Sensors and Actuators B: Chemical最新文献_第4页

Thread-based colorimetric biosensor for urea determination in serum 血清中尿素测定用丝线比色生物传感器

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-09 DOI: 10.1016/j.snb.2026.139644

Izabela Lewińska , Paweł Bącal , Ignacio de Orbe-Payá , Luis Fermín Capitán-Vallvey , Miguel M. Erenas

{"title":"Thread-based colorimetric biosensor for urea determination in serum","authors":"Izabela Lewińska , Paweł Bącal , Ignacio de Orbe-Payá , Luis Fermín Capitán-Vallvey , Miguel M. Erenas","doi":"10.1016/j.snb.2026.139644","DOIUrl":"10.1016/j.snb.2026.139644","url":null,"abstract":"<div><div>Urea is a crucial biomarker in clinical analysis, mainly used to diagnose and monitor kidney condition. However, its reliable determination in serum in point-of-care testing format still remains a vital challenge. Here, we tackled this issue by developing a microfluidic thread-based optical biosensor for serum urea determination. The working principle of the presented device was combining enzymatic urea hydrolysis using urease with the resulting ammonium ions detection using ionophore-chromoionophore chemistry. Urease was immobilized on thread with the aid of synthesized urease-calcium phosphate nanoflowers while components for ammonium ions detection were embedded in PVC-based membrane located on the same thread. In the first step, ammonium ions determination in a thread-based sensor was optimized. Then, urease-calcium posphate nanoflowers (U-CaNFs) were included on the thread to ensure selectivity for urea. U-CaNFs synthesis was optimized and the resulting nanoflowers were characterized using various analytical techniqes (e.g. SEM, EDS, TGA, XRD). The calculated limit of detection for urea was 37 µmol L<sup>−1</sup> and the total analysis time was only 8 min. The developed thread-based devices were validated with control sera samples, proving their high accuracy.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139644"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146146725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photo-bioenergy-driven photocatalytic nanozyme systems for high-efficiency self-powered sensing 用于高效自供电传感的光生物能源驱动的光催化纳米酶系统

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-10 DOI: 10.1016/j.snb.2026.139643

Yue Ye , Qian Cui , Ting Bao , Junlun Zhu , Wei Wen , Xiuhua Zhang , Shengfu Wang

{"title":"Photo-bioenergy-driven photocatalytic nanozyme systems for high-efficiency self-powered sensing","authors":"Yue Ye , Qian Cui , Ting Bao , Junlun Zhu , Wei Wen , Xiuhua Zhang , Shengfu Wang","doi":"10.1016/j.snb.2026.139643","DOIUrl":"10.1016/j.snb.2026.139643","url":null,"abstract":"<div><div>Self-powered electrochemical sensors (SPESs), as a class of sensing devices featuring fast response times and external power-free operation, have demonstrated broad application prospects. To enhance the output performance and sensitivity of SPES, a novel photocatalytic nanozyme self-powered electrochemical sensor (PN-SPES) was developed by integrating photocatalytic fuel cells and enzymatic biofuel cells to synergistically utilize light energy and bioenergy. In this work, a pivotal antibiotic resistance determinant (mecA gene) is used as a model target to explore the analytical performance of PN-SPES. Firstly, a catalytic hairpin assembly DNA cycling amplification strategy and steric hindrance effects from DNA nanoflowers were used to achieve efficient signal amplification. A g-C<sub>3</sub>N<sub>4</sub>/SnO<sub>2</sub> photoanode was synthesized using elemental doping and heterojunction engineering, combining photocatalytic activity with enzyme-mimetic properties. SPES generates high output signals through the enzymatic and photocatalytic properties of photocatalytic nanozymes. Experimental results demonstrated that the constructed SPES exhibited a 130 mV higher electrochemical signal under light illumination compared to dark conditions in glucose solution, which was 1.4 times the output signal observed in the dark. A robust linear relationship was observed for mecA gene concentrations ranging from 10 fmol L<sup>−1</sup> to 10 nmol L<sup>−1</sup>, with a detection limit of 1.01 fmol L<sup>−1</sup>. This work provides new ideas for self-powered sensors to enhance the reliability and continuity of energy acquisition and implements a multi-energy collection strategy that can sensitively detect antibiotic resistance genes.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139643"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146153228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid screening of paratope-targeting peptides for the detection of anti-PD-1 monoclonal antibodies 检测pd -1单克隆抗体的paratope靶向肽的快速筛选

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-12 DOI: 10.1016/j.snb.2026.139659

Yu Chen , Zhe Zhang , Yuxin Zhang , Meiyu Lin , Panpan Chen , Chengxin Su , Yang Xia , Wenyu Liu , Yifeng Chai , Fang Fan , Zhuo Wang , Xiaofei Chen

{"title":"Rapid screening of paratope-targeting peptides for the detection of anti-PD-1 monoclonal antibodies","authors":"Yu Chen , Zhe Zhang , Yuxin Zhang , Meiyu Lin , Panpan Chen , Chengxin Su , Yang Xia , Wenyu Liu , Yifeng Chai , Fang Fan , Zhuo Wang , Xiaofei Chen","doi":"10.1016/j.snb.2026.139659","DOIUrl":"10.1016/j.snb.2026.139659","url":null,"abstract":"<div><div>Immune checkpoint blockade targeting PD-1 has emerged as a frontline therapy for diverse cancers, yet patient responses to anti-PD-1 monoclonal antibodies (mAbs) such as nivolumab and tislelizumab remain highly variable. These differences are closely linked to drug exposure, which affects both therapeutic benefit and adverse outcomes; however, the pharmacokinetic profiles of these antibodies are not well defined. This highlights the need for therapeutic drug monitoring (TDM) to guide precision immunotherapy. Yet, the development of rapid and reliable TDM methods is hindered by the lack of highly specific paratope-targeting biorecognition elements. In this study, we established a positive/negative F(ab')₂-based phage display screening strategy to efficiently identify paratope-targeting peptides for anti-PD-1 mAbs. Using this approach, we identified both cyclic and linear peptides, with two candidates showing good affinity and specificity toward anti-PD-1 mAbs while exhibiting minimal IgG binding. Cyclic peptides demonstrated higher specificity than their linear counterparts. Building on these peptides, we further developed a gold nanoparticle (AuNP)-based point-of-care detection platform that produced synchronized colorimetric signals, enabling simple, rapid, and accurate detection of anti-PD-1 mAbs. Application to clinical samples confirmed good accuracy compared to conventional ELISA. This strategy offers an effective tool for peptide screening and TDM of anti-PD-1 mAbs; it provides a broadly applicable framework for monitoring other therapeutic antibodies in clinical practice.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139659"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146160612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated solution-gated graphene transistor system for point-of-care ultrasensitive detection of exosomal mirna in tumor diagnosis 一种集成的溶液门控石墨烯晶体管系统，用于肿瘤诊断中外泌体mirna的即时超灵敏检测

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-05 DOI: 10.1016/j.snb.2026.139604

Yunhua Yang , Wanlei Gao , Renhao Ni , Panhui Yang , Yan Li , Ji Gao , Haojie Chen , Shengkang Lu , Zhiyong Guo , Fang Bao , Hui Cong , Qinghui Jin , Shilun Feng , Jianlong Zhao

{"title":"An integrated solution-gated graphene transistor system for point-of-care ultrasensitive detection of exosomal mirna in tumor diagnosis","authors":"Yunhua Yang , Wanlei Gao , Renhao Ni , Panhui Yang , Yan Li , Ji Gao , Haojie Chen , Shengkang Lu , Zhiyong Guo , Fang Bao , Hui Cong , Qinghui Jin , Shilun Feng , Jianlong Zhao","doi":"10.1016/j.snb.2026.139604","DOIUrl":"10.1016/j.snb.2026.139604","url":null,"abstract":"<div><div>Exosomal miRNAs are promising tumor biomarkers and are closely associated with clinical factors such as patient prognosis and chemotherapy sensitivity. However, their short sequences, low abundance, and dependence on bulky, non-portable instruments limit their application in rapid early screening. Thus, we developed a portable solution-gated graphene transistor (SGGT)-based detection system for tumor-derived exosomal miRNAs, specifically designed for point-of-care testing (POCT). The system integrates weak-signal excitation and acquisition modules and supports dual-mode operation, including transfer characteristic analysis and real-time current monitoring. The device is compact, portable, and enables direct result visualization, while the sensors support batch fabrication and facile functionalization with ssDNA probes on the SGGT gate. Leveraging the electric double-layer gating effect, this system achieved an ultra-low detection limit of 6.17 × 10⁻¹ ⁹ M, enabled miRNA-21 detection within 400 s, and effectively discriminated single-base mismatched sequences. It successfully distinguished exosomal miRNA-21 derived from tumor cells (H1975) and normal cells (HUVECs), consistent with the quantitative reverse transcription polymerase chain reaction(qRT-PCR) results and further differentiated samples from lung cancer patients and healthy individuals. Overall, the POCT system demonstrated rapid response, high sensitivity, and reliable performance, providing a practical platform for portable tumor biomarker detection and clinical diagnostics.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139604"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A self-priming-driven transcription amplification for single-molecule profiling of long noncoding RNAs in breast tissues 乳腺组织中长链非编码rna单分子分析的自引物驱动转录扩增

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-11 DOI: 10.1016/j.snb.2026.139640

Peng-fei Chu , Dongling Li , Juan Hu, Chun-yang Zhang

{"title":"A self-priming-driven transcription amplification for single-molecule profiling of long noncoding RNAs in breast tissues","authors":"Peng-fei Chu , Dongling Li , Juan Hu, Chun-yang Zhang","doi":"10.1016/j.snb.2026.139640","DOIUrl":"10.1016/j.snb.2026.139640","url":null,"abstract":"<div><div>Long noncoding RNAs (lncRNAs) act as vital regulators to influence gene expression, and aberrant lncRNA expression is linked to the onset and progression of various cancers. Herein, we develop a self-priming-driven transcription amplification for single-molecule profiling of long noncoding RNAs in breast tissues. This assay involves only one multifunctional hairpin probe that consists of four regions including a recognition portion of target, a T7 promoter complementary domain, a self-priming domain, and a transcription template domain. When target lncRNAs are absent, the self-priming region is locked within the hairpin probe and unable to trigger the subsequent polymerase-transcription amplification reaction. In the presence of lncRNAs, they specifically bind to the recognition portions of hairpin probes, inducing the intramolecular conformational switch of hairpin probes to rearrange the self-priming region. With the assistance of KF DNA polymerase (KF DNAP) and T7 RNA polymerase (T7 RNAP), target recycling polymerization-driven transcription generates abundant RNA transcripts. These transcripts then initiate the DSN-assisted cyclic cleavage of signal probes, releasing numerous Cy3/Cy5 fluorophores and producing enhanced Cy3 and Cy5 fluorescence signals. Leveraging the high efficiency of T7 RNAP/DSN-assisted cyclic cleavage reaction, this method exhibits high sensitivity with a detection limit of 34.2 aM for lncRNA HOTAIR and 42.2 aM for lncRNA MALAT1. Moreover, it can accurately quantify multiple endogenous lncRNAs at single-cell level, and efficiently discriminate the lncRNA expression levels in breast cancer tissues and healthy counterparts, offering a new platform for breast cancer diagnostics.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139640"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible eutectohydrogel sensor with structural color and stable electrical response under extreme conditions 具有结构颜色和极端条件下稳定电响应的柔性共结构水凝胶传感器

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-11 DOI: 10.1016/j.snb.2026.139637

Ling Bai , Huiqi Sun , Peipei Wang , Yingying Feng , Yuansheng Zeng , Rongguo Wang , Sai Wang , Fan Yang , Xiaodong He

{"title":"Flexible eutectohydrogel sensor with structural color and stable electrical response under extreme conditions","authors":"Ling Bai , Huiqi Sun , Peipei Wang , Yingying Feng , Yuansheng Zeng , Rongguo Wang , Sai Wang , Fan Yang , Xiaodong He","doi":"10.1016/j.snb.2026.139637","DOIUrl":"10.1016/j.snb.2026.139637","url":null,"abstract":"<div><div>Flexible strain sensors capable of stable operation in extreme environments are essential for next-generation wearable electronics and soft robotics. However, conventional hydrogels typically fail under harsh conditions because of freezing, dehydration, or hygroscopic swelling. In this work, we developed a flexible eutectohydrogel (EHG) sensor with structural color and stable electrical response by leveraging hydrogen bonding interactions between deep eutectic solvents (DES) and water. Polyvinylpyrrolidone<img>tannic acid<img>modified magnetite (Fe<sub>3</sub>O<sub>4</sub>@TA-PVP) colloid nanocrystal clusters (CNCs) assemble into a photonic crystal array under an oriented magnetic field, facilitating vivid and strain-responsive structural color changes. Concurrently, the PAM/Gelatin network exhibits high transparency, enabling undistorted transmission of optical signals through the gel matrix. The DES<img>water hydrogen bond network effectively prevents freezing and dehydration, maintaining the flexibility of the EHG and electrical responsiveness even at −40 °C. After seven days of exposure to 30 %<img>90 % relative humidity, the EHG retains stable optical output without moisture loss or hygroscopic swelling. Furthermore, the sensor exhibits an extremely low detection limit (0.5 %), rapid response time (0.83 s), and durable performance exceeding 1000 cycles. This research provides a reliable strategy for designing hydrogel sensors that adapt to environmental changes with dual-response capabilities, offering insights into developing flexible devices capable of reliable operation under extreme environmental conditions.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139637"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic anti-tumor effects of paclitaxel and autophagy inhibition in drug-resistant tumors revealed by ESIPT-based NIR real-time lysosomal imaging 基于esipt的近红外实时溶酶体成像揭示紫杉醇和自噬抑制在耐药肿瘤中的协同抗肿瘤作用

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-15 Epub Date: 2026-02-06 DOI: 10.1016/j.snb.2026.139616

Hengwei Sun, Jing Wen, Yiyu Zhu, Jinling Sun, Yefeng Cai, Jinmiao Qu

{"title":"Synergistic anti-tumor effects of paclitaxel and autophagy inhibition in drug-resistant tumors revealed by ESIPT-based NIR real-time lysosomal imaging","authors":"Hengwei Sun, Jing Wen, Yiyu Zhu, Jinling Sun, Yefeng Cai, Jinmiao Qu","doi":"10.1016/j.snb.2026.139616","DOIUrl":"10.1016/j.snb.2026.139616","url":null,"abstract":"<div><div>Acquired resistance to paclitaxel (PTX) in cancers such as breast cancer often involves “protective autophagy”, which helps tumor cells survive treatment. However, how the lysosomal microenvironment changes during resistance remains unclear, largely because tools for real-time lysosomal tracking in living systems are limited. To solve this, we designed a near-infrared fluorescent probe, Lyso-WY-Vis, based on an excited-state intramolecular proton transfer mechanism. It emits at 695 nm with a large Stokes shift (275 nm), enabling deep tissue penetration and high contrast. The probe incorporates a molecular rotor sensitive to viscosity and a morpholine unit for precise lysosome targeting. Employing this advanced tool, markedly elevated autophagy levels, both basal and PTX-induced, were observed in PTX-resistant cells (4T1/PTX), in contrast to the sensitive 4T1 cells. Furthermore, <em>in vivo</em> investigations revealed that combination therapy with PTX and 3-MA yielded robust anti-tumor effects, significantly inhibiting tumor proliferation and fully reversing the resistant phenotype in both sensitive and resistant tumor-bearing mouse models.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139616"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High sensitivity open seven-channel multipass cell TDLAS system for trace gas online monitoring 用于痕量气体在线监测的高灵敏度开放七通道多通单元TDLAS系统

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-01 Epub Date: 2026-02-04 DOI: 10.1016/j.snb.2026.139553

Le Yang , Haotian Yuan , Hongfu Fang , Zhiheng Gao , Rende Ma , Yue Pan , Xuzhen Gao , Mingsheng Niu

{"title":"High sensitivity open seven-channel multipass cell TDLAS system for trace gas online monitoring","authors":"Le Yang , Haotian Yuan , Hongfu Fang , Zhiheng Gao , Rende Ma , Yue Pan , Xuzhen Gao , Mingsheng Niu","doi":"10.1016/j.snb.2026.139553","DOIUrl":"10.1016/j.snb.2026.139553","url":null,"abstract":"<div><div>A high-sensitivity seven-channel multipass cell trace gas detection system with optical path length (OPL) of 65.85 m, 44.778 m, 36.876 m, 27.218 m, 24.584 m and two 17.56 m based on TDLAS-WMS technology has been developed, which can simultaneously measure seven different component gases. Wavelength modulation spectroscopy (WMS) was applied to evaluate the system performance with 65.85 m and 44.778 m OPL by measurement of CH<sub>4</sub> and CO<sub>2</sub> at wavelengths of 1653 nm and 2004 nm, respectively. A convolutional neural network (CNN) based regression model was developed for predicting concentrations trends. Kalman filtering method was applied to improve detection sensitivity. Allan deviation analysis demonstrated detection limits (DL) of 3.9 ppb and 14.9 ppb for CH<sub>4</sub> and CO<sub>2</sub> at integration times of 281 s and 903 s, respectively. A 48-hour continuous monitoring atmospheric CH<sub>4</sub> and CO<sub>2</sub> confirmed the system stability and demonstrated suitability for real-time, online detection multiple greenhouse gases.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"454 ","pages":"Article 139553"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fully integrated wearable patch for real-time sweat glucose monitoring toward practical application 面向实际应用的全集成可穿戴式实时汗液血糖监测贴片

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-01 Epub Date: 2026-02-04 DOI: 10.1016/j.snb.2026.139603

Mingpeng Yang , Chengyu Ruan , Jun Cai , Liya Ge , Grzegorz Lisak

{"title":"A fully integrated wearable patch for real-time sweat glucose monitoring toward practical application","authors":"Mingpeng Yang , Chengyu Ruan , Jun Cai , Liya Ge , Grzegorz Lisak","doi":"10.1016/j.snb.2026.139603","DOIUrl":"10.1016/j.snb.2026.139603","url":null,"abstract":"<div><div>Sweat glucose monitoring has emerged as a promising non-invasive approach for real-time tracking of glucose dynamics, with potential applications in diabetes management and exercise-related health analytics. However, the practical deployment of wearable sweat glucose sensors remains challenged by efficient sweat collection, reliable measurement under physiological variability, sustainable power supply, and user comfort. In this study, a fully integrated wearable patch tailored for practical applications was introduced, enabling the real-time collection and measurement of glucose from micro-scale sweat. The patch integrated a paper-based microfluidic chip, an enzymatic electrochemical glucose sensor, and a Near-field communication (NFC)-enabled wireless power and data transmission module into a compact platform (5.6 cm × 3 cm). Glucose analysis can be achieved using only 25 μL of sweat within 28 s, while NFC provided a stable operating voltage of 2.9 V and real-time communication with a smartphone. The glucose sensing electrode was fabricated by electrodepositing a Prussian blue (PB)-doped poly(3,4-ethylenedioxythiophene) (PEDOT) nanocomposite, achieving a sensitivity of 6.9 nA μM<sup>−1</sup> and a linear detection range of 1–1300 μM, with reliable interference resistance and stability. Preliminary on-body tests showed a positive correlation between sweat glucose data from the patch and blood glucose readings from a commercial glucose meter, indicating a similar dynamic trend. By emphasizing system-level integration, battery-free operation, modular architecture, and user-friendly deployment, this work provides an application-oriented wearable platform that facilitates the translation of sweat glucose sensing from laboratory prototypes toward practical continuous monitoring devices for diabetes management and personalized health analytics.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"454 ","pages":"Article 139603"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tetrahedral DNA nanoprobes combined with nano-flow cytometry for single-extracellular vesicle miRNA analysis 四面体DNA纳米探针联合纳米流式细胞术分析单个细胞外囊泡miRNA

IF 3.7 1区化学

Sensors and Actuators B: Chemical Pub Date : 2026-05-01 Epub Date: 2026-02-04 DOI: 10.1016/j.snb.2026.139596

Ping Zhu , Jialing Wang , Yu Li , Jing Jin , Tingting Li , Sha Xu , Ruifang Gao , Wenwen Jia , Sheng Xu , Yunpeng Zhao , Yue Wang

{"title":"Tetrahedral DNA nanoprobes combined with nano-flow cytometry for single-extracellular vesicle miRNA analysis","authors":"Ping Zhu , Jialing Wang , Yu Li , Jing Jin , Tingting Li , Sha Xu , Ruifang Gao , Wenwen Jia , Sheng Xu , Yunpeng Zhao , Yue Wang","doi":"10.1016/j.snb.2026.139596","DOIUrl":"10.1016/j.snb.2026.139596","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) in extracellular vesicles (EVs) play pivotal roles in mediating biological functions and serve as critical quality control markers for cell-derived EV products. This study established an innovative method for target miRNA detection using a Förster resonance energy transfer (FRET)-enabled tetrahedral DNA nanostructure (TDN) probe at the single-EV level. The TDN probe is dual-labeled with Cy3 (donor) and Cy5 (acceptor) fluorophores, engineered to exhibit target-dependent fluorescence switching. MiR-21–5p serves as a characteristic miRNA of mesenchymal stem cell-derived EVs. The platform was applied to simultaneously measure miR-21–5p abundance and EV positive rates. The results show concordance between the fluorescence signal intensity and reverse transcription quantitative polymerase chain reaction (RT-qPCR) measurements, with additional capability for heterogeneity assessment. It maintains high accuracy in serum-mimicking matrices containing physiological interferents, demonstrating interference-resistant performance essential for clinical applications. The platform achieves single-EV resolution while circumventing RT-qPCR amplification bias, with a modular TDN probe design enabling customizable detection of diverse miRNAs. It represents a paradigm shift, advancing EV molecular quantitative detection from bulk-level ambiguity to single-particle-level precision analysis.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"454 ","pages":"Article 139596"},"PeriodicalIF":3.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0