Microchemical Journal最新文献

{"title":"Label-free electrochemical aptasensor based on cellulose nanocrystal-modified paper-based device for Salmonella Typhimurium detection in food samples","authors":"Sakda Jampasa ,&nbsp;Natthawut Sangthong ,&nbsp;Tugba Ozer ,&nbsp;Wattana Panphut ,&nbsp;Sarida Naorungroj ,&nbsp;Nattaya Ngamrojanavanich ,&nbsp;Takashi Kaneta ,&nbsp;Orawon Chailapakul ,&nbsp;Wanida Wonsawat","doi":"10.1016/j.microc.2025.113144","DOIUrl":"10.1016/j.microc.2025.113144","url":null,"abstract":"<div><div><em>Salmonella Typhimurium</em> (<em>S. Typhimurium</em>) is a pathogenic bacterium associated with food contamination and severe illnesses. Given its low infectious dose, monitoring this bacterium is critical. Conventional detection methods often involve labor-intensive and time-consuming enzyme-linked immunoassays, further highlighting the need for more efficient alternatives. In this study, we developed a paper-based electrochemical aptasensor utilizing a label-free format, enhanced with carboxyl (COOH)-functionalized cellulose nanocrystals, as an innovative platform for detecting <em>S. Typhimurium</em>. The device was custom-designed and fabricated using a wax printing technique on Whatman filter paper, yielding a disposable and desirable paper-based analytical device (PAD). COOH-cellulose nanocrystals were applied to the PAD surface to directly immobilize an aptamer probe specific to <em>Salmonella</em>, employing effective EDC/NHS standard chemistry without requiring additional modifications. The subsequent formation of a half-sandwich immunocomplex (aptamer/<em>Salmonella</em>) induces a highly insulated layer on the PAD surface, suppressing the redox mediator signal response of [Fe(CN)₆]^3-/4- in a <em>Salmonella</em> concentration-dependent manner, with detectable ranges from 10 to 10⁹ CFU/mL and an ultra-low detection limit of 3.50 CFU/mL, as evaluated by differential pulse voltammetry. This aptasensor successfully detects the presence of <em>Salmonella</em> in food samples, demonstrating remarkable sensitivity, selectivity, rapid response times (30 min), and portability.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113144"},"PeriodicalIF":4.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of solid-phase microextraction fibers based on hybrid MIL-68(Fe)@MIL-101(Fe) for the analysis of polycyclic aromatic hydrocarbons in surface waters","authors":"Sol Giovannoni ,&nbsp;Gabriela A. Ortega-Moreno ,&nbsp;Griselda Narda ,&nbsp;Mario Reta ,&nbsp;Carlina Lancioni","doi":"10.1016/j.microc.2025.113143","DOIUrl":"10.1016/j.microc.2025.113143","url":null,"abstract":"<div><div>The development of solid-phase microextraction fibers (SPME) using a MOF-based hybrid material as sorbent is presented. Even though different metal–organic frameworks (MOFs) have been reported as extractant phases, it is the first time that a hybrid material consisting of two iron-based MOFs is used with this purpose. In this work, the synthesis and characterization of the hybrid MIL-68(Fe)@MIL-101(Fe), as well as, the construction and characterization of the SPME fibers, is detailed. The assessment of its analytical performance, was studied by analyzing five polycyclic aromatic hydrocarbons (PAHs) in aqueous solution by means of gas chromatography coupled to flame ionization and mass spectrometry detectors. The developed analytical method exhibited detection limits between 3 to 10 μg·L⁻¹, good linearity (R² &gt; 0.96) and remarkable intra-day (average RSD%=10) and inter-day (average RSD% = 14) precision. Moreover, enrichment factors ranged from 187 to 273-fold while accuracies (expressed as percentage recoveries) ranged from 64 to 111 %, and 84 to 123 % for low and high concentration levels, respectively. The developed method was successfully applied to the determination and quantification of PAHs in surface water samples, collected from two streams located in La Plata (Buenos Aires Province, Argentina).</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113143"},"PeriodicalIF":4.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative ferrofluid-based vortex-assisted dispersive liquid-liquid microextraction of cadmium and nickel using novel and natural hydrophobic deep eutectic solvent and 2-methylimidazole-coated magnetic nanoparticles","authors":"Mustafa Soylak ,&nbsp;Qamar Salamat ,&nbsp;Sabrina Sajjad","doi":"10.1016/j.microc.2025.113050","DOIUrl":"10.1016/j.microc.2025.113050","url":null,"abstract":"<div><div>This study presents a novel, natural, and cost-effective ferrofluid based on a deep eutectic solvent (DES), utilizing a new hydrophobic DES composed of menthol and octylamine as the carrier medium and magnetic nanoparticles with novel surface modification using 2-methylimidazole (2-MIM). The produced ferrofluid was characterized using FTIR, XRD, SEM, and SEM-EDX techniques. This ferrofluid served as an effective extraction solvent for the fast preconcentration and extraction of Cd(II) and Ni(II) from diverse actual samples utilizing vortex-assisted dispersive liquid–liquid microextraction (VA-DLLME) followed by flame atomic absorption spectrometry (FAAS). The optimization of key extraction parameters, such as pH, ferrofluid quantity, adsorption duration, and eluent characteristics, was conducted utilizing the one-variable-at-a-time (OVAT) approach. Under ideal conditions, the method exhibited a linear calibration range of 0.05–5.0 μg mL⁻¹, with determination coefficients (R²) of 0.9893 for Cd(II) and 0.9936 for Ni(II). The limits of detection (LOD) were 0.015 μg mL⁻¹, with relative standard deviations (RSDs) of 2.9 % (Cd(II)) and 2.6 % (Ni(II)) for intra-day precision, and 3.1 % (Cd(II)) and 4.9 % (Ni(II)) for inter-day precision. The approach attained preconcentration factors of 5.0, enrichment factors of 4.8 and 4.7, and extraction recoveries of 96 % and 94 % for Cd(II) and Ni(II), respectively. The methodology was corroborated utilizing certified reference materials (BCR-505, NCS ZC73032, and NIST 1573a). The DES-FF/VA-DLLME technique exhibited effective and swift extraction of Cd(II) and Ni(II) from aqueous and food matrices, providing a speedy, eco-friendly, and sustainable approach to analytical chemistry.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113050"},"PeriodicalIF":4.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sandwich-type electrochemical aptamer-based sensor for rapid nanomolar detection of anesthetic drug procaine in biofluids","authors":"Yu Xie ,&nbsp;Jiawen Yin ,&nbsp;Fei Deng ,&nbsp;Lingfeng Xu ,&nbsp;Kalle Salminen ,&nbsp;Limin Liu","doi":"10.1016/j.microc.2025.113147","DOIUrl":"10.1016/j.microc.2025.113147","url":null,"abstract":"<div><div>It is important to develop clinical point-of-care testing (POCT) methods to avoid side effects and even fatalities due to anesthetic drug overdose. Due to their inherent advantages (e.g. ease of modification, thermal stability, and low cost), electrochemical aptamer-based (EAB) sensors are promising alternatives to conventional analytical methods. However, most of the current methods require some complex modification steps for the preparation of electrodes or signal probes as well as the indirect measurement of signals, and the application of these methods as POCT devices remains a challenge. In this work, a sandwich-type EAB sensor was developed for the direct determination of procaine in biofluids. The original aptamer was split into two segments, with one fragment modified with thiol and the other modified with a redox label. The detection process was rapid as conformational changes occur almost instantaneously when the aptamer binds to the target. Moreover, the binding properties of the aptamer towards the target were characterized by circular dichroism spectroscopy, molecular docking simulations and apparent electron transfer rates test. The simple, cost-effective and reagentless sandwich-type EAB sensor achieved nanomolar detection of procaine with a limit of detection (LOD) of 1.9 nM, 5.4 nM and 3.3 nM in buffer, 5 % urine and 2.5 % serum, respectively. This work is expected to play a role in the field of clinical drug analysis.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113147"},"PeriodicalIF":4.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data fusion of Fourier transform infrared spectroscopy and high-performance liquid chromatography for the origin identification of different medicinal rhizomes of genus Atractylodes","authors":"Hongfei Wu ,&nbsp;Mingjun Wang ,&nbsp;Zhiming Zeng ,&nbsp;Changyun Dai ,&nbsp;Feilong Ren ,&nbsp;Hongbo Yin ,&nbsp;Lu Chen","doi":"10.1016/j.microc.2025.113110","DOIUrl":"10.1016/j.microc.2025.113110","url":null,"abstract":"<div><div>The rhizomes of the genus <em>Atractylodes</em>, such as <em>A. lancea</em>, <em>A. chinensis</em>, <em>A. japonica</em>, <em>A. coreana</em>, and <em>A. macrocephala</em>, have been extensively utilized as prominent traditional herbal medicines across China, Japan, South Korea and other Asian countries. Due to the close genetic relationships and morphological similarities, confusion and misuse frequently arise. Although various methods exist to identify <em>Atractylodes</em> species based on their chemical profiles, they often focus on a limited subset, overlooking species like <em>A. coreana</em> which are frequently observed in the traditional herbal medicine market in northern China and limiting comprehensive identification. In this study, data fusion of spectral and chromatographic data was used for the first time to identify five different medicinal rhizomes derived from genus <em>Atractylodes</em>. It also serves as a preliminary exploration of the integration of Fourier Transform Infrared Spectroscopy (FTIR), High-Performance Liquid Chromatography (HPLC) fingerprinting, and chemical pattern recognition within the domain of origin identification of traditional herbal medicines. Our study demonstrated that the mid-level data fusion of FTIR and HPLC data using partial least squares-discriminant analysis (PLS-DA) and t-distributed stochastic neighbor embedding (t-SNE) constituted an effective approach for identification. While PLS-DA excels in supervised classification, t-SNE complements it by offering intuitive visualization of high-dimensional data, revealing clustering patterns among the species. The 81 batches of dried rhizomes from five species of <em>Atractylodes</em> were divided into training and prediction sets at a 2:1 ratio, employing the K-S algorithm, achieving a prediction accuracy of 100%. The integration of t-SNE further confirmed the separation achieved by PLS-DA, enhancing the interpretability of the classification results and highlighting the potential of data fusion combined with advanced visualization techniques in distinguishing closely related herbal species. Additionally, the results showed that the chemical differences of <em>Atractylodes</em> among various varieties were mainly reflected in polysaccharides, alkynes, and ketones, the chemical composition of <em>A. macrocephala</em> was very different from that of other species, while <em>A. japonica</em> was close to that of <em>A. coreana</em>. This may indicate the genetic distance among them. It can successfully distinguish the five often-confused medicinal rhizomes of <em>Atractylodes</em>, achieving a prediction accuracy of 100%. This study presents a feasible approach for identifying five closely related medicinal rhizomes of <em>Atractylodes</em> using data fusion, demonstrating its potential in addressing challenges associated with distinguishing morphologically similar herbal medicines.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113110"},"PeriodicalIF":4.9,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intelligent microscopic imaging system based on microwell array chip for high-throughput analysis of single-cell heterogeneity","authors":"Lingzhi Ye ,&nbsp;Rui Deng ,&nbsp;Aiping Zhi ,&nbsp;Duo Sun ,&nbsp;Wei Ye ,&nbsp;Shi Hu ,&nbsp;Tingting Zhan ,&nbsp;Rui Hao ,&nbsp;Xi Chen ,&nbsp;Bin Sun ,&nbsp;Chunyan Liu ,&nbsp;Lin Dang ,&nbsp;Meng Li ,&nbsp;Zebin Chen ,&nbsp;Lin Zeng ,&nbsp;Jienan Shen ,&nbsp;Xiangmeng Qu ,&nbsp;Hui Yang","doi":"10.1016/j.microc.2025.113136","DOIUrl":"10.1016/j.microc.2025.113136","url":null,"abstract":"<div><div>With the increasing importance of single-cell analysis in many fields, such as biology and medicine, developing efficient and accurate high-throughput single-cell analysis technology has become a research hotspot. Herein, an intelligent microscopic imaging system based on microarray chips is reported to achieve high-throughput analysis of single cells. The system integrates a special microwell array chip, which can accurately locate and fix many single cells, providing a stable basis for the subsequent single-cell heterogeneity analysis. At the same time, it is equipped with an intelligent microscopic imaging module, which can obtain single-cell image data quickly and efficiently through an automated image acquisition program. The system, combined with advanced image analysis algorithms, can accurately extract key features such as the morphology and fluorescence signals of single cells from massive images and then realize in-depth analysis of multiple biological characteristics of single cells. As a proof of concept, six different types of cancer cells were selected A549, H1299, CT2A, SAOS-2, SH-SY5Y, U87, respectively. By processing 3900 microwell arrays on the chip, 2000 single cells were located and fixed simultaneously. The experimental results showed that the intelligent microscopic imaging system had shown good accuracy (90 %), repeatability, and high efficiency in high-throughput single-cell analysis, which is expected to provide strong technical support for many fields such as basic research in life science, disease diagnosis, and drug development, and significantly promote the research progress at the single-cell level.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113136"},"PeriodicalIF":4.9,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ag/MnO/GCE electrode for accurate and rapid ammonium ion detection","authors":"Jianmin Yang,&nbsp;Hongxiu Lu,&nbsp;Kai Tang,&nbsp;Changxiang Yang,&nbsp;Gang Wang,&nbsp;Aidong Tang","doi":"10.1016/j.microc.2025.113102","DOIUrl":"10.1016/j.microc.2025.113102","url":null,"abstract":"<div><div>Ammonia nitrogen mainly comes from industrial wastewater and aquaculture water bodies, and the construction of a rapid and accurate detection method is of great significance for assessing and monitoring the pollution status of water bodies and the risk of eutrophication. In this study, an Ag/MnO/ Glassy carbon electrode (GCE) electrochemical sensor was constructed by the pulsed deposition method, which can rapidly, accurately and sensitively detect ammonia nitrogen at low concentration under neutral environment. MnO, as the main catalyst, can generate H₂O₂ to oxidise ammonia nitrogen, thus achieving sensitive detection of ammonia nitrogen. Meanwhile, Ag can significantly improve the sensitivity and stability of the sensor. The Ag/MnO/GCE sensor showed a linear range of 0.05–150 μM for the detection of ammonia nitrogen, with a detection limit of 0.015 μM, a sensitivity of 195.50 μA mM⁻¹ cm⁻², and ideal recoveries for the detection of actual samples (93.60 %–101.26 %). The sensor exhibited fast response, good stability and selectivity, and little interference from common ions in water, which offers a workable strategy for the accurate and rapid detection of ammonia nitrogen in water.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113102"},"PeriodicalIF":4.9,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RAA-CRISPR/Cas13a-assisted lateral flow strip for Feline herpesvirus Type I point-of-care testing","authors":"Qin Wu ,&nbsp;Gan Lin ,&nbsp;Junfeng Li ,&nbsp;Binjie Zhu ,&nbsp;Chenxi Lei ,&nbsp;Zheqi Zhou ,&nbsp;Yuhe Fu ,&nbsp;Lei Lei ,&nbsp;Jiali Xu ,&nbsp;Jing Xia ,&nbsp;Lingli Jiang ,&nbsp;Houhui Song ,&nbsp;Changyong Cheng","doi":"10.1016/j.microc.2025.113135","DOIUrl":"10.1016/j.microc.2025.113135","url":null,"abstract":"<div><div>Feline herpesvirus type 1 (FHV-1) is the major pathogen of feline upper respiratory tract disease (URTD) and ocular disease, accounting for approximately 50 %–75 % of URTD in susceptible cats. However, similar clinical symptoms of URTD were present in the early stages of URTD, which makes it difficult to determine the pathogens with the naked eye. For that, there are a variety of nucleic acid detection methods have been established for FHV-1 determination in past years. However, they need complicated thermal cycling, specialized instruments, long detection time, brings false results risk and etc, which is not suitable for point-of-care testing. Therefore, this study developed a new nucleic acid detection method depending on recombinase aided amplification (RAA) (37 °C, 25 min), CRISPR/Cas13a <em>trans</em>-cleavage (37 °C, 20 min) and lateral flow strip (room temperature, 3 min). Firstly, the recombinant plasmid DNA, crRNA, and RAA primer pairs targeting the FHV-1 UL23 gene were designed and prepared. Secondly, the nucleic acid was extracted by lysis buffer and simple centrifugation. Thirdly, FHV-1 RAA nucleic acid amplification reaction was performed followed by CRISPR/Cas13a <em>trans</em>-cleavage report RNA and lateral flow strip visual detection. As a result, the present assay showed high specificity towards FHV-1 without cross-reactivity with other pathogens resulted in URTD and high sensitivity with the limit of detection at 0.768 copies/μL for the FHV-1 UL23 gene. Furthermore, clinical samples application was conducted for 24 clinical swab samples collected from cats with URTD using the present assay and the reference fluorescent quantitative PCR method simultaneously. As we expected, the results of the present assay were in exceptional concordance with the reference fluorescent quantitative PCR method. Accordingly, the present RAA-CRISPR/Cas13a-assisted lateral flow strip was developed for the rapid, ultra-sensitive, simple-operation, relible, visual and point-of-care testing of FHV-1, which ensures FHV-1’s rapid diagnosis in the early stages of infection and support a appropriate treatment on time in resource-limited field.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113135"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circulating tumour cells isolation in Asymmetrical meandering microchannel","authors":"Ahmed A. Ayash","doi":"10.1016/j.microc.2025.113132","DOIUrl":"10.1016/j.microc.2025.113132","url":null,"abstract":"<div><div>A microfluidic method is presented in the current work which allows full separation of circulating tumour cells (CTCs) from the blood biopsy of cancer patients. The method is based on using a microchannel with two separation zones. The first zone is meandering and equipped with seven identical electrodes. These electrodes generate dielectrophoretic forces that affect mostly the platelets and force them to separate first in this zone. This leaves only the target cells (CTCs) with RBCs and WBCs to flow through an expanded, curved section of the channel (second zone). This unique configuration allows generating intense hydrodynamic forces that can isolate CTCs completely. A numerical model is used to give insight into the cells separation and examine the performance of the proposed channel at different operating conditions. The computational results indicated that all CTCs can be separated when operating at a blood volume flow rate ranging from 10 to 20 µl/h. The two main electrical parameters (electrode voltage and AC current frequency) are also examined. The results showed that the voltage between 15 and 30 V and frequency between 20 and 70 kHz are the best ranges to isolate pure CTCs completely without including any blood cells (i.e. 100 % both separation efficiency and purity). Further, CTCs size is changed from 12 to 26 µm to mimic the effect of different CTC types and the results demonstrate clearly that with a single design various types of cancerous cells can be handled effectively.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113132"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study on the dynamic changes and relationships of volatile and semi-volatile compounds in flaxseed during the roasting procedure by using untargeted GC–MS combined with advanced chemometrics","authors":"Lu Han ,&nbsp;Long-He Wang ,&nbsp;Yan-Jin Wen ,&nbsp;Xue-Juan Zhang ,&nbsp;Ping-Ping Liu ,&nbsp;Xiang-Dong Zhao ,&nbsp;Qing-Xia Zheng ,&nbsp;Guo-Bi Chai ,&nbsp;Qi-Dong Zhang ,&nbsp;Yong-Jie Yu ,&nbsp;Yuanbin She","doi":"10.1016/j.microc.2025.113130","DOIUrl":"10.1016/j.microc.2025.113130","url":null,"abstract":"<div><div>Roasting flaxseed is an important stage in producing rich aromas. At present, characterizing the dynamically changed compounds is difficult. Here, a novel untargeted strategy for accurately and comprehensively analyzing volatile compounds such as flavors and semi-volatile compounds such as sugars and amino acids was provided, which is accomplished by integrating gas chromatography–mass spectrometry (GC–MS) with our newly developed automatic data analysis software AntDAS-GCMS. Samples at different roasting stages were collected, with the online released volatile compounds and inherent semi-volatile compounds analyzed simultaneously. Raw GC–MS data files were imported into AntDAS-GCMS to automatically perform compound resolution, registration, and identification. Finally, 53 volatile compounds and 83 semi-volatile compounds were screened and identified, whose dynamic variations during roasting were revealed. The relationship between semi-volatile and volatile compounds was studied in detail. The developed strategy provided a new solution for dynamic quality control during roasting.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"211 ","pages":"Article 113130"},"PeriodicalIF":4.9,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}