Sensors and Actuators B: Chemical最新文献_第6页

Construction of a cellulose-based dual-responsive fluorescent probe for simultaneous pH and HClO detection and imaging in arthritic mice 基于纤维素的双响应荧光探针的构建，用于同时检测关节炎小鼠的pH和HClO

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-10-10 DOI: 10.1016/j.snb.2025.138940

Wenchan Dong , Xia Xue , Hongkun Yang , Keyin Liu , Mingguang Ren , Xiaohui Wang , Fangong Kong

{"title":"Construction of a cellulose-based dual-responsive fluorescent probe for simultaneous pH and HClO detection and imaging in arthritic mice","authors":"Wenchan Dong , Xia Xue , Hongkun Yang , Keyin Liu , Mingguang Ren , Xiaohui Wang , Fangong Kong","doi":"10.1016/j.snb.2025.138940","DOIUrl":"10.1016/j.snb.2025.138940","url":null,"abstract":"<div><div>pH and hypochlorous acid (HClO) are important active factors in living organisms and play a crucial role in the occurrence and progression of arthritis. Herein, a novel cellulose-based dual-responsive fluorescent probe was developed for highly sensitive and selective detect of pH and HClO. In the design, 4-(2-aminoethylamino) −1,8-naphthalimide and modified Nile red were grafted together onto a toluenesulfonyl actived cellulose to fabricate the cellulose-based probe. 4-(2-aminoethylamino) −1,8-naphthalimide was supplied as the pH-responsive part, and Nile red derivatives supplied as HClO-reponsive part, respectively. Upon decreasing solution pH, the cellulose-based probe exhibited 5-fold fluorescence enhancement at 560 nm. While, increasing of HClO concentrations resulted in remarkable fluorescence enhancement at 670 nm. The limit of detection (LOD) and limit of quantification (LOQ) for HClO were determined to be approximately 0.186 μM and 0.621 μM, respectively. The detection mechanism was explored by DFT calculation and HRMS analysis. Then it was successfully applied to detect pH and HClO within cells and zebrafish, and further validated in a mouse model of arthritis. The cellulose-based probe had demonstrated good biocompatibility, sensitivity, and prolonged fluorescence imaging capabilities (fluorescence stable for 3 h, over 50 % signal at 12 h). These results suggest the cellulose-based probe can potentially be used as a real-time monitoring tool for assessing clinical arthritis.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138940"},"PeriodicalIF":3.7,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145255181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulating porous In2O3 nanonest like structures by Pr-Ag dopant ratio for enhanced methanol gas sensing properties 用Pr-Ag掺杂比调制多孔In2O3纳米样结构增强甲醇气敏性能

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-10-10 DOI: 10.1016/j.snb.2025.138937

Kairui Liu , Lei Cao , Jianing Liu , Lihao Lv , Chuantao Zhang , Senlin Li , Lingmin Yu , Xinhui Fan , Puhong Wang , Jingfeng Wang , Lei Guo , Zhijun Ding

{"title":"Modulating porous In2O3 nanonest like structures by Pr-Ag dopant ratio for enhanced methanol gas sensing properties","authors":"Kairui Liu , Lei Cao , Jianing Liu , Lihao Lv , Chuantao Zhang , Senlin Li , Lingmin Yu , Xinhui Fan , Puhong Wang , Jingfeng Wang , Lei Guo , Zhijun Ding","doi":"10.1016/j.snb.2025.138937","DOIUrl":"10.1016/j.snb.2025.138937","url":null,"abstract":"<div><div>In current research, In<sub>2</sub>O<sub>3</sub> is widely used as n-type semiconductor gas sensing materials for detecting toxic and hazardous gases. However, In<sub>2</sub>O<sub>3</sub> sensors still have disadvantages such as high working temperature and low response value. To address these challenges, Pr-Ag co-doped porous nanonest like In<sub>2</sub>O<sub>3</sub> was prepared by adjusting the doping molar ratio of Pr and Ag using a hydrothermal method. This sensor exhibits excellent methanol gas sensing properties, including a high gas response value of 21.6 (10 ppm), fast gas adsorption and desorption equilibrium (10 s/9 s), and a low operating temperature (175°C). The enhanced sensing mechanism was clarified through a combination of experiments and first-principles calculations. The results showed that after the co-doping of Pr and Ag, there was a higher adsorption energy, a narrower band gap, and a larger specific surface area, which significantly improved the gas sensing performance. The present work work open up a path for more accurate and better detection of methanol concentrations in industrial environments.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138937"},"PeriodicalIF":3.7,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145255182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-mode biosensor based on cascade CHA-HCR amplification and multilayer core-satellite nanostructures for highly sensitive microRNA detection 基于级联CHA-HCR扩增和多层核心-卫星纳米结构的高灵敏度微rna检测双模生物传感器

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-10-10 DOI: 10.1016/j.snb.2025.138939

Baozheng Wang , Zixuan Ding , Wen Li , Hong Wang, Yingwei Zhang

{"title":"Dual-mode biosensor based on cascade CHA-HCR amplification and multilayer core-satellite nanostructures for highly sensitive microRNA detection","authors":"Baozheng Wang , Zixuan Ding , Wen Li , Hong Wang, Yingwei Zhang","doi":"10.1016/j.snb.2025.138939","DOIUrl":"10.1016/j.snb.2025.138939","url":null,"abstract":"<div><div>To achieve highly sensitive and reliable detection of pivotal cancer diagnostic biomarkers like miRNA, and to develop a novel core-satellite assembly strategy for SERS sensing platforms with high \"hot spot\" density, this study introduces an innovative approach. We integrate enzyme-free, isothermal Catalytic Hairpin Assembly (CHA) and Hybridization Chain Reaction (HCR) in a cascaded manner to construct multi-layered \"core-satellite\" nanostructures. CHA enables specific target recognition and initial signal amplification, while subsequent cascaded HCR generates long dsDNA scaffolds, facilitating the assembly of multiple satellite nanoparticle layers around a central core. This design significantly increases the number and density of SERS \"hot spots,\" leading to substantially enhanced signals. The strategy was successfully applied for the highly sensitive detection of miRNA-21. Furthermore, a dual-modal biosensing platform combining fluorescence and SERS outputs was developed, improving detection reliability and accuracy. This work presents a robust methodology for precise miRNA analysis and advances the development of high-performance, multi-layered core-satellite nanostructures for broader biosensing applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138939"},"PeriodicalIF":3.7,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145255183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A surface-enhanced Raman scattering and fluorescence dual-mode aptasensor based on magnetic plasmonic core-shell nanostructures and N-doped carbon dots for aflatoxin B1 detection 基于磁等离子体核壳纳米结构和n掺杂碳点的表面增强拉曼散射和荧光双模适体传感器用于黄曲霉毒素B1检测

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-10-09 DOI: 10.1016/j.snb.2025.138928

Zhihui Wu , Xiangzhi Li , Yucong Ma , Fangze Qin , Haoyang He , Hongbin Pu , Shi Gang Liu

{"title":"A surface-enhanced Raman scattering and fluorescence dual-mode aptasensor based on magnetic plasmonic core-shell nanostructures and N-doped carbon dots for aflatoxin B1 detection","authors":"Zhihui Wu , Xiangzhi Li , Yucong Ma , Fangze Qin , Haoyang He , Hongbin Pu , Shi Gang Liu","doi":"10.1016/j.snb.2025.138928","DOIUrl":"10.1016/j.snb.2025.138928","url":null,"abstract":"<div><div>Aflatoxin B1 (AFB1) contamination in foods threatens human health worldwide, and accurate and rapid detection of AFB1 is essential. Herein, a surface-enhanced Raman scattering (SERS) and fluorescence dual-mode aptasensor is constructed for sensitive detection of AFB1 based on ZnFe<sub>2</sub>O<sub>4</sub> magnetic nanoparticle@gold nanoparticles-silver shell nanostructures (MNP@AuNPs-Ag) and fluorescent N-doped carbon dots (N-CDs). Specifically, Raman beacon molecule cyanine 3-modified aptamer (Apt-Cy3) is coupled on the MNP@AuNPs-Ag surface, and complementary DNA (cDNA) is modified on the N-CDs surface (N-CDs-cDNA). In the absence of AFB1, thanks to the DNA hybridization, low fluorescence for the supernatant and low SERS for the precipitate appear after magnetic separation. However, in the presence of AFB1, most of N-CDs-cDNA are free as the aptamer preferentially binds to AFB1, resulting in a strong fluorescent signal in the supernatant, while the change in the secondary structure of the aptamer shortens the spatial distance between Cy3 and MNP@AuNPs-Ag, producing a strong SERS signal. Based on the principle, the dual-mode aptasensor is developed and quantifies AFB1 using SERS and fluorescence signals with the low detection limits of 0.88 and 9.74 pg/mL, respectively. What's more, the dual-mode aptasensor is applied to the detection of AFB1 in real food samples successfully. This work exploits magnetic plasmonic core-shell nanostructures and fluorescent N-CDs to obtain fluorescence and SERS signals from the supernatant and the precipitate respectively after magnetic separation. The strategy not only provides a robust method for detection of AFB1 but also offers new insights for the design of dual-mode aptasensor.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"138928"},"PeriodicalIF":3.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145242287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-signal amplification via high-entropy alloy nanoparticle catalysis and target DNA recycling for ultrasensitive HPV-16/18 detection in human serum 高熵合金纳米颗粒催化双信号扩增和靶DNA循环用于人血清HPV-16/18的超灵敏检测

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-10-09 DOI: 10.1016/j.snb.2025.138927

Wang Miao

{"title":"Dual-signal amplification via high-entropy alloy nanoparticle catalysis and target DNA recycling for ultrasensitive HPV-16/18 detection in human serum","authors":"Wang Miao","doi":"10.1016/j.snb.2025.138927","DOIUrl":"10.1016/j.snb.2025.138927","url":null,"abstract":"<div><div>Rapid identification of HPV-16/18 genotypes is critical for cervical cancer prevention, yet remains clinically inaccessible due to inadequate diagnostic sensitivity and specificity. The paper presents an electrochemical DNA biosensor achieving detection of genotypes through an innovative dual-signal amplification cascade. First, an exceptionally active AlCrMnZnAu<sub>0.1</sub> high-entropy alloy was engineered via coordination-driven approach using folic acid and histidine-functionalized boron and nitrogen-doped graphene quantum dot. Synergistic atomic orbital hybridization and tailored support endow unprecedented stability, optimized electron structure and remarkable catalytic activity—outperforming benchmark gold nanoparticle by 6.8-fold. Second, triblock poly-adenine probe (H1) anchored to gold electrode selectively captures HPV-16 or HPV-18 targets. Binding initiates the unfolding of secondary structures: H2 (tagged with AlCrMnZnAu<sub>0.1</sub>/thionine) for HPV-16, and H3 (tagged with AlCrMnZnAu<sub>0.1</sub>/ferrocene) for HPV-18. Crucially, target DNA is regenerated through toehold-mediated strand displacement, enabling geometric signal amplification. Concurrently, AlCrMnZnAu<sub>0.1</sub> intensifies electrochemical responses by catalytically boosting thionine and ferrocene redox reactions. This orchestrated dual-amplification strategy delivers unmatched specificity and sensitivity for HPV-16/18. The platform exhibits an exceptionally broad linear response (10⁻¹⁸ to 10⁻¹³ M) for both targets and achieves ultralow detection limit of 2.9 × 10⁻¹ ⁹ M, representing a transformative advancement over existing state-of-the-art diagnostic sensors for point-of-care deployment.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138927"},"PeriodicalIF":3.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145242286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light-controlled one-pot RPA-CRISPR/Cas12a biosensor for MRSA detection based on tailed crRNA 基于尾端crRNA的MRSA检测光控单锅式rna - crispr /Cas12a生物传感器

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-10-09 DOI: 10.1016/j.snb.2025.138925

Zhou Yu , Yan Man , Weilin Li , Cong Shi , Peng Xiao , Qinghai Zhang

{"title":"Light-controlled one-pot RPA-CRISPR/Cas12a biosensor for MRSA detection based on tailed crRNA","authors":"Zhou Yu , Yan Man , Weilin Li , Cong Shi , Peng Xiao , Qinghai Zhang","doi":"10.1016/j.snb.2025.138925","DOIUrl":"10.1016/j.snb.2025.138925","url":null,"abstract":"<div><div>Methicillin-resistant Staphylococcus aureus (MRSA) poses a severe global health threat due to its antibiotic resistance and high mortality rates. Herein, we developed a novel light-controlled one-pot RPA-CRISPR/Cas12a sensing system for rapid, specific, and cost-effective MRSA detection. The sensing system features a \"tailed crRNA\" containing a photocleavable linker (PC-linker), which blocks target recognition through a self-forming hairpin structure, maintaining the CRISPR/Cas system in an inactive state during RPA. Upon ultraviolet irradiation, the PC-linker is cleaved, restoring crRNA activity and enabling one-pot integration of amplification and detection within a single closed reaction tube. The traditional two-step RPA-CRISPR/Cas12a method demonstrated a linear detection range of 8.9 × 10⁰ – 8.9 × 10⁶ CFU/mL with a limit of detection (LOD) of 6.3 CFU/mL, while the light-controlled one-pot RPA-CRISPR/Cas12a system achieved comparable sensitivity (LOD: 34.7 CFU/mL) and specificity with no cross-reactivity against non-target pathogens. Both systems exhibited excellent recovery rates (87.1–110.2 %) and reproducibility (RSD ≤ 6.22 %) in complex food matrices (lettuce, milk, salmon, sauce beef). This light-controlled approach significantly reduces cross-contamination risks while simplifying workflows, providing a sensitive, reliable, and user-friendly platform for point-of-need MRSA detection in food safety and clinical diagnostics.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138925"},"PeriodicalIF":3.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Baijiu flavor types by MEMS gas sensor based on temperature programmed desorption method 基于程序升温解吸法的MEMS气体传感器识别白酒风味类型

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-10-09 DOI: 10.1016/j.snb.2025.138921

Renjun Si , Hongze Jiang , Jinbo Yang , Renminxi Ou , Shaofeng Chen , Xin Guo , Shunping Zhang

引用次数: 0

Colorimetric sensing platform based on persulfate activation on fluorine-modified metal-organic frameworks for fast and selective detection of perfluorooctane sulfonic acid 基于过硫酸盐活化氟修饰金属-有机骨架的比色传感平台快速选择性检测全氟辛烷磺酸

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-10-09 DOI: 10.1016/j.snb.2025.138926

Qi An , Yi Tan , Xinze Du , Heyun Fu , Pedro J.J. Alvarez , Xiaolei Qu

{"title":"Colorimetric sensing platform based on persulfate activation on fluorine-modified metal-organic frameworks for fast and selective detection of perfluorooctane sulfonic acid","authors":"Qi An , Yi Tan , Xinze Du , Heyun Fu , Pedro J.J. Alvarez , Xiaolei Qu","doi":"10.1016/j.snb.2025.138926","DOIUrl":"10.1016/j.snb.2025.138926","url":null,"abstract":"<div><div>Widespread environmental pollution by highly-persistent and toxic perfluorooctane sulfonic acid (PFOS) underscores the need for a low-cost, high-throughput detection method to assess exposure risks and regulatory compliance. Herein, we developed a novel colorimetric sensing platform, based on peroxymonosulfate (PMS) activation by fluorine-modified metal-organic frameworks (MOFs), for sensitive and selective PFOS detection. Specifically, PCN-222(Fe) and its fluorinated counterpart, PCN-222(Fe)-3F, efficiently activate PMS, leading to the oxidation of the chromogenic substrate 3,3’,5,5’-tetramethylbenzidine (TMB) into its blue oxidized form. The oxidation is primarily mediated by high-valent iron-oxo species rather than free radicals, offering high selectivity. PFOS binds to the catalytic sites of MOFs, effectively inhibiting TMB oxidation. PCN-222(Fe)-3F exhibits superior PFOS selectivity compared to PCN-222(Fe) owing to specific fluorine-fluorine interactions. The developed colorimetric sensor has a low detection limit of 12.6 nM, fast detection within 30 min, and a broad linear range. It exhibits excellent selectivity against common interferents and performs reliably in real water sample analysis. The reported colorimetric sensor based on persulfate activation offers a promising approach for the development of low-cost, high-throughput, and on-site screening of per-and perfluorinated alkyl substances.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138926"},"PeriodicalIF":3.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145255184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amplified broad-spectrum detection of tetracyclines in surface water using CdTe@PVP@ZIF-8-accelerated cation exchange reactions and TMSDR-driven self-protective DNAzyme 利用CdTe@PVP@ zif -8加速阳离子交换反应和tmsdr驱动的自保护DNAzyme对地表水中四环素类药物的放大广谱检测

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-10-09 DOI: 10.1016/j.snb.2025.138914

Shuangyu Meng , Bowen Li , Chunyuan Huo , Wentong Yu , Ziyan Zhao , Runlong Ma , Zhiqiang Guo , Xinru Ren , Haiping Zhang , Jiadong Huang , Yu Wang , Su Liu

{"title":"Amplified broad-spectrum detection of tetracyclines in surface water using CdTe@PVP@ZIF-8-accelerated cation exchange reactions and TMSDR-driven self-protective DNAzyme","authors":"Shuangyu Meng , Bowen Li , Chunyuan Huo , Wentong Yu , Ziyan Zhao , Runlong Ma , Zhiqiang Guo , Xinru Ren , Haiping Zhang , Jiadong Huang , Yu Wang , Su Liu","doi":"10.1016/j.snb.2025.138914","DOIUrl":"10.1016/j.snb.2025.138914","url":null,"abstract":"<div><div>Tetracyclines (TCs) are important in animal husbandry, aquaculture and medical fields, which pose environmental residues and potential health risks. Consequently, the detection of TCs is important for environmental safety. However, traditional detection methods are time-consuming and laborious, and the sensitivity needs to be improved. Accordingly, it is necessary to establish a highly specific and accurate approach for the detection of TCs. Herein, based on CdTe@PVP@ZIF-8 material, a fluorescent biosensor combined with self-locking allosteric hypersensitive DNAzyme (SAH-Dz) was developed for the broad-spectrum detection of TCs in surface water. During the detection process, TCs first bind to the aptamer to activate the toehold-mediated strand displacement reaction (TMSDR), which generates a large amount of P. Subsequently, the binding region of SAH-Dz can change the self-locking conformation by complementary pairing with the base of P, release the DNAzyme binding arm, bind to the substrate chain H, cleave the rA site in the presence of Mg<sup>2</sup><sup>+</sup> , and release Ag<sup>+</sup> diffusing into the porous ZIF-8. Cation exchange reactions (CER) occur between Ag<sup>+</sup> and CdTe QDs, inducing fluorescence quenching. The extent of the fluorescence signal's weakening is utilized for the quantitative determination of TCs concentrations. Under optimized conditions, the detection limit of this method for TCs reached 0.0987 nM. This method can achieve sensitive, specific and broad-spectrum detection of four TCs, and is suitable for the identification of antibiotics in surface water.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138914"},"PeriodicalIF":3.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145255185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid, sensitive and wide-spectrum colourimetric detection of viable Staphylococcus aureus in food using phage cocktail 利用噬菌体鸡尾酒快速、灵敏、广谱比色法检测食品中活菌金黄色葡萄球菌

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-10-09 DOI: 10.1016/j.snb.2025.138910

Wenyuan Zhou , Wenjuan Li , Aiping Deng , Yelin Han , Yajie Li , Yajun Gao , Qin Hu , Guoqiang Zhu , Zhenquan Yang

{"title":"Rapid, sensitive and wide-spectrum colourimetric detection of viable Staphylococcus aureus in food using phage cocktail","authors":"Wenyuan Zhou , Wenjuan Li , Aiping Deng , Yelin Han , Yajie Li , Yajun Gao , Qin Hu , Guoqiang Zhu , Zhenquan Yang","doi":"10.1016/j.snb.2025.138910","DOIUrl":"10.1016/j.snb.2025.138910","url":null,"abstract":"<div><div>Although bacteriophages (phages) are ideal biorecognition elements employed for <em>Staphylococcus aureus</em> detection, existing single-phage-based approaches are limited by the specific affinity of phages and false-negative results. To develop an improved phage-based colourimetric detection system and investigate its detection mechanism, we prepared a phage cocktail composed of three <em>S. aureus</em> phages (SapYZU10, SapYZU11, and SapYZUM13), immobilised it on Co-Mn nanozymes, and applied it to <em>S. aureus</em> for evaluation. The phage cocktail@Co-Mn demonstrated a sensitive, specific, and fast colourimetric evaluation of live <em>S. aureus</em> counts in approximately 17 min, with a detection limit of 92 CFU/mL. Moreover, the phage cocktail@Co-Mn was successfully used to detect <em>S. aureus</em> in food samples, with recovery rates ranging from 92.21 % to 105.56 %. The phage cocktail@Co-Mn also exhibited resilience to changes in pH (3–9), five food additives, and NaCl concentration (2 %–10 %). The detection mechanism of the phage cocktail@Co-Mn involves blockage of the active sites of the nanozyme producing O<sub>2</sub><sup>•–</sup>. Notably, owing to the synergistic recognition spectra of the three phages in the cocktail, the recognition rate for the strains was a remarkable 100 %, compared with the much lower recognition rates of individual phages (between 74.7 % and 76.9 %). The recognition mechanism involves adsorption between phage tail fibres and <em>S. aureus</em> hosts. This pioneering study revealed that the phage cocktail is an effective biorecognition element suitable for the rapid, sensitive, specific, reliable, and colourimetric detection of viable <em>S. aureus</em> strains in the food industry, providing innovative directions for the phage-based detection methods.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138910"},"PeriodicalIF":3.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145242288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0