Xueying Gao, Luning Wang, Yi Chen, Xi Ma, Minyi Hui, Zhouping Wang, Xiaoyuan Ma
{"title":"A Facile Aptasensor Based on Au Dimers and Carbon Dots for SERS/Fluorescence Dual-Mode Detection of AFB1","authors":"Xueying Gao, Luning Wang, Yi Chen, Xi Ma, Minyi Hui, Zhouping Wang, Xiaoyuan Ma","doi":"10.1016/j.snb.2025.138297","DOIUrl":null,"url":null,"abstract":"A dual-signal aptasensor that integrates Surface-Enhanced Raman Scattering (SERS) and fluorescence has been developed for the sensitive detection of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>). 2,2'-bipyridine ethylene (BPE) linked to gold nanoparticles (AuNPs) through \"Au-N\" bonds formed Au dimers with nanogaps. These were functionalized with complementary DNA (cDNA) of the AFB<sub>1</sub> apt, serving as both a Raman signal probe and a fluorescence quencher. The Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>-carbon dots (CDs), modified with the AFB<sub>1</sub> apt, functioned as a fluorescent magnetic substrate facilitating efficient separation. The components assembled into a satellite-like structure, which maximized SERS signals while simultaneously quenching fluorescence. Upon the addition of AFB<sub>1</sub>, the Raman probe was released, leading to a reduction in SERS signals and recovery of fluorescence. The sensor demonstrated an extensive linear detection range from 10⁻⁴ to 10² ng/mL, accompanied by an ultra-low limit of detection (LOD) of 0.066<!-- --> <!-- -->pg/kg. Spiked recovery rates in peanut, rice, and corn samples ranged from 100.35% to 109.75%, indicating high accuracy and applicability for AFB<sub>1</sub> detection in real-world samples.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"3 1","pages":""},"PeriodicalIF":8.0000,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors and Actuators B: Chemical","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.snb.2025.138297","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
A dual-signal aptasensor that integrates Surface-Enhanced Raman Scattering (SERS) and fluorescence has been developed for the sensitive detection of aflatoxin B1 (AFB1). 2,2'-bipyridine ethylene (BPE) linked to gold nanoparticles (AuNPs) through "Au-N" bonds formed Au dimers with nanogaps. These were functionalized with complementary DNA (cDNA) of the AFB1 apt, serving as both a Raman signal probe and a fluorescence quencher. The Fe3O4@SiO2-carbon dots (CDs), modified with the AFB1 apt, functioned as a fluorescent magnetic substrate facilitating efficient separation. The components assembled into a satellite-like structure, which maximized SERS signals while simultaneously quenching fluorescence. Upon the addition of AFB1, the Raman probe was released, leading to a reduction in SERS signals and recovery of fluorescence. The sensor demonstrated an extensive linear detection range from 10⁻⁴ to 10² ng/mL, accompanied by an ultra-low limit of detection (LOD) of 0.066 pg/kg. Spiked recovery rates in peanut, rice, and corn samples ranged from 100.35% to 109.75%, indicating high accuracy and applicability for AFB1 detection in real-world samples.
期刊介绍:
Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.