Sensors and Actuators B: Chemical最新文献

{"title":"Multi-quenching electrochemiluminescence system based on resonance energy transfer from self-enhanced Ce(III, IV)-MOF@Ru to CuO@PDA@AuNPs for ultrasensitive detection of HER2","authors":"Hongying Jia ,&nbsp;Nuo Zhang ,&nbsp;Yuyang Li ,&nbsp;Hongmin Ma ,&nbsp;Dan Wu ,&nbsp;Xuejing Liu ,&nbsp;Huangxian Ju ,&nbsp;Qin Wei","doi":"10.1016/j.snb.2024.136905","DOIUrl":"10.1016/j.snb.2024.136905","url":null,"abstract":"<div><div>This study presents an innovative approach based on electrochemiluminescence resonance energy transfer (ECL-RET) through the introduction of a new pattern donor-acceptor couple. The donor of self-enhanced Ce(III, IV)-MOF@Ru is created by immobilizing Ru(bpy)₃²⁺ on Ce-based metal-organic frameworks (Ce(III, IV)-MOF). The acceptor of CuO@PDA@AuNPs is the CuO nanospheres polydopamine (PDA) framework grafted with gold nanoparticles (AuNPs). An ultrasensitive detection of human epidermal growth factor receptor-2 (HER2) was achieved through the development of a quenched ECL immunosensor. Ce(III, IV)-MOF, a unique 3D infinite extension framework, was recognized for its excellent nanostructure and remarkable capacity to greatly activate tripropylamine (TPrA) and generate abundant radicals. Fortunately, it was simultaneously utilized as a highly effective coreactant accelerator and encapsulation agent, thereby facilitating the immobilization of Ru(bpy)₃²⁺ and the generation of radicals for creating a self-enhanced emitter of Ce(III, IV)-MOF@Ru. Consequently, by effectively reducing the distance of electron transmission and minimizing the loss of energy, Ce(III, IV)-MOF@Ru achieved a significantly high efficiency in ECL. More importantly, CuO@PDA@AuNPs was prepared as a perfect quenching agent. The ultraviolet-visible (UV–vis) spectra of CuO@PDA@AuNPs exhibited partial overlap with ECL spectra of Ce(III, IV)-MOF@Ru, thus efficiently initiating the ECL-RET interaction between the donor and acceptor. With the purpose of demonstrating the superiority of newly obtained self-enhanced nanoemitter and donor-acceptor couple, an ECL immunoassay was proposed for the analysis of HER2 with range of 0.2 fg/mL ∼ 10 ng/mL and the limit of detection of 0.067 fg/mL. Therefore, this method supplies convenient and significant strategy for the clinical analysis.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136905"},"PeriodicalIF":8.0,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142588836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In situ two-photon fluorescence spectroscopy to precisely reveal intracellular lipid droplet microenvironment via a carbonized polymer dot nanosensor","authors":"Zitong Yu ,&nbsp;Xiumian Cao ,&nbsp;Zepeng Huo ,&nbsp;Weiqing Xu ,&nbsp;Shuping Xu","doi":"10.1016/j.snb.2024.136900","DOIUrl":"10.1016/j.snb.2024.136900","url":null,"abstract":"<div><div>Lipid droplets (LDs) are vital dynamic cellular organelles that maintain lipid equilibrium and support biological communication. The polarity of LDs is critical, as their heterogeneity is closely related to pathophysiological developments. To investigate these changes, we developed a polarity-specific two-photon fluorescence (TPF) platform for real-time and precise monitoring of the polarity microenvironment of intracellular LDs with a carbonized polymer dots (CPD) probe. This platform enables the accurate and <em>in situ</em> polarity assessment of a single LD. Using this platform, we identified differences in LD polarity between cancerous and non-cancerous cells, as well as variations in LD polarity during pathophysiological events such as ferroptosis and steatosis. The development of the CPD probe associated with TPF spectroscopy marks a significant advancement in understanding the role of LDs in lipid-associated diseases.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136900"},"PeriodicalIF":8.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142580355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Zn-based metal coordination polymer for ultrafast capture and electrochemical sensing of Hg(Ⅱ)","authors":"Zixuan Chen ,&nbsp;Haiying Lin ,&nbsp;Qingge Feng ,&nbsp;Jiatong Lv ,&nbsp;Lianghong Li ,&nbsp;Jia Lin ,&nbsp;Xianghua Wu ,&nbsp;Lixin Huang ,&nbsp;Qiuyan Hu","doi":"10.1016/j.snb.2024.136904","DOIUrl":"10.1016/j.snb.2024.136904","url":null,"abstract":"<div><div>The electrochemical sensor shows great promise in the detection of trace Hg(Ⅱ), of which the performance could be significantly enhanced by the functionalized material with the merits of effective and ultrafast capture for Hg(Ⅱ). In this study, the novel metal coordination polymers (MCPs) was synthesized by the 2-thio-barbituric acid as the organic ligand to modify the electrochemical sensor to address this issue. The as-prepared material was composed of regular flakes with a polyhedral and well-defined crystal structure, the successful loading of the abundant sulfurous group (7.49 % of the total mass) endowed the Zn-TBA with the capability to efficiently uptake (97.4–99.9 %) Hg(Ⅱ) at 500 mg/L under a wider pHs as well as the high content of coexisting cations and anions, highlighted the excellent capacity, strong environmental adaptability, and selectivity. Notably, the superfast mass transfer was revealed in the adsorption kinetics test, in which the Hg(Ⅱ) content was effectively reduced from 50 mg/L to 14 μg/L within 20 s to meet the national emission standards. Based on this, Zn-TBA modified the carbon cloth (Zn-TBA/CC) and the glass carbon electrode (Zn-TBA/GCE) was prepared, thereinto, the current response of Zn-TBA/CC was approximately 60 times that of the pristine electrode. A strong positive correlation (R²=0.998) relationship could be attained across the content of 0.25–3 μM, achieving the detection limit to be 0.29 μM. The impressive performance was maintained in the complex matrices and regeneration test. In addition, Zn-TBA/CC exhibited better physical properties, charge transfer ability, and sensing properties than Zn-TBA/GCE, highlighting the advantages of CC as a flexible sensor substrate. Finally, the stable recovery (90–98.5 %) in the spiked actual surface water confirmed that the two established methods possessed a promising application potential in capturing and detecting the trace Hg(Ⅱ) with high accuracy and precision. This study extended the application of MCPs in the electrochemical sensor and provided an available analysis method to trap and determine the metal content.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136904"},"PeriodicalIF":8.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142588831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel NIR-activatable and photodegradable mitochondria-targeted nano-photosensitizer for superior photodynamic therapy","authors":"Chen Huang ,&nbsp;Xiaojie Ren ,&nbsp;Qingxin Chen ,&nbsp;Jie Zhang ,&nbsp;Shenglong Gan ,&nbsp;Yin Jiang ,&nbsp;Liu Yang ,&nbsp;Hongyan Sun","doi":"10.1016/j.snb.2024.136896","DOIUrl":"10.1016/j.snb.2024.136896","url":null,"abstract":"<div><div>Photodynamic therapy (PDT) has garnered significant interest as a promising approach for cancer treatment due to its effectiveness, versatility, and non-invasiveness. However, its clinical application is hindered by several factors, such as suboptimal efficacy, poor targeting capabilities, shallow penetration depth, and safety concerns. Herein, we introduce <strong>B-SQ</strong>, a novel nano-photosensitizer that addresses these challenges. <strong>B-SQ</strong> is a self-assembling, cancer mitochondria-targeting agent that is both activatable and photodegradable, designed by strategically modifying a near-infrared fluorescent squaraine dye. We extensively assessed the PDT potential of <strong>B-SQ</strong> in various cancer cell models and explored the molecular mechanisms of PDT-induced cell death. Importantly, <strong>B-SQ</strong> demonstrated high specificity for cancer cells, and its PDT efficiency was activatable, with near-infrared fluorescence to distinguish cancer cells from normal cells. This work provides the first evidence of <strong>B-SQ</strong> inducing the intrinsic mitochondrial apoptosis pathway and cell cycle arresting in cancer cells via PDT. Additionally, its photodegradable nature is likely to minimize side effects after treatment. This research introduces an innovative class of nano-photosensitizers that are activatable, targeting cancer mitochondria, and are photodegradable with near-infrared fluorescence. These qualities present new possibilities for safer and more effective clinical cancer treatments in the future.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136896"},"PeriodicalIF":8.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142579957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CoOOH nanoflake as supporter of CRISPR-Cas12a system for facilitated imaging of miRNA-21 in cells","authors":"Zhenhua Liu,&nbsp;Shushu Zhou,&nbsp;Dailiang Zhang,&nbsp;Minghui Yang","doi":"10.1016/j.snb.2024.136897","DOIUrl":"10.1016/j.snb.2024.136897","url":null,"abstract":"<div><div>The aberrant expression of miRNA-21 in cells is frequently associated with the occurrence and metastasis of tumors. The CRISPR-cas12a system has been developed for in vitro detection of miRNA due to its distinctive signal amplification characteristics. But its intricate composition and challenges in cellular delivery have limited its application in cell imaging. In this study, we designed an electrostatic adsorption-based probe by combining positively charged cobalt hydroxyl oxide (CoOOH) nanoflakes with negatively charged CRISPR-cas12a system which allows for detection and intracellular imaging of miRNA-21. This method involves using ascorbic acid to reduce CoOOH nanoflakes into Co²⁺, which subsequently releases the active form of CRISPR-Cas12 for detecting miRNA-21. The probe has a linear detection range for miRNA-21 from 0.5 pM-50 pM and 100 pM-20 nM, with a detection limit of 0.32 pM. It also shows good selectivity, accuracy, good reproducibility and biocompatibility for miRNA-21 analysis. In subsequent cell experiments, the probe exhibits low cytotoxicity and remarkable cell imaging capabilities. Therefore, this probe offers an innovative approach for miRNA-21 analysis.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136897"},"PeriodicalIF":8.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142580356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cavity-enhanced Faraday rotation spectroscopy for interference-free measurement of OH radical at 2.8 μm","authors":"Minh Nhut Ngo ,&nbsp;Tong Nguyen-Ba ,&nbsp;Nicolas Houzel ,&nbsp;Cécile Coeur ,&nbsp;Dorothée Dewaele ,&nbsp;Fabrice Cazier ,&nbsp;Weixiong Zhao ,&nbsp;Weidong Chen","doi":"10.1016/j.snb.2024.136901","DOIUrl":"10.1016/j.snb.2024.136901","url":null,"abstract":"<div><div>An instrument based on cavity-enhanced Faraday rotation spectroscopy (CE-FRS) operating at 2.8 μm has been developed for interference-free measurement of OH radicals in the laboratory. By off-axis coupling of a continuous-wave laser into a high finesse optical cavity, FRS signal is obtained from balanced detection of time-integrated light intensity leaking out of the cavity in the presence of magnetic field. Radio-frequency white noise (5–520 MHz) was injected into laser current which reduced intensity fluctuations in cavity transmission, thus improved the signal-to-noise ratio of the spectroscopic signal by a factor of 2. The setup provides a simple and robust spectroscopic instrument for in-situ and highly-selective detection of paramagnetic species. We demonstrated the instrument’s capabilities using OH radical with concentration in the range of 10¹² molecule.cm⁻³, generated by microwave discharge of water vapor at low pressure. The CE-FRS instrument exhibited a limit of detection of ∼ 10¹⁰ molecule.cm⁻³ in an integration time of 20 s, which is enhanced by a factor of 2.5 compared to cavity-enhanced wavelength modulation spectroscopy involving an off-axis integrated cavity output spectroscopy approach. A time-resolved FRS signal was recorded in a pulsed microwave discharge regime, giving a millisecond time resolution for the measurement of OH concentration profile. The developed instrument provides a potential analytical tool for the measurement of OH concentration for chemical kinetic study in reactor cells.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136901"},"PeriodicalIF":8.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142588829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An antibiofouling electrochemical sensor consisting of amphiphilic copolymer on CNT-COOH electrodes for point-of-care monitoring of propofol blood concentration in surgical patients","authors":"Shan Wang ,&nbsp;Chunguang Kuai ,&nbsp;Qianwen He ,&nbsp;Feng He ,&nbsp;Bingrui Xiong ,&nbsp;Li Li ,&nbsp;Xue Ke ,&nbsp;Yuzheng Guo ,&nbsp;Mian Peng","doi":"10.1016/j.snb.2024.136890","DOIUrl":"10.1016/j.snb.2024.136890","url":null,"abstract":"<div><div>The monitoring of propofol (PPF) blood concentration is vital for the safety of intravenous anesthesia. Electrochemical sensors represent a powerful tool for the detection of PPF. Our previous research has demonstrated that CNT-COOH electrodes exhibit advantages in resisting electrochemical fouling from PPF oxidation products. Nevertheless, biofouling remains a critical challenge hindering the clinical transformation of PPF electrochemical sensors. To address this issue, we employed in-situ electrodeposition to deposit zwitterionic sulfobetaine methacrylate (SBMA) and overoxidation polydopamine (OPDA) onto CNT-COOH screen-printed electrodes (SPEs) and proposed an innovative CNT-COOH/OPDA-SBMA (CNT-COOH/OPS) amphiphilic antifouling biosensor. This amphiphilic electrode, featuring both hydrophilic and hydrophobic properties, demonstrated excellent antifouling performance through electrochemical characterization in ex-vivo blood assays, coupled with commendable sensitivity, stability, and selectivity. CNT-COOH/OPS SPEs were also applied in point-of-care monitoring of the PPF blood concentrations in animals and clinical surgical patients and exhibited good accuracy and linearity (Ipa=0.0084c+0.4458, R²=0.995) with a limit of detection of 2.948 μM. Further, the electrodes demonstrated a high recovery and the blood concentrations determined by electrochemical detection had robust negative correlation with the depth of anesthesia. Therefore, CNT-COOH/OPS SPEs hold promise as a significant tool in point-of-care monitoring of PPF, offering patients safer and more personalized medical care.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136890"},"PeriodicalIF":8.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142580354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Dandelion” magnetic bead-driven CDs@CeO2 based-multifunctional nanoplatform for tri-modal ultrasensitive detection and efficient eradication of pathogenic bacteria","authors":"Ying Zhan ,&nbsp;Hui Li ,&nbsp;Yuwei Ren ,&nbsp;Yang Wang ,&nbsp;Rui Jiao ,&nbsp;Xiyan Zhang ,&nbsp;Xiaoyu Yu ,&nbsp;Xin Zhang ,&nbsp;Jianghua Cheng ,&nbsp;Yingwang Ye","doi":"10.1016/j.snb.2024.136903","DOIUrl":"10.1016/j.snb.2024.136903","url":null,"abstract":"<div><div>To address the challenge of foodborne pathogens, we have developed an ultra-sensitive tri-modal detection and effective pathogen eradication strategy utilizing a “dandelion” magnetic bead-driven cerium dioxide doped with carbon dots (CDs@CeO₂) multifunctional nanoplatform. Aptamer (Apt) modification CDs@CeO₂ specifically recognizes and binds to the outer membrane proteins of <em>Salmonella typhimurium</em> (<em>S. typhimurium</em>). Concurrently, mannose-functionalized dandelion-like magnetic beads selectively recognize adhesins of <em>S. typhimurium</em>, avoiding competition with Apt for binding sites and leveraging the strong bacterial capture capability for high-sensitivity detection. The prepared CDs@CeO₂ has been demonstrated to exhibit favourable fluorescence characteristics. In the presence of H₂O₂, CDs@CeO₂ is capable of oxidising colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxide, displaying exceptional photothermal properties. CDs@CeO₂ have the potential to generate •OH through a Fenton-like reaction, which could directly damage cells. Notably, coupling the photosensitizer Ce6 to CDs@CeO₂ and subsequent illumination generates ¹O₂, which synergistically eradicates bacteria with •OH. Detection limits of the fluorescence, colorimetry, and photothermal methods were 3.85 cfu/mL, 9.44 cfu/mL, and 5.00 cfu/mL, respectively. The platform successfully achieves precise detection of <em>S. typhimurium</em> in actual pre-prepared food samples through fluorescence, colorimetry, and photothermal tri-modal signals, and effectively kills bacteria, achieving trace detection and source control.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136903"},"PeriodicalIF":8.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142580377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co ions doping enhances n-butanol sensing performance of In2O3 nanospheres","authors":"Zhiguo Yang ,&nbsp;Xingtai Chen ,&nbsp;Qiuying Chen ,&nbsp;Jiayi Qu ,&nbsp;Yujun Guo ,&nbsp;Kaiwen Zhou ,&nbsp;Tianren Wang ,&nbsp;Davoud Dastan ,&nbsp;Xiaoning Wang ,&nbsp;Feifei Wang ,&nbsp;Xiaoming Tan ,&nbsp;Xi-Tao Yin ,&nbsp;Xiaoguang Ma","doi":"10.1016/j.snb.2024.136898","DOIUrl":"10.1016/j.snb.2024.136898","url":null,"abstract":"<div><div>In this work, In₂O₃ nanospheres were synthesized using urea as a carbon template and modified with Co ions. The synthesized materials were characterized using various instruments to obtain information about the elemental composition and morphology of the materials. The results showed that Co ions replaced the lattice position of In ions and synthesized nanospheres aggregated from 20 nm particles. The results of gas sensitivity tests on all the samples showed that the response to 50 ppm n-butanol at 250 °C reached 540 when Co was doped up to 5 at%. In comparison, the sensor showed very low response to benzene gas with good selectivity. The high response of the sensor to n-butanol is due to the increase in vacancy oxygen content and redox reactions due to the multivalent nature of Co ions. The article concludes with a detailed analysis of the sensing mechanism.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136898"},"PeriodicalIF":8.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142580254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-enzymatic (AChE/ChOx) colorimetric detection of organophosphorus pesticides based on controllable nanoparticles supported by FeCoCu flower-like structure with peroxidase-like activity","authors":"Ziyu Yan ,&nbsp;Jiali Zhong ,&nbsp;Ziwen Liao ,&nbsp;Peng Xu ,&nbsp;Ping Qiu","doi":"10.1016/j.snb.2024.136878","DOIUrl":"10.1016/j.snb.2024.136878","url":null,"abstract":"<div><div>Organophosphorus pesticides (OPs) are widely used but easily persist in the environment, which can impact ecological and biological health. In this paper, the nanoparticles supported by FeCoCu flower-like structure (FeCoCu-FN) was successfully synthesized when the molar ratio of Fe:Co:Cu is 0.6:0.4:0.6, which exhibits strong peroxidase-like activity at high <em>V</em>_<em>m</em> (266.7×10⁻⁸ M/s). Combined with 3,3′,5,5′-tetramethylbenzidine (TMB), two types of colorimetric sensors, acetylcholinesterase (AChE) method and choline oxidase (ChOx) method, were firstly proposed in the same reaction system for detection of OPs based on FeCoCu-FN. The comparisons of both the two assays were conducted in details, such as cost, sensitivity, speed and stability. Moreover, the inhibition effects between parathion methyl (PM) and the two enzymes (AChE and ChOx) were predicted and contrasted using the molecular docking method. It can be found that the free binding energy of AChE with PM is smaller than that of ChOx with PM, which indicates that PM is more easily bound to AChE. On the other hand, the inhibition constant of AChE with PM is smaller than that of ChOx with PM, which illustrates the stronger inhibition effect of PM on AChE. The AChE single-enzyme method has higher sensitivity (LOD=0.011 ng/mL), which was consistent with the result of molecular docking. Two analysis methods were used to detect PM in real samples, and the detection results were validated using <em>t</em>-test and <em>F</em>-test. The results showed that there was no significant difference in accuracy and mean between the two methods, and both methods were reliable.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136878"},"PeriodicalIF":8.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142588864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}