Ultrasonics Sonochemistry最新文献

{"title":"A novel strategy for ultrasound-assisted immobilized enzyme hydrolysis for the preparation of yak bone collagen peptides: Process optimization, structure and antioxidant mechanisms","authors":"Yinjuan Cao ,&nbsp;Zhaoyang Song ,&nbsp;Yabin Ma ,&nbsp;Yulong Luo ,&nbsp;Jianrui Qi","doi":"10.1016/j.ultsonch.2025.107689","DOIUrl":"10.1016/j.ultsonch.2025.107689","url":null,"abstract":"<div><div>In recent years, food-derived antioxidant peptides have garnered growing interest within the food industry, owing to their remarkable bioavailability and safety profiles. In this study, collagen peptides derived from yak bone (YCP) were prepared using an ultrasound-assisted immobilized enzyme hydrolysis system (US + IP-EH), and a comprehensive investigation was conducted into their structural characteristics, stability, and underlying antioxidant mechanisms. The results revealed optimal extraction conditions as follows: an ultrasonic power of 415 W, an enzymatic hydrolysis temperature of 49 °C, a hydrolysis duration of 8.4 h, an immobilized enzyme dosage of 3.6 %, and hydrolysis degree (DH) reaching 22.36 % in the validation experiment. Notably, the US + IP-EH approach significantly enhanced the yield, structural integrity, and antioxidant capacity of YCP, enabling them to retain robust antioxidant stability even under challenging conditions such as high temperatures, extreme pH (strong acids or alkalis), light exposure, and gastrointestinal digestion. Furthermore, ten novel antioxidant peptides were successfully identified, demonstrating non-allergenic and non-toxic properties. Among these, peptides P1, P4, and P10 exhibited favorable interactions with Keap1 by occupying its primary binding sites, thereby effectively disrupting the Keap1-Nrf2 complex structure. This research provides a solid theoretical and practical foundation for the development of yak bone collagen into natural functional foods and health products.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107689"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145560294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonically synthesized Cu/Ag/Zn nanocomposite coatings for antibacterial and antibiofilm applications in urological devices","authors":"Houhong Wang ,&nbsp;Chun Luo ,&nbsp;Wenli Chen ,&nbsp;Weilin Wang ,&nbsp;Yongyun Chen ,&nbsp;Kelei Shang ,&nbsp;Yan Peng ,&nbsp;Zhongmin Li","doi":"10.1016/j.ultsonch.2025.107674","DOIUrl":"10.1016/j.ultsonch.2025.107674","url":null,"abstract":"<div><div>Catheter-associated urinary tract infections (CAUTIs) pose a persistent clinical challenge due to bacterial colonization and biofilm formation on indwelling urological devices. This study investigates the efficacy of Cu/Ag/Zn trimetallic nanocomposites (TMNCs) as antimicrobial coatings for urinary catheters, synthesized via a green, ultrasonic–autoclave-assisted method. The nanocomposites were thoroughly characterized using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), Brunauer–Emmett–Teller (BET) surface area analysis, and field emission scanning electron microscopy (FESEM) to confirm their mesoporous, crystalline, uniformly distributed, and thermally stable nanoparticles. An optimized ultrasonic embedding technique was employed to uniformly coat TMNCs onto commercial urinary catheters, ensuring strong adhesion and consistent coverage. Antibacterial assays demonstrated concentration-dependent inhibition zones, reaching up to 21.5 mm for <em>Escherichia coli</em> and 24.0 mm for <em>Staphylococcus aureus</em>. Minimum inhibitory concentration (MIC) tests confirmed potent bactericidal activity, with MIC values of 32 µg/mL for <em>E. coli</em> and 64 µg/mL for <em>S. aureus</em>. Anti-biofilm assessments revealed that TMNCs matched or outperformed the efficacy of vancomycin, achieving up to 86.12 % inhibition for <em>S. aureus</em> and 73.59 % for <em>E. coli</em> at 2 × MIC. Cytotoxicity testing using U87 glioblastoma cells indicated good biocompatibility, with over 79 % cell viability at 0.1 mg/mL, followed by a dose-dependent decline at higher concentrations. Long-term stability studies conducted over 60 days under varied storage conditions confirmed the photothermal and colloidal stability of the TMNCs. In conclusion, Cu/Ag/Zn TMNC-coated urinary catheters demonstrated excellent antibacterial and anti-biofilm properties, biocompatibility, and long-term stability. These multi-functional coatings present a promising strategy for reducing CAUTI incidence and improving patient safety and clinical outcomes in urological applications.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107674"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145499216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the pathways of flavor formation in marinated lamb driven by ultrasound-assisted vacuum tumbling: An integrated multi-omics approach","authors":"Chenchen Xu ,&nbsp;Shouwei Wang ,&nbsp;Yan Zhao ,&nbsp;Jiapeng Li ,&nbsp;Shunliang Zhang ,&nbsp;Jing Bai ,&nbsp;Yuxuan Shi ,&nbsp;Xianqi Yao ,&nbsp;Anyu Gao ,&nbsp;Bing Zhao","doi":"10.1016/j.ultsonch.2025.107677","DOIUrl":"10.1016/j.ultsonch.2025.107677","url":null,"abstract":"<div><div>The development of desirable flavor in processed lamb remains a significant challenge for the meat industry. This study aimed to investigate the effects of ultrasound-assisted vacuum tumbling (UVT) on the flavor characteristics of lamb using a combined analytical approach of flavoromics, lipidomics, and metabolomics. The results indicated that UVT significantly expedited the degradation and subsequent oxidation of crucial flavor precursors, including phospholipid metabolites (phosphatidylethanolamine, phosphatidylserine, cytidine diphosphate-diglyceride, phosphatidylcholine, and lysophosphatidylcholine), glyceride metabolites (triacylglycerols), nucleotide metabolites (inosine monophosphate and guanosine monophosphate), and amino acid metabolites (L-glutamine). This acceleration was associated with the formation of desirable flavor compounds in the marinated lamb, including Hexanal, 1-Octen-3-ol, Methional, 2-Acetylfuran, and various esters. In conclusion, this study demonstrates that UVT is a powerful strategy for intensifying and controlling the flavor profile of meat products, providing a robust theoretical foundation for the precise application of ultrasound technology in the modern meat industry.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107677"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145515824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of thermo-cylindrical type focused-ultrasound as novel milk pasteurization: microbial inactivation, immunoglobulin G retention, and physicochemical characteristics","authors":"Prabhathma Yasasvi Rathnayake ,&nbsp;Soyeong Kim ,&nbsp;Rina Yu ,&nbsp;Chemin Nam ,&nbsp;Sin-Young Park ,&nbsp;Seonae Hwangbo ,&nbsp;Hae In Yong","doi":"10.1016/j.ultsonch.2025.107615","DOIUrl":"10.1016/j.ultsonch.2025.107615","url":null,"abstract":"<div><div>This study aimed to investigate the efficacy of focused ultrasound as a novel milk pasteurisation technology to replace conventional low-temperature long-time (LTLT) method. The microbial inactivation, immunoglobulin G (IgG) retention, and physicochemical properties of milk treated with focused ultrasound were evaluated. Thermo-cylindrical type (55 °C) focused ultrasound was used for all experiments. Milk was subjected to focused ultrasound at 30 W and 100 W for 0, 10, 20, and 30 min to investigate the antimicrobial effects of this method. The total aerobic bacteria, coliforms, <em>Escherichia coli</em> O157:H7, and <em>Listeria monocytogenes</em> counts decreased (p &lt; 0.05) with increasing focused-ultrasound power and treatment time. Thus, focused ultrasound treatments were applied at 30 W and 100 W for 30 min in subsequent milk quality evaluation and compared with LTLT-treated milk. The IgG retention value of milk treated with focused ultrasound at 30 W and 100 W was higher than that of LTLT-treated milk (p &lt; 0.05). The alkaline phosphatase activity, pH, and whiteness index of milk treated with focused ultrasound at 100 W did not differ from LTLT-treated milk (p &gt; 0.05). Moreover, milk treated with focused ultrasound at 100 W exhibited the smallest particle size and fat globules (p &lt; 0.05). In conclusion, focused-ultrasound treatment at 55 °C and 100 W for 30 min improves milk microbial safety. Additionally, it preserves IgG activity and improves milk quality and stability by reducing fat globule size. Thus, focused ultrasound has the potential to become a novel milk pasteurisation technology for excellent antimicrobial effects and milk quality.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107615"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145399479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special issue on “Ultrasound-assisted separation processes”","authors":"Oualid Hamdaoui","doi":"10.1016/j.ultsonch.2025.107691","DOIUrl":"10.1016/j.ultsonch.2025.107691","url":null,"abstract":"","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107691"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145575324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Encapsulation of curcumin in groundnut oil-in-water (O/W) nanoemulsions: Experimental analysis and molecular dynamics simulations","authors":"Gerard Nathan Mathias ,&nbsp;K Vamshi Krishna Goud ,&nbsp;Srikanth Divi ,&nbsp;Anoop Kishore Vatti ,&nbsp;Jitendra Carpenter ,&nbsp;Sivakumar Manickam","doi":"10.1016/j.ultsonch.2025.107686","DOIUrl":"10.1016/j.ultsonch.2025.107686","url":null,"abstract":"<div><div>This study presents a comprehensive experimental and molecular simulation investigation aimed at developing highly stable and concentrated oil-in-water (O/W) nanoemulsions for curcumin encapsulation, using Tween 80 and Span 80 as emulsifiers. Experimentally, the influence of oil phase concentration (10–30 wt%) on key physicochemical parameters, including droplet size, polydispersity index (PDI), encapsulation efficiency, antioxidant activity, morphology, and long-term stability, was systematically investigated. All formulations exhibited excellent kinetic and oxidative stability during one month of storage, showing no significant changes in droplet size, size distribution, creaming index, or morphology. The encapsulation efficiency and antioxidant activity of curcumin remained consistently high and stable throughout the storage period. Complementing the experimental work, molecular dynamics (MD) simulations were conducted to elucidate the interactions of curcumin with fatty acids in the oil phase and with emulsifiers, thereby providing molecular-level insights into the nanoemulsion system’s structural organization and dynamic behavior. MD simulations confirmed strong molecular interactions between curcumin, oil components, and surfactants, validating the complete encapsulation of curcumin within the oil phase. Quantitative analyses of interaction energy, radius of gyration, and end-to-end distance further elucidated the stabilization mechanism and the cluster-formation behavior of curcumin in the emulsified system. Overall, the formulated emulsions demonstrated outstanding stability and bioactive retention, positioning them as highly effective carriers for curcumin delivery. These findings not only highlight the potential of the developed nanoemulsions for food and pharmaceutical applications but also provide critical molecular-level insights into their stabilization mechanisms, laying a strategic foundation for the design of next-generation functional delivery systems.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107686"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145575317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonic complexation with Lycium barbarum polysaccharide significantly enhances the aqueous solubility and bioavailability of curcumin","authors":"Li-Qiang Zhao ,&nbsp;Zhuo-Qiong Li ,&nbsp;Yue-Fan Liu ,&nbsp;Meng-Ting Jiang ,&nbsp;Ya-Nan Liu ,&nbsp;Xin-Lan Zhang ,&nbsp;Yu-Jie Sun ,&nbsp;Jia-Lun Duan ,&nbsp;Chun-Jie Bao ,&nbsp;Jin-Ao Duan","doi":"10.1016/j.ultsonch.2025.107673","DOIUrl":"10.1016/j.ultsonch.2025.107673","url":null,"abstract":"<div><div>Curcumin is a natural bioactive compound with a wide range of established health benefits. However, its practical applications are severely limited due to extremely poor aqueous solubility, which directly leads to low bioavailability. While polysaccharides like <em>Lycium barbarum</em> polysaccharide (LBP) can partially improve curcumin solubility, their solubilization efficiency remains limited. To overcome this challenge, we implemented ultrasonication as an effective processing strategy to enhance LBP’s capacity to promote curcumin dissolution. Our findings show that ultrasound-induced cavitation and related physico-chemical effects markedly improve LBP’s solubilization performance. The ultrasonically-assisted curcumin-LBP complex (CL-U) was systematically optimized through response surface methodology (RSM), identifying ultrasonic power, duration, and temperature as critical parameters. Extensive characterization verified that ultrasonication is essential for producing spherical core–shell nanoparticles, achieving a 2.23-fold enhancement in drug loading efficiency along with superior colloidal stability. Additional evidence from FTIR spectroscopy and acid hydrolysis experiments confirmed that ultrasonication reinforces hydrogen bonding as the principal intermolecular interaction stabilizing the complex. Biologically, CL-U demonstrated rapid cellular uptake in 4T1 cells within one hour and showed substantially improved antioxidant performance in both DPPH and ABTS assays. These functional gains are directly linked to the ultrasound-mediated improvements in solubility, stability, and bioavailability. This research establishes ultrasonication as a crucial sonochemical approach for constructing advanced polysaccharide-based delivery systems, providing a viable pathway for curcumin utilization in functional foods and pharmaceutical products.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107673"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonic-Assisted extraction of luteolin from peanut shells using supramolecular solvents and its molecular mechanism","authors":"Yuhan Fang ,&nbsp;Ping Zhang ,&nbsp;Shuang Wang ,&nbsp;Lulu Li ,&nbsp;Zunlai Sheng","doi":"10.1016/j.ultsonch.2025.107678","DOIUrl":"10.1016/j.ultsonch.2025.107678","url":null,"abstract":"<div><div>This study innovatively employs ultrasound-assisted extraction (UAE) in conjunction with supramolecular solvents (SUPRAS) for the extraction of luteolin from peanut shells, while also investigating the potential mechanism underlying this extraction process. Initially, the formation mechanism of SUPRAS was characterized using Fourier-transform infrared spectroscopy (FTIR). Based on the results of single-factor experiments, the extraction process was optimized using response surface methodology (RSM), resulting in the determination of optimal conditions: an ultrasound temperature of 67 °C, a SUPRAS/equilibrium solution (EqS) volume ratio of 5:1, and a solid-to-liquid ratio of 1:36 g/mL. Under these conditions, the maximum luteolin yield reached 1.645 mg/g. Furthermore, through independent gradient model (IGMH) calculations, molecular orbital theory, interaction energy analysis, and molecular dynamics simulations, it was revealed that the predominant interaction force between the supramolecular solvent and the target compound is hydrogen bonding. Notably, this study also compares the SUPRAS-UAE extraction method with the traditional extraction in terms of efficiency, economic costs, and environmental impact. The results indicate that the SUPRAS-UAE technique demonstrates significant advantages in terms of its green and high-efficiency characteristics. It provides a new sustainable strategy for the efficient recovery of natural active ingredients in chemical engineering and related fields.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107678"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145509821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic nanoblockers for synergistic cancer treatment through glutaminase inhibition and sonodynamic therapy","authors":"Wen Zhao ,&nbsp;Jintao Du ,&nbsp;Yunxuan Song ,&nbsp;Zilin Ma ,&nbsp;Songyan Li ,&nbsp;Yuhua Wei ,&nbsp;Guiqiang Zhang","doi":"10.1016/j.ultsonch.2025.107680","DOIUrl":"10.1016/j.ultsonch.2025.107680","url":null,"abstract":"<div><div>The immunotherapeutic potential of sonodynamic therapy (SDT) is hindered by tumor defense mechanisms driven by glutamine metabolism, including glutathione (GSH)-dependent redox homeostasis and an immunosuppressive tumor microenvironment (TME). To address these challenges, we herein developed metabolic nanoblockers (CBE@AM NPs) by encapsulating a glutamine metabolism inhibitor CB839 and a sonosensitizer chlorin e6 (Ce6) into melanin-inspired nanoparticles. The resulting nanoblockers triggered robust reactive oxygen species (ROS) production upon ultrasound irradiation, thereby destroying tumor cells and inducing immunogenic cell death (ICD). Concurrently, they inhibited the glutamine metabolism in the tumor, disrupting redox homeostasis and remodeling the immunosuppressive TME, thereby amplifying both SDT-generated oxidative stress and ICD-induced antitumor immunity. CBE@AM NPs demonstrated a potent tumor-inhibitory effect in tumor-bearing mice, highlighting their potential for immunometabolic reprogramming to enhance the therapeutic efficacy of SDT.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107680"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145531117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-stage ultrasound application for rice bran protein extraction: A novel method to improve yield, functional properties, and nutritional profile","authors":"Saydul Md Safwa,&nbsp;Nikitha Modupalli,&nbsp;Md Mahfuzur Rahman","doi":"10.1016/j.ultsonch.2025.107681","DOIUrl":"10.1016/j.ultsonch.2025.107681","url":null,"abstract":"<div><div>While ultrasound-assisted protein extraction is well-studied, the application of ultrasound in both the extraction and precipitation stages has not been explored. This study explored the ultrasound (U)-assisted alkaline extraction (AE) at pH 9.5 and ultrasound-assisted isoelectric precipitation (UIP) at pH 3.5 on the rice bran protein yield, functionality, and nutritional quality. The UAE and conventional precipitation (UAE-IP) yielded the highest protein yield of 65.7 %, while conventional extraction yielded 38.9 %. FTIR showed that UAE-IP increased β-sheets by 96.8 % while AE-UIP decreased the α-helices by 42.4 %. Although UAE-IP showed no significant change in surface hydrophobicity, UAE-UIP decreased it by 34.7 % to the control. Consequently, functional properties such as solubility, emulsifying, and foaming capacities were significantly improved by 5 %, 0.05, and 3 % respectively. AE-UIP exhibited the highest solubility at pH 7, while UAE-IP showed better solubility at acidic and alkaline pHs. UAE-UIP demonstrated superior emulsion and foaming properties. Ultrasound application enhanced protein digestibility, with AE-UIP showing the highest digestibility of 96.91 %. However, dual-stage ultrasound reduced protein digestibility and subsequently affected the amino acid score (AAS). The amino acid profile varied with extraction methods, with AE-IP showing the highest levels of essential amino acids. AE-IP and UAE-IP showed no significant difference in PDCAAS (protein digestibility–corrected amino acid score) and AAS, whereas AE-UIP and UAE-UIP reduced them to 0.9 and 0.6, respectively. In summary, while ultrasound-assisted extraction enhances protein yield and certain properties, ultrasound-assisted precipitation shows great promise in further enhancing mass yield and functionality, which underscores the importance of a dual-stage ultrasound.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107681"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145531115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}