Ultrasonics Sonochemistry最新文献

{"title":"Comparative analysis of high-intensity non-thermal physical treatments on the structural and functional properties of Spirulina platensis protein","authors":"Junwen Wang ,&nbsp;Qinhao Guan ,&nbsp;Zhiting Liu ,&nbsp;Jiangfei Li ,&nbsp;LiBaiyi Zhou ,&nbsp;Qiaoyun Qi ,&nbsp;Yue Zhang ,&nbsp;Elena Ibañez ,&nbsp;Alejandro Cifuentes ,&nbsp;Weihong Lu","doi":"10.1016/j.ultsonch.2025.107623","DOIUrl":"10.1016/j.ultsonch.2025.107623","url":null,"abstract":"<div><div><em>Spirulina platensis</em> protein (SPP) has attracted attention as a sustainable alternative to conventional proteins. However, its structural and functional performance under different processing conditions remains underexplored. This study investigated the effects of three non-thermal physical treatments—high hydrostatic pressure (HHP, 100–600 MPa), high-intensity ultrasound (HIU, 70–100 % amplitude), and high-speed shear homogenization (HSS, 1.0–2.5 W)—on the structure and functionality of SPP. SDS-PAGE revealed treatment-specific alterations in protein subunits, accompanied by distinct changes in sulfhydryl/disulfide, surface hydrophobicity, intrinsic fluorescence, and secondary structure. HIU induced the strongest structural disruption, characterized by reduced α-helix content, exposure of hydrophobic residues, and a pronounced decrease in particle size and ζ-potential, whereas HHP promoted unfolding at moderate pressures but aggregation at higher levels. HSS primarily reduced particle size with moderate effects on unfolding. Collectively, these results indicate that high-Intensity non-Thermal physical treatments effectively modulate the functional properties of SPP, demonstrating its potential as a protein ingredient for food applications.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"122 ","pages":"Article 107623"},"PeriodicalIF":9.7,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145312281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of emulsion stabilized by an ultrasonically prepared protein-polyphenol-polysaccharide complex: structure, functional properties and interfacial behavior.","authors":"Yuyang Huang, Baoning Zheng, Bingyu Sun, Ying Zhu, Linlin Liu, Jiyuan Liu, Yixin Zhang, Yang Li, Xiuqing Zhu","doi":"10.1016/j.ultsonch.2025.107622","DOIUrl":"https://doi.org/10.1016/j.ultsonch.2025.107622","url":null,"abstract":"<p><p>Based on the mechanism that polyphenols and polysaccharides can modulate protein conformation through non-covalent interactions such as hydrogen bonding and hydrophobic forces, the construction of ternary complexes offers a promising strategy for developing efficient emulsion-based delivery systems. This study employed soy protein isolate (SPI), (-)-epigallocatechin gallate (EGCG), and polydextrose (PD) as raw materials to fabricate SPI, SPI-EGCG, SPI-PD, and SPI-EGCG-PD complexes via ultrasonic treatment. These complexes were then used to stabilize emulsions, and their structural characteristics, functional properties, and interfacial behavior were systematically investigated. The results indicated that the protein secondary structure of the ultrasonicated samples underwent significant alterations compared to SPI and the complexes (SPI-EGCG, SPI-PD, SPI-EGCG-PD). Specifically, the contents of α-helix and β-sheet decreased significantly, while the content of random coils increased significantly. Also, their emulsifying capacity and antioxidant activity were both significantly enhanced (p < 0.05). The emulsion stabilized by the ultrasonically prepared SPI-EGCG-PD complex exhibited superior stability, as evidenced by the highest emulsion stability index (89.6 min), the smallest particle size (599.6 nm), the highest absolute ζ-potential value (-37.4 mV), the greatest interfacial adsorbed protein content (4.89 mg/mL), the best oxidative stability (5.31 μmol/L), the lowest interfacial tension (20.89 mN/m), and the best storage stability. This study elucidates the mechanism by which ultrasonic treatment promotes the synergistic adsorption of proteins, polyphenols, and polysaccharides. It further examines the role of the interfacial behavior of ultrasonicated proteins and their complexes in maintaining emulsion stability, thereby providing a theoretical foundation for developing highly stable delivery systems for bioactive substances.</p>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"122 ","pages":"107622"},"PeriodicalIF":9.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145353288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From lab to pilot: sonochemical coating of PDDA-based polymer dots for scalable, wash-durable antibacterial textiles","authors":"Garima Rathee,&nbsp;Antonio Puertas-Segura,&nbsp;Jeniffer Blair,&nbsp;Tzanko Tzanov","doi":"10.1016/j.ultsonch.2025.107619","DOIUrl":"10.1016/j.ultsonch.2025.107619","url":null,"abstract":"<div><div>Poly(diallyldimethylammonium chloride) (PDDA) is a cationic polymer with strong antibacterial activity, but its coating applications are hindered by limited stability and dose-dependent cytotoxicity. Nanoformulating PDDA into polymer dots (PDDA-based PDs) via hydrothermal synthesis with boric acid offers enhanced biocompatibility, stability, and antimicrobial efficacy. The synthesised PDs demonstrated potent antibacterial activity against Gram-positive (<em>S. aureus</em>) and Gram-negative bacteria (<em>E. coli</em> and <em>P. aeruginosa</em>) with MIC values of 0.056–0.112 mg/mL and superior biofilm inhibition capabilities. Significantly lower antimicrobial resistance development was observed, with only 4–8 fold MIC increase after 30 days compared to 128–2048 fold increase for conventional antibiotics. Sonochemical coating onto cotton fabrics achieved exceptional washing durability, retaining strong antibacterial activity even after 60 high-temperature hospital laundry cycles with log reductions of 4.72 for <em>S. aureus</em>, 2.31 for <em>P. aeruginosa</em>, and 3.26 for <em>E. coli</em>. The multi-targeted antimicrobial mechanism of the PDs involves ROS generation, disruption of bacterial metabolism, and physical damage to membrane integrity. The coating process was successfully upscaled using a roll-to-roll sonochemical pilot system, demonstrating industrial feasibility. PDDA-based PDs offer a promising platform for developing sustainable, wash-durable antimicrobial textiles suitable for healthcare applications.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"122 ","pages":"Article 107619"},"PeriodicalIF":9.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound cavitation modulates intracellular ROS and gene expression in antibacterial sonodynamic therapy","authors":"Xinyu Li ,&nbsp;Fengmeng Teng ,&nbsp;Fengmin Wu ,&nbsp;Changlong Li ,&nbsp;Hui Zhang ,&nbsp;Chunbin Zhang ,&nbsp;Dong Zhang","doi":"10.1016/j.ultsonch.2025.107620","DOIUrl":"10.1016/j.ultsonch.2025.107620","url":null,"abstract":"<div><div>Antibacterial sonodynamic therapy (aSDT) has emerged as a promising non-invasive antimicrobial modality utilizing ultrasound activation. This study employed curcumin (CUR) as a sonosensitizer to systematically investigate the mechanisms underlying aSDT. We first examined ultrasound (US)-induced intracellular reactive oxygen species (ROS) generation and subsequently evaluated the bactericidal enhancement mediated by microbubble-augmented cavitation, including membrane disruption and apoptotic pathways. A comprehensive experimental approach was implemented, including: (1) bacterial viability assays on solid media coupled with biomass quantification, (2) flow cytometric apoptosis detection, (3) intracellular ROS measurement, (4) gene expression changes through RNA-seq and qRT-PCR, and (5) scanning electron microscopy (SEM) analysis of Pseudomonas aeruginosa morphology. Results demonstrated a dose-dependent relationship between cavitation intensity and antibacterial efficacy. Although US can stimulate intracellular ROS elevation, experimental results indicated that aSDT operates primarily through two mechanisms: ultrasound-induced mechanical disruption of cell membranes, and apoptosis triggered by extracellular ROS generated via US/sonosensitizer synergy that enter cells. Genomic analysis further elucidated that aSDT-induced bacterial apoptosis originated from the synergistic effect of ultrasonic cavitation and sonosensitizer. It differentially regulated the expression of key genes in P. aeruginosa (downregulating PA4211, PA0876, PA3361 and upregulating PA3570, PA2433, PA4880), damaged the biofilm structure and inhibited its remodeling. Meanwhile, it disrupted the redox homeostasis and metabolic balance, utimately leading to irreversible damage to the cell membrane and a massive accumulation of intracellular ROS.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"122 ","pages":"Article 107620"},"PeriodicalIF":9.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145321686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water-based ultrasonic pretreatment enhances moso bamboo dimensional stability and mildew resistance","authors":"Jing Qian ,&nbsp;Pengfei Xia ,&nbsp;Shixia Cui ,&nbsp;Zekai Sun ,&nbsp;Taorong Cheng ,&nbsp;Bao Sheng ,&nbsp;Katherine Semple ,&nbsp;Majid Mokarizadehhaghighishirazi ,&nbsp;Chunping Dai ,&nbsp;Jiejie Sun","doi":"10.1016/j.ultsonch.2025.107621","DOIUrl":"10.1016/j.ultsonch.2025.107621","url":null,"abstract":"<div><div>Bamboo is naturally susceptible to mould and dimensional instability under humid conditions, which limits its durability in practical applications. This study aimed to determine whether water-based ultrasonic pretreatment could improve moso bamboo’s (<em>Phyllostachys edulis</em>) dimensional stability and mildew resistance by altering microstructure and physicochemical properties. Results showed that ultrasonic pretreatment increased the mass loss rate by 0.2–0.8 %. It reduced the hot-water extractive content by 6–7 %, and decreased absolute-dry density by 0.02–0.06 g/cm³. The treatment caused pit membrane rupture and parenchyma wall thinning (the distribution range narrowed to approximately 5.5–12.5 μm at 20–60 min), and removing amorphous components enhanced cellulose crystallinity by about 8.4 % and slightly reduced microfibril angle by 1.8 % (both at 10 min). These microstructural and physicochemical changes led to improved dimensional stability with about 0.2 % reduction in radial swelling, despite slightly higher moisture uptake. More importantly, mould resistance improved significantly. Mould infection decreased by about 20 % for <em>A. niger</em> (to around 45 % at 30 min) and about 83 % for <em>P. citrinum</em> (to about 10 % at 60 min). The findings demonstrate good potential for ultrasonic pretreatment as a green, non-chemical method to enhance moso bamboo’s dimensional stability and mildew resistance. Ultrasonic pretreatment could also be combined with other modification strategies to achieve superior performance in demanding service environments.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"122 ","pages":"Article 107621"},"PeriodicalIF":9.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel ultrasonic field-focusing nozzle for enhanced sonochemical reactor performance","authors":"Abdulmajeed Baker,&nbsp;Oualid Hamdaoui,&nbsp;Lahssen El Blidi,&nbsp;Abdulaziz Alghyamah","doi":"10.1016/j.ultsonch.2025.107617","DOIUrl":"10.1016/j.ultsonch.2025.107617","url":null,"abstract":"<div><div>Ultrasonic reactors operating at megahertz frequencies typically exhibit low cavitation intensity and sonochemical yields. This study introduces an innovative ultrasonic field-focusing nozzle that improves the performance of a 1.7 MHz sonoreactor by concentrating the acoustic field geometrically into a defined focal zone. The sonochemical performance of the reactor with and without the focusing nozzle was compared across solution volumes ranging from 50 to 200 mL. Comprehensive characterization techniques were employed, including calorimetry, KI and Fricke dosimetries, hydrogen peroxide quantification, 4-nitrophenol oxidation, Sunset Yellow FCF (SSY) degradation kinetics, and sonochemiluminescence (SCL) imaging. The focused configuration outperformed the unfocused system across all metrics. Calorimetric power increased by up to 15 %, and radical production rates, as measured by triiodide, Fe³⁺, and 4-nitrocatechol yields, rose by over 50 % under optimal conditions. SSY degradation rates improved by as much as 78.5 %, and SCL imaging revealed a bright, narrowly confined cavitation zone, which is indicative of elevated local pressure amplitudes. The most significant enhancements occurred at a liquid volume of 80 mL (liquid height of 11.3 cm). This corresponded to the upper boundary of the transducer’s Fresnel (near-field) zone. In this zone, standing wave coherence and constructive interference are maximized. Even at larger volumes (up to 200 mL), the focusing nozzle sustained significant improvements in cavitational activity and sonochemical yield. These results highlight the importance of geometric focusing in overcoming the limitations of high-frequency ultrasonication, especially when acoustic energy would otherwise diminish.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"122 ","pages":"Article 107617"},"PeriodicalIF":9.7,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145312279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of natural deep eutectic solvents’ hydration level, choice of hydrogen bond donor and application of ultrasound on the extraction, anti-nutritional components, structural properties and functionality of canola protein isolates","authors":"Abouzar Karimi ,&nbsp;Juwuan Choi ,&nbsp;Anusha Samaranayaka ,&nbsp;Pankaj Bhowmik ,&nbsp;Lingyun Chen","doi":"10.1016/j.ultsonch.2025.107618","DOIUrl":"10.1016/j.ultsonch.2025.107618","url":null,"abstract":"<div><div>In a systematic attempt to improve the cost-effectiveness and knowledge of the topic of plant protein extraction using natural deep eutectic solvents (NDESs), 18 extraction treatments, half of which paired with sonication, were performed to extract canola protein isolates using two groups of NDESs: choline chloride-glucose-water (CG) and choline chloride-water (C), with water contents ranging from 30 to 90 %. The highest protein extraction efficiencies were observed at water contents of 60 % for CG group (50.28 %) and 90 % for C group (45.48 %), while sonication improved these efficiencies to 60.68 % and 58.11 %, respectively. Increased solvent hydration and sonication also effectively reduced phenolic compounds and phytic acid contents, especially in the CG group. Despite increasing the solvents’ viscosity and density, the combination of glucose and water as hydrogen bond donors proved more effective for protein extraction than water alone, especially for larger fractions such as cruciferins and aggregates. Furthermore, while protein secondary structure remained mostly intact, variations in solvents’ water content, and sonication, affected the tertiary structure and particle size distribution, with the strength and flexibility of the NDESs’ nanostructure possibly affecting the protein conformation and aggregation. Regarding functionality, sonicated isolates showed an average of 8.31 % lower aqueous solubility across the pH range of 3–7, along with more than double the emulsion stability and a lower foam stability at pH 3, compared to non-sonicated isolates. Overall, pushing NDESs to their upper hydration limit, proper selection of hydrogen bond donors, and the application of ultrasound can improve the cost-effectiveness and quality of extracted canola protein isolates.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"122 ","pages":"Article 107618"},"PeriodicalIF":9.7,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roughness-engineered potential wells for controllable ultrasonic cavitation: Multistage bubble capture–release mechanism revealed by multiscale experiments and simulations","authors":"Yibo Suo ,&nbsp;Xijing Zhu ,&nbsp;Chenglong Bi ,&nbsp;Linzheng Ye ,&nbsp;Jing Li ,&nbsp;Zuoxiu Li ,&nbsp;Xiangmeng Li ,&nbsp;Quan Cheng","doi":"10.1016/j.ultsonch.2025.107610","DOIUrl":"10.1016/j.ultsonch.2025.107610","url":null,"abstract":"<div><div>Controlling cavitation dynamics is essential for optimizing ultrasonic-assisted processing, targeted energy release, and damage mitigation. Here, we propose a structural strategy for tunable cavitation control using roughness-engineered surfaces that generate geometry-induced potential wells. Titanium alloy walls with varying porosity were fabricated via triply periodic minimal surface designs to systematically modulate roughness. High-speed imaging revealed that moderate roughness stabilizes bubble aggregation above the surface, whereas smooth walls fail to retain bubbles and excessive roughness induces perturbation-driven release. Specifically, in the 20 % porosity sample at t = 0.78 ms, multiple larger clusters formed at the center region of the wall, displaying asymmetric shapes and stretched edges, significantly increasing bubble retention time. Molecular dynamics simulations demonstrated that van der Waals–dominated short-range adsorption and localized low-energy zones extend bubble residence time, enabling stable capture. Excessive roughness, however, disrupts potential well uniformity, triggering asymmetric collapse and directed energy release. Integrating experimental and simulation results, we establish a multistage “capture–perturbation–collapse–release” framework for surface-induced cavitation control. This approach could potentially enable targeted cavitation control in ultrasonic cleaning, precision machining, and erosion prevention.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"122 ","pages":"Article 107610"},"PeriodicalIF":9.7,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An all-acoustic toolbox for performing unit operations and their combinations on levitated droplets","authors":"Jianqing Li,&nbsp;Nicholas J. Goddard,&nbsp;Khalid Haliru,&nbsp;Ruchi Gupta","doi":"10.1016/j.ultsonch.2025.107611","DOIUrl":"10.1016/j.ultsonch.2025.107611","url":null,"abstract":"<div><div>Automatic and contactless manipulation of droplets in mid-air has wide potential applications from materials processing to biochemistry and pharmaceutical development. To perform (bio)chemical reactions in a contactless and automated fashion, herein, we report an all-acoustic toolbox for performing unit operations (generate, hold, move and merge) on droplets with integrated optical detection. The toolbox was realized using phased arrays of ultrasound transducers in combination with pump and capillary nozzles to generate pendant droplets of required volumes and pulling them away from the tip of the nozzles using acoustic forces, then using the acoustic forces to move and merge droplets followed by quantitative measurements using the integrated optical detection. This is an unprecedented report on droplet generation by pulling pendant droplets using acoustic forces. The generation of aqueous droplets containing different percentages of dimethyl sulfoxide was possible up to 50 % DMSO. The error in the volume of aqueous droplets generated using the approach was between 5.6 and 13 % depending on the volume of the droplet and the transducer voltage. The success rate for holding droplets varied between 78 and 95 % depending on the droplet size and transducer peak-to-peak voltage. Up to six droplets in two columns of three could be generated, moved and merged. Subsequently, a sequence of unit operations was created to implement an esterase enzyme assay, which is widely used in biochemical labs and industrial processes, in levitated droplets. The reaction rate was higher in levitated droplets (2.17 to 5.21 × 10⁻³ s⁻¹ compared to that in microtiter plates (∼2.0 × 10⁻³ s⁻¹), which we hypothesize is a result of stirring of the droplet by acoustic streaming. Future work will focus on increasing the number of droplets that can be levitated and manipulated using our all-acoustic toolbox. The adoption of our all-acoustic toolbox for performing (bio)chemical reactions will not only increase automation but also reduce the use of single-use non-recyclable plastics such as microtiter plates and pipette tips.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"122 ","pages":"Article 107611"},"PeriodicalIF":9.7,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study on physiological and metabolic responses of crayfish to ozone micro-nano bubbles and ultrasound washing","authors":"Xinyan Tong ,&nbsp;Lingxiang Bao ,&nbsp;Hanbin Lin ,&nbsp;Yan Chen ,&nbsp;Xuanju Shen ,&nbsp;Yu Xia ,&nbsp;Nasra Seif Juma ,&nbsp;Qinyi Zeng ,&nbsp;Yadong Zhao","doi":"10.1016/j.ultsonch.2025.107616","DOIUrl":"10.1016/j.ultsonch.2025.107616","url":null,"abstract":"<div><div>Maintaining the well-being of living aquatic products before processing is crucial to guarantee best food quality. This study investigated the different effects of ozone micro-nano bubbles washing (OMW) and ultrasound washing on the well-being of living crayfish, one of the most economically valuable freshwater aquatic products in China. It has been found that OMW was a gentler and more effective alternative to traditional ultrasonic washing (UW) that reduced physical damage, thus leading to enhanced living vitality, increased intestinal content evacuation rate, and improved surface cleanliness of crayfish before processed as aquatic food. These improvements were attributed to the milder physical force generated by bubbles contraction and collapse than the cavitation and mechanical effects of UW. In addition, the strong cavitation, mechanical, and thermal effects of UW impaired the antioxidant system of crayfish, leading to the severe hepatopancreas damage as indicated by the substantially elevated activities of alanine aminotransferase and aspartate aminotransferase. Moreover, OMW significantly lowered the lactate dehydrogenase activity and reduced lactic acid accumulation due to decreased oxidative stress, thereby preventing acidosis in crayfish. These findings demonstrated that OMW was a milder yet more effective approach to improve the well-being of living aquatic products, thus maintaining their vitality and quality prior to processing into foods, suggesting its great potential as a pretreatment technology for applications in the aquatic food industry.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"122 ","pages":"Article 107616"},"PeriodicalIF":9.7,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}