Sensors and Actuators B: Chemical最新文献_第5页

Photoelectrochemical biosensor for 5caC-DNA and Exo Ⅲ detection based on Schottky junction integrated with type-I heterojunction of BiOCl/Bi2S3/CS-MXene and hybridization chain reaction

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-19 DOI: 10.1016/j.snb.2025.137647

Huanshun Yin , Yulin Zheng , Chengji Sui , Yunlei Zhou

{"title":"Photoelectrochemical biosensor for 5caC-DNA and Exo Ⅲ detection based on Schottky junction integrated with type-I heterojunction of BiOCl/Bi2S3/CS-MXene and hybridization chain reaction","authors":"Huanshun Yin , Yulin Zheng , Chengji Sui , Yunlei Zhou","doi":"10.1016/j.snb.2025.137647","DOIUrl":"10.1016/j.snb.2025.137647","url":null,"abstract":"<div><div>The 5-carboxylcytosine (5caC), an active DNA demethylation intermediate, is present in many cells and tissues and plays a key role in the regulation of gene expression. 5caC is highly similar in structure to 5-methylcytosine, 5-hydroxymethylcytosine and 5-formylcytosine, but its abundance is extremely low. To explore the biologic function of 5caC, the sensitive detection technique is required. To achieve this goal, a new photoelectrochemical (PEC) biosensor was developed for 5caC-DNA detection using Schottky junction integrated with type-I heterojunction of BiOCl/Bi<sub>2</sub>S<sub>3</sub>/CS-MXene tetany composite as photoactive material and 5caC-hairpin DNA hybridization triggered hybridization chain reaction (HCR) as signal amplification mode. Based on the double heterojunctions, the photoactivity of BiOCl/Bi<sub>2</sub>S<sub>3</sub>/CS-MXene improved greatly, leading to a high photocurrent response, and achieving high detection sensitivity. To perform HCR amplification, an ingenious hairpin DNA containing 5caC was designed to inhibit the cleavage activity of exonuclease III (Exo III). However, after hybridization with <sub>S1</sub>DNA, the unfolded hairpin DNA triggered the excision of double-stranded DNA to release the 5caC-DNA fragment, which further induced the HCR amplification and capture electron donor of methylene blue (MB) onto the electrode surface, causing an increased photocurrent response, and achieving the highly sensitive detection of 5cac-DNA and Exo III. The proposed PEC biosensor showed a detection range of 1 pM - 1 nM and 1–100 U/mL with detection limits of 0.16 pM and 0.34 U/mL (S/N = 3) for 5caC DNA and Exo Ⅲ, respectively. The developed method can also be applied to screen Exo III inhibitor and evaluate the ecotoxic effect of pollutant.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137647"},"PeriodicalIF":8.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143660828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amino nitrogen quantum dots hybrid platinum as efficient peroxidase mimics for fluorescent sensing in microchip

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-18 DOI: 10.1016/j.snb.2025.137641

Jiani Yang, Ling Xia, Gongke Li

{"title":"Amino nitrogen quantum dots hybrid platinum as efficient peroxidase mimics for fluorescent sensing in microchip","authors":"Jiani Yang, Ling Xia, Gongke Li","doi":"10.1016/j.snb.2025.137641","DOIUrl":"10.1016/j.snb.2025.137641","url":null,"abstract":"<div><div>Nanocomposites-based nanozyme have been proposed to realize the combination of the respective properties of each component or to achieve cooperatively enhanced catalytic performances, but the systematic catalytic mechanism remains unclear in majority cases. Herein, an entirely new nanocomposite, amino nitrogen quantum dots hybrid platinum (aNDs/Pt hybrid) nanozyme was fabricated to reach a remarkable peroxidase-like activity owing to the synergetic effects between the two components. The roles of the oxygenous groups on the surface of aNDs/Pt hybrid determining their peroxidase-like catalytic activity were evaluated by regulating the functional groups during the synthesis of nitrogen quantum dots. Accordingly, empirical and theoretical evidences were given to identify the catalytic, substrate-binding, and inhibition sites in peroxidase-mimicking catalytic reactions of the aNDs/Pt hybrid. Meanwhile, the catalytic activity of aNDs/Pt hybrid nanozyme shows a strong dependence on the concentration of H<sub>2</sub>O<sub>2</sub>, based on which a highly sensitive fluorescent sensor for glucose detection in a microchip was established by employing aNDs/Pt hybrid as robust peroxidase. It is found that the developed sensor had excellent glucose sensing capability, including two-section broad linear detection ranges (0.1–6.0 μmol/L and 6.0–1000.0 μmol/L), less sample consumption (2.0 μL) and a low detection limit (31.7 nmol/L). Human serum and saliva samples were successfully examined by the as-proposed sensor with satisfactory results, showing the practical applications. This work not only gives a much deeper understanding on cooperatively catalytic mechanism for nanohybrids-based nanozymes, but also facilitate the design and fabrication of other types of target-specific artificial enzymes.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137641"},"PeriodicalIF":8.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated microfluidic device of DNA extraction, recombinase polymerase amplification and micro-capillary electrophoresis for sample-to-answer detection of Salmonella Typhimurium

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-18 DOI: 10.1016/j.snb.2025.137625

Van Dan Nguyen, Gayathri Sureshkumar, Tae Seok Seo

{"title":"Integrated microfluidic device of DNA extraction, recombinase polymerase amplification and micro-capillary electrophoresis for sample-to-answer detection of Salmonella Typhimurium","authors":"Van Dan Nguyen, Gayathri Sureshkumar, Tae Seok Seo","doi":"10.1016/j.snb.2025.137625","DOIUrl":"10.1016/j.snb.2025.137625","url":null,"abstract":"<div><div>Foodborne pathogens pose a significant global health threat, leading to millions of illnesses and deaths annually. Our study introduces an integrated microfluidic chip for rapid molecular diagnostics of foodborne pathogens. The proposed device can perform DNA extraction, recombinase polymerase amplification (RPA), and micro-capillary electrophoresis (µCE) in a sequential manner. With a compact copper-based thermal unit for RPA and precise on-off power supply for the µCE, we could achieve swift detection <em>InvA</em> gene of <em>Salmonella</em> Typhimurium within 75 min. The entire process includes 10 min for sample lysis, 10 min for genomic DNA extraction, 50 min for the RPA reaction, and 5 min for µCE operation and peak assignment. Within 5 min, we were able to detect the presence of the <em>InvA</em> gene of <em>S.</em> Typhimurium (161 bp) with a LOD of 10<sup>3</sup> CFU/mL in both bacterial culture and spiked milk samples. We used a short bracket ladder (121 bp) and a long bracket ladder (203 bp) to accurately assign the target peak in the electropherogram, and the relative migration time of target gene over two bracket ladders was 0.449 ± 0.0094. Notably, our platform is compact, with all components neatly arranged within a 3D printed frame measuring 12 cm (width) × 14 cm (length) × 2.7 cm (height), excluding the in-house detector. This integrated microsystem from the sample injection to the CE analysis presents promising prospects for advanced on-site pathogen detection, and lays the groundwork for future molecular diagnostic platform to combat foodborne illnesses and understanding of pathogen genome and variations.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137625"},"PeriodicalIF":8.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial intelligence-based gastric cancer detection in the gastric submucosal dissection method via hyperspectral imaging

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-18 DOI: 10.1016/j.snb.2025.137630

Inyoung Park , Jin Roh , Dohyeon Son , Choong-Kyun Noh , Jonghee Yoon

{"title":"Artificial intelligence-based gastric cancer detection in the gastric submucosal dissection method via hyperspectral imaging","authors":"Inyoung Park , Jin Roh , Dohyeon Son , Choong-Kyun Noh , Jonghee Yoon","doi":"10.1016/j.snb.2025.137630","DOIUrl":"10.1016/j.snb.2025.137630","url":null,"abstract":"<div><div>Although endoscopy is a standard diagnostic tool for detecting gastric cancer, challenges persist in identifying cancer and assessing tumor extent, particularly in stomachs with atrophy and intestinal metaplasia. To address this issue, we aimed to introduce a novel, compact hyperspectral imaging system with artificial intelligence (AI) that utilizes structured illumination and hyperspectral imaging to diagnose gastric cancer based on intrinsic tissue optical properties. The optical properties of three types of gastric tissue (normal, adenoma, and gastric cancer) obtained from nine patients collected via endoscopic submucosal dissection were analyzed. Our findings reveal that cancer tissue displays unique optical properties, such as low reduced scattering coefficients and distinct reflectance spectral profiles when compared to normal and adenoma tissues. However, it was challenging to diagnose gastric cancer accurately using optical properties with conventional analysis methods due to their heterogeneity. Therefore, we employed a Vision Transformer model with a supervised learning approach to accurately classify tissue types based on intrinsic optical properties. To accurately train the AI model, we devised a novel image processing method to obtain single-pixel level ground-truth labeling data by aligning pathology results and imaging data. The trained AI model successfully demonstrated its ability to diagnose gastric cancer accurately in the nine patients studied. Given its compact design and rapid imaging capabilities, the proposed optical system can be a versatile clinical tool for on-site endoscopic diagnosis and could potentially aid in complete endoscopic tumor removal.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137630"},"PeriodicalIF":8.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wireless powered flexible carbon dioxide gas monitoring device utilizing polyethyleneimine functionalized GO/MXene composites for nondestructive dynamic monitoring of mango ripeness

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-18 DOI: 10.1016/j.snb.2025.137633

Laizhao Guo , Wentao Huang , Yun Li , Xiaoshuan Zhang , Xiang Wang

{"title":"Wireless powered flexible carbon dioxide gas monitoring device utilizing polyethyleneimine functionalized GO/MXene composites for nondestructive dynamic monitoring of mango ripeness","authors":"Laizhao Guo , Wentao Huang , Yun Li , Xiaoshuan Zhang , Xiang Wang","doi":"10.1016/j.snb.2025.137633","DOIUrl":"10.1016/j.snb.2025.137633","url":null,"abstract":"<div><div>Mangoes are usually packed in cardboard boxes during transportation and storage, and manual unpacking of the boxes to determine their ripeness is an inefficient method of testing. Fortunately, changes in carbon dioxide (CO₂) concentration can serve as an indicator of ripeness and potential spoilage. To address this, a wireless powered flexible CO₂ gas monitoring device (WFCMD) utilizing polyethyleneimine (PEI)-functionalized GO/MXene composites was developed. This device demonstrates high sensitivity and excellent electrical conductivity, making it suitable for real-time monitoring in practical applications. The response of the composites to CO₂ was evaluated with varying PEI loadings, showing enhanced sensing performance due to the efficient CO₂ adsorption by PEI and the high conductivity of GO/MXene. The complete gas monitoring system was integrated using an ESP-12F microcontroller unit (MCU), allowing for seamless attachment to the surface of mangoes for continuous, nondestructive monitoring. The device's feasibility was confirmed through extended monitoring of packaged mangoes, demonstrating its potential for application in fruit gas detection and quality control.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137633"},"PeriodicalIF":8.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous dielectrophoretic sorting of liquid beads

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-18 DOI: 10.1016/j.snb.2025.137627

Ajeet Singh Yadav, Aditya Vashi, Uditha Roshan, Fariba Malekpour Galogahi, Du Tuan Tran, Kamalalayam Rajan Sreejith, Nam-Trung Nguyen

{"title":"Continuous dielectrophoretic sorting of liquid beads","authors":"Ajeet Singh Yadav, Aditya Vashi, Uditha Roshan, Fariba Malekpour Galogahi, Du Tuan Tran, Kamalalayam Rajan Sreejith, Nam-Trung Nguyen","doi":"10.1016/j.snb.2025.137627","DOIUrl":"10.1016/j.snb.2025.137627","url":null,"abstract":"<div><div>We report the concept of continuous sorting of liquid beads using dielectrophoresis (DEP) in a microfluidic device. Liquid beads are liquid droplets encapsulated in a hard polymer shell, which provides a unique core-shell structure crucial for applications such as digital polymerase chain reaction (dPCR), digital loop-mediated isothermal amplification (LAMP), drug delivery, cell cultures and microreactors. A population of consistent microscale liquid beads are required for these applications. We utilised microfluidic methods to continuously produce beads with a narrow size distribution. However, this technique requires a precise control of flow rates to prevent the formation of core-less beads. We generated monodispersed liquid beads with trimethylolpropane trimethacrylate (TMPTM) shell and hydrofluoroether (HEF) core. The device produced about 20–30 % core-less solid beads, which needs to be removed from the population of liquid beads. We first examined the dielectrophoretic (DEP) responses for liquid beads and solid beads. Using a relatively simple setup with a steel needle and indium tin oxide (ITO)-coated glass electrodes, we demonstrated that beads exhibit both positive and negative DEP under alternate-current (AC) and direct-current (DC) electric fields. Subsequently, we designed and tested a microfluidic sorting device to leverage the differential DEP responses for continuous sorting of liquid beads and solid beads. In a DC field, solid beads experience positive DEP, whereas liquid beads show negative DEP, enabling their separation with an efficiency of approximately 80 %. Our sorting method provides a precise, controllable, label-free, and scalable solution for obtaining consistent liquid beads for further applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137627"},"PeriodicalIF":8.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laser-scribed flexible interdigitated electrodes using DIY laser engraver and electrodeposition of polyaniline and silver for pH monitoring

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-18 DOI: 10.1016/j.snb.2025.137634

Krishnaveni K , Adityan S , Murugasenapathi N K , Tamilarasan Palanisamy

{"title":"Laser-scribed flexible interdigitated electrodes using DIY laser engraver and electrodeposition of polyaniline and silver for pH monitoring","authors":"Krishnaveni K , Adityan S , Murugasenapathi N K , Tamilarasan Palanisamy","doi":"10.1016/j.snb.2025.137634","DOIUrl":"10.1016/j.snb.2025.137634","url":null,"abstract":"<div><div>It is crucial to have an impartial indicator to assess the progress of healing, considering the complexity of the wound healing process. Here, sensitivity, repeatability and accuracy are the optimal criteria for such a measurement along with the elimination of patient discomfort. In this work, a flexible pH sensor has been developed with polyaniline and silver electrodeposited on laser-scribed interdigitated electrodes. Importantly, the fabrication of laser-induced graphene (LIG) based interdigitated electrodes using a low-cost DIY (do-it-yourself) diode laser engraver has been demonstrated with a critical strategy to improve conductivity. Subsequently, polyaniline (PANI) was electropolymerized on LIG through voltammetric cycles over a potential range from −0.1–1.2 V at 100.0 mV/s scan rate. The Ag/AgCl pseudo-reference electrode was also electrodeposited on the interdigitated LIG pattern under galvanostatic conditions with subsequent electrochemical chlorination. The obtained sensors showed an average responsivity of 47.9 mV/pH, which is comparable to the Nernstian value (59.0 mV/pH) in the 4.0–8.0 pH range. The response time, cyclical, and temporal stability of the sensor were tested, which showed a rapid and stable response. Overall, the developed sensor accurately monitors pH values in the physiologically significant range for wound healing with the added advantage of low capital expenditure, making it a cost-effective option.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"434 ","pages":"Article 137634"},"PeriodicalIF":8.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ NO sensing in methane/ammonia premixed flame by calibration-free Faraday Rotation Spectroscopy

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-18 DOI: 10.1016/j.snb.2025.137628

Ze Xu, Ning Zhu, Xing Chao

{"title":"In situ NO sensing in methane/ammonia premixed flame by calibration-free Faraday Rotation Spectroscopy","authors":"Ze Xu, Ning Zhu, Xing Chao","doi":"10.1016/j.snb.2025.137628","DOIUrl":"10.1016/j.snb.2025.137628","url":null,"abstract":"<div><div>With the rapid development of ammonia combustion, the detection of NO<sub>x</sub> content in flame has become an increasingly important task. While infrared tunable diode laser absorption spectroscopy (TDLAS) has presented a viable solution to combustion diagnostics for temperature and species detection, difficulties such as transmission baseline fitting and spectral interference still remain to be major hurdles for its deployment in complex practical environment. A novel <em>in situ</em> NO sensing system based on Faraday Rotation Spectroscopy (FRS) is developed and deployed for calibration-free ammonia flame measurement in this work. Laser absorption from diamagnetic species including H<sub>2</sub>O and CO<sub>2</sub> in flame bears no influence from the exerted magnetic field, such that NO as a paramagnetic species can be detected with high sensitivity. A group of R-branch transition lines from the mid-infrared fundamental band of NO near 1884.3 cm<sup>−1</sup> are selected for NO concentration detection, and a new strategy is developed for simultaneous temperature inference so that no on-site calibration will be needed. Flame measurement results are validated against thermocouple and conventional direct absorption measurements, and temperature and concentration uncertainty of 3.0 % and 4.1 % has been evaluated from experimental data analysis. NO detection limit of 5.1 ppm-m is achieved over 1000–2000 K in ammonia-seeded methane flames. The study shows great potential of tunable diode laser FRS sensors for <em>in situ</em>, calibration-free, and quantitative species diagnostics in combustion flames, where accuracy and sensitivity are typically hindered by the harsh, dynamically changing practical conditions.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137628"},"PeriodicalIF":8.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel red-emitting fluorescent probe reveals the depletion of vicinal dithiol-containing proteins in acute alcoholic liver injury (AALI) mice model

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-18 DOI: 10.1016/j.snb.2025.137639

Zahid Nasim Sayed , Ruipeng Shen , Jirong Liu , Qiyu Chen , Haijuan Zhang , Jingyi Liu , Alsiddig Osama , Shah Fahad , Baoxin Zhang , Jianguo Fang

{"title":"A novel red-emitting fluorescent probe reveals the depletion of vicinal dithiol-containing proteins in acute alcoholic liver injury (AALI) mice model","authors":"Zahid Nasim Sayed , Ruipeng Shen , Jirong Liu , Qiyu Chen , Haijuan Zhang , Jingyi Liu , Alsiddig Osama , Shah Fahad , Baoxin Zhang , Jianguo Fang","doi":"10.1016/j.snb.2025.137639","DOIUrl":"10.1016/j.snb.2025.137639","url":null,"abstract":"<div><div>Vicinal dithiol-containing proteins (VDPs) are a special type of redox-active thiol-carrying ubiquitous proteins with essential role in maintaining redox balance. The abnormal cellular levels of VDPs are known to be implicated in numerous pathological conditions including cancer, diabetes, stroke and neurodegenerative diseases. Therefore, developing small molecule fluorescent tools for the sensitive and selective detection of VDPs is indispensable. Herein, we rationally designed and screened a red-emitting fluorescent probe (<strong>FRP-6</strong>) carrying α, β-unsaturated acrylamide as a receptor unit, for the precise detection of VDPs. The probe demonstrated excellent capability to identify its target both in the living cells and <em>in vivo</em> without being interfered by other biologically relevant analytes. Additionally, <strong>FRP-6</strong> was employed to detect the depletion of VDPs levels in drug induced liver injury (DILI) and acute alcoholic liver injury (AALI) models.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137639"},"PeriodicalIF":8.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust nanozyme with dense crystalline-amorphous interfaces for efficient detection of reactive oxygen species in saliva

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-03-17 DOI: 10.1016/j.snb.2025.137571

Zhiqiang Zhai , Rui Liao , Hao Wei , Heng Zheng , Yin Long , Huiling Tai , Xiaosong Du , Yadong Jiang

{"title":"Robust nanozyme with dense crystalline-amorphous interfaces for efficient detection of reactive oxygen species in saliva","authors":"Zhiqiang Zhai , Rui Liao , Hao Wei , Heng Zheng , Yin Long , Huiling Tai , Xiaosong Du , Yadong Jiang","doi":"10.1016/j.snb.2025.137571","DOIUrl":"10.1016/j.snb.2025.137571","url":null,"abstract":"<div><div>Real-time monitoring of reactive oxygen species (ROS) concentrations in biological fluids using enzyme-catalyzed reactions to generate electrical signals holds promise for early diagnosis of various ROS-related diseases. However, challenges such as small sample volumes, low concentrations and the short lifespan of ROS hinders its translational application. Here, a phase engineered nanozyme with highly oriented dense crystalline-to-amorphous interfaces were synthesized and integrated into flexible sensors for trace-level ROS detections, achieving a limit of detection (LOD) of 42 nM (1.428 ppb) with only 13.5 μL sample volume and a high sensitivity of 1.971 mA·mM<sup>−1</sup>cm<sup>−2</sup>, surpassing traditional benchmarks. The outstanding enzyme-like activity of our engineered nanozyme is attributed to the highly oriented dense crystalline-to-amorphous interface. Systematic structure-dependent tests and multi-characterization analyses revealed that these dense crystalline-to-amorphous interfaces can effectively increase the density of the oxygen vacancy defects, crucial intermediates in the multiphasic redox reactions. Furthermore, <em>in situ</em> Raman spectroscopy indicated that the dense crystalline-to-amorphous interface promotes the phase transition from oxides to highly reactive hydroxyl oxides and stabilize the active phase throughout the Fenton reaction. The integration of carbon nanotubes (CNTs) with high conductivity and efficient charge transport properties significantly enhances the sensor performance. Real-time and accurate sensor responses to short-lived ROS, further validated by in-situ Fenton reactions and ESR analysis of inflamed patient saliva, highlights potential for long-term oral inflammation monitoring.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137571"},"PeriodicalIF":8.0,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0