Bioelectrochemistry最新文献

Corrigendum to "Molecular monolayers on silicon as substrates for biosensors" [Bioelectrochem. 80(1) (2010) 17-25]. 硅上的分子单层作为生物传感器的基底"[Bioelectrochem.

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-01 Epub Date: 2024-11-17 DOI: 10.1016/j.bioelechem.2024.108849

L Touahir, P Allongue, D Aureau, R Boukherroub, J-N Chazalviel, E Galopin, A C Gouget-Laemmel, C Henry de Villeneuve, A Moraillon, J Niedziółka-Jönsson, F Ozanam, J Salvador Andresa, S Sam, I Solomon, S Szunerits

引用次数: 0

Corrigendum to "Analysis of electromagnetic response of cells and lipid membranes using a model-free method" [Bioelectrochemistry 152 (2023) 108444]. 对 "利用无模型方法分析细胞和脂膜的电磁响应"[生物电化学 152 (2023) 108444] 的更正。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-01 Epub Date: 2024-11-02 DOI: 10.1016/j.bioelechem.2024.108841

Yingxian Lu, Xiaping Tang, Yanyu Zhao, Tianyu Jiang, Jiayao Zhou, Xiaofei Wang, Bing Huang, Lingyu Liu, Hu Deng, Yujing Huang, Yigong Shi

引用次数: 0

An electrochemical aptasensor based on bimetallic carbon nanocomposites AuPt@rGO for ultrasensitive detection of adenosine on portable potentiostat.

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-22 DOI: 10.1016/j.bioelechem.2025.108906

Zheng Gong, Hongli Zhao, Yi Mao, Fangfang Zhou, Zehui Shi, Minbo Lan

{"title":"An electrochemical aptasensor based on bimetallic carbon nanocomposites AuPt@rGO for ultrasensitive detection of adenosine on portable potentiostat.","authors":"Zheng Gong, Hongli Zhao, Yi Mao, Fangfang Zhou, Zehui Shi, Minbo Lan","doi":"10.1016/j.bioelechem.2025.108906","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2025.108906","url":null,"abstract":"Adenosine plays a crucial role in the cardiovascular and nervous systems of living organisms. Excessive adenosine can lead to arrhythmias or heart failure, making the accurate detection of adenosine highly valuable. Given the widespread use of sensors for detecting small molecules, we propose a sensitive electrochemical aptasensor for adenosine detection in this study. The aptasensor utilized reduced graphene oxide (rGO) as the base material, and its performance was further enhanced by loading gold-platinum nanoparticles (AuPt@rGO) to improve the current response. The AuPt@rGO composite was synthesized using a simple process and was modified onto screen-printed electrodes (SPE) fabricated in the laboratory. This modification increased the effective area of the working electrode, providing more contact sites and enhancing sensitivity for adenosine detection. As a result, the sensor exhibited excellent performance in selectivity, sensitivity, and stability, with a linear response range for adenosine from 10 nM to 10 μM and a detection limit of 1.28 nM (n = 3). Furthermore, successful detection of adenosine levels in real samples was achieved using a portable potentiostat, demonstrating excellent recovery rates (95.99 %-103.4 %). These results indicate the aptasensor's significant potential for future applications in field analysis and medical diagnostics.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108906"},"PeriodicalIF":4.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytochrome P450 electrochemical biosensors transforming in vitro metabolism testing - Opportunities and challenges.

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-21 DOI: 10.1016/j.bioelechem.2025.108913

Carina S P Vieira, Marcela A Segundo, Alberto N Araújo

{"title":"Cytochrome P450 electrochemical biosensors transforming in vitro metabolism testing - Opportunities and challenges.","authors":"Carina S P Vieira, Marcela A Segundo, Alberto N Araújo","doi":"10.1016/j.bioelechem.2025.108913","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2025.108913","url":null,"abstract":"The ability of the living world to flourish in the face of constant exposure to dangerous chemicals depends on the management ability of a widespread group of enzymes known as heme-thiolate monooxygenases or cytochrome P450 superfamily. About three-quarters of all reactions determining the metabolism of endogenous compounds, of those carried in foods, of taken drugs, or even of synthetic chemicals discarded into the environment depend on their catalytic performance. The chromatographic and (photo)luminometric methods routinely used as predictive and analytical tools in laboratories have significant drawbacks ranging from limited shelf-life of reagents, use of synthetic substrates, laborious and tedious procedures for highly sensitive detection. In this review, alternative electrochemical biosensors using the cytochrome P450 enzymes as bio-element are emphasized in their main aspects as well regarding their implementation and usefulness. Despite the various schemes proposed for the implementation, reports on real applications are scant for several reasons, including low reaction rates, broad substrate specificity, uncoupling reactions occurrence, and the need for expensive electron transfer partners to promote electron transfer. Finally, the prospect for future developments is introduced, focusing on integrating miniaturized systems with electrochemical techniques, alongside optimizing enzyme immobilization methods and electrode modifications to improve enzymatic stability and enhance sensor reliability. This progress represents a crucial step towards the creation of portable biosensors that mimic human physiological responses, supporting the precision medicine approach.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108913"},"PeriodicalIF":4.8,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time prediction of heparin concentration in blood extracorporeal circulation by relaxation time distribution (RTD).

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-20 DOI: 10.1016/j.bioelechem.2025.108912

Soichiro Ueno, Daisuke Kawashima, Katsuhiro Matsuura, Hiromichi Obara, Ryou Tanaka, Masahiro Takei

{"title":"Real-time prediction of heparin concentration in blood extracorporeal circulation by relaxation time distribution (RTD).","authors":"Soichiro Ueno, Daisuke Kawashima, Katsuhiro Matsuura, Hiromichi Obara, Ryou Tanaka, Masahiro Takei","doi":"10.1016/j.bioelechem.2025.108912","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2025.108912","url":null,"abstract":"Heparin concentration c in a blood extracorporeal circulation has been real-timely predicted based on the relaxation strength Δεm at relaxation frequency fm extracted by relaxation time distribution (RTD). The simulated extracorporeal circulation was conducted to optimize the number of Δεm for the prediction of c using the porcine whole blood (WB) and low-leukocyte and -platelet blood (LLPB) under the condition of the gradual increment of c from 0 to 8 U/mL with constant flow rate and blood temperature. The experimental results show that among the three relaxation strengths Δε1, Δε2 and Δε3 (in ascending order of frequency), Δε2 at f2 = 5.2 ∼ 6.2 MHz and Δε3 at f3 = 42 ∼ 50 MHz were correlated to c. The Δε3 was decreasing with increasing c in both cases, which was influenced by the plasma macromolecular concentrations, while the Δε2 was increased with increasing c in WB case but was hardly changed in LLPB case because the Δε2 is influenced by the blood cell concentrations and the shape changes of blood cell membranes. Heparin concentration c is estimated by the linear regression formula cPRE=a1(Δε2-Δε2c=0)+a2(Δε3-Δε3c=0) (a1 = -0.991, a2 = -0.123) within the mean absolute percentage error (MAPE) of 0.291.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108912"},"PeriodicalIF":4.8,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of micro-nanostructured film with antibacterial, anticorrosive and thermal conductivity properties on copper surface.

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-15 DOI: 10.1016/j.bioelechem.2025.108905

Li Lai, Muqiu Xia, Mengyu Fu, Yuanyuan Gao, Jiahao Sun, Guangzhou Liu, Shiqiang Chen

引用次数: 0

Print-Light-Synthesis of ruthenium oxide thin film electrodes for electrochemical sensing applications.

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-15 DOI: 10.1016/j.bioelechem.2025.108909

Stefano Gianvittorio, Marco Malferrari, Horst Pick, Stefania Rapino, Andreas Lesch

{"title":"Print-Light-Synthesis of ruthenium oxide thin film electrodes for electrochemical sensing applications.","authors":"Stefano Gianvittorio, Marco Malferrari, Horst Pick, Stefania Rapino, Andreas Lesch","doi":"10.1016/j.bioelechem.2025.108909","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2025.108909","url":null,"abstract":"Print-Light-Synthesis (PLS) combines the inkjet printing of a ruthenium precursor ink with the simultaneous photo-induced generation of ruthenium oxide films. During PLS, inkjet-printing generates on conductive as well as insulating substrates micrometer-thin reaction volumes that contain with high precision defined precursor loadings. Upon direct UV light irradiation, the Ru precursor converts to RuO2 while all other ink components escape in the gas phase. No post PLS processes are required, and the as-obtained RuO2 films can be immediately used as electrochemical devices. Two-dimensional RuO2 patterns with micrometric resolution and highly-controlled ruthenium loadings (few µg/cm2) are realized. Thin RuO2 films are generated on insulating substrates, such as polyimide, as well as individual RuO2 particles on conductive substrates, such as graphene layers. The RuO2 films are characterized by electron microscopy and spectroscopic techniques. The sensoristic applicability of the PLS-RuO2 electrodes is demonstrated by potentiometric pH sensing in cell cultures and amperometric detection of L-cysteine. For pH sensing the RuO2 film electrodes show Nernstian sensitivity. L-cysteine detection of RuO2-modified graphene electrodes showed an electrocatalytical effect and resulted in the possibility of selectively detecting L-Cysteine also in presence of the interfering compound uric acid.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108909"},"PeriodicalIF":4.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced detection of Cystatin C for predicting adverse outcomes in gestational diabetes mellitus using a point-of-care immunosensor. 利用即时免疫传感器增强胱抑素C检测，预测妊娠期糖尿病的不良结局。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-13 DOI: 10.1016/j.bioelechem.2025.108907

Ya Jin, Yongmei Chen, Xiaoqin Li, Songjun Han, Lei Mou, Nan Li

{"title":"Enhanced detection of Cystatin C for predicting adverse outcomes in gestational diabetes mellitus using a point-of-care immunosensor.","authors":"Ya Jin, Yongmei Chen, Xiaoqin Li, Songjun Han, Lei Mou, Nan Li","doi":"10.1016/j.bioelechem.2025.108907","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2025.108907","url":null,"abstract":"Cystatin C (Cys-C) is emerging as a critical biomarker for assess gestational diabetes mellitus (GDM), a condition that significantly impacts maternal and fetal health. In this study, we developed a novel label-free electrochemical immunosensor designed for point-of-care applications, offering lower reagent consumption and rapid detection of Cys-C in pregnant women with GDM. Compared to traditional enzyme-linked immunosorbent assays (ELISA), the sensor demonstrates enhanced sensitivity, reduced reagent usage, and faster detection. In a cohort study involving 150 pregnant women with GDM and 150 healthy controls, serum Cys-C levels were analyzed using the developed sensor. Serum samples were collected and analyzed for Cys-C levels using our immunosensor. Serum Cys-C levels were significantly elevated in GDM patients compared to controls (P < 0.05), and higher levels were observed in GDM complicated by pregnancy-induced hypertension (PIH) and fetal growth restriction (FGR). Furthermore, elevated Cys-C levels were positively correlated with adverse pregnancy outcomes, including premature birth, fetal distress, and cesarean section (P < 0.05). This study underscores the potential of Cys-C as a reliable biomarker for GDM and highlights the advantages of our novel sensor for quick, accurate, and scalable GDM diagnosis and management.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108907"},"PeriodicalIF":4.8,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conductive and flexible gold-coated polylactic acid nanofiber-based electrochemical aptasensor for monitoring cortisol level in sweat and saliva. 用于监测汗液和唾液中皮质醇水平的导电柔性金包覆聚乳酸纳米纤维电化学适体传感器。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-13 DOI: 10.1016/j.bioelechem.2025.108908

Mkliwa Koumbia, Takai Madoka

{"title":"Conductive and flexible gold-coated polylactic acid nanofiber-based electrochemical aptasensor for monitoring cortisol level in sweat and saliva.","authors":"Mkliwa Koumbia, Takai Madoka","doi":"10.1016/j.bioelechem.2025.108908","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2025.108908","url":null,"abstract":"Conductive nanofibers can exhibit excellent mechanical properties such as flexibility, elasticity, porosity, large surface area-to-volume ratio, etc making them suitable for a wide range of applications including biosensor development. Their large surface area provides more active sites for immobilization of large amount of bioreceptors enabling more interaction sites with the target analytes, enhancing sensitivity and detection capabilities. However, engineering conductive nanofibers with such excellent properties is challenging limiting their effective deployment for intended applications. In this research, we propose a novel approach for easy fabrication of highly conductive and flexible nanofiber leveraging the electrospinning, electroless deposition and have applied it to cortisol monitoring; a common biomarker for stress which is often quantified through enzyme-linked immunoassays using blood or saliva samples. By adopting the nanofiber sheet as a transducer for aptamer immobilization and cortisol sensing our developed biosensor was able to detect cortisol in buffer, artificial saliva, and artificial sweat within five minutes, from 10 pg/mL to 10 µg/mL (27.59 pM to 27.59 µM) with a low detection limit of 1 pg/ml (2.76 pM). The Au-coated PLA nanofiber-based electrochemical biosensor's flexibility allows for compact manufacturing, rendering it an optimal choice for integration into point-of-care testing and wearable systems.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108908"},"PeriodicalIF":4.8,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized detection of calcium ion in serum using constant potential coulometry with metastable liquid-liquid contact doping enhanced PEDOT: PSS ink. 亚稳液-液接触掺杂增强PEDOT: PSS墨水恒电位库仑法优化血清钙离子检测。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-10 DOI: 10.1016/j.bioelechem.2025.108903

Suyun Wei, Jie Zhao, Shaojun Ke, Mingjia Zou, Qingze Han, Guofeng Cui

{"title":"Optimized detection of calcium ion in serum using constant potential coulometry with metastable liquid-liquid contact doping enhanced PEDOT: PSS ink.","authors":"Suyun Wei, Jie Zhao, Shaojun Ke, Mingjia Zou, Qingze Han, Guofeng Cui","doi":"10.1016/j.bioelechem.2025.108903","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2025.108903","url":null,"abstract":"Highly stable calcium ion selective electrodes (Ca2+-ISEs) were developed by drop-casting a layer of poly(3,4-ethylenedioxythiophene): polystyrene sulfonate (PEDOT: PSS) as an ion-to-electron transfer layer onto Au electrode. The conductive PEDOT: PSS ink was prepared using a metastable liquid-liquid contact (MLLC) doping method, which induced phase separation, removed excess PSS, and significantly enhanced charge transfer kinetics and conductivity. The resulting Ca2+-ISEs exhibited excellent electrochemical performance. Potentiometric studies revealed a significant sensitivity of 33.1 ± 0.98 mV/decade (N = 3) with a high potential stability of 3.16 ± 2.53 μV/h. Importantly, Ca2+-ISEs combined with the constant potential coulometry method, the lower detection limit was optimized to 8.527 × 10-8 M (LOD = 3σ/s, N = 3). The performance of the Ca2+-ISE system was evaluated in inactivated fetal bovine serum using constant potential coulometry, demonstrating the highest measurement accuracy compared to potentiometric and chronoamperometric. The enhanced PEDOT: PSS-MLLC based Ca2+-ISEs combined with the constant potential coulometry method developed in this research demonstrate considerable potential for clinical applications in blood ion analysis.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108903"},"PeriodicalIF":4.8,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0