Enhancing ultrasound applications through shell-less nanobubbles: A study on acoustic and optical properties

Histotripsy employs acoustic inertial cavitation to mechanically destroy tissue, producing acellular debris. While introducing bubbles can lower the cavitation threshold and enhance treatment efficiency, micrometer-scale bubbles struggle to penetrate tissues effectively. Shell-less nanobubbles, with their high internal pressure, stability, negatively charged surfaces, and unique lifetimes ranging from weeks to months, offer a promising alternative. However, their interactions with ultrasound remain unexplored. This study used a claw-type pump nanobubble generator to produce nanobubbles and employed acoustic and optical methods to observe their behavior under high-intensity ultrasound exposure. The results demonstrated that the device generated nanobubble solutions with an average particle size of 107 nm, a concentration of 1.94 × 10⁹ particles/mL, a lifetime exceeding one week, and a zeta potential of −21.2 mV. Acoustic and optical observations further revealed that nanobubble solutions reduced the inertial cavitation threshold of the liquid from 26.5 MPa to 10.3 MPa. These findings suggest a potential strategy to enhance the efficiency of ultrasound histotripsy treatments.