Ultrasonics Sonochemistry最新文献

{"title":"Bubble shape instability of acoustic cavitation in molten metal used in ultrasonic casting","authors":"Takuya Yamamoto","doi":"10.1016/j.ultsonch.2024.107064","DOIUrl":"10.1016/j.ultsonch.2024.107064","url":null,"abstract":"<div><p>In this study, we estimated the equilibrium bubble size of acoustic cavitation in a molten metal, which is basic information in ultrasonic casting. For this, the bubble shape instability of acoustic cavitation in the melt was numerically investigated by solving the Keller–Miksis equation and dynamic equation of the distortion amplitude. The acoustic cavitation bubbles are more stable in aluminum and magnesium melts than in water, and the <em>parametric instability</em> mainly determines the bubble stability at 20–160 kHz in molten metals. However, the <em>afterbounce instability</em> does not significantly affect the bubble stability in molten metals owing to the small number of bubble oscillations after the first rapid compression, and the distortion amplitude cannot grow significantly after the first compression. The bubbles in the melt become more unstable with an increase in the ultrasonic frequency owing to the corresponding increase in the bubble wall velocity. Through this stability analysis, we can estimate that the stable bubble size in the aluminum or magnesium melt is approximately three or four times larger than that in water at the same ultrasonic pressure amplitude.</p></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107064"},"PeriodicalIF":8.7,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003122/pdfft?md5=62e4e88425966b2b76259c65bfffe671&pid=1-s2.0-S1350417724003122-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of distribution parameters on acoustic radiation from bubble clusters","authors":"Fuqiang Deng,&nbsp;Lingxin Zhang,&nbsp;Peng Wang,&nbsp;Yizhe Wu,&nbsp;Di Zhao,&nbsp;Yang Li","doi":"10.1016/j.ultsonch.2024.107066","DOIUrl":"10.1016/j.ultsonch.2024.107066","url":null,"abstract":"<div><p>Cavitation noise is the major noise in underwater, and the study of acoustic radiation from bubble clusters is the primary means to reveal the mechanism of cavitation noise. In this study, direct numerical simulation (DNS) of bubble clusters with volume fractions of 20–40 % with different bubble sizes and bubble position distributions are performed, and the far-field sound pressure is calculated using the Ffowcs Williams–Hawkings (FW-H) method. Then, we compare the collapse and acoustic radiation of bubble clusters with equivalent bubble. The results show that the collapse times of bubble clusters at the same volume fraction are identical and close to equivalent bubble, despite the different bubble sizes and positions in the bubble cluster. Further, in terms of acoustic radiation, the layered arrangement of bubble positions results in bubble clusters exhibiting layer-by-layer collapse and emitting multiple sound pressure pulses. In contrast, a random arrangement of bubble positions lacks this feature, resulting in the collapse of the bubble cluster without a layered phenomenon and radiating only a single primary sound pulse, which is consistent with the equivalent bubble. Additionally, the distribution of bubble sizes in the bubble cluster has almost no effect on the acoustic radiation of the bubble cluster. Notably, when the volumetric fraction exceeds 25 %, the sound pressure levels of bubble clusters with different distributions in the frequency domain are nearly identical, with differences from the equivalent bubble within 5 dB.</p></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107066"},"PeriodicalIF":8.7,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003146/pdfft?md5=147b63ca6a1f1e98c588236547de8cc7&pid=1-s2.0-S1350417724003146-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142241866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dissolution of EAF slag minerals in aqueous media: Effects of sonication on brownmillerite and gehlenite","authors":"Recep Kurtulus ,&nbsp;Mahtab Akbarzadeh Khoei ,&nbsp;Elijah Damilola Adesanya ,&nbsp;Juho Yliniemi","doi":"10.1016/j.ultsonch.2024.107065","DOIUrl":"10.1016/j.ultsonch.2024.107065","url":null,"abstract":"<div><p>The accumulation of electric arc furnace slag (EAFS) in landfills has been causing severe environmental problems. This study examines the dissolution properties of EAFS minerals, including brownmillerite and gehlenite, essential for their possible use in resource recovery. An investigation was conducted to compare the effects of sonication and stirring on mineral dissolution while also assessing the usage of citrate as a complexing agent for gehlenite. Synthetic brownmillerite and gehlenite minerals were dissolved in aqueous solutions at room temperature using a 1:100 g/ml ratio. The dissolved elements were measured using Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES), while zeta potential and X-ray Photoelectron Spectroscopy (XPS) were used to assess changes in surface chemistry. Brownmillerite had significant dissolution extents, with Al and Ca dissolving up to 16 % and 8 %, respectively, in contrast to gehlenite, which dissolved less than 2 % under similar conditions. Sonication significantly increased the dissolution of brownmillerite by up to 100 %, although its impact on gehlenite dissolution varied depending on the duration of time. Besides, adding citrate enhanced the leaching of Al and Ca from gehlenite by facilitating complexation. XPS data demonstrated differences in elemental ratios on brownmillerite and gehlenite surfaces affected by the method used and the presence of citrate. Lastly, the dissolution extents of Al and Ca from EAFS were up to 12 %, depending on time and mixing method, with a preference for sonication over stirring. In conclusion, this study showed that minerals in EAFS have distinct dissolution characteristics, and sonication and citrate can considerably enhance dissolution.</p></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"110 ","pages":"Article 107065"},"PeriodicalIF":8.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003134/pdfft?md5=64eb000729a885a9907c6407e8f7bb6a&pid=1-s2.0-S1350417724003134-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142173351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application and development of ultrasound in industrial crystallization","authors":"Liuxin Xiang ,&nbsp;Mingge Fu ,&nbsp;Tian Wang ,&nbsp;Dongbin Wang ,&nbsp;Haoran Xv ,&nbsp;Wenlong Miao ,&nbsp;Thiquynhxuan Le ,&nbsp;Libo Zhang ,&nbsp;Jue Hu","doi":"10.1016/j.ultsonch.2024.107062","DOIUrl":"10.1016/j.ultsonch.2024.107062","url":null,"abstract":"<div><p>Crystallization is an important process that affects the properties of final products and is essential in nearly all chemical processing industries. In recent years, ultrasonic technology has received widespread attention due to its ability to enhance crystallization yield, improve crystal morphology and shape, and regulate the particle size and distribution of crystal products. It holds promising prospects for industrial crystallization. In this work, the ultrasonic cavitation effect and ultrasonic crystallization mechanism are described, and the influence of ultrasound on the crystallization effect of products is analysed and discussed. In addition, the application status of ultrasonic reactors and ultrasonic crystallization processes is introduced in detail, and the change trend from laboratory to industrialization is analyzed. Finally, the challenges and opportunities facing the industrialization of ultrasonic crystallization in future developments are discussed. The purpose of this work is to make the selective promotion or inhibition of ultrasound more helpful for industrial crystallization.</p></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107062"},"PeriodicalIF":8.7,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003109/pdfft?md5=e5210c7560659cd6e29f7359dfd8ebaf&pid=1-s2.0-S1350417724003109-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142241867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Numerical investigation of acoustic cavitation characteristics of a single gas–vapor bubble in soft tissue under dual-frequency ultrasound","authors":"Zhenxiang Ji ,&nbsp;Dingjie Suo ,&nbsp;Jie Jin ,&nbsp;Xinze Liu ,&nbsp;Ye Wang ,&nbsp;Shintaro Funahashi ,&nbsp;Wei Li ,&nbsp;Tianyi Yan","doi":"10.1016/j.ultsonch.2024.107061","DOIUrl":"10.1016/j.ultsonch.2024.107061","url":null,"abstract":"<div><div>The viscoelastic tissue under dual-frequency ultrasound excitation affects the acoustic cavitation of a single gas–vapor bubble. To investigate the effect of the cavitation dynamics, the Gilmore-Akulichev-Zener (GAZ) model is coupled with the Peng-Robinson equation of state (PR EOS). Results indicate that the GAZ-PR EOS model can accurately estimate the bubble dynamics by comparing with the Gilmore PR EOS and GAZ-Van der Waals (VDW) EOS model. Furthermore, the acoustic cavitation effect in different viscoelastic tissues is investigated, including the radial stress at the bubble wall, the temperature, pressure, and the number of water molecules inside the bubble. Results show that the creep recovery and the relaxation of the stress caused by viscoelasticity can affect the acoustic cavitation of the bubble, which could inhibit the bubble’s expansion and reduce the internal temperature and pressure within the bubble. Moreover, the effect of dual-frequency ultrasound on the cavitation of single gas–vapor bubbles is studied. Results suggest that dual-frequency ultrasound could increase the internal temperature of bubbles, the internal pressure of bubbles, and the radial stress at the bubble wall. More importantly, there is a specific optimal combination of frequencies for particular viscoelasticity by exploring the impact of different dual-frequency ultrasound combinations and tissue viscoelasticity on the acoustic cavitation of a single gas–vapor bubble. In conclusion, this study helps to provide theoretical guidance for dual-frequency ultrasound to improve acoustic chemical and mechanical effects, and further optimize its application in acoustic sonochemistry and ultrasound therapy.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107061"},"PeriodicalIF":8.7,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003092/pdfft?md5=cf376dc33385661f4a3bc2b4e466227d&pid=1-s2.0-S1350417724003092-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental study on attenuation effect of liquid viscosity on shockwaves of cavitation bubbles collapse","authors":"Jing Luo ,&nbsp;Guihua Fu ,&nbsp;Weilin Xu ,&nbsp;Yanwei Zhai ,&nbsp;Lixin Bai ,&nbsp;Jie Li ,&nbsp;Tong Qu","doi":"10.1016/j.ultsonch.2024.107063","DOIUrl":"10.1016/j.ultsonch.2024.107063","url":null,"abstract":"<div><p>How to precisely control and efficiently utilize the physical processes such as high temperature, high pressure, and shockwaves during the collapse of cavitation bubbles is a focal concern in the field of cavitation applications. The viscosity change of the liquid will affect the bubble dynamics in turn, and further affect the precise control of intensity of cavitation field. This study used high-speed photography technology and schlieren optical path system to observe the spatiotemporal evolution of shockwaves in liquid with different viscosities. It was found that as the viscosity of the liquid increased, the wave front of the collapse shockwave of the cavitation bubble gradually thickened. Furthermore, a high-frequency pressure testing system was used to quantitatively analyze the influence of viscosity on the intensity of the shockwave. It was found that the pressure peak of the shockwave in different viscous liquid was proportional to <em>L</em>^b (<em>L</em> represented the distance between the center of bubble and the sensor measuring point), and the larger the viscosity was, the smaller the value of <em>b</em> was. Through in-depth analysis, it was found that as the viscosity of the liquid increased, the proportion of the shockwave energy of first bubble collapse to the maximal mechanical energy of bubble gradually decreased. The proportion of the mechanical energy of rebounding bubble to the maximal mechanical energy of bubble gradually increased. These new findings have an important theoretical significance for the efficient utilization of ultrasonic cavitation.</p></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107063"},"PeriodicalIF":8.7,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003110/pdfft?md5=7b031f3b8e915fa77fd5e66bb628d24e&pid=1-s2.0-S1350417724003110-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142241865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into the effects of ultrasound-assisted intermittent tumbling on the gelation properties of myofibrillar proteins: Conformational modifications, intermolecular interactions, rheological properties and microstructure","authors":"Ruyu Zhang,&nbsp;Lei Zhou,&nbsp;Wangang Zhang","doi":"10.1016/j.ultsonch.2024.107059","DOIUrl":"10.1016/j.ultsonch.2024.107059","url":null,"abstract":"<div><p>The aim of the present study was to evaluate the effects of ultrasound-assisted intermittent tumbling (UT) at 300 W, 20 kHz and 40 min on the conformation, intermolecular interactions and aggregation of myofibrillar proteins (MPs) and its induced gelation properties at various tumbling times (4 and 6 h). Raman results showed that all tumbling treatments led the helical structure of MPs to unfold. In comparison to the single intermittent tumbling treatment (ST), UT treatment exerted more pronounced effects on strengthening the intermolecular hydrogen bonds and facilitating the formation of an ordered β-sheet structure. When the tumbling time was the same, UT treatment caused higher surface hydrophobicity, fluorescence intensity and disulfide bond content in the MPs, inducing the occurrence of hydrophobic interaction and disulfide cross-linking between MPs molecules, thus forming the MPs aggregates. Additionally, results from the solubility, particle size, atomic force microscopy and SDS-PAGE further indicated that, relative to the ST treatment, UT treatment was more potent in promoting the polymerization of myosin heavy chain. The MPs aggregates in the UT group were more uniform than those in the ST group. During the gelation process, the pre-formed MPs aggregates in the UT treatment increased the thermal stability of myosin, rendering it more resistant to heat-induced unfolding of the myosin rod region. Furthermore, they improved the protein tail–tail interaction, resulting in the formation of a well-structured gel network with higher gel strength and cooking yield compared to the ST treatment.</p></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"110 ","pages":"Article 107059"},"PeriodicalIF":8.7,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003079/pdfft?md5=6aa781ff3a0f1232f2c4d90bd356ef85&pid=1-s2.0-S1350417724003079-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142157765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The improvement mechanism of volatile for cooked Tibetan pork assisted with ultrasound at low-temperature: Based on the differences in oxidation of lipid and protein","authors":"Lujie Cheng ,&nbsp;Xin Li ,&nbsp;Xiefei Li ,&nbsp;Yingmei Wu ,&nbsp;Fengping An ,&nbsp;Zhang Luo ,&nbsp;Fang Geng ,&nbsp;Qun Huang ,&nbsp;Zhendong Liu ,&nbsp;Yuting Tian","doi":"10.1016/j.ultsonch.2024.107060","DOIUrl":"10.1016/j.ultsonch.2024.107060","url":null,"abstract":"<div><p>Low-temperature cooking causes flavor weakness while improving the texture and digestive properties of meat. To enhance the flavor of low-temperature cooked Tibetan pork, samples were cooked at low-temperature with or without ultrasound-assisted (UBTP, BTP) for different times (30 min, 90 min) and then analyzed using GC–MS and LC-MS. The results showed that ultrasound-assisted cooking caused a significant increase in lipid oxidation by 9.10% in the early stage of the treatment. Additionally, at the later stage of ultrasound-assisted processing, proteins were oxidized and degraded, which resulted in a remarkable rise in the protein carbonyl content by 6.84%. With prolonged effects of ultrasound and low-temperature cooking, the formation of phenylacetaldehyde in UBTP-90 sample originated from the degradation of phenylalanine through multivariate statistics and correlation analysis. Meanwhile, trans, cis-2,6-nonadienal and 1-octen-3-one originated from the degradation of linolenic acid and arachidonic acid. This study clarified the mechanism of ultrasound-assisted treatment improving the flavor of low-temperature-cooked Tibetan pork based on the perspective of lipids and proteins oxidation, providing theoretical supports for flavor enhancement in Tibetan pork-related products.</p></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"110 ","pages":"Article 107060"},"PeriodicalIF":8.7,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003080/pdfft?md5=931e4e7486324c861c70dd84b983bf7b&pid=1-s2.0-S1350417724003080-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142161725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial regulation of hydrogel polymerization reaction using ultrasound-driven streaming vortex","authors":"Byungjun Kang ,&nbsp;Jisoo Shin ,&nbsp;Donyoung Kang ,&nbsp;Sooho Chang ,&nbsp;Chanryeol Rhyou ,&nbsp;Seung-Woo Cho ,&nbsp;Hyungsuk Lee","doi":"10.1016/j.ultsonch.2024.107053","DOIUrl":"10.1016/j.ultsonch.2024.107053","url":null,"abstract":"<div><p>Ultrasound is gaining attention as an alternative tool to regulate chemical processes due to its advantages such as high cost-effectiveness, rapid response, and contact-free operation. Previous studies have demonstrated that acoustic bubble cavitation can generate energy to synthesize functional materials. In this study, we introduce a method to control the spatial distribution of physical and chemical properties of hydrogels by using an ultrasound-mediated particle manipulation technique. We developed a surface acoustic wave device that can localize micro-hydrogel particles, which are formed during gelation, in a hydrogel solution. The hydrogel fabricated with the application of surface acoustic waves exhibited gradients in mechanical, mass transport, and structural properties. We demonstrated that the gel having the property gradients could be utilized as a cell-culture substrate dictating cellular shapes, which is beneficial for interfacial tissue engineering. The acoustic method and fabricated hydrogels with property gradients can be applied to design flexible polymeric materials for soft robotics, biomedical sensors, or bioelectronics applications.</p></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"110 ","pages":"Article 107053"},"PeriodicalIF":8.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003018/pdfft?md5=5d73f2442c226271c0be3b3fa3f99e75&pid=1-s2.0-S1350417724003018-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modifying physicochemical properties, rheology, and creaming stability of milk fat globule and membrane through ultrasound treatment","authors":"Yanjun Sun ,&nbsp;Yrjö H. Roos ,&nbsp;Song Miao","doi":"10.1016/j.ultsonch.2024.107058","DOIUrl":"10.1016/j.ultsonch.2024.107058","url":null,"abstract":"<div><p>The healthy benefits of milk fat globules and membrane (MFGs/MFGM) ingredients are increasingly recognized in the dairy industry. In this research, we examined the effects of ultrasonic treatment on the physicochemical and rheological properties, as well as the emulsions stability of MFGs/MFGM derived from bovine raw milk. Fresh milk was subjected to sonication at frequencies of 20 kHz and 40 kHz, either individually or simultaneously, for durations of 5 min or 15 min, using work/rest cycles of 5 s on and 3 s off. Bovine milk, without any treatment, served as the control. Regardless of the intensity difference, ultrasonic treatment for 5 min resulted in more pronounced changes in the regions of Amide Ⅱ (1600–1500 cm⁻¹), Amide Ⅲ (1500–1200 cm⁻¹), and fingerprint region (1200–1900 cm⁻¹) compared to both the 15 min treatments and control MFGs/MFGM. Principal component analysis (PCA) conducted on the entire spectra, as well as in the regions of Amide Ⅰ, Amide Ⅱ, and the fingerprint spectra, clustered the 5 min treatment distinctly from the control and MFGs/MFGM ultrasonically treated for 15 min. MFGs/MFGM samples following 20 kHz and 40 kHz synchronous treatment for 15 min exhibited lower absorbance bands at 1727–1726 cm⁻¹, whereas a higher content at 1740 cm⁻¹ was observed compared to control MFGs/MFGM. Additionally, a more significant reduction in the intramolecular β-sheet content in 20 + 40 kHz/ 15 min treatment was observed. According to the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) patterns, a diminished intensity of Periodic Acid Schiff 6/7 (PAS 6/7) bands was observed across all the MFGs/MFGM. Ultrasonic treatment retained more caseins while reducing the β –LG levels compared to the controls, enhancing the stability of MFGs/MFGM, except in MFGs/ MFGM subjected to 20 and 40 kHz simultaneously treated for 15 min. The irregular sphericity of fat globules was noted particularly in MFGs/MFGM treated at 20 kHz independently or in combination with 40 kHz for 15 min. According to the confocal laser scanning microscopy (CLSM), ultrasonic treatment facilitated the binding of caseins or whey proteins to the MFGs surface and induced flocculation of membrane proteins. Hierarchical cluster analysis (HCA) heat map further underscored the impact of ultrasonic treatments on the structural and compositional changes, as well as rheology and emulsions stability, of MFGs/MFGM.</p></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107058"},"PeriodicalIF":8.7,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003067/pdfft?md5=526cb44d388d49885c7d5d5d93ca4038&pid=1-s2.0-S1350417724003067-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}