Sensors and Actuators B: Chemical最新文献_第10页

Synthesis of Cu(II)-loaded polydopamine to construct fluorescence sensor for detection of cTnI from myocardial infarction

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-20 DOI: 10.1016/j.snb.2025.137649

Linling Zhong , Fanpeng Gong , Jing Li , Guizhu Liu , Xin Wen , Fan Yu , Changjun Hou , Sen Yang , Fangyong Zhu , Xianfeng Wang

{"title":"Synthesis of Cu(II)-loaded polydopamine to construct fluorescence sensor for detection of cTnI from myocardial infarction","authors":"Linling Zhong , Fanpeng Gong , Jing Li , Guizhu Liu , Xin Wen , Fan Yu , Changjun Hou , Sen Yang , Fangyong Zhu , Xianfeng Wang","doi":"10.1016/j.snb.2025.137649","DOIUrl":"10.1016/j.snb.2025.137649","url":null,"abstract":"<div><div>High-performance detection of cTnI possesses great significance for diagnosis of myocardial infarction. In this work, two kinds of lower-dimensional Cu(II)-loaded polydopamines were synthesized via in-situ polymerization of dopamine for the first time using Cu-pBDC MOFs and Cu-aBDC MOFs as templates. The obtained Cu-ppDA nanosheets and Cu-apDA nanoribbons maintain the original structure of MOFs and exhibit the advantages of large specific surface area and metal coordination effect, which efficiently enhances the adsorption of single-stranded aptamer DNA probe via π–π stacking. Energy transfer from FAM-Apt probe to Cu(II)-loaded pDA occurred, resulting in the fluoresce quenching. The inference of target cTnI assembles with FAM-Apt probe and the releases from FAM-Apt/Cu-ppDA or FAM-Apt/Cu-apDA systems, leading to the fluorescence recovery. This approach enables a simple, rapid, sensitive, and specific detection method for cTnI. By contrast, FAM-Apt/Cu-ppDA system shows a lower LOD than that of FAM-Apt/Cu-apDA. Furthermore, the successful implementation of these two fluorescent sensors for the detection of cTnI in serum samples obtained from mice with myocardial infarction has been demonstrated. The outcomes of this detection aligned with those achieved through the ELISA method, suggesting their promising potential for practical application in early screening for myocardial infarction.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137649"},"PeriodicalIF":8.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reasonable design of aggregation-induced emission luminogens for rapid detection of cardiac troponin I by lateral flow immunoassay

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-19 DOI: 10.1016/j.snb.2025.137635

Panpan Sun , Qian Li , Ningshuang Gao , Mingyue Luo , Wenzhuo Chang , Baodui Wang , Xiaoquan Lu , Zhonghua Xue

{"title":"Reasonable design of aggregation-induced emission luminogens for rapid detection of cardiac troponin I by lateral flow immunoassay","authors":"Panpan Sun , Qian Li , Ningshuang Gao , Mingyue Luo , Wenzhuo Chang , Baodui Wang , Xiaoquan Lu , Zhonghua Xue","doi":"10.1016/j.snb.2025.137635","DOIUrl":"10.1016/j.snb.2025.137635","url":null,"abstract":"<div><div>Aggregation-induced emission luminogens (AIEgens)-loaded nanoparticles (NPs) with unique optical properties have recently emerged as valuable tools in lateral flow immunoassays (LFIA). To further enhance the sensitivity and reliability of LFIA, there is an ongoing demand for fluorescent NPs exhibiting higher brightness. Herein, we developed an ultrabright AIEgens NPs-based LFIA for rapid and quantitative detection of the acute myocardial infarction biomarker, cardiac troponin I (cTnI). We introduced two <em>para</em>-CF<sub>3</sub>-substituted benzenes (<em>p</em>-CF<sub>3</sub>-Ph) groups into the traditional AIE structure to form (<em>E</em>)-4-(4-(bis(4′-(trifluoromethyl)-[1,1′-biphenyl]-4-yl)amino)styryl)-1-octadecylpyridin-1-ium (CF<sub>3</sub>-TPA). The lipophilicity-improved CF<sub>3</sub>-TPA was encapsulated into the hydrophobic cavities of polystyrene microspheres (PS), resulting in AIE<sub>CF3</sub>NPs. Gratifyingly, the quantum yield (QY) of AIE<sub>CF3</sub>NPs conjugated with antibodies (AIE<sub>CF3</sub>NPs-mAb1) remained at 47.24 %, basically consistent with 48.68 % of AIE<sub>CF3</sub>NPs, which guarantees the sensitivity of the strip. Additionally, the Stokes shift of AIE<sub>CF3</sub>NPs reached 125 nm, effectively minimizing significant background fluorescence in the visible region. AIE<sub>CF3</sub>NPs-labeled LFIA showed high sensitivity for cTnI detection in the range of 1–100 ng/mL, with a limit of detection (LOD) of 0.0897 ng/mL and a visible LOD of 10 ng/mL within 10 minutes, demonstrating high specificity and accuracy. Overall, AIE<sub>CF3</sub>NPs has great potential as an effective tool for disease diagnosis in emergency situations.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"436 ","pages":"Article 137635"},"PeriodicalIF":8.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coumarin-based fluorescent probe for hypochlorite detection and imaging of acute kidney injury

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-19 DOI: 10.1016/j.snb.2025.137645

Ying-Ying Kong , Bo Liu , Yu-Hang Zhang , Di Han , Hong-Kun Yang , Jun-Tang Lin , Hong-Cheng Xia

{"title":"Coumarin-based fluorescent probe for hypochlorite detection and imaging of acute kidney injury","authors":"Ying-Ying Kong , Bo Liu , Yu-Hang Zhang , Di Han , Hong-Kun Yang , Jun-Tang Lin , Hong-Cheng Xia","doi":"10.1016/j.snb.2025.137645","DOIUrl":"10.1016/j.snb.2025.137645","url":null,"abstract":"<div><div>Acute kidney injury (AKI) has attracted attention as a frequent clinical disease, and the occurrence of AKI can lead to the decline of kidney function and even endanger life. Realizing early diagnosis of AKI can effectively control and treat the condition. Current clinical diagnostic methods cannot meet the accurate diagnosis of early acute kidney injury. Oxidative stress has been proved to be a causative factor of AKI. Therefore, we constructed a fluorescent probe based on coumarin fluorophore for the detection of hypochlorite (HClO) produced during oxidative stress to realize early diagnosis of AKI. The structure of the probe is simple and easy to synthesize, and it has the ability of colorimetric and fluorescence recognition for HClO. The probe responds to HClO as fast as 3 seconds and shows good selectivity and sensitivity to HClO with a detection limit of 15.6 nM. The probe is also well used for imaging exogenous and endogenous HClO in cells. It also can be successfully used for imaging and diagnosis of cisplatin-induced acute kidney injury.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137645"},"PeriodicalIF":8.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hierarchical flower-like Cu-doped SnO2/Ag2S heterojunctions decorated with Ag for excellent sensing performance toward n-butanol at low operating temperatures

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-19 DOI: 10.1016/j.snb.2025.137638

Shixin Huang , Wei Liu , Hailong Lin , Zhicheng Wen , Chunjin Hang , Rong An , Yongfeng Li , Yanhong Tian

{"title":"Hierarchical flower-like Cu-doped SnO2/Ag2S heterojunctions decorated with Ag for excellent sensing performance toward n-butanol at low operating temperatures","authors":"Shixin Huang , Wei Liu , Hailong Lin , Zhicheng Wen , Chunjin Hang , Rong An , Yongfeng Li , Yanhong Tian","doi":"10.1016/j.snb.2025.137638","DOIUrl":"10.1016/j.snb.2025.137638","url":null,"abstract":"<div><div>The design of materials for the detection of n-butanol at low operating temperatures is of great significance for the reduction of power consumption and the improvement of the safety of the gas sensor. In this study, the solvothermal method and subsequent Ag-loading treatment were employed to synthesise hierarchical flower-like Cu-doped SnO<sub>2</sub>/Ag<sub>2</sub>S heterojunctions decorated with Ag (CSAA). The morphological and structural characterisation demonstrated that the amount of Cu<sup>2 +</sup> doping and the duration of electroless Ag plating played a pivotal role in the morphological evolution of the SnO<sub>2</sub>-based hierarchical structure. The optimal CSAA composite exhibits the highest response of 1136 and the shortest response time of 6 s to 50 ppm n-butanol at 80°C, accompanied by superior selectivity, repeatability, humidity and long-term stability. The enhanced sensing performances of the CSAA sensor can be mainly ascribed to the hierarchical porous structure, the doping of Cu<sup>2+</sup>, the construction of SnO<sub>2</sub>/Ag<sub>2</sub>S heterostructures and the decoration of Ag nanoparticles (Ag NPs). Furthermore, the density functional theory simulation was employed to investigate the influence of Ag NPs and Ag<sub>2</sub>S on the adsorption properties and electronic behaviour of n-butanol on the surface of SnO<sub>2</sub>. This work proposes a friendly strategy and theoretical support for enhancing the sensing performance of MOS sensors in the practical detection of n-butanol.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137638"},"PeriodicalIF":8.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated platform for rotavirus nucleic acid detection

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-19 DOI: 10.1016/j.snb.2025.137642

E-Bin Gao , Wenxi Lu , Yurun Hu , Qinggang Xu , Zhong Ni , Junhua Wu , Guoping Huang

{"title":"An integrated platform for rotavirus nucleic acid detection","authors":"E-Bin Gao , Wenxi Lu , Yurun Hu , Qinggang Xu , Zhong Ni , Junhua Wu , Guoping Huang","doi":"10.1016/j.snb.2025.137642","DOIUrl":"10.1016/j.snb.2025.137642","url":null,"abstract":"<div><div>Group A rotavirus (RVA) is the main cause of acute gastroenteritis in infants and children with high infectivity, continuously presenting a negative impact on society. Therefore, the rapid and accurate detection of RVA is crucial for the prevention and diagnosis of the disease. Here we establish a modular and visual diagnostic platform for nucleic acid detection of the pathogen by integrating four technologies, asymmetric nucleic acid sequence-based amplification (asymmetric-NASBA), enzyme-free toehold-mediated strand displacement (TMSD), programmable primer exchange reaction (PER) cascades-mediated efficient signal amplification and a lateral flow immunoassay (LFIA), namely an ATPL-based platform. The built-in workflow can isothermally complete a convenient and rapid detection of the target molecules with a minimum concentration of 2.46 copies/mL in about 20 min, and markedly generate a lateral flow line signal for the naked eye. Moreover, a single-base difference can be distinguished from the target sequence, demonstrating high specificity and clinically analytical performance. Compared to the current common detection technology RT-qPCR, this platform provides detection results with high consistency with a kappa value of 0.927. In a word, the established ATPL-based detection platform is fast, highly sensitive and specific with promising accuracy, suggesting wide application potential in the detection of rotavirus or other nucleic acid in the future.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137642"},"PeriodicalIF":8.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

“Superior snowflake” AuPd/Cu2S as substrate and satellite-like Au/CeO2 as signal probe to improve the sensitivity of electrochemical sensor for kanamycin detection

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-19 DOI: 10.1016/j.snb.2025.137636

Jinmin Zhang , Zhiguang Suo , Min Wei , Hailin Shen , Xiaohui Chen , Huafen Wang , Yong Liu , Baoshan He , Huali Jin , Renyong Zhao

{"title":"“Superior snowflake” AuPd/Cu2S as substrate and satellite-like Au/CeO2 as signal probe to improve the sensitivity of electrochemical sensor for kanamycin detection","authors":"Jinmin Zhang , Zhiguang Suo , Min Wei , Hailin Shen , Xiaohui Chen , Huafen Wang , Yong Liu , Baoshan He , Huali Jin , Renyong Zhao","doi":"10.1016/j.snb.2025.137636","DOIUrl":"10.1016/j.snb.2025.137636","url":null,"abstract":"<div><div>Many novel assays have been realized for the detection of antibiotics in food and environment. Low sensitivity and poor stability limit their application in electrochemical biosensors. The snowflake-like AuPd/Cu<sub>2</sub>S used in electrochemical sensor has a large area and high conductivity. Using it as an electrode modification material can effectively increase the working area of the electrode, thus further improving the current response. A more perfect snowflake-like Cu<sub>2</sub>S structure were synthesized after optimized for the substrate. After calculation it was surprisingly found that the two most prominent structures in each branch shape were divided into two parts by using them as the dividing line, which basically satisfied the golden section line ratio. Moreover, the modification of snowflake-like AuPd/Cu<sub>2</sub>S resulted in a significant increase in current by a factor of 1.48 compared to the bare electrode. Meanwhile, satellite-like Au/CeO<sub>2</sub> loaded methylene blue (MB) was also used as a signal probe, which has a high and stable current signal. Based on the above, a kanamycin (Kana) detection method based on AuPd/Cu<sub>2</sub>S as a substrate and satellite-like Au/CeO<sub>2</sub> as a signal probe to improve the sensitivity of electrochemical sensor was designed. Detection of Kana was accomplished by changes in target concentration combined with Exonuclease III (Exo Ⅲ) assisted double cycling. Under optimal conditions, the sensor had a detection range of 0.001–1000 ng/mL and a detection limit of 0.299 pg/mL. Repeatability, reproducibility, specificity, stability and Kana in real and quality control samples were successfully verified using the sensor with satisfactory results.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137636"},"PeriodicalIF":8.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoelectrochemical biosensor for 5caC-DNA and Exo Ⅲ detection based on Schottky junction integrated with type-I heterojunction of BiOCl/Bi2S3/CS-MXene and hybridization chain reaction

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-19 DOI: 10.1016/j.snb.2025.137647

Huanshun Yin , Yulin Zheng , Chengji Sui , Yunlei Zhou

{"title":"Photoelectrochemical biosensor for 5caC-DNA and Exo Ⅲ detection based on Schottky junction integrated with type-I heterojunction of BiOCl/Bi2S3/CS-MXene and hybridization chain reaction","authors":"Huanshun Yin , Yulin Zheng , Chengji Sui , Yunlei Zhou","doi":"10.1016/j.snb.2025.137647","DOIUrl":"10.1016/j.snb.2025.137647","url":null,"abstract":"<div><div>The 5-carboxylcytosine (5caC), an active DNA demethylation intermediate, is present in many cells and tissues and plays a key role in the regulation of gene expression. 5caC is highly similar in structure to 5-methylcytosine, 5-hydroxymethylcytosine and 5-formylcytosine, but its abundance is extremely low. To explore the biologic function of 5caC, the sensitive detection technique is required. To achieve this goal, a new photoelectrochemical (PEC) biosensor was developed for 5caC-DNA detection using Schottky junction integrated with type-I heterojunction of BiOCl/Bi<sub>2</sub>S<sub>3</sub>/CS-MXene tetany composite as photoactive material and 5caC-hairpin DNA hybridization triggered hybridization chain reaction (HCR) as signal amplification mode. Based on the double heterojunctions, the photoactivity of BiOCl/Bi<sub>2</sub>S<sub>3</sub>/CS-MXene improved greatly, leading to a high photocurrent response, and achieving high detection sensitivity. To perform HCR amplification, an ingenious hairpin DNA containing 5caC was designed to inhibit the cleavage activity of exonuclease III (Exo III). However, after hybridization with <sub>S1</sub>DNA, the unfolded hairpin DNA triggered the excision of double-stranded DNA to release the 5caC-DNA fragment, which further induced the HCR amplification and capture electron donor of methylene blue (MB) onto the electrode surface, causing an increased photocurrent response, and achieving the highly sensitive detection of 5cac-DNA and Exo III. The proposed PEC biosensor showed a detection range of 1 pM - 1 nM and 1–100 U/mL with detection limits of 0.16 pM and 0.34 U/mL (S/N = 3) for 5caC DNA and Exo Ⅲ, respectively. The developed method can also be applied to screen Exo III inhibitor and evaluate the ecotoxic effect of pollutant.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137647"},"PeriodicalIF":8.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amino nitrogen quantum dots hybrid platinum as efficient peroxidase mimics for fluorescent sensing in microchip

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-18 DOI: 10.1016/j.snb.2025.137641

Jiani Yang, Ling Xia, Gongke Li

{"title":"Amino nitrogen quantum dots hybrid platinum as efficient peroxidase mimics for fluorescent sensing in microchip","authors":"Jiani Yang, Ling Xia, Gongke Li","doi":"10.1016/j.snb.2025.137641","DOIUrl":"10.1016/j.snb.2025.137641","url":null,"abstract":"<div><div>Nanocomposites-based nanozyme have been proposed to realize the combination of the respective properties of each component or to achieve cooperatively enhanced catalytic performances, but the systematic catalytic mechanism remains unclear in majority cases. Herein, an entirely new nanocomposite, amino nitrogen quantum dots hybrid platinum (aNDs/Pt hybrid) nanozyme was fabricated to reach a remarkable peroxidase-like activity owing to the synergetic effects between the two components. The roles of the oxygenous groups on the surface of aNDs/Pt hybrid determining their peroxidase-like catalytic activity were evaluated by regulating the functional groups during the synthesis of nitrogen quantum dots. Accordingly, empirical and theoretical evidences were given to identify the catalytic, substrate-binding, and inhibition sites in peroxidase-mimicking catalytic reactions of the aNDs/Pt hybrid. Meanwhile, the catalytic activity of aNDs/Pt hybrid nanozyme shows a strong dependence on the concentration of H<sub>2</sub>O<sub>2</sub>, based on which a highly sensitive fluorescent sensor for glucose detection in a microchip was established by employing aNDs/Pt hybrid as robust peroxidase. It is found that the developed sensor had excellent glucose sensing capability, including two-section broad linear detection ranges (0.1–6.0 μmol/L and 6.0–1000.0 μmol/L), less sample consumption (2.0 μL) and a low detection limit (31.7 nmol/L). Human serum and saliva samples were successfully examined by the as-proposed sensor with satisfactory results, showing the practical applications. This work not only gives a much deeper understanding on cooperatively catalytic mechanism for nanohybrids-based nanozymes, but also facilitate the design and fabrication of other types of target-specific artificial enzymes.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137641"},"PeriodicalIF":8.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated microfluidic device of DNA extraction, recombinase polymerase amplification and micro-capillary electrophoresis for sample-to-answer detection of Salmonella Typhimurium

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-18 DOI: 10.1016/j.snb.2025.137625

Van Dan Nguyen, Gayathri Sureshkumar, Tae Seok Seo

{"title":"Integrated microfluidic device of DNA extraction, recombinase polymerase amplification and micro-capillary electrophoresis for sample-to-answer detection of Salmonella Typhimurium","authors":"Van Dan Nguyen, Gayathri Sureshkumar, Tae Seok Seo","doi":"10.1016/j.snb.2025.137625","DOIUrl":"10.1016/j.snb.2025.137625","url":null,"abstract":"<div><div>Foodborne pathogens pose a significant global health threat, leading to millions of illnesses and deaths annually. Our study introduces an integrated microfluidic chip for rapid molecular diagnostics of foodborne pathogens. The proposed device can perform DNA extraction, recombinase polymerase amplification (RPA), and micro-capillary electrophoresis (µCE) in a sequential manner. With a compact copper-based thermal unit for RPA and precise on-off power supply for the µCE, we could achieve swift detection <em>InvA</em> gene of <em>Salmonella</em> Typhimurium within 75 min. The entire process includes 10 min for sample lysis, 10 min for genomic DNA extraction, 50 min for the RPA reaction, and 5 min for µCE operation and peak assignment. Within 5 min, we were able to detect the presence of the <em>InvA</em> gene of <em>S.</em> Typhimurium (161 bp) with a LOD of 10<sup>3</sup> CFU/mL in both bacterial culture and spiked milk samples. We used a short bracket ladder (121 bp) and a long bracket ladder (203 bp) to accurately assign the target peak in the electropherogram, and the relative migration time of target gene over two bracket ladders was 0.449 ± 0.0094. Notably, our platform is compact, with all components neatly arranged within a 3D printed frame measuring 12 cm (width) × 14 cm (length) × 2.7 cm (height), excluding the in-house detector. This integrated microsystem from the sample injection to the CE analysis presents promising prospects for advanced on-site pathogen detection, and lays the groundwork for future molecular diagnostic platform to combat foodborne illnesses and understanding of pathogen genome and variations.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137625"},"PeriodicalIF":8.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial intelligence-based gastric cancer detection in the gastric submucosal dissection method via hyperspectral imaging

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-18 DOI: 10.1016/j.snb.2025.137630

Inyoung Park , Jin Roh , Dohyeon Son , Choong-Kyun Noh , Jonghee Yoon

{"title":"Artificial intelligence-based gastric cancer detection in the gastric submucosal dissection method via hyperspectral imaging","authors":"Inyoung Park , Jin Roh , Dohyeon Son , Choong-Kyun Noh , Jonghee Yoon","doi":"10.1016/j.snb.2025.137630","DOIUrl":"10.1016/j.snb.2025.137630","url":null,"abstract":"<div><div>Although endoscopy is a standard diagnostic tool for detecting gastric cancer, challenges persist in identifying cancer and assessing tumor extent, particularly in stomachs with atrophy and intestinal metaplasia. To address this issue, we aimed to introduce a novel, compact hyperspectral imaging system with artificial intelligence (AI) that utilizes structured illumination and hyperspectral imaging to diagnose gastric cancer based on intrinsic tissue optical properties. The optical properties of three types of gastric tissue (normal, adenoma, and gastric cancer) obtained from nine patients collected via endoscopic submucosal dissection were analyzed. Our findings reveal that cancer tissue displays unique optical properties, such as low reduced scattering coefficients and distinct reflectance spectral profiles when compared to normal and adenoma tissues. However, it was challenging to diagnose gastric cancer accurately using optical properties with conventional analysis methods due to their heterogeneity. Therefore, we employed a Vision Transformer model with a supervised learning approach to accurately classify tissue types based on intrinsic optical properties. To accurately train the AI model, we devised a novel image processing method to obtain single-pixel level ground-truth labeling data by aligning pathology results and imaging data. The trained AI model successfully demonstrated its ability to diagnose gastric cancer accurately in the nine patients studied. Given its compact design and rapid imaging capabilities, the proposed optical system can be a versatile clinical tool for on-site endoscopic diagnosis and could potentially aid in complete endoscopic tumor removal.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137630"},"PeriodicalIF":8.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0