Ultrasonics Sonochemistry最新文献

{"title":"Rapid and efficient high-performance liquid chromatography-ultraviolet determination of total amino acids in protein isolates by ultrasound-assisted acid hydrolysis","authors":"Jorge A. Custodio-Mendoza,&nbsp;Patryk Pokorski,&nbsp;Havva Aktaş,&nbsp;Marcin A. Kurek","doi":"10.1016/j.ultsonch.2024.107082","DOIUrl":"10.1016/j.ultsonch.2024.107082","url":null,"abstract":"<div><div>This study presents the optimization and validation of an ultrasound-assisted acid method for the HPLC-UV determination of amino acids in plant-based proteins. The research focuses on enhancing hydrolysis efficiency and reducing environmental impact. Ultrasound treatment significantly accelerated hydrolysis by creating cavitation, which increases local pressure and temperature, leading to faster reaction rates. The optimal condition was a 30-minute treatment at 90 °C with 6 M hydrochloric acid. The 9-fluorenylmethyloxycarbonyl chloride derivatization was best performed at pH 9.0 using borate buffer, ethanol as the organic solvent, and a 5-minute derivatization time with a 5 mM concentration. The method’s analytical performance, validated according to FDA guidelines, showed excellent selectivity, specificity, linearity (r² &gt; 0.999), accuracy (recovery between 80–118 %), and precision (RSD&lt;10.9). The analysis of 15 plant-based proteins revealed distinct amino acid profiles. Compared to traditional acid hydrolysis methods, the ultrasound-assisted approach demonstrated no significant difference in results (p-value &gt; 0.05), confirming its reliability. The optimized ultrasound-assisted method is a reliable and efficient alternative for amino acid analysis, offering significant cost and time savings while maintaining high analytical performance. These findings are crucial for nutritional planning and developing functional foods to improve health outcomes.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107082"},"PeriodicalIF":8.7,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound combined with FeSO4 facilitated the occurrence of ferroptosis in Vibrio parahaemolyticus","authors":"Shurui Peng ,&nbsp;Lishan Yao ,&nbsp;Xiaolin Zhu,&nbsp;Wei Ge,&nbsp;Jiakun Deng,&nbsp;Hongbo Li,&nbsp;Dan Xu,&nbsp;Liangbin Hu,&nbsp;Haizhen Mo","doi":"10.1016/j.ultsonch.2024.107080","DOIUrl":"10.1016/j.ultsonch.2024.107080","url":null,"abstract":"<div><div>Ultrasound (US) as a sustainable non-thermal sterilization technology that is employed either independently alone or in combination with other processing methods to eliminate food-borne pathogens in the food industry. In the present study, the synergistic effects of US combined with FeSO₄ against <em>Vibrio parahaemolyticus</em> were investigated. The results demonstrated that the combination of ultrasound and FeSO₄ had an excellent bactericidal activity on <em>V. parahaemolyticus</em>. Treatment with US (100 W) and FeSO₄ (8 μM) for 15 min could kill more than 99.9 % cells. Furthermore, the observed cell death was identified as classical ferroptosis, characterized by ferroptosis hallmarks including iron-dependent, ROS burst, membrane damage and lipid peroxide accumulation. Addition of ferroptosis inhibitor liproxstatin-1 alleviated the cell death induced by the combination treatment. Transcriptome analysis further revealed that the US-FeSO₄ treatment significantly influenced pathways related to fatty acid metabolism, ferroptosis, biofilm formation, RNA degradation, oxidative phosphorylation and other key processes, which likely contributed to the occurrence of ferroptosis. Based on these findings, we speculated that cavitation effect of US promoted the entry of Fe²⁺, leading to the generation of free radicals primarily responsible for ferroptosis by US-FeSO₄. Taken together, this study provides valuable insights into the biological pathway involved in ultrasound sterilization and presents an alternative strategy to eradicate microorganism in food products.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107080"},"PeriodicalIF":8.7,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003286/pdfft?md5=8ae42c83c555e257fcd66ba4520d3131&pid=1-s2.0-S1350417724003286-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted extraction, optimization, and purification of total flavonoids from Daphnegenkwa and analysis of their antioxidant, anti-inflammatory, and analgesic activities","authors":"Yi Xiang ,&nbsp;Zheng Liu ,&nbsp;Yanzhi Liu ,&nbsp;Bin Dong ,&nbsp;Changqing Yang ,&nbsp;Hanhan Li","doi":"10.1016/j.ultsonch.2024.107079","DOIUrl":"10.1016/j.ultsonch.2024.107079","url":null,"abstract":"<div><div><em>Daphne genkwa (D. genkwa)</em> is the dried flower buds of a Chinese medicinal plant with multiple biological activities. Response surface methodology (RSM) combined with artificial neural network (ANN) techniques were utilized to optimize ultrasound-assisted extraction conditions for <em>D. genkwa</em>. Antioxidant activity and anti-inflammatory and analgesic properties of total flavonoids from <em>D. genkwa</em> (TFDG) were assessed. Optimal conditions involving ultrasonic power of 225 W, 30 min extraction time, 30 mL/g liquid–solid ratio, 60 °C extraction temperature, and 70% ethanol concentration yielded a maximum total flavonoids content (TFC) of 5.41 mg/g. After microporous resin purification, four specific flavonoids in <em>D. genkwa</em> were identified and quantified using high-performance liquid chromatography (HPLC). The TFDG demonstrated potent antioxidant activity, with a 94% rate of scavenging the 2, 2-diphenyl-1-picrylhydrazyl (DPPH). Furthermore, TFDG exhibited pain-alleviating properties in hot plate and acetic acid-induced writhing tests and noteworthy inhibitory effects on xylene-induced ear swelling in mice. The total flavonoids extracted by ultrasound had excellent biological activity. This establishes a foundation for further investigation into the potential medical value of <em>D. genkwa</em>.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107079"},"PeriodicalIF":8.7,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142329795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of inorganic salts on the ultrasonic degradation of contaminants: A review","authors":"Haleigh A. Fernandez,&nbsp;Linda K. Weavers","doi":"10.1016/j.ultsonch.2024.107076","DOIUrl":"10.1016/j.ultsonch.2024.107076","url":null,"abstract":"<div><div>This comprehensive review explores the interplay between inorganic salts and ultrasound-assisted degradation of various contaminants. The addition of salt to aqueous matrices has been attributed to increasing contaminant degradation via the salting-out effect. However, research investigating the impact of salt on degradation has yielded inconsistent results. This review incorporated degradation information from 44 studies organizing data according to compound class and ionic strength to analyze the impact of inorganic salts on cavitation bubble dynamics, contaminant behavior, radical species generation, and contaminant degradation. Frequency and salt type were assessed for potential roles in contaminant degradation. The analysis showed that high intensity ultrasound was most beneficial to degradation in salt solutions. Unexpectedly, hydrophilic compounds showed marked enhancement with increasing ionic strength while many hydrophobic compounds did not benefit as greatly. Based on the collected data and analysis, enhanced degradation in the presence of salt appears to be primarily radical-mediated rather than due to the salting-out effect. Finally, the analysis provides guidance for designing sonolytic reactors for contaminant degradation.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107076"},"PeriodicalIF":8.7,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142363888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Focused shock waves and inertial cavitation release tumor-associated antigens from renal cell carcinoma","authors":"Nushin Hosano ,&nbsp;Zahra Moosavi-Nejad ,&nbsp;Takuichiro Hide ,&nbsp;Hamid Hosano","doi":"10.1016/j.ultsonch.2024.107078","DOIUrl":"10.1016/j.ultsonch.2024.107078","url":null,"abstract":"<div><div>Tumor biomarkers play an essential role in immunotherapeutic strategies in cancer treatment, contributing to early diagnosis, patient selection, treatment monitoring, and personalized treatment plans. Despite their importance in cancer care, circulating biomarkers may not always be detectable or sufficiently elevated to provide reliable test results. Due to the pressing need for innovative approaches to enhance biomarker levels, this study explored the potential use of focused shock waves and cavitation for non-invasively releasing tumor-associated antigens. Renal carcinoma cell lines ACHN and TOS-1 were used in an <em>in vitro</em> study to analyze the impact of shock waves on two membrane glycosphingolipid antigens, MSGG and G1, respectively. Focused shock waves were generated using a partial spherical piezoceramic dish. Flow-cytometric analysis of treated cells immediately after 1,000 focused shock waves at 16 MPa overpressure showed a 29.4 % and 17.6 % decrease in MSGG and G1 antigens on the cell surfaces. In the immunostaining of glycosphingolipid fractions on thin-layer chromatography (TLC), both tumor markers were reduced by an average of 49.30 % (MSGG) and 57.08 % (G1). Immunoelectron microscopy images confirmed decrease in the cell membrane intensity immediately after shock waves because of the release of antigens into the extracellular spaces. The released antigens were primarily found on cell debris formed by shock waves and cavitation induced damage to the cell membrane. Theoretical analyses were performed to understand antigen release mechanisms. Moreover, the biophysical events that occurred following the interaction of a shock wave with a suspended cell were modeled and clarified. A novel model was used to calculate the tensile stresses following shock waves and to explain the deformations observed in scanning electron microscopy images. The release of tumor antigens by focused shock waves and inertial cavitation represents exciting prospects for advancing cancer care strategies.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107078"},"PeriodicalIF":8.7,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142338677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the formation and characteristics of unstable sheet cavitation on hydrofoils","authors":"Lin Wang ,&nbsp;Di Chen ,&nbsp;Fangping Tang ,&nbsp;Haiyu Liu","doi":"10.1016/j.ultsonch.2024.107074","DOIUrl":"10.1016/j.ultsonch.2024.107074","url":null,"abstract":"<div><div>Hydrofoils, as fundamental components of hydraulic machinery, directly influence the performance of such machinery. This study conducted an analysis of the cavitation characteristics of hydrofoils at a + 4° angle of attack under various cavitation numbers using numerical simulation and experimental research methods. The focus of the research was to explore the phenomenon of unstable sheet cavitation and its causes, as well as to reveal the characteristics of the re-entrant jet. The large eddy simulation method was employed to calculate the cavitation morphology under three different cavitation numbers. The method is highly consistent with the experimental results in simulating the small-scale detachment at the tail of unstable sheet cavitation and the large-scale shedding of cloud cavitation. The study found that the detachment of unstable sheet cavitation is closely related to the re-entrant jet, which exhibits transient and abrupt characteristics during the unstable sheet cavitation phase. Furthermore, by applying FFT processing to the distribution of maximum reverse velocity and the spatiotemporal changes of <em>Ux</em> on characteristic lines, eigenfrequency of the detachment of unstable sheet cavitation were identified. The research results indicate that cavitation mainly show as sheet cavitation when the cavitation closure point does not exceed the zero-slope point. Beyond this point, it transitions to cloud cavitation. This study provides new insights into the cavitation phenomenon of hydrofoils and offers quantitative research on the phenomenon of unstable sheet cavitation.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107074"},"PeriodicalIF":8.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003225/pdfft?md5=966b9755a5928c3e7b3fda234dde1d4d&pid=1-s2.0-S1350417724003225-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142275695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compressible effect on the mutual interaction of two cavitation bubbles in radial oscillations and translational motion","authors":"Hancheng Wang,&nbsp;Junjie Jiao,&nbsp;Xuchao Pan,&nbsp;Yanjie Qi,&nbsp;Feng Shan,&nbsp;Zhong Fang,&nbsp;Chuanting Wang,&nbsp;Yong He","doi":"10.1016/j.ultsonch.2024.107075","DOIUrl":"10.1016/j.ultsonch.2024.107075","url":null,"abstract":"<div><div>In this study, we considered the compressible effect on the mutual interaction of two cavitation bubbles by correcting the sound field emitted by one bubble in the radial equations of the other bubble to first order in the Mach number of the flow, and the effect is represented by the incident wave acting on bubbles. The results illustrates that the incident wave can enhance the resonance response at the redistributed resonance frequency, which leads to an increase in radial acceleration and the secondary Bjerknes force, and rapid approach of bubbles. Furthermore, the influence of incident wave on the interaction of bubbles driven at lower frequencies is more significant, due to resonance enhancement caused by the proximity of natural frequencies and frequency multiplications of the external sound field. Our findings reveal that the compressible effect is not only critical to interaction in radial oscillations, but also in translational motion.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107075"},"PeriodicalIF":8.7,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003237/pdfft?md5=b98a0ff5fe03085cd529543ec4b0b63f&pid=1-s2.0-S1350417724003237-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142275696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable ultrasonic extraction of antibacterial Basella alba fruit dye for cotton, silk, and leather","authors":"Loganathan Lingeshwaran ,&nbsp;Jagadeesh Kumar Alagarasan ,&nbsp;Seema siddharthan ,&nbsp;Kanagasabapathy Sivasubramanian ,&nbsp;Palanivel Velmurugan ,&nbsp;Fatimah Oleyan Al-Otibi ,&nbsp;Sivakumar Manickam ,&nbsp;Moonyong Lee","doi":"10.1016/j.ultsonch.2024.107069","DOIUrl":"10.1016/j.ultsonch.2024.107069","url":null,"abstract":"<div><div>Alternative to synthetic dyes containing harmful compounds, dyes derived from natural sources are gaining popularity due to their safer and eco-friendly nature. This study focuses on extracting red dye from <em>Basella alba</em> fruit and optimising the extraction methods, including ultrasonic bath, ultrasonic probe, and direct heating. The extracted dye was then used for dyeing cotton, silk, and leather without needing a mordant. Furthermore, the antibacterial properties of the extracted red dye were evaluated against skin bacteria. The UV–Visible spectrophotometric analysis revealed that the maximum red colour in the methanol extract (λ_max 270 and λ_max 542 nm) was achieved at 60 °C for 30 min using the ultrasonic water bath extraction method, followed by the ultrasonic probe and direct heating methods. The FTIR spectra confirmed the presence of flavonoids, betacyanin, and gomphrenin-I in the extracted dye. The ultrasonic dyeing process at 50 °C yielded a K/s value 6.3 for the dyed cotton, silk, and leather without using a mordant. Additionally, the fatness test indicated a high grade of 0.5–1.5 for the ultrasonic dyeing method compared to other dyeing techniques. The extracted dye exhibited significant antibacterial activity against all <em>Pseudomonas</em> sp. after extraction in methanol, with the highest inhibition observed against <em>Pseudomonas</em> sp. with a MIC of 1.56 mg/ml.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107069"},"PeriodicalIF":8.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003171/pdfft?md5=e278f52a814780029d383ac6180b6df7&pid=1-s2.0-S1350417724003171-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142275709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of zein-based films for mango preservation using high-intensity ultrasound and castor oil plasticization","authors":"Xin Fan,&nbsp;Lu Chang,&nbsp;Huayin Pu,&nbsp;Jinghua Zhao,&nbsp;Huan Wang,&nbsp;Yiyu Wang,&nbsp;Wenqiang He,&nbsp;JunRong Huang","doi":"10.1016/j.ultsonch.2024.107067","DOIUrl":"10.1016/j.ultsonch.2024.107067","url":null,"abstract":"<div><p>Zein-based films exhibit high efficiency in ethylene adsorption. However, its brittleness limits the practical applications. To address this issue, this study synergizes the plasticizing effects of high-intensity ultrasound (HIU) and castor oil (CO) to reduce the brittleness of zein-based films. The plasticizing mechanism was demonstrated through the formation of new intermolecular hydrogen bonds and electrostatic interactions, as evidenced by fourier transform infrared spectroscopy (FTIR) and zeta potential measurements. The tensile strength of 6 % CO-zein film increased eightfold. Additionally, the freshness of mangoes stored with 6 % CO-zein film significantly improved, extending their shelf life from 5 days to 15 days. Therefore, this study investigated the synergistic plasticization of zein-based films through the addition of CO, based on HIU. It also provides a theoretical basis for fruit packaging.</p></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107067"},"PeriodicalIF":8.7,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003158/pdfft?md5=24e3cdc80a08e9a7bb7ee76a4dd66c3a&pid=1-s2.0-S1350417724003158-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142241864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonic field-assisted metal additive manufacturing (U-FAAM): Mechanisms, research and future directions","authors":"Xuekai Li ,&nbsp;Wei Wang ,&nbsp;Yihong Wu ,&nbsp;Donghu Zhou ,&nbsp;Huijun Kang ,&nbsp;Enyu Guo ,&nbsp;Jiehua Li ,&nbsp;Zongning Chen ,&nbsp;Yanjin Xu ,&nbsp;Tongmin Wang","doi":"10.1016/j.ultsonch.2024.107070","DOIUrl":"10.1016/j.ultsonch.2024.107070","url":null,"abstract":"<div><p>Metal additive manufacturing (AM) is a disruptive technology that provides unprecedented design freedom and manufacturing flexibility for the forming of complex components. Despite its unparalleled advantages over traditional manufacturing methods, the existence of fatal issues still seriously hinders its large-scale industrial application. Against this backdrop, U-FAAM is emerging as a focus, integrating ultrasonic energy into conventional metal AM processes to harness distinctive advantages. This work offers an up-to-date, specialized review of U-FAAM, articulating the integrated modes, mechanisms, pivotal research achievements, and future development trends in a systematic manner. By synthesizing existing research, it highlights future directions in further optimizing process parameters, expanding material applicability, etc., to advance the industrial application and development of U-FAAM technology.</p></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107070"},"PeriodicalIF":8.7,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1350417724003183/pdfft?md5=6b031774eb5959f95efef587d9ede212&pid=1-s2.0-S1350417724003183-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142241868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}