Sensors and Actuators B: Chemical最新文献_第7页

Assessing ferroptosis in Parkinson’s disease model by monitoring lipid peroxyl radical with an ER-targeted fluorescent probe er靶向荧光探针监测脂质过氧自由基对帕金森病模型铁下垂的影响

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-07-14 DOI: 10.1016/j.snb.2025.138331

Xinming Li, Junmin Xi, Yunlong Liu, Yu Liu, Qile Liu, Bo Li, Zejun Tian, Haidong Yan, Yaru Wang, Jianguo Fang

{"title":"Assessing ferroptosis in Parkinson’s disease model by monitoring lipid peroxyl radical with an ER-targeted fluorescent probe","authors":"Xinming Li, Junmin Xi, Yunlong Liu, Yu Liu, Qile Liu, Bo Li, Zejun Tian, Haidong Yan, Yaru Wang, Jianguo Fang","doi":"10.1016/j.snb.2025.138331","DOIUrl":"10.1016/j.snb.2025.138331","url":null,"abstract":"<div><div>Ferroptosis, driven by uncontrolled membrane lipid peroxidation, is closely related to the pathology of Parkinson’s disease (PD) and is considered a promising therapeutic target for PD. Lipid peroxyl radical (LOO•) is indispensable for the continuation and amplification of the lipid peroxidation process. Thus, fluorescent probes that can real-time monitor LOO• production in neural cells are highly desirable for understanding the role of ferroptosis in PD. Given that membrane lipid peroxidation is initially accumulated in endoplasmic reticulum (ER) during ferroptosis, we report herein the fabrication of the first ER-targeted fluorescent probes <strong>ER-Nap-LP1</strong> and <strong>ER-Nap-LP2</strong>, for selective detection of LOO•. In vitro experiments demonstrate that both probes exhibit high selectivity and good fluorescence turn-on response to multiple forms of LOO•, of which <strong>ER-Nap-LP2</strong> shows faster response and longer excitation/emission wavelength toward LOO•. <strong>ER-Nap-LP2</strong> has been effectively employed to track both exogenous and endogenous LOO• in PC12 cells and zebrafish. Furthermore, with the aid of <strong>ER-Nap-LP2</strong>, we found that ER LOO• is dramatically upregulated in rotenone-induced PD model. Pretreating the cells with ferroptosis inhibitors could eliminate excessively generated LOO•, further rescuing PC12 cells from rotenone-induced cell death, indicating the therapeutic value of ferroptosis inhibitors in PD. Overall, these results demonstrate that <strong>ER-Nap-LP2</strong> is a promising imaging tool to evaluate ferroptosis, which will facilitate the development of novel therapeutic strategies for PD.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"444 ","pages":"Article 138331"},"PeriodicalIF":8.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A high-performance and rapid-response ethanol gas sensor based on CoTiO3 nanoparticles decorated with phosphotungstic acid electron acceptor 基于磷钨酸电子受体修饰的CoTiO3纳米颗粒的高性能快速响应乙醇气体传感器

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-07-14 DOI: 10.1016/j.snb.2025.138333

Jinyong Gao, Shengnan Yin, Ying Yang, Feng Li, Dan Li, Hui Yu, Xiangting Dong, Wenyuan Pei, Tianqi Wang

{"title":"A high-performance and rapid-response ethanol gas sensor based on CoTiO3 nanoparticles decorated with phosphotungstic acid electron acceptor","authors":"Jinyong Gao, Shengnan Yin, Ying Yang, Feng Li, Dan Li, Hui Yu, Xiangting Dong, Wenyuan Pei, Tianqi Wang","doi":"10.1016/j.snb.2025.138333","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138333","url":null,"abstract":"In this paper, CoTiO<sub>3</sub> nanoparticles were prepared by hydrothermal and calcination method, and phosphotungstic acid (PW<sub>12</sub>) was introduced into CoTiO<sub>3</sub> nanoparticles. The introduced PW<sub>12</sub> can work as electron acceptor promote electrons and holes separation. The experimental results show that doping with appropriate amount of PW<sub>12</sub> can improve the gas sensitive property to ethanol of the CoTiO<sub>3</sub>. The response of CoTiO<sub>3</sub>/1%PW<sub>12</sub> to 100 ppm ethanol gas at the optimal operating temperature is 40.4, which is twice higher than that of pure CoTiO<sub>3</sub>, and the response and recovery time is also very rapid (1 s and 8 s). At the same time, other sensing properties such as repeatability and long term stability of the CoTiO<sub>3</sub>/PW<sub>12</sub> composite gas sensing materials were studied. In addition, the sensing mechanism was analyzed and discussed. The experiment proves that PW<sub>12</sub> can promote electron-hole separation and restrain their recombination improve gas sensitivity. This work provides a new idea for developing high-performance CoTiO<sub>3</sub> based gas sensor.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"8 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144640525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A GA-BP compensated PEDOT:PSS/MXene wearable NH3 detection device for food safety, environmental monitoring, and disease diagnosis 一种GA-BP补偿PEDOT:PSS/MXene可穿戴式NH3检测装置，用于食品安全、环境监测和疾病诊断

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-07-14 DOI: 10.1016/j.snb.2025.138326

Xinru Zhang , Yijie Wang , Lei Zhang , Xiaohai Zhang , Bao Hao , Xiaorui Liang , Helei Dong , Yongqiang Qin , Qiulin Tan

{"title":"A GA-BP compensated PEDOT:PSS/MXene wearable NH3 detection device for food safety, environmental monitoring, and disease diagnosis","authors":"Xinru Zhang , Yijie Wang , Lei Zhang , Xiaohai Zhang , Bao Hao , Xiaorui Liang , Helei Dong , Yongqiang Qin , Qiulin Tan","doi":"10.1016/j.snb.2025.138326","DOIUrl":"10.1016/j.snb.2025.138326","url":null,"abstract":"<div><div>The real-time and accurate detection of ammonia is critical for food freshness testing, agricultural monitoring, and respiratory health diagnostics. Recently, flexible gas sensors have garnered attention owing to their potential for integration into smart wearable electronics and display devices. However, the development of high-performance, low-power, and stable devices remains challenging. In this study, a chemoresistive sensor based on poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS)/MXene composites is designed, wherein the application of a genetic algorithm-optimized back propagation neural network to compensate for humidity leads to a significantly enhanced detection accuracy. Notably, the use of a genetic algorithm-optimized back propagation neural network reduces the detection error from 8.06 % to 1.3 % in the presence of 50 ppm NH<sub>3</sub>. A wireless Bluetooth monitoring system is integrated to enable real-time data transmission and remote monitoring. This sensor detects ammonia within the range of 0.6–1000 ppm, demonstrating an excellent stability, selectivity, and flexibility, thereby confirming the synergistic effects between PEDOT:PSS and MXene. Importantly, in application tests, the developed system reliably assesses meat spoilage and farm ventilation levels, indicating its high applicability and reliability. Successful human breath tests also open new possibilities for the application of this sensor in respiratory health monitoring. Ultimately, this study offers a convenient and efficient approach for food quality assurance, disease diagnosis, and environmental monitoring.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"444 ","pages":"Article 138326"},"PeriodicalIF":8.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144640526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flake-like WO3 decorated by PdRh nanoalloys: Sensing performance, DFT calculation, and machine learning-enhanced selectivity for triethylamine gas detection PdRh纳米合金修饰的片状WO3：传感性能、DFT计算和机器学习增强的三乙胺气体检测选择性

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-07-14 DOI: 10.1016/j.snb.2025.138329

Shixin Ma, Zhihua Zhao, Huiqin Li, Chen Chen, Lan Wu

{"title":"Flake-like WO3 decorated by PdRh nanoalloys: Sensing performance, DFT calculation, and machine learning-enhanced selectivity for triethylamine gas detection","authors":"Shixin Ma, Zhihua Zhao, Huiqin Li, Chen Chen, Lan Wu","doi":"10.1016/j.snb.2025.138329","DOIUrl":"10.1016/j.snb.2025.138329","url":null,"abstract":"<div><div>Bimetallic noble metal alloys attract extensive attention due to their excellent sensitization effects. Based on this, we synthesize monodisperse palladium (Pd) rhodium (Rh) alloy nanoparticles (NPs) and WO₃ spheres composed of flakes via a one-step hydrothermal method, followed by fabricating PdRh@WO₃ sensing materials with different mass percentages using an impregnation method. Testing revealed that at 240°C, the sensor based on 0.6 wt% PdRh@WO₃ exhibits a high response value of 240 toward 100 ppm triethylamine (TEA), approximately 33 times higher than that for ammonia and 62 times higher than formaldehyde, with response/recovery times of 20 s (s) and 27 s, respectively. The combination of density functional theory (DFT), gas chromatography-mass spectrometry (GC-MS) and diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) further confirms that the PdRh alloy promotes TEA adsorption and charge transfer. Two machine learning models, random forest (RF) and support vector machine (SVM), achieve accurate discrimination between TEA and interfering gases, further verifying the sensor's high selectivity. Moreover, the sensor demonstrates excellent applicability through real-time environmental simulations using an STM-32 microcontroller platform. This study provides a new solution for rapid detection and real-time monitoring of TEA in industrial production.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"444 ","pages":"Article 138329"},"PeriodicalIF":8.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144640527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoelectrochemical sensing Hela cell mitochondria released superoxide anion using a novel heterojunction formed by dispersing a wheel-shaped polyoxometalate on α-Fe2O3 nanoarray 利用在α-Fe2O3纳米阵列上分散轮状多金属氧酸盐形成的异质结，光电传感Hela细胞线粒体释放超氧阴离子

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-07-13 DOI: 10.1016/j.snb.2025.138324

Xinyao Zhu, Bingjie Li, Xiaoqiang Liu

{"title":"Photoelectrochemical sensing Hela cell mitochondria released superoxide anion using a novel heterojunction formed by dispersing a wheel-shaped polyoxometalate on α-Fe2O3 nanoarray","authors":"Xinyao Zhu, Bingjie Li, Xiaoqiang Liu","doi":"10.1016/j.snb.2025.138324","DOIUrl":"10.1016/j.snb.2025.138324","url":null,"abstract":"<div><div>An ultra-sensitive photoelectrochemical (PEC) sensor was developed by immobilizing negatively charged wheel-shaped polyoxometalate (Co<sub>16</sub>Mo<sub>16</sub>P<sub>24</sub>) clusters onto positively charged α-Fe<sub>2</sub>O<sub>3</sub> nanoarray modified F-doped Tin Oxide glass (α-Fe<sub>2</sub>O<sub>3</sub>NRs|FTO) through electrostatic adsorption. α-Fe<sub>2</sub>O<sub>3</sub>NRs|FTO has <em>a</em> large specific surface area to well disperse {Co<sub>16</sub>Mo<sub>16</sub>P<sub>24</sub>} clusters with excellent charge transfer behavior and PEC properties. {Co<sub>16</sub>Mo<sub>16</sub>P<sub>24</sub>} and α-Fe<sub>2</sub>O<sub>3</sub>NRs have been verified to be perfectly matched in energy levels, which form a high-efficiency heterojunction for promoting an effective separation of photogenerated electron-hole couples. Based on that, the PEC sensor exhibits a wide linear range from 0.005 nM to 5000 nM for hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) detection, with a sensitivity of 12.51 μA·nM<sup>−1</sup>, a detection limit of 0.093 nM, an excellent selectivity and long-term stability. Owing to the rapid conversion of superoxide radicals (O<sub>2</sub><sup>•⁻</sup>) to H<sub>2</sub>O<sub>2</sub>, the sensor can therefore indirectly measure O<sub>2</sub><sup>•⁻</sup> derived from the electron leakage in mitochondrial electron transfer chain (ETC) by detecting H<sub>2</sub>O<sub>2</sub> levels. To achieve a more reliable measurement, four inhibitors—rotenone, 2-monoacyltrifluoroacetone, antimycin A, and paclitaxel were utilized to induce the release of O<sub>2</sub><sup>•⁻</sup> from complexes I, II, III and IV in Hela cell mitochondria, respectively. By comparing the photocurrent changes caused by different inhibitors, we identify complex Ⅰ as the mostly likely site of electron leakage, which may provide meaningful information for the diagnosis of some oxidative stress diseases.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"444 ","pages":"Article 138324"},"PeriodicalIF":8.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EXPLORING PERFORMANCE LIMITS OF BINDING INHIBITION ASSAYS 探索结合抑制试验的性能限制

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-07-13 DOI: 10.1016/j.snb.2025.138321

Erika Hemmerová, Magdalena Čapková, Jiří Homola

引用次数: 0

Highly sensitive and selective colorimetric tin optode based on rhodamine B-cysteine immobilized on agarose membrane 琼脂糖膜固定化罗丹明b型半胱氨酸的高灵敏度、选择性比色锡光电器件

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-07-13 DOI: 10.1016/j.snb.2025.138323

Elaheh Mohammadi , Mohammad Javad Khodayar , Zahra Ramezani , Mehrnoosh Moosavi , Behzad Sharif Makhmalzadeh , Mehdi Safdarian

{"title":"Highly sensitive and selective colorimetric tin optode based on rhodamine B-cysteine immobilized on agarose membrane","authors":"Elaheh Mohammadi , Mohammad Javad Khodayar , Zahra Ramezani , Mehrnoosh Moosavi , Behzad Sharif Makhmalzadeh , Mehdi Safdarian","doi":"10.1016/j.snb.2025.138323","DOIUrl":"10.1016/j.snb.2025.138323","url":null,"abstract":"<div><div>An eco-friendly agarose-based optode was developed for the selective and sensitive detection of tin ions in aquatic and biological environments using UV-Vis spectroscopy. For this purpose, in a green synthesis method, cysteine was attached to rhodamine B and immobilized on an agarose membrane to prepare an optical sensor. For the first time, the agarose membranes were prepared using a specially designed homemade mold, which ensured uniform surface and dimensions, thereby significantly enhancing the reproducibility of the assays. The developed optode showed superior selectivity for tin ions over various interfering cations in an aqueous environment. The absorption studies indicated that the optode achieved its best performance at pH 4, enabling direct detection of Sn²⁺ ions within a concentration range of 0.5–25 × 10<sup>2</sup> ng mL<sup>−1</sup>. Colorimetric analysis revealed a detection limit of 4.96 ng mL⁻¹ for the optode, with intra-day and inter-day relative standard deviations (RSD %) of 1.01 % and 1.04 %, respectively. This optode successfully determined Sn²⁺ in environmental and biological samples without matrix separation.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"443 ","pages":"Article 138323"},"PeriodicalIF":8.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144612804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Confined enzyme probe-enhanced PER-Cas12a chemiluminescence biosensor for ultrasensitive detection of soluble PD-L1 in serum 限制性酶探针增强的PER-Cas12a化学发光生物传感器用于血清中可溶性PD-L1的超灵敏检测

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-07-13 DOI: 10.1016/j.snb.2025.138322

Lanlang Peng , Linshan Jiang , Ying Jiang , Tao Liu , Yan You , Shijia Ding , Jiangling Wu , Weixian Chen , Xiaojuan Ding

{"title":"Confined enzyme probe-enhanced PER-Cas12a chemiluminescence biosensor for ultrasensitive detection of soluble PD-L1 in serum","authors":"Lanlang Peng , Linshan Jiang , Ying Jiang , Tao Liu , Yan You , Shijia Ding , Jiangling Wu , Weixian Chen , Xiaojuan Ding","doi":"10.1016/j.snb.2025.138322","DOIUrl":"10.1016/j.snb.2025.138322","url":null,"abstract":"<div><div>Circulating soluble programmed death ligand 1 (sPD-L1), a critical immunomodulatory molecule, holds significant value for early tumor diagnosis, directing immunotherapy, and monitoring prognosis. In this study, we developed an ultra-sensitive and programmable confined enzyme probe-enhanced PER-Cas12a chemiluminescence (CEP-EPC) platform for detecting sPD-L1. This system initiates the trans-cleavage activity of Cas12a via aptamer-targeted recognition of sPD-L1 and a cascade primer exchange reaction (PER). Then, the activated Cas12a cyclically cleaves single-stranded DNA (ssDNA) on the confined enzyme probe, resulting in the release of HRP-labeled ssDNA. Following magnetic separation, the presence of HRP in the homogeneous solution markedly amplifies the chemiluminescence signal in the luminol-hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) system. The constructed sensor exhibits a linear range from 5 to 5 × 10<sup>3</sup> pg/mL, with a detection limit as low as 1.02 pg/mL. The analytical performance of the developed sensor was validated using 6 cell lines and 112 clinical test samples. Receiver operating characteristic (ROC) curve analysis demonstrated that the strategy could effectively distinguish tumor patients from healthy donors: non-small cell lung cancer (NSCLC, AUC = 0.8750), gastric cancer (AUC = 0.9325), pancreatic cancer (AUC = 0.9725), and breast cancer (AUC = 0.8500). Notably, a significant positive correlation (<em>r</em> = 0.6444, <em>p</em> < 0.0500) was observed between tissue PD-L1 expression and serum sPD-L1 levels in a cohort of 12 NSCLC patients, suggesting the potential application of this strategy in guiding PD-1/PD-L1 immune checkpoint blockade therapies. Collectively, these findings establish the CEP-EPC platform as a robust and promising tool for precision oncology.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"443 ","pages":"Article 138322"},"PeriodicalIF":8.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144612859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ratiometric SERS nanoprobe for ferroptosis monitoring in drug-induced liver injury via accurate detection of hydrogen peroxide in endoplasmic reticulum 比例SERS纳米探针通过准确检测内质网过氧化氢监测药物性肝损伤中的铁下垂

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-07-13 DOI: 10.1016/j.snb.2025.138306

Hailan Jia , Fengxian Wang , Chi Wang , Dong Wang , Chunyuan Zhang , Xuerui Fu , Keying Zhao , Jia Zhang , Lijia Liang , Yanting Shen

{"title":"Ratiometric SERS nanoprobe for ferroptosis monitoring in drug-induced liver injury via accurate detection of hydrogen peroxide in endoplasmic reticulum","authors":"Hailan Jia , Fengxian Wang , Chi Wang , Dong Wang , Chunyuan Zhang , Xuerui Fu , Keying Zhao , Jia Zhang , Lijia Liang , Yanting Shen","doi":"10.1016/j.snb.2025.138306","DOIUrl":"10.1016/j.snb.2025.138306","url":null,"abstract":"<div><div>Drug-induced liver injury (DILI) is a major liver dysfunction commonly caused by excessive or long-term administration of hepatotoxic drugs. The initiation and development of DILI have been closely related to ferroptosis. Revealing the internal relationship between ferroptosis and DILI is beneficial for the early diagnosis and therapy of DILI. Herein, a unique ratiometric surface-enhanced Raman scattering (SERS) nanoprobe was developed to explore the association between DILI and ferroptosis based on the variations of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) in endoplasmic reticulum (ER). The SERS nanoprobe is composed of an ER targeting peptide and an H<sub>2</sub>O<sub>2</sub> responsive Raman reporter (3-mercaptophenylboronic acid), which shows a remarkable ratiometric SERS change (I<sub>1071/998</sub>) for H<sub>2</sub>O<sub>2</sub> with high selectivity and sensitivity. Using the nanoprobe, the dynamic alterations of H<sub>2</sub>O<sub>2</sub> levels in ER within hepatocytes were successfully tracked throughout both the induction and inhibition of ferroptosis. Moreover, the complex interplay between ferroptosis and DILI in both cellular and murine models were elucidated. Complemented by Western blot analysis, the possible therapeutic effect of ferroptosis inhibition on DILI was confirmed by improving the Xc-/GSH/GPX4 antioxidant system. This study presents a novel approach for studying the relationship between ferroptosis and liver injury, thereby advancing DILI diagnosis and treatment.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"444 ","pages":"Article 138306"},"PeriodicalIF":8.0,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144613019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Peptide Multimer for Enhanced Imaging and Multivalent Detection of Hepatocellular Carcinoma 一种用于增强肝细胞癌成像和多价检测的新型多肽多聚体

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-07-13 DOI: 10.1016/j.snb.2025.138310

Shuo Feng, Xiaoli Wu, Eun-Young K. Choi, Tse-Shao Chang, Ruoliu Zhang, Sangeeta Jaiswal, Yuting Duan, Hui Jiang, Thomas D. Wang

{"title":"A Novel Peptide Multimer for Enhanced Imaging and Multivalent Detection of Hepatocellular Carcinoma","authors":"Shuo Feng, Xiaoli Wu, Eun-Young K. Choi, Tse-Shao Chang, Ruoliu Zhang, Sangeeta Jaiswal, Yuting Duan, Hui Jiang, Thomas D. Wang","doi":"10.1016/j.snb.2025.138310","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138310","url":null,"abstract":"The global incidence of hepatocellular carcinoma (HCC) is rising steadily, yet early detection methods remain a major clinical challenge due to genetic heterogeneity and variable target expression in these tumors. Conventional imaging approaches rely on non-specific or single targets, and often fail to achieve sufficient sensitivity or specificity, in particular, for early stage disease. A peptide multimer capable of simultaneously targeting 3 early HCC targets, including GPC3, CD44, and EpCAM, using a single biochemical construct was demonstrated. The multimer was labeled with either Cy5.5 for fluorescence imaging or Gd-DOTA for MRI to enable dual-modality detection. In vitro analysis using patient-derived HCC cell lines and organoids, and demonstrated significantly enhanced binding kinetics and affinity, including a 2.6-fold increase in fluorescence intensity and a 2.18-fold faster association rate compared with individual monomers. In vivo MRI in orthotopic patient-derived xenograft (PDX) models, both with and without cirrhosis, showed a peak tumor-to-background ratio of 3.05 at 0.5 hours post-injection and rapid renal clearance by about 4 hours. Ex vivo immunofluorescence of human liver specimens yielded 87% sensitivity and 80% specificity for distinguishing HCC from cirrhosis. These findings highlight multimer potential as a clinically translatable platform to improve early HCC diagnosis via enhanced molecular imaging.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"694 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144612803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0