Sensors and Actuators B: Chemical最新文献_第6页

Host-guest recognition-regulated TiO2@In2S3 S-scheme heterojunction photoactivity: A PEC-EC dual-mode split-type aptasensor for malathion

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-06 DOI: 10.1016/j.snb.2025.137574

Ben-Fang Xu , Jiang-Zhou Wu , Pei Song , Qin-Ying Ai , Ai-Jun Wang , Li-Ping Mei , Yadong Xue , Jiu-Ju Feng

{"title":"Host-guest recognition-regulated TiO2@In2S3 S-scheme heterojunction photoactivity: A PEC-EC dual-mode split-type aptasensor for malathion","authors":"Ben-Fang Xu , Jiang-Zhou Wu , Pei Song , Qin-Ying Ai , Ai-Jun Wang , Li-Ping Mei , Yadong Xue , Jiu-Ju Feng","doi":"10.1016/j.snb.2025.137574","DOIUrl":"10.1016/j.snb.2025.137574","url":null,"abstract":"<div><div>Ensuring the sensitive and rapid detection of residual malathion (MLT) levels in both environment and food is crucial for ecological environment and food safety. Herein, host-guest recognition-regulated the photoactivity of TiO<sub>2</sub>@In<sub>2</sub>S<sub>3</sub> S-scheme heterojunction was applied to construct a photoelectrochemical (PEC) and electrochemical (EC) dual-mode split-type aptasensor for malathion bioassay. The S-scheme charge transfer mechanism, in conjunction with sulfur vacancies in the TiO<sub>2</sub>@In<sub>2</sub>S<sub>3</sub> heterojunction, not only broadens the light absorption range, but also facilitates efficient separation and migration of photogenerated carriers, thus enhancing the original photocurrent response of the sensor. In the presence of MLT, rhodamine B-labeled MLT aptamer (RhB-apta)-MLT conjugates were formed and acted as the guest, which was recognized by β-cyclodextrin as the host on the electrode surface. Leveraging the excellent electroactivity of RhB and its sensitization of TiO<sub>2</sub>@In<sub>2</sub>S<sub>3</sub>, a PEC-EC dual-mode aptasensor for MLT assay was achieved. The proposed aptasensor exhibits a wide linear range (0.001 ∼ 50 ng mL<sup>−1</sup>), with a detection limit of 0.12 pg mL<sup>−1</sup> in PEC mode and 0.62 pg mL<sup>−1</sup> in EC mode. The combination of the two detection signals can complement and validate each other, thereby improving the sensitivity, accuracy, and reliability of malathion detection.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"434 ","pages":"Article 137574"},"PeriodicalIF":8.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gas concentration prediction based on temporal attention mechanism in temporal convolutional networks

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-06 DOI: 10.1016/j.snb.2025.137562

Pengfei Jia , Zhicong Chen , Guosheng Mao , Yiyi Zhang , Jiefeng Liu , Min Xu

{"title":"Gas concentration prediction based on temporal attention mechanism in temporal convolutional networks","authors":"Pengfei Jia , Zhicong Chen , Guosheng Mao , Yiyi Zhang , Jiefeng Liu , Min Xu","doi":"10.1016/j.snb.2025.137562","DOIUrl":"10.1016/j.snb.2025.137562","url":null,"abstract":"<div><div>To predict indoor air quality, we can analyze the concentrations of gas present in the air. Accurate prediction of gas concentrations can help individuals identify the presence of harmful gas in the environment, thereby preventing potential accidents. Previous studies have utilized electronic nose (E-nose) in conjunction with traditional neural networks to obtain gas concentration information; However, these neural networks often experience a decline in prediction accuracy when handling longer time series data, failing to meet expected outcomes. To enhance prediction accuracy, this study introduces an innovative Temporal Convolutional Network (AGT-TCN), designed for the prediction of mixed gas concentrations in E-nose. AGT-TCN comprises a temporal convolutional network (TCN), gated recurrent units (GRU), a temporal attention mechanism and residual convolutions. In the experiments, we employed data from carbon monoxide and ethylene mixed gas (CO-ethylene) for concentration predictions and compared the results with baseline models including TCN, GRU, Long and short-term memory network (LSTM), convolutional neural network - long short-term memory network (CNN-LSTM) and Long Short-Term Memory-Transformer (LSTM-Transformer). The results demonstrate that AGT-TCN outperforms baseline in terms of prediction accuracy. This further confirms the applicability of the AGT-TCN in the early prediction of CO-ethylene concentrations.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"433 ","pages":"Article 137562"},"PeriodicalIF":8.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface photografted thermoresponsive hydrogel microvalves on PDMS/silicon hybrid membrane for light-actuated localized chemical release

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-06 DOI: 10.1016/j.snb.2025.137559

Nafis Mustakim, Mayank Pandey, Sang-Woo Seo

{"title":"Surface photografted thermoresponsive hydrogel microvalves on PDMS/silicon hybrid membrane for light-actuated localized chemical release","authors":"Nafis Mustakim, Mayank Pandey, Sang-Woo Seo","doi":"10.1016/j.snb.2025.137559","DOIUrl":"10.1016/j.snb.2025.137559","url":null,"abstract":"<div><div>On-demand localized chemical release holds great promise for various biomedical applications, including drug delivery systems and neurotransmitter-based prostheses. Temperature-sensitive poly(n-isopropylacrylamide) (PNIPAM)-based hydrogel has been widely implemented in on demand drug delivery devices. In particular, surface photografted PNIPAM has the merit of integrating hydrogel film onto a targeted surface through a photo-initiated crosslinking process. This paper explores the integration of surface photografted PNIPAM microvalves into a PDMS (Polydimethylsiloxane)/silicon hybrid membrane to create a device platform for light-actuated localized chemical release. We have developed unique fabrication processes to create a PDMS/silicon hybrid membrane and to photograft PNIPAM using benzophenone absorbed in PDMS film. The flow of chemicals in macroscopic pores can be actuated through the surface photografted microvalve. Our platform demonstrates NIR light-actuated localized chemical release by incorporating Polypyrrole (PPy) as a photothermal element. By adjusting several actuation parameters, including laser power and pulse duration, we have investigated the operation of the microvalve. The opening and closing times were measured within 2 s. Each actuation released approximately 2–10 % of the peak model chemical from the reservoir, depending on NIR laser power levels ranging from 20 to 60 mW/mm<sup>2</sup>. Furthermore, repeated activation of the microvalves exhibited a minimum variation (∼8 %) in the release profile. The ability to control chemical release at a micron-scale area using light will be of great interest for various neurotransmitter-based prosthetic applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"433 ","pages":"Article 137559"},"PeriodicalIF":8.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly sensitive colorimetric detection of ammonia and respiratory ammonia based on ammonia induced perylene diimide anion radical π-dimer dissociation

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-06 DOI: 10.1016/j.snb.2025.137555

Yahua Zhu , Gengwen Yin , Wei Li , Rui Tan , Wenlong Hou , Xueqiang Liu , Yuzhen Zhao , Haiquan Zhang

{"title":"Highly sensitive colorimetric detection of ammonia and respiratory ammonia based on ammonia induced perylene diimide anion radical π-dimer dissociation","authors":"Yahua Zhu , Gengwen Yin , Wei Li , Rui Tan , Wenlong Hou , Xueqiang Liu , Yuzhen Zhao , Haiquan Zhang","doi":"10.1016/j.snb.2025.137555","DOIUrl":"10.1016/j.snb.2025.137555","url":null,"abstract":"<div><div>Ammonia is not only a crucial respiratory biomarker for chronic kidney diseases, but also an important raw material in chemical industry. Ammonia has important economic value, however, it also has harmful effects. Therefore, the sensitive and rapid detection of low concentration ammonia is always a hot and challenging topic in sensing field. Recently, we discovered a type of perylene diimide anionic radical π-dimers which the absorbance spectrum depends on the hydrogen bond between two radical molecules. Herein, the colorimetric detection of NH<sub>3</sub>·H<sub>2</sub>O, NH<sub>3</sub> gas, and respiratory NH<sub>3</sub> is achieved through the color changes resulting from the hydrogen bond in the π-dimer being broken by NH<sub>3</sub>. The π-dimer solution achieving a lowest detection limits of 0.26 ppb for NH<sub>3</sub>·H<sub>2</sub>O, and provided high precision recognition with approximately 90 % accuracy based on UV–vis spectrum and color change. The colorimetric films based on the π-dimer/silica gel can detect NH<sub>3</sub> gases at ppb level, and achieving high precision recognition with about 94 % accuracy. The cotton fibers dyed by π-dimers were successfully used in detecting 830 ppb NH<sub>3</sub> gas (the NH<sub>3</sub> concentration in exhaled breath of early chronic kidney diseases) based on the visible color changes. We believe that these environmental sensitive radical derivatives can serve as candidate colorimetric probes, which offers a novel and non-invasive approach for the early warning of chronic kidney disease.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"433 ","pages":"Article 137555"},"PeriodicalIF":8.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143569849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and sensitive identification of Pseudomonas aeruginosa through a fluorescent probe utilizing a magnetic 3D DNA walker and APE1 enzyme-powered cascade signal amplification

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-06 DOI: 10.1016/j.snb.2025.137585

Kun Wang , Siyu Jiang , Yufeng Hu , Tianhan Kai

{"title":"Rapid and sensitive identification of Pseudomonas aeruginosa through a fluorescent probe utilizing a magnetic 3D DNA walker and APE1 enzyme-powered cascade signal amplification","authors":"Kun Wang , Siyu Jiang , Yufeng Hu , Tianhan Kai","doi":"10.1016/j.snb.2025.137585","DOIUrl":"10.1016/j.snb.2025.137585","url":null,"abstract":"<div><div>The efficient and accurate identification of Pseudomonas aeruginosa (PA) is vital for reducing the risk of disease spread, and nucleic acid detection (NAT) is an excellent approach for the early diagnosis of this infectious agent. Herein, we created a fluorescent probe utilizing a magnetic 3D DNA walker that incorporates APE1 enzyme-driven signal enhancement (MDWAE) to quickly and precisely identify PA 16S rRNA. The DNA walker, activated by PA 16S rRNA, generates cleavage products that hybridize with molecular beacons containing apurinic sites, enabling APE1-mediated fluorescence amplification. This cascade process achieved PA determination within one hour with a detection limit of 1 CFU/mL while effectively differentiating PA from other bacteria. Recovery tests further confirmed that the cascade fluorescence probe is capable of detecting PA in spiked milk and orange juice samples, pointing to its potential for significant application in food safety assessments, environmental monitoring, and disease diagnosis.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"433 ","pages":"Article 137585"},"PeriodicalIF":8.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A high signal-to-noise ratio NIR fluorescent probe for viscosity detection in NAFLD diagnosis

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-06 DOI: 10.1016/j.snb.2025.137581

Junlei Hao , Suntao Shi , Yaoling Zhang , Xiao Li , Xiaowen Ren , Jia Gao , Shuijuan Liu , Haijuan Zhang , Jiang Wu , Baoxin Zhang

{"title":"A high signal-to-noise ratio NIR fluorescent probe for viscosity detection in NAFLD diagnosis","authors":"Junlei Hao , Suntao Shi , Yaoling Zhang , Xiao Li , Xiaowen Ren , Jia Gao , Shuijuan Liu , Haijuan Zhang , Jiang Wu , Baoxin Zhang","doi":"10.1016/j.snb.2025.137581","DOIUrl":"10.1016/j.snb.2025.137581","url":null,"abstract":"<div><div>Viscosity is a critical parameter within the cellular environment, playing a fundamental role in various biochemical processes and the functional integrity of organelles. Abnormal viscosity fluctuations are often linked to conditions such as Non-alcoholic fatty liver disease (NAFLD), diabetes, and neurodegenerative disorders. Thus, monitoring viscosity variations in biological systems is vital for disease-related research. In this study, we have styled and synthesized <strong>VFP-4</strong>, a near-infrared (NIR) fluorescent probe with a high signal-to-noise ratio (436.4-fold), which demonstrates highly sensitive responsiveness to viscosity changes while remaining unaffected by pH and various interfering substances. Furthermore, <strong>VFP-4</strong> was successfully employed in the imaging of viscosity in live cells treated with dexamethasone (DXM) or lipopolysaccharide (LPS), showing precise targeting of lipid droplets (LDs). Notably, due to its NIR emission and exceptional sensitivity to viscosity, <strong>VFP-4</strong> can detect real-time viscosity changes in zebrafish and mouse models of NAFLD while exhibiting excellent photostability during imaging. This work holds significant potential for advancing our understanding of cellular processes and developing novel diagnostic and therapeutic strategies by enabling real-time, non-invasive monitoring of intracellular viscosity changes.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"433 ","pages":"Article 137581"},"PeriodicalIF":8.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated flexible dual-mode optical-electrochemical sensing microcatheter platform for inflammation monitoring

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-06 DOI: 10.1016/j.snb.2025.137514

Atul Sharma , Nafize Ishtiaque Hossain , Sameer Sonkusale

{"title":"An integrated flexible dual-mode optical-electrochemical sensing microcatheter platform for inflammation monitoring","authors":"Atul Sharma , Nafize Ishtiaque Hossain , Sameer Sonkusale","doi":"10.1016/j.snb.2025.137514","DOIUrl":"10.1016/j.snb.2025.137514","url":null,"abstract":"<div><div>Timely monitoring of inflammation following transplant surgery is essential to prevent complications such as ischemia or death. Centralized testing often leads to delays. Point-of-care immunoassays offer some promise. However, they lack the form factor and the performance needed for localized monitoring of inflammation. This work demonstrates a novel dual-mode microcatheter platform for <em>in situ</em> quantification of inflammatory biomarkers, such as interleukin- 6 (IL-6). By combining an electrochemical approach, namely differential pulse voltammetry (DPV), and an optical approach, namely localized surface plasmon resonance (LSPR), the platform enables reliable, on-demand, precise monitoring of inflammation. The novelty of the approach is using surgical sutures for electrochemical sensing and its co-integration with an optical fiber for LSPR sensing in a miniaturized and flexible microcatheter platform. This platform can locally probe the surgical or wound site for inflammation monitoring, providing high precision and reliability. The dual-mode approach enhances detection accuracy, precision, and dynamic range. The dual-mode microcatheter sensor exhibited a broad dynamic range from 0.1 to 1000 pg/mL for IL-6 measurement with a limit of detection (LOD) of 0.076 pg/mL for the electrochemical approach and 0.10 pg/mL for the optical LSPR approach. Furthermore, the performance of the dual-mode sensing microcatheter was successfully evaluated in buffer, artificial serum, skin-gel, and human serum samples with co-interferents in the clinical range.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"433 ","pages":"Article 137514"},"PeriodicalIF":8.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143548680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A portable ascorbic acid touch-in personal glucose meter based on enzyme inhibition cascade reaction for on-site rapid detection of organophosphorus pesticides

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-05 DOI: 10.1016/j.snb.2025.137576

Jing Yang , Yaqin Zhang , Xingbo Shi, Yan Lv

{"title":"A portable ascorbic acid touch-in personal glucose meter based on enzyme inhibition cascade reaction for on-site rapid detection of organophosphorus pesticides","authors":"Jing Yang , Yaqin Zhang , Xingbo Shi, Yan Lv","doi":"10.1016/j.snb.2025.137576","DOIUrl":"10.1016/j.snb.2025.137576","url":null,"abstract":"<div><div>Rapid and portable detection technology of organophosphorus pesticides (OPs) is urgently needed to ensure food and environmental safety. Herein, a simple and portable method for in-field detection of OPs was established based on an ascorbic acid (AA) touch-in personal blood glucose meter (PGM) triggered by the inhibition of alkaline phosphatase (ALP). Particularly, AA was first used as a bridge to construct a cascade strategy ALP-AA-[Fe(CN)<sub>6</sub>]<sup>3-</sup> for PGM readout, as it has been proven to trigger stronger PGM readings due to its strong reducibility and double electron generation. In the presence of OPs, the enzymatic activity of ALP was inhibited to produce less AA, resulting in a weakened reduction of [Fe(CN)<sub>6</sub>]<sup>3-</sup> on the test strip and a decrease of the electrochemical signal. The PGM analysis platform showed a good linear response at concentrations of 10–40 μg L<sup>−1</sup> of dimethoate, with a detection limit of 7.55 μg L<sup>−1</sup>, and its practicability and reliability were verified by the recovery results in vegetables and fruits. With the advantages of simplicity and portability, this method can complete the entire field detection process in 20 min. Hence, this work provides a promising candidate method for the rapid on-site detection of OPs in food and the environment.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"433 ","pages":"Article 137576"},"PeriodicalIF":8.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fluorescent optical fiber sensor for real-time, portable detection of alkaline phosphatase activity

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-05 DOI: 10.1016/j.snb.2025.137568

Dan Li , Yutao Shen , Na Li , Xiaolong Li , Mao Li , Zijia Huang , Yong Zhao

{"title":"A fluorescent optical fiber sensor for real-time, portable detection of alkaline phosphatase activity","authors":"Dan Li , Yutao Shen , Na Li , Xiaolong Li , Mao Li , Zijia Huang , Yong Zhao","doi":"10.1016/j.snb.2025.137568","DOIUrl":"10.1016/j.snb.2025.137568","url":null,"abstract":"<div><div>Alkaline phosphatase (ALP) is ubiquitous in mammalian tissues, facilitating the dephosphorylation of diverse biomolecules. As a key biomarker, ALP activity monitoring is of great significance for clinical research and disease diagnosis. Here, a sensitive and portable fluorescent fiber optic sensor based on silicon quantum dots (SiQDs) was developed for detecting ALP activity. Green-fluorescent SiQDs were synthesized using a one-pot hydrothermal method. A tapered optical fiber was facilely modified with SiQDs through in situ hydrogel polymerization reaction. The hydrogel-based optical fiber ALP sensor was meticulously designed by incorporating ALP-triggered cascade reaction. ALP catalyzes the substrate hydrolysis to produce ascorbic acid (AA), which then triggers a chromogenic redox reaction between AA and Cu(II)-neocuproine chelate to form highly colored Cu(I)-chelate, effectively quenching the fluorescence of the SiQDs due to inner filter effect. This method provides a linear detection of ALP activity within the range of 0.02–2.0 U/L. The sensor was applied to ALP activity detection in real serum samples, demonstrating its practicability and reliability. This sensor offers the advantages of high sensitivity (a detection limit of 0.015 U/L), exceptional selectivity, good practicability and simplified fabrication, presenting a portable and efficient technique for on-line ultrasensitive detection of ALP activity in biological samples. It provides a new perspective for developing high-performance optical fiber sensors, with significant practical value for applications in health monitoring, clinical diagnostics, and emergency analysis.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"433 ","pages":"Article 137568"},"PeriodicalIF":8.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143547025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of ultrafast-walking stochastic 3D dual-DNA walker nanoprobes for highly sensitive fluorescence detection of extracellular vesicles for cancer diagnosis

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-05 DOI: 10.1016/j.snb.2025.137578

Qingyi Liu , Qiongdan Zhang , Ruiyue Zhang , Zichen Wen , Zhijian Yao , Bin Li , Zheng Yang , Jinhui Hu , Wei Wang , Huizhen Wang , Caiyun Peng

{"title":"Construction of ultrafast-walking stochastic 3D dual-DNA walker nanoprobes for highly sensitive fluorescence detection of extracellular vesicles for cancer diagnosis","authors":"Qingyi Liu , Qiongdan Zhang , Ruiyue Zhang , Zichen Wen , Zhijian Yao , Bin Li , Zheng Yang , Jinhui Hu , Wei Wang , Huizhen Wang , Caiyun Peng","doi":"10.1016/j.snb.2025.137578","DOIUrl":"10.1016/j.snb.2025.137578","url":null,"abstract":"<div><div>Extracellular vesicles (EVs) serve as a promising biomarker for the early screening and monitoring of cancer through non-invasive testing. However, achieving rapid and sensitive detection of tumor-derived extracellular vesicles (TEVs) remains a significant challenge. In this study, we report an ultrafast-walking stochastic three-dimensional (3D) dual-DNA walker nanoprobes (D-DWN)-based fluorescence biosensor for the detection of EVs. The D-DWN facilitates autonomous movement on AuNP at high speeds, powered by Exo III, which results in the release of fluorophores that act as an analytical signal for the detection of target EVs. Compared to traditional DNA walkers, our proposed D-DWN demonstrates superior reaction kinetics, achieving saturation within 20 min. This advancement enables highly sensitive detection of EVs, with a linear response range from 8 × 10<sup>5</sup> to 1.28 × 10<sup>7</sup> particles/μL and a limit of detection of 1.18 particles/μL. Furthermore, clinical samples can be analyzed using the D-DWN to differentiate patients with cancer from healthy individuals. This work is anticipated to provide an effective tool for the accurate detection of EVs, offering potential for early cancer diagnosis and postoperative response prediction.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"433 ","pages":"Article 137578"},"PeriodicalIF":8.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0