Sensors and Actuators B: Chemical最新文献

{"title":"A Visual Isothermal Platform for the Rapid Detection of Clostridioides difficile in Fecal Using Locked Nucleic Acid-Modified Split DNAzyme Probes Facilitated by Recombinase Polymerase Amplification","authors":"Zhuo Chen, Yuexin Yao, Ruiqin Luo, Xianru Xia, Danni Wang, Chengyu Li, Xiaoming Sun","doi":"10.1016/j.snb.2025.138312","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138312","url":null,"abstract":"Early, rapid, and accurate diagnosis is of great significance for the timely treatment of <em>C. difficile</em>. This study constructed a visual isothermal analysis platform for <em>C. difficile</em> in feces based on recombinase polymerase amplification (RPA) and locked nucleic acid (LNA)-modified four sets of split DNAzyme probes, which can complete nucleic acid amplification and detection within 40<!-- --> <!-- -->minutes. RPA technology avoids the need for thermal cycling instruments, and greatly reduces amplification time. The combination of LNA-modified probes and target nucleic acid is extremely stable, and neither temperature nor enzyme influence can dissociate it. The split G-quadruplex/hemin DNAzyme maintains its catalytic capability to oxidize the colorless substrate 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS²⁻) into its green radical form ABTS∙⁻. Of particular significance, LNA-modified split DNAzyme probes with the help of several enzymes in the RPA system can accurate and stable capture of target double-stranded DNA, subsequently facilitating the DNAzyme formation. This innovative approach significantly streamlines the experimental procedure by minimizing operational complexity and reducing processing time. The assay outcomes are readily discernible through direct visual observation and can be conveniently documented using a smartphone. Therefore, this method of visual detection for <em>C. difficile</em> established in this study has the advantages of being time efficient, and having a high sensitivity and specificity.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"35 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144603750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser Diode-Induced in Situ Solid-State Synthesis of Graphitized Magnetic Nanostructures as Novel Nanozyme Catalysts","authors":"Achraf Berni, Juan José García-Guzmán, Rodrigo Alcántara, José María Palacios-Santander, Aziz Amine, Laura Cubillana-Aguilera","doi":"10.1016/j.snb.2025.138311","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138311","url":null,"abstract":"Laser-induced graphene (LIG) is a revolutionizing nanomaterial as a tunable, 3D-networked, highly conductive, which makes it a perfect platform for hosting functional nanostructures and enhancing catalytic activity. In this work, a one-step Near-UV laser-assisted graphitization of magnetic nanomaterials —Fe₃O₄-LIG, Ag-Fe₃O₄-LIG, and Cu-Fe₃O₄-LIG— has been developed that forms a universal four-in-one nanozyme catalytic platform with peroxidase (POD), oxidase (OXD), catalase (CAT), and laccase (LAC) activities. The laser-driven process enables simultaneous graphitization, metal oxidation, and nanostructuration, offering an inexpensive, green, and scalable option for multi-step synthesis protocols. The nanozymes thus obtained were well characterized by scanning/transmission electron microscopy (SEM/TEM) for morphology, X-ray diffraction (XRD) for crystallinity, and Raman spectroscopy for graphitic character. An exhaustive dual characterization via optical as well as electrochemical methods was employed to assess and monitor the nanozyme material's catalytic activities. Out of all synthesised materials, Ag-Fe₃O₄-LIG exhibited the highest oxidase and peroxidase mimicking activity, whereas Cu-Fe₃O₄-LIG exhibited enhanced catalase and laccase-like activity. To investigate the laccase activity further, Cu-Fe₃O₄-LIG was employed with phenolic compounds like dopamine, adrenaline, and L-dopa, which oxidized the substrates into aminochromes. As a proof of concept, taking advantage of the electroactivity of adrenochrome, adrenaline was sensed using a 3D porous LIG sensor. The sensor exhibited great sensitivity in the cathodic region with a linear range of 0.5 to 10<!-- --> <!-- -->μM and a detection limit of 0.23<!-- --> <!-- -->μM. This work creates a groundbreaking nanozyme platform with broad implications in biosensing, demonstrating the strength of laser-assisted soft synthesis for future generations of enzyme-mimicking materials.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"109 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coaxial electrospinning In2O3/TiO2 core-shell nanofibers for enhanced triethylamine sensing","authors":"Jun He, Xiaoyu Qi, Jingjing Liu, Hao Zhang, Zhongliao Wang, Shuwang Duo","doi":"10.1016/j.snb.2025.138313","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138313","url":null,"abstract":"Triethylamine (TEA), a hazardous and flammable compound, poses significant risks to both human health and the environment. Therefore, the development of precise and rapid methods for detecting TEA in complex environments is imperative. In this study, In₂O₃/TiO₂ core-shell nanofibers (NFs) with different mass ratio were successfully obtained by coaxial electrospinning. Through the characterization and performance comparison of different ratio for [Ti]: [In] (1:2, 1:1, 2:1 and 3:1), it was found that the TEA sensor based on Ti₂In₁ owned the best sensing performance. Specifically, it demonstrated the highest response value of 250.8 to 200 ppm TEA at 280℃, the shortest response/recovery time (14/21<!-- --> <!-- -->s) and the smallest stability fluctuation deviation (5.1%). Notably, the sensor exhibited excellent selectivity and anti-interference capability by distinguishing TEA from similar gases such as trimethylamine, dimethylamine, and ammonia. Compared with pristine TiO₂ and In₂O₃ nanofibers, the superior performance of the Ti₂In₁ sensor can be attributed to its unique core (In₂O₃)-shell (TiO₂) nanofiber architecture and the interfacial electron transfer processes occurring between these components, which were confirmed through ultraviolet photoelectron spectroscopy (UPS) and X-ray photoelectron spectroscopy (XPS) tests. Furthermore, electrochemical impedance spectra (EIS) and Mott-Schottky (MS) Nyquist measurement confirmed the sensor's excellent electron migration properties. These factors facilitated the adsorption, transport, and reaction of gas molecules, thereby in the enhancement of the TEA sensing performance. Moreover, density functional theory (DFT) calculation was executed and the same results as the experiment were obtained. This study paves the way for novel explorations into morphological manipulation and intrinsic mechanisms underlying TEA sensing materials.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"151 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-cost hydrogen sensor with the transient response of a quartz tuning fork","authors":"","doi":"10.1016/j.snb.2025.138317","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138317","url":null,"abstract":"Amidst the global transition toward clean energy, hydrogen (H2) has emerged as one of the most promising sustainable energy carriers due to its high e…","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"13 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-Assisted Point of Care Testing for Colorectal Cancer Screening: Application of Split Light-Up Aptamer for DNA Methylation Detection","authors":"Songcheng Yu, Tao Liu, Kaiyang Zhang, Yongjun Wu, Lihua Ding","doi":"10.1016/j.snb.2025.138303","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138303","url":null,"abstract":"Aberrant DNA methylation plays a crucial role in carcinogenesis and serves as a valuable biomarker for the early detection of colorectal cancer. However, most current DNA methylation detection methods still rely on fluorescent probes labeled with both a fluorophore and a quencher, which are costly and require time-consuming synthesis procedures. Therefore, there is an urgent need for convenient, visual, and rapid diagnostic platforms utilizing label-free probes for DNA methylation analysis. Herein, we present a low-cost and portable smartphone-based point-of-care testing (POCT) platform for rapid and visual detection of DNA methylation by utilizing split light-up DNA aptamers as label-free signal transducers in conjunction with the assistance smartphone camera. Compared to conventional fluorescent probes, the split light-up aptamers offer simplified synthesis and eliminate the need for labeling. Based on the RGB values of the images, a linear relationship was established between fluorescence intensity and the logarithm of DNA methylation concentration over the range of 10 to 5000<!-- --> <!-- -->nM, with a detection limit of 0.769<!-- --> <!-- -->nM. Furthermore, the platform performed well in real samples within 20<!-- --> <!-- -->min. We believe that the POCT platform has great potential for biomedical applications and public health surveillance in the face of emerging infectious diseases.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"147 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144594725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intraoperative pathological diagnosis for breast cancer with a polarity fluorescent probe targeting lipid droplets","authors":"Fangfang Meng, Qing Lyu, Wenjie Zhu, Junyi He, Peng Gao, Ling Gao, Xiaoqiang Yu","doi":"10.1016/j.snb.2025.138305","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138305","url":null,"abstract":"Precise intraoperative pathological diagnosis is crucial for effective surgical management. However, conventional method based on hematoxylin-eosin (H&amp;E) staining of frozen sections often suffers from accuracy limitations due to a variety of factors. In this study, we developed a novel and efficient method utilizing a lipid droplets (LDs)-targeted fluorescent probe, named <strong>NA-LD</strong>, for intraoperative pathological diagnosis of breast cancer. Owing to the intramolecular charge transfer (ICT) effect, <strong>NA-LD</strong> exhibited ultrasensitive fluorescence responses to environmental polarity changes, enabling high-fidelity imaging of LDs. Interestingly, the probe was capable of differentiating breast cancer cells from normal ones by visualizing the difference of LDs characteristics, including polarity, number and size. Furthermore, using <strong>NA-LD</strong> the discrimination of tumor in a mouse model was achieved. Most importantly, it has been successfully applied in determining the benign or malignant nature of the intraoperative breast lesion, as well as in discriminating ductal carcinoma in situ (DCIS) and invasive ductal carcinoma (IDC). Therefore, this work may provide novel insights for the precise diagnosis and personalized treatment of breast cancer patients.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"692 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144594724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced cataluminescence sensing of iso-butyraldehyde via phase-specific oxygen vacancies engineering in BaTiO3","authors":"Na Chen, Ziyi Yu, Yun Cai, Zhiquan Wu, Yulin Jiang, Qingsong Chen, Qianchun Zhang, Jingxin Wang, Runkun Zhang","doi":"10.1016/j.snb.2025.138308","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138308","url":null,"abstract":"Understanding the relationship between oxygen vacancies (V_OS) and gas sensing performance is pivotal for advancing high-performance gas sensors. In this study, we synthesized two cubic phase barium titanate (BaTiO₃) samples (BTO-1 and BTO-2) and one tetragonal phase BaTiO₃ sample (BTO-3). BTO-1 was subjected to additional nitrogen calcination to enhance its V_O concentration, while BTO-2 was prepared without this calcination step. Morphological and structural analyses confirmed that BTO-1 possesses the highest V_O concentration and surface-adsorbed oxygen content. Gas sensing investigations revealed that all BaTiO₃ samples exhibit excellent cataluminescence specificity toward <em>iso</em>-butyraldehyde (IBD), with response sensitivity that is strongly correlated to V_O concentration. Notably, BTO-1 demonstrated the highest sensitivity, achieving a detection limit of 7.3<!-- --> <!-- -->mg/m³. Density functional theory calculations showed that cubic BaTiO₃ more readily forms V_O compared to its tetragonal counterpart, and the introduction of V_O enhances the adsorption and activation of both oxygen and IBD molecules. Reaction pathway analyses indicated that the primary oxidative decomposition of IBD on BaTiO₃ surfaces produces formic acid and acetone, with triplet acetone likely serving as the luminescent intermediate. These findings emphasize the significance of crystal phase-dependent V_O engineering in enhancing sensor performance and establish BaTiO₃-based materials as promising candidates for precise IBD detection. This work provides critical insights into designing next-generation gas sensors with tailored V_O properties for environmental and industrial applications.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"22 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144594727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel intelligent sensing strategy: Integration of metal-doped carbon dots nanozymes and machine learning for rapid screening of biothiols in disease","authors":"Mingming Wei,&nbsp;Mei Yang,&nbsp;Han Leng,&nbsp;Yang Shu","doi":"10.1016/j.snb.2025.138304","DOIUrl":"10.1016/j.snb.2025.138304","url":null,"abstract":"<div><div>Biothiols play a crucial role in signal transduction and cellular metabolism, and their accurate detection is essential for biomarker monitoring and disease diagnosis. However, due to the similar chemical structures of different biothiols, traditional detection methods often suffer from false positive signals, mutual interference, expensive instrumentation, and complex operations. Array sensing technology provides an ideal solution as an efficient multi-channel parallel detection method that can analyze multiple targets simultaneously. A nanozyme-based colorimetric sensor array is proposed with the aim of providing a high-throughput, high-sensitivity, high-selectivity, low-cost, and convenient solution for the detection of biothiols. Metal ion-doped carbon dots (M-CDs) were employed as sensing units to mimic peroxidase activity, catalyzing the oxidation reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). Different biothiols exhibited varying degrees of inhibition on the catalytic activity of M-CDs, resulting in unique fingerprint patterns. The method was integrated with various machine learning algorithms, including linear discriminant analysis (LDA), hierarchical clustering analysis (HCA), artificial neural networks (ANN), K-nearest neighbors (KNN), decision trees (DT), and support vector machines (SVM), accurately distinguished eight biothiols with a detection limit of 40 nM. This approach not only overcomes the limitations of traditional techniques but also enables efficient detection in complex biological samples (such as serum, urine, cells, and bacteria), providing a sensitive and straightforward technological platform for biothiol monitoring in medical diagnostics.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"444 ","pages":"Article 138304"},"PeriodicalIF":8.0,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144594726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature driven p- to n-type transition of In2O3/NixOy nanocavity structure for H2 gas sensors","authors":"Yuxiao Jiang, Kelin Hu, Jing Zhang, Tao He, Bin Yu, Xinyuan Li, Jiarong Yang","doi":"10.1016/j.snb.2025.138307","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138307","url":null,"abstract":"This research focused on the synthesis of In₂O₃/Ni_xO_y nanocomposites utilizing the conventional template method. The resulting gas sensor, designed for H₂ detection, demonstrates a sensing behavior that is dependent on temperature. Various characterization techniques were employed to analyze the synthesized In₂O₃/NiO composite materials. The results obtained from tests conducted at different temperatures reveal that the sensor maintains a stable n-type response for H₂ at temperatures exceeding 240°C, while exhibiting an atypical p-type response at lower temperatures. This transition in adsorption behavior enables the In₂O₃/Ni_xO_y composites to function as a dual-mode sensor capable of detecting H₂. The temperature-induced transformation is likely attributable to the interplay of several mechanisms. This article elucidates this phenomenon by examining factors such as activation energy, TGA/DSC, adsorbed oxygen, heterojunction formation, and humidity effects. This study contributes to the understanding of the transformation mechanisms underlying MOS gas sensing behavior.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"1 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144594737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ nanoscale thickness monitoring of passive films based on electrochemical-surface plasmon resonance sensing technology","authors":"Ruizhi Fan, Ming Lin, Rongji Zheng, Wanbin Chen, Wei Peng, Zewei Luo, Yunze Xu, Mengdi Lu","doi":"10.1016/j.snb.2025.138309","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138309","url":null,"abstract":"Gold (Au) is frequently employed as a critical connector component in precision electronic instruments due to its exceptional stability. However, it remains susceptible to corrosion deterioration in complex environments such as in harsh marine settings. The passive film that forms on the Au surface is vital for corrosion protection, making the monitoring of the structure and thickness of this nanoscale film essential for ensuring the safe operation of the Au. A terminal-reflective optical-fiber structure was utilized both as the working electrode for an electrochemical (EC) sensing and as the surface plasmon resonance (SPR) sensing element, thus creating an integrated dual-function optical fiber EC-SPR sensor. This sensor enabled detection of the formation of Au passive films at various constant voltages in a high-salinity environment, alongside monitoring changes in nanoscale thickness. Based on the polarization curves, the passivation of Au was conducted at different constant voltages. During this process, the EC signal and the SPR resonance wavelength were monitored in real time to track the passivation progression. The in-situ EC-SPR spectra revealed the dynamic changes occurring on the Au surface during the formation. Notably, as the voltage increased, the thickness of the passive film correspondingly increased, yielding equivalent passive film thicknesses of 10 (±0.3) nm, 14 (±0.4) nm, and 18 (±1.1) nm at constant voltages of 0.4<!-- --> <!-- -->V, 0.6<!-- --> <!-- -->V, and 0.8<!-- --> <!-- -->V, respectively. This integrated optical fiber dual-function sensor facilitates online measurement of nanoscale Au passive film thickness, providing a novel monitoring approach for corrosion detection and the safe operation of deep-sea equipment.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"13 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144603751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}