Sensors and Actuators B: Chemical最新文献_第4页

Improved ensemble learning enhanced serum fingerprinting spectroscopy for lung cancer diagnosis

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-03 DOI: 10.1016/j.snb.2025.137353

Zhejun Yang , Ren Zhang , Chenlei Cai , Hua Zhang , Hui Chen , Jilie Kong

{"title":"Improved ensemble learning enhanced serum fingerprinting spectroscopy for lung cancer diagnosis","authors":"Zhejun Yang , Ren Zhang , Chenlei Cai , Hua Zhang , Hui Chen , Jilie Kong","doi":"10.1016/j.snb.2025.137353","DOIUrl":"10.1016/j.snb.2025.137353","url":null,"abstract":"<div><div>The early diagnosis of lung cancer is crucial for improving patient prognosis, and liquid biopsy plays an important role in early lung cancer screening. In the field of liquid biopsy, label-free Surface-Enhanced Raman Scattering (SERS) possesses its unique advantages as it can provide comprehensive information about the insights into the chemical makeup of serum. However, the nonuniform SERS substrates poses challenges for reliable clinical diagnosis. We design a Surface Controlled SERS with Improved Ensemble Learning (<em>SCSIEL</em>) for lung cancer screening and diagnosis. This finely controlled SERS substrate ensures the uniformity of the surface and the high reproducibility of SERS spectra. The improved ensemble learning used in <em>SCSIEL</em> consists of a multi-layer structure which is inspired from the residual connection of deep learning networks. Although the framework is lightweight and integrates only a few simple base models, it achieves impressive results under the carefully constructed network. This <em>SCSIEL</em> system is also validated by direct analysis of clinical serum samples from 168 lung cancer patients and 100 healthy controls and the excellent performance is obtained with an area under the curve (AUC) of 97.0 % and accuracy of 93.4 %, which outperforms that of the clinical biomarkers for lung cancer. This <em>SCSIEL</em> is also explainable, which indicates the enhanced protein degradation in lung cancer. The <em>SCSIEL</em> method is a reliable and cost-effective method in the screen of lung cancer, shows great promise in clinical implementation.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137353"},"PeriodicalIF":8.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A target-triggering strategy for self-powered biosensor based on chemical nanogenerator

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-03 DOI: 10.1016/j.snb.2025.137361

Xinqi Luo, Hanxiao Chen, Ge Ling, Wenbo Yue, Bingbing Xie, Kexin Guo, Jing Xu

{"title":"A target-triggering strategy for self-powered biosensor based on chemical nanogenerator","authors":"Xinqi Luo, Hanxiao Chen, Ge Ling, Wenbo Yue, Bingbing Xie, Kexin Guo, Jing Xu","doi":"10.1016/j.snb.2025.137361","DOIUrl":"10.1016/j.snb.2025.137361","url":null,"abstract":"<div><div>The self-powered sensing platform uses redox reactions to convert chemical nanogenerators into electrical energy, powering the system for target detection. In this work, a multi-dimensional hollow C@SnO<sub>2</sub> nanocomposite is designed as the electrode substrate and combined with the catalytic hairpin assembly (CHA) amplification strategy. This platform enables highly sensitive detection of the breast cancer marker miRNA-145. C@SnO<sub>2</sub> provides a large specific surface area and excellent electron transport properties, offering abundant enzyme active sites and improving electron transport efficiency. In the presence of miRNA-145, the CHA reaction cycle is triggered, anchoring CHA products to the electrode surface. These signal molecules then participate in the electrochemical reaction, amplifying the signal. Under optimized conditions, the platform demonstrates a linear response range from 1 fM to 10 nM, with a detection limit as low as 0.78 fM. The self-powered sensor enabling sensitive detection of miRNA-145 at low concentrations which is crucial for the early diagnosis of breast cancer. Additionally, this work integrates the self-powered sensing platform with commercial chips, ensuring stable performance and minimal signal fluctuations during long-term continuous monitoring, enabling portable and real-time target monitoring. This design expands the application range of self-powered biosensors and offers new approaches for field detection of other targets.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137361"},"PeriodicalIF":8.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light/ultrasound enhance peroxidase activity of BaTiO3/graphdiyne/Au nanozyme for colorimetric detection of E. coli O157:H7

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-02 DOI: 10.1016/j.snb.2025.137378

Di Zhang , Haoxin Li , Kai Wang , Yujian Sun , Chenguang Wang , Yingying Wang , Qiang Bai , Tianzhi Yu , Zhugen Yang , Ning Sui , Lina Wang

{"title":"Light/ultrasound enhance peroxidase activity of BaTiO3/graphdiyne/Au nanozyme for colorimetric detection of E. coli O157:H7","authors":"Di Zhang , Haoxin Li , Kai Wang , Yujian Sun , Chenguang Wang , Yingying Wang , Qiang Bai , Tianzhi Yu , Zhugen Yang , Ning Sui , Lina Wang","doi":"10.1016/j.snb.2025.137378","DOIUrl":"10.1016/j.snb.2025.137378","url":null,"abstract":"<div><div>In the past two decades, nanozymes have garnered increasing interest, however, their catalytic activity and efficacy still lag significantly behind that of natural enzymes, posing limitations on their utility in bioanalytical applications. In this study, we introduced a novel BaTiO<sub>3</sub>/graphdiyne/Au (BGA) nanozyme that leverages surface plasmon resonance and piezoelectric effects to concurrently respond to light and ultrasound (US) stimulation, resulting in a 3.8-fold enhancement in peroxidase-like activity. Theoretical and experimental findings suggest that US stimulation induces lattice distortion in BaTiO<sub>3</sub>, leading to the reversible conversion of C<img>C bonds to C<img>C bonds in graphdiyne. Consequently, the liberated electrons recombine with the hot holes produced by Au nanoparticles upon light excitation, thereby efficiently inhibiting the recombination of hot electron-hole pairs and substantially augmenting peroxidase-like activity. The BGA nanozyme was further configured as a detection platform for <em>E. coli</em> O157:H7. The sensor exhibited a broad linear range (1–10<sup>7</sup> CFU mL<sup>−1</sup>) and a low limit of detection of 7 CFU mL<sup>−1</sup>. Moreover, the sensor exhibited exceptional applicability in the analysis of various real samples such as milk and lemon juice. This study presents a novel research framework for constructing high-activity nanozyme sensors responsive to external fields, offering significant potential in biological analysis, environmental surveillance, and food safety applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137378"},"PeriodicalIF":8.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thianthrene-based heavy metal-free oxygen analytes exhibiting room temperature phosphorescence and high sensitivity for low oxygen concentration

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-02 DOI: 10.1016/j.snb.2025.137380

Lukas Dvylys , Rasa Keruckiene , Matas Guzauskas , Shi-Wei Chu , Juozas V. Grazulevicius

引用次数: 0

PEI doped In2O3 nanospheres based gas sensor for high-performance formaldehyde detection

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-01 DOI: 10.1016/j.snb.2025.137374

Xiaoxue Ma, Degen Chen, Weichao Li, Lifang He, Ling Jin, Jian Zhang, Kui Zhang

{"title":"PEI doped In2O3 nanospheres based gas sensor for high-performance formaldehyde detection","authors":"Xiaoxue Ma, Degen Chen, Weichao Li, Lifang He, Ling Jin, Jian Zhang, Kui Zhang","doi":"10.1016/j.snb.2025.137374","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137374","url":null,"abstract":"Doping metal oxides with proper polymers would be another effective strategy to address their gas sensing shortcomings, such as low sensitivity and unsatisfactory detection limits in certain applications. Herein, polyethyleneimine (PEI) with its high thermal stability and special functional groups was employed to dope In<sub>2</sub>O<sub>3</sub> using a facile hydrothermal method followed by calcination. The resultant In<sub>2</sub>O<sub>3</sub>/PEI nanospheres (PIn) composites sensors exhibited improved selectivity and sensitivity to formaldehyde. Specifically, the PIn composite sensor with 5 wt% PEI (PIn-5%) showed a high response value of 420.1 to 105 ppm formaldehyde at 110℃, which was about four times higher than that of bare In<sub>2</sub>O<sub>3</sub> and twice that of the mixture of In<sub>2</sub>O<sub>3</sub> and PEI. The composite sensor also demonstrated good response linearity, low detection limit and fast response. To demonstrate its potential application in food quality inspection, the composite sensor was employed in rapid detection of low concentrations of residual formaldehyde on vegetables, revealing that formaldehyde might be misused as a preservative in some cases. Finally, the gas sensing mechanism of PIn composites was discussed, and their improved formaldehyde sensing performance was attributed to the heterojunctions formed between PEI and In<sub>2</sub>O<sub>3</sub>, the specific selective interaction of PEI with formaldehyde, the increased oxygen vacancy, and the porous structure with an enlarged surface area and small In<sub>2</sub>O<sub>3</sub> nanoparticle size. These results suggested a promising approach to enhancing the gas sensing performance of metals oxides using appropriate polymers.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"60 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Target-activated CRISPR/Cas12a recognize multifunctional G-quadruplex and dual fluorescent indicators enable rapid non-extraction analysis of circulating tumor DNA in breast cancer

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-01 DOI: 10.1016/j.snb.2025.137372

Xin Zhao , Weijing Liu , Runlian Qu , Xin Jiang , Jie Chen , Piaopiao Chen

{"title":"Target-activated CRISPR/Cas12a recognize multifunctional G-quadruplex and dual fluorescent indicators enable rapid non-extraction analysis of circulating tumor DNA in breast cancer","authors":"Xin Zhao , Weijing Liu , Runlian Qu , Xin Jiang , Jie Chen , Piaopiao Chen","doi":"10.1016/j.snb.2025.137372","DOIUrl":"10.1016/j.snb.2025.137372","url":null,"abstract":"<div><div>A simple, efficient, and cost-effective method for identifying extremely low levels of circulating tumor DNA (ctDNA) is crucial for the diagnosis and monitoring of cancer. Herein, we designed a label-free and one-pot assay for accurate detection of ctDNA with the combination of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a system, G-quadruplex (G4), and dual-signal reporters, polydopamine (PDA) and N-Methyl Mesoporphyrin IX (NMM). Without ctDNA extraction, CRISPR/Cas12a could directly recognize the target double-stranded ctDNA and activate the Cas12a protein. G4 could bind to NMM/K<sup>+</sup> to enhance its fluorescence signal, and combine with hemin/K<sup>+</sup> to form a complex that catalyzed dopamine to form PDA and exhibit an intense signal. When the target ctDNA was introduced, the trans-cleavage of G4 by activated Cas12a caused a significant signal decrease in NMM and PDA, acting as a signal amplifier. This assay could detect ctDNA as low as 4 aM in 40 min and distinguish single-base mutations. The CRISPR/Cas12a-G4 biosensor, validated on 48 samples, achieved a specificity of 100 %, a sensitivity of 92 %, and an area under the receiver operating characteristic curve (AUC) of 0.983. These findings were consistently supported by clinical data (imaging results, pathological assessments), and quantitative real-time polymerase chain reaction (qRT-PCR) data. The biosensor's robust performance and potential for application in low-resource settings make it a promising tool for advancing cancer diagnostics and management.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137372"},"PeriodicalIF":8.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing surface states to elevate xylene gas sensing characteristics in ZnCo2O4 microspheres

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-01 DOI: 10.1016/j.snb.2025.137369

Chenlu Hu , Rui Jiang , Yanxu Feng , Jie Huo , Bosen Zhang , Shuangming Wang , Jing Cao

引用次数: 0

Multi-component gas sensing via spectral feature engineering

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-01 DOI: 10.1016/j.snb.2025.137285

Mohamed Sy , Sarah Aamir , Aamir Farooq

{"title":"Multi-component gas sensing via spectral feature engineering","authors":"Mohamed Sy , Sarah Aamir , Aamir Farooq","doi":"10.1016/j.snb.2025.137285","DOIUrl":"10.1016/j.snb.2025.137285","url":null,"abstract":"<div><div>We present a straightforward yet powerful spectral feature engineering technique designed to improve multi-species detection in complex mixtures. By applying convolutions of first derivatives with the composite spectra of target species before feeding the data into a convolutional neural network (CNN) model, this method significantly enhances the detection of weak absorbers and overlapping spectral features. To validate the approach, we developed a laser-based sensor that integrates wavelength tuning with a 1-D CNN model. The system utilizes a distributed feedback inter-band cascade laser operating near <span><math><mrow><mn>3</mn><mo>.</mo><mn>34</mn><mspace></mspace><mi>μ</mi><mi>m</mi></mrow></math></span>, enabling selective and simultaneous measurement of <span><math><mrow><msub><mrow><mi>C</mi></mrow><mrow><mn>1</mn></mrow></msub><mo>−</mo><msub><mrow><mi>C</mi></mrow><mrow><mn>3</mn></mrow></msub></mrow></math></span> hydrocarbons. Experiments were conducted at ambient conditions with a temporal resolution of 10 ms, while (intentionally) keeping the signal-to-noise ratio at relatively low levels. Gaseous mixtures contained methane, ethane, propane and propyne ranging in mole fraction values of 0%–1%, and ethylene mole fraction below 200 ppm. Ethylene was deliberately kept at very low levels to demonstrate the effectiveness of the feature engineering technique in detecting a weak absorbing species. The proposed method reduced the mean squared error by ten times compared to standard models. This demonstrates its potential for accurate detection in challenging environments.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137285"},"PeriodicalIF":8.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anionic heptamethine cyanine as reactive sulfur species-activated probe: Application of NIR-II fluorescence imaging for in vivo visualization of glutathione

IF 8 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-01 DOI: 10.1016/j.snb.2025.137371

Yu Liu , Deyang Kong , Junzheng Song , Zhao Wang , Yurong Guo , Lei Yu , Xing Gao , Yen Leng Pak , Jibin Song , Juyoung Yoon

{"title":"Anionic heptamethine cyanine as reactive sulfur species-activated probe: Application of NIR-II fluorescence imaging for in vivo visualization of glutathione","authors":"Yu Liu , Deyang Kong , Junzheng Song , Zhao Wang , Yurong Guo , Lei Yu , Xing Gao , Yen Leng Pak , Jibin Song , Juyoung Yoon","doi":"10.1016/j.snb.2025.137371","DOIUrl":"10.1016/j.snb.2025.137371","url":null,"abstract":"<div><div>Understanding glutathione (GSH)-related pathophysiological mechanisms requires their effective visualization. Current fluorescence probes for GSH visualization that operate in the visible and near-infrared (NIR-I) windows suffer from limited penetration in tissue and significant tissue autofluorescence. As part of this study, a GSH-activatable second near-infrared (NIR-II) fluorescent probe (denoted as <strong>T2S2</strong>) was developed to visualize GSH <em>in vivo</em> in a highly sensitive and selective manner. <strong>T2S2</strong> contains an anionic polymethylcyanide framework coupled to a 3,4-dimethoxybenzenethiol fluorescence quenching moiety specifically activated by GSH. In response to this activation, the NIR-II fluorescence is significantly enhanced, enabling the effective discrimination of GSH from similar biothiols with low molecular weight. The GSH detection limit of <strong>T2S2</strong> was established to be 90 nM, accompanied by a color change from blue-green to yellow-green. Notably, the <strong>T2S2</strong> probe was found to be highly selective and sensitive to GSH <em>in vivo</em>. Hence, this study presents a promising approach for developing activatable NIR-II fluorescent probes to image GSH and other biomarkers <em>in vivo</em>.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137371"},"PeriodicalIF":8.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing Ag-Decorated Electrospun Ta-doped CeO2 Nanofibers for Superior Acetone Detection with Unparalleled Humidity Resistance

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-02-01 DOI: 10.1016/j.snb.2025.137367

Xinge Wang, Wenjian Zhang, Haiqing Jiang, Xukun Wang, Zhen Miao, Wei Wei, Yuhao Zhang, Yuan Lin, Tianyu Wu, Guo Liu, Meixia Su, Yan Ding, Kun Tao, Jinyuan Zhou, Erqing Xie, Juan Li, Zhenxing Zhang

{"title":"Harnessing Ag-Decorated Electrospun Ta-doped CeO2 Nanofibers for Superior Acetone Detection with Unparalleled Humidity Resistance","authors":"Xinge Wang, Wenjian Zhang, Haiqing Jiang, Xukun Wang, Zhen Miao, Wei Wei, Yuhao Zhang, Yuan Lin, Tianyu Wu, Guo Liu, Meixia Su, Yan Ding, Kun Tao, Jinyuan Zhou, Erqing Xie, Juan Li, Zhenxing Zhang","doi":"10.1016/j.snb.2025.137367","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137367","url":null,"abstract":"The imperative to detect acetone (CH<sub>3</sub>COCH<sub>3</sub>) in the absence of humidity interference is underscored by its expansive utility in environmental and healthcare contexts. To address this, a novel sensor comprising Ag-decorated Ta-doped CeO<sub>2</sub> (STC) hollow nanofibers has been engineered through a meticulous process of electrospinning, followed by the decoration of Ag nanoparticles via UV irradiation. The fabricated STC-2 sensor (1 mol% Ag @ Ta-doped CeO<sub>2</sub>) presents a remarkable response of 36.6 to 100 ppm acetone at 170℃, 15.3-fold superior to that of its pristine CeO<sub>2</sub> counterpart, fast response time (19.53 s), exceptional selectivity, repeatability, and long-term stability. Impressively, the STC-2 sensor maintains a considerable response of 2.0 to acetone even at a minimal level of 0.5 ppm, with the theoretical lowest of detection limit of 9.9 ppb. In addition, the STC-2 sensor is resistant to moisture, attributed to the hydrophobic Ag (110) nanoparticles and the innovative \"U-shaped\" groove heterojunction which forms a potential barrier between between CeO<sub>2</sub> and Ag as well as Ta<sub>2</sub>O<sub>5</sub> and Ag. Meanwhile, density functional theory calculations affirm the STC sensors' predilection for high O<sub>2</sub> and acetone adsorption energy while maintaining low affinity to H<sub>2</sub>O molecules. These findings herald the potential of \"U-shaped\" groove heterojunction in advancing humidity-resistant gas sensors.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"125 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0