Signal-amplified electrochemiluminescent aptasensor for ultrasensitive detection of Escherichia coli O157:H7 using ferrocene-quenched HOF-101 and hybridization chain reaction

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-10-05 DOI:10.1016/j.snb.2025.138916

Xinyan Zhang , Meifang Hua , Min Chen , Anhui Lin , Tingxiu Ye , Xiaomei Chen

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Abstract

Quick and accurate identification of Escherichia coli O157:H7 is essential for early foodborne illness detection and mitigation. An aptamer-based electrochemiluminescence (ECL) sensor was engineered in this study for fast and precise E. coli O157:H7 detection. Ferrocene (Fc) was identified as an effective quencher of the ECL signal from hydrogen-bonded organic framework material-101 (HOF-101) via electron transfer. Based on this mechanism, HOF-101 was employed as the ECL emitter and Fc as the quencher in the aptasensor design. Furthermore, signal amplification was significantly enhanced through HCR integration with the sensor, which permitted highly sensitive quantification of E. coli O157:H7. For E. coli O157:H7 quantification, the aptasensor showed linearity across 10–10⁶ CFU/mL (R²=0.993), achieving a remarkable detection limit (LOD) of 0.32 CFU/mL (S/N = 3). Additionally, the ECL aptasensor demonstrated high specificity and sensitivity toward E. coli O157:H7 and was successfully employed for pathogen quantification in spiked milk samples. This work not only establishes a novel approach for detecting foodborne pathogens but also highlights the potential of ECL-based strategies in food safety and environmental monitoring applications.

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二茂铁淬灭HOF-101和杂交链式反应用于超灵敏检测大肠杆菌O157:H7的信号放大电化学发光适体传感器

快速和准确地鉴定大肠杆菌O157:H7对于早期发现和减轻食源性疾病至关重要。本研究设计了一种基于适配体的电化学发光（ECL）传感器，用于快速、精确地检测大肠杆菌O157:H7。二茂铁（Fc）通过电子转移对氢键有机骨架材料-101 （HOF-101）的ECL信号进行了有效的猝灭。基于这一原理，采用HOF-101作为ECL发射极，Fc作为淬灭器进行了电感传感器的设计。此外，通过与传感器的HCR集成，信号放大显着增强，从而实现了大肠杆菌O157:H7的高灵敏度定量。对于大肠杆菌O157:H7，该传感器在10 ~ 106 CFU/mL范围内呈良好的线性关系（R2=0.993），检出限（LOD）为0.32 CFU/mL （S/N = 3）。此外，ECL配体传感器对大肠杆菌O157:H7具有较高的特异性和敏感性，并成功用于加标牛奶样品的病原体定量。这项工作不仅建立了一种检测食源性病原体的新方法，而且强调了基于ecl的策略在食品安全和环境监测应用中的潜力。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.