Ultrasonics Sonochemistry最新文献

{"title":"High-frequency ultrasound induced the preparation of oxidized low density lipoprotein","authors":"Yuanmin Li ,&nbsp;Wanyue Yang ,&nbsp;Xinyi Zhang ,&nbsp;Jingjing Ba ,&nbsp;Han Yang ,&nbsp;Wen Wang ,&nbsp;Ke Zhang ,&nbsp;Ze Yang ,&nbsp;Hui Liang ,&nbsp;Zihan Li ,&nbsp;Muthupandian Ashokkumar ,&nbsp;Jiguo Zhang ,&nbsp;Zhiliang Gao ,&nbsp;Yang Yu","doi":"10.1016/j.ultsonch.2025.107303","DOIUrl":"10.1016/j.ultsonch.2025.107303","url":null,"abstract":"<div><div>Foam cells have been frequently used in studies related to atherosclerosis. Traditional methods for inducing oxidized low-density lipoprotein (oxLDL) involve copper ion (Cu²⁺) treatment, which has inherent limitations such as prolonged oxidation times and residual copper ions. This study explored high-frequency ultrasound (400 kHz) as an alternative method for LDL oxidization. The findings demonstrated that high-frequency ultrasound-oxidized LDL (U-oxLDL) exhibited no significant differences compared to copper-oxidized LDL (Cu-oxLDL) in terms of electrophoretic mobility, foam cell morphology, lipid content, and cholesterol transport proteins. Additionally, lipidomic analysis revealed that U-oxLDL was more comparable to native LDL (N-LDL). Transcriptomic profiling of bone marrow-derived macrophages (BMDMs) treated with oxLDL showed that the gene expression patterns of BMDM foam cells treated with U-oxLDL were over 90 % consistent with those treated with Cu-oxLDL. Therefore, high-frequency ultrasound oxidation method represents a green and efficient strategy for oxLDL preparation, offering potential advantages for advancing atherosclerosis research.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"115 ","pages":"Article 107303"},"PeriodicalIF":8.7,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable extraction of phytochemicals from Mentha arvensis using supramolecular eutectic solvent via microwave Irradiation: Unveiling insights with CatBoost-Driven feature analysis","authors":"Zubera Naseem ,&nbsp;Muhammad Bilal Qadir ,&nbsp;Abdulaziz Bentalib ,&nbsp;Zubair Khaliq ,&nbsp;Muhammad Zahid ,&nbsp;Fayyaz Ahmad ,&nbsp;Nimra Nadeem ,&nbsp;Anum Javaid","doi":"10.1016/j.ultsonch.2025.107300","DOIUrl":"10.1016/j.ultsonch.2025.107300","url":null,"abstract":"<div><div>The present study revealed the higher extraction potential of sustainable choline chloride (ChCl) and ethylene glycol (EG) based deep eutectic solvent (DES) from <em>Mentha arvensis</em> via microwave irradiation. The categorical boosting (CatBoost) machine learning model was applied to optimize the extraction process against time (4–8 min), microwave power (160–320 W), and biomass quantity (1–2.0 g/10 mL) with DES. The experimentally optimized TPC 124 ± 4.0 <!--> <!-->mg GAE/g, TFC 79 ± 3.0 <!--> <!-->mg QE/g, and DPPH radical inhibition 90 ± 4.0 % evaluated in 6 min at 240 W with 1.0 g biomass. The lowest average relative errors of 0.402 % (TPC), 0.863 % (TFC), and 0.597 % (DPPH) for train and 0.679 % (TPC), 0.685 % (TFC) and 0.480 % (DPPH) for test data showed the consistency with the predicted values. The partial dependence and feature importance revealed the contributing impact of parameters for optimizing the extraction. The average contribution percentage of each predictor to the responses revealed that time contributed 32.5 % (TPC), 35.9 % (TFC), and 18.6 % (DPPH); microwave power contributed 26.7 % (TPC), 25.5 % (TFC), and 44.2 % (DPPH); while biomass contributed 40.8 % (TPC), 38.6 % (TFC), and 37.2 % (DPPH). The significant antibacterial (<em>S. aureus</em> = 25.5 ± 1.4 mm and <em>E. coli</em> = 23.5 ± 1.4 mm) with MICs (<em>S. aureus</em> = 50 ± 2.5 µg/mL and <em>E. coli</em> = 100 ± 1.5 µg/mL) and antifungal potential (<em>F. solani</em> = 22.5 ± 1.4 <!--> <!-->mm, <em>A. niger</em> = 23.5 ± 0.8 mm), with MIC (<em>F. solani</em> = 100 ± 0.4 µg/mL and <em>A. niger</em> = 50 ± 0.5 µg/mL) of optimized extracts recorded by DES. The DES would be the best alternative to traditional organic solvents based on higher extraction efficiency and sustainability.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"115 ","pages":"Article 107300"},"PeriodicalIF":8.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted preparation of shikonin-loaded emulsions for the treatment of bacterial infections","authors":"Xiaomiao Cui ,&nbsp;Zhiliang Gao ,&nbsp;Xinxin Han ,&nbsp;Qun Yu ,&nbsp;Vitoria H. Cauduro ,&nbsp;Erico M.M. Flores ,&nbsp;Muthupandian Ashokkumar ,&nbsp;Xiaoyong Qiu ,&nbsp;Jiwei Cui","doi":"10.1016/j.ultsonch.2025.107302","DOIUrl":"10.1016/j.ultsonch.2025.107302","url":null,"abstract":"<div><div>Bacteria can encapsulate themselves in a self-generated matrix of hydrated extracellular polymeric substances such as polysaccharides, proteins, and nucleic acids, thereby forming bacterial biofilm infections. These biofilms are drug resistant and will diminish the efficacy of antimicrobial agents, rendering treatment of such infections challenging. Herein, an innovative strategy is proposed to synergistically degrade bacterial biofilms and eradicate the entrapped bacteria through integrating α-amylase (α-Amy), shikonin (SK) and epigallocatechin gallate (EGCG) within an emulsion. The natural protein α-Amy is deployed to enzymatically hydrolyze the polysaccharide of biofilms. Due to the amphipilic properties of α-Amy and the cross-linking capability of EGCG, the formed α-Amy/SK@EGCG emulsion possess high stability. SK was encapsulated within the emulsion through ultrasound-assisted assembly, targeting to treat bacterial infection after biofilm degradation. <em>In vitro</em> and <em>in vivo</em> experiments demonstrate that the polyphenol-protein stabilized emulsion loaded with antibacterial SK achieves profound penetration into the biofilms due to the extracellular polysaccharide hydrolysis mediated by α-Amy. As a result, the α-Amy/SK@EGCG emulsion can significantly alleviate inflammation symptoms and accelerate the healing process of biofilm-infected wounds. This study provides a promising therapeutic strategy for the development of novel materials aimed for the enhanced treatment of bacterial biofilm infections.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"115 ","pages":"Article 107302"},"PeriodicalIF":8.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143561692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermosonication enhanced the bioactive, antioxidant, and flavor attributes of freshly squeezed tomato juice","authors":"Limei Li ,&nbsp;Hang Su ,&nbsp;Lingling Pang ,&nbsp;Yanfang Pan ,&nbsp;Xihong Li ,&nbsp;Qing Xu ,&nbsp;Jitian Song ,&nbsp;Liping Qiao","doi":"10.1016/j.ultsonch.2025.107299","DOIUrl":"10.1016/j.ultsonch.2025.107299","url":null,"abstract":"<div><div>The sterilization of freshly squeezed juices typically results in a compromise to the natural flavor, bio-active components, and nutritional value. Developing a new sterilization method for controlling the diverse effects has emerged as a significant challenge. This research aims to explore the potential application of thermosonication (TS) for freshly squeezed tomato juice. Results revealed that both TS (temperature 50, 60, and 70 ℃; time 5, 10, and 15 min) and thermal pasteurization (TP, 85 ℃/10 min) effectively inactivated microorganisms. No significant differences were observed in the basis properties (pH, total soluble solids (TSS) and titratable acidity (TA)) of tomato juice. Notably, TS effectively enhanced the juice quality, and the optimal condition is TS 60 ℃ 15 min. Its retention rates in color and suspension stability were greatly enhanced. Meanwhile, TS (60 ℃, 15 min) not only significantly increased lycopene content (42.13 %), ascorbic acid content (36.64 %), flavonoids content (33.94 %), and total phenols content (34.06 %), but also maintain a higher antioxidant capacity compared to PJ samples. Moreover, the sensory quality and volatile substances of TS treated were enhanced. It can be inferred that TS through ultrasonic cavitation ensured the microbial safety, improved nutritional value and sensory quality of tomato juice. This report provide a basis that a proper lower pasteurization temperature produced better effect in tomato juice when combined with ultrasound.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"115 ","pages":"Article 107299"},"PeriodicalIF":8.7,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-cavitation-enhanced drug delivery via microbubble clustering induced by acoustic vortex tweezers","authors":"Ching-Hsiang Fan ,&nbsp;Elaine Huang ,&nbsp;Wei-Chen Lo ,&nbsp;Chih-Kuang Yeh","doi":"10.1016/j.ultsonch.2025.107273","DOIUrl":"10.1016/j.ultsonch.2025.107273","url":null,"abstract":"<div><div>The application of acoustic vortex tweezers (AVT) in conjunction with ultrasound (US) cavitation pulses presents a promising noninvasive approach for the delivery of high concentrations of therapeutic agents. This methodology facilitates the aggregation of drug-loaded microbubbles (MBs) into clusters, which are subsequently destroyed to release their contents. Nevertheless, prior investigations have not thoroughly examined the resonance frequency and cavitation activity of MB clusters, critical factors that could enhance the efficiency of payload release. Theoretically, the resonance frequency of an MB cluster is expected to approximate that of a single large bubble of comparable size, thus being significantly lower than that of the individual MBs constituting the cluster. Accordingly, this study aims to optimize the release of payloads from AVT-trapped MB clusters, which measure 15 to 40 μm (mean radius: 24.7 μm) in size, by employing US at their resonance frequency of 100 kHz, henceforth referred to as “on-resonance US.” In this investigation, MBs were loaded with the model drug DiI, resulting in the formation of DiI-MBs, which were then clustered utilizing AVT. On-resonance US excitation was subsequently applied to enhance the release of the drug payload. The dimensional characteristics of the DiI-MB clusters formed via 3-MHz AVT were measured to determine the range of resonance frequencies. Concurrent optical and acoustic analyses were conducted to evaluate the size, oscillation dynamics, and cavitation activity of the DiI-MB clusters in response to on-resonance US excitation. Additionally, the payload release from these clusters was quantitatively assessed. Our results indicate that significant oscillations of individual DiI-MB clusters commenced at a pressure of 44 kPa during 100 kHz US excitation. Further quantitative experiments demonstrated that the synergistic combination of AVT and 100-kHz US at 65 kPa significantly enhanced the payload release efficiency to 93 %. This efficiency surpassed that achieved with either method independently, with increases of 1.8-fold relative to AVT alone and 2.3-fold compared to 100-kHz US alone. The acoustic analyses revealed the onset of inertial cavitation at 44 kPa, which strongly correlated with payload release efficiency (R² = 0.78). These findings underscore the potential of our proposed methodology in monitoring and enhancing the efficiency of drug release.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"114 ","pages":"Article 107273"},"PeriodicalIF":8.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bubble cloud-mediated cavitation for tumor mechanical ablation and effector immune cell deployment","authors":"Jing Cao ,&nbsp;Ling Wang ,&nbsp;Jiarui Li ,&nbsp;Mengyu Song ,&nbsp;Yinuo Zheng ,&nbsp;Xiangling He ,&nbsp;Xiaoying Li ,&nbsp;Songcheng Xu ,&nbsp;Litao Sun","doi":"10.1016/j.ultsonch.2025.107296","DOIUrl":"10.1016/j.ultsonch.2025.107296","url":null,"abstract":"<div><div>Histotripsy is a cavitation-based tumor ablation technology. To achieve precise cavitation-based ablation requires investigating the cavitation behavior of the bubble cloud and their impact on tumor tissue. This study explored the cavitation behavior of bubble clouds generated by perfluoropentane (PFP)-loaded nanodroplets and efficacy of bubble cloud cavitation in tumor ablation under varying ultrasound intensities. PFP-loaded nanodroplets (∼200 nm) were employed as exogenous cavitation nuclei to reducing the required ultrasound energy for activation of bubble cloud. We investigated the formation, vibration, and collapse of bubble clouds in solution and phantom models under varying ultrasound intensities. Results indicated distinct cavitation patterns: (1) Nanodroplets slowly vaporized and formed continuously vibrating bubble clouds; (2) Nanodroplets rapidly vaporized and resulted in quickly collapsing bubble clouds. At both the cellular and animal levels, cavitation ablation efficacy was examined, revealing that all bubble cloud cavitation patterns could induce immunogenic cell death (ICD), promoting the release of damage-associated molecular patterns (DAMPs) and triggering effector immune cell deployment of peripheral immune response and local tumor infiltration. During the treatment, the ultrasound intensity of 0.5 W/cm² had the highest level of central tumor CD8⁺ T cell infiltration. The conclusion was that sustained bubble cloud oscillation, rather than rapid vaporization and rupture, proved more beneficial for antitumor therapy, particularly in enhancing the local infiltration of effector immune cells.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"115 ","pages":"Article 107296"},"PeriodicalIF":8.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143529289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Ultrasound-assisted low-sodium salt curing to modify the quality characteristics of beef for aging” [Ultrason. Sonochem. 111 (2024) 107134]","authors":"Lei Liu ,&nbsp;Fengnan Niu ,&nbsp;Yating Xiong ,&nbsp;Peng Wang ,&nbsp;Xiaoyu Lyu ,&nbsp;Zongyun Yang","doi":"10.1016/j.ultsonch.2025.107225","DOIUrl":"10.1016/j.ultsonch.2025.107225","url":null,"abstract":"","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"114 ","pages":"Article 107225"},"PeriodicalIF":8.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the mechanisms of ultrasonic radiation-induced free radical stress on algal communities: Insights into growth inhibition, photosynthetic disruption, and antioxidant defense responses","authors":"Xiaoge Wu ,&nbsp;Tingting Shen ,&nbsp;Xiaoyang Liu ,&nbsp;Guangming Zhang ,&nbsp;Xiaoqing Qian ,&nbsp;Wenlan Yang","doi":"10.1016/j.ultsonch.2025.107297","DOIUrl":"10.1016/j.ultsonch.2025.107297","url":null,"abstract":"<div><div>Algal blooms pose a significant threat to global environmental health, compromising water quality and public safety. Ultrasonic radiation has emerged as a promising, eco-friendly strategy for controlling these blooms, but the underlying mechanisms remain unclearly understood. This study investigated the effects of ultrasonic radiation on the growth, photosynthetic performance, and antioxidant defense systems of an algal mixture over a 5-day period. Analysis techniques, including scanning electron microscopy (SEM), excitation-emission matrix (EEM) analysis, and transcriptomic profiling, were employed to elucidate the multifaceted responses of algal cells to ultrasonic treatment. Ultrasonic radiation induced significant free radical generation, primarily hydroxyl radicals (·OH), which played a critical role in cellular damage. Within 24 h, treatment led to a 50% reduction in algal cell counts, a 30% decline in chlorophyll-a levels, and a 25% decrease in photosynthetic efficiency. Phycocyanin, a vital pigment for cyanobacteria, exhibited heightened sensitivity to a single ultrasonic treatment, while subsequent treatments showed no additional reduction, suggesting that <em>Microcystis aeruginosa</em> is particularly susceptible to the ultrasonic damage. EEM analysis revealed significant changes in the fluorescence intensity of extracellular organic matter (EOM) and intracellular organic matter (IOM) peaks, indicative of oxidative stress and metabolic disruption. Transcriptomic analysis of <em>Microcystis aeruginosa</em> revealed a profound reprogramming of gene expression in response to sonication. Stress response genes, particularly those involved in antioxidant defense, were upregulated, while photosynthesis-related genes were downregulated. Our research indicates that short-term ultrasonic radiation has a long-term stress effect on algal cells, and this might be able to prevent the tendency of cyanobacteria blooms.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"115 ","pages":"Article 107297"},"PeriodicalIF":8.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass transfer of acoustic cavitation bubbles in multi-bubble environment","authors":"Kanji D. Hattori,&nbsp;Takuya Yamamoto","doi":"10.1016/j.ultsonch.2025.107295","DOIUrl":"10.1016/j.ultsonch.2025.107295","url":null,"abstract":"<div><div>The mass transfer around acoustic cavitation in a multi-bubble environment was numerically studied. To model the multi-bubble environment, several bubbles were placed at the vertices of the polyhedron, and one bubble was placed at the center, and then an ultrasonic pressure wave with a frequency of 20 kHz was imposed along the simulation boundary box. In this study, the mass transfer of chemical species that were initially present only in the bubbles was investigated. The numerical simulation revealed that the mass transfer to the outside bubble was enhanced by the bubble translational motion caused by the secondary Bjerknes force, whereas the mass transfer to the outside bubble increased during the bubble compression period, and the mass transfer to the inside bubble increased during the bubble expansion period. In addition, the mass transfer to the outside bubble was enhanced, particularly for the nonspherical bubble motion during the bubble compression period. The mass transfer to the outside bubble increases with a denser bubble arrangement, which can be explained in terms of the cover ratio, which is defined as the ratio of the projected area of the surrounding bubbles to the central bubble.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"115 ","pages":"Article 107295"},"PeriodicalIF":8.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143551173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound and lactic/malic acid treatment for mitten crab decontamination: Efficacy and mechanisms against A. hydrophila","authors":"Han Huang ,&nbsp;Yanjun Tong ,&nbsp;Xiaomei Lyu ,&nbsp;Wei Zhao ,&nbsp;Ruijin Yang","doi":"10.1016/j.ultsonch.2025.107294","DOIUrl":"10.1016/j.ultsonch.2025.107294","url":null,"abstract":"<div><div>The Chinese mitten crab (<em>Eriocheir sinensis</em>), recognized as a high-value aquatic product, necessitates effective cleaning to ensure both safety and quality. Conventional cleaning methods frequently fail to eliminate biofilms and pathogenic bacteria, such as <em>Aeromonas hydrophila</em>, which pose significant health risks and contribute to spoilage. This study explores the bactericidal efficacy and underlying mechanisms of ultrasound treatment combined with a lactic acid and malic acid complex solution for decontaminating crabs and enhancing food safety. Employing a range of methodologies, including microscopic imaging, live/dead staining, RT-qPCR, and texture and microstructure analysis, the results indicate that the combined treatment significantly reduced <em>A. hydrophila</em> counts by 4.16 lg CFU/mL and induced substantial bacterial membrane damage, as evidenced by scanning electron microscopy (SEM). Gene expression analysis revealed a pronounced downregulation of biofilm-related genes. Notably, the treatment also preserved the texture and sensory properties of crab meat, thereby ensuring high product quality. These findings suggest that the application of ultrasound in conjunction with a lactic acid-malic acid solution represents a green and effective strategy for improving food safety and quality in the processing of aquatic products, offering a sustainable and eco-friendly alternative to traditional cleaning methods.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"115 ","pages":"Article 107294"},"PeriodicalIF":8.7,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143526559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}