Photoluminescence Switches with Ultralarge Stokes Shift for Cellular Membrane Lipid Order Imaging

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-07-09 DOI:10.1016/j.snb.2025.138299

Hongbei Wei, Hui Zhang, Ke Wei, Liren Xu, Xiangkun Si, Xiaolong Sun

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Abstract

Lipid order in cellular membranes plays a critical role in cellular functions and is implicated in various pathological conditions. Reliable detection tools are essential for elucidating the biological mechanisms of diseases initiated by lipid order alterations and for monitoring disease progression. Herein, we report a solvatochromic probe with an ultralarge Stokes shift of approximately 200 nm based on indanonealkene luminescent platform, for distinguishing plasma membrane lipid order. Theoretical calculations corroborated the probe’s twisted intramolecular charge transfer properties, and cellular experiments validated its cell membrane targeting and lipid order discrimination capabilities, further confirmed in giant unilamellar vesicles models. Furthermore, the probe TPA-CA-3 allows the detection of dynamic lipid order variations in live cells under mechanical force, cholesterol depletion, and cholesterol enrichment. We anticipate that this large Stokes shift fluorescence probe will serve as a valuable tool for tracking and quantifying lipid order across diverse biological processes.

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具有超大斯托克斯位移的光致发光开关用于细胞膜脂质阶成像

细胞膜中的脂质顺序在细胞功能中起着关键作用，并与各种病理状况有关。可靠的检测工具对于阐明由脂质顺序改变引发的疾病的生物学机制和监测疾病进展至关重要。在此，我们报道了一种基于吲哚酮发光平台的超大斯托克斯位移的溶剂变色探针，用于区分质膜脂质顺序。理论计算证实了探针的分子内扭曲电荷转移特性，细胞实验证实了其细胞膜靶向和脂质顺序识别能力，并在巨大的单层囊泡模型中进一步证实。此外，探针TPA-CA-3可以检测活细胞在机械力、胆固醇消耗和胆固醇富集作用下的动态脂质顺序变化。我们预计这种大型Stokes位移荧光探针将作为一种有价值的工具，用于跟踪和量化不同生物过程中的脂质顺序。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.