligns with point-of-care testing (POCT) needs, offering a viable platform for food safety monitoring.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"447 ","pages":"Article 138899"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A microfluidic device integrating magnetic stirring with tangential flow for high-efficiency isolation of small extracellular vesicles","authors":"Hao Zhong ,&nbsp;Zeyin Mao ,&nbsp;Xuwen Li ,&nbsp;Anni Deng ,&nbsp;Yihao Zhu ,&nbsp;Shihong Wang ,&nbsp;Leyang Huang ,&nbsp;Jianxin Zhao ,&nbsp;Wenqi Lv ,&nbsp;Yixuan Shi ,&nbsp;Qin Huang ,&nbsp;Yabo Zhai ,&nbsp;Chenzhou Hui ,&nbsp;Xianbo Luo ,&nbsp;Li Ma ,&nbsp;Rongxin Fu ,&nbsp;Xiongjun Ye ,&nbsp;Guoliang Huang","doi":"10.1016/j.snb.2025.138898","DOIUrl":"10.1016/j.snb.2025.138898","url":null,"abstract":"<div><div>Small extracellular vesicles (sEVs), which are ubiquitously present in bodily fluids, hold significant promise as biomarkers for disease diagnosis and targeted therapy. However, their isolation remains challenging due to their small size (typically &lt; 200 nm) and the complexity of biological fluids. This study presents a novel microfluidic strategy integrating Magnetic Stirring with Tangential Flow Filtration (MS-TFF). The automated, portable device (palm-sized, 12.5 cm × 11.1 cm × 6.25 cm) features user-friendly operation: inserting the chip into a slot and closing the lid initiates automated sEV extraction. The system processes 10 mL of morning urine or cell culture supernatant within ∼40 min. Compared to ultracentrifugation (UC), MS-TFF reduces processing time from ∼4 h to ∼40 min while achieving a 6-fold higher sEV yield. Clinical validation using urine from 16 subjects (6 healthy/10 prostate cancer patients) enabled quantitative *KLK3/PCA3* RNA biomarker detection, achieving AUC = 0.93 for cancer diagnosis, confirming its diagnostic potential.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"447 ","pages":"Article 138898"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ni(OH)2/MXene-decorated plasmonic interface with dual signal enhancement for sensitive PSA immunoassay in serum","authors":"Yanpei Xu ,&nbsp;Silong Li ,&nbsp;Shuxian Gao ,&nbsp;Fudong Jia ,&nbsp;Liang Guo ,&nbsp;Jiangrong Hou ,&nbsp;Shaowei Ma ,&nbsp;Heya Zheng ,&nbsp;Chuxiao Sun ,&nbsp;Qi Wang","doi":"10.1016/j.snb.2025.138797","DOIUrl":"10.1016/j.snb.2025.138797","url":null,"abstract":"<div><div>Prostate-specific antigen (PSA) detection is vital for early prostate cancer diagnosis, yet conventional methods suffer from sensitivity and label-dependent limitations. This study presents a surface plasmon resonance (SPR) biosensor enhanced by Ni(OH)<span><math><msub><mrow></mrow><mrow><mn>2</mn></mrow></msub></math></span>/Ti<span><math><msub><mrow></mrow><mrow><mn>3</mn></mrow></msub></math></span>C<span><math><msub><mrow></mrow><mrow><mn>2</mn></mrow></msub></math></span>T<span><math><msub><mrow></mrow><mrow><mi>x</mi></mrow></msub></math></span> MXene nanocomposites (NTC) for sensitive detection of total PSA (tPSA) in serum. The NTC architecture synergizes carboxyl-functionalized MXene, amplifying localized electric fields and antibody-binding sites, with Ni(OH)<span><math><msub><mrow></mrow><mrow><mn>2</mn></mrow></msub></math></span> nanoflowers that prevent MXene stacking and enhance refractive index (RI) sensitivity. Optimized via finite element modeling (50-nm Au film, 72.4 ° incident angle), the Au-NTC architecture achieved a 39.34% improvement in RI sensitivity (3064.86 nm/RIU) compared to pristine Au. Through the dual signal amplification mechanism of synergistic enhancement by nanomaterials and sandwich method with gold nanoparticles, enabled detection of tPSA in 20% serum across a wide dynamic range (0.2–160 ng/mL), fully covering the clinical risk threshold (4–10 ng/mL). The platform demonstrated an ultralow limit of detection (LOD) of 0.0361 ng/mL (0.5157 pM) and high specificity against interferons. Kinetic analysis revealed strong antigen–antibody affinity (equilibrium dissociation constant <span><math><mrow><msub><mrow><mi>K</mi></mrow><mrow><mtext>D</mtext></mrow></msub><mo>=</mo><mn>4</mn><mo>.</mo><mn>26</mn><mo>×</mo><mn>1</mn><msup><mrow><mn>0</mn></mrow><mrow><mo>−</mo><mn>10</mn></mrow></msup></mrow></math></span> M), while batch-producible sensor chips and integrated microfluidics underscored practical applicability. This work advances label-free SPR biosensing for precision prostate cancer diagnostics, offering significant potential in point-of-care testing and early intervention strategies.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"447 ","pages":"Article 138797"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ chemical conversion approach for growth of magnesium-aluminium-layered double hydroxide thin films: Room temperature NO2 sensing","authors":"Shweta V. Talekar ,&nbsp;Prashant D. Sawant ,&nbsp;Shraddha A. Pawar ,&nbsp;Shashikant P. Patole ,&nbsp;Mayura J. Medhekar ,&nbsp;Ganesh L. Khande ,&nbsp;Sayali S. Kulkarni ,&nbsp;Hemraj M. Yadav ,&nbsp;Jayavant L. Gunjakar","doi":"10.1016/j.snb.2025.138890","DOIUrl":"10.1016/j.snb.2025.138890","url":null,"abstract":"<div><div>The direct deposition of layered double hydroxide on a substrate is highly desirable for a gas sensor. In this work, we demonstrate the direct deposition of magnesium-aluminum-layered double hydroxide (MA-LDH) thin film (TF) by chemical conversion of magnesium oxide (MgO) TF. The chemical conversion of MgO TFs produces well-crystalline, porous microsheets composed of an interconnected nanoparticle network of MA-LDH, which provides an enhanced surface area with a highly accessible framework, making MA-LDH TFs promising candidates for efficient gas sensor electrodes. Furthermore, the resulting MA-LDH TFs are tested as resistive sensor electrodes to detect different oxidizing (SO₂, Cl₂, and NO₂) and reducing (LPG, CO, CO₂, H₂, H₂S, and NH₃) gases. The MA-LDH TF exhibited excellent selectivity towards NO₂ gas with a maximum response of 54 % at room temperature (300 ± 2 K) compared to pristine MgO TFs (14 %) at 473 K for 100 ppm. Also, it exhibits a quick response time of 10 s, a detection limit of 0.02 ppm, and prolonged stability. Of prime interest is that the MA-LDH sensor displayed high tolerance to humidity conditions. The excellent NO₂ sensing performance of the MA-LDH TFs is attributed to the expanded surface area, which is composed of porous microsheets formed by interconnected nanoparticles network. The direct deposition of an interconnected microsheet network provides abundant active adsorption sites, thereby enhancing gas sensing efficiency.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"447 ","pages":"Article 138890"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-cost CMOS-based luminescence lifetime imaging with oxygen, temperature and pH sensors","authors":"Gauthier Rousseau ,&nbsp;Marianne Pons ,&nbsp;Hessel Adelerhof ,&nbsp;Nathan Pellerin ,&nbsp;Mart Giesbergen ,&nbsp;Bastien Carde ,&nbsp;Martin Wolff ,&nbsp;Koen Blanckaert ,&nbsp;Sergey M. Borisov ,&nbsp;Benoit Fond","doi":"10.1016/j.snb.2025.138849","DOIUrl":"10.1016/j.snb.2025.138849","url":null,"abstract":"<div><div>Luminescence imaging enables spatiotemporal visualization of physical and chemical quantities, yet widespread adoption has been hindered by expensive, specialized instrumentation. Here, we present a compact platform that employs a consumer CMOS camera for versatile luminescence imaging across biomedical research, geosciences, and aerospace engineering applications. Our approach exploits the on-chip charge accumulation capability to perform multiple exposures in one frame, thereby combining the robustness of lifetime-based measurements with the superior signal quality of time-integrated detection. We demonstrate platform versatility through three applications: (i) oxygen mapping using phosphorescent platinum(II) benzoporphyrin optodes; (ii) the first demonstration of temperature mapping in fluid using thermally-activated delayed fluorescence (TADF) nanoparticles in a laser plane, achieving ±1 °C (1<span><math><mi>σ</mi></math></span>) precision for single-shot single-pixel at a <span><math><mrow><mn>500</mn><mspace></mspace><mtext>µm</mtext></mrow></math></span> spatial resolution and 7 Hz temporal resolution; and (iii) pH mapping through dual lifetime referencing using a combination of a fluorescent aza-BODIPY indicator and phosphorescent Egyptian blue reference material. With total costs of approximately €2,000 for the LED configuration and €4,000 for the laser configuration, our platform reduces instrumentation barriers while maintaining precision and sampling rates comparable to specialized systems. We provide open-source Python and Arduino hardware control code (Clicc - Cumulative Lifetime Imaging with CMOS-Camera) to ensure reproducibility and encourage adoption.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"447 ","pages":"Article 138849"},"PeriodicalIF":3.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145195161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of lung cancer aldehydes biomarker by Al2O3/Ag/ZIF-8 SERS quasi cavity with co-confinement effect","authors":"Lingmeng Fang ,&nbsp;Yunhai Zhang ,&nbsp;Hao Li ,&nbsp;Jing Yu ,&nbsp;Maosen Yang","doi":"10.1016/j.snb.2025.138878","DOIUrl":"10.1016/j.snb.2025.138878","url":null,"abstract":"<div><div>The utilization of surface-enhanced Raman scattering (SERS) technology for monitoring aldehydes in human exhaled gas represents a burgeoning method for early screening of lung cancer; however, it currently faces several challenges: firstly, the limited capacity of SERS substrates to capture aldehyde molecules, and secondly, the low degree of co-localization between aldehyde molecules and hotspot spatial distribution. To address these issues, this study proposes an Al₂O₃/Ag/ZIF-8 SERS quasi-cavity structure, which enables the dual regulation of intramolecular hotspots, the capture of gaseous analytes, and the micro-localized co-localization of \"hotspots-analytes.\" Leveraging this structure, we achieved sensitive detection of ethyl benzaldehyde molecules over a concentration range of 0.2 ppb-20 ppm, and preliminarily explored its specific recognition of aldehyde biomarkers in human exhaled gas. This research provides a versatile SERS detection strategy for gaseous molecules, offering significant reference value for the accurate detection of various cancer-associated gaseous biomarkers in human exhalations.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"447 ","pages":"Article 138878"},"PeriodicalIF":3.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chain-based precision amplification biosensor for microRNA detection based on dCas13a system and iridium complex","authors":"Yiming Zhang ,&nbsp;Zhi Chen ,&nbsp;Songrui Wei ,&nbsp;Yujun Zhang ,&nbsp;Yingxia Liu ,&nbsp;Hui Li ,&nbsp;Jing Wang ,&nbsp;Qi Gao ,&nbsp;Defa Li ,&nbsp;Han Zhang ,&nbsp;Zhongjian Xie","doi":"10.1016/j.snb.2025.138881","DOIUrl":"10.1016/j.snb.2025.138881","url":null,"abstract":"<div><div>Considering the close association between the abnormal expression of multiple microRNAs (miRNAs) and various diseases, multi-channel detection of miRNAs holds significant importance for disease diagnosis. However, traditional miRNA detection methods fall short of simultaneously achieving the required specificity, sensitivity, and multi-channel capability. To address this challenge, we developed a chain-based precision amplification photoelectrochemical biosensor for the simultaneous detection of multiple miRNAs, based on dCas13a system and iridium complex. The precise recognition of miRNA is achieved by the dCas13a-crRNA complex and then converted into a significant photocurrent response from iridium nanocomposite attached to a long double-stranded nucleic acid chain, thereby enabling signal amplification, namely chain-based precision amplification. The dCas13a-crRNA complex is modified onto a multi-channel electrode to simultaneously recognize and bind multiple target miRNAs. The iridium complex is modified on the surface of energy-level-matched quantum dots to suppress carrier recombination, and Au nanoparticles are further modified on the surface of the quantum dots, thereby constructing a nanocomposite for efficient photoelectric conversion. A double-stranded sequence is constructed at the 3′ end of the miRNA to increase the attachment sites for the nanocomposite. The biosensor demonstrates exceptional specificity and sensitivity, capable of distinguishing single-base differences with a detection limit as low as the aM level, benefiting from the chain-based precision amplification. Furthermore, clinical sample testing underscores the practical applicability of the biosensor in clinical diagnostics. This multi-channel chain-based precision amplification biosensor offers a highly valuable and innovative approach for the simultaneous detection of multiple miRNAs, with considerable potential for clinical diagnostic applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"447 ","pages":"Article 138881"},"PeriodicalIF":3.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated intelligent sensing system with WO3/CNTs sensor for on-site gas detection","authors":"Mingyang Gao ,&nbsp;Wen Zhang ,&nbsp;Jun Guo ,&nbsp;Licun Fu","doi":"10.1016/j.snb.2025.138886","DOIUrl":"10.1016/j.snb.2025.138886","url":null,"abstract":"<div><div>Gas sensors integrated with intelligent systems are of great importance for real-time environmental monitoring and personal exposure assessment. In particular, nitrogen dioxide (NO₂), a toxic byproduct of combustion and industrial processes, poses severe risks to human health even at trace levels, highlighting the urgent need for sensitive and portable monitoring technologies. Here, we present a tungsten trioxide (WO₃)/carbon nanotubes (CNTs)-based flexible sensor coupled with an Integrated Intelligent Sensing System (IISS) capable of on-site NO₂ detection, signal processing, and wireless mobile display. The optimized WO₃/CNTs sensor exhibits outstanding sensing performance, delivering a high response of 425.1 % toward 1 ppm NO₂. Distinctively, by integrating the sensor into the IISS platform, real-time NO₂ concentrations can be acquired, processed, and displayed on a mobile device, enabling direct and user-friendly exposure monitoring. These results highlight the novelty of combining a high-performance WO₃/CNTs sensor with a miniaturized IISS, offering a practical strategy for intelligent, wearable, and portable gas detection applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"447 ","pages":"Article 138886"},"PeriodicalIF":3.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SpyTag/SpyCatcher-based self-assembly multivalent anti-idiotypic nanobody mediated environmentally friendly lateral flow immunoassay for mycotoxin zearalenone","authors":"Yuanzhen Guo ,&nbsp;Chong Cai ,&nbsp;Xinyu Xu ,&nbsp;Xiao Wu ,&nbsp;Minwen Sun ,&nbsp;Tailong Wei ,&nbsp;Yujie Shi ,&nbsp;Yubin Liu ,&nbsp;Zhui Tu ,&nbsp;Yanping Li ,&nbsp;Qinghua He","doi":"10.1016/j.snb.2025.138885","DOIUrl":"10.1016/j.snb.2025.138885","url":null,"abstract":"<div><div>Anti-idiotypic nanobodies (AIdnbs) are attractive as biosynthetic surrogate antigens due to their batch consistency, high tolerance and eco-friendly production. However, monomeric AIdnbs suffer from low immobilization efficiency and weak signal response on nitrocellulose filter (NC) membranes in lateral flow immunoassay (LFIA). To address this, we developed a self-assembly multivalent AIdnb system using the zearalenone (ZEN)-specific AIdnb Z6 as a model. We fused SpyTag or SpyCatcher to Z6 via flexible (GS) or semi-rigid (EK) linkers, then assembled bivalent Z6 through SpyTag/SpyCatcher and heptavalent Z6 via SpyTagged IMX313 scaffolds. EK-linked Z6-fusions consistently exhibited superior activity compared with GS-linked fusions due to the superior conformation for antibody binding of EK-linked variants. Adsorption kinetics and LFIA detection demonstrated the superior NC membranes’ immobilization efficiency and analytical performance of multivalent Z6 to monovalent Z6. The EK-linked bivalent Z6 demonstrated optimal performance in time-resolved microspheres-based LFIA, achieving a detection limit of 32.44 pg/mL for ZEN in 15 min, which is 4.7-fold lower than monovalent formats, along with a 4.5-fold broader detection range. Notably, this detection sensitivity surpassed conventional chemically synthesized antigens, achieving a 2.5-fold improvement. Furthermore, Maize, wheat and oat were tested as real samples, detection limits were 1.31–1.94 µg/kg for the bivalent Z6-based LFIA, and 1.62–2.12 µg/kg for heptavalent Z6-based LFIA. This study establishes a generalizable strategy for engineering high-performance multivalent biosynthetic antigens, enabling sensitive, eco-friendly LFIA for small-molecule detection.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"447 ","pages":"Article 138885"},"PeriodicalIF":3.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A prolong glow-type chemiluminescence platform for sensitive detection and photothermal inactivation of Escherichia coli","authors":"Xinghui Yu ,&nbsp;Wenyang Shi ,&nbsp;Junyan Hou ,&nbsp;Kairui Zhai ,&nbsp;Yang Xu ,&nbsp;Jie Zhao ,&nbsp;Xiaodong Lin","doi":"10.1016/j.snb.2025.138888","DOIUrl":"10.1016/j.snb.2025.138888","url":null,"abstract":"<div><div><em>Escherichia coli</em> (<em>E. coli</em>) is a leading cause of hospital-acquired infections, especially in abdominal and urinary tract sites, posing serious risks for patients’ post-kidney transplantation. Rapid and precise detection of <em>E. coli</em> is critical to prevent complications such as bloodstream infections, urinary tract infections, and infectious shock. Here, we present a prolong glow-type chemiluminescence (CL)-based bacterial metabolism platform that achieves both sensitive detection and effective photothermal inactivation of <em>E. coli</em> based on multifunctional nanomaterials. This platform leverages the metabolic activity of <em>E. coli</em> to trigger a CL signal without the need for external excitation sources, facilitating low-cost and highly sensitive detection with a limit of detection as low as 1 CFU/mL. Fe₃O₄ NPs were functionalized with <em>E. coil</em>-specific antibodies, allowing for precise pathogen capture and magnetic separation, ensuring robust sample preparation. After detection, near-infrared (NIR) laser irradiation enabled the Cu_2-XSe NPs to generate localized heat, effectively inactivating <em>E. coli</em> cells with an antibacterial efficiency of up to 99.18%. This multifunctional platform integrates rapid detection and sterilization, demonstrating significant potential for clinical applications in infection management, especially in post-transplant care.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"447 ","pages":"Article 138888"},"PeriodicalIF":3.7,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}