Ultrasonics Sonochemistry最新文献

{"title":"Deciphering the hydrodynamics of lipid-coated microbubble sonoluminescence for sonodynamic therapy","authors":"Priyankan Datta ,&nbsp;Sreejesh Moolayadukkam ,&nbsp;Rakesh Prasad Sahu ,&nbsp;Ranjan Ganguly ,&nbsp;Swarnendu Sen ,&nbsp;Ishwar K. Puri","doi":"10.1016/j.ultsonch.2024.107090","DOIUrl":"10.1016/j.ultsonch.2024.107090","url":null,"abstract":"<div><div>Sonodynamic therapy (SDT) is a minimally invasive targeted cancer therapy that uses focused low-intensity ultrasound (&lt;10 MPa, &lt;10 W/cm²) to activate sonosensitizer drugs. Once activated, these chemical compounds generate reactive oxygen species (ROS) to damage and kill cancer cells. A Phase I clinical trial has shown promising results for treating glioblastoma with SDT. We hypothesize that the efficacy of SDT can be improved by introducing lipid-coated microbubbles that produce a sonochemical effect that enhances ROS production. We investigate the hydrodynamics of a U.S. Food and Drug Administration (FDA)-approved microbubble, <em>Lumason</em>®, and a phospholipid-coated oxygen microbubble to predict the ultrasound parameters that induce sonoluminescence onset in biophysically relevant medium (e.g., water and blood) under clinical SDT conditions. The threshold pressures and frequencies for sonoluminescence with these therapeutic agents lie between 20 kHz – 1 MHz and 0.05 MPa – 1 MPa, respectively. The lipid-coated oxygen microbubble exhibits stronger sonoluminescence than the <em>Lumason</em>® microbubble, suggesting its use for improving SDT efficacy.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107090"},"PeriodicalIF":8.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted enzymatic extraction of jujube (Ziziphus jujuba Mill.) polysaccharides: Extraction efficiency, antioxidant activity, and structure features","authors":"Yiting Guo ,&nbsp;Shenao Nan ,&nbsp;Chengcheng Qiu ,&nbsp;Chenyu Song ,&nbsp;Bengang Wu ,&nbsp;Yanhua Tang ,&nbsp;Lifang Cheng ,&nbsp;Haile Ma","doi":"10.1016/j.ultsonch.2024.107088","DOIUrl":"10.1016/j.ultsonch.2024.107088","url":null,"abstract":"<div><div>This study investigated the effect of ultrasound-assisted enzymatic extraction (UAEE) on the extraction efficiency, antioxidant activity, and structural properties of jujube polysaccharide (JPS), with hot water extraction (HWE), ultrasound-assisted extraction (UAE), and enzymatic-assisted extraction (EAE) serving as controls. Optimal extraction conditions were determined through a multi-index weighted scoring method that comprehensively accounted for yield, duration, and antioxidant activity. Results demonstrated that the JPS yield obtained by UAEE at 22/33 kHz was 10.5 % to 16.3 % higher than those achieved by the other methods, significantly enhancing antioxidant activity. Monosaccharide composition analysis revealed that UAEE increased the content of key mono-sugars in JPS. Additionally, assessments of molecular weight distribution, zeta potential, and rheological properties showed that UAEE reduced the molecular weight and apparent viscosity of JPS, resulting in a looser structural configuration. These structural modifications were observed in scanning electron microscope (SEM) images, which revealed a filamentous branched morphology in JPS obtained through UAEE. Further observations using the atomic force microscope (AFM) indicated that the polysaccharide chains extracted by UAEE were shorter in length, lower in height, and free from aggregation.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107088"},"PeriodicalIF":8.7,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142359601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of extraction efficiency and functional properties of chickpea protein isolate using pulsed electric field combined with ultrasound treatment","authors":"Xin-Jue Lai ,&nbsp;Jian-Quan Chen ,&nbsp;Jing Nie ,&nbsp;Pei-Feng Guo ,&nbsp;Muhammad Faisal Manzoor ,&nbsp;Yan-Yan Huang ,&nbsp;Jian Li ,&nbsp;Song-Yi Lin ,&nbsp;Xin-An Zeng ,&nbsp;Rui Wang","doi":"10.1016/j.ultsonch.2024.107089","DOIUrl":"10.1016/j.ultsonch.2024.107089","url":null,"abstract":"<div><div>Chickpea protein isolate (CPI) is a promising dietary protein with the advantages of low allergenicity, easy digestion and balanced composition of essential amino acids. However, due to the thick skin of chickpeas, the extraction of CPI is challenging, resulting in lower efficiency of the alkaline extraction-isoelectric precipitation (AE-IEP) method. Therefore, the present study investigated the effect of pulsed electric field combined with ultrasound (PEF-US) treatment on the extraction efficiency of CPI and the functional properties was characterized. Parameter optimization was carried out using response surface methodology (RSM), with the following optimized conditions: pulse duration of 87 s, electric field intensity of 0.9 kV/cm, ultrasonic time of 15 min, and ultrasonic power of 325 W. Under the optimized conditions, the yield of CPI after combined (PEF-US) treatment was 13.52 ± 0.13 %, which was a 47.28 % improvement over the AE-IEP method. This yield was better than that obtained with either individual PEF or US treatment. Additionally, the functional properties (solubility, emulsification, and foaming) of CPI were significantly enhanced compared to AE-IEP. However, the stability of emulsification and foaming did not show significant differences among the four methods. The PEF-US method efficiently extracts CPI with excellent functional properties, enabling the production of proteins as desired functional additives in the food industry.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107089"},"PeriodicalIF":8.7,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142359600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preservation of minimally processed carrots using the combination of ultrasound and mild heat ascorbic acid","authors":"Jiayi Wang ,&nbsp;Ning Zhou ,&nbsp;Sen Ma ,&nbsp;Xiaofei Yang ,&nbsp;Jun Xing","doi":"10.1016/j.ultsonch.2024.107086","DOIUrl":"10.1016/j.ultsonch.2024.107086","url":null,"abstract":"<div><div>Ultrasound (US) in combination with chemical disinfectants is an efficient and cost-effective hurdle technology for disinfecting minimally processed produce (MPP). However, the demand for non-chemical disinfection methods is increasing. In addition, chemical methods have been ineffective in simultaneously improving the physiological properties and inactivating pathogens in MPP. In this study, a novel and safe method called mild heat ascorbic acid (MHAsA; 1 % AsA at 50 °C) was combined with US to process minimally processed carrots. Physiological properties and microbial inactivation efficacy were analyzed during the storage period (0–5 days). The findings indicated that US-MHAsA induced the highest levels of antioxidant enzymes (superoxide dismutase and catalase) activities and accelerated the glutathione-ascorbate cycle, resulting in lower reactive oxygen species (ROS) and malondialdehyde content compared to US and MHAsA. The efficacy of US-MHAsA in inactivating phenylalanine lyase, the initial enzyme in the lignin synthesis process, was lower than that of US. On the other hand, its ability to inactivate cinnamyl alcohol dehydrogenase, the final enzyme in the process, was better than that of both US and MHAsA. However, there were no significant differences in lignin content among the three groups. The inactivation efficacy against enzymes (polyphenol oxidase and peroxidase) involved in browning was consistent across the three treatments. Analysis of the disinfection efficacy against <em>Escherichia coli</em> O157:H7 and <em>Salmonella</em> Typhimurium revealed that US-MHAsA achieved the lowest cross-contamination incidence (10–12 %) during washing, which was significantly lower than the incidence achieved by US (75–82 %). During the period from day 0 to day 5, two pathogens on carrots in the control group increased from 6.25 to 6.64 log CFU/g, while the lowest counts were observed in the US-MHAsA group, decreasing from 4.44 to 3.74 log CFU/g. However, the counts in the US group increased from 5.22 to 6.32 log CFU/g, and the counts at day 5 were not significantly lower than the control. These findings indicate that US-MHAsA is a novel hurdle technology that effectively reduces the risk of pathogen contamination and enhances the ability of MPP to scavenge ROS.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107086"},"PeriodicalIF":8.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142329792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive review on the potential of ultrasound for blue food protein extraction, modification and impact on bioactive properties","authors":"Ghumika Pandita ,&nbsp;Savvy Sharma ,&nbsp;Irin Elsa Oommen ,&nbsp;Nishchhal Madaan ,&nbsp;Yuvraj Bhosale ,&nbsp;Vivien Nagy ,&nbsp;Ayaz Mukarram Shaikh ,&nbsp;Béla Kovács","doi":"10.1016/j.ultsonch.2024.107087","DOIUrl":"10.1016/j.ultsonch.2024.107087","url":null,"abstract":"<div><div>Food security for the increasing global population is a significant challenge of the current times particularly highlighting the protein deficiencies. Plant-based proteins could be considered as alternate source of the protein. The digestibility and PDCASS value of these proteins are still a concern. Blue proteins, the new approach of utilizing the proteins from aquatic sources could be a possible solution as it contains all the essential amino acids. However, the conjugation of these proteins with fats and glycogen interferes with their techno-functional properties and consequently their applicability. The application of power ultrasound for extraction and modification of these proteins from aquatic sources to break open the cellular structure, increase extractability, alter the protein structure and consequently provide proteins with higher bioavailability and bioactive properties could be a potential approach for their effective utilization into food systems. The current review focuses on the application of power ultrasound when applied as extraction treatment, alters the sulphite and peptide bond and modifies protein to elevated digestibility. The degree of alteration is influenced by intensity, frequency, and exposure time. The extracted proteins will serve as a source of essential amino acids. Furthermore, modification will lead to the development of bioactive peptides with different functional applications. Numerous studies reveal that blue proteins have beneficial impacts on amino acid availability, and subsequently food security with higher PDCAAS values. In many cases, converted peptides give anti-hypertensive, anti-diabetic, and anti-oxidant activity. Therefore, researchers are concentrating on ultrasound-based extraction, modification, and application in food and pharmaceutical systems.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107087"},"PeriodicalIF":8.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of ultrafine Himalayan walnut oil Pickering emulsions by ultrasonic emulsification: Techno-functional properties of emulsions and microcapsules","authors":"Gazalla Akhtar,&nbsp;F.A. Masoodi,&nbsp;Sabeera Muzaffar","doi":"10.1016/j.ultsonch.2024.107081","DOIUrl":"10.1016/j.ultsonch.2024.107081","url":null,"abstract":"<div><div>In present scenario, much of the attention has been put on the production and utilization of Pickering emulsions deciphering enhanced stability and applicability over wide environmental conditions. In this context the present study was carried out to elaborate effect of different wall materials and pH systems on the physicochemical, structural and morphological properties of Himalayan walnut oil Pickering emulsions by ultrasonic emulsification. In this study, concentrated Pickering emulsion of Himalayan walnut oil (HWO) was prepared utilizing soy protein isolate (SPI), maltodextrin (MD) stabilized by pectin at varying concentrations and pH systems (4.0, 7.0). With increase in pectin and SPI concentration and lowering MD, stable emulsions were obtained as deciphered by an Emulsion stability index (ESI) of 100 for 7 days at ambient storage. HWO Pickering emulsions were analysed for particle size measurements (2.13–13.64 µm) and depicted negative zeta potential values (−3.70 to −18.58). Lyophilized HWO microcapsules depicted moderate encapsulation efficiency (44.69–57.63 %) whereas the hygroscopicity values of the microcapsule ranged from (0.21–12.10 %). Thermogravimetric analysis (TGA) of the samples depicted the temperature of maximum degradation rate up to 550 °C whereas XRD spectra depicted amorphous nature of oil microcapsules. FTIR spectra revealed a close association between the SPI-MD-Pectin matrix. SEM analysis revealed stable oil globules entrapped in protein-polysaccharide matrix with no visible cracks and fissures.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107081"},"PeriodicalIF":8.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The molecular impact of sonoporation: A transcriptomic analysis of gene regulation profile","authors":"Xinxing Duan ,&nbsp;Jennifer M.F. Wan ,&nbsp;Alfred C.H. Yu","doi":"10.1016/j.ultsonch.2024.107077","DOIUrl":"10.1016/j.ultsonch.2024.107077","url":null,"abstract":"<div><div>Sonoporation has long been known to disrupt intracellular signaling, yet the involved molecules and pathways have not been identified with clarity. In this study, we employed whole transcriptome shotgun sequencing (RNA-seq) to profile sonoporation-induced gene responses after membrane resealing has taken place. Sonoporation was achieved by microbubble-mediated ultrasound (MB-US) exposure in the form of 1 MHz ultrasound pulsing (0.50 MPa peak negative pressure, 10 % duty cycle, 30 s exposure period) in the presence of microbubbles (1:1 cell-to-bubble ratio). Using propidium iodide (PI) and calcein respectively as cell viability and cytoplasmic uptake labels, post-exposure flow cytometry was performed to identify three viable cell populations: 1) unsonoporated cells, 2) sonoporated cells with low uptake, and 3) sonoporated cells with high uptake. Fluorescence-activated cell sorting was then conducted to separate the different groups followed by RNA-seq analysis of the gene expressions in each group of cells. We found that sonoporated cells with low or high calcein uptake showed high similarity in the gene responses, including the activation of multiple heat shock protein (HSP) genes and immediate early response genes mediating apoptosis and transcriptional regulation. In contrast, unsonoporated cells exhibited a more extensive gene expression alteration that included the activation of more HSP genes and the upregulation of diverse apoptotic mediators. Four oxidative stress-related and three immune-related genes were also differentially expressed in unsonoporated cells. Our results provided new information for understanding the intracellular mobilization in response to sonoporation at the molecular level, including the identification of new molecules in the sonoporation-induced apoptosis regulatory network. Our data also shed light on the innovative therapeutic strategy which could potentially leverage the responses of viable unsonoporated cells as a synergistic effector in the microenvironment to favor tumor treatment.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107077"},"PeriodicalIF":8.7,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of ultrasonic and heat treatments on the physicochemical properties and rennet-induced coagulation characteristics of milk from various species","authors":"Wenjuan Hou ,&nbsp;Xuli Ma ,&nbsp;Zhongna Yu ,&nbsp;Latiful Bari ,&nbsp;Hongning Jiang ,&nbsp;Qijing Du ,&nbsp;Rongbo Fan ,&nbsp;Jun Wang ,&nbsp;Yongxin Yang ,&nbsp;Rongwei Han","doi":"10.1016/j.ultsonch.2024.107084","DOIUrl":"10.1016/j.ultsonch.2024.107084","url":null,"abstract":"<div><div>This study investigates the effects of heat and ultrasonic treatments on the physicochemical parameters and rennet-induced coagulation properties of milk from a variety of species, including cow, goat, buffalo, and donkey. Milk samples were subjected to heat treatments at different temperatures (65 °C, 80 °C, 90 °C, 100 °C) and ultrasonic treatment at varying power levels (200 W, 400 W, 600 W, 800 W, 1000 W). The results revealed that changes in turbidity, particle size, zeta potential, secondary structure, and surface hydrophobicity were altered by both ultrasonic and heat treatments, as well as the kind of milk. Ultrasonic treatment of cow milk decreased α-helix content while increasing β-turn content. Under similar ultrasonic treatment, goat milk showed a considerable increase in β-sheet content, whereas β-turn and random coil contents decreased compared to control samples. Notably, the water-holding capacity of gels formed from all four types of milk increased significantly with the intensity of ultrasonic and heat treatments. The hardness of buffalo milk gels increased significantly after ultrasonic and thermal treatments, ranging from 63 °C for 30 min to 90 °C for 15 min, but the hardness of cow and goat milk gels increased in varying degrees compared to their control samples. Furthermore, gels from cow and goat milk had higher storage modulus (G’) and loss modulus (G’’) than those from buffalo and donkey milk, and changes in G’ and G’’ from the examined milk were altered by ultrasonic and heat treatments. These findings offer important insights into refining milk processing procedures to improve dairy product quality and usefulness.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107084"},"PeriodicalIF":8.7,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142363887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural characterization, antioxidant activity, and fermentation characteristics of Flammulina velutipes residue polysaccharide degraded by ultrasonic assisted H2O2-Vc technique","authors":"Yunxiang Que,&nbsp;Yao Zhang,&nbsp;Fengxiang Liang,&nbsp;Liping Wang,&nbsp;Yiting Yang,&nbsp;Jingbo Zhang,&nbsp;Wanting Wang,&nbsp;Ying Sun,&nbsp;Changjiao Zhong,&nbsp;Haipeng Zhang,&nbsp;Chengguang He,&nbsp;Lili Guan,&nbsp;Hongxia Ma","doi":"10.1016/j.ultsonch.2024.107085","DOIUrl":"10.1016/j.ultsonch.2024.107085","url":null,"abstract":"<div><div>Adhere to the concept of low-carbon environmental protection and turning waste into treasure, polysaccharides from <em>Flammulina velutipes</em> residue polysaccharide (FVRP) has been developed and possesses diverse bioactivities, comprising antioxidant, hypoglycemic, and relieving heavy metal damage, which still has the disadvantages of high molecular weight and low bioavailability. The current work is the first to prepare a degraded polysaccharide (FVRPV) from FVRP by ultrasonic assisted H₂O₂-Vc technique in order to reduce its molecular weight, thereby improving its activity and bioavailability. Our results found that the molecular weight and average particle size were declined, but the monosaccharide composition and characteristic functional group types of FVRPV had no impact. The structural changes of polysaccharides analyzed by XRD, Congo Red test, I₂-KI, SEM, and methylation analysis indicated that the surface morphology and glycosidic bond composition of FVRPV possessed longer side chains and a greater number of branches with an amorphous crystal structure devoid of a triple helix configuration, and had experienced notable alterations after ultrasonic assisted H₂O₂-Vc treatment. Meanwhile, the <em>in vitro</em> antioxidant capacity of FVRPV had significantly increased compared to FVRP, implying ultrasonic assisted H₂O₂-Vc technique maybe a effective method to enhance the bioactivity of polysaccharides. In addition, the content of polysaccharide, reducing sugar, and uronic acid in FVRPV was significantly decreased, but antioxidant capacity of fermentation broth was stronger by <em>in vitro</em> human fecal fermentation. The 16S rDNA sequencing data displayed that FVRPV can enrich probiotics and reduce the abundance of pathogenic bacteria through different metabolic pathways mediated by gut microbiota, thereby exerting its potential probiotic effects. The interesting work provides a novel degraded polysaccharide by ultrasonic assisted H₂O₂-Vc technique, laying a foundation for developing FVRPV as a new antioxidant and prebiotic.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107085"},"PeriodicalIF":8.7,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted extraction of polyphenols from Phyllanthi Fructus: Comprehensive insights from extraction optimization and antioxidant activity","authors":"Haixia Che ,&nbsp;Rui Zhang ,&nbsp;Xianyao Wang ,&nbsp;Hong Yu ,&nbsp;Xuepeng Shi ,&nbsp;Jie Yi ,&nbsp;Jiawen Li ,&nbsp;Qi Qi ,&nbsp;Ruizhi Dong ,&nbsp;Qian Li","doi":"10.1016/j.ultsonch.2024.107083","DOIUrl":"10.1016/j.ultsonch.2024.107083","url":null,"abstract":"<div><div>Phyllanthi Fructus (PF) is a valuable botanical resource with a long history of traditional use, known for potent antioxidant and anti-inflammatory effects attributed to its rich contents of bioactive compounds, particularly polyphenols. However, current extraction techniques limit the utilization of polyphenols from PF. This study aimed to achieve the maximum polyphenol yield and improve the antioxidant activity of PF extracts to promise PF’s prospects for modern healthcare.</div><div>Firstly, ultrasonic-assisted extraction (UAE) was employed to extract the polyphenols in PF and a combination of Plackett-Burman designs (PBD) and response surface methodology (RSM) was applied to optimize UAE’s conditions. Next, cellular superoxide dismutase (SOD) and malondialdehyde (MDA) were used to assess the antioxidant activity of extracted polyphenols. Ultra-Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-TOF MS) was utilized to characterize polyphenol components in the PF extracts. Finally, network pharmacology and molecular docking analysis were performed to screen the potential target proteins of polyphenols from PF.</div><div>As a result, the optimized polyphenol yield was 213.49 mg/g, and the antioxidant activities, measured by ability of DPPH scavenging, ABTS+ scavenging, and FRAP were 76.95 %, 2.24 mmol/g, 2.34 mmol/g, respectively. PF extracts also showed good antioxidant capabilities at cellular level. 26 polyphenol components were identified in the PF extracts. Among these, ellagic acid, myricetin, and eriodictyol may exert antioxidant effects by interacting with AKT serine/threonine kinase 1 (AKT1).</div><div>In conclusion, our study provides valuable insights into the optimizing PF extraction and underscores its potential applications in enhancing natural polyphenols extraction using UAE with a combination of PBD and RSM. These findings offer a promising avenue for the development and utilization of PF, and could serve as a reference for similar extraction processes in the future.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107083"},"PeriodicalIF":8.7,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}