Bioelectrochemistry最新文献

Enhanced corrosion resistance and biofilm inhibition of TC4 with slight Cu addition against marine Pseudomonas aeruginosa. 添加少量铜的 TC4 可增强对海洋铜绿假单胞菌的耐腐蚀性和生物膜抑制作用。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-19 DOI: 10.1016/j.bioelechem.2024.108852

Shengchao Yao, Yulin Chen, Xin Zhang, Zhizhong Dong

{"title":"Enhanced corrosion resistance and biofilm inhibition of TC4 with slight Cu addition against marine Pseudomonas aeruginosa.","authors":"Shengchao Yao, Yulin Chen, Xin Zhang, Zhizhong Dong","doi":"10.1016/j.bioelechem.2024.108852","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2024.108852","url":null,"abstract":"<p><p>Ti-6Al-4V (TC4) alloy is widely utilized as the structural material in marine industries owing to its low density, high specific strength, and favorable corrosion resistance. However, as biofouling drastically alters, some reported the major deleterious effect of bacteria has imposed a challenge to improve microbiologically influenced corrosion (MIC) resistance. A further opportunity for solving this problem is Cu micro-alloying, which was inspired by adding Cu for biomedical applications. Herein, a Ti-6Al-4V alloy with slight Cu addition (TC4-Cu) was exposed to 2216E media inoculated with Pseudomonas aeruginosa (P. A.), and then investigated compared to TC4. TC4-Cu exhibits lower corrosion current, more denser passive film, and lower weight loss with weaker pitting (a maximum pitting depth of 0.2 μm), compared to TC4 with a maximum pitting crater depth of 9.6 μm. Those demonstrated that the presence of Cu significantly improved the MIC resistance, and inhibited the proliferation of P. A., leading to a good antimicrobial efficacy against marine P. A. Moreover, besides the well-known bactericidal role, Cu ions were transferred to form Cu<sub>2</sub>O and CuO, constituting protective corrosion products, and thus improving the anti-microbial properties of TC4-Cu.</p>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"108852"},"PeriodicalIF":4.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Molecular monolayers on silicon as substrates for biosensors" [Bioelectrochem. 80(1) (2010) 17-25]. 硅上的分子单层作为生物传感器的基底"[Bioelectrochem.

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-17 DOI: 10.1016/j.bioelechem.2024.108849

L Touahir, P Allongue, D Aureau, R Boukherroub, J-N Chazalviel, E Galopin, A C Gouget-Laemmel, C Henry de Villeneuve, A Moraillon, J Niedziółka-Jönsson, F Ozanam, J Salvador Andresa, S Sam, I Solomon, S Szunerits

引用次数: 0

Label-free electrochemical biosensor with magnetic self-assembly constructed via PNA-DNA hybridization process on α-Fe2O3/Fe3O4 nanosheets for APOE ε4 genes ultrasensitive detection 通过 PNA-DNA 杂交过程在 α-Fe2O3/Fe3O4 纳米片上构建的磁性自组装无标记电化学生物传感器，用于 APOE ε4 基因的超灵敏检测

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-15 DOI: 10.1016/j.bioelechem.2024.108847

Zhihao Xu , Zhixiang Lv , Huijiao Yang , Jiashuo Zhang , Zijie Sun , Dawei He , Ruijiang Liu

{"title":"Label-free electrochemical biosensor with magnetic self-assembly constructed via PNA-DNA hybridization process on α-Fe2O3/Fe3O4 nanosheets for APOE ε4 genes ultrasensitive detection","authors":"Zhihao Xu , Zhixiang Lv , Huijiao Yang , Jiashuo Zhang , Zijie Sun , Dawei He , Ruijiang Liu","doi":"10.1016/j.bioelechem.2024.108847","DOIUrl":"10.1016/j.bioelechem.2024.108847","url":null,"abstract":"<div><div>A label-free electrochemical DNA detection strategy based on self-assembled α-Fe<sub>2</sub>O<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub> nanosheets with PNA-DNA hybridization process was developed for ultrasensitive detection of APOE ε4 gene, one of the most robust genetic risks for Alzheimer’s Disease (AD). In this work, magnetic α-Fe<sub>2</sub>O<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub> heterogeneous nanosheets were prepared by hydrothermal-calcined reduction method and loaded with Au nanoparticles (AuNPs) on their surfaces. The magnetic α-Fe<sub>2</sub>O<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub>@Au nanocomposites significantly enhanced the electrochemical response as a signal amplification matrix and were able to bind to the magnetic glassy carbon electrode (MGCE) surface by magnetic self-assembly. Moreover, owing to the high specificity and stable binding capacity of PNA with respect to the target DNA, the biosensor not only enabled accurate (the limit of detection was estimated to be 0.147 pM) and rapid detection of the APOE ε4 gene, but also exhibited excellent specificity, stability and regeneration capability. Additional, the satisfactory recoveries were also obtained in real samples of human serum, ranging from 92.83 % to 106.22 % with relative standard deviation (RSD) between 0.25 % and 1.85 %. The results possessed important reference value for exploring the application of DNA biosensor technology in the diagnosis of APOE gene mutation.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108847"},"PeriodicalIF":4.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142656712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time-resolved electromechanical and conductive behavior of nanostructured bilayers tethered to the surface of the electrode with incorporated channel proteins and peptides. 用通道蛋白和肽将纳米结构双分子层系在电极表面的时间分辨机电和导电行为。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-15 DOI: 10.1016/j.bioelechem.2024.108848

Aleksandra Stefanowska, Michał Czapczyński, Piotr Koprowski, Adam Szewczyk, Paweł Krysiński

引用次数: 0

Sensitive detection of K-ras gene by a dual-mode “on-off-on” sensor based on bipyridine ruthenium-MOF and bis-enzymatic cleavage technology 基于联吡啶钌-MOF 和双酶裂解技术的 "开-关-开 "双模式传感器对 K-ras 基因的灵敏检测。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-05 DOI: 10.1016/j.bioelechem.2024.108845

Haotian Xie , Zhaojiang Yin , Guobin Wei , Binghui Li , Hanfeng Cui , Hao Fan , Jing Zhang

引用次数: 0

A novel label-free impedance biosensor for KRAS G12C mutations detection based on PET-RAFT and ROP synergistic signal amplification 基于 PET-RAFT 和 ROP 协同信号放大技术的新型无标记阻抗生物传感器，用于检测 KRAS G12C 突变。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-02 DOI: 10.1016/j.bioelechem.2024.108844

Yaping Zhang , Haiyan Wei , Liang Guo , Wei Gao , Di Cheng , Yanju Liu

{"title":"A novel label-free impedance biosensor for KRAS G12C mutations detection based on PET-RAFT and ROP synergistic signal amplification","authors":"Yaping Zhang , Haiyan Wei , Liang Guo , Wei Gao , Di Cheng , Yanju Liu","doi":"10.1016/j.bioelechem.2024.108844","DOIUrl":"10.1016/j.bioelechem.2024.108844","url":null,"abstract":"<div><div>The KRAS G12C mutations, as crucial biomarkers, are closely associated with non-small cell lung cancer. Here, a novel label-free electrochemical biosensor with synergistic signal amplification of photocell energy transfer-reversible addition fragmentation chain transfer (PET-RAFT) and ring-opening polymerization (ROP) was developed for the first time for sensitive detection of KRAS G12C mutations. Specifically, hairpin DNA (hDNA), which act as biomolecular probe, was self-assembled on Au electrode surface by Au-S bond. 4-cyano-4-[(dodecylsulfanylthiocarbonyl) sulfanyl] pentanoic acid (CDTPA), the chain transfer agent of PET-RAFT reaction, was then attached to hDNA via amide bond. After that, the target DNA (tDNA) was captured on the electrode surface by complementary base pairing with hDNA. Subsequently, large numbers of electro-active monomers N-acryloxysuccinimide (NAS) were successfully grafted to the electrode surface via PET-RAFT reaction, which provided plenty of junction sites for doxorubicin-polycaprolactone (Dox-PCL) synthesized by ROP. Finally, the Dox-PCL was connected to the electrode surface by ester bond, significantly amplifying the electrochemical signal. Under optimized conditions, the biosensor has a wide linear detection range of 0.1 pM to 1 μM, with a detection limit of 86.9 fM. Attribute to its high sensitivity, specificity, reproducibility and stability, this biosensor possesses considerable potential in early diagnosis of disease and biomedical research.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108844"},"PeriodicalIF":4.8,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Analysis of electromagnetic response of cells and lipid membranes using a model-free method" [Bioelectrochemistry 152 (2023) 108444]. 对 "利用无模型方法分析细胞和脂膜的电磁响应"[生物电化学 152 (2023) 108444] 的更正。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-02 DOI: 10.1016/j.bioelechem.2024.108841

Yingxian Lu, Xiaping Tang, Yanyu Zhao, Tianyu Jiang, Jiayao Zhou, Xiaofei Wang, Bing Huang, Lingyu Liu, Hu Deng, Yujing Huang, Yigong Shi

引用次数: 0

Advanced laser-induced graphene-based electrochemical immunosensor for the detection of C-reactive protein 基于石墨烯的先进激光诱导电化学免疫传感器，用于检测 C 反应蛋白。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-10-28 DOI: 10.1016/j.bioelechem.2024.108842

Sri Ramulu Torati , Gymama Slaughter

{"title":"Advanced laser-induced graphene-based electrochemical immunosensor for the detection of C-reactive protein","authors":"Sri Ramulu Torati , Gymama Slaughter","doi":"10.1016/j.bioelechem.2024.108842","DOIUrl":"10.1016/j.bioelechem.2024.108842","url":null,"abstract":"<div><div>C-reactive protein (CRP) is a critical biomarker for detecting inflammation and forecasting cardiovascular disease. We present an advanced electrochemical immunosensor utilizing laser-induced graphene (LIG)/MXene-gold nanoparticles (Mx-AuNPs) electrode for CRP detection. The Mx-AuNPs nanocomposite, synthesized via in-situ reduction of HAuCl<sub>4</sub> by MXene, leverages MXene’s reducing properties for effective nanoparticle deposition, confirmed through scanning electron microscopy. This electrode demonstrates superior electrochemical performance due to enhanced surface area and synergy between LIG and Mx-AuNPs, improving overall electrode conductivity. The A-CRP antibody, immobilized via a cysteamine linker, enables CRP detection. The immunosensor achieves excellent detection across 10 pg mL<sup>−1</sup> to 10 µg mL<sup>−1</sup> CRP, with a low detection limit of 1.45 pg mL<sup>−1</sup>, and shows high selectivity for CRP. This LIG/Mx-AuNPs-based immunosensor is promising for sensitive CRP detection, aiding early cardiovascular disease diagnosis and improving patient outcomes.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108842"},"PeriodicalIF":4.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the cell quantity of CRISPR/Cas9 gene editing by continuous microfluidic electroporation chip 利用连续微流控电穿孔芯片扩大 CRISPR/Cas9 基因编辑的细胞数量。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-10-28 DOI: 10.1016/j.bioelechem.2024.108840

Zixi Li , Xinyue Su , Yihong Lin, Yu Zhang, Anlan Zhang, Xin Wu, Xi Jiyu, Qin Li, Zewen Wei

{"title":"Expanding the cell quantity of CRISPR/Cas9 gene editing by continuous microfluidic electroporation chip","authors":"Zixi Li , Xinyue Su , Yihong Lin, Yu Zhang, Anlan Zhang, Xin Wu, Xi Jiyu, Qin Li, Zewen Wei","doi":"10.1016/j.bioelechem.2024.108840","DOIUrl":"10.1016/j.bioelechem.2024.108840","url":null,"abstract":"<div><div>CRISPR/Cas9-mediated gene editing offers promising and safe therapeutic options for a wide range of diseases. The technical difficulty of efficiently acquiring large quantities of gene-edited therapeutic cells in a short time period is now preventing the widespread clinical application of CRISPR/Cas9-mediated gene editing. Herein, a Large Volume Continuous Electroporation Chip (LaViE-Chip) has been developed to address the challenge of acquiring sufficient quantities of genetically edited cells for CRISPR/Cas9 gene editing. By connecting multiple relatively narrow microfluidic channels in parallel, a satisfactory balance between cell flow volume and electric field uniformity was achieved with two simple off-chip electrodes, which also isolated harmful effects around electrodes from target cells. Meanwhile, by carefully designing the curvature of the microfluidic channel, hydrodynamic controlled rotation of target cells has been realized to improve the transfection efficiency and cell viability. With these improvements, the LaViE-Chip realized 71.06 % electrotransfection efficiency, 84.3 % cell viability, and 10<sup>7</sup> cell/min cell processing speed. Moreover, the first successful incessant CRISPR gene editing by electroporation has been demonstrated, laying the technical foundation of therapeutic CRISPR gene editing.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108840"},"PeriodicalIF":4.8,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitive electrochemiluminescence immunosensor for CEA detection based on the ECL-RET between zinc-based metal–organic frameworks and ZiF-8@PDA 一种基于锌基金属有机框架和 ZiF-8@PDA 之间的 ECL-RET 的灵敏 CEA 检测电化学发光免疫传感器。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-10-26 DOI: 10.1016/j.bioelechem.2024.108843

Yige Li, Yingying Cheng, Haoyi Ren, Tiantian Ji, Zhengyi Zhao, Hongling Li, Chenglin Hong

{"title":"A sensitive electrochemiluminescence immunosensor for CEA detection based on the ECL-RET between zinc-based metal–organic frameworks and ZiF-8@PDA","authors":"Yige Li, Yingying Cheng, Haoyi Ren, Tiantian Ji, Zhengyi Zhao, Hongling Li, Chenglin Hong","doi":"10.1016/j.bioelechem.2024.108843","DOIUrl":"10.1016/j.bioelechem.2024.108843","url":null,"abstract":"<div><div>In this study, we developed a new system that using zinc-based metal–organic frameworks NH<sub>2</sub>-Zn-PTC as the donor and ZiF-8@PDA as the acceptor to achieve highly sensitive detection of carcinoembryonic antigen (CEA), using the fundamentals of electrochemiluminescence resonance energy transfer (ECL-RET). Firstly, the aggregation-induced quenching effect (ACQ) was eliminated by the coordination of PTC in MOF and the ECL signal was improved. Secondly, the ECL signal was further amplified by using Au NPs and amino groups as co-reaction promoters to generate more SO<sub>4</sub><sup>.−</sup>. In addition, the introduction of ZiF-8@PDA as an acceptor and NH<sub>2</sub>-Zn-PTC as a donor took advantage of the feature of partial overlap of the UV–vis absorption spectrum and ECL emission spectra between the two, thereby effectively initiating the ECL-RET behavior, which improved the detection sensitivity of the sensor. The prepared immunosensor showed good linearity in the concentration range of 10<sup>−4</sup> to 80 ng/mL with a detection limit of 18.20 fg/mL. This makes it promising for clinical testing of tumor markers.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108843"},"PeriodicalIF":4.8,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0