Rongshuai Che , Yu Ya , Zeping Wang , Bingtao Fu , Chenchen Jin , Defen Feng , Jun Yan , Ke-Jing Huang , Xuecai Tan , Jing Xu
{"title":"Built-in capacitive biosensor based leveraging rolling circle amplification-architected bimetallic nanozyme/glucose oxidase catalytic forest for overcoming sensitivity limits in plant pathogen monitoring","authors":"Rongshuai Che , Yu Ya , Zeping Wang , Bingtao Fu , Chenchen Jin , Defen Feng , Jun Yan , Ke-Jing Huang , Xuecai Tan , Jing Xu","doi":"10.1016/j.snb.2025.138977","DOIUrl":"10.1016/j.snb.2025.138977","url":null,"abstract":"<div><div>Sugarcane smut, responsible for 35–60 % yield losses and 18–22 % sugar content reductions, demands early diagnostic solutions to mitigate its agricultural impact. Current methods like qPCR remain constrained by limited sensitivity and reliance on centralized laboratory infrastructure. To address these challenges, a field-deployable biosensing platform is developed via the integration of three synergistic innovations: a rolling circle amplification-engineered catalytic “nanozyme forest” that achieves high-density immobilization of Au-Pt bimetallic nanozymes and glucose oxidase active sites; an entropy-driven DNA strand displacement amplification system employing multi-recognition probes for sequence-specific capture and signal amplification of the pathogen biomarker bE4’; and a dual-functional Au@CoV-MOF electrode that simultaneously enables pseudocapacitive energy storage and enhances interfacial electron transfer. The biosensor demonstrates a linear detection range from 10<sup>−16</sup> to 10<sup>−8</sup> M with a 24.86 aM detection limit, surpassing qPCR sensitivity by two orders of magnitude while maintaining single-base mismatch discrimination. Field validation using sugarcane samples shows complete diagnostic concordance with qPCR results but reduces assay time through elimination of nucleic acid extraction and thermal cycling requirements. By synergizing enzymatic cascade amplification with self-powered energy storage, this work establishes a new paradigm for plant pathogen diagnostics that bridges the gap between laboratory-grade sensitivity and field-deployable practicality.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138977"},"PeriodicalIF":3.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145289522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jian Chen , Jiaying Yang , Yanbin Lu , Zhiyuan Dai , Huan Li
{"title":"Dual-signal amplified SERS sensing based on aptamer-modified Au/Fe3O4 nanohybrids and Ag NPs-functionalized bacterial cellulose film for on-site marine toxin analysis","authors":"Jian Chen , Jiaying Yang , Yanbin Lu , Zhiyuan Dai , Huan Li","doi":"10.1016/j.snb.2025.138929","DOIUrl":"10.1016/j.snb.2025.138929","url":null,"abstract":"<div><div>Okadaic acid (OA), a diarrhetic shellfish toxin, poses severe health risks to humans and causes substantial economic losses in aquaculture, creating an urgent need for rapid, sensitive, and field-deployable detection technologies for tracing its contamination in shellfish. Herein, we proposed a surface-enhanced Raman scattering (SERS) sensing method for OA in shellfish. The system was constructed by integrating Raman-active aptamer-modified gold/magnetic nanohybrids (Apt-Au/Fe<sub>3</sub>O<sub>4</sub> NHs) with a complementary sequence-modified silver nanoparticle-functionalized bacterial cellulose film (CS-Ag NPs@BC-film). Upon the introduction of OA, it competitively bound with the Apt, leaving the CS-Ag NPs@BC-film after magnetic separation, thereby inducing a reduction in the Raman signal. Sensitive quantification of OA was achieved by measuring the difference in the Raman signal, achieving a detection limit as low as 2.82 × 10<sup>−2</sup> nM (S/N = 3). Analysis of the spiked real samples demonstrated good accuracy and precision of the method. Compared with commercial ELISA kits, the method presented with comparable detection results while offering simplified operational workflows. These findings highlight its potential as a practical tool for monitoring hazardous contaminants in aquatic environments and ensuring seafood safety.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138929"},"PeriodicalIF":3.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145334245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xinying Zhang , Yihang Li , Fuliang Wei , Yingying Chen , Zhenzhen Li , Lingyan Feng
{"title":"Stimuli-responsive DNA hydrogels releasing nucleoside carbon dots to enhance enzyme activity for cancer subtype discrimination","authors":"Xinying Zhang , Yihang Li , Fuliang Wei , Yingying Chen , Zhenzhen Li , Lingyan Feng","doi":"10.1016/j.snb.2025.138980","DOIUrl":"10.1016/j.snb.2025.138980","url":null,"abstract":"<div><div>Signal probe leakage poses a significant challenge in signal amplification methods for DNA hydrogel-based electrochemical biosensors. To address this issue, we developed a dual-trigger DNA hydrogel utilizing the i-motif structure, which not only ensures rapid response times (within 5 min) but also maintains the prolonged stability of the encapsulated signal probes (up to 120 h). To further enhance stability, we constructed a catalytic system where adenosine carbon dots (ACDs) modulate glucose dehydrogenase activity and functionalized as signal probes. We established detection pathways for three miRNAs and explored the relationship between analyte concentration and the biosensor's capability to discriminate among three types of exosomes. The results demonstrate that the combination of miRNA-21 and miRNA-122 achieved superior classification performance, with a balanced classification effect for exosomes across various concentrations (83.33 %). This study presents new opportunities for the design of stable electrochemical biosensors and provides valuable insights of multi-target detection to improve the analytical accuracy.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138980"},"PeriodicalIF":3.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145289524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qiwen Peng , Fangbing Wang , Yi Ru , Yongheng Zhang , Guoyue Shi , Min Zhang
{"title":"Laser-written nanometal-doped graphene on paper for Alzheimer’s multi-target sensing","authors":"Qiwen Peng , Fangbing Wang , Yi Ru , Yongheng Zhang , Guoyue Shi , Min Zhang","doi":"10.1016/j.snb.2025.138973","DOIUrl":"10.1016/j.snb.2025.138973","url":null,"abstract":"<div><div>Laser-induced graphene (LIG) is highly favored in the sensing field due to its outstanding physicochemical properties. However, conventional PI film substrates suffer from limited flexibility and weak adhesion, which causes LIG detachment and compromised sensing stability, restricting their sensing applications. Moreover, the traditional approach of doping metal nanoparticles into LIG through secondary laser processing faces challenges: scanning path deviations cause uneven distribution, while repeated laser exposure may damage the substrate. To overcome these limitations, we used one-step laser direct writing (LDW) to engrave PI paper pre-soaked in a metal chloride solution, directly inducing graphene formation and in-situ reducing metal nanoparticles (LIG-Metal) simultaneously. Using PI paper as the substrate enhances the electrode's flexibility and adhesion, thereby preventing detachment. The incorporation of metal nanoparticles greatly improves the sensitivity of paper-based LIG electrodes while expanding their modification potential for sensing applications. Additionally, plasma treatment further improves the hydrophilicity and stability of PI paper-based LIG electrodes. Using prepared gold nanoparticle-doped laser-induced graphene (LIG-Au) electrodes as extended gate (EG), we developed a portable, multi-target intelligent sensing system. This system enables real-time, wireless monitoring of peroxynitrite (ONOO<sup>−</sup>) and c-Abl dynamics in the brains of Alzheimer’s disease (AD) transgenic mice, while also facilitating multi-dimensional investigation of their interactions and pathological roles in AD progression.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138973"},"PeriodicalIF":3.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145288922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wisarut Khamcharoen , Jutamas Jaewjaroenwattana , Kwanchanok Wajasen , Sarida Naorungroj , Sakda Jampasa , Charles S. Henry , Anchaleeporn Waritswat Lothongkumand , Orawon Chailapakul
{"title":"Dip-to-detect electrochemical microfluidic device: Integrating sample collection and automated mixing for on-site detection of heavy metals","authors":"Wisarut Khamcharoen , Jutamas Jaewjaroenwattana , Kwanchanok Wajasen , Sarida Naorungroj , Sakda Jampasa , Charles S. Henry , Anchaleeporn Waritswat Lothongkumand , Orawon Chailapakul","doi":"10.1016/j.snb.2025.138931","DOIUrl":"10.1016/j.snb.2025.138931","url":null,"abstract":"<div><div>Human exposure to heavy metals poses a global threat due to their harmful health and environmental effects. Standard analytical methods rely on complex operations and sophisticated instruments, making them costly, labor-intensive, and limited in on-site suitability. This work presents a “dip-to-detect” electrochemical microfluidic device that enables direct sample dipping and automated electrolyte mixing. The device is made using stacked layers of transparent and double-sided adhesive films designed to enable sequential flow control. We implement a fluid-triggered valve to allow direct dipping and store the sample solution inside the channel without leakage. The electrolyte is added to the device without fixed-volume pipetting, activating the valve for rapid mixing with the sample solution. The device is equipped with a near field communication (NFC) controlled potentiostat for the portable detection of Cd(II) and Pb(II) with limits of detection of 90 ppb and 80 ppb, respectively. To validate its performance, the microfluidic device was tested with spiked industrial water samples. The device demonstrated good recovery, ranging from 102.2 % to 116.7 %. The dip-to-detect electrochemical microfluidic device eliminates the need for pipetting and manual mixing. This platform introduces a new alternative for field-deployable sensing and has the potential to inspire future designs focused on simple sample collection, automated reagent handling, and minimal user intervention.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138931"},"PeriodicalIF":3.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145327109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shu-Xuan Cui, Pan-Dong Mao, Yuan Wang, Lin-Yan Bian, Wei-Na Wu, Zhi-Hong Xu, Tony D. James
{"title":"Dual hypochlorite and adenosine-5’-triphosphate responsive ZIF-90 nanosystem and its application for biological imaging","authors":"Shu-Xuan Cui, Pan-Dong Mao, Yuan Wang, Lin-Yan Bian, Wei-Na Wu, Zhi-Hong Xu, Tony D. James","doi":"10.1016/j.snb.2025.138974","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138974","url":null,"abstract":"Hypochlorite ion (ClO<sup>−</sup>) and adenosine-5’-triphosphate (ATP) are crucial in various physiological functions. The connection between ClO<sup>−</sup> and ATP has been noted as one of the major characteristics of liver injury. This study presents a zeolitic imidazolate framework (ZIF-90)-based probe RhB@MP-ZIF-90 for the simultaneous identification of ClO<sup>−</sup> and ATP, which was fabricated by in situ encapsulation of rhodamine B (RhB) and successive modification of 4-methyl-1-(4-(4,4,5,5-tetramethyl-1,3,2-dioxa-borolan-2-yl)benzyl)pyridinium bromide (MP). A well-dispersed aqueous solution of this nano-scale probe is highly emissive in the green channel due to the charge transfer from ZIF-90 to the pyridinium. ClO<sup>−</sup> oxidizes the boronate ester group to hydroxyl in probe RhB@MP-ZIF-90, which is accompanied by the elimination of 4-methylene-cyclohexa-2,5-dienone, changing pyridinium to pyridine, blocking ICT, and quenching the green emission. By contrast, the ZIF-90 backbone of the probe is destroyed by ATP, releasing RhB to generate a characteristic red emission. Thus, this easy-to-prepare probe enabled the dual detection of ClO<sup>−</sup> and ATP, achieved through ClO<sup>−</sup>-triggered fluorescence decrease and ATP-induced fluorescence amplification in two independent channels, with detection limits of 24.94<!-- --> <!-- -->μM for ClO<sup>−</sup> and 360<!-- --> <!-- -->μM for ATP, respectively. As such, RhB@MP-ZIF-90 was successfully used to monitor concentration changes of mitochondrial ClO<sup>−</sup> and ATP in different biological processes and a drug-induced liver injury model.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"125 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xueling Dong , Yawen Wang , Chuang Du , Nana Ma , Shaopeng Gao , Ruinan Li , Jiaxin Bai , Xulin Liu , Run Ran , Yutao Xu , Dezhong Yang , Haikui Zou
{"title":"A smartphone-assisted platform integrated with a natural AIEE-active fisetin probe for visual detection of Mg²⁺ ions","authors":"Xueling Dong , Yawen Wang , Chuang Du , Nana Ma , Shaopeng Gao , Ruinan Li , Jiaxin Bai , Xulin Liu , Run Ran , Yutao Xu , Dezhong Yang , Haikui Zou","doi":"10.1016/j.snb.2025.138971","DOIUrl":"10.1016/j.snb.2025.138971","url":null,"abstract":"<div><div>Magnesium ions (Mg²⁺) play essential roles in physiological processes and water quality regulation, making their accurate detection crucial for environmental and water quality monitoring. However, rapid, portable, and user-friendly detection methods are still limited. Herein, we identified fisetin (Fis), a naturally occurring flavonol, as a colorimetric and fluorescent bio-resource derived aggregation-induced emission luminogen (BioAIEgen) probe for highly sensitive and selective Mg²⁺ detection. Fis exhibits excited-state intramolecular proton transfer (ESIPT)-induced aggregation-induced emission enhancement (AIEE) properties, with solvent-dependent photophysical behavior confirmed by density functional theory (DFT) calculations. Notably, Fis enables rapid (< 1 min) and selective detection of Mg²⁺ in a DMSO/H₂O mixture (water fraction, <em>f</em><sub>H₂O</sub> = 0.5 %, v/v) by forming a stable 2:3 butterfly-shaped Fis–Mg<sup>2 +</sup> complex, accompanied by a visible color change from colorless to light green (daylight) and a fluorescence shift from yellow to green (UV light). The detection limit (LOD) reaches 19.26 nM. Based on these properties, we developed a low-cost, portable, and user-friendly fluorescence sensing platform by integrating Fis-based test strips with smartphone-assisted red–green–blue (RGB) analysis. The test strips exhibited bright, concentration-dependent fluorescence responses to Mg²⁺, enabling both visual and quantitative detection with an LOD of 1.38 μM. This platform was successfully applied to river and glacial water, as well as commercial beverages, demonstrating its practical potential. The work presents a simple and effective strategy for Mg²⁺ monitoring in diverse aqueous environments.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138971"},"PeriodicalIF":3.7,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linjing Duan , Zhiguang Suo , Yaqi Yan , Jinmin Zhang , Rui Guo , Min Wei , Huali Jin , Renyong Zhao
{"title":"Simultaneous detection of tetracycline and kanamycin by electrochemical sensors based on dual-signal probes Au/Mn-based Prussian blue analogs and methylene blue-functionalized AuPt NFs","authors":"Linjing Duan , Zhiguang Suo , Yaqi Yan , Jinmin Zhang , Rui Guo , Min Wei , Huali Jin , Renyong Zhao","doi":"10.1016/j.snb.2025.138933","DOIUrl":"10.1016/j.snb.2025.138933","url":null,"abstract":"<div><div>Dual-signal probes Au/Mn-based Prussian blue analogs (Mn-PBA) and methylene blue (MB)-functionalized AuPt nanoflowers (AuPt NFs) were prepared, and an electrochemical aptasensor capable of simultaneously detecting tetracycline (TET) and kanamycin (Kana) was constructed using exonuclease III (Exo III)-assisted cycling. The combination of Mn-PBA and Au NPs resulted in Au/Mn-PBA, which exhibits high redox properties and can provide electrochemical signals for target detection. MB-modified AuPt NFs were used as another signaling probe. Their flower-like structure loads a large number of signaling molecules to provide electrochemical signals to the sensor. Magnetic beads were used to connect different DNA sequences, enabling simultaneous Exo III cycling of the supernatant and precipitate in the presence of target analytes. Under the optimal conditions, the linear range of TET and Kana was 0.001–500 ng mL⁻¹ . The limit of detection (LOD) of TET was 0.126 pg mL⁻¹ , and that of Kana was 0.933 pg mL⁻¹ . The limits of quantification (LOQs) of TET and Kana were 0.912 pg mL⁻¹ and 3.31 pg mL⁻¹ , respectively. In addition, the sensor successfully achieved simultaneous detection of TET and Kana in real samples, showing promising practical prospects.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138933"},"PeriodicalIF":3.7,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145261886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liyang Duan , Qian Chen , Songsong Huang , Zhi Yu , Xuexing Zhao , Chong Chen , Shuang Li , Haojun Fan , Bin Fan , Zetao Chen
{"title":"Battery-free wireless stretchable wearable sweat sensor with ion-driven optical signal transduction and chip-level discrete components","authors":"Liyang Duan , Qian Chen , Songsong Huang , Zhi Yu , Xuexing Zhao , Chong Chen , Shuang Li , Haojun Fan , Bin Fan , Zetao Chen","doi":"10.1016/j.snb.2025.138947","DOIUrl":"10.1016/j.snb.2025.138947","url":null,"abstract":"<div><div>Continuous wearable monitoring of sweat electrolytes can provide valuable insights into human metabolic dynamics. Existing strategies usually rely on integrated chips and complicated signal transductions, which is power consuming and limits wearable applications. Herein, we developed a wireless and battery-free stretchable sensor for the detection of multiple electrolytes in sweat simultaneously. Ion-selective electrodes and junction field-effect transistors (JFETs) were integrated for the converting of electrode potential changes triggered by sweat ion concentration variations into JFET channel current changes, of which the signal could be read out through the brightness changes of light-emitting diodes (LEDs). This sensor exhibits high sensitivity and linear responses in the ranges of 1–32 mM for K⁺, 5–160 mM for Na⁺, and 4–8 for pH. Leveraging a self-designed fast image processing algorithm, the detection accuracy is comparable to that of traditional standard instruments, ensuring the practicality of wearable applications. Notably, the sensor is ultra-low-power consumption, with a peak power of only 24.5–31.5 mW. By integrating microfluidic chips and fully elastic near field communication (NFC) power supply antenna, the full-process wearable detection strategy is achieved. Based on the correlation between sweat electrolytes and human health, this sensor presents an on-site detection platform with high accuracy and ultra-low-power consumption, providing a solution for health management accessible to families.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138947"},"PeriodicalIF":3.7,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145283436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khue Tian Lai , Jinal Tapar , Paul D. Harrison , Shuhao Wu , Maira Elksne , Chunxiao Hu , Vincenzo Pusino , David R.S. Cumming
{"title":"Paper-based short-wave infrared spectroscopy for glucose quantification with human serum","authors":"Khue Tian Lai , Jinal Tapar , Paul D. Harrison , Shuhao Wu , Maira Elksne , Chunxiao Hu , Vincenzo Pusino , David R.S. Cumming","doi":"10.1016/j.snb.2025.138963","DOIUrl":"10.1016/j.snb.2025.138963","url":null,"abstract":"<div><div>Accurate glucose monitoring is essential for diabetes management and clinical diagnostics. We present a paper-based assay utilizing a short-wave infrared (SWIR) spectroscopy for glucose quantification at the 6599 cm⁻¹ (1515 nm) absorption peak, while minimizing the water interference. Our study investigates two structural forms of glucose, α-D-glucose and β-D-glucose (anomers), tracking their ratio in glucose solutions with phosphate-buffered saline (PBS), pure human serum, and their mixtures. While the β/α D-glucose ratio remains stable across all measured glucose concentrations in PBS, human serum induces a shift favoring α-D-glucose at higher concentrations, due to glucose interactions with other biomolecules. Our findings may have implications for glucose transport, enzymatic processing, metabolic assessment and understanding nutrient uptake in the body. While SWIR glucose spectroscopy has been explored in prior studies, our work integrates anomeric equilibrium shifts, protein-glucose binding effects, and paper-based sensing into a unified analytical framework, providing an effective approach for glucose quantification with wide-ranging applications in human health.</div></div><div><h3>Teaser</h3><div>This study explores a simpler, accurate and cost-effective method to test glucose with human serum using infrared light.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"448 ","pages":"Article 138963"},"PeriodicalIF":3.7,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145261331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}