Sensors and Actuators B: Chemical最新文献_第8页

Tin oxide nanoparticles anchored on ordered mesoporous carbon for efficient acetone sensing 锚定在有序介孔碳上的氧化锡纳米粒子用于高效丙酮传感

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-16 DOI: 10.1016/j.snb.2024.136957

Jian Fang , Wei Wang , Yizhuo Fan , Heng Guan , Qilin Wang , Dali Liu , Ruiliang Xu , Shengping Ruan

{"title":"Tin oxide nanoparticles anchored on ordered mesoporous carbon for efficient acetone sensing","authors":"Jian Fang , Wei Wang , Yizhuo Fan , Heng Guan , Qilin Wang , Dali Liu , Ruiliang Xu , Shengping Ruan","doi":"10.1016/j.snb.2024.136957","DOIUrl":"10.1016/j.snb.2024.136957","url":null,"abstract":"<div><div>Rationally designed combinations of semiconductor metal oxides (SMO) and carbon materials can lead to the development of sensing materials with excellent gas performance. Herein, SnO<sub>2</sub> nanoparticles (NPs) decorated on ordered mesoporous carbon (CMK-3) nanorods were synthetized by solvothermal and high-temperature calcination methods. Various characterization and gas sensing tests indicated that the resulting one-dimensional (1D) self-assembled SnO<sub>2</sub>@CMK-3 composite had a large specific surface area (88.02 m<sup>2</sup>/g) and excellent gas sensing performance. Specifically, at optimal working temperature, the as-prepared SnO<sub>2</sub>@CMK-3 sensor had a high response (122.1), low limit of detection, good linear fitting (R<sup>2</sup> = 0.9892), rapid response/recovery time (5/27 s) towards 50 ppm acetone. Meanwhile, by detecting six gases, including acetone, ammonia, formaldehyde, xylene, triethylamine, and ethanol, it was found that the SnO<sub>2</sub>@CMK-3 sensor showed good selectivity to acetone. The unique nanostructure, large specific surface area, high oxygen vacancy content, and the formation of heterojunctions accounted for the good performance to acetone. These results highlighted the potential application of the SnO<sub>2</sub>@CMK-3 composite for the detection of acetone gas and presented a promising approach to prepare SMO@carbon composites for VOCs detection.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"425 ","pages":"Article 136957"},"PeriodicalIF":8.0,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A superoxide anion activatable 7-Azaindole derived photoacoustic probe for high-contrast imaging of drug-induced liver injury 一种可被超氧阴离子激活的 7-氮杂吲哚衍生光声探针，用于药物性肝损伤的高对比度成像

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-15 DOI: 10.1016/j.snb.2024.136953

Xianjun Liu , Yu Yang , Lan Peng , Xiang Li , Fang-Yuan Qi , Guiting Zheng , Liangling Tan , Lei Qiao , Chong-Hua Zhang

{"title":"A superoxide anion activatable 7-Azaindole derived photoacoustic probe for high-contrast imaging of drug-induced liver injury","authors":"Xianjun Liu , Yu Yang , Lan Peng , Xiang Li , Fang-Yuan Qi , Guiting Zheng , Liangling Tan , Lei Qiao , Chong-Hua Zhang","doi":"10.1016/j.snb.2024.136953","DOIUrl":"10.1016/j.snb.2024.136953","url":null,"abstract":"<div><div>The accurate diagnosis of Drug-induced liver injury (DILI) plays essential role for the effective treatment and avoiding the worsening of liver injury. Considering superoxide anion (O<sub>2</sub><sup>•-</sup>) is closely associated with the progression of DILI, molecular probes that achieve high-contrast photoacoustic (PA) imaging of O<sub>2</sub><sup>•-</sup> in deeper tissues with high spatial resolution are valuable tools for the accurate diagnosis of DILI. However, the existing PA probes for O<sub>2</sub><sup>•-</sup> display the relatively short absorption wavelength and small bathochromic shift, limiting their PA activation folds. Here we construct a new O<sub>2</sub><sup>•-</sup> activatable 7-Azaindole derived probe (AIH-OTF) for PA imaging of DILI in vivo. A novel 7-Azaindole modified hemicyanine dye (AIH-OH) is incorporated as the PA reporter of AIH-OTF due to its long absorption wavelength, large molar extinction coﬃcient and low fluorescence quantum yield. It is demonstrated that AIH-OTF exhibits the highly sensitive and selective PA response to O<sub>2</sub><sup>•-</sup> with a large bathochromic shift (203 nm) and high activation fold (9.7 times). Cellular studies indicate that AIH-OTF can realize high-contrast PA imaging of the dynamic changes of endogenous O<sub>2</sub><sup>•-</sup> in living cells. AIH-OTF is successfully applied to detect the occurrence of drug-induced liver injury and assess the therapeutic efficacy through its PA response to O<sub>2</sub><sup>•-</sup>. We expect that AIH-OTF may serve as a useful imaging tool for the diagnosis and pathogenesis research of DILI.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"425 ","pages":"Article 136953"},"PeriodicalIF":8.0,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An ultrasensitive lysosome-targeting NIR fluorescence probe for detection of hydroxyl radical during ferroptosis and cuproptosis 一种超灵敏的溶酶体靶向近红外荧光探针，用于检测铁突变和铜突变过程中的羟基自由基

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-15 DOI: 10.1016/j.snb.2024.136951

Xue Luo , Qihao Rao , Shuangshuang Wei , Jiajia Lv , Yumei Wu , Mingyan Yang , Junjun Luo , Jie Gao , Xinmin Li , Zeli Yuan , Hongyu Li

{"title":"An ultrasensitive lysosome-targeting NIR fluorescence probe for detection of hydroxyl radical during ferroptosis and cuproptosis","authors":"Xue Luo , Qihao Rao , Shuangshuang Wei , Jiajia Lv , Yumei Wu , Mingyan Yang , Junjun Luo , Jie Gao , Xinmin Li , Zeli Yuan , Hongyu Li","doi":"10.1016/j.snb.2024.136951","DOIUrl":"10.1016/j.snb.2024.136951","url":null,"abstract":"<div><div>Hydroxyl radical (•OH) is one of the most destructive reactive oxygen species in biosystems, mainly produced by the participation of transition metals. Lysosomes, the major metabolic center of intracellular metals, may accelerate the generation of •OH by metal-participated reactions due to their acidic microenvironment, which may make lysosomes a major intracellular site for •OH production. In this work, an ultrasensitive NIR lysosome-targeting •OH fluorescence probe, Lyso-OH, was developed for <em>in situ</em> detection of •OH in lysosomes. The reaction of Lyso-OH and •OH leads to a large spectroscopic red-shift and a significant NIR fluorescence response at 655 nm. Such large changes in π-conjugation and spectroscopic properties can achieve an extremely low background fluorescence, which is helpful to obtain a high-contrast and ultrasensitive NIR fluorescence response, enabling Lyso-OH to detect the trace •OH produced by autoxidation of low-valent metals (e.g. Fe<sup>2+</sup> and Cu<sup>+</sup>). Lyso-OH has good lysosome-targeting ability and has been used for the fluorescence imaging of two transition metal-dependent regulated cell death processes, ferroptosis and cuproptosis. The results revealed that both ferroptosis and cuproptosis were accompanied by lysosomal •OH level increase. Moreover, Lyso-OH was also capable of monitoring the •OH level fluctuations in mice models of inflammation and tumor. The good analytical performance of Lyso-OH may allow it to be widely used in more •OH-associated physiological and pathological processes.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136951"},"PeriodicalIF":8.0,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scalable balloon catheter assisted contact enhancement of 3D electrode array for colon electrophysiological recording 用于结肠电生理记录的可扩展球囊导管辅助三维电极阵列接触增强技术

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-15 DOI: 10.1016/j.snb.2024.136955

Jiajin Xue , Chunlian Qin , Duote Cai , Shuhao Zhang , Xiu Yu , Jianliang Xiao , Zhigang Gao , Ning Hu , Haitao Liu

{"title":"Scalable balloon catheter assisted contact enhancement of 3D electrode array for colon electrophysiological recording","authors":"Jiajin Xue , Chunlian Qin , Duote Cai , Shuhao Zhang , Xiu Yu , Jianliang Xiao , Zhigang Gao , Ning Hu , Haitao Liu","doi":"10.1016/j.snb.2024.136955","DOIUrl":"10.1016/j.snb.2024.136955","url":null,"abstract":"<div><div>The clinical diagnosis of functional gastrointestinal disorders primarily relies on symptom assessment and physical examination, methods that are inherently limited due to undetermined specific biomarkers and susceptibility to subjective judgment. Electrophysiological techniques offer a clinically practical approach by precisely recording the electrical activities of the intestines, providing deeper biophysical insights. However, in vivo electrophysiological investigation of the intestines remains challenging, such as the stability of the electrode-tissue interface, high-frequency signal transmission, motion artifact, and signal interpretation. This study developed an endoscopic-based high spatiotemporal electrophysiological recording device, by integrating balloon catheters and flexible three-dimensional electrode arrays with a mapping function. We integrated the 3D electrode array on a scalable balloon catheter and deployed this device in rabbits, achieving a stable collection of multiphasic electrophysiological signals from the intestinal mucosa. In vivo electrophysiological recordings identified observable spike bursts associated with intestinal motility, as well as coordinated periodic pulses potentially related to intestinal pacemaker activities, revealing their activation modulation patterns. Further, longer-duration non-periodic spike bursts were also observed to link to spontaneous non-periodic peristaltic contractions. This technique realized through minimally invasive procedures, achieves a high spatiotemporal resolution in electrophysiological recording, providing a powerful tool for the diagnosis and research of functional bowel diseases.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136955"},"PeriodicalIF":8.0,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increasing the sensitivity of an amperometric, YSZ-based total-NOx sensor by opening the gas diffusion pathways in a porous La0.8Sr0.2MnO3 electrode 通过打开多孔 La0.8Sr0.2MnO3 电极中的气体扩散通道，提高基于 YSZ 的安培总氮氧化物传感器的灵敏度

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-15 DOI: 10.1016/j.snb.2024.136949

Xuebin Li , Jie Zou , Zhenqian Cheng , Weifeng Zhang , Xiaofeng Ye , Xie Meng , Xiaoqing Jiang , Dongxing Zhang , Jie Wang , Qinghui Jin , Jiawen Jian , Xin Zhang

{"title":"Increasing the sensitivity of an amperometric, YSZ-based total-NOx sensor by opening the gas diffusion pathways in a porous La0.8Sr0.2MnO3 electrode","authors":"Xuebin Li , Jie Zou , Zhenqian Cheng , Weifeng Zhang , Xiaofeng Ye , Xie Meng , Xiaoqing Jiang , Dongxing Zhang , Jie Wang , Qinghui Jin , Jiawen Jian , Xin Zhang","doi":"10.1016/j.snb.2024.136949","DOIUrl":"10.1016/j.snb.2024.136949","url":null,"abstract":"<div><div>To boost the sensitivity of amperometric total-NO<sub>x</sub> sensors, polymethyl methacrylate (PMMA) was employed as a pore former to open up the diffusion pathways of a porous La<sub>0.8</sub>Sr<sub>0.2</sub>MnO<sub>3</sub> (LSM) sensing electrode (SE). The porous LSM was characterized through scanning electron microscopy and mercury intrusion porosimetry, and its sensing performance was evaluated in a test setup based on a dynamic gas mixer. The results indicate that adding PMMA increases the gas diffusion pathway and the porosity of SE. Specifically, the NO<sub>x</sub> sensitivity increased from 4.794 to 6.516 μA/decade, then decreased to 2.428 μA/decade, with the increase in the SE porosity. These results suggest that high SE porosity benefits gas diffusion but diminishes the three-phase boundary for electrochemical reactions. Meanwhile, the sensor exhibited remarkable selectivity and consistency, which means that opening the gas diffusion pathway using PMMA is a reliable technology for high-temperature NO<sub>x</sub> sensors.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136949"},"PeriodicalIF":8.0,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequential separation and profiling of extracellular vesicles using antibody-aptamer conjugates 利用抗体-aptamer共轭物依次分离和分析细胞外囊泡

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-14 DOI: 10.1016/j.snb.2024.136939

D. Brambilla , F. Panico , M.S. Ünlü , M. Chiari

{"title":"Sequential separation and profiling of extracellular vesicles using antibody-aptamer conjugates","authors":"D. Brambilla , F. Panico , M.S. Ünlü , M. Chiari","doi":"10.1016/j.snb.2024.136939","DOIUrl":"10.1016/j.snb.2024.136939","url":null,"abstract":"<div><div>Extracellular vesicles (EVs) are membrane-bound vesicles secreted by cells, exhibiting diverse compositions reflective of their cellular origin. With significant potential as biomarkers for liquid biopsies, EV research has led to various isolation techniques. However, a consensus on the optimal strategy remains elusive. Immunoprecipitation, selectively capturing EVs based on surface markers, is promising but hindered by cost, low yields, and potential damage during release. In this study, we propose an innovative Antibody-Aptamer Conjugate (AAC): a three-component separation reagent for the separation of EVs. Combining an EV-specific antibody, a streptavidin-binding aptamer, and a unique barcode DNA sequence, this conjugate serves dual roles, facilitating both EV separation and subsequent multiplexed analysis. We detail the development and validation of the AAC, demonstrating its efficacy in isolating intact EVs from complex samples. The unique barcode DNA sequence enables high-throughput analysis on a DNA microarray chip, addressing limitations of existing methodologies. This approach offers a valid and cost-effective alternative for selective EV isolation and analysis, with implications for diagnostic and therapeutic advancements in liquid biopsy applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"424 ","pages":"Article 136939"},"PeriodicalIF":8.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effect of NiFe2O4 and MWCNTs for ppb-level acetone detection at 150 °C NiFe2O4 和 MWCNTs 在 150 oC 下检测ppb 级丙酮的协同效应

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-14 DOI: 10.1016/j.snb.2024.136948

Qiang Fu , Jiaheng Li , Stephan Handschuh-Wang , Xuechang Zhou , Yizhen Liu

{"title":"Synergistic effect of NiFe2O4 and MWCNTs for ppb-level acetone detection at 150 °C","authors":"Qiang Fu , Jiaheng Li , Stephan Handschuh-Wang , Xuechang Zhou , Yizhen Liu","doi":"10.1016/j.snb.2024.136948","DOIUrl":"10.1016/j.snb.2024.136948","url":null,"abstract":"<div><div>Acetone is a biomarker used in illness diagnosis and can lead to health issues. Chemiresistive sensors for acetone detection have been made using metal oxides, however they still require improvement in terms of sensitivity and operating temperature. In this study, we use a simple hydrothermal technique under surfactantless condition without subsequent high-temperature calcination treatment, which is more environment-friendly, energy-saving, to produce a variety of NiFe<sub>2</sub>O<sub>4</sub>/multi-walled carbon nanotubes (MWCNTs) composites with varying MWCNT contents for the detection of acetone. The findings demonstrate that the NiFe<sub>2</sub>O<sub>4</sub> /MWCNTs composite-based chemiresistive gas sensor possesses a strong performance of 53.4 (The ratio of the resistance of the sensor in acetone atmosphere to that of the sensor in air) to 100 ppm acetone at 150 °C, while a ppb-level detection limit (102 ppb), wide detection range and good sensitivity are achieved at 150 °C. The synergistic impact of MWCNTs and NiFe<sub>2</sub>O<sub>4</sub>, as well as the high porosity and large specific surface area of the composite contribute to the composite-based sensor's better sensing capability. Our findings suggest that the binary metal oxide (metal oxide containing two types of metallic elements) /carbon material composite might be useful in the development of stable and sensitive acetone sensors for application in both industry and healthcare.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"425 ","pages":"Article 136948"},"PeriodicalIF":8.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142637535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MoS2-mediated gap-mode surface plasmon enhancement: Construction of SPR biosensor for direct detection of LECT2 MoS2- 介导的间隙模式表面等离子体增强：构建用于直接检测 LECT2 的 SPR 生物传感器

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-14 DOI: 10.1016/j.snb.2024.136938

Yangyang Zhou , Hezhen Liu , Weiwen Zhang , Jingyao Wu , Kwangnak Koh , Hongxia Chen

{"title":"MoS2-mediated gap-mode surface plasmon enhancement: Construction of SPR biosensor for direct detection of LECT2","authors":"Yangyang Zhou , Hezhen Liu , Weiwen Zhang , Jingyao Wu , Kwangnak Koh , Hongxia Chen","doi":"10.1016/j.snb.2024.136938","DOIUrl":"10.1016/j.snb.2024.136938","url":null,"abstract":"<div><div>Serum levels of leukocyte cell-derived chemotaxin-2 (LECT2) have emerged as promising diagnostic biomarker for liver fibrosis. Targeting the LECT2/Tie1 signaling pathway holds potential for innovative treatment approaches to this condition. Despite this, the development of highly sensitive assays for serum LECT2 remains limited. Surface plasmon resonance (SPR), a label-free technique for detecting biomolecular interactions via refractive index (RI) changes, has demonstrated notable sensitivity for biomarker assays, though improving its sensitivity for direct LECT2 detection faces certain challenges. In this study, we present an innovative enhanced SPR biosensor designed to overcome current limitations in LECT2 detection. By incorporating molybdenum disulfide (MoS<sub>2</sub>) nanosheets as nano-spacer between the gold film and cyclodextrin functionalized-gold nanoparticles (CD-AuNPs), this gap-mode design significantly enhances the electric field intensity near the gold film surface, which effectively improves the detection sensitivity of the RI changes. Compared to traditional gold-film-only SPR sensors, our sensor achieves a remarkable sensitivity increase from 73.1 to 133.3 deg/RIU, with a commendable quality factor and detection accuracy. By further modifying the specific peptide of the terminal label phenylalanine (TFF) through the host-guest recognition effect of the CD hydrophobic cavity, we achieved a broad linear range and a low limit of detection (LOD = 0.6206 ng/mL) for the detection of the liver fibrosis marker LECT2. Our proposed enhanced SPR sensing platform demonstrates significant potential for clinical applications in LECT2 detection.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"425 ","pages":"Article 136938"},"PeriodicalIF":8.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RT-RAA with a lateral flow assay readout based on ssDNA hybridization for detection of RNA viruses – the case of SARS-CoV-2 基于ssDNA杂交的RT-RAA横向流动检测读数用于检测RNA病毒--以SARS-CoV-2为例

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-14 DOI: 10.1016/j.snb.2024.136864

João M. Vindeirinho, Eva Pinho, Licínia Gomes, Raquel Guiomar, Rea Maja Kobialka, Ahmed Abd El Wahed, Nuno F. Azevedo, Carina Almeida

{"title":"RT-RAA with a lateral flow assay readout based on ssDNA hybridization for detection of RNA viruses – the case of SARS-CoV-2","authors":"João M. Vindeirinho, Eva Pinho, Licínia Gomes, Raquel Guiomar, Rea Maja Kobialka, Ahmed Abd El Wahed, Nuno F. Azevedo, Carina Almeida","doi":"10.1016/j.snb.2024.136864","DOIUrl":"https://doi.org/10.1016/j.snb.2024.136864","url":null,"abstract":"The conjugation of Reverse-Transcription Recombinase-Aided Amplification (RT-RAA) and Nucleic Acid Lateral Flow Assays (NALFAs) is a useful strategy for the point-of-care detection of viruses like SARS-CoV-2. In this work we developed an end-point diagnostic method combining RT-RAA and NALFA that is based on the hybridization of ssDNA and relies exclusively on the interaction of nucleic acids for producing a specific signal.Different tails were used to modify a previously published set of primers targeting the RdRp gene, enabling the test of distinct sequences and lengths in terms of amplification efficiency. Additionally, RT-RAA amplification conditions, including the temperature and time of reaction, were optimized. Lateral flow strips were assembled and running conditions were adjusted. The best combination of tailed primers was selected and the resulting amplicons were successfully detected in the test strip. The developed RT-RAA-NALFA method showed a limit of detection (LOD) of ~12 RNA copies/µL and no cross-reactivity was seen testing the DNA/RNA of 16 different pathogens except for other SARS-CoV species, thus exhibiting good analytical sensitivity and specificity. The blind test of 50 clinical samples by RT-RAA-NALFA, showed a high agreement (18/21 positives and 28/29 negatives were correctly identified) with real-time RT-PCR results. These results lead to an overall clinical sensitivity of ~86% and an overall clinical specificity of ~97%. The clinical sensitivity value increases to ~94% for viral loads corresponding to a quantification cycle (Cq) below 30 in real-time RT-PCR. This emphasizes the suitability of the test for the efficient detection of RNA virus like SARS-CoV-2.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"98 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UV-activated TiO2/SnO2 oxygen sensor with improved response speed for low temperature and air-atmospheric operation 紫外线激活的 TiO2/SnO2 氧气传感器，响应速度更快，适用于低温和空气-大气操作

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2024-11-14 DOI: 10.1016/j.snb.2024.136947

Ruichen Shang , Hui Huang , Chenyang Liu , Mengyuan Wang

引用次数: 0