Sensors and Actuators B: Chemical最新文献

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Dual-signal immunosensor based on aggregation-induced electrochemiluminescence resonance energy transfer of La-MOF and low cathodic excitation potential of g-C3N4@AuNPs for the quantitation of human serum amyloid A 基于 La-MOF 的聚集诱导电化学发光共振能量转移和 g-C3N4@AuNPs 的低阴极激发电位的双信号免疫传感器用于定量检测人血清淀粉样蛋白 A
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2024-11-12 DOI: 10.1016/j.snb.2024.136925
Shenglan Hu, Lixin Xu, Minlan Huang, Yusheng Wu, Xuemin Qin, Biyang Deng
{"title":"Dual-signal immunosensor based on aggregation-induced electrochemiluminescence resonance energy transfer of La-MOF and low cathodic excitation potential of g-C3N4@AuNPs for the quantitation of human serum amyloid A","authors":"Shenglan Hu,&nbsp;Lixin Xu,&nbsp;Minlan Huang,&nbsp;Yusheng Wu,&nbsp;Xuemin Qin,&nbsp;Biyang Deng","doi":"10.1016/j.snb.2024.136925","DOIUrl":"10.1016/j.snb.2024.136925","url":null,"abstract":"<div><div>We report on the development of a dual-signal electrochemiluminescence (ECL) immunosensor for the quantitation of human serum amyloid A (SAA). The immunosensor comprises g-C<sub>3</sub>N<sub>4</sub>@AuNPs as the cathode and a lanthanum-based metal organic framework (La-MOF) as the anode, the latter of which utilizes aggregation-induced electrochemiluminescence (AIECL) to overcome the otherwise poor ECL intensity of H<sub>4</sub>TBAPy ligands at the electrode surface. Furthermore, the rigid MOF structure significantly improved the fluorescence quantum yield, as well as the fluorescence and ECL emission, of the La-MOF compared to H<sub>4</sub>TBAPy. The g-C<sub>3</sub>N<sub>4</sub>@AuNPs exhibits an ultrathin nanosheet structure with a low cathodic excitation potential of −0.72 V. When the dual-signal ECL immunosensor was employed to detect SAA, the difference between the anodic and cathodic ECL signals exhibited a strong linear relationship with the logarithm of the SAA concentration in the range of 100 fg/mL–200 ng/mL, with a detection limit of 24.5 fg/mL.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136925"},"PeriodicalIF":8.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorescent probe for tumor imaging and prognostic assessment via multi-response to biothiols, viscosity, and pH values 通过对生物硫醇、粘度和 pH 值的多重响应进行肿瘤成像和预后评估的荧光探针
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2024-11-12 DOI: 10.1016/j.snb.2024.136926
Hailong Ma , Zhiqiang Luo , Jiale Ding , Dan Zhang , Feiyi Wang , Huili Yu , Wei Chen , Jun Ren , Erfei Wang
{"title":"Fluorescent probe for tumor imaging and prognostic assessment via multi-response to biothiols, viscosity, and pH values","authors":"Hailong Ma ,&nbsp;Zhiqiang Luo ,&nbsp;Jiale Ding ,&nbsp;Dan Zhang ,&nbsp;Feiyi Wang ,&nbsp;Huili Yu ,&nbsp;Wei Chen ,&nbsp;Jun Ren ,&nbsp;Erfei Wang","doi":"10.1016/j.snb.2024.136926","DOIUrl":"10.1016/j.snb.2024.136926","url":null,"abstract":"<div><div>Accurate tumor diagnosis and drug efficacy assessment are crucial aspects of cancer treatment. Tumor cells typically display aberrant levels of biothiols, higher viscosity, and lower acidity compared to normal cells. Thus, precise monitoring of biothiol levels in the intricate cellular environment is essential for early tumor detection. We have developed a novel near-infrared (NIR) fluorescence probe <strong>AX-DNBS</strong> for the simultaneous detection of biothiols, viscosity, and pH through multi-channel imaging. With the treatment of biothiol, <strong>AX-DNBS</strong> triggers the release of AX-OH, resulting in enhanced fluorescence emission at 710 nm. Both <strong>AX-DNBS</strong> and AX-OH exhibit sensitive responses to changes in viscosity, with fluorescence emissions at 475 nm and 640 nm, respectively. In the pH range of 3–10, an increase in alkalinity leads to a ratiometric fluorescence signal of AX-OH with a significant emission red-shift from 640 nm to 710 nm. Significantly, in subsequent biological trials, <strong>AX-DNBS</strong> proved effective in monitoring alterations in biothiol levels, viscosity, and pH within live cells, facilitating the diagnosis of tumors and the evaluation of the therapeutic effects of paclitaxel in tumor mice models.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136926"},"PeriodicalIF":8.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An ultrasensitive fluorescent strategy for 17β-estradiol based on aptamer and isothermal exponential amplification reaction 基于适配体和等温指数扩增反应的 17β-estradiol 超灵敏荧光策略
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2024-11-12 DOI: 10.1016/j.snb.2024.136929
Di Liu , Xuelian Chen , Yaping Li , Guiping Sun , Jingwen Gao , Hailiang Nie , Cuiping Li
{"title":"An ultrasensitive fluorescent strategy for 17β-estradiol based on aptamer and isothermal exponential amplification reaction","authors":"Di Liu ,&nbsp;Xuelian Chen ,&nbsp;Yaping Li ,&nbsp;Guiping Sun ,&nbsp;Jingwen Gao ,&nbsp;Hailiang Nie ,&nbsp;Cuiping Li","doi":"10.1016/j.snb.2024.136929","DOIUrl":"10.1016/j.snb.2024.136929","url":null,"abstract":"<div><div>The abuse of 17β-estradiol (E2) in breeding and the excretion into the environment by human beings result in exposure of E2 to humans. Excessive levels of E2 can cause an adverse effect on the human endocrine systems. Therefore, an efficient and sensitive method for the analysis of E2 is required. Herein, based on the specific capture of aptamer, the digestion of Exonuclease III, and efficient amplification of isothermal exponential amplification reaction (EXPAR), a highly sensitive and selective strategy is established for E2 analysis. E2 binds to the aptamer due to their high affinity, preventing the complementary DNA (cDNA) of the aptamer from hybridizing with it. Thus, the cDNA remains unbound and freely available in the solution. Then, Exonuclease III digests the cDNA-aptamer complex, leaving the free cDNA intact. cDNA serves as primers to trigger EXPAR with the help of Bst 2.0 WarmStart DNA polymerase and Nt.BstNBI nicking enzyme, achieving sensitive detection of E2. Without E2, cDNA hybridizes with the aptamer to form double-stranded DNA, which is degraded by Exonuclease III. As a result, the amplification reaction fails to initiate. The approach shows a linear range of 100 aM-10 pM, spanning 5 orders of magnitude, with the limit of detection as low as 36 aM. In addition, the selectivity and reproducibility are great, and the recoveries in tap water and milk ranges from 99.4 % to 117.3 %, suggesting good practicability. This method may also provide a new possibility for sensitive detection of other small-molecule targets via using the corresponding aptamers.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136929"},"PeriodicalIF":8.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Functionalized DNA mimic of GFP based on the molecule-activated chromophore derivative for hydrogen peroxide detection 基于分子激活发色团衍生物的过氧化氢检测 GFP 功能化 DNA 模拟物
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2024-11-12 DOI: 10.1016/j.snb.2024.136927
Meiyun Chen , Jinrong Li , Ziqing Qiu , Yanfei Zhang , Chunfen Zhang , Peng Yin , Xingyu Luo
{"title":"Functionalized DNA mimic of GFP based on the molecule-activated chromophore derivative for hydrogen peroxide detection","authors":"Meiyun Chen ,&nbsp;Jinrong Li ,&nbsp;Ziqing Qiu ,&nbsp;Yanfei Zhang ,&nbsp;Chunfen Zhang ,&nbsp;Peng Yin ,&nbsp;Xingyu Luo","doi":"10.1016/j.snb.2024.136927","DOIUrl":"10.1016/j.snb.2024.136927","url":null,"abstract":"<div><div>Hydrogen peroxide (H₂O₂) plays a pivotal role in both physiological and pathological processes, which attracted considerable attention to its content analysis. Detection of certain small molecules based on DNA/RNA mimics of green fluorescent protein (GFP mimics) has been preferentially constructed due to its high specificity, excellent optical properties, and high stability. However, there is a dearth of functional GFP mimics that can be directly activated by these targets. Herein, an H<sub>2</sub>O<sub>2</sub>-activated DNA mimic of GFP (HDG) has been proposed through primarily remolding the GFP chromophore derivative named 3,5-difluoro-4-hydroxybenzylidene imidazolidinone (DFHBI) with phenyl boronate group (PB-DFHBI) and then forming the HDG by adding the truncated Lettuce DNA aptamer (tLet-10) to reveal the specifically fluorescent response toward H₂O₂. In this sensing method, the fluorescence of PB-DFHBI is markedly declined with PB group lock by restricting its intramolecular charge transfer (ICT) process, while the presence of H<sub>2</sub>O<sub>2</sub> will activate a cascade reaction to remove the PB group in HDG, resulting in a high-contrast and turn-on fluorescence. The unique emitting mechanism triggered by H₂O₂ endows HDG with stable, rapid response, high sensitivity, and specific detection performances, exhibiting a low detection limit of 0.59 μM and a comprehensive linear range of 1–1000 μM. Moreover, HDG was allowed to realize the reliable and accurate analysis of spiked H<sub>2</sub>O<sub>2</sub> in beverage and water samples. It is believed that the well-designed HDG will provide a promising way to establish new sensing strategies for certain target analyses and expand their applications of GFP mimics-based sensing platforms.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136927"},"PeriodicalIF":8.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
UV light-activated gas mixture sensing by ink-printed WS2 layer 通过墨水印刷 WS2 层实现紫外光激活的混合气体传感
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2024-11-12 DOI: 10.1016/j.snb.2024.136923
Katarzyna Drozdowska , Janusz Smulko , Artur Zieliński , Andrzej Kwiatkowski
{"title":"UV light-activated gas mixture sensing by ink-printed WS2 layer","authors":"Katarzyna Drozdowska ,&nbsp;Janusz Smulko ,&nbsp;Artur Zieliński ,&nbsp;Andrzej Kwiatkowski","doi":"10.1016/j.snb.2024.136923","DOIUrl":"10.1016/j.snb.2024.136923","url":null,"abstract":"<div><div>We fabricated a sensing layer from ink-printed WS<sub>2</sub> flakes and utilized it for UV-activated gas sensing. The optical imaging of the structure made by repeated printing revealed the continuous layer comprising sub-µm flakes, confirmed independently by small-area AFM images (1×1 µm<sup>2</sup>). The activity of the sensing surface was investigated locally <em>via</em> AFM scanning of the surface with a polarized probing tip. The results indicated that the applied UV light amplifies the existing conducting paths in the dark. These hot spots are associated with the sensing activity of the WS<sub>2</sub> surface (local adsorption-desorption centers). Gas sensing experiments revealed that the DC resistance of the WS<sub>2</sub> sensor changes in the opposite direction for increasing concentrations of NO<sub>2</sub> and NH<sub>3,</sub> which correlates with the electron-accepting and electron-donating properties of these species. On the contrary, low-frequency noise intensifies gradually in both gases, and relative changes in noise responses are higher than DC resistance responses for all investigated concentrations. The lowest detection limit obtained was 103 ppb from DC responses for NO<sub>2</sub> and 168 ppb from noise responses for NH<sub>3</sub>. The studies of sensing responses for mixtures of the mentioned target gases revealed that the amplitude of resistance fluctuations is not a direct summation of spectra obtained for pure compounds. Such an effect observed for mixed gases indicates that the intermittent reactions between both species before adsorbing at the sensing surface or in the adsorption centers impact their detection.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136923"},"PeriodicalIF":8.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In-situ electrochemical synthesis of Ni/Ni(OH)2/molecularly imprinted polymer nanocomposite for high-performance glucose detection 原位电化学合成用于高性能葡萄糖检测的 Ni/Ni(OH)2/分子印迹聚合物纳米复合材料
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2024-11-10 DOI: 10.1016/j.snb.2024.136921
Rourke Sylvain , Grace Dykstra , Asky Fungura , Smitha Rao , Yixin Liu
{"title":"In-situ electrochemical synthesis of Ni/Ni(OH)2/molecularly imprinted polymer nanocomposite for high-performance glucose detection","authors":"Rourke Sylvain ,&nbsp;Grace Dykstra ,&nbsp;Asky Fungura ,&nbsp;Smitha Rao ,&nbsp;Yixin Liu","doi":"10.1016/j.snb.2024.136921","DOIUrl":"10.1016/j.snb.2024.136921","url":null,"abstract":"<div><div>This study presents a novel non-enzymatic glucose sensor that synergistically combines the high catalytic activity of nickel hydroxide (Ni(OH)<sub>2</sub>) with the selective recognition of molecularly imprinted polymers (MIPs). The sensors were fabricated through an entirely in-situ synthesis directly on the electrode, comprising electrodeposition and oxidation of Ni/Ni(OH)<sub>2</sub> nanoparticles and electropolymerization of a glucose-imprinted MIP layer using pyrrole and 3-aminophenyl boronic acid. The integrated MIP layer significantly enhanced selectivity against common interferents while amplifying glucose sensitivity. The resulting sensor demonstrated a high sensitivity of 1802 μA mM<sup>−1</sup> cm<sup>−2</sup> with a linear range from 0.04 to 2.6 mM. Notably, the sensor exhibited remarkable stability, retaining 97.2 % of its original sensitivity after 6 months of room-temperature storage. To extend the linear range, Nafion coatings at two concentrations were applied, achieving ranges up to 0.04–11.6 mM with adjusted sensitivities. This innovative approach, leveraging MIPs to provide selectivity to electrocatalytic nanomaterials, offers a promising strategy for developing high-performance non-enzymatic sensors for glucose and other biomolecules in diabetes monitoring and beyond.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136921"},"PeriodicalIF":8.0,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Urea-assisted fabrication of defect and oxygen vacancy-enriched Ce(MoO4)2 oxidase mimetics: A dual-mode sensor array for detection and recognition of phenolic antioxidants 尿素辅助制造富含缺陷和氧空位的 Ce(MoO4)2 氧化酶模拟物:用于检测和识别酚类抗氧化剂的双模传感器阵列
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2024-11-09 DOI: 10.1016/j.snb.2024.136922
Jing-Jing Luo, Shu-Chang Zhao, Dan Wu, Ya-Nan Kong, Chang Liu, Ya-Jing Wang, Xun Zhu, Rui-Lin Liu
{"title":"Urea-assisted fabrication of defect and oxygen vacancy-enriched Ce(MoO4)2 oxidase mimetics: A dual-mode sensor array for detection and recognition of phenolic antioxidants","authors":"Jing-Jing Luo,&nbsp;Shu-Chang Zhao,&nbsp;Dan Wu,&nbsp;Ya-Nan Kong,&nbsp;Chang Liu,&nbsp;Ya-Jing Wang,&nbsp;Xun Zhu,&nbsp;Rui-Lin Liu","doi":"10.1016/j.snb.2024.136922","DOIUrl":"10.1016/j.snb.2024.136922","url":null,"abstract":"<div><div>Structurally similar phenolic compounds are involved in numerous biological processes and disease regulation, while the simultaneous determination and discrimination of them is still a great challenge. Herein, an oxygen vacancy and defect-enriched Ce(MoO<sub>4</sub>)<sub>2</sub> oxidase mimetics with tunable oxidase-like activity was successfully fabricated using a combined urea-assisted hydrothermal-annealing and peroxide etching-induced surface reconstruction strategy, and the as-prepared MoCe-based oxidase mimetics was constructed as a set of colorimetric/photothermal dual-mode triple-channel array sensors for simultaneously detecting and discriminating multiple phenolic compounds. Based on the different inhibition effects of phenolic compounds on 3,3’,5,5’-tetramethylbenzidine (TMB) oxidation, diverse colorimetric/photothermal signal variations are collected as \"fingerprints\" on the array sensors. The constructed colorimetric/photothermal array sensors can be successfully used to detect and recognize five phenolic compounds including gallic acid, quercetin, catechin, kaempferol and caffeic acid from sea buckthorn seeds. This dual-mode array sensors not only had the low determination limits for the five phenolic compounds and high anti-interference power, but also had better self-calibration and mutual verification characteristics than the single-mode detection. The current work not only endows an ingenious means to fabricate high catalytic active Ce(MoO<sub>4</sub>)<sub>2</sub> oxidase mimetics, but also exploits a high sensitive array sensor for the rapid detection and recognition of phenolic compounds from food samples.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136922"},"PeriodicalIF":8.0,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The preparation of SiO2/GO/PVA based hydrogel sensor and its application for rapid and sensitive detection of NH3 基于 SiO2/GO/PVA 的水凝胶传感器的制备及其在快速灵敏检测 NH3 中的应用
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2024-11-09 DOI: 10.1016/j.snb.2024.136885
Xueqin Du , Shuang Gu , Xuemei Wang , Shang Zhang , Bin Zhang , Guoming Yu , Zhenhe Wang , Wei Chen , Qiuhong Li
{"title":"The preparation of SiO2/GO/PVA based hydrogel sensor and its application for rapid and sensitive detection of NH3","authors":"Xueqin Du ,&nbsp;Shuang Gu ,&nbsp;Xuemei Wang ,&nbsp;Shang Zhang ,&nbsp;Bin Zhang ,&nbsp;Guoming Yu ,&nbsp;Zhenhe Wang ,&nbsp;Wei Chen ,&nbsp;Qiuhong Li","doi":"10.1016/j.snb.2024.136885","DOIUrl":"10.1016/j.snb.2024.136885","url":null,"abstract":"<div><div>Gas sensors have received significant interest due to their miniaturization, low power consumption and high reliability. In this paper, hydrogel film with high sensing properties toward NH<sub>3</sub> were prepared using silicon dioxide (SiO<sub>2</sub>), porous graphene oxide (GO) and polyvinyl alcohol (PVA). The morphology and structure of hydrogel were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectra (FT-IR) and specific surface area analysis. Compared with the conventional gas sensors based on planar GO sheets, the prepared porous SiO<sub>2</sub>/GO/PVA based hydrogel sensor could be used to detect NH<sub>3</sub> with low concentration (10 ppm) and wide range of 10–1000 ppm. At the same time, a high response rate of 118 % and an ultra-fast recovery (12 s) were achieved. Finally, a sensing mechanism for SiO<sub>2</sub>/GO/PVA based hydrogel film was proposed: NH<sub>3</sub> was adsorbed onto the surface of the film through hydrogen bonding and then reacted with the oxygen negative ions on the surface of the film to form nitrogen oxides. After degassing, oxygen was adsorbed on the surface of the film again to form oxygen negative ions. In addition, the film could monitor the freshness of fish over a period of 0–7 days, where the correlation between the resistance change and TVB-N was as high as 0.975. This work demonstrates the effectiveness of porous SiO<sub>2</sub>/GO/PVA based hydrogel film in improving gas-sensitive properties, providing a viable solution for food freshness detection, contamination tracking, and hazardous substances monitoring, etc.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136885"},"PeriodicalIF":8.0,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
One-pot, real-time monitoring and detection of chemical-producing capability in Escherichia coli using fluorophore-quencher-based aptasensor 利用基于荧光团-淬灭剂的灵敏传感器,对大肠杆菌的化学生产能力进行单锅实时监测和检测
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2024-11-08 DOI: 10.1016/j.snb.2024.136916
Kibeom Kim , Tae Yeong Jang , Sang Ho Kim, Su Min Oh, Seung Min Yoo
{"title":"One-pot, real-time monitoring and detection of chemical-producing capability in Escherichia coli using fluorophore-quencher-based aptasensor","authors":"Kibeom Kim ,&nbsp;Tae Yeong Jang ,&nbsp;Sang Ho Kim,&nbsp;Su Min Oh,&nbsp;Seung Min Yoo","doi":"10.1016/j.snb.2024.136916","DOIUrl":"10.1016/j.snb.2024.136916","url":null,"abstract":"<div><div>Microbial engineering for the production of valuable chemicals has garnered global attention as a promising solution to environmental issues associated with the oil industry. Recent advances in chemical-producing cells have focused on technologies that regulate multiple genes and create extensive cell libraries. Therefore, there is a critical need for monitoring and screening technologies that can rapidly analyse large cell libraries. Here, we demonstrated the repurposing of a fluorophore-quencher (F-Q) and aptamer-based sensing strategy as a cell screening tool for the development of microbial cell factories. The FAM-labelled capture strand and the BHQ1-modified short strand form a structure-switching double-stranded DNA construct, transitioning from a fluorescence ‘off’ to an ‘on’ state when bacterial cells produce the target chemical. To ensure effective adaptation, we conducted extensive system optimization across various culture conditions. As practical applications, we analysed an <em>E. coli</em> strain library engineered to produce L-phenylalanine, demonstrating high correlation with HPLC results in both small well plate and flask cultures, as well as in real-time monitoring. The system also effectively monitored caffeine-producing strains, highlighting its capability in detecting small quantities of chemicals. The system proved efficient for analysing microbial cells producing high-value chemicals.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136916"},"PeriodicalIF":8.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineering of a low intrinsic fluorescence and chemical-stable fluorescent probe enables highly sensitive detection of biothiols and high-fidelity imaging of dihydroartemisinin-induced ferroptosis 低本征荧光和化学稳定荧光探针的工程设计实现了生物硫醇的高灵敏度检测和双氢青蒿素诱导铁中毒的高保真成像
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2024-11-08 DOI: 10.1016/j.snb.2024.136913
Qiaozhen Ye , Tenglong Zou , Bolin Chen , Li Xu , Zhiyang Yuwen , Hongwen Liu , Kai Zhang
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