Sensors and Actuators B: Chemical最新文献_第6页

A triple-targeting and viscosity-sensitive fluorescence probe for visualization of liver injury and tumor models

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-17 DOI: 10.1016/j.snb.2025.137614

Peng Lei , Pengjia Wei , Chuan Dong , Shaomin Shuang , Minglu Li

{"title":"A triple-targeting and viscosity-sensitive fluorescence probe for visualization of liver injury and tumor models","authors":"Peng Lei , Pengjia Wei , Chuan Dong , Shaomin Shuang , Minglu Li","doi":"10.1016/j.snb.2025.137614","DOIUrl":"10.1016/j.snb.2025.137614","url":null,"abstract":"<div><div>Targeting multiple organelles can provide a more comprehensive understanding of cellular function and metabolism. However, the current research reveals a significant deficiency in the availability of multi-organelle fluorescent probes, which limits ability to study the complex interplay between these organelles in real-time. Furthermore, the development of a multi-organelle viscosity fluorescent probe that is capable of visualizing multiple disease models (liver injury and tumor) poses severe deficiency and remains a great challenge. Herein, for the first time, we developed a novel triple-targeting fluorescence probe (membranes, mitochondria and nuclei, named MMN), which can initially accumulate on the cell membranes, then migrate to mitochondria, and ultimately binds to the nuclei through DNA interaction. MMN consisted of a donor-π-acceptor (D-π-A) structure and extended double bonds bridging rotor, which exhibited sensitive response to viscosity and strong fluorescence intensity. The twisted intramolecular charge transfer (TICT) of MMN was suppressed with an increase in viscosity, accompanied by a significant enhancement of red fluorescence signal with emission wavelength of 585 nm. Cascade imaging of mitochondria and nuclei with MMN allowed real-time monitoring and visualization of mitochondrial status within live cells. Using MMN, we have demonstrated the visualization of liver injury and tumor via mitochondrial viscosity imaging. Therefore, MMN holds significance for studies related to mitochondrial injury and the prevention and treatment of associated diseases.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"434 ","pages":"Article 137614"},"PeriodicalIF":8.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143635644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel one-pot RPA-CRISPR/Cas13a diagnostic system for rapid and specific detection of HAdV infections

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-17 DOI: 10.1016/j.snb.2025.137611

Yuanyuan Li , Pan Liu , Hongyu Zhao , Lihua Liang , Minchai Chen , Xiaohan Yang

{"title":"A novel one-pot RPA-CRISPR/Cas13a diagnostic system for rapid and specific detection of HAdV infections","authors":"Yuanyuan Li , Pan Liu , Hongyu Zhao , Lihua Liang , Minchai Chen , Xiaohan Yang","doi":"10.1016/j.snb.2025.137611","DOIUrl":"10.1016/j.snb.2025.137611","url":null,"abstract":"<div><div>Human adenovirus (HAdV) infections are prevalent and can lead to severe respiratory, ocular, and gastrointestinal diseases, thereby posing a significant public health risk, particularly among children and immunocompromised individuals. The genetic diversity of HAdV complicates rapid and accurate detection, highlighting the necessity for efficient and straightforward diagnostic methods. This research introduces a convenient and rapid one-pot RPA-CRISPR/Cas13a diagnostic system that is both sensitive and specific for the identification of HAdV DNA. The system incorporates two detection modalities: RPA-CRISPR/Cas13a-Flu for fluorescence-based detection and RPA-CRISPR/Cas13a-LFS for visual detection. The sensitivity of the RPA-CRISPR/Cas13a-Flu method was measured at 5 copies per reaction, while the RPA-CRISPR/Cas13a-LFS demonstrated a sensitivity of 50 copies per reaction, with both methods achieving 100 % specificity. Validation against 175 clinical samples indicated that the RPA-CRISPR/Cas13a-Flu had a sensitivity of 95.95 % (95 % CI 88.75–98.61), specificity of 100 % (96.34–100), accuracy of 98.29 % (95.08–99.42), and a Kappa coefficient of 0.965 when compared to traditional qPCR methods. The RPA-CRISPR/Cas13a-LFS exhibited 81.08 % (70.71–88.38) sensitivity, 100 % (96.34–100) specificity, 92.00 % (87.02–95.17) accuracy, and a Kappa coefficient of 0.832. Our developed one-pot RPA-CRISPR/Cas13a system offers a rapid, user-friendly, and visually interpretable alternative to PCR for on-site HAdV detection, with potential applications in early diagnosis, subtyping, drug resistance detection, and portable monitoring of various infectious pathogens.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137611"},"PeriodicalIF":8.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel method for monitoring residual hexane in refined oils using semiconducting metal oxide-based gas sensors

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-17 DOI: 10.1016/j.snb.2025.137616

Asia Kalinichenko, André Sackmann, Benjamin Junker, Udo Weimar, Nicolae Bârsan

{"title":"Novel method for monitoring residual hexane in refined oils using semiconducting metal oxide-based gas sensors","authors":"Asia Kalinichenko, André Sackmann, Benjamin Junker, Udo Weimar, Nicolae Bârsan","doi":"10.1016/j.snb.2025.137616","DOIUrl":"10.1016/j.snb.2025.137616","url":null,"abstract":"<div><div>A simple and rapid method for the determination of residual hexane content in refined oils has been developed. It is based on the direct analysis of oil headspace using SMOX-based gas sensors, providing a detection limit of 0.3 mg·kg<sup>−1</sup> for residual hexane. The accuracy and precision of the proposed method have been proven for the working range of 0.5–33 mg·kg<sup>−1</sup>, using GC-MS as a reference method meeting the requirement of the European Union regulations. The robustness of the proposed method was confirmed by investigating possible variations in relative humidity under out-of-laboratory conditions. Moreover, it was possible to determine ageing equations for sensitivity correction during sensor usage, which reduce the need for sensor recalibration. The ageing equations of sensors and the determination of the relationship between the concentration of hexane injected in the oil and extracted in the headspace were determined using a computer-controlled gas mixing system. The oil matrix effect was found to be negligible for oils meeting the required quality parameters. The developed sensor-based method allows for monitoring residual hexane in edible oils, both during the oil extraction process and in the safety control of the final product. It provides an alternative to the currently used ones, such as gas chromatography and the flash point procedure, which are either complex or have sensitivity limitations.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"434 ","pages":"Article 137616"},"PeriodicalIF":8.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143635642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A one-pot synthesized dual-emission MOFs-based ratiometric fluorescent sensor for hypersensitive and portable detection of griseofulvin

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-17 DOI: 10.1016/j.snb.2025.137613

Guang-Ming Bao , Di-Jian Zhang , Wei Li , Yi-Fan Xia , Zhi-Qiang Cai , Akimana Sandra , Hou-Qun Yuan , Peiyao Chen , Jian-Ping Huang , Le Fang

{"title":"A one-pot synthesized dual-emission MOFs-based ratiometric fluorescent sensor for hypersensitive and portable detection of griseofulvin","authors":"Guang-Ming Bao , Di-Jian Zhang , Wei Li , Yi-Fan Xia , Zhi-Qiang Cai , Akimana Sandra , Hou-Qun Yuan , Peiyao Chen , Jian-Ping Huang , Le Fang","doi":"10.1016/j.snb.2025.137613","DOIUrl":"10.1016/j.snb.2025.137613","url":null,"abstract":"<div><div>Griseofulvin (GRF) is a non-polyene antifungal antibiotic commonly used in agriculture for fungal disease control. Improper exposure to GRF can pose significant health risks to human. Despite its widespread use, there is a lack of simple, low-cost, highly selective and sensitive methods for the detection of GRF. In this regard, a fluorescent and colorimetric sensor was developed through a one pot reaction by encapsulating vitamin B<sub>2</sub> (VB<sub>2</sub>) in a metal organic framework material UiO-66-NH<sub>2</sub>. In the presence of GRF, the fluorescence intensity at 435 nm increased, while the fluorescence intensity at 520 nm decreased, resulting in a ratiometric fluorescence change. And the ratio of fluorescence intensities at these two wavelengths exhibited a strong linear relationship with GRF concentration. The UiO-66-NH<sub>2</sub>@VB<sub>2</sub> showed excellent GRF response with high selectivity, low LOD (27.17 nM) and quick response time (less than 1 min), and was successfully applied to detect GRF in real samples of ambient water and milk with good recoveries. Furthermore, a paper-based sensor of UiO-66-NH<sub>2</sub>@VB<sub>2</sub> was fabricated for GRF detection under UV light, and the RGB color change could be recognized by a smartphone. This simple and efficient method allows for on-site, rapid, and quantitative detection of GRF in ambient water and milk, demonstrating its promising application for GRF detection in food safety and environmental monitoring.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"434 ","pages":"Article 137613"},"PeriodicalIF":8.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of NO2 sensing of ZnO by irradiating with high-energy Ni ions

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-17 DOI: 10.1016/j.snb.2025.137615

Wansik Oum , Ali Mirzaei , Ka Yoon Shin , Eun Bi Kim , Sungjoon Moon , Sang Sub Kim , Hyoun Woo Kim

{"title":"Enhancement of NO2 sensing of ZnO by irradiating with high-energy Ni ions","authors":"Wansik Oum , Ali Mirzaei , Ka Yoon Shin , Eun Bi Kim , Sungjoon Moon , Sang Sub Kim , Hyoun Woo Kim","doi":"10.1016/j.snb.2025.137615","DOIUrl":"10.1016/j.snb.2025.137615","url":null,"abstract":"<div><div>Although high-energy irradiation is a feasible route to amplify sensing characteristics, only a few studies have focused on this technique. In this study, high-energy (100 keV) Ni<sup>+</sup> ions were irradiated onto ZnO nanoparticles at three varying ion doses (10<sup>14</sup>, 10<sup>15</sup>, and 10<sup>16</sup> ions/cm<sup>2</sup>) for detecting NO<sub>2</sub>. The characterization results showed that the irradiated samples contain more oxygen vacancies than the pristine sample; irradiation at the lowest dosage produces the highest number of oxygen vacancies. At 250 °C, ZnO irradiated with the lowest dose demonstrates the largest response of 120.8–10 ppm NO<sub>2</sub> gas. Moreover, it exhibited long-term stability, high selectivity, repeatability, and reproducibility. The optimized sensor showed a smaller decrease in its response under humid environments compared to the pristine sensor. This enhanced performance was ascribed to the formation of large numbers of oxygen vacancies through Ni<sup>+</sup> irradiation. This study emphasizes the promising role of Ni<sup>+</sup> irradiation and the importance of optimizing its dosage to achieve the highest sensing output.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137615"},"PeriodicalIF":8.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in p-type delafossite ABO2 based chemiresistive gas sensors

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-16 DOI: 10.1016/j.snb.2025.137606

Sunil Kumar , Zanhong Deng , Siyuan Liu , Gang Meng

引用次数: 0

High-sensitivity biosensors based on reflection-type guided-mode-resonance secondary grating metasurfaces

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-16 DOI: 10.1016/j.snb.2025.137609

Lijun Ma , Liye Li , Bingrui Wang , Yusa Chen , Shuai Wang , Yunhao Cao , Hongshun Sun , Yongchen Ma , Xiaolong Rao , Long Rong , Wengang Wu

{"title":"High-sensitivity biosensors based on reflection-type guided-mode-resonance secondary grating metasurfaces","authors":"Lijun Ma , Liye Li , Bingrui Wang , Yusa Chen , Shuai Wang , Yunhao Cao , Hongshun Sun , Yongchen Ma , Xiaolong Rao , Long Rong , Wengang Wu","doi":"10.1016/j.snb.2025.137609","DOIUrl":"10.1016/j.snb.2025.137609","url":null,"abstract":"<div><div>Subwavelength dielectric grating waveguide structures can achieve high-quality factor (Q-factor) resonance peaks/dips through the generation of guided-mode resonance (GMR), which enables high-resolution biosensing. However, conventional designs face a trade-off between achieving high Q-factor and high sensitivity. To overcome this limitation, this paper proposes a novel reflection-type GMR metasurface that integrates a secondary grating array with a metallic reflector. Unlike traditional transmission-type monolayer grating structures, our design employs a metal reflector positioned between the substrate and the waveguide, which confines the localized electromagnetic fields within the waveguide and prevents their leakage into the substrate. Simultaneously, the secondary grating achieves large spatial overlap between the analyte and the localized electric fields. As a result, under transverse electric (TE)-polarized normal incidence, our metasurface exhibits a pronounced resonance dip with a narrow linewidth, leading to significantly high biochemical sensing sensitivity and high Q-factor. Furthermore, the proposed metasurface demonstrates a measured sensitivity of 420.33 nm/RIU and a specific detection of the biomarker cytokeratin 8 and 18 (CK8/18) associated with gastric cancer cells. Compared with conventional GMR sensor designs, our approach offers not only superior sensing performance but also practical fabrication advantages due to its low aspect ratio grating structure. Therefore, this first proposed reflection-type GMR secondary grating structure provides a promising pathway for the development of high-sensitivity, high Q-factor metasurfaces, with high-precision, high-resolution, high signal-to-noise ratio sensing applications in clinical diagnostics.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"434 ","pages":"Article 137609"},"PeriodicalIF":8.0,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143635645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoactivatable CRISPR/Cas9 lateral flow strip platform for one-pot rapid detection of squamous cell carcinoma antigen DNA in blood

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-15 DOI: 10.1016/j.snb.2025.137607

Zeping Yang , Bin Guo , Chong Hu , Chu Tang , Yingli Shen , Beilei Zhang , Fu Wang

{"title":"Photoactivatable CRISPR/Cas9 lateral flow strip platform for one-pot rapid detection of squamous cell carcinoma antigen DNA in blood","authors":"Zeping Yang , Bin Guo , Chong Hu , Chu Tang , Yingli Shen , Beilei Zhang , Fu Wang","doi":"10.1016/j.snb.2025.137607","DOIUrl":"10.1016/j.snb.2025.137607","url":null,"abstract":"<div><div>Cervical cancer, one of the major threats to women's health, has been gradually decreasing in age of onset and shows a younger trend in recent years. Squamous cell carcinoma antigen (SCCA), as the preferred serum biomarker of cervical cancer, can be effectively screened and detected by quantitative polymerase chain reaction (qPCR). However, the operation process of qPCR is time-consuming and needs to rely on complicated experimental procedures. In this study, we creatively developed a UV light-controlled CRISPR/Cas9 lateral flow strip system for one-pot rapid detection of SCCA DNA in 34 clinical peripheral blood samples. Compared with the commercial SCCA ELISA kit, the photoactivatable detection system not only simplified the whole process without DNA extraction, but also completed the true one-pot rapid detection in 30 minutes, effectively preventing the occurrence of off-target effect and avoiding the risk of sample contamination during the process. In addition, we have successfully solved the incompatibility between recombinase-aided amplification (RAA) and CRISPR system, and increased the minimum detection limit to 10 fg/μL. The detection accuracy has reached an amazing 94.12 %, which is much higher than 79.41 % of the SCCA ELISA kit. Our study indicates that this photoactivatable CRISPR/Cas9 lateral flow strip system has potential application prospects for the early diagnosis of cervical cancer, and further extended to the detection of other related diseases.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"434 ","pages":"Article 137607"},"PeriodicalIF":8.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term stability of electrochemical gas sensors based on capillary forces of electrolyte in porous glass material 基于多孔玻璃材料中电解质毛细力的电化学气体传感器的长期稳定性

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-15 DOI: 10.1016/j.snb.2025.137610

Hyung-Tae Kim , Seung-Wook Kim , Ye-Ji Son , Hyo-Min Kim , Seung-Chul Ha , Dae-Yong Jeong

{"title":"Long-term stability of electrochemical gas sensors based on capillary forces of electrolyte in porous glass material","authors":"Hyung-Tae Kim , Seung-Wook Kim , Ye-Ji Son , Hyo-Min Kim , Seung-Chul Ha , Dae-Yong Jeong","doi":"10.1016/j.snb.2025.137610","DOIUrl":"10.1016/j.snb.2025.137610","url":null,"abstract":"<div><div>Electrochemical gas sensors typically use liquid sulfuric acid electrolytes, prone to evaporation and leakage, degrading performance and limiting lifespan. A porous glass membrane (PGM) was infused with an aqueous sulfuric acid solution to address this. The PGM's porous structure enables capillary action, reducing the exposed surface area of the electrolyte and stabilizing its retention. This capillary-driven mechanism significantly decreases evaporation, enhancing the sensor's long-term stability. In a laminated structure, PGM is an electrolyte reservoir, while a glass microfiber filter (GMF) is a separator and electrolyte supplier. Maintaining equilibrium in electrolyte distribution between these layers ensures consistent gas reaction performance. At 60 °C and 10 ± 5 % RH, the PGM sensor demonstrated a reduced evaporation rate of 3.1 %, compared to 7.1 % for conventional sensors. This study confirms that the capillary effect of PGM effectively minimizes electrolyte evaporation, extends sensor lifespan, and ensures stable sensitivity under extreme conditions.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"434 ","pages":"Article 137610"},"PeriodicalIF":8.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quadruple CHA-assisted assembly of a three-way six-footed DNA walker enabled a waste-free electrochemical and colorimetric dual-mode biosensing

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-15 DOI: 10.1016/j.snb.2025.137605

Xia Yi , Jiahao Wang , Shiyang Zhou, Wang Huang, Guosong Lai

{"title":"Quadruple CHA-assisted assembly of a three-way six-footed DNA walker enabled a waste-free electrochemical and colorimetric dual-mode biosensing","authors":"Xia Yi , Jiahao Wang , Shiyang Zhou, Wang Huang, Guosong Lai","doi":"10.1016/j.snb.2025.137605","DOIUrl":"10.1016/j.snb.2025.137605","url":null,"abstract":"<div><div>DNA walking machines have been used as important signal amplification tools for biosensors; however, how to improve their signal amplification ability and avoid the typical DNA waste problem still needs to be urgently addressed. Herein, we designed a quadruple catalytic hairpin assembly (CHA)-assisted three-way six-footed DNA walker assembly strategy and utilized its signal amplification to develop a novel dual-mode biosensing method. Two CHA cycles were triggered by aptamer recognition reaction, and the other two were triggered by DNA walking-cleavage to a methylene blue (MB)-labeled DNA hairpin and another DNA hairpin immobilized at an electrode. This realized the amplified assembly of the DNA walker and also caused high capture of MB onto electrode to achieve electrochemical signal transduction. In addition, four CHA-triggers were also designed for highly assembling a G-quadruplex-decorated DNA long chain to achieve colorimetric signal transduction. Due to DNA walking-based multiple signal amplification, two wide linear ranges with detection limits down to 1.5 fg mL<sup>–1</sup> and 0.73 pg mL<sup>–1</sup> were endowed to the method for assaying kanamycin antibiotic. The combination of this analytical performance with the simple assay manipulation and full nucleic acid utilization in the method determine its promising value for practical applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"434 ","pages":"Article 137605"},"PeriodicalIF":8.0,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0