Sensors and Actuators B: Chemical最新文献

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Hydrogel-polymer hybrid actuator with soft lattice skeleton for excellent connectivity
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2025-02-01 DOI: 10.1016/j.snb.2025.137377
Haruna Kozuki , Koki Yoshida , Hiroki Yasuga , Yuta Kurashina
{"title":"Hydrogel-polymer hybrid actuator with soft lattice skeleton for excellent connectivity","authors":"Haruna Kozuki ,&nbsp;Koki Yoshida ,&nbsp;Hiroki Yasuga ,&nbsp;Yuta Kurashina","doi":"10.1016/j.snb.2025.137377","DOIUrl":"10.1016/j.snb.2025.137377","url":null,"abstract":"<div><div>Soft actuators, which integrate stimuli-responsive hydrogels with flexible polymers, have been extensively studied to develop <em>in vivo</em> soft robots for drug delivery systems and non-invasive treatments. However, conventional bilayer actuators, consisting of hydrogel and polymer layers, pose a challenge: the necessity for chemical modification to connect the two layers imposes limitations on the selection of usable materials. This study presents a hydrogel-polymer hybrid actuator comprising a stimuli-responsive hydrogel layer and a hydrogel-polymer hybrid layer with a three-dimensional (3D) porous polymer “soft lattice skeleton.” The soft lattice skeleton improves connectivity between layers through a mechanical interlock, eliminating the need for chemical modification. A temperature-responsive poly(<em>N</em>-isopropylacrylamide) hydrogel was used as the driving source of the actuator. The soft lattice skeleton was fabricated using multi-directional photolithography. Connectivity improvement was demonstrated by comparing the hybrid actuator to a conventional bilayer actuator after thermal deformation cycles. Specifically, the hybrid actuator maintained connectivity even after multiple cycles, while the bilayer actuator showed complete exfoliation. Deformation behavior was controlled by adjusting the design of the soft lattice skeleton, specifically the width of its constituent micropillars. Curved actuators with various pillar widths exhibited distinct deformation patterns, including bending in the same or opposite direction as the preset curve or twisting. This hybrid actuator design offers improved durability and programmable deformation without chemical modification. It will enable the selection of various hydrogel materials for implementing diverse functions, including pH and glucose sensing. Future research should explore additional structural parameters of the soft lattice skeleton to further control deformation behavior.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137377"},"PeriodicalIF":8.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a compact and efficient FAIMS ionization source based on UV-LED photoelectric effect
IF 8.4 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2025-02-01 DOI: 10.1016/j.snb.2025.137379
Mengchao Jin, Shaomin Liu, Han Wang, Youjiang Liu, Jianhui Ji, Jie Sheng, Shenglai Zhen, Chilai Chen
{"title":"Development of a compact and efficient FAIMS ionization source based on UV-LED photoelectric effect","authors":"Mengchao Jin, Shaomin Liu, Han Wang, Youjiang Liu, Jianhui Ji, Jie Sheng, Shenglai Zhen, Chilai Chen","doi":"10.1016/j.snb.2025.137379","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137379","url":null,"abstract":"A compact photoelectric effect ionization source for high-field asymmetric waveform ion mobility spectrometry (FAIMS) based on UV-LED was proposed in this paper. Ambient air and three electronegative compounds (SF<sub>6</sub>, NO<sub>2</sub>, and SO<sub>2</sub>) were selected as samples to verify the effectiveness of the proposed method, and the effects of the emission electrode material, light source, relative humidity, repeller voltage, and flow rate of carrier gas were investigated. Distinctive fingerprint spectra were successfully obtained for the air background and the three compounds, exhibiting the method's discrimination capability. The silver (Ag) was identified as the optimal material for the emission electrodes, capable of generating an ion signal intensity of -77.8 pA in an air background. The repeller voltage and flow rate have a significant impact on ionization efficiency. An increased flow rate was associated with enhanced ionization efficiency, necessitating a corresponding increase in the repeller voltage. When the flow rate of carrier gas is 5<!-- --> <!-- -->L/min, the optimal repeller voltages for the three samples (SF<sub>6</sub>, NO<sub>2</sub>, and SO<sub>2</sub>) were -2 V, -0.5<!-- --> <!-- -->V, and -1 V, respectively, which roughly corresponded to their ion mobility. With increasing concentration of the samples, the ion signal intensity linearly increases at first and then levels off. Different samples show significant differences in the growth rates in the linear region, and the detection limits for the three substances, SF<sub>6</sub>, NO<sub>2</sub>, and SO<sub>2</sub>, were 0.028 ppm, 0.014 ppm, and 0.033 ppm, respectively.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"13 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a miniaturized current type H2 gas sensor based on Nafion and Pt-Pd/C sensing electrode
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2025-02-01 DOI: 10.1016/j.snb.2025.137366
Hongcan Zhang, Lingchu Huang, Yuanlin Li, Yongshun Liu, Xishuang Liang, Geyu Lu
{"title":"Development of a miniaturized current type H2 gas sensor based on Nafion and Pt-Pd/C sensing electrode","authors":"Hongcan Zhang,&nbsp;Lingchu Huang,&nbsp;Yuanlin Li,&nbsp;Yongshun Liu,&nbsp;Xishuang Liang,&nbsp;Geyu Lu","doi":"10.1016/j.snb.2025.137366","DOIUrl":"10.1016/j.snb.2025.137366","url":null,"abstract":"<div><div>In this manuscript, a miniaturized current type gas sensor based on the membrane electrode assemble (MEA) composed of Nafion and Pt-Pd/C sensing electrode has been developed to realize the effective detection of H<sub>2</sub> at room temperature. The newly designed sensor is significantly smaller than current common gas sensors, and the structure of the sensor and test fixture has been redesigned. The miniature sensor is easy to carry, install and apply to other devices on a daily basis, the manufacturing cost is greatly reduced, and precious metals can be used in the selection of gas-sensitive materials. In the experiment, we used Pt and Pd bimetallic stretcher loaded on carbon as sensing material, both noble metals are sensitive to H<sub>2</sub> and have high catalytic activity, which enables efficient detection of H<sub>2</sub> gas. The sensor could detect H<sub>2</sub> at levels as low as 3 ppm with a −0.163 nA response and had a good linear relationship in range of 30–6000 ppm H<sub>2</sub>. In addition, this amperometric H<sub>2</sub> sensor displayed fast response-recovery rate as well as excellent selectivity and stability. Within the explosive limits of H<sub>2</sub>, our sensors enable fast and accurate detection. Such a miniaturized device has a good application prospect due to its advantages.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137366"},"PeriodicalIF":8.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harnessing a multifunctional far-red fluorescence platform for high-performance biological sensing and imaging
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2025-01-31 DOI: 10.1016/j.snb.2025.137370
Zi Yi Xu , Jia Shan Xia , Rong Wang , Qi Xiao , Hong Qun Luo , Tao Deng , Nian Bing Li
{"title":"Harnessing a multifunctional far-red fluorescence platform for high-performance biological sensing and imaging","authors":"Zi Yi Xu ,&nbsp;Jia Shan Xia ,&nbsp;Rong Wang ,&nbsp;Qi Xiao ,&nbsp;Hong Qun Luo ,&nbsp;Tao Deng ,&nbsp;Nian Bing Li","doi":"10.1016/j.snb.2025.137370","DOIUrl":"10.1016/j.snb.2025.137370","url":null,"abstract":"<div><div>Fluorescence imaging has provided abundant information in biological researches, which unveils the microscopic nexuses in physiological and pathological processes. Nevertheless, this main-stream solution is always impeded by intractable drawbacks of imaging tools including small Stokes shift, short-wavelength emission, inferior photostability, and insufficient fluorescence quantum yield (<em>Φ</em><sub>F</sub>). In this contribution, an ingenious molecular platform constituted of three novel dicyanocoumarin analogues is rationally engineered. With large Stokes shift, its fluorescence is located in the far-red region. Serendipitously, an unexpected phenomenon is uncovered that emission of these novel compounds enhances along with the increase of polarity, which confers them considerable <em>Φ</em><sub>F</sub>. Due to such merits, this multifunctional fluorescence platform is applied to cellular imaging due to its multivariate spectral responsiveness toward pH, polarity, and hypochlorous acid (HClO). Besides, in vivo imaging of ferroptosis and wound inflammation mouse models has also revealed the great potential of this fluorescence platform. Performing eminent bioimaging results, we conceive that this fluorescence platform would be a robust imaging toolbox in the future, which provides designing inspiration for development of high-performance fluorophores.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137370"},"PeriodicalIF":8.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Real-time nitric oxide and inflammation sensing in 2D osteoarthritis models: microsensor design and application
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2025-01-31 DOI: 10.1016/j.snb.2024.137147
Laura Belcastro , Paolo Antonacci , Gaia Rocchitta , Christoph M. Sprecher , Andrea J. Vernengo , Pier Andrea Serra , Fabiana Arduini , Sibylle Grad , Valentina Basoli
{"title":"Real-time nitric oxide and inflammation sensing in 2D osteoarthritis models: microsensor design and application","authors":"Laura Belcastro ,&nbsp;Paolo Antonacci ,&nbsp;Gaia Rocchitta ,&nbsp;Christoph M. Sprecher ,&nbsp;Andrea J. Vernengo ,&nbsp;Pier Andrea Serra ,&nbsp;Fabiana Arduini ,&nbsp;Sibylle Grad ,&nbsp;Valentina Basoli","doi":"10.1016/j.snb.2024.137147","DOIUrl":"10.1016/j.snb.2024.137147","url":null,"abstract":"<div><div>Nitric oxide (NO), a key metabolite released during inflammation, can be a reliable biomarker for conditions such as trauma, infection or during inflammation. Standard NO detection methods, including electron paramagnetic resonance (EPR) and indirect detection of nitrites as NO derivatives, using Griess assay, are limited in their ability to provide real-time monitoring, a critical feature for studying drug responses and enabling clinical applications. Electrochemical sensors can be used for continuous real-time NO measurement but require careful design to ensure selectivity in complex biological environments. In this study, platinum wire electrodes were used, either unmodified or coated with poly-o-phenylenediamine (PPD) polymer, to improve selectivity. These sensors were calibrated using S-nitroso-N-acetylpenicillamine (SNAP) molecule, an NO-donor, and tested against common biological interferents, such as ascorbic acid, fetal bovine serum, antibiotics, and several electrolytes. For real time NO detection, a tissue culture plate was customized to allow sensor connection guaranteeing a closed and sterile environment for cell culture. Amperometry was performed over 48 h in 2D chondrocyte inflammation model cultures with and without IL-1β stimulation. Results demonstrated that the PPD coating was homogeneously distributed on the sensor surface and significantly reduced background noise from ascorbic acid. Furthermore, PPD-modified sensors showed enhanced selectivity and reproducibility during real-time NO monitoring, particularly in distinguishing NO levels in inflamed versus non-inflamed chondrocyte cultures. The findings highlight the importance of surface modifications to mitigate interference from non-relevant analytes in complex biological environments. These results suggest that real-time NO monitoring with electrochemical sensors could offer a valuable tool for assessing inflammation, with potential future applications in both in vitro and in vivo settings.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137147"},"PeriodicalIF":8.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioorthogonal Raman and IR probes for live cell metabolomics: A library
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2025-01-30 DOI: 10.1016/j.snb.2025.137363
Wiktoria Korona , Barbara Orzechowska , Kacper Siąkała , Anna Maria Nowakowska , Anna Pieczara , Szymon Buda , Robert Pawlowski , Jacek Mlynarski , Malgorzata Baranska
{"title":"Bioorthogonal Raman and IR probes for live cell metabolomics: A library","authors":"Wiktoria Korona ,&nbsp;Barbara Orzechowska ,&nbsp;Kacper Siąkała ,&nbsp;Anna Maria Nowakowska ,&nbsp;Anna Pieczara ,&nbsp;Szymon Buda ,&nbsp;Robert Pawlowski ,&nbsp;Jacek Mlynarski ,&nbsp;Malgorzata Baranska","doi":"10.1016/j.snb.2025.137363","DOIUrl":"10.1016/j.snb.2025.137363","url":null,"abstract":"<div><div>Vibrational techniques using bioorthogonal Raman and infrared (IR) probes that emit unique spectral signals in the silent region (2800–1800 cm⁻¹) have gained considerable attention across various fields of biomedicine. Labeled Raman and infrared microscopies are proving to be highly beneficial in studying the dynamics of metabolic processes within single living cells. Disruption or deregulation of these systems leads to a range of diseases and makes understanding the biochemistry of metabolism one of the greatest challenges in biology. In this study, we built a library of Raman and IR probes to assess metabolic changes, categorizing them into four groups related to glucose and lipid metabolism, as well as the activity of the cell nucleus and mitochondria. We highlight the effectiveness of vibrational labeling in three key areas: tracking specific metabolic pathways, multiplex imaging, and multimodal imaging. Single-pathway analysis was achieved using selected molecular probes (e.g., azido-palmitic acid, EdU, d<sub>7</sub>-glucose, MitoBADY), each targeting different metabolic pathways. Multiplex imaging allowed for the simultaneous tracking of lipid and mitochondrial metabolism, providing a more comprehensive view of the cell's biomolecular state. Furthermore, a multimodal approach, which combined Raman and optical photothermal infrared (O-PTIR) techniques, enabled the complementary characterization of endothelial cells labeled with azide- and deuterium-palmitic acid. Both high-resolution imaging techniques and microscopy facilitated subcellular analysis in living and fixed cells, also in aqueous environments. These findings underscore the value of integrating vibrational labeling with advanced cell imaging to expand the horizons of metabolomics research.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137363"},"PeriodicalIF":8.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effective detection of kanamycin residues by target recycling-based enzyme-free signal dual amplification electrochemical aptasensor
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2025-01-30 DOI: 10.1016/j.snb.2025.137365
Guochao Chen , Lingzhi Wang , Huan Liu , Lei Wang , Changyuan Yu , Hao Li
{"title":"Effective detection of kanamycin residues by target recycling-based enzyme-free signal dual amplification electrochemical aptasensor","authors":"Guochao Chen ,&nbsp;Lingzhi Wang ,&nbsp;Huan Liu ,&nbsp;Lei Wang ,&nbsp;Changyuan Yu ,&nbsp;Hao Li","doi":"10.1016/j.snb.2025.137365","DOIUrl":"10.1016/j.snb.2025.137365","url":null,"abstract":"<div><div>Abuse of kanamycin is still present in livestock and poultry farming, thereby causing serious damage to the human body through food chain. Therefore, it is necessary to effectively detect kanamycin in animal sourced food. Here, four different versions of electrochemical aptasensors were developed for efficient detection of kanamycin; especially, a target recycling-based enzyme-free signal dual amplification electrochemical aptasensor (<em>i.e.</em>, named as Ver. 4 aptasensor) targeting kanamycin was developed. Under the optimized conditions, the Ver. 4 aptasensor exhibited a high sensitivity toward kanamycin with a limit of detection (LOD) of 0.10 ng/mL (linear range from 0.48 to 4844.99 ng/mL), and a high selectivity against the kanamycin’s structural analogs. Meanwhile, good reusability and excellent detection repeatability further indicated the application feasibility of the Ver. 4 aptasensor. Moreover, the recovery rate of kanamycin from spiked milk samples ranged from 93.24 % to 103.80 %. Results presented here suggested the application potential of the Ver. 4 aptasensor for effectively detecting kanamycin in animal sourced foods.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137365"},"PeriodicalIF":8.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ppb-level photoacoustic sensor system for SF6 decomposition component HF using fuzzy c-means algorithm based on baseline correction
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2025-01-30 DOI: 10.1016/j.snb.2025.137319
Mu Liang , Xuechao Du , Mengya Lu, Shen Tian, Yingying Qiao, Yang Gao, Pengbo Chen, Mingqi Jiao, Lei Li, Chongxin Shan
{"title":"Ppb-level photoacoustic sensor system for SF6 decomposition component HF using fuzzy c-means algorithm based on baseline correction","authors":"Mu Liang ,&nbsp;Xuechao Du ,&nbsp;Mengya Lu,&nbsp;Shen Tian,&nbsp;Yingying Qiao,&nbsp;Yang Gao,&nbsp;Pengbo Chen,&nbsp;Mingqi Jiao,&nbsp;Lei Li,&nbsp;Chongxin Shan","doi":"10.1016/j.snb.2025.137319","DOIUrl":"10.1016/j.snb.2025.137319","url":null,"abstract":"<div><div>In this paper, we present for the first time a photoacoustic sensor that uses a fuzzy c-means algorithm based on baseline correction to detect HF gas in the presence of an SF<span><math><msub><mrow></mrow><mrow><mn>6</mn></mrow></msub></math></span> background. This algorithm was proposed to address the issue of unstable amplitude of the second harmonic signal detected by photoacoustic sensors in the near-infrared region due to the weak absorption intensity of gases. The fuzzy c-means algorithm based on baseline correction mainly consists of two parts: firstly, to address the issue of baseline drift in the second harmonic signal, an adaptive iterative reweighted penalty least squares (airPLS) algorithm is employed to remove the drifting baseline, thus stabilizing the signal; secondly, instead of solely relying on the peak value of second harmonic signal for gas concentration calibration, multiple characteristic values of the signal are extracted to form a vector, a fuzzy c-means (FCM) algorithm is then utilized for clustering analysis in a three-dimensional space, using cluster centroids to calibrate gas concentration. The experimental results demonstrate that following algorithmic processing, the R<span><math><msup><mrow></mrow><mrow><mn>2</mn></mrow></msup></math></span> value of the calibration line increases from 0.984 to 0.997, and the minimum detection limit at 1<span><math><mi>σ</mi></math></span> noise level for HF decreases from 295 ppb to 88 ppb.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137319"},"PeriodicalIF":8.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143056389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced detection of abrin: Unraveling AP lyase activity and pioneering innovative assays for advanced biosecurity applications
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2025-01-30 DOI: 10.1016/j.snb.2025.137362
Tingting Liu , Lina Dong , Jing Lv , Bin Ji , Susu Xia , Jiaxin Li , Jing Wang , Jinglin Wang , Jian Zhang , Lihua Tan , Han Jin , Shan Gao , Lin Kang , Wenwen Xin
{"title":"Enhanced detection of abrin: Unraveling AP lyase activity and pioneering innovative assays for advanced biosecurity applications","authors":"Tingting Liu ,&nbsp;Lina Dong ,&nbsp;Jing Lv ,&nbsp;Bin Ji ,&nbsp;Susu Xia ,&nbsp;Jiaxin Li ,&nbsp;Jing Wang ,&nbsp;Jinglin Wang ,&nbsp;Jian Zhang ,&nbsp;Lihua Tan ,&nbsp;Han Jin ,&nbsp;Shan Gao ,&nbsp;Lin Kang ,&nbsp;Wenwen Xin","doi":"10.1016/j.snb.2025.137362","DOIUrl":"10.1016/j.snb.2025.137362","url":null,"abstract":"<div><div>Abrin, a potent ribosome-inactivating protein, is a serious threat as a biological warfare agent. Developing rapid and accurate assays for detecting abrin are crucial for early prevention of related attacks. In this study, we systematically demonstrate that abrin exhibits apurinic/apyrimidinic (AP) lyase activity, with optimal enzymatic function at 63 °C and pH 4.4. Based on this discovery, we create a fluorescence signaling assay (FSA) for swift detecting abrin and further integrate it with a lateral flow assay (LFA), resulting in a novel, portable detection platform (FSA-LFA). This combined assay allows for sensitive detection of abrin at the picogram level, surpassing the limitations of traditional <em>N</em>-glycosidase-based assays. The FSA-LFA platform is cost-effective, highly sensitive, and amenable to field use. Furthermore, it can be readily adapted for the detection of other ribosome-inactivating proteins, offering broad potential for rapid identification of biosecurity threats.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137362"},"PeriodicalIF":8.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143056678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Disposable microfluidic chip embedded core/shell nylon6/protein corona immunofiber mats for analysis of circulating tumor cell attachment behavior in preclinical trials
IF 8 1区 化学
Sensors and Actuators B: Chemical Pub Date : 2025-01-30 DOI: 10.1016/j.snb.2025.137364
I‑Ting Peng , Chih-Chia Cheng , Hui-Ling Hsu , Feng-Ping Lin , Chien-Hsing Lu , Jem-Kun Chen
{"title":"Disposable microfluidic chip embedded core/shell nylon6/protein corona immunofiber mats for analysis of circulating tumor cell attachment behavior in preclinical trials","authors":"I‑Ting Peng ,&nbsp;Chih-Chia Cheng ,&nbsp;Hui-Ling Hsu ,&nbsp;Feng-Ping Lin ,&nbsp;Chien-Hsing Lu ,&nbsp;Jem-Kun Chen","doi":"10.1016/j.snb.2025.137364","DOIUrl":"10.1016/j.snb.2025.137364","url":null,"abstract":"<div><div>When body fluids come into contact with a fibrous mat, proteins spontaneously adsorb onto the surface, forming a protein corona that masks the original antibody on the surface and significantly reduces the targeting performance. Nylon6 (N6) containing curcumin and a cross-linked protein corona hydrogel were used as the shell and core, respectively, to prepare protein corona-camouflaged immunofiber mats (PIFMs) using coaxial electrospinning for the enrichment of circulating tumor cells (CTCs). A low dosage (1.2 wt%) of curcumin within the N6 reduces the outspread pseudopodia area and enhances the capture capacity of CTCs. The fibrous mat was adhered within a disposable microfluidic chip to investigate the capture capacities of CTCs. The maximum adsorption capacity of CTCs on the PIFMs loaded with curcumin is approximately 26 cells/mm², about 2-fold higher than that on the blank PIFMs (12 cells/mm²). The outer protein corona hydrogel coating not only effectively enhances the antibiofouling properties and cell viability but also significantly decreases hemolysis. In spiked blood samples, our PIFMs loaded with curcumin exhibited a 90.1 % isolation efficiency in a continuous system at a flow rate of 8 mL/hour from the 10 mL blood samples containing 100 CTCs. Our proposed method provides an ideal fibrous mat to embed within a microfluidic chip to analyze CTCs attachment behavior for precise diagnosis in cancer treatment.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137364"},"PeriodicalIF":8.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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