Sensors and Actuators B: Chemical最新文献_第4页

Low-temperature H2S sensor based on in situ growth of hierarchical CuO/NiO microspheres and the sensing mechanism 基于层状CuO/NiO微球原位生长的低温H2S传感器及其传感机理

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-09-29 DOI: 10.1016/j.snb.2025.138893

Hui-Ye Jiang, Wen-Zhi Zhang, Ming Zhao, Ping Wang, Ying-Ming Xu, Hai-Xia Yu, Li-Hua Huo, Li-Li Sui

{"title":"Low-temperature H2S sensor based on in situ growth of hierarchical CuO/NiO microspheres and the sensing mechanism","authors":"Hui-Ye Jiang, Wen-Zhi Zhang, Ming Zhao, Ping Wang, Ying-Ming Xu, Hai-Xia Yu, Li-Hua Huo, Li-Li Sui","doi":"10.1016/j.snb.2025.138893","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138893","url":null,"abstract":"In situ growth of nanomaterials combined with well-defined hierarchical structure may endow a gas sensor with superior sensing capability. Herein, the hierarchical CuO/NiO microspheres were in situ grown on ceramic tubes via a facile one-step ionic liquid (IL)-assisted solvothermal process with subsequent calcination. The resultant CuO/NiO composites demonstrated the interlaced nanosheets-supported microspheres, polycrystalline nature and mesoporous structure. Compared with pristine NiO deposited film sensors, the 4.49 at% CuO modified NiO microspheres sensors exhibited enhanced H2S sensing performances at 92 °C. The response (1157.4) to 100 ppm H2S gas was 7.5 times as high as that of pristine NiO (154.2), and the detection limit was further decreased from 0.1 ppb of the pristine NiO sensor to 0.05 ppb. The dominant H2S sensing mechanism was elaborated from the view of p-p heterojunction, hierarchical architecture as well as the synergistic effect of CuO decorated NiO microspheres. Thus, the in situ deposited sensors based on hierarchical CuO/NiO microspheres presented high selectivity, rapid response and recovery speed, and good stability towards ppm and ppb levels of H2S gas, making it suitable for real-time monitoring of H2S gas in complex environments.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"17 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of a target-stimulated nanoparticle-assembly homogeneous sensing platform for multiplexed DNA methylation assay 用于多重DNA甲基化检测的靶刺激纳米粒子组装均匀传感平台的构建

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-09-29 DOI: 10.1016/j.snb.2025.138891

Feng Dai, Man He, Beibei Chen, Bin Hu

{"title":"Construction of a target-stimulated nanoparticle-assembly homogeneous sensing platform for multiplexed DNA methylation assay","authors":"Feng Dai, Man He, Beibei Chen, Bin Hu","doi":"10.1016/j.snb.2025.138891","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138891","url":null,"abstract":"Detection of multiple tumor-associated DNA methylation variations can shed light on the pathogenesis and physiological characteristics underlying disease development. Herein, we constructed a target-stimulated gold nanoparticles (AuNPs) -assembly homogeneous sensing platform for multiple DNA methylation assays via single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS). The introduction of target methylated DNA could initiate the DNA glycosylase-assisted specific digestion of the Linker probe, thus leading to a reduction in the agglomeration of AuNPs. The AuNP aggregates with varying size distributions can be well discriminated by the intensity and frequency of the Au pulse signal utilizing SP-ICP-MS, thereby enabling dual-mode detection of methylated DNA with good accuracy. Through the modular design of Linker probes, this homogeneous sensing system is feasible for multiplexed DNA methylation analysis, showing good universality. Taking methylated MLH1 and methylated MGMT as representatives, the limit of quantification under the two quantification modes are 25.0 pmol L-1 and 10.0 pmol L-1 for methylated MLH1 and methylated MGMT, respectively. The proposed method was successfully applied to direct analysis of methylated MLH1 and methylated MGMT in 34% human serum samples, demonstrating the robust resistance of the proposed method to biological matrices and its suitability for real biological sample analysis. The programmability and expandability of the homogeneous SP-ICP-MS strategy render it a promising candidate for disease-relevant biomolecule diagnostics and pathogenic mechanism research.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"101 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145188835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Detection of Diphenhydramine in Children’s Cosmetics using SERS-active Gold Hedgehog Particles/MOF-801 deposited on cellulose nanofibrils films 利用sers活性金刺猬颗粒/MOF-801沉积在纤维素纳米原纤维薄膜上快速检测儿童化妆品中的苯海拉明

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-09-29 DOI: 10.1016/j.snb.2025.138883

Meng Jin, Ge Gao, Zeyu Liu, Xiao Meng, Haiyan Wei, Wenwen Chen, Hui Pan, Mengping Zhang, Qiang Jia, Cuijuan Wang

{"title":"Rapid Detection of Diphenhydramine in Children’s Cosmetics using SERS-active Gold Hedgehog Particles/MOF-801 deposited on cellulose nanofibrils films","authors":"Meng Jin, Ge Gao, Zeyu Liu, Xiao Meng, Haiyan Wei, Wenwen Chen, Hui Pan, Mengping Zhang, Qiang Jia, Cuijuan Wang","doi":"10.1016/j.snb.2025.138883","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138883","url":null,"abstract":"Public concern regarding the illicit addition of diphenhydramine (DPH) to children’s cosmetics highlights the urgent need for robust point-of-use (POU) detection strategies. In addressing this concern, we present a surface-enhanced Raman scattering (SERS)-based method for rapidly identifying and quantifying DPH in children’s creams. Our approach utilizes a multifunctional sensor comprising a cellulose nanofibrils (CNFs) film modified with metal-organic framework-801 (MOF-801) and hedgehog-like gold nanoparticles (GHPs). The synthesized GHPs/MOF-801/CNF sensor functions as both a SERS-active substrate and a sorbent, leveraging abundant nanogaps and sharp tips of the GHPs to amplify SERS signals. The porous CNF and MOF-801 ensure efficient and selective extraction, while the SERS-active GHPs significantly enhance detection sensitivity. The correlation between SERS intensity and logarithmic concentration yielded R-values between 0.926 and 0.978, with the detection limit as low as 0.4 ppb, making it suitable for detecting prohibited DPH additives. This method was successfully applied to two brands of cream samples, with results closely aligning with those from routine chromatography. This method’s key advantage is its exceptional efficiency, completing the detection process within 11 min, making it a user-friendly POU assay for detecting prohibited additives in children’s cosmetics.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"70 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial repositioning enables an electrochemical platform for SNP sensing based on a conformationally-switchable hairpin probe 空间重新定位使基于构象可切换发夹探针的SNP传感电化学平台成为可能

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-09-28 DOI: 10.1016/j.snb.2025.138875

Chunyan Liu, Jing Ye, Ximiao Yang, Xiang Tang, Rui Guo, Yuhan Chen, Yunshan Zhang, Jianxian Zeng, Xianzhong Feng, Diming Zhang

{"title":"Spatial repositioning enables an electrochemical platform for SNP sensing based on a conformationally-switchable hairpin probe","authors":"Chunyan Liu, Jing Ye, Ximiao Yang, Xiang Tang, Rui Guo, Yuhan Chen, Yunshan Zhang, Jianxian Zeng, Xianzhong Feng, Diming Zhang","doi":"10.1016/j.snb.2025.138875","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138875","url":null,"abstract":"Accurate identification of single nucleotide polymorphisms (SNPs) holds significant importance for crop genetic improvement and precision breeding. However, traditional detection methods suffer from high costs, operational complexity, and heavy reliance on specialized equipment, limiting their large-scale application in breeding practices. Here, we present an electrochemical biosensing platform based on conformational change design, specifically tailored for sensitive and selective SNP analysis, achieved through the spatial reconfiguration of conformation-switchable hairpin probes. Upon hybridization with target sequences, the hairpin structure undergoes spatial remodeling to modulate its distance from the electrode surface, enabling signal transduction. This design leverages precise spatial control and structural dynamics to achieve high signal specificity, allowing single-base mismatch discrimination with exceptional reproducibility and sensitivity. The operationally simple biosensing platform demonstrates high specificity in distinguishing single-nucleotide variations. By applying this system to identify SNPs associated with key phenotypic traits related to leaf morphology in soybean genotypes, we validate its potential for genotype screening and molecular breeding applications.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"36 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Dual-Responsive NIR Fluorescent Probe for Diagnosis and Therapeutic Evaluation of Alcoholic Liver Disease and Drug-Induced Liver Disease 双响应NIR荧光探针对酒精性肝病和药物性肝病的诊断和治疗评价

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-09-28 DOI: 10.1016/j.snb.2025.138873

Siyu Gao, Shuo Wang, Mei Li, Wei Zhang, Wenjing Zhang, Lei Yang, Zhanxian Li, Mingming Yu

{"title":"A Dual-Responsive NIR Fluorescent Probe for Diagnosis and Therapeutic Evaluation of Alcoholic Liver Disease and Drug-Induced Liver Disease","authors":"Siyu Gao, Shuo Wang, Mei Li, Wei Zhang, Wenjing Zhang, Lei Yang, Zhanxian Li, Mingming Yu","doi":"10.1016/j.snb.2025.138873","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138873","url":null,"abstract":"Acute liver injury (ALI), including alcoholic liver disease (ALD) and drug-induced liver injury (DILI), poses significant diagnostic and therapeutic challenges because of the absence of specific biomarkers that distinguish it from other pathologies (e.g., acute lung or skeletal muscle injuries). Here, we report the synthesis of a probe, TXET, which exhibits dual responsiveness to viscosity and sulfur dioxide (SO2) derivatives. The probe’s carbon-carbon double bond undergoes a Michael addition reaction with bisulfite ion (HSO3−), blocking the photoinduced electron transfer (PET) process and enabling “off-on” detection of SO2 derivatives. Concurrently, as viscosity increases, rotation of the carbon-carbon bond in TXET is restricted, leading to the emission of intense red fluorescence. In vivo studies demonstrated that TXET can monitor dynamic changes in SO2 concentration and viscosity in ALD and DILI mice. Furthermore, TXET facilitated a comparative assessment of four drugs used in ALI treatment, revealing distinct therapeutic effects of these drugs in ALD and DILI models. This work highlights that TXET serves as a promising tool for the diagnosis of ALI and evaluation of therapeutic efficacy.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"18 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas12a-Triggered DNA Windmill-Driven Dual-Mode Self-Powered Biosensor with Capacitor Signal Amplification for Ultrasensitive and Portable Detection of Thalassemia 带有电容信号放大的CRISPR/ cas12a触发DNA风车驱动双模式自供电生物传感器用于超灵敏和便携式检测地中海贫血

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-09-28 DOI: 10.1016/j.snb.2025.138876

Ya-Qian Xie, Shi-Yu Liu, Ya-Shu Wei, Ruo-Ling Ya, Yan-Ni Luo, Xue-Cai Tan, Ke-Jing Huang, De-Fen Feng

{"title":"CRISPR/Cas12a-Triggered DNA Windmill-Driven Dual-Mode Self-Powered Biosensor with Capacitor Signal Amplification for Ultrasensitive and Portable Detection of Thalassemia","authors":"Ya-Qian Xie, Shi-Yu Liu, Ya-Shu Wei, Ruo-Ling Ya, Yan-Ni Luo, Xue-Cai Tan, Ke-Jing Huang, De-Fen Feng","doi":"10.1016/j.snb.2025.138876","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138876","url":null,"abstract":"Herein, we report a CRISPR/Cas12a-triggered DNA windmill-driven dual-mode self-powered biosensor integrated with capacitor signal amplification for the ultrasensitive and portable detection of the thalassemia gene CD17. This innovative platform combines molecular recognition, nanoengineering, and portable electronics to overcome limitations in conventional thalassemia diagnosis. The DNA windmill nanostructure (DW), self-assembled from four DNA strands, provides abundant methylene blue (MB) binding sites for dual-signal amplification. CRISPR/Cas12a acts as a programmable molecular switch, activating trans-cleavage upon recognizing CD17-triggered RCA products, precisely regulating DW integrity and MB dissociation. The AuNPs/graphdiyne nanocomposite-modified bioelectrode significantly enhances electron transfer efficiency, achieving great current amplification through sp/sp2 hybrid carbon networks and plasmonic effects. By coupling enzyme-powered biofuel cells with a 1000 μF capacitor, the system converts biochemical signals into amplified electrical outputs while enabling Bluetooth-enabled smartphone readouts for field deployment. The dual-mode detection, integrating electrochemical and colorimetric channels, demonstrates ultra-low detection limits of 36.0 aM (electrochemical) and 52.1 aM (colorimetric) with a 5-log dynamic range (0.0001–10,000 pM). Clinical validation shows 96.5-108.6% recovery in human serum, supported by <5% RSD in reproducibility tests. This work pioneers CRISPR-powered portable diagnostics with CRISPR-windmill signal transduction and capacitor-boosted sensitivity, offering transformative potential for point-of-care genetic disease screening.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"18 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interface Engineering of MXene using ZSM-5 Molecular Sieve: Atomic-Level Charge Transfer Pathways for Ultra-Sensitive NO2 Detection ZSM-5分子筛MXene界面工程：超灵敏NO2检测的原子级电荷转移途径

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-09-28 DOI: 10.1016/j.snb.2025.138870

Shenghui Xia, Chen Chen, Jinhong Liu, Yu Zhang, Hui Zhang, Thomas Thundat, Ajit Khosla

{"title":"Interface Engineering of MXene using ZSM-5 Molecular Sieve: Atomic-Level Charge Transfer Pathways for Ultra-Sensitive NO2 Detection","authors":"Shenghui Xia, Chen Chen, Jinhong Liu, Yu Zhang, Hui Zhang, Thomas Thundat, Ajit Khosla","doi":"10.1016/j.snb.2025.138870","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138870","url":null,"abstract":"Nitrogen dioxide (NO2) is a highly toxic air pollutant that endangers human health, environmental sustainability, and ecosystems. To address this issue, we designed an inorganic heterogeneous interface by integrating ZSM-5 molecular sieves with MXene via a facile synthesis route. This unique architecture synergizes the dual cross-channel framework of ZSM-5 and the high electrical conductivity and abundant surface functional groups of MXene. This combination greatly enhances electron transfer kinetics, interfacial NO2 adsorption, and gas sensing properties. The optimized MXene/ZSM-5 nanocomposite achieves an ultra-low NO2 detection limit of 1 ppm. Its response is 79.78% higher than that of original MXene. The nanocomposite also exhibits excellent stability and significant anti-interference properties. Correlation studies confirm consistent performance across humidity and atmospheric conditions. Density functional theory (DFT) calculations elucidated the charge transfer pathways and adsorption mechanisms at the atomic level that enhance sensitivity. By pioneering the MXene/ZSM-5 interface and systematically investigating its gas interaction kinetics, this work provides fundamental insights for designing next-generation MXene-based gas sensors.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"1 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-metal nanozyme loaded (Zr/Ce) UiO-66 driven self-cascade catalysis nanoreactor based on split aptamer for sensitive detection of pathogenic bacteria 基于分裂适体的多金属纳米酶负载（Zr/Ce） UiO-66驱动自级联催化纳米反应器用于病原菌的灵敏检测

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-09-28 DOI: 10.1016/j.snb.2025.138871

Yang Song, Guoyang Xie, Luyang Zhao, Yi Zhao, Hengyi Xu

{"title":"Multi-metal nanozyme loaded (Zr/Ce) UiO-66 driven self-cascade catalysis nanoreactor based on split aptamer for sensitive detection of pathogenic bacteria","authors":"Yang Song, Guoyang Xie, Luyang Zhao, Yi Zhao, Hengyi Xu","doi":"10.1016/j.snb.2025.138871","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138871","url":null,"abstract":"Pathogenic bacteria pose a significant impact on human and environmental health. Therefore, establishing a rapid and sensitive method for detecting these bacteria is urgent. In this study, a self-cascade catalysis nanoreactor based on split aptamers (SPA) was developed for the sensitive detection of E. coli O157:H7, enabling a self-powered H2O2 strategy and improved convenience. This approach employed (Zr/Ce) UiO-66 anchored with an AuRhPt trimetallic nanozyme. The incorporation of Ce enhances the specific surface area and catalytic activity of Zr UiO-66. The AuRhPt@(Zr/Ce) UiO-66 nanozyme exhibits multiple catalytic activities, such as glucose oxidase (GOx) and peroxidase (POD) activities, enabling the development of a self-powered H2O2 strategy. Subsequently, the aptamer was split into two shorter fragments to minimize steric hindrance, reduce costs, and achieve high performance. A self-cascade catalysis nanoreactor was developed using a split aptamer recognition and assembly strategy for the rapid and sensitive detection of E. coli O157:H7 in different real samples. The linear range for E. coli O157:H7 was 101–105 CFU/mL in lake water, lettuce, and milk samples, with a limit of detection (LOD) of 101 CFU/mL and recoveries ranging from 81% to 117%. Consequently, this self-cascade catalysis nanoreactor is well-suited to the sophisticated detection requirements of diverse samples and shows significant promise as a highly sensitive and efficient approach for identifying other targets.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"4 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Portable histone assay technology (PHAST) for the sample-to-answer detection of circulating histones in whole blood 便携式组蛋白测定技术（PHAST）用于全血中循环组蛋白的样本到应答检测

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-09-27 DOI: 10.1016/j.snb.2025.138848

Rachel L. Warren, John R. Rzasa, Zehra F. Syeda, Alexander R. Pueschel, Ian M. White

{"title":"Portable histone assay technology (PHAST) for the sample-to-answer detection of circulating histones in whole blood","authors":"Rachel L. Warren, John R. Rzasa, Zehra F. Syeda, Alexander R. Pueschel, Ian M. White","doi":"10.1016/j.snb.2025.138848","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138848","url":null,"abstract":"Circulating histones are a potential biomarker for organ failure, organ transplant rejection, and sepsis. There is a need for point-of-care detection of histones in patient samples to enable diagnostics in the emergency department (sepsis), military field hospitals (trauma-induced organ failure), and at home (transplant rejection). For these applications, it is necessary to detect histones directly from whole blood samples without any intermediate steps and with low-cost portable instrumentation. Here we introduce portable histone assay technology (PHAST), an assay and a low-cost handheld instrument for the sample-to-answer detection of histones directly from blood samples. The assay utilizes a DNA-based FRET reporter molecule to enable direct detection of histones in a single step, while anti-red-blood-cell magnetic beads in combination with a moveable magnet in the handheld instrument remove the red blood cells prior to the fluorescence measurement. Using this novel approach, we detect clinically relevant concentrations of histones spiked into whole blood in under 20 minutes.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"59 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Point-of-care testing of hyaluronidase in the urine using commercial pregnancy test strips as readout 尿中透明质酸酶的即时检测使用商业妊娠试纸作为读数

IF 8.4 1区化学

Sensors and Actuators B: Chemical Pub Date : 2025-09-27 DOI: 10.1016/j.snb.2025.138851

Jing Yang, Zhonghui Chen, Yue Lin, Cuiying Lin, Fang Luo, Bin Qiu, Jian Wang, Zhenyu Lin, Huixing Chen

{"title":"Point-of-care testing of hyaluronidase in the urine using commercial pregnancy test strips as readout","authors":"Jing Yang, Zhonghui Chen, Yue Lin, Cuiying Lin, Fang Luo, Bin Qiu, Jian Wang, Zhenyu Lin, Huixing Chen","doi":"10.1016/j.snb.2025.138851","DOIUrl":"https://doi.org/10.1016/j.snb.2025.138851","url":null,"abstract":"Elevated hyaluronidase (HAase) concentration has been found in the urine samples of the patients with bladder cancer and development of simple but sensitive detection methods for HAase is important for related disease screening. Pregnancy test strips (PTSs) is one of the most common portable and inexpensive household testing strips, which has been used to measure human chorionic gonadotropin (HCG) in urine sample for pregnancy test. In this study, a portable biosensor has been developed for HAase using PTSs as detection device. HCG was embedded in a hydrogel format through the cross-linking of hyaluronic acid (HA) and polyethylenimine (PEI). The presence of HAase can digest HA in the hydrogel and results in the releasing of HCG, which can be detected by a PTS easily. The color changing of the test line (T line) indicates the existence of the target object and the depth of color can be used for semi-quantitative detection of the target concentration. The ImageJ software was used to extract the RGB value of the color of T line. The different of the RGB value (ΔRGB) at present and absent of target has a strong linear correlation with the HAase concentration between 1 U/mL and 100 U/mL, with a limit of detectionof 0.27 U/mL (S/N = 3). The proposed method was used to detect HAase in urine samples from non-pregnancy healthy individuals and patients diagnosed with bladder cancer as a demonstration.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"61 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0