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MXene-based polyaniline/carbon nanotube composite for ultrasensitive chemiresistive ammonia sensors at room temperature 室温下用于超灵敏化学电阻氨传感器的mxene基聚苯胺/碳纳米管复合材料
IF 4.9 2区 化学
Microchemical Journal Pub Date : 2026-04-01 Epub Date: 2026-02-11 DOI: 10.1016/j.microc.2026.117356
Xueguo Li , Zaiping Chen , Songchao Bai , Mengmeng Dai , Jiwei Wang , Yongsheng Zhu , Wei Liu
{"title":"MXene-based polyaniline/carbon nanotube composite for ultrasensitive chemiresistive ammonia sensors at room temperature","authors":"Xueguo Li ,&nbsp;Zaiping Chen ,&nbsp;Songchao Bai ,&nbsp;Mengmeng Dai ,&nbsp;Jiwei Wang ,&nbsp;Yongsheng Zhu ,&nbsp;Wei Liu","doi":"10.1016/j.microc.2026.117356","DOIUrl":"10.1016/j.microc.2026.117356","url":null,"abstract":"<div><div>Developing high-performance room-temperature sensors for ammonia (NH<sub>3</sub>) detection remains a significant challenge due to the need for high sensitivity, selectivity, and stability under diverse environmental conditions. In this study, we present a novel gas sensor based on a polyaniline (PANI)-coated MXene carbon nanotube (CNT) composite (PANI@MXene CNTs), which effectively addresses these challenges. The unique core-shell architecture of PANI@MXene CNTs combines the high conductivity and surface area of MXene with the protonation behavior of PANI, leading to enhanced sensitivity and selectivity for NH<sub>3</sub> detection. The sensor demonstrates excellent performance, achieving a theoretical detection limit as low as 0.93 ppb for NH<sub>3</sub>, with rapid response and recovery times. It also exhibits high repeatability, long-term stability, and robust humidity resistance. The enhanced sensing capability arises from the synergistic effects between PANI and MXene, where PANI facilitates protonation, and MXene facilitates electron transfer. These interactions result in significant resistance changes upon NH<sub>3</sub> exposure, enabling precise detection across a wide concentration range (10 ppb to 50 ppm). Additionally, the sensor shows high selectivity for NH<sub>3</sub> over other gases, with negligible interference. This work highlights the potential of PANI@MXene CNTs as an effective diagnostic tool for NH<sub>3</sub>-related medical conditions, offering a promising solution for non-invasive, real-time gas sensing applications in both environmental and clinical settings.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"223 ","pages":"Article 117356"},"PeriodicalIF":4.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Smart dressings for wound monitoring and treatment: a review with focus on electrical stimulation therapy 用于伤口监测和治疗的智能敷料:以电刺激治疗为重点的综述
IF 4.9 2区 化学
Microchemical Journal Pub Date : 2026-04-01 Epub Date: 2026-02-11 DOI: 10.1016/j.microc.2026.117345
Xiaoping Lin , Li Yang , Ting Xu , Bowen Cheng , Hao Liu
{"title":"Smart dressings for wound monitoring and treatment: a review with focus on electrical stimulation therapy","authors":"Xiaoping Lin ,&nbsp;Li Yang ,&nbsp;Ting Xu ,&nbsp;Bowen Cheng ,&nbsp;Hao Liu","doi":"10.1016/j.microc.2026.117345","DOIUrl":"10.1016/j.microc.2026.117345","url":null,"abstract":"<div><div>Wound healing is a complex biological process involving multiple stages, such as cell proliferation, angiogenesis, and tissue remodeling. Chronic wounds, due to their intricate pathology and significant management difficulties, impose a substantial burden on patients and healthcare systems. Recent advances in materials science, microelectronics, and artificial intelligence have catalyzed the development of smart dressings, offering innovative solutions for wound care. This comprehensive review summarizes the development of multifunctional smart dressings. It highlights technologies for real-time monitoring of key wound biomarkers (e.g., pH, temperature, moisture, and oxygen levels) and provides a comparative analysis of drug delivery mechanisms, spanning passive diffusion, stimulus-responsive, and actively triggered release systems. The roles of physical therapies, including thermal, ultrasound, and phototherapeutic stimulation, are also discussed. Particular emphasis is placed on electrical stimulation (ES) therapy, owing to its unique mechanistic actions and strong compatibility with smart dressing platforms. This section details ES strategies, parameter optimization, the need for adaptive parameter adjustment guided by wound microenvironment feedback, and its synergistic potential with other therapies. Finally, the review identifies key challenges regarding system integration and clinical translation, while underscoring the promising future of smart dressings in enabling automated, personalized wound management.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"223 ","pages":"Article 117345"},"PeriodicalIF":4.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunocatalytic La₂O₃-Fe-Ni nanozyme detection of porcine pepsin in food matrices through ECL-colourimetric signal amplifications 免疫催化La₂O₃-Fe-Ni纳米酶检测食品基质中猪胃蛋白酶的ecl比色信号放大
IF 4.9 2区 化学
Microchemical Journal Pub Date : 2026-04-01 Epub Date: 2026-02-09 DOI: 10.1016/j.microc.2026.117323
Batrisyia Safwah Mohd Salleh, Fareeha Arshad, Koo Pey Ting, Minhaz Uddin Ahmed
{"title":"Immunocatalytic La₂O₃-Fe-Ni nanozyme detection of porcine pepsin in food matrices through ECL-colourimetric signal amplifications","authors":"Batrisyia Safwah Mohd Salleh,&nbsp;Fareeha Arshad,&nbsp;Koo Pey Ting,&nbsp;Minhaz Uddin Ahmed","doi":"10.1016/j.microc.2026.117323","DOIUrl":"10.1016/j.microc.2026.117323","url":null,"abstract":"<div><div>The presence of porcine pepsin in food items is widespread globally. Despite the prohibition of such ingredients in food products within religious and vegan communities, as well as for medical concerns, there is still uncertainty in determining their origin in food products. The failure of certain businesses in the food industry to clearly label porcine pepsin-containing food products necessitates an intervention that this study can provide. In the present study, we report dual-mode detection of porcine pepsin using a novel La<sub>2</sub>O<sub>3</sub>-Fe-Ni nanozyme in the developed immunosensor. The nanozyme exhibited enhanced peroxidase-mimicking activity, which collaboratively boosted the electrochemiluminescence signal generated. The immunosensor achieved a significantly low detection limit for porcine pepsin in both detection modes. The additional integration of machine learning enables precise and accurate porcine pepsin prediction. The practical application of the immunosensor was validated by spiking food samples, which indicated promising recoveries.</div></div><div><h3>Novelty statement and impact</h3><div>This study assembled a novel La<sub>2</sub>O<sub>3</sub>-Fe-Ni nanozyme for the colourimetric-electrochemiluminescence immunodetection of porcine pepsin in food samples. The immunocomplexes formed between anti-pepsin antibody and the target are separated through magnetism, which allows only the specific immunocomplexes to be recovered and utilised in the detection modes, omitting the need for blocking agents. The immunocomplexes were directly drop-cast onto the electrode and wells of a microtiter plate, which bypasses the time-consuming washing steps and layer-by-layer incubation of nanozyme, target, and antibody; while reducing noise signals from blocking agents. Additionally, the nanozyme exhibited remarkable peroxidase-mimicking activity and kinetic parameters, surpassing those of horseradish peroxidase and previously published literature on nanozymes. The kinetic parameters of the counter nanomaterials were also established, which had not been previously evaluated. The wide linear range covered and low detection limit obtained in both methods are favourable, as there are limited studies on porcine pepsin detection, and they have only employed a single detection method with a high limit of detection value and a narrow detection range. Furthermore, machine learning is used to predict various concentrations of porcine pepsin from the obtained datasets.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"223 ","pages":"Article 117323"},"PeriodicalIF":4.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advances in carbon-based nanomaterials for biosensing and bioanalytical applications: from CNTs to graphene, MXenes, MOFs, and MIPs 用于生物传感和生物分析的碳基纳米材料的进展:从碳纳米管到石墨烯、MXenes、mof和mip
IF 4.9 2区 化学
Microchemical Journal Pub Date : 2026-04-01 Epub Date: 2026-02-10 DOI: 10.1016/j.microc.2026.117315
Bharathi Natarajan , Palanisamy Kannan , Govindhan Maduraiveeran
{"title":"Advances in carbon-based nanomaterials for biosensing and bioanalytical applications: from CNTs to graphene, MXenes, MOFs, and MIPs","authors":"Bharathi Natarajan ,&nbsp;Palanisamy Kannan ,&nbsp;Govindhan Maduraiveeran","doi":"10.1016/j.microc.2026.117315","DOIUrl":"10.1016/j.microc.2026.117315","url":null,"abstract":"<div><div>Carbon-based nanomaterials (CNMs) have driven significant progress in various biological fields, including diagnostics, bioimaging, and therapeutic monitoring. By integrating carbon-based nanostructures with biological components, researchers have enhanced biointerface sensitivity, flexibility, and functionality, enabling precise diagnostic techniques and improving therapeutic outcomes. However, optimizing these bio-hybrid systems requires a deep understanding of the interactions between material structure, biosensing activity, and biosafety. These insights are crucial for designing nanomaterials that meet the specific needs of clinical and biomedical applications while ensuring both safety and performance. This review explores the advancements in CNMs-enabled biosensing, with a specific focus on various types, including graphene, carbon nanotubes (CNTs), graphyne, and hybrid carbon-bio systems. We examine the relationship between nanomaterial properties and biological systems, highlighting the role of these different frameworks in enhancing biosensing capabilities and therapeutic applications. Topics include efficient probe design, surface functionalization, adsorption dynamics, and optimization of carbon-bio-interfaces to enhance signal transduction and bio-recognition. In addition, we address key analytical metrics including sensitivity and specificity, while highlighting challenges such as reproducibility, in-vivo applicability, and long-term biosafety. Emerging trends and future directions in bioanalytical research are also discussed.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"223 ","pages":"Article 117315"},"PeriodicalIF":4.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
AI-driven energy material design and battery life improvement methods for wearable sports devices 面向可穿戴运动设备的ai驱动能源材料设计及电池寿命提升方法
IF 4.9 2区 化学
Microchemical Journal Pub Date : 2026-04-01 Epub Date: 2026-02-06 DOI: 10.1016/j.microc.2026.117294
Dongdong Zheng , Hui Li , Hongqiao Yan
{"title":"AI-driven energy material design and battery life improvement methods for wearable sports devices","authors":"Dongdong Zheng ,&nbsp;Hui Li ,&nbsp;Hongqiao Yan","doi":"10.1016/j.microc.2026.117294","DOIUrl":"10.1016/j.microc.2026.117294","url":null,"abstract":"<div><div>This study examines the design and endurance enhancement of energy materials for AI-driven wearable sports devices by systematically comparing the performance characteristics of lithium batteries, supercapacitors, graphene batteries, and sodium-ion batteries. An energy efficiency optimization model based on graph neural networks is proposed. Experimental results show that the model extends battery life by 45%–75% while restricting energy loss to 70 mW·h, which is nearly 50% lower than that of traditional battery management systems. Reinforcement learning algorithms further extend battery lifespan by up to 35%, albeit at a computational complexity of 10,000 FLOPS. With kinetic energy recovery efficiency at 50%, device operation time increases from 3 h to over 10 h. Energy consumption comparisons reveal that smart bands, with a standby power of 20–30 mW, can operate for more than 200 h, whereas smart sports glasses operating in GPS mode consume 90–120 mW and sustain operation for only about 30 h. By integrating material properties with energy management strategies using AI technologies, this research provides a quantitatively supported pathway for the prolonged operation of wearable devices.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"223 ","pages":"Article 117294"},"PeriodicalIF":4.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pharmaceutical pollutant ornidazole sensing at mesoporous spinel cobalt cerium oxide and graphene nanoplatelets modified electrode in environmental water and fish samples 介孔尖晶石钴铈氧化物和石墨烯纳米片修饰电极对环境水和鱼样品中药物污染物奥硝唑的传感
IF 4.9 2区 化学
Microchemical Journal Pub Date : 2026-04-01 Epub Date: 2026-02-13 DOI: 10.1016/j.microc.2026.117311
Viswa Muthiah , Venkataraman Dharuman
{"title":"Pharmaceutical pollutant ornidazole sensing at mesoporous spinel cobalt cerium oxide and graphene nanoplatelets modified electrode in environmental water and fish samples","authors":"Viswa Muthiah ,&nbsp;Venkataraman Dharuman","doi":"10.1016/j.microc.2026.117311","DOIUrl":"10.1016/j.microc.2026.117311","url":null,"abstract":"<div><div>Presence of pharmaceutical pollutants greater than the permissible concentration in the environmental water poses significant health problems and affect environmental health underscoring the development of sensitive techniques to monitor. This study synthesized mesoporous spinel cobalt cerium oxide (CoCe<sub>2</sub>O<sub>4</sub>) using the simple hydrothermal method and anchored with graphene nanoplatelets through physical adsorption (CoCe<sub>2</sub>O<sub>4</sub>/Gr NP). Characterization was done using X-ray diffraction (XRD), Raman spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), high resolution transmission electron microscopy (HR-TEM), field-emission scanning electron microscopy (FE-SEM) and Brunauer-Emmett-Teller (BET). Studies confirmed spherical and mesoporous structure of the composite. The electrochemical study in the presence of [Fe(CN)<sub>6</sub>]<sup>3−/4-</sup> reveals the highest redox current with lower ∆E<sub>p</sub> (77 mV) for the CoCe<sub>2</sub>O<sub>4</sub>/Gr compared to the Co<sub>3</sub>O<sub>4</sub> (∆E<sub>p</sub>: 180 mV) and CoCe<sub>2</sub>O<sub>4</sub> (∆E<sub>p</sub>: 221 mV) and Gr NP (∆E<sub>p</sub>: 83 mV) modified electrodes due to the improved surface area and conductivity. In the presence of ODZ, the sensor showed highest and very sharp reduction peak (<em>i</em><sub><em>pa</em></sub>: −22.95 μA) at the lowest overpotential −0.574 V with the sensitivity of 0.1924 μA μM<sup>−1</sup> cm<sup>−2</sup>, limit of detection (LOD) of 0.0639 μM, limit of quantification (LOQ) of 0.2128 μM. A linear detection range from 125 nM to 1.674 mM with good reproducibility, renewability and stability was obtained. Practical application was demonstrated using pharmaceutical tablets, environmental water samples (lake, well, and tap water), fish samples, and achieved high recovery rates.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"223 ","pages":"Article 117311"},"PeriodicalIF":4.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147386244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A biothiol-activated fluorescent probe for hemoglobin dynamic monitoring 用于血红蛋白动态监测的生物硫醇激活荧光探针
IF 4.9 2区 化学
Microchemical Journal Pub Date : 2026-04-01 Epub Date: 2026-02-09 DOI: 10.1016/j.microc.2026.117328
Jing Zhang , Yong-Bin Liu , Hong-Kai Bian , Xin-Yu Pan , Xiang Ding , Qing Wang , Wen-Bo Liu
{"title":"A biothiol-activated fluorescent probe for hemoglobin dynamic monitoring","authors":"Jing Zhang ,&nbsp;Yong-Bin Liu ,&nbsp;Hong-Kai Bian ,&nbsp;Xin-Yu Pan ,&nbsp;Xiang Ding ,&nbsp;Qing Wang ,&nbsp;Wen-Bo Liu","doi":"10.1016/j.microc.2026.117328","DOIUrl":"10.1016/j.microc.2026.117328","url":null,"abstract":"<div><div>Abnormalities in hemoglobin (Hb) concentration function as crucial biomarkers for a variety of pathological conditions. Although several methods are available for highly sensitive detection of Hb, there is still a lack of efficient tools for dynamic monitoring of Hb in specific pathological environments. Herein, we developed a novel fluorescent probe (TDA) for biothiol-mediated dynamic monitoring of Hb. TDA exhibited responsiveness to various biothiols, and it showed a stronger responsiveness to glutathione (GSH). Moreover, while TDA itself did not demonstrate significant responsiveness to proteins and DNA, the biothiol-activated TDA (i.e., TDA-OH) displayed favorable selective fluorescence “turn-off” responsiveness to bovine hemoglobin (BHb). Both TDA and TDA-OH possessed good BHb binding capability, and common commercial drugs had minimal impact on this binding. Non-covalent interactions played a pivotal role in stabilizing the interaction between BHb and TDA/TDA-OH. As a result of this stability, even when TDA was activated by biothiol, the resulting TDA-OH could still remain stably embedded within BHb, with its fluorescence in a quenched state. When BHb was damaged, the quenched fluorescence of TDA-OH recovered, resulting in a noticeable fluorescence enhancement. This cascade response property thus endows TDA with potential for specific and dynamic monitoring of Hb in a pathological environment with overexpressed biothiol.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"223 ","pages":"Article 117328"},"PeriodicalIF":4.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146147275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nanostructured zinc chromate decorated on halloysite nanotubes for high-performance detection of dimetridazole in food samples 高岭土纳米管修饰纳米结构铬酸锌用于食品样品中二甲硝唑的高效检测
IF 4.9 2区 化学
Microchemical Journal Pub Date : 2026-04-01 Epub Date: 2026-02-10 DOI: 10.1016/j.microc.2026.117291
Loganathan Vagismathi , Sea-Fue Wang
{"title":"Nanostructured zinc chromate decorated on halloysite nanotubes for high-performance detection of dimetridazole in food samples","authors":"Loganathan Vagismathi ,&nbsp;Sea-Fue Wang","doi":"10.1016/j.microc.2026.117291","DOIUrl":"10.1016/j.microc.2026.117291","url":null,"abstract":"<div><div>The extensive application of veterinary drugs and agrochemicals has emerged as a pressing global issue due to their persistence in the environment and long-term implications for human health and ecosystems. Dimetridazole (DZ), a nitroimidazole compound widely utilised in animal husbandry and poultry, has also drawn substantial concern due to its persistence, bioaccumulation, and toxicological implications, including mutagenic and carcinogenic effects. To meet the demand for effective monitoring strategies, this work proposes a Zinc chromate and halloysite nanotube-based nanocomposite as an electrocatalytic sensing platform for detecting DZ. The spinel ZnCr<sub>2</sub>O<sub>4,</sub> which exhibits high physicochemical stability, is integrated with HNTs, which provide a high surface area to the nanocomposite. The resulting ZnCr<sub>2</sub>O<sub>4</sub>/HNT electrode demonstrates enhanced electrochemical performance relative to pristine ZnCr<sub>2</sub>O<sub>4</sub>, attributable to accelerated charge transfer, greater exposure of active sites, and improved interfacial interactions between DZ and the nanocomposite surface. The ZnCr<sub>2</sub>O<sub>4</sub>/HNT/GCE sensor achieves a detection limit of 0.0036 μM and a linear concentration range of 0.1–736.4 μM, demonstrating excellent sensing performance for the detection of DZ. Furthermore, the as-constructed sensor exhibits excellent reproducibility and selectivity, with recoveries of 98–99% with relative standard deviation (RSD) values below 4%. The ZnCr<sub>2</sub>O<sub>4</sub>/HNT/GCE sensor is applied to real sample analysis, including milk and tomato, confirming its practical applicability for food safety and environmental monitoring.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"223 ","pages":"Article 117291"},"PeriodicalIF":4.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ratiometric dual-mode ECL–SERS biosensing of miR-21 via PER-primed CRISPR/Cas12a on CsPbBr₃@COF-LZU1@Au 在CsPbBr₃@COF-LZU1@Au上通过per引物CRISPR/Cas12a对miR-21进行比例双模ECL-SERS生物传感
IF 4.9 2区 化学
Microchemical Journal Pub Date : 2026-04-01 Epub Date: 2026-02-11 DOI: 10.1016/j.microc.2026.117278
Zhengfang Liang , Jin Chen , Guoli Zuo , Junhong Li , Jihua Wei , Yuanxun Gong , Haozhen Ren , Qianli Tang , Kai Zhang , Xianjiu Liao
{"title":"Ratiometric dual-mode ECL–SERS biosensing of miR-21 via PER-primed CRISPR/Cas12a on CsPbBr₃@COF-LZU1@Au","authors":"Zhengfang Liang ,&nbsp;Jin Chen ,&nbsp;Guoli Zuo ,&nbsp;Junhong Li ,&nbsp;Jihua Wei ,&nbsp;Yuanxun Gong ,&nbsp;Haozhen Ren ,&nbsp;Qianli Tang ,&nbsp;Kai Zhang ,&nbsp;Xianjiu Liao","doi":"10.1016/j.microc.2026.117278","DOIUrl":"10.1016/j.microc.2026.117278","url":null,"abstract":"<div><div>We report a single-electrode, dual-mode biosensor that co-integrates anodic electrochemiluminescence (ECL) and surface-enhanced Raman scattering (SERS) on CsPbBr₃@COF-LZU1@Au nano-hybrids. The perovskite core provides bright, water-stable ECL under tri-n-propylamine (TPrA), the ultrathin COF shell offers porous confinement and chemical anchoring, and peripheral Au nanodots create electromagnetic hot spots and robust Au<img>S linkages. A ferrocene-tagged hairpin (Fc-HP) positions Fc inside the sub-nanometer to few-nanometer (∼0.5–3 nm) Au–COF–perovskite nanogap to impose a deep OFF baseline by distance-dependent quenching. Target recognition is amplified upstream by primer-exchange reaction (PER), which primes CRISPR/Cas12a for collateral trans-cleavage of surface DNA, withdrawing/distalizing Fc to restore ECL and attenuate the Fc SERS signature in a synchronized, target-dependent manner. Acquisition is ordered SERS-first/ECL-second to avoid interfacial perturbation. Under optimized conditions (PER 80 min, Cas12a 60 nM, on-electrode cleavage 45 min, TPrA 70 mM), synthetic miR-21 yields nine-decade log-linear behavior from 1 aM to 1 nM: normalized ECL increases with log<sub>10</sub>C, SERS(Fc) decreases with log<sub>10</sub>C, and the ratiometric index R = I<sub>ECL</sub>/I<sub>SERS(Fc)</sub> is also linear across the same range. IUPAC LODs are 1.71 aM (ECL), 2.14 aM (SERS(Fc)), and 1.51 aM (ratiometric). The platform shows high selectivity against noncognate/mismatched sequences, excellent reproducibility (inter-electrode RSD ≈ 3%), and practical stability (≈92–96% signal retention over 7 days at 4 °C). In real matrices, endogenous miR-21 in total RNA follows the expected trend MCF-7 &gt; HeLa &gt; HEK293, with accurate spike-recovery using matrix-matched calibration. The modular PER→Cas12a recognition and the co-engineered nanogap transducer enable sensitive, cross-validated quantification and are readily adaptable to other microRNAs and nucleic-acid biomarkers.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"223 ","pages":"Article 117278"},"PeriodicalIF":4.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Flower-like Cu/Ag nanozyme as oxidase-mimic for the colorimetric detection of hydroquinone 花状铜/银纳米酶作为氧化酶模拟物比色法检测对苯二酚
IF 4.9 2区 化学
Microchemical Journal Pub Date : 2026-04-01 Epub Date: 2026-02-14 DOI: 10.1016/j.microc.2026.117382
Yi Liu , Xingxing Ouyang , Hongfen Yang , Dan Yang , Jia Ge , Yu Liu , Ren Cai
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