Sensors and Actuators B: Chemical最新文献_第9页

Enzymatic ablation of bimetallic Au@Ag nanocomposites enabling the smartphone-based colorimetric visualization of S1 nuclease

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-20 DOI: 10.1016/j.snb.2025.137487

Ziqing Qiu , Xiaojun Liu , Yanfei Zhang , Shu Zhu , Meiyun Chen , Yujie Zhao , Xingyu Luo

{"title":"Enzymatic ablation of bimetallic Au@Ag nanocomposites enabling the smartphone-based colorimetric visualization of S1 nuclease","authors":"Ziqing Qiu , Xiaojun Liu , Yanfei Zhang , Shu Zhu , Meiyun Chen , Yujie Zhao , Xingyu Luo","doi":"10.1016/j.snb.2025.137487","DOIUrl":"10.1016/j.snb.2025.137487","url":null,"abstract":"<div><div>S1 nuclease is an important endonuclease involved in multiple biological processes. It is essential to develop suitable detection methods to analyze the S1 nuclease activity for further understanding its biological functions. Although conventional methods for S1 nuclease detection have been well-designed including the gold nanoparticles (AuNPs)-related analytical methods. It still remains challenges in further improving their detection performances. To address these challenges, we report a novel smartphone-based colorimetric sensing method using the bimetallic Au@Ag nanocomposites (Au@Ag NCs) to realize the portable, sensitive, analytical instrument-free, and visual detection of S1 nuclease in real samples. In this method, the bare AuNPs was primarily modified with L1 DNA for supporting the silver (Ag) shell. With the formation of Ag shell, the prepared Au@Ag NCs display the enhanced localized surface plasma resonance (LSPR) effect by producing the characteristic UV–vis absorption peak of Ag (<em>Abs</em><sub>Ag</sub>), while the <em>Abs</em><sub>Au</sub>/<em>Abs</em><sub>Ag</sub> values will be promoted by the enzymatic ablation of Ag shell when L1 DNA was cleaved by S1 nuclease. Thus, Au@Ag NCs allow the colorimetric, sensitive and selective sensing toward S1 nuclease activity with the detection linear range varied from 0.1 to 50 U/mL and the detection limit as low as 0.02 U/mL. Furthermore, the colorimetric signals change also display the obvious color change in Au@Ag NCs and a smartphone-based sensing platform has been constructed to reveal the Red/Green/Blue (RGB) quantification and visual analysis of S1 nuclease in different scenarios. Therefore, this method will hold great potential in realizing the S1 nuclease detection with preferable detection performances.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"432 ","pages":"Article 137487"},"PeriodicalIF":8.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of NH3 impurities in H2 environment exploiting quartz-enhanced photoacoustic spectroscopy with an optimized spectrophone

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-20 DOI: 10.1016/j.snb.2025.137488

Chaofan Feng , Ruyue Cui , Giansergio Menduni , Andrea Zifarelli , Pietro Patimisco , Angelo Sampaolo , Vincenzo Spagnolo , Lei Dong , Hongpeng Wu

{"title":"Detection of NH3 impurities in H2 environment exploiting quartz-enhanced photoacoustic spectroscopy with an optimized spectrophone","authors":"Chaofan Feng , Ruyue Cui , Giansergio Menduni , Andrea Zifarelli , Pietro Patimisco , Angelo Sampaolo , Vincenzo Spagnolo , Lei Dong , Hongpeng Wu","doi":"10.1016/j.snb.2025.137488","DOIUrl":"10.1016/j.snb.2025.137488","url":null,"abstract":"<div><div>We report on a quartz-enhanced photoacoustic spectroscopy (QEPAS) sensor for ammonia impurities detection in hydrogen. A quantum cascade laser with a central emission wavelength of 9.062 μm was used to excite the NH<sub>3</sub> absorption line at 1103.44 cm<sup>−1</sup>, with a linestrength of 1.51ꞏ10<sup>−19</sup> cm/molecule. Compared to detecting contaminants in ambient air, the distinct properties of hydrogen required the design of a dedicated QEPAS spectrophone optimized for operation in hydrogen-based mixtures. The custom-made spectrophone was composed by a QTF excited at the first overtone mode at 44 kHz, acoustically coupled with an easy-to-align, dual-tube amplification system. The custom-made spectrophone was implemented in a QEPAS sensor, achieving a minimum detection limit of 95 ppb with a lock-in integration time of 0.1 s. Furthermore, the Allan-Werle deviation analysis returned a detection limit as low as 1.5 ppb when for an integration time of 30 s.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"432 ","pages":"Article 137488"},"PeriodicalIF":8.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143462269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-enhanced biosensor with luminophore-catalyst integrated strategies and ECL-RET for ultrasensitive SARS-CoV-2 N immunoassay

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-19 DOI: 10.1016/j.snb.2025.137478

Lingye Kong , Lu Zhao , Huixin Liang , Xiang Ren , Hongmin Ma , Xuejing Liu , Dawei Fan , Dan Wu , Qin Wei

{"title":"Self-enhanced biosensor with luminophore-catalyst integrated strategies and ECL-RET for ultrasensitive SARS-CoV-2 N immunoassay","authors":"Lingye Kong , Lu Zhao , Huixin Liang , Xiang Ren , Hongmin Ma , Xuejing Liu , Dawei Fan , Dan Wu , Qin Wei","doi":"10.1016/j.snb.2025.137478","DOIUrl":"10.1016/j.snb.2025.137478","url":null,"abstract":"<div><div>The large distance between luminophore and co-reactant accelerator leads to the increased contact time between luminophore and co-reactant radicals generated during the catalytic process. This disadvantage can affect electrochemiluminescence (ECL) efficiency of the constructed sensor. To solve this problem, a luminophore-catalyst integration strategy was proposed. Specifically, Cu<sup>2+</sup> was introduced in ZrEu-MOF, in which Cu<sup>2+</sup> with catalysis for K<sub>2</sub>S<sub>2</sub>O<sub>8</sub> endowed the composite ZrEuCu-MOF with self-catalysis function. Thus, ZrEuCu-MOF had the self-enhanced ECL characteristic. To enhance the sensitivity of the ECL sensor, the ECL resonance energy transfer (ECL-RET) strategy achieved a quenching effect. The luminophore ZrEuCu-MOF can occur efficient RET process to quencher YRF-Au, which resulted in obvious signal responses. Based on these strategies, the constructed ECL biosensor had a wide detection range of 1 fg/mL to 100 ng/mL and a low limit of detection (LOD) of 8.34 fg/mL, revealing a good sensitivity. The constructed ECL biosensor with these strategies opened a new horizon for efficient and highly sensitive SARS-C<sub>O</sub>V-2 N detection.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"432 ","pages":"Article 137478"},"PeriodicalIF":8.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time monitoring and multicolor visual analysis of hydrogen peroxide based on antenna effect-induced dual signal amplification strategy

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-19 DOI: 10.1016/j.snb.2025.137486

Lingyan Gui , Xu Teng , Ye Yang , Tao Liu , Yaru Liang , Jun Zeng , Xianying Zhang , Linhai Li

{"title":"Real-time monitoring and multicolor visual analysis of hydrogen peroxide based on antenna effect-induced dual signal amplification strategy","authors":"Lingyan Gui , Xu Teng , Ye Yang , Tao Liu , Yaru Liang , Jun Zeng , Xianying Zhang , Linhai Li","doi":"10.1016/j.snb.2025.137486","DOIUrl":"10.1016/j.snb.2025.137486","url":null,"abstract":"<div><div>Dual-emission fluorescence (FL) sensing platform could not only facilitate sensitive visual sensing, but also increase detection accuracy through the change of the FL response and FL color. Herein, a novel ratiometric FL sensing platform was fabricated using the dual-ligand-functionalized dual-emission Eu(III) nanomaterial (i.e., Eu-CDs) based on antenna effect-induced dual signal amplification strategy. In the as-prepared nanomaterial, 2-thenoyltrifluoro-acetone was used as the first ligand to sensitize the FL characteristic emission of Eu(III). Interestingly, the use of carbon dots as the second ligand for Eu(III) could not only further enhance the FL emission of Eu(III), but also serve as a reference signal to achieve the ratiometric FL signals for the detection of hydrogen peroxide. The detection mechanism was that the S elements in the thiophene unit could be turned into sulfone groups by oxidation with hydrogen peroxide, which changed the coordination environment of Eu(III), leading to the decrease in FL intensity at 616 nm. Based on the ratiometric FL signals, we realized the real-time monitoring and multicolor visual analysis of hydrogen peroxide with a detection limit of 1.88 μM. The extraordinary detection capability of hydrogen peroxide achieved the distinction of normal cells from cancer cells and the highly selective determination of serum glucose as the model target in hydrogen peroxide-involved enzymatic reaction. This work not only provided a novel path for the fabrication of high-performance ratiometric FL sensing platform by ligand-functionalized dual-emission Eu(III)-based nanomaterial, but also showed the potential feasibility for rational design of the FL sensing platform.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"432 ","pages":"Article 137486"},"PeriodicalIF":8.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultraviolet light activated ultrahigh frequency surface acoustic wave DMMP gas sensor with 10 ppb detection limit at room temperature

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-19 DOI: 10.1016/j.snb.2025.137452

Shengyu Wen , Long Li , Jian Zhou , Jinbo Zhang , Hui Chen , Yihao Guo , Yongqing Fu , Huigao Duan

{"title":"Ultraviolet light activated ultrahigh frequency surface acoustic wave DMMP gas sensor with 10 ppb detection limit at room temperature","authors":"Shengyu Wen , Long Li , Jian Zhou , Jinbo Zhang , Hui Chen , Yihao Guo , Yongqing Fu , Huigao Duan","doi":"10.1016/j.snb.2025.137452","DOIUrl":"10.1016/j.snb.2025.137452","url":null,"abstract":"<div><div>Dimethyl methylphosphonate (DMMP) is commonly used as a simulant for sarin in calibrating organophosphorus detectors. Among various DMMP sensors, surface acoustic wave (SAW) sensors are gaining significant attention for their attributes of wireless monitoring, digital outputs, and cost-effectiveness. However, there is a significant challenge remained as it is difficult to simultaneously improve sensitivity and reduce limit of detection (LOD) when monitoring the DMMP at room temperature. In this study, a comprehensive strategy was developed by applying Cu ion-doped ZrO<sub>2</sub> (Cu-ZrO<sub>2</sub>) nanocomposite as a high-performance sensitive layer coated onto an ultrahigh frequency (4.98 GHz) SAW sensor for detecting low concentration DMMP at room temperature under ultraviolet (UV) light activation. The developed ultrahigh frequency SAW DMMP sensor demonstrated an exceptional sensitivity of 1198.2 kHz/ppm, ∼1198 times higher than that of a conventional SAW sensor operated at a frequency of 162.4 MHz. It showed an ultralow LOD of 10 ppb, rapid response/recovery times of 39 s/25 s, and a long-term stability up to 30 days. Such the exceptional performance is primarily attributed to the Cu-ZrO<sub>2</sub> sensitive layer, of which the doped Cu ions lead to the formation of more unsaturated Zr<sup>4 +</sup> ions, increasing the Lewis acid sites and promoting the effective interactions between Lewis sites and DMMP molecules. In addition, attributed to the ultrahigh resonant frequency of the SAW device, a giant elastic loading effect was identified to significantly enhance the sensing performance. This study provides a promising and versatile methodology for achieving ultrahigh sensitivity DMMP detection based on the SAW technology.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"432 ","pages":"Article 137452"},"PeriodicalIF":8.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enabling ultrahigh loading of AIEgens through alkyne-amine click reactions for highly sensitive colorimetric-fluorescent dual-readout lateral flow immunoassay

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-19 DOI: 10.1016/j.snb.2025.137473

Yuankui Leng , Xiaolong Wang , Wenjing Li , Xiangkai Lin , Xiangmin Li , Xiaolin Huang

{"title":"Enabling ultrahigh loading of AIEgens through alkyne-amine click reactions for highly sensitive colorimetric-fluorescent dual-readout lateral flow immunoassay","authors":"Yuankui Leng , Xiaolong Wang , Wenjing Li , Xiangkai Lin , Xiangmin Li , Xiaolin Huang","doi":"10.1016/j.snb.2025.137473","DOIUrl":"10.1016/j.snb.2025.137473","url":null,"abstract":"<div><div>Colorimetric-fluorescent dual-readout lateral flow immunoassays are highly valued for their versatility in diverse applications and enhanced accuracy through the mutual validation of two signals. However, their sensitivity is typically limited by the signal intensity of current colorimetric-fluorescent probes. In this research, a novel colorimetric-fluorescent bifunctional nanoparticle was developed by densely packing aggregation-induced emission luminogens (AIEgens) with high molar extinction coefficients into dendritic mesoporous silica nanoparticles (DMSN) using the alkyne-amine click reaction. The DMSN demonstrated an ultrahigh loading capacity of AIEgens, reaching 1.36 mg of AIEgens per mg of DMSN. This synthesis strategy achieved high signal intensities in both colorimetric and fluorescent modes due to the dense loading of AIEgens. Subsequently, a metal-polyphenol network coating was applied to enhance hydrophilicity, prevent dye leakage, and facilitate rapid antibody immobilization through a straightforward mixing process. These bifunctional nanoparticles were then employed as nanoprobes in competitive and sandwich lateral flow immunoassays for the rapid detection of T2 toxin in chicken feed and <em>Staphylococcus aureus</em> enterotoxin A (SEA) in skim milk. The assays demonstrated high sensitivity with half inhibition concentrations of 0.62 ng/mL and 0.59 ng/mL for T2 toxin using colorimetric and fluorescent signals, respectively. Detection limits of 7.81 ng/mL and 0.43 ng/mL were achieved for SEA through colorimetric and fluorescent analysis, respectively.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"432 ","pages":"Article 137473"},"PeriodicalIF":8.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screen-printed PANI/MoS2-based flexible gas sensor for sub-ppm NH3 detection: Experimental and DFT investigations

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-19 DOI: 10.1016/j.snb.2025.137453

Aakanksha Jain , Ajithkumar M. Parambil , Siddhartha Panda

{"title":"Screen-printed PANI/MoS2-based flexible gas sensor for sub-ppm NH3 detection: Experimental and DFT investigations","authors":"Aakanksha Jain , Ajithkumar M. Parambil , Siddhartha Panda","doi":"10.1016/j.snb.2025.137453","DOIUrl":"10.1016/j.snb.2025.137453","url":null,"abstract":"<div><div>Detecting ammonia (NH<sub>3</sub>) in the sub-ppm range with high sensitivity and selectivity at room temperature is essential for non-invasive disease detection, environmental monitoring, and food quality control. Conducting polymers like polyaniline (PANI) are used in fabricating NH<sub>3</sub> sensors that function at room temperature, but they often exhibit low sensitivity. To address this, researchers have explored PANI-based composites. This study introduces a flexible NH<sub>3</sub> sensor based on a PANI/MoS<sub>2</sub> composite for sub-ppm detection at room temperature, focusing on the impact of composite morphology on sensing performance. Fabricated on a flexible PET substrate via screen printing, the sensor showed an 11 % improvement in response to 10 ppm NH<sub>3</sub> compared to pristine PANI, with a detection limit of 122 ppb and a response of 0.029. The sensor operates effectively at room temperature, making it suitable for wearable devices and demonstrated mechanical stability with a consistent response over nine months. Enhanced sensing is attributed to the p − n heterojunction, supported by Electrochemical Impedance Spectroscopy studies. Bader charge analysis and charge density difference revealed that incorporating MoS<sub>2</sub> with PANI enhanced the composite selectivity towards NH<sub>3</sub>. A prototype has been developed to demonstrate that this flexible, room temperature-based gas sensor can be utilized for real-time monitoring applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"432 ","pages":"Article 137453"},"PeriodicalIF":8.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a rapid, sequence-specific, and visual loop-mediated isothermal amplification (LAMP) assay leveraging a universal molecular beacon (uMB) during food safety analysis

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-19 DOI: 10.1016/j.snb.2025.137476

Ying Yang , Shihui Wang , Xiong Xiong , Tianlong Wang , Hongwei Song , Yan Guo , Libin Wang

{"title":"Development of a rapid, sequence-specific, and visual loop-mediated isothermal amplification (LAMP) assay leveraging a universal molecular beacon (uMB) during food safety analysis","authors":"Ying Yang , Shihui Wang , Xiong Xiong , Tianlong Wang , Hongwei Song , Yan Guo , Libin Wang","doi":"10.1016/j.snb.2025.137476","DOIUrl":"10.1016/j.snb.2025.137476","url":null,"abstract":"<div><div>Molecular beacon (MB) has demonstrated great potential for target-specific detection during loop-mediated isothermal amplification (LAMP), which, however, is constrained by the designing complexity and the great cost of target-dedicated probe screening. The present study aimed to establish a universal MB (uMB), and develop a novel sequence-specific LAMP assay based on the uMB probe for end-point visual inspection. Specifically, a novel uMB and a stem-loop primer complex (SPCX) were successfully designed, which maintained relatively high stability, avoiding unfolding for non-target specimen at the reaction temperature. Instead, SPCX could get open in the presence of target DNA, and afterwards rearranged to serve as the complementary analyte for uMB. The target-probe binding would trigger the conformational change of uMB, thus realizing end-point visual inspection. Under the optimal conditions, the novel uMB-LAMP assay can specifically detect mislabeled escolar and Salmonella contamination within 30 min in a close-tube format, with the detection limit of 1 pg DNA and 2 × 10<sup>1</sup> CFU/mL, respectively. When the target was changed, only the LAMP primers need to be re-designed and partial sequence of SPCX at the both ends needs to be substituted, demonstrating remarkable versatility and adaptability.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"432 ","pages":"Article 137476"},"PeriodicalIF":8.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric/electrochemical dual-mode aptasensor with phosphatase-like ceria nanozyme for fast and ultrasensitive detection of Ochratoxin A

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-19 DOI: 10.1016/j.snb.2025.137482

Jian Tang , Yulong Li , Yaochun Yao , Rong Hu

{"title":"Colorimetric/electrochemical dual-mode aptasensor with phosphatase-like ceria nanozyme for fast and ultrasensitive detection of Ochratoxin A","authors":"Jian Tang , Yulong Li , Yaochun Yao , Rong Hu","doi":"10.1016/j.snb.2025.137482","DOIUrl":"10.1016/j.snb.2025.137482","url":null,"abstract":"<div><div>Ochratoxin A (OTA) causes protein and DNA damage and is nephrotoxic, carcinogenic, genotoxic, and immunotoxic, and is recognized as a possible human carcinogen. In this study, we developed a dual-mode aptasensor combining both colorimetric and electrochemical methods for highly sensitive OTA detection using Ce-FMA with phosphatase-like activity. OTA aptamers are hybridized with linker probes to create polyvalent biotinylated scaffolds carrying multiple aptamers and Ce-FMA signal tags, greatly magnifying the signal. Due to the excellent phosphatase-like activity of the Ce-FMA nanozyme, it can catalyze the transformation of colorless p-nitrophenyl disodium phosphate (PNPP) to yellow-colored p-nitrophenol (PNP), enabling electrochemical and colorimetric dual-mode signal outputs, delivering more reliable outputs. Unlike traditional colorimetric assay, the presence of polyphenols, ascorbic acid, and reduced glutathione does not affect the performance. Moreover, the total assay time is reduced to 90 minutes. This dual-mode platform is simple, offering high sensitivity, reproducibility, and selectivity. The OTA detection range spans from 100 fg/mL to 150 ng/mL, with a detection limit of 26 fg/mL (3σ rule). Food samples, including corn and wheat, were analyzed, and the results aligned with those obtained from commercial enzyme-linked immunosorbent assays (ELISA) kits.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"432 ","pages":"Article 137482"},"PeriodicalIF":8.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acridone-mediated photo-driven in situ amplification for melanoma biomarkers biosensing

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-19 DOI: 10.1016/j.snb.2025.137479

Shuaibing Yu , Qinyuan Xu , Fenghong Zhang , Jinming Kong , Xueji Zhang

{"title":"Acridone-mediated photo-driven in situ amplification for melanoma biomarkers biosensing","authors":"Shuaibing Yu , Qinyuan Xu , Fenghong Zhang , Jinming Kong , Xueji Zhang","doi":"10.1016/j.snb.2025.137479","DOIUrl":"10.1016/j.snb.2025.137479","url":null,"abstract":"<div><div>Melanoma is a malignant tumor caused by abnormal expression of melanin, primarily on the surface of the skin. The overexpression of tyrosinase, a rate-limiting enzyme of melanin synthesis, results in the overproduction of melanin. Therefore, quantitative tyrosinase assay is crucial for prevention and therapy of patients at early melanoma. Herein, an electrochemical biosensor for low abundance tyrosinase detection was developed using photoinduced atom transfer radical polymerization (photo-ATRP) driven by 10-phenyl-9(10 H)-acridone (low excited state potential and long triplet excited state lifetime). Briefly, the indium tin oxide (ITO) electrode was used as a carrier, and a tyrosinase substrate probe was immobilized on electrode surface via silanization. In the presence of tyrosinase, monophenolic hydroxyl group of the probe is catalyzed to bisphenolic hydroxyl, which introduces an initiator with a boronic acid group. Subsequently, the photo-ATRP was driven under UV irradiation, leading to the polymerization of electrochemically active monomers and signal amplification. Compared to traditional nanomaterial and enzyme-based strategies, acridone-mediated photo-ATRP signal amplification offers significant advantages of simplicity, speed, and cost-effectiveness. The limit of detection (LOD) for tyrosinase using the signal amplification biosensing platform is 0.048 U/L. Furthermore, the biosensor exhibits excellent selectivity and immunity to interference, and has been successfully implemented for the detection of real samples, thereby indicating that it has significant prospective for applications in the timely prevention and diagnosis of melanoma.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"432 ","pages":"Article 137479"},"PeriodicalIF":8.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0