{"title":"Electrochemical aptasensor for thrombin based on polyfunctionalized dendritic nanodevice as natural enzyme-free labeling element","authors":"Anabel Villalonga , Mihai-Cristian Fera , Alicia Piedras , Alfredo Sánchez , Diana Vilela , Irene Ojeda , Yaiza Belacortu , Paloma Martínez-Ruíz , Reynaldo Villalonga","doi":"10.1016/j.snb.2025.137977","DOIUrl":"10.1016/j.snb.2025.137977","url":null,"abstract":"<div><div>A novel dendritic hybrid nanomaterial with biorecognition and catalytic properties was synthesized by covalent attachment of specific aptamers on the surface of poly(amidoamine) G-4.5 dendrimers and further <em>in situ</em> growth of Pt nanoparticles encapsulated into the polymeric cavities. This nanodevice was employed as natural enzyme-free labeling element to design an amperometric aptasensor with sandwich-type architecture for the specific detection of thrombin, by using screen-printed carbon electrodes functionalized with Au nanoparticles and capture aptamers as transduction interface. The aptasensor was employed to detect the cardiovascular biomarker in the broad range from 1.0 pM to 1.5 nM, with detection limit of 200 fM. This electroanalytical device also showed high specificity, reproducibility and stability, retaining 92 % of its initial biosensing capacity after 50 days of storage at 4ºC. The aptasensor was successfully employed to quantify thrombin in spiked human serum samples.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 137977"},"PeriodicalIF":8.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144066555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jingran Sun , Kaiyue Hu , Yufang Hu , Jiayun Chen , Anqi Chen , Luhong Wen , Zhihe Qing
{"title":"Non-competitive inhibition of Ag(I) catalysis by coenzyme A: A novel strategy for detecting histone acetyltransferase activity in gastric cancer circulating tumor cells","authors":"Jingran Sun , Kaiyue Hu , Yufang Hu , Jiayun Chen , Anqi Chen , Luhong Wen , Zhihe Qing","doi":"10.1016/j.snb.2025.137959","DOIUrl":"10.1016/j.snb.2025.137959","url":null,"abstract":"<div><div>This study introduces a novel method for detecting histone acetyltransferase (HAT) activity in gastric cancer circulating tumor cells (CTCs) by leveraging coenzyme A (CoA)-mediated non-competitive inhibition of silver ion (Ag(I)) catalysis. The interactions between CoA, Ag(I), and the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) were thoroughly examined, leading to the development of a highly sensitive and specific assay for HAT detection. Notably, this method utilizes Ag(I) to catalyze the oxidation of TMB without the need for H₂O₂, simplifying the reaction system. Our results show that CoA effectively inhibits the catalytic activity of Ag(I) in a non-competitive fashion, offering a robust means to quantify HAT activity in CTCs. By utilizing UV-Vis spectroscopy and a smartphone-based RGB platform, this method exhibited remarkable selectivity and sensitivity, with a detection limit of 0.033 ng/mL (S/N = 3). Comprehensive analysis across various cell types, including normal cells, gastric cancer cells, and simulative CTCs, revealed that HAT activity was highest in CTCs, underscoring their critical role to some extent in tumor metastasis. Furthermore, stimulation with epidermal growth factor (EGF) and tumor necrosis factor-α (TNF-α) demonstrated distinct HAT activity patterns in different cells, highlighting their differential responses to growth and inflammatory signals. This method can also be adapted to other biosensing platforms by altering metal ions and inhibitory components, showcasing its versatility and potential for real-world applications in cancer diagnostics and metastasis monitoring.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 137959"},"PeriodicalIF":8.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xin Miao, Yilin Jiang, Wenjing Liu, Chunxiao Wu, Feng Li , Ming Zhang
{"title":"An electrostatic potential modulation strategy for fluorescent probe coupled with deep learning enabling ultrafast and visual detection of meat freshness","authors":"Xin Miao, Yilin Jiang, Wenjing Liu, Chunxiao Wu, Feng Li , Ming Zhang","doi":"10.1016/j.snb.2025.137974","DOIUrl":"10.1016/j.snb.2025.137974","url":null,"abstract":"<div><div>Biogenic amines (BAs), as the metabolic byproducts of proteolysis, are essential indicators in assessing the freshness of meat. Excessive BAs intake can lead to a range of diseases, highlighting the importance of detection BAs in a rapid, on-site, accurate way. To this aim, a strategy of modulating sensing performance of the fluorescence probes is put forward and a portable detection platform is built up by a smartphone as well as a deep convolutional neural network (DCNN). Here, three fluorescence probes applying diphenylamine (DPA), thiodiphenylamine (PTZ), and carbazole (Cz) as the fluorophore cores is synthesized, which feature a specific recognition site of “N-H” and stepwise increased electrostatic potential (ESP). It is found that, <strong>CF</strong><sub><strong>3</strong></sub><strong>Cz</strong>, with the highest ESP, enables superior detection performance, including a low detection limit (11 ppb), rapid response (<5 s) and noticeable bathochromic shifts (90 nm). Further, a portable smartphone-based fluorescence platform allows for the visual recognition and on-site quantitative detection of BAs. By combining with DCNN, this platform is able to predict meat freshness with an accuracy of 99.7 %. This work not only provides a design methodology by precisely modulating ESP, but also presents an intelligent detection platform for nondestructive assessment of BAs and meat freshness.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 137974"},"PeriodicalIF":8.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lichao Liu , Teng Fei , Hongran Zhao , Pengjia Qi , Tong Zhang
{"title":"High-performance flexible gas sensor for ultrasensitive ammonia detection via straightforward all-in-one synthesis technology","authors":"Lichao Liu , Teng Fei , Hongran Zhao , Pengjia Qi , Tong Zhang","doi":"10.1016/j.snb.2025.137958","DOIUrl":"10.1016/j.snb.2025.137958","url":null,"abstract":"<div><div>In this work, we employed straightforward all-in-one synthesis technology to fabricate room temperature flexible ammonia sensors that integrate sensitive materials and flexible substrates. The sensitive sensor material was composed of a nontoxic and environmentally friendly cellulose-based material and a biomass acid, which was selected to ensure environmental safety during exhaled gas detection. The optimized sensor displayed excellent selectivity for NH<sub>3</sub> over a range of interfering gas species, achieving a significant NH<sub>3</sub> response (Za/Zg = 5.30) for 20 ppm NH<sub>3</sub>, along with a low actual detection limit of 300 ppb at room temperature under high humidity conditions (80 % RH). As flexible devices, these sensors were capable of working under deformation conditions and have non-degenerated sensing performance. The humidity-activated NH<sub>3</sub> sensing mechanism was extensively examined using complex impedance spectroscopy (CIS) and quartz crystal microbalance (QCM) techniques. Simultaneously, experiments involving the detection of simulated exhaled gases were conducted to investigate the potential of the sensor in this field.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 137958"},"PeriodicalIF":8.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143979968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jodinio Lemena , Richard A. Harris, Hendrik C. Swart, Jacob J. Terblans, David E. Motaung
{"title":"Advances in the engineering of MXenes-based sensors: A transition towards advanced sensing technologies","authors":"Jodinio Lemena , Richard A. Harris, Hendrik C. Swart, Jacob J. Terblans, David E. Motaung","doi":"10.1016/j.snb.2025.137939","DOIUrl":"10.1016/j.snb.2025.137939","url":null,"abstract":"<div><div>MXenes are a two-dimensional group that have opened a new frontier in science, as their real-world applications are far-reaching, including batteries, supercapacitors, catalysts, electronics, and optics. MXenes possess adjustable electrical properties, high conductivity, and plenty of surface functional groups, compelling them to be extremely attractive for gas sensing applications. This review provides a comprehensive analysis of recent advancements in MXene-based gas sensors, emphasizing environmentally friendly synthesis methods that are replacing traditional HF etching. The properties of MXenes, encompassing their optical, thermal, mechanical, and gas-sensing characteristics, were discussed. Furthermore, the review examines modifications aimed at enhancing sensing capabilities, including functional group control, defect engineering, heterostructure formation, light assistance, and noble metal doping. Each enhancement method is systematically compared in terms of advantages and limitations. The advantages and limitations of each gas-sensing enhancement method were conveniently tabulated for ease of comparison. A computational analysis of the conductivity of an MXene based on the first principle DFT analysis on Ti<sub>3</sub>C<sub>2</sub>Tx is also discussed. A principle component analysis, which is used in machine learning and data analysis to identify patterns in complex datasets and identify which variables are most important, was discussed. The encore of this review discusses self-powered gas sensors, their integration into the Internet of Things (IoT), and the commercial viability and scalability of gas sensors, with a detailed analysis of their application in the fish market. The review concludes by addressing existing challenges and future directions in MXene-based gas sensors, underscoring the need for improved synthesis techniques, a better understanding of sensing mechanisms, and strategies for long-term material stability.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 137939"},"PeriodicalIF":8.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
María González-Gómez , Elizabeth Martínez Medina , Janez Kovač , Olga Casals , Mònica Martínez López , Elena Xuriguera-Martín , Cristian Fàbrega
{"title":"A low-cost colorimetric HKUST-1 sensor for ppm-level humidity detection","authors":"María González-Gómez , Elizabeth Martínez Medina , Janez Kovač , Olga Casals , Mònica Martínez López , Elena Xuriguera-Martín , Cristian Fàbrega","doi":"10.1016/j.snb.2025.137973","DOIUrl":"10.1016/j.snb.2025.137973","url":null,"abstract":"<div><div>Accurate detection of trace moisture is critical in various industries, from microelectronics to petrochemicals. While electrical humidity sensors are prevalent, optical methods offer superior sensitivity for low relative humidity (RH) detection, particularly in demanding applications requiring ppb sensitivity in harsh environments. This work explores the potential of HKUST-1, a metal-organic framework (MOF), as a colorimetric humidity sensor for ppm level detection across a broad range (0–80 % RH). By spraying HKUST-1 onto polyester substrates and coupling it with a low-cost, commercially available LED-photodiode module, we developed a simple, portable sensing platform. The observed color transitions, from dark blue to turquoise, correlate with the MOF's moisture adsorption mechanism, achieving a detection limit of 18 ppm H<sub>2</sub>O (< 0.05 % RH). Comprehensive characterization, including interferent gas studies, revealed significant color changes upon exposure to NH<sub>3</sub>, CO, and ethanol, while CO<sub>2</sub>, CH<sub>4</sub>, and N<sub>2</sub>O showed minimal interference. One-week stability tests demonstrated consistent sensor performance and full reversibility upon exposure to dry air at room temperature. This low-cost, sensitive, and easily fabricated HKUST-1-based sensor offers a promising alternative for humidity monitoring in diverse industrial applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 137973"},"PeriodicalIF":8.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chang Xue , Jinmei Fu , Tingting Tao , Shouhu Zhu , Bingsong Han , Yulong Wang , Yuhui Ye , Ruifeng Luo , Qin Ouyang , Bruce D. Hammock , Cunzheng Zhang
{"title":"Using designed polypeptides to develop novel phage-mediated triple antibody sandwich immunoassay for the specific detection of Escherichia coli O157:H7 in milk","authors":"Chang Xue , Jinmei Fu , Tingting Tao , Shouhu Zhu , Bingsong Han , Yulong Wang , Yuhui Ye , Ruifeng Luo , Qin Ouyang , Bruce D. Hammock , Cunzheng Zhang","doi":"10.1016/j.snb.2025.137964","DOIUrl":"10.1016/j.snb.2025.137964","url":null,"abstract":"<div><div>In this study, a phage-mediated triple antibody sandwich immunoassay (TAS-ELISA) was developed and exploited for the sensitive detection of <em>Escherichia coli</em> O157:H7 (<em>E. coli</em> O157:H7), which could be potentially tailored to individual serotypes testing. Bioinformatics analysis was conducted to elucidate the potential epitopes of <em>E. coli</em> secreted protein A (EspA) and Outer membrane protein A (OmpA). Peptide fragments were selected and designed to serve as antigens in the screening of phage-displayed antibody library, two nanobodies (Nbs) that exhibit specific capabilities for distinct epitopes recognition were obtained. Using monoclonal antibody as capture and two Nbs as tracer, a phage-mediated triple antibody sandwich immunoassay was developed. The results revealed that the limit of detection (LOD) for <em>E. coli</em> O157:H7 was 1.89 × 10<sup>3</sup> CFU/mL with no significant cross-reactivity to other bacterial, which was 41.8-fold higher than that of the sandwich immunoassay based on mAb. Furthermore, molecular docking provides insight into the specific binding interactions between these two distinct epitopes and nanobodies. Method validation revealed that the developed TAS-ELISA has great potential for the specific determination of <em>E. coli</em> O157:H7 in food and could be further refined for precise serotype discrimination by identifying multiple unique epitopes. This study provides an alternative strategy to precisely detect <em>E. coli</em> O157:H7 in food.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 137964"},"PeriodicalIF":8.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lixian Huang , Jinzhe Liang , Jiaqi Lu , Min He , Yidong Bin , Yanni Luo , Shulin Zhao , Shengqiang Hu , Liangliang Zhang
{"title":"A smart nanoprobe with simultaneously triggering reactive oxygen species and NIR-II fluorescence for enhanced chemodynamic/photodynamic therapy and ratiometric imaging- guided tumor resection","authors":"Lixian Huang , Jinzhe Liang , Jiaqi Lu , Min He , Yidong Bin , Yanni Luo , Shulin Zhao , Shengqiang Hu , Liangliang Zhang","doi":"10.1016/j.snb.2025.137971","DOIUrl":"10.1016/j.snb.2025.137971","url":null,"abstract":"<div><div>The integration of ratiometric fluorescence imaging in the second near-infrared (NIR-II) window with chemodynamic therapy (CDT) and photodynamic therapy (PDT) represents a promising strategy for the accurate diagnosis and efficient treatment of deep tumors. In this study, we developed a smart nanoprobe, D/UCNP-MB@SiO<sub>2</sub>-<sub>t</sub>AuCuNCs (D/UCMSA), with simultaneously triggering reactive oxygen species (ROS) and NIR-II fluorescence for enhanced chemodynamic/ photodynamic therapy and ratiometric imaging-guided tumor resection. Upon 980 nm laser irradiation, D/UCMSA emit up-converted light at 660 nm, which activates the loaded methylene blue (MB) to produce singlet oxygen (<sup>1</sup>O<sub>2</sub>) for PDT. Simultaneously, the down-converted emission at 1525 nm serves as a reference signal for ratiometric imaging. The D/UCMSA surface is decorated with copper-doped gold nanoclusters (<sub>t</sub>AuCuNCs) stabilized by triphenylphosphine-3,3′,3′′-trisulfonic acid, which undergo ligand exchange with overexpressed glutathione in the tumor microenvironment. Upon 808 nm laser excitation, this ligand exchange process activates fluorescence emission at 1085 nm. The incorporation of copper endows <sub>t</sub>AuCuNCs with peroxidase- and catalase-like activities, enabling the catalytic conversion of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) into hydroxyl radicals (·OH) and oxygen (O<sub>2</sub>) within the tumor microenvironment. This dual enzymatic activity amplifies the CDT efficacy in tumor treatment and reshapes the tumor microenvironment, providing sufficient O<sub>2</sub> for <sup>1</sup>O<sub>2</sub> generation during PDT and enhancing the efficiency of combined CDT/PDT. Following with orthotopic injection of the D/UCMSA, irradiation with 808 nm and 980 nm lasers successfully enabled precise tumor resection guided via ratiometric NIR-II fluorescence imaging, combined with effective CDT/PDT. This study demonstrates a novel approach to developing integrated nanoplatforms for accurate tumor diagnosis and efficient treatment.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 137971"},"PeriodicalIF":8.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jie Luo , Bin Yang , Chengchun Zi , Zhen Ren , Wei Huang , Shijia Ding , Farhan Afzal , Yongjie Xu , Yan Zha
{"title":"Highly atom-economic strip for respiratory syncytial virus detection using an entropy-driven circuit-based one-stone-two-birds strategy","authors":"Jie Luo , Bin Yang , Chengchun Zi , Zhen Ren , Wei Huang , Shijia Ding , Farhan Afzal , Yongjie Xu , Yan Zha","doi":"10.1016/j.snb.2025.137963","DOIUrl":"10.1016/j.snb.2025.137963","url":null,"abstract":"<div><div>Lateral flow assays (LFAs) are rapid, inexpensive, and widely used for point-of-care testing (POCT) of viral RNA. However, traditional capture probes, which bind only one type of reporter tag in a single sensing zone, limit amplification efficiency and compatibility with diverse amplification methods. We developed a multi-signal, reverse-transcription-free lateral flow assay for detecting respiratory syncytial virus (RSV) RNA fragments. This assay leverages a chimeric tetrahedral probe combined with an entropy-driven, 'one-stone-two-birds' strategy for enhanced signal amplification. The tetrahedral chimera carries two sorts of capture probes that recognize and capture different signal strand inputs. Entropy-driven circuits (EDC) generate abundant waste chains via a self-feedback cascade reaction, including two biotin-labeled single strands and one duplex. As reporter tags, the two biotinylated single-stranded chains can be simultaneously matched and captured by tetrahedral chimera during one time of sample flow, bridging horseradish peroxidase-coupled streptavidin-modified gold nanoparticles onto the strip to induce coloration. In this process, every EDC cycle can achieve dual signal outputs, akin to \"killing two birds with one stone\" in the atom-economic signal amplification. Furthermore, the gold nanoparticles carry amounts of horseradish peroxidase, serving as catalytic amplified labels to enhance the colorimetric signal. The strip achieves a broad linear dynamic range and a detection limit of 0.1fM with high specificity for RSV detection. Thus, this lateral flow assay realizes a high atom economy in amplification reaction and offers a rapid, reliable, sensitive, and widely accessible tool for on-site visual detection of viral nucleic acids.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 137963"},"PeriodicalIF":8.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143940529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dongwoo Seo , Gyubin Park , Jaehyun Kim , Taesung Kim , Jungyul Park
{"title":"Enhancement of nanofluidic ionic current via plasmon-mediated effect","authors":"Dongwoo Seo , Gyubin Park , Jaehyun Kim , Taesung Kim , Jungyul Park","doi":"10.1016/j.snb.2025.137957","DOIUrl":"10.1016/j.snb.2025.137957","url":null,"abstract":"<div><div>Localized surface plasmon resonance (LSPR) has been extensively used in various applications, but the comprehension of its role in plasmon-mediated photochemical and photothermal effects on ionic currents is still in its infancy. To improve the efficiency of optofluidic systems, it is imperative to investigate the ion transport behavior influenced by LSPR within electrolytes. In this study, we employ a 3D nanochannel network membrane (NCNM) integrated micro/nanofluidic device to elucidate the mechanisms underlying plasmon-mediated ionic current enhancement. By analyzing ionic currents generated by laser irradiation versus thermal heating, our results indicate that the photoelectrochemical effect, which increases the space charge density, plays a more significant role in ionic current enhancement than pure thermal effects. Furthermore, our investigation of various structural parameters shows that optimizing laser power absorption through the 3D NCNM is crucial for effective ionic current generation. This research represents a fundamental step in the understanding of plasmon-mediated ionic transport mechanisms and provides insights into efficient light-to-ionic transport conversion. These findings have potential for a wide range of applications, including photoinduced energy harvesting devices, ionic pumps and wavelength-selective nanochannels, such as fluidic rhodopsin systems.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 137957"},"PeriodicalIF":8.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143940530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}