A near-infrared fluorescent probe for the detection of vicinal-dithiol-containing proteins and its application in vivo

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-07-01 DOI:10.1016/j.snb.2025.138235

Guangwei Wang, Fang-Fang Guo, Jiaojiao Wu, Liang-Liang Gao, Xinrong Tian, Jianjian Zhang, Yuan Guo

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Abstract

Vicinal-dithiol-containing proteins (VDPs) play a significant role in maintaining cellular redox homeostasis and serve as important biomarkers for diagnosing redox-related diseases, such as obesity and cancer. However, the currently reported fluorescent probes are restricted in biological applications due to their short emission wavelengths and insufficient sensitivity. To address this limitation, we developed a novel near-infrared fluorescent probe (M1) for sensitive tracking of VDPs by utilizing coumarin-benzopyrylium moiety as the fluorophore and two methoxy-substituted maleimide groups matching double active cysteine residues of VDPs as the recognition site. M1 exhibits negligible fluorescence in aqueous media but emits strong red fluorescence upon specific binding to VDPs. The probe shows high sensitivity for detecting reduced bovine serum albumin (rBSA) within the concentration range of 0-7.5 μM, with a detection limit as low as 0.070 μM. Furthermore, M1 has been successfully applied not only to super-resolution fluorescence imaging of VDPs in A549 cells, but also to confocal fluorescence imaging of VDPs in zebrafish.

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一种近红外荧光探针检测邻苯二硫醇蛋白及其在体内的应用

邻近二硫醇含蛋白（vdp）在维持细胞氧化还原稳态中发挥重要作用，并作为诊断氧化还原相关疾病（如肥胖和癌症）的重要生物标志物。然而，目前报道的荧光探针由于其发射波长短，灵敏度不足，在生物学上的应用受到限制。为了解决这一限制，我们开发了一种新的近红外荧光探针（M1），用于灵敏跟踪vdp，利用香豆素-苯并吡啶片段作为荧光基团，两个甲氧基取代的马来酰亚胺基团匹配vdp的双活性半胱氨酸残基作为识别位点。M1在水介质中表现出可忽略的荧光，但在与vdp特异性结合时发出强烈的红色荧光。该探针对还原牛血清白蛋白（rBSA）的检测灵敏度在0 ~ 7.5 μM范围内，检测限低至0.070 μM。此外，M1不仅成功应用于A549细胞vdp的超分辨荧光成像，还成功应用于斑马鱼vdp的共聚焦荧光成像。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.