BioelectrochemistryPub Date : 2025-06-18DOI: 10.1016/j.bioelechem.2025.109027
Yanan Pu , Fan Feng , Yue Hou , Su Hou , Zihao Guo , Congrui Zhu , Shougang Chen
{"title":"Impact of yeast extract on bacterial metabolism and nickel microbiologically influenced corrosion: Insights into medium optimization and biofilm electron transfer mechanism","authors":"Yanan Pu , Fan Feng , Yue Hou , Su Hou , Zihao Guo , Congrui Zhu , Shougang Chen","doi":"10.1016/j.bioelechem.2025.109027","DOIUrl":"10.1016/j.bioelechem.2025.109027","url":null,"abstract":"<div><div>Yeast extract (YE) serves as a complex nutrient source and plays a pivotal role in the formation and development of microbial biofilms. This work elucidates the critical role of YE in modulating the metabolic activity of <em>Desulfovibrio vulgaris</em>, characteristics of passive films, and the associated microbiologically influenced corrosion (MIC) behavior of nickel (Ni). The presence of YE suppresses corrosion processes linked to extracellular electron transfer (EET) by reducing the necessity for <em>D. vulgaris</em> to directly extract electrons from Ni. In the absence of YE, a greater number of <em>D. vulgaris</em> cells adhere to the Ni surface, forming biofilms with an increased reliance on EET from Ni, thereby exacerbating localized corrosion. This is evidenced by increased weight loss, deeper pitting, and elevated localized corrosion current density, establishing a clear correlation between YE availability and the mitigation of EET-mediated MIC. Meanwhile, YE mitigates EET-driven corrosion by regulating the biofilm structure, facilitating the formation of a protective layer, and modifying the passive film on Ni. A key implication of this work is the reconsideration of YE as a universal nutrient in MIC research, emphasizing the need for caution when using YE in MIC studies that focus on EET-driven mechanisms.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109027"},"PeriodicalIF":4.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioelectrochemistryPub Date : 2025-06-17DOI: 10.1016/j.bioelechem.2025.109035
Licong Jia , Kai Kang , Wen Yan , Yan Jin , Kejia Xu , Jingyi Wang , Yipeng Wang , Meng Jiang , Wei Yuan , Shipeng Liu , Lingmei Niu
{"title":"17β-Estradiol point-of-care test by commercial pregnancy test strips based on target-triggered CRISPR/Cas12a cleavage activity","authors":"Licong Jia , Kai Kang , Wen Yan , Yan Jin , Kejia Xu , Jingyi Wang , Yipeng Wang , Meng Jiang , Wei Yuan , Shipeng Liu , Lingmei Niu","doi":"10.1016/j.bioelechem.2025.109035","DOIUrl":"10.1016/j.bioelechem.2025.109035","url":null,"abstract":"<div><div>17β-Estradiol (E2) serves as both a reproductive accelerator and growth promoter, yet its uncontrolled application induces severe endocrine dysfunction. Point-of-care testing (POCT) has emerged as a promising analytical platform for on-site E2 quantification, due to its inherent advantages in terms of sensitive, specific, cost-effective, and instrument-free. We developed a novel strategy combining the high collateral cleavage activity of CRISPR/Cas12a with the strong amplification power of dual-cycle reaction, translating target presence into a colorimetric signal on pregnancy test strip (PTS). In the presence of E2, the dual-cycle amplification was initiated to produce HP2–HP3 (Hairpin DNA 2–Hairpin DNA 3) duplexes. These duplexes then bound to the Cas12a-crRNA complex, activating its trans-cleavage activity. The activated Cas12a subsequently cleaved the ssDNA linkers in the MBs (magnetic beads)-ssDNA (single-stranded DNA)-hCG (human chorionic gonadotropin) conjugates, releasing hCG for quantitative E2 detection through strip color intensity measurement. By integrating CRISPR/Cas12a's high cleavage efficiency with dual-cycle amplification, a visible biosensor was developed, with a linear range of 1.0 × 10<sup>−1</sup>–200.0 pM and a detection limit of 0.0403 pM for E2. In conclusion, an E2 detection platform characterized by prominent sensitivity and convenience was established for monitoring spiked E2 in milk and urine, providing a significant reference for the POCT detection of other targets.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109035"},"PeriodicalIF":4.8,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioelectrochemistryPub Date : 2025-06-17DOI: 10.1016/j.bioelechem.2025.109034
Denise Demurtas , Julia Alvarez-Malmagro , Andrés Felipe Quintero-Jaime , Tanushree Mandal , Sébastien Gounel , Thomas M.B. Reichhart , Anna Lielpetere , Alfons K.G. Felice , Christopher Schulz , Roland Ludwig , Marcos Pita , Antonio L. de Lacey , Dónal Leech , Wolfgang Schuhmann , Nicolas Mano , Edmond Magner
{"title":"Enzymatic biofuel cell on flexible nanoporous gold electrodes","authors":"Denise Demurtas , Julia Alvarez-Malmagro , Andrés Felipe Quintero-Jaime , Tanushree Mandal , Sébastien Gounel , Thomas M.B. Reichhart , Anna Lielpetere , Alfons K.G. Felice , Christopher Schulz , Roland Ludwig , Marcos Pita , Antonio L. de Lacey , Dónal Leech , Wolfgang Schuhmann , Nicolas Mano , Edmond Magner","doi":"10.1016/j.bioelechem.2025.109034","DOIUrl":"10.1016/j.bioelechem.2025.109034","url":null,"abstract":"<div><div>A glucose/O<sub>2</sub>-based biofuel cell employing nanoporous gold electrodes (NPG) supported by Kapton® was prepared. The anode was prepared by drop-casting a solution of Crassicarpon hotsonii cellobiose dehydrogenase (ChCDH) and an Os complex-based polymer and the cathodes by covalent immobilization of Magnaporthe oryzae bilirubin oxidase (MoBOD) on 3-mercaptopropionic acid (MPA) self-assembled monolayer (SAM). Both electrodes were coated with poly(2-methacryloyloxyethyl phosphorylcholine-<em>co</em>-glycidyl methacrylate) (MPC) to reduce biofouling. The anode had a J<sub>max</sub> of 172 ± 10 μA cm<sup>−2</sup> and a K<sub>Mapp</sub> of 19 ± 3 mM in phosphate buffer saline, with a linear detection range from 1 to 5 mM and a sensitivity of 9.4 ± 0.3 μA cm<sup>−2</sup> mM<sup>−1</sup>. In artificial plasma, the response was saturated at 3 mM, with J<sub>max</sub> of 6.8 ± 10 μA cm<sup>−2</sup>, K<sub>Mapp</sub> of 1 mM and a linear detection range from 1 to 5 mM. The cathode had a J<sub>max</sub> of 103 μA cm<sup>2</sup> and retained 80 % of its response after 18 h of continuous measurement in phosphate buffered saline, while in artificial plasma, the stability was significantly reduced with a half-life of 1 h under continuous operation. The power outputs in PBS and artificial serum were 4.4 and 1.0 μWcm<sup>−2</sup>, respectively.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109034"},"PeriodicalIF":4.8,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144329989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioelectrochemistryPub Date : 2025-06-16DOI: 10.1016/j.bioelechem.2025.109032
Tong Gao , Bolu Sun , Bei Wang , Xuanxiu Da , Ying Lv , Miao Zhou , Xinlan Wang , Yuqiong Wu , Huajie Li , Haiying He , Jiali Kang , Xiangdong Wang , Chenyu Qin , Lin Yang
{"title":"Self-assembled enzyme biosensors based on xanthine oxidase (XO) and CS@GO for screening natural products for anti-gout active ingredients","authors":"Tong Gao , Bolu Sun , Bei Wang , Xuanxiu Da , Ying Lv , Miao Zhou , Xinlan Wang , Yuqiong Wu , Huajie Li , Haiying He , Jiali Kang , Xiangdong Wang , Chenyu Qin , Lin Yang","doi":"10.1016/j.bioelechem.2025.109032","DOIUrl":"10.1016/j.bioelechem.2025.109032","url":null,"abstract":"<div><div>Gout is a joint disease caused by the deposition of monosodium urate, the incidence of which shows an escalating trend annually while demonstrating a propensity toward younger populations, whereas available pharmacological interventions frequently induce severe adverse effects. In this investigation, a highly sensitive electrochemical enzyme biosensor was constructed based on the pivotal role of xanthine oxidase (XO); the enzymatic activity was enhanced through chitosan - functionalized graphene oxide (CS@GO)-modified electrodes coupled with electrodeposited gold nanoparticles (AuNPs), which, when integrated with their superior electrical conductivity along with exceptional biocompatibility, facilitated the highly efficient screening of anti-gout components within natural products. Following optimization of critical parameters, evaluation of 14 compounds revealed five plant extracts (accounting for 28 % of samples) exhibiting XO inhibition exceeding 83 %, demonstrating superior efficacy compared to allopurinol (83.32 %). This methodology establishes an innovative strategy for rapid identification of highly potent yet minimally toxic anti-gout natural compounds, offering significant reference value regarding natural drug development.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109032"},"PeriodicalIF":4.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioelectrochemistryPub Date : 2025-06-16DOI: 10.1016/j.bioelechem.2025.109030
Peiru Yao , Longfei Xia , Huan Zhang , Hongjian Liao , Shihai Deng , Yaohuan Gao
{"title":"More efficient enriching of electroactive microorganisms from environmental samples by periodic step polarization","authors":"Peiru Yao , Longfei Xia , Huan Zhang , Hongjian Liao , Shihai Deng , Yaohuan Gao","doi":"10.1016/j.bioelechem.2025.109030","DOIUrl":"10.1016/j.bioelechem.2025.109030","url":null,"abstract":"<div><div>Advancement of bioelectrochemical technology depends on efficient screening and enriching of electroactive microorganisms. Yet, conventional methods based on microbial fuel cells or microbial electrolysis cell operation suffer from prolonged operation or false negative results. We propose an anode potential control strategy—periodic step (PS) polarization—for application in screening and enriching electroactive microorganisms from environmental samples. Using fixed potential mode as a control, the performance of PS polarization was examined regarding the start-up time, cultivation duration of three fed-batch cycles, Coulombic efficiency, microbial community composition, and electrochemical properties of biofilms. Electrolysis cells were inoculated by either soil microorganisms or activated sludge. Results demonstrated that the PS polarization significantly shortened the start-up time and duration of the first three cultivation cycles (7–70 days depending on the inoculum) while achieving higher apparent current density and Coulombic efficiency. Besides, the enriched microbial communities under PS polarization showed a higher relative abundance of known electroactive microorganisms. Together with the lower open circuit potential and charge transfer resistance of the biofilm enriched by PS polarization, we concluded that the PS polarization can mitigate the inefficiencies caused by conventional enrichment methods, avoiding trial and error, and can be a novel methodology for enriching electroactive microorganisms.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109030"},"PeriodicalIF":4.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioelectrochemistryPub Date : 2025-06-14DOI: 10.1016/j.bioelechem.2025.109028
Kai Hu , Xin-yue Zhang , Xing Sun , Hang Xu , Wei Chen , Wei Wang
{"title":"Integrated approach to enhance excess sludge degradation in microbial electrolysis cell: Role of ozone pretreatment","authors":"Kai Hu , Xin-yue Zhang , Xing Sun , Hang Xu , Wei Chen , Wei Wang","doi":"10.1016/j.bioelechem.2025.109028","DOIUrl":"10.1016/j.bioelechem.2025.109028","url":null,"abstract":"<div><div>Microbial electrolysis cell (MEC) is an alternative to conventional sludge treatment process with great energy-recovery potential. However, hydrolysis is considered as a rate-limiting step in MEC. In this study, ozone (O<sub>3</sub>) pretreatment was successfully applied to disintegrate sludge matrix and accelerate microbial electrolysis. At 100–250 mg·g<sup>−1</sup> (O<sub>3</sub>/SS), rapid SCOD increment and SS reduction rates were observed with increased O<sub>3</sub> dosage. Afterwards, the mass transfer from gas to liquid was inhibited and oxidation reactions between O<sub>3</sub> and organics occurred, which resulted in a declining disintegration rate. At favorable dosage of 250 mg·g<sup>−1</sup> (O<sub>3</sub>/SS), the degree of disintegration was 17 % and SS reduction reached 44.9 %. A lab-scale MEC experiment was performed by feeding ozonated sludge. Results showed that O<sub>3</sub> pretreatment yielded 8.3-times increment in biogas production rate. In addition, O<sub>3</sub> pretreatment improved the organics removal and bioelectrochemical efficiency during microbial electrolysis, achieving 74.50 % of VSS removal rate and 77.56 % of TCOD removal rate, with gas yield increased by 7.5 times and cathodic hydrogen recovery increased by 7.40 %. The FT-IR spectra indicated negligible difference between influent extracellular biological organic matter (EBOM) and effluent EBOM, which suggested the function of O<sub>3</sub> pretreatment was to accelerate microbial electrolysis reactions due to sludge disintegration. Furthermore, the ozonation pretreatment facilitated the enrichment of exoelectrogens and collaborative bacteria in MEC, collectively enhancing MEC performance. This study provides a theoretical reference for enhanced bioelectrochemical treatment of complex heterogeneous mixture with soluble/insoluble organic matters.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109028"},"PeriodicalIF":4.8,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioelectrochemistryPub Date : 2025-06-14DOI: 10.1016/j.bioelechem.2025.109031
Jiamu Li , Aijia Sun , Han Lai , Chuanhe Li , Huayi Li , Zhengchun Yang , Peng Pan , Jie He , Rui Zhang , Chunhong Wang
{"title":"Ultrasensitive electrochemical aptasensor for Pseudomonas aeruginosa detection using N-doped MWCNTs/AgNPs nanocomposite","authors":"Jiamu Li , Aijia Sun , Han Lai , Chuanhe Li , Huayi Li , Zhengchun Yang , Peng Pan , Jie He , Rui Zhang , Chunhong Wang","doi":"10.1016/j.bioelechem.2025.109031","DOIUrl":"10.1016/j.bioelechem.2025.109031","url":null,"abstract":"<div><div>In this study, an electrochemical sensor for the specific detection of <em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa</em>) was developed using an F23 aptamer-functionalized nitrogen-doped multi-walled carbon nanotubes (N doped-MWCNTs) and silver nanoparticles (AgNPs) composite. Systematic optimization of the Ag/C ratio revealed that a 1:10 composition delivers superior electrochemical performance, owing to synergistic effects between highly dispersed AgNPs and efficient nitrogen doping. Then a biosensor was constructed based on a three-electrode system, featuring a screen-printed electrode (SPE) modified with optimized N-doped MWCNTs/AgNPs-10/F23 aptamer as the working electrode. The structural and compositional characteristics of the sensor materials were systematically characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS). The electrochemical performance was evaluated through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) to assess the conductivity and charge transfer properties of the electrode materials. The sensor exhibited a wide linear detection range from 10<sup>−1</sup> to 10<sup>6</sup> CFU·mL<sup>−1</sup> and the limit of detection is 0.0798 CFU·mL<sup>−1</sup>, demonstrating high specificity and sensitivity for <em>P. aeruginosa</em>. This study demonstrates a novel strategy for developing cost-effective, portable biosensors with exceptional selectivity for bacterial pathogen detection, offering significant potential for real-time environmental monitoring and point-of-care diagnostic applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109031"},"PeriodicalIF":4.8,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioelectrochemistryPub Date : 2025-06-12DOI: 10.1016/j.bioelechem.2025.109033
Sergio J. Ponce-Jahen , Victoria Sibaja-Zepeda , Edgardo I. Valenzuela , Edson B. Estrada-Arriaga , J. Rene Rangel-Mendez , Norma-A. Macías-Ruvalcaba , German Buitron , Bibiana Cercado , Francisco J. Cervantes
{"title":"Conversion of ammonium to nitrous oxide in a microbial electrochemical cell","authors":"Sergio J. Ponce-Jahen , Victoria Sibaja-Zepeda , Edgardo I. Valenzuela , Edson B. Estrada-Arriaga , J. Rene Rangel-Mendez , Norma-A. Macías-Ruvalcaba , German Buitron , Bibiana Cercado , Francisco J. Cervantes","doi":"10.1016/j.bioelechem.2025.109033","DOIUrl":"10.1016/j.bioelechem.2025.109033","url":null,"abstract":"<div><div>Ammonium (NH<sub>4</sub><sup>+</sup>) is a major pollutant in wastewater, and its removal using conventional methods is often energy-intensive and costly. This proof-of-concept study investigates microbial electrochemical cells (MECs) as a sustainable alternative for NH<sub>4</sub><sup>+</sup> removal and nitrous oxide (N<sub>2</sub>O) recovery. The study focuses on partial nitrification and partial denitrification processes occurring in MECs, emphasizing the electrochemical and microbial mechanisms responsible for NH<sub>4</sub><sup>+</sup> conversion. Using <sup>15</sup>NH<sub>4</sub><sup>+</sup> tracer analysis, the study demonstrates a 76 % conversion of NH<sub>4</sub><sup>+</sup> to N<sub>2</sub>O, driven by biofilms containing denitrifying microorganisms, such as <em>Simpliscispira</em> and <em>Thiobacillus</em>, and as well as planktonic species like <em>Phycisphaerae, Anaerobacterium</em>, <em>Rikenellaceae</em>, and <em>Synergistaceae</em>. MECs exhibited high energy efficiency, requiring only 1.64 kWh/kg of N-NH<sub>4</sub><sup>+</sup> removed, a value significantly lower than conventional methods. These findings underscore the potential of MECs as a promising technology for sustainable nitrogen management, demonstrating both nitrogen removal and N<sub>2</sub>O valorization for industrial application. Further optimization of microbial communities and operational parameters is needed to maximize process efficiency and scalability.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109033"},"PeriodicalIF":4.8,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioelectrochemistryPub Date : 2025-06-11DOI: 10.1016/j.bioelechem.2025.109029
Yanjun Wang , Zhenyuan Yang , Shaojie Xia , Junjie Huang , Yonghong Wang , Ge Ning
{"title":"Electrochemical-colorimetric dual-mode detection of zearalenone based on restriction endonuclease-driven DNA walker system","authors":"Yanjun Wang , Zhenyuan Yang , Shaojie Xia , Junjie Huang , Yonghong Wang , Ge Ning","doi":"10.1016/j.bioelechem.2025.109029","DOIUrl":"10.1016/j.bioelechem.2025.109029","url":null,"abstract":"<div><div>Zearalenone (ZEN) is a toxic metabolite produced mainly by strains of <em>Fusarium</em> spp. and is characterized by high toxicity and easy residue. Prolonged exposure to ZEN-contaminated grains can produce a range of toxic effects in the body and affect human health. In this study, an electrochemical and colorimetric dual-mode biosensor for ZEN detection was developed based on streptavidin-modified magnetic beads (SA-MBs) and the DNA walker. In this strategy, Apt/Walker could be used to specifically recognize ZEN and trigger two endonuclease-driven walker reactions, which resulted in the disruption and cleavage of orbital chains on SA-MBs and on the electrode surface. This ultimately led to dynamic changes in the colorimetric signal of the solution and the electrochemical signal on the electrode surface. The integration of target recognition with dual signal amplification and the reliability of dual mode detection were some of the advantages of the designed sensor. Under optimal conditions, the detection limits for both modes were 3.44 × 10<sup>−10</sup> mol/L (colorimetry) and 3.39 × 10<sup>−9</sup> mol/L (electrochemistry), respectively. At the same time, this dual-mode sensor had good specificity and recovery. It addressed the limitations of traditional detection methods and has broad potential in areas such as food safety.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109029"},"PeriodicalIF":4.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioelectrochemistryPub Date : 2025-06-04DOI: 10.1016/j.bioelechem.2025.109014
Daniela Torruella-Salas , Carlos Manchon , Andrés de Deus , Pamela Torres-Salas , Laura Gómez-Espina , Aurora Mañas-Fernández , Antonio Berná , Abraham Esteve-Núñez
{"title":"Microbial electrochemical biosensor for real-time in situ hydrocarbon detection in groundwater","authors":"Daniela Torruella-Salas , Carlos Manchon , Andrés de Deus , Pamela Torres-Salas , Laura Gómez-Espina , Aurora Mañas-Fernández , Antonio Berná , Abraham Esteve-Núñez","doi":"10.1016/j.bioelechem.2025.109014","DOIUrl":"10.1016/j.bioelechem.2025.109014","url":null,"abstract":"<div><div>Anthropogenic contamination with petroleum hydrocarbons is a serious environmental problem. Especially dramatic is the case of environments limited in electron acceptors because, natural attenuation is not sufficient to cope with contamination that remains persistent. Groundwater pollution by oil spills has attracted an ever-growing interest in a water scarcity scenario due to global warming. The surveillance of risk sites through monitoring tools is the most suitable strategy for prevention. In this work we have investigated the detection of contaminants derived from oil industry, BTEX and ETBE, in groundwater by means of a microbial electrochemical sensor. For this purpose, we use a biosensor (polarized at 0.6 V) immersed in artificial groundwater: i) at microcosm scale and ii) at mesocosm scale. Detection of BTEX was tested separately in biosensors at microcosm scale, showing a clear response in electrical current after exposure to contaminants. The response observed in presence of BTEX and ETBE was significant and fast (<2 h) by this biosensor. Additionally, microbial community analysis was performed on the anodic biofilm after BTEX exposure. The results show two main groups of microorganisms: electroactive and hydrocarbon-degrading bacteria. This suggests that the microbial community could play an important role in the biosensor performance.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109014"},"PeriodicalIF":4.8,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}