Sensors and Actuators B: Chemical最新文献

{"title":"Crosstalk-free two-channel visualization and precise assessment of autophagy-regulated hepatic ischemia-reperfusion injury by a two-photon fluorescent probe","authors":"Qing Chang, Qi Wang, Jingyu Zhang, Yan Fang, Jiale Ou, Huimin Zhou, Xianyun Xu, Qi Chen, Yan Feng, Xiangming Meng","doi":"10.1016/j.snb.2025.137402","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137402","url":null,"abstract":"Hepatic ischemia-reperfusion injury (HIRI) is a universal surgical complication that seriously affects patient prognosis. Many studies have shown that its process is closely associated with cellular autophagy, and the inhibition of autophagy can slow down HIRI. Autophagy is intensely related to changes in the lysosomal microenvironment. Therefore, real-time fluorescence visualization of autophagy level fluctuations through monitoring lysosomal microenvironment could be a viable strategy for effectively assessing HIRI. In our work, we reported a two-photon fluorescent probe <strong>Ly-NJ</strong>, which responds to changes in lysosomal polarity and viscosity in two crosstalk-free channels. The correlation between lysosomal polarity/viscosity during HIRI and autophagy levels was found. The results of two-photon fluorescence imaging as well as Western blot revealed that the increase in lysosomal polarity and viscosity during prolonged hepatic ischemia-reperfusion corresponded to the increased levels of autophagy. In addition, two-channel imaging results showed that the use of autophagy inhibitor 3-methyladenine (3-MA) in HIRI can recover the lysosomal polarity and viscosity to the initial level of control group, indicating HIRI can be alleviated by inhibiting the autophagy level. Furthermore, luteolin and silymarin were testified to have a mitigating effect on HIRI by monitoring the variation of blue and red channels, which was similar with that of 3-MA. <em>In vivo</em> fluorescence imaging in HIRI model mice further supported the retarding effects of above agents on HIRI. These studies indicated that <strong>Ly-NJ</strong> has the potential for clinical application in visualizing HIRI, and will provide a promising imaging tool for timely prophylactic treatment and intraoperative monitoring of HIRI.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"57 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UiO-66@TiO2/Au heterojunction-like SERS substrate based on adsorption-induced aggregation for detection of thiamethoxam and triazophos in apple juice","authors":"Shengqiao Gao, Qingyi Wei, Hongbin Pu","doi":"10.1016/j.snb.2025.137398","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137398","url":null,"abstract":"Heterojunction-like substrates have drawn significant attention in surface enhanced Raman spectroscopy (SERS) owing to their multi-enhancement characteristics. In this study, the core-shell architectures of UiO-66@ TiO₂ were fabricated for capture and enrichment of organophosphorus pesticides (OPs) in solution. After absorption of OPs, the SERS-active nanoparticles of Au were assembled on the core-shell architectures to form UiO-66@TiO₂/Au substrate via Au-S bonding between OPs molecule and AuNPs and electrostatic interactions between UiO-66@TiO₂ and Au, resulting in adsorption-induced aggregation of Raman signal for OPs. The proposed method of adsorption-induced aggregation could be used for detecting thiamethoxam and triazophos in apple juice with detection limit of 3.51<!-- --> <!-- -->μg/L and 0.66<!-- --> <!-- -->μg/L, respectively. Furthermore, the satisfactory recovery rates, ranging from 91.98% to 104.31% for thiamethoxam and from 91.47% to 116.47% for triazophos, indicated that the prepared heterojunction-like substrate could be potentially used for trace detecting OPs in foods.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"79 2 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bifunctional strategy based on MoS2/N,S-CQDs/Bi2S3 nanocomposite photosensitive materials: Ultrasensitive detection of prostate antigens and preliminary design of a portable detector","authors":"Yongxue Li ,&nbsp;Runuo Wang ,&nbsp;Chunling Mao ,&nbsp;Qian Hang ,&nbsp;Longshan Zhao","doi":"10.1016/j.snb.2025.137305","DOIUrl":"10.1016/j.snb.2025.137305","url":null,"abstract":"<div><div>In this work, a novel photoelectrochemical (PEC) immunosensor based on molybdenum disulfide (MoS₂)/nitrogen and sulfur co-doped carbon quantum dots (N,S-CQDs)/bismuth sulfide (Bi₂S₃) nanocomposites was designed for the quantitative detection of prostate-specific antigen (PSA) in human serum and urine. For the initial time, we used the synthesized N,S-CQDs as a Mo⁴⁺ and Bi³⁺ sulfur source. A one-step hydrothermal process was used to create highly photoactive MoS₂/N,S-CQDs/Bi₂S₃ nanoheterojunctions. Compared to pure MoS₂ and Bi₂S₃, MoS₂/N,S-CQDs/Bi₂S₃ exhibited better photoelectric activity and electron transfer efficiency. By the layer-by-layer assembly method, the specificity between PSA and prostate-specific antibody (Anti-PSA) was used to form an immune complex, which increased the spatial site resistance of MoS₂/N,S-CQDs/Bi₂S₃, leading to a decrease in photocurrent transmission efficiency, thus reaching the purpose of direct detection of PSA concentration. Under the optimized conditions, the photoelectric signal intensity of the MoS₂/N,S-CQDs/Bi₂S₃ PEC immunosensor showed a good linear relationship with the concentration of PSA in the range of 0.001 ng/mL to 50 ng/mL, and the limit of detection (LOD) was 0.35 pg/mL. Regarding quantitative PSA detection, the developed PEC immunosensor offers outstanding selectivity, sensitivity, and stability, which can provide an effective strategy for diagnosing and treating prostate cancer. In addition, to detect PSA and other biomarkers with speed and precision, a novel portable PEC detector model was proposed and designed in this study, and the construction of this model has great potential for application in clinical and other disease diagnosis.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137305"},"PeriodicalIF":8.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143124678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An intelligent sensing array for thermal runaway characteristic gas concentration prediction based on SACNN-Mamba","authors":"Meng Tang, Xin Zhang, Chang Zhang, Tongbin Chen, Xinxin Yan, Jie Zou, Wanlei Gao, Qinghui Jin, Jiawen Jian","doi":"10.1016/j.snb.2025.137368","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137368","url":null,"abstract":"To address the issue of cross-sensitivity when using gas signals for early warning of thermal runaway, an efficient algorithm based on the intelligent sensing array is proposed. The algorithmic model introduced in this paper is a fusion model that incorporates a feature self-attention module, a 1DCNN, and a Mamba module, aiming to enhance the regression prediction accuracy of the intelligent sensing array for gas mixture concentrations. The study demonstrates the effectiveness of our fusion network model in accurately predicting the concentrations of various target gases (such as H₂, CO, and C₂H₄) in gas mixtures. The coefficients of determination (R²) were 99.74%, 99.45%, and 99.48% respectively, indicating that the model fits the sensor data very well and can predict changes in the data with high accuracy. The Root Mean Square Errors (RMSE) were 2.3514, 4.1752, and 3.7164, respectively. The Mean Absolute Errors (MAE) were 1.4398, 2.3846, and 2.5088, respectively. Additionally, the Symmetric Mean Absolute Percentage Errors (SMAPE) were 2.1212, 2.6272, and 2.6515. These values indicate that the model has high predictive accuracy and demonstrates good generality and robustness. The method proposed in this work holds significant potential for application in the field of gas warning for thermal runaway in lithium batteries.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"12 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrophobicity-promoted humidity-resistant ethanol sensors based on PTFE/Au/WO3 composite films","authors":"Xiaojie Zhu ,&nbsp;Xueting Chang ,&nbsp;Yingchang Jiang ,&nbsp;Weixiang Gao ,&nbsp;Shibin Sun","doi":"10.1016/j.snb.2025.137386","DOIUrl":"10.1016/j.snb.2025.137386","url":null,"abstract":"<div><div>The ability of hydrophobic surfaces to repel water make them attractive for preparing humidity-resistant gas-sensing films. Herein, we deposited the Au nanoparticles and the polytetrafluoroethylene (PTFE) film onto the WO₃ film sequentially to form the PTFE/Au/WO₃ composite films by using methods of ion beam sputtering and radio-frequency (RF) magnetron sputtering. The PTFE/Au/WO₃ composite films-based gas sensors exhibited high sensitivity towards ethanol with fast response/recovery rate, wide detection range, good selectivity, and high stability. Due to the hydrophobic feature of the PTFE film, the PTFE/Au/WO₃ composite films-based gas sensors demonstrated promising humidity-tolerant properties, which achieved a high response retention of as high as 88.7 % as the relative humidity (RH) increased from 30 % to 90 %. This work provides a new strategy for the development of metal oxide semiconductor (MOS)-based gas sensors that can work effectively under high humidity.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137386"},"PeriodicalIF":8.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrical and fluorescent sensing-based dual-functional detection strategy for ultrasensitive antibiotics analysis","authors":"Xiaojie Wei, Tian Tao, Ziwei Ye, Qiuju Li, Zhuo Li, Shun Mao","doi":"10.1016/j.snb.2025.137384","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137384","url":null,"abstract":"Kanamycin (KAN), a typical aminoglycoside antibiotic, has been frequently found in the environment, which causes threats to human health and ecosystems. Ultrasensitive and reliable antibiotic detection strategies are urgently needed. In this study, we propose a dual-functional MXene-based sensing strategy for antibiotic analysis. The sensing platform is fabricated by extended-gate field-effect transistor (EG-FET) with a commercial MOSFET and an extended gate as the sensing electrode. Through electrostatic interaction, Ti₃C₂T_x MXene nanosheets are assembled on ITO glass gate and double-stranded DNA (dsDNA) is modified on MXene surface. The dsDNA is composed of ssDNA and its complementary strand (cs-DNA). In particular, the ssDNA is the specific recognition element for KAN. KAN can compete with csDNA and disrupt the base pairing of dsDNA, causing the release of csDNA. Relying on the EG-FET sensing structure, a fluorescence detection strategy is also developed based on the quenching process of fluorophore (6-Carboxyfluorescein, 6-FAM) labeled cs-DNA (6-FAM-csDNA) on Ti₃C₂T_x MXene, in which the fluorescence intensity of 6-FAM is used as the signal for detecting KAN. This dual-functional MXene-based sensor offers both current response and fluorescence response signals in KAN detection. The reported sensor achieves an ultrasensitive detection performance for KAN with a detection limit of 6.44 fM. The sensor's ability to detect KAN in real water samples further demonstrates its practical application potential in complex environment. This work provides a novel dual-functional strategy for sensitive and highly specific detection of antibiotics, addressing some of the key obstacles in antibiotics detection in various applications.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"59 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive and Reversible Sensing of Oxygen Based on Transition Metals Doped Quasi-2D Layered Perovskites with 4T1→6A1 Luminescence","authors":"Fangyuan Lin, Xuelian Liu, Min Ye, Jingwen Jin, Xi Chen","doi":"10.1016/j.snb.2025.137382","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137382","url":null,"abstract":"Lead halide perovskites (LHPs) with rich optical properties have shown good prospects in sensing applications through rational design of their composition and structure. Although luminescent LHPs have been proposed as emerging probes for oxygen gas, their various oxygen-sensitive optical properties are still providing new ideas for oxygen sensing research, and oxygen sensing performance of these materials also needs to be improved. Here, a new class of metal-doped LHPs with quasi-two-dimensional (2D) layered structure and ⁴T₁→⁶A₁ luminescence has been fabricated, and their oxygen sensing capability has been demonstrated. As a representative, the layered perovskite A₂CsPb₂Cl₇ with a wealth of accessible Mn²⁺ dopants exhibits a sensitive response to the change of oxygen contents (<em>I</em>_<em>0</em>/<em>I</em>_<em>100</em> = 9.75). By analyzing the effect of molecular oxygen on this material during sensing, we highlight that oxygen sensing responses of Mn²⁺-doped quasi-2D LHP are derived from the excited state of ⁴T₁→⁶A₁ luminescence. Dynamic deactivation of Mn²⁺-d excited state by O₂ molecules results in rapid quenching of Mn²⁺ emission and a reversible sensing phenomenon. In addition, Fe³⁺ dopants with the same ⁴T₁→⁶A₁ luminescence are introduced into the quasi-2D layered perovskite, so the possible mechanism has been further supported by similar oxygen sensing behaviors of Fe³⁺ emission. In our perception, above results make these perovskites candidates for oxygen sensing materials, for example, linear response was achieved at normal oxygen levels from 0% to 21%. Therefore, this work shows a new oxygen sensing platform based on the quasi-2D layered LHPs with ⁴T₁→⁶A₁ luminescence.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"132 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Line excitation SERS spectroscopy using laser-induced graphene coated with silver nanoparticles","authors":"Jiajun Li,&nbsp;Yunyun Mu,&nbsp;Guanwei Tao,&nbsp;Xinping Zhang","doi":"10.1016/j.snb.2025.137375","DOIUrl":"10.1016/j.snb.2025.137375","url":null,"abstract":"<div><div>We report a line excitation scheme for surface enhanced Raman scattering (SERS) using a graphene substrate deposited with silver nanoparticles (AgNPs). Direct laser writing was employed for producing laser-induced graphene (LIG) layer on polyimide and electrochemical reaction was used to grow AgNPs onto the surface of LIG. In addition to the local-field enhancement on the plasmonic hotspots, the graphene-based charge-transfer and molecular adsorption properties make further contributions to the SERS mechanisms. Compared with conventional point excitation, the line excitation scheme allows much extended dynamic range of the excitation laser power before reaching the damage threshold of the target molecules, much enhanced Raman signal due to the integration over the focusing line, and high reliability/repeatability of the SERS signals. This also reduces significantly the sensitivity of the SERS signals to the randomly distributed nanostructures. Thus, much enlarged excitation area/volume for Raman interactions enables high stability of the signals with multiplied intensity through integration over the focusing line, as compared with point excitation scheme under the same excitation power density. The line excitation scheme shows a detection limit of 100 ppb in tracing melamine in water, attaining an enhancement factor of larger than 100, as compared with point excitation.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137375"},"PeriodicalIF":8.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of multimeric peptide-MHC with SpyCatcher modules for antigen-specific T cell detection","authors":"Xiangyao Wang ,&nbsp;Mengyu Zhang ,&nbsp;Enli Zhang ,&nbsp;Qiwei Wang ,&nbsp;Yiqing Guo ,&nbsp;Dingfan Xu ,&nbsp;Congran Yue ,&nbsp;Haozhe Cui ,&nbsp;Zhiming Zhao ,&nbsp;Sheng Ye ,&nbsp;Si Liu","doi":"10.1016/j.snb.2025.137342","DOIUrl":"10.1016/j.snb.2025.137342","url":null,"abstract":"<div><div>Peptide-major histocompatibility complex (pMHC) multimers, most commonly used in streptavidin-based tetrameric form, are exceptional for detecting, isolating, and analyzing antigen-specific T cells. However, classical pMHC tetramer staining methods may not detect fully functional T-cells with lower-affinity T-cell receptors (TCRs), which are common in antitumor and autoimmune responses. This limitation has driven the innovation of new methods to improve pMHC binding through multivalent scaffolds, thereby increasing the avidity of weak TCR-pMHC interactions. In this study, we present a novel method for creating multimeric pMHC complexes using the SpyCatcher/SpyTag system. Our approach simplifies the multimer production process by eliminating biotinylation steps, instead employing SpyTag technology for efficient assembly. The method allows for the production of MHC molecules with SpyTags, which can be coupled to various SpyCatcher variants, enabling diverse oligomeric configurations. Our research demonstrates that these novel multimeric pMHC complexes significantly enhance the binding avidity for low-affinity antigen-specific TCRs compared to classical tetramers. This advancement presents a versatile platform for T cell response studies across diverse immunological contexts, offering advantages in specificity, stability, and scalability for both basic research and clinical applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137342"},"PeriodicalIF":8.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seeing is Believing: Facile Amphiphilic AIEgen Unveils Selective and Ultrafast Wash-Free Dead Cell Imaging","authors":"Kaixin Yang ,&nbsp;Yuxin Yang ,&nbsp;Leilei Si ,&nbsp;Weihan Guo ,&nbsp;Guosheng Zhang ,&nbsp;Guomin Xia ,&nbsp;Hongming Wang","doi":"10.1016/j.snb.2025.137383","DOIUrl":"10.1016/j.snb.2025.137383","url":null,"abstract":"<div><div>The differentiation between live and dead cells is essential for both pharmacological and pathological research. However, current fluorescence probes face challenges due to their cumbersome methodologies and a lack of real-time imaging capabilities. In this study, we introduce an amphiphilic aggregation-induced emission luminogen (AIEgen) that incorporates two ammonium salts with the AIE building block of squaraine dyes. The resulting compound, SQ-N, exhibits negligible emission in aqueous and organic solvents as well as in the solid state, while displaying vivid orange fluorescence specifically in glycerol. This AIEgen has demonstrated the ability to selectively ‘light up’ dead cells with rapid, wash-free kinetics within seconds at room temperature, thereby presenting an ultrafast and user-friendly staining protocol. Additionally, the superior biocompatibility, long-term retention, and photostability of SQ-N render it more suitable for real-time and in situ monitoring of cell viability, thus serving as an effective alternative to commercial probes. Our work offers new insights into the amphiphilic AIEgen and its potential for the development of cellular imaging probes.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137383"},"PeriodicalIF":8.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}