三黄茯苓黄酮类化合物超声辅助优化提取及生物活性分析

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2025-04-06 DOI:10.1016/j.ultsonch.2025.107326

Yanhua Wang , Chen Zhang , Yilin Zhao , Fuhua Wu , Yaoli Yue , Yingjun Zhang , Dandan Li

{"title":"三黄茯苓黄酮类化合物超声辅助优化提取及生物活性分析","authors":"Yanhua Wang , Chen Zhang , Yilin Zhao , Fuhua Wu , Yaoli Yue , Yingjun Zhang , Dandan Li","doi":"10.1016/j.ultsonch.2025.107326","DOIUrl":null,"url":null,"abstract":"<div><div>The fungus Sanghuangporus sanghuang possesses notable medicinal and edible characteristics, displaying a diverse array of biological functionalities. This research endeavor seeks to investigate the procedure of extracting flavonoids from S. sanghuang, and the qualitative and quantitative analysis of flavonoids extraction from S. sanghuang using ultra-performance liquid chromatography (UPLC), and assess its antioxidant capacity and potential antiproliferative properties. The ultrasonic-assisted extraction resulted in a 2.34-fold increase compared to the hot water extraction method. Response surface methodology (RSM) was employed to enhance the extraction process of flavonoids from S. sanghuang. The results indicated that the optimal extraction rate of S. sanghuang flavonoids were achieved at 16.16 ± 0.12 %. This was attained at an ultrasound temperature of 50°C using 80 % ethanol concentration and an ultrasound extraction time of 60 min. The S. sanghuang extract was analyzed using UPLC, resulting in the identification of twenty-six distinct compounds. The flavonoids derived from S. sanghuang have demonstrated the ability to effectively scavenge DPPH, superoxide anions (O2–·), and hydroxyl free radicals (·OH), in addition to exhibiting ferric reducing power. Furthermore, it exhibited inhibitory effects on α-glucosidase. The Pearson correlation analysis revealed a statistically significant positive correlation between the antioxidant capacities, encompassing DPPH, O2–·, ·OH, ferric reducing power, and the inhibited α-glucosidase capability. It has been determined that the activity of α-glucosidase can be inhibited by S. sanghuang flavonoids, and this inhibition can be predicted using a model developed with the MATLAB program. In the current investigation, the study successfully demonstrated the inhibitory effects of S. sanghuang flavonoids on cell proliferation and migration in glioma cells. This was achieved through the analysis of CCK-8 assay and wound healing assay, with statistical significance observed (p < 0.05).</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"117 ","pages":"Article 107326"},"PeriodicalIF":8.7000,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ultrasound-assisted optimization extraction and biological activities analysis of flavonoids from Sanghuangporus sanghuang\",\"authors\":\"Yanhua Wang , Chen Zhang , Yilin Zhao , Fuhua Wu , Yaoli Yue , Yingjun Zhang , Dandan Li\",\"doi\":\"10.1016/j.ultsonch.2025.107326\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The fungus Sanghuangporus sanghuang possesses notable medicinal and edible characteristics, displaying a diverse array of biological functionalities. This research endeavor seeks to investigate the procedure of extracting flavonoids from S. sanghuang, and the qualitative and quantitative analysis of flavonoids extraction from S. sanghuang using ultra-performance liquid chromatography (UPLC), and assess its antioxidant capacity and potential antiproliferative properties. The ultrasonic-assisted extraction resulted in a 2.34-fold increase compared to the hot water extraction method. Response surface methodology (RSM) was employed to enhance the extraction process of flavonoids from S. sanghuang. The results indicated that the optimal extraction rate of S. sanghuang flavonoids were achieved at 16.16 ± 0.12 %. This was attained at an ultrasound temperature of 50°C using 80 % ethanol concentration and an ultrasound extraction time of 60 min. The S. sanghuang extract was analyzed using UPLC, resulting in the identification of twenty-six distinct compounds. The flavonoids derived from S. sanghuang have demonstrated the ability to effectively scavenge DPPH, superoxide anions (O2–·), and hydroxyl free radicals (·OH), in addition to exhibiting ferric reducing power. Furthermore, it exhibited inhibitory effects on α-glucosidase. The Pearson correlation analysis revealed a statistically significant positive correlation between the antioxidant capacities, encompassing DPPH, O2–·, ·OH, ferric reducing power, and the inhibited α-glucosidase capability. It has been determined that the activity of α-glucosidase can be inhibited by S. sanghuang flavonoids, and this inhibition can be predicted using a model developed with the MATLAB program. In the current investigation, the study successfully demonstrated the inhibitory effects of S. sanghuang flavonoids on cell proliferation and migration in glioma cells. This was achieved through the analysis of CCK-8 assay and wound healing assay, with statistical significance observed (p < 0.05).</div></div>\",\"PeriodicalId\":442,\"journal\":{\"name\":\"Ultrasonics Sonochemistry\",\"volume\":\"117 \",\"pages\":\"Article 107326\"},\"PeriodicalIF\":8.7000,\"publicationDate\":\"2025-04-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ultrasonics Sonochemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1350417725001051\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ACOUSTICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ultrasonics Sonochemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1350417725001051","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ACOUSTICS","Score":null,"Total":0}

引用次数: 0

摘要

桑黄真菌具有显著的药用和食用特性，显示出多种多样的生物功能。本研究试图探讨从桑黄菌中提取黄酮类化合物的程序，并利用超高效液相色谱法（UPLC）对桑黄菌中提取的黄酮类化合物进行定性和定量分析，评估其抗氧化能力和潜在的抗增殖特性。与热水提取法相比，超声波辅助提取法的提取率提高了 2.34 倍。采用响应面法（RSM）改进了从桑黄中提取黄酮类化合物的过程。结果表明，黄连黄酮的最佳提取率为 16.16 ± 0.12 %。最佳萃取率是在超声温度为 50°C、乙醇浓度为 80%、超声萃取时间为 60 分钟的条件下实现的。使用 UPLC 对山苍子提取物进行分析，鉴定出 26 种不同的化合物。结果表明，桑黄提取物中的黄酮类化合物能有效清除 DPPH、超氧阴离子（O2--）和羟基自由基（-OH），此外还具有铁还原力。此外，它还对α-葡萄糖苷酶有抑制作用。皮尔逊相关分析表明，抗氧化能力（包括 DPPH、O2--、-OH、铁还原力）与抑制α-葡萄糖苷酶的能力之间存在统计学意义上的显著正相关。研究发现，桑黄黄酮类化合物可抑制α-葡萄糖苷酶的活性，这种抑制作用可通过使用 MATLAB 程序开发的模型进行预测。在本次研究中，研究人员成功地证明了山黄素对胶质瘤细胞增殖和迁移的抑制作用。这一点是通过 CCK-8 检测和伤口愈合检测分析实现的，并观察到了统计学意义（p < 0.05）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Ultrasound-assisted optimization extraction and biological activities analysis of flavonoids from Sanghuangporus sanghuang

The fungus Sanghuangporus sanghuang possesses notable medicinal and edible characteristics, displaying a diverse array of biological functionalities. This research endeavor seeks to investigate the procedure of extracting flavonoids from S. sanghuang, and the qualitative and quantitative analysis of flavonoids extraction from S. sanghuang using ultra-performance liquid chromatography (UPLC), and assess its antioxidant capacity and potential antiproliferative properties. The ultrasonic-assisted extraction resulted in a 2.34-fold increase compared to the hot water extraction method. Response surface methodology (RSM) was employed to enhance the extraction process of flavonoids from S. sanghuang. The results indicated that the optimal extraction rate of S. sanghuang flavonoids were achieved at 16.16 ± 0.12 %. This was attained at an ultrasound temperature of 50°C using 80 % ethanol concentration and an ultrasound extraction time of 60 min. The S. sanghuang extract was analyzed using UPLC, resulting in the identification of twenty-six distinct compounds. The flavonoids derived from S. sanghuang have demonstrated the ability to effectively scavenge DPPH, superoxide anions (O₂^–·), and hydroxyl free radicals (^·OH), in addition to exhibiting ferric reducing power. Furthermore, it exhibited inhibitory effects on α-glucosidase. The Pearson correlation analysis revealed a statistically significant positive correlation between the antioxidant capacities, encompassing DPPH, O₂^–·, ^·OH, ferric reducing power, and the inhibited α-glucosidase capability. It has been determined that the activity of α-glucosidase can be inhibited by S. sanghuang flavonoids, and this inhibition can be predicted using a model developed with the MATLAB program. In the current investigation, the study successfully demonstrated the inhibitory effects of S. sanghuang flavonoids on cell proliferation and migration in glioma cells. This was achieved through the analysis of CCK-8 assay and wound healing assay, with statistical significance observed (p < 0.05).

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.