Sensors and Actuators B: Chemical最新文献

{"title":"Innovative bubble-enrichment and dual-mode POCT system for sensitive monitoring of chlorpyrifos bioaccumulation in complex matrices","authors":"Kejun Li ,&nbsp;Lianyuan Tang ,&nbsp;Yuanjiao Yang ,&nbsp;Yuemei Chen ,&nbsp;Chunhui Wu ,&nbsp;Xin Yuan ,&nbsp;Mei Zhang ,&nbsp;Ke Huang","doi":"10.1016/j.snb.2025.138030","DOIUrl":"10.1016/j.snb.2025.138030","url":null,"abstract":"<div><div>Chlorpyrifos (CPF), a widely used pesticide, poses significant health risks due to the bioaccumulation, necessitating effective on-site residue detection. Here, a portable online device integrating bubble enrichment and point-of-care testing (POCT) was developed for rapid on-site monitoring of CPF in mint plants. A bubble-bursting enrichment system was employed to extract trace CPF in complex matrices, followed by a dual-mode colorimetric/photothermal (PT) analysis platform based on enzyme-nanozyme cascade reactions. Platinum nanoparticles (PtNPs), exhibiting significant oxidase-like activity, catalyzed the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) to blue oxTMB with the help of singlet oxygen ¹O₂ in the absence of H₂O₂. The oxidation product, oxTMB, serves as a dual-mode signal reporter by simultaneously generating both colorimetric and PT signals. In the sensing strategy, butyrylcholinesterase (BChE) and acetylthiocholine (ATCh) were employed to produce thiocholine (TCh), which inhibited the formation of oxTMB by modulating the dispersity of PtNPs and reducing oxTMB production. CPF inhibited BChE activity, resulting in increase of two signals. The platform achieved limits of detection (LODs) of 0.052 ng mL⁻¹ and 5.3 ng mL⁻¹ for colorimetric and PT analysis, respectively, with strong resistance to interference. CPF levels in mint declined over a week post-spraying, consistent with expected degradation trends. The developed dual-mode platform demonstrated reliable on-site analytical performance for monitoring CPF residues in mint samples, providing a sensitive, portable, and robust approach for agricultural and environmental applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 138030"},"PeriodicalIF":8.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistically integrated WS2-MoSe2 nanowires cosputtered over porous silicon towards ppb level NO2 detection","authors":"Sonika Kodan ,&nbsp;Ashwani Kumar ,&nbsp;Amit Sanger ,&nbsp;Sheetal Issar ,&nbsp;V.K. Malik ,&nbsp;Ramesh Chandra","doi":"10.1016/j.snb.2025.138021","DOIUrl":"10.1016/j.snb.2025.138021","url":null,"abstract":"<div><div>Two-dimensional (2D) transition metal dichalcogenides (TMDs) have sparked significant research interest in gas sensing technologies owing to their large specific surface area, excellent sensitivity, high power efficiency, and ability to function at room temperature (RT). The present investigation outlines a highly selective and fully recoverable RT nitrogen dioxide (NO₂) gas sensor based on molybdenum diselenide (MoSe₂) and tungsten disulfide (WS₂) nanocomposite co-sputtered directly over electrochemically anodized porous silicon substrate (PSi) employing the DC magnetron sputtering technique. Here, the PSi substrate substantially boosts the sensor’s characteristics by providing a large surface area for the growth of nanostructured sensing film, facilitating numerous active sites for the target gas interaction. In addition, the PSi structure makes the sensor’s surface highly hydrophobic, making it an ideal candidate for sensing applications in harsh and humid atmospheres. Compared to their pristine counterparts (MoSe₂/PSi and WS₂/PSi), the hybrid WS₂-MoSe₂/PSi nanostructured sensor demonstrates a superior sensor response of ∼34.14 % with a fast response/recovery time (∼17.67 s/∼41.05 s) towards 50 ppb NO₂ gas at RT, highlighting the enhanced synergistic effects within the nanocomposite sensor. The proposed WS₂-MoSe₂/PSi gas sensor delivers exceptional selectivity and commendable stability for over 100 days with retention of ∼90.9 % towards 50 ppb NO₂ gas, even at RT (27˚C). Moreover, the sensor retains ∼92.44 % of its original response towards 50 ppb NO₂ under high relative humidity (∼80 % RH). Therefore, the remarkable sensing properties exhibited by the developed WS₂-MoSe₂/PSi sensor render enticing possibilities for developing next-generation room temperature NO₂ gas sensors.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 138021"},"PeriodicalIF":8.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dioleamide as a novel chemiluminescence reagent: Simple synthesis and highly sensitive bioenzyme detection","authors":"Xiangyu Cai,&nbsp;Yuying Jia,&nbsp;Xuepeng Wang,&nbsp;Hongyan Shan,&nbsp;Yanfu Huan","doi":"10.1016/j.snb.2025.138022","DOIUrl":"10.1016/j.snb.2025.138022","url":null,"abstract":"<div><div>In this work, a novel chemiluminescence (CL) reagent dioleamide (DOA) was synthesized, which contains a long chain organic compound with an amide as the core functional group. It exhibited stable and strong CL emission when sodium hypochlorite was used as the oxidant. A mechanistic study indicates that CL is mainly due to the process in which the reactive oxygen species (ROS) produced by the system during the chemical reaction oxidize the DOA to the excited state and then return to the ground state to release energy in the form of light radiation. Alkaline phosphatase (ALP) catalyzes the hydrolysis reaction of p-nitrophenyl phosphate (PNPP) to generate p-nitrophenol (PNP). The maximum absorption wavelength of PNP significantly overlaps with the CL emission wavelength of the DOA, enabling energy transfer from DOA to PNP and thus the CL intensity is weakened. Consequently, a simple, efficient, and sensitive ALP sensor based on chemiluminescence resonance energy transfer (CRET) system was developed with a detection limit of 0.020 U/L. The NaClO-DOA-PNP sensing system, which demonstrated a highly sensitive reaction to ALP in the range of 0.1–30 U/L, was successfully applied to the detection of ALP in fetal bovine serum.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 138022"},"PeriodicalIF":8.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long wavelength AIE enzyme prodrug-photosensitizer with dual cationic structure for fluorescence imaging of tumor cells and synergistic photodynamic therapy","authors":"Pengju Sun ,&nbsp;Guojie Li ,&nbsp;Yuanyuan Li ,&nbsp;Jialu Yang ,&nbsp;Ting Chen ,&nbsp;Yibing Wang ,&nbsp;Yanmei Zhou","doi":"10.1016/j.snb.2025.138031","DOIUrl":"10.1016/j.snb.2025.138031","url":null,"abstract":"<div><div>Enzyme Prodrug Therapy (EPT) represents a promising antitumor strategy to circumvent adverse reactions associated with chemotherapy. However, the current antitumor efficacy of EPT is constrained by its relatively limited therapeutic approach and the number of enzyme. Herein, we presented an esterase-activated AIE enzyme prodrug-photosensitizer <strong>TPN-Cb</strong> with an emission wavelength of 825 nm, characterized by its unique dual cationic structure that facilitated precise targeting of tumor cells. Upon cellular internalization, the precise cleavage of ester bonds by esterases enabled the dual release of the photosensitizer TPN-OH and the chemotherapeutic drug chlorambucil (CLB). TPN-OH accumulated in lysosomes and laser irradiation generated large amounts of O₂^-• for photodynamic therapy (PDT). CLB could be released into the nucleus causing damage was used in the EPT process. The synergistic effect of PDT and EPT induced lysosomal and nuclear dual organelle damage and significantly promoted the apoptosis of tumor cells. Furthermore, the large Stokes shift exhibited by TPN-OH equips it with exceptional bioimaging capabilities. This synergistic strategy of near-infrared fluorescence imaging technology and precision-targeted dual therapy has the potential to improve the accuracy of tumor diagnosis and treatment, and is of great significance for exploring personalized tumor therapy.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 138031"},"PeriodicalIF":8.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microstructure-controlled preparation of monodisperse In2O3 for excellent HCHO sensing with high selectivity, good anti-humidity and low detection limit","authors":"Zhen Sun ,&nbsp;Xueli Yang ,&nbsp;Wenlu Liu ,&nbsp;Yalin Zhang ,&nbsp;Zheng Hu ,&nbsp;Guofeng Pan ,&nbsp;Yehong Cheng","doi":"10.1016/j.snb.2025.138026","DOIUrl":"10.1016/j.snb.2025.138026","url":null,"abstract":"<div><div>Morphology control significantly improves the microstructure, increases the number of defects, tailors porosity, and optimizes the band gap of gas-sensing materials, profoundly affecting the adsorption and desorption capabilities for gas molecules. Herein, we prepared In(OH)₃ precursors via a hydrothermal method and rationally controlled the microstructure of In₂O₃ by varying annealing temperatures (400°C, 500°C, 600°C). Characterizations revealed that the porous cubic In₂O₃-500 (500°C) exhibited superior properties, including more balanced grain size and crystallinity, superior pore structure, more oxygen vacancies and adsorbed oxygen, and a narrower band gap compared to the surface-dense cubic In₂O₃-400 (400°C) and the porous spherical In₂O₃-600 (600°C). Gas-sensing tests demonstrated that three sensors displayed exceptional selectivity towards formaldehyde (HCHO), with rapid response times (1 s) to 100 ppm HCHO. Notably, In₂O₃-500, operating at a lower temperature (175°C), achieved the highest response (R_a/R_g = 200), good humidity resistance, and the lowest detection limit (30 ppb). The gas-sensing performance at 60 RH% showed that In₂O₃-500 still exhibited the highest response (161) to 100 ppm HCHO at 175℃, a decrease of less than 20 %. This study offers insights into the cost-effective and facile fabrication of high-performance HCHO gas sensors based on In₂O₃ materials.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 138026"},"PeriodicalIF":8.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144113438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and optimization of ordered porous interference enhanced substrate based on ordered porous layer interference system","authors":"Yanhua Tang,&nbsp;Na Ni,&nbsp;Nian Ding,&nbsp;Xinyu Yang,&nbsp;Wei Wei,&nbsp;Zeying Dou,&nbsp;Lei Jiang,&nbsp;Qianqian Su","doi":"10.1016/j.snb.2025.138018","DOIUrl":"10.1016/j.snb.2025.138018","url":null,"abstract":"<div><div>To overcome the problems of small pore size and limited reaction space of silica colloidal crystal (SCC) films, an ordered porous polystyrene (PS) -TiO₂ interference enhanced substrate with large pore size, high porosity, good interference efficiency and transparency was developed by introducing a TiO₂ interference enhanced layer with dip-coating method. The obtained substrate consists of three parts: the nanoporous structure layer, the PS substrate layer, and the TiO₂ layer between the two layers. The optical and mechanical properties of the obtained substrate was studied and optimized. The results showed that the optimal thickness of the PS substrate layer was 0.2 mm and that of TiO₂ interference enhanced layer was between 200 ∼ 400 nm. Combined with the ordered porous layer interference (OPLI) system, a continuous flow biosensor platform for real-time monitoring of the specific binding of <em>Staphylococcus aureus</em> protein A (SPA) and human serum immunoglobulin G (hIgG) was constructed to simulate an antibody screening test. The signal response of the system was about 3.8 times that of the system constructed with SCC substrate under the same conditions. The development of this new substrate provides the necessary support for the application expansion of the OPLI system, and had good application prospects in antibody screening, target protein binding performance evaluation and antibody drug development.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 138018"},"PeriodicalIF":8.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reversible pyrene-based fluorescent silica-polymeric hybrid materials: Utilization from selective probing of dopamine in cancer cells and zebrafish to cancer theranostic agents","authors":"Sobhan Chatterjee,&nbsp;Xiaojia Wu,&nbsp;Yuxin Bao,&nbsp;Rong Zhang,&nbsp;Yafei Zhang,&nbsp;Hong-Shang Peng","doi":"10.1016/j.snb.2025.138023","DOIUrl":"10.1016/j.snb.2025.138023","url":null,"abstract":"<div><div>The design of single organic-inorganic fluorescent materials for the selective detection of dopamine and its use as cancer theranostic agents opens up new possibilities. Dopamine detection has been widely studied in the diagnosis of neurodegenerative diseases and certain cancers, while dopamine-based photothermal (PT) and chemodynamic (CD) agents have been developed for cancer treatment. Considering the role of dopamine in humans, integrating the functions of sensing, capturing, and therapeutic use is clinically important. In this study, a fluorescent silica-polymeric hybrid nanomaterial (PS-PBA/PY) was first prepared by combining pyrene-1-carbaldehyde, 4-formylphenylboronic acid, and silica-melamine-resorcinol-formaldehyde materials. PS-PBA/PY can detect 1.2 µM dopamine in PBS buffer and exhibits high selectivity and reversibility in DMEM, FBS, HeLa cells, and zebrafish. Taking advantage of the dopamine and Cu^2 + binding capacity, PS-PBA/PY is effectively converted into PT and CD agents and conjugated with mitochondria-targeted ligands for tumor cell therapy. As a result, the therapeutic agents exhibit ∼69 % PT efficiency and toxic •OH production under NIR irradiation, which can effectively kill tumor cells through CDT/PTT combination therapy. This study not only provides a powerful nanoplatform for in <em>vitro</em> and in <em>vivo</em> tumor therapy, but also opens up new directions for the use of endogenous biomarker dopamine.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 138023"},"PeriodicalIF":8.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144113388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous photoacoustic monitoring of exhaled and airborne sevoflurane using adjustable branched cell and mirror chopper","authors":"Xueshi Zhang ,&nbsp;Yufeng Yang ,&nbsp;Yi Zeng ,&nbsp;Zhifu Yang ,&nbsp;Xiaoming Zhao ,&nbsp;Huiting Huan ,&nbsp;Xuesen Xu ,&nbsp;Lixian Liu ,&nbsp;Andreas Mandelis ,&nbsp;Huailiang Xu","doi":"10.1016/j.snb.2025.138027","DOIUrl":"10.1016/j.snb.2025.138027","url":null,"abstract":"<div><div>Sevoflurane is a popular volatile anesthetic agent. A high sensitivity and wide dynamic range T-type Branch-Enhanced Photoacoustic Spectroscopy (T-BEPAS) sensor was developed to monitor both exhaled sevoflurane (ppm to several percent) and airborne sevoflurane (ppb to ppm) for the therapeutic diagnosis of anesthetized patients and occupational exposure monitoring. To meet the requirements of small volume and low resonance frequency for sevoflurane monitoring, a coin-sized T-type Branched Photoacoustic Cell (T-BPAC) with the resonance frequency of only 1149 Hz was designed, whose cell response can be easily optimized by changing branches. With the incorporation of the branches, the sound wave propagation path was modified and resulting in an enhancement of thermal-acoustic coupling efficiency and microphone response. To maximize light utilization efficiency and avoid beam splitting, a mirror chopper was introduced to enable a single laser source to simultaneously monitor airborne and exhaled sevoflurane. For airborne sevoflurane monitoring, the T-BEPAS sensor achieved the detection limit of 2.1 ppb with a linear dynamic range of 2.1 ppb – 8000 ppm, corresponding to normalized noise equivalent absorption of 2.9 × 10⁻⁹ cm⁻¹WHz^−1/2. A theoretical analysis for optimizing the resonance frequency of the T-BPAC for exhaled sevoflurane detection was established, and the derived calibration method overcame the obstacle of cell response degradation with high-concentration sevoflurane. With the calibration method, the T-BEPAS sensor achieved the extended dynamic range of 4.8 ppb – 5 %. On-site clinical testing in the operating room showed the T-BEPAS sensor featured superior sensitivity and dynamic range compared to commercially available instruments.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 138027"},"PeriodicalIF":8.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distance-based paper analytical device for residual carbaryl pesticide quantification in food beverage samples","authors":"Kawin Khachornsakkul ,&nbsp;Tapparath Leelasattarathkul","doi":"10.1016/j.snb.2025.137984","DOIUrl":"10.1016/j.snb.2025.137984","url":null,"abstract":"<div><div>This article presents the first development of the distance-based paper analytical device (dPAD) for the quantitative detection of carbaryl (CBR) levels in food beverages samples. The detection method relies on the formation of an azo coupling compound between CBR and a synthetic diazonium compound in the presence of tetrabutylammonium hydroxide (TBAOH), resulting in the color change from colorless to orange. In our sensor, sample solution containing CBR is hydrolyzed by TBAOH deposited onto the sample zone of the device. Subsequently, the formed 1-napthol immediately flows along the detection zone where its reaction with pre-coated diazonium compound occurs. The change in distance length of the orange color is directly proportional to CBR levels. The dPAD sensor demonstrated a linear range between 70.0 and 110.0 ng mL⁻¹ (R² = 0.9983) with a detection limit (LOD) of 20.0 ng mL⁻¹, covering its maximum acceptable concentrations (MAC). Furthermore, the device offers a selective detection of CBR with no interferent effects. Additionally, our method shows remarkable accuracy and precision of the detection of CBR in food samples with a recovery study between 97.5 % and 103.3 % and the highest RSD of 2.55 %. Correspondingly, the results of the developed method are compatible with HPLC method at a 95 % confidence level. Therefore, this sensor demonstrates the high potential as a simple and affordable analytical device for the detection of CBR levels in various sample matrices without the need for peripheral equipment and skilled individuals, which shows a significant advancement in on-site analysis compared to the current analysis scenarios.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 137984"},"PeriodicalIF":8.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144113441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplex isothermal amplification-based immunochromatographic biosensor enhancing genuine-signal and suppressing pseudo-signal for on-site forensic test","authors":"Huyun Zhou ,&nbsp;Wenjing Hu ,&nbsp;Xiaomeng Zhang ,&nbsp;Xiaonan Liu ,&nbsp;Jiangwei Yan","doi":"10.1016/j.snb.2025.138025","DOIUrl":"10.1016/j.snb.2025.138025","url":null,"abstract":"<div><div>On-site forensic test can provide vital individual information to advance case progress. Nevertheless, specimens left in a crime scene are always limited, requiring identifying more targets from trace specimens to gain information maximally. Although multiplex loop-mediated isothermal amplification (LAMP) combined with immunochromatographic strip (ICS) possesses convenience and high sensitivity for on-site trace specimen analysis, it shares amplification substrates, reducing detectable amplicons from each target and weakening signal intensity on ICS. Besides, this combination fails to prevent false positive result from primer dimer. In this study, a multiplex immunochromatographic biosensor is established to enhance genuine-signal and suppress pseudo-signal for on-site forensic test. By using bio-labeled nucleotide for amplicon labeling, amplicon can be captured by nanoparticle probe and generate colorimetric signal on the ICS. Due to the incorporation of abundant bio-labeled nucleotides, bio-labels anchored on amplicon provide lots of recognition sites for nanoparticle probe capture, enhancing genuine-signal. As nucleotide cannot combine with oligonucleotide, although primer dimer still exists, it lacks corresponding bio-label to couple with nanoparticle probe, thereby suppressing pseudo-signal. Meanwhile, nucleotide is universal for amplification, avoiding bio-labeled primer resynthesis for each target and improving the flexibility. Moreover, the uracil DNA glycosylase-based anti-contamination strategy is integrated to eliminate carryover contamination. As a demonstration, species and sex of bloodstains’ donor can be identified simultaneously within 40 min. To facilitate on-site detection, LAMP mixture is lyophilized to avoid cold-chain transport. Accurate results are obtained using either liquid or lyophilized reagents, providing a promising tool for on-site nucleic acid analysis in forensic practice.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"441 ","pages":"Article 138025"},"PeriodicalIF":8.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144113439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}