Bioelectrochemistry最新文献

Dynamic residue ionization in a bacterial porin: Impact of ionic strength and membrane potential. 细菌孔蛋白中的动态残基电离：离子强度和膜电位的影响。

IF 4.9 2区化学

Bioelectrochemistry Pub Date : 2027-02-01 Epub Date: 2026-08-26 DOI: 10.1016/j.bioelechem.2026.109440

Ernesto Tavares-Neto, Marcel Aguilella-Arzo, Vicente M Aguilella

{"title":"Dynamic residue ionization in a bacterial porin: Impact of ionic strength and membrane potential.","authors":"Ernesto Tavares-Neto, Marcel Aguilella-Arzo, Vicente M Aguilella","doi":"10.1016/j.bioelechem.2026.109440","DOIUrl":"10.1016/j.bioelechem.2026.109440","url":null,"abstract":"<p><p>The protonation states of key residues play a critical role in regulating the function of membrane proteins. Also, protein channel characterization involves testing the effect of pH, membrane charge, ionic concentration, and applied potential as modulators of channel conductance, selectivity and gating. Using GROMACS Constant-pH Molecular Dynamics, here we investigate how ionic strength, externally applied voltage and structural conformation changes modulate the protonation state of the acidic residues of the OmpF channel. pKa shifts in response to increased KCl concentrations do not follow a simple pattern. This contrasts with earlier claims on the effect of ionic strength on the pKa of selected OmpF residues. Additionally, we find that applying an external voltage yields position-dependent small pKa shifts of opposite sign on the periplasmic and the extracellular side of the protein. This charge regulation coming from the external electric field adds to the well-known structural charge asymmetry of this channel. While pKa shifts involve minimal protein backbone conformation changes, there are some significant side chains rotations or displacements. Overall, these findings provide new insights into how different factors influence the protonation dynamics of OmpF. The results may have useful implications for other channels regulated by long-range electrostatic interactions.</p>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"173 ","pages":"109440"},"PeriodicalIF":4.9,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148856864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Super-antifouling electrochemical biosensor based on a biomimetic gemini zwitterionic interface for sensitive detection of therapeutic antibodies. 基于仿生双两性离子界面的超防污电化学生物传感器，用于治疗性抗体的灵敏检测。

IF 4.9 2区化学

Bioelectrochemistry Pub Date : 2027-02-01 Epub Date: 2026-08-24 DOI: 10.1016/j.bioelechem.2026.109437

Zheng Zhao, Wanqing Yu, Haidong Li, MingFang Hai, Zhiwei Chen, Hai Han, Jia-Huan Qu, Qiqin Wang, Zhengjin Jiang

{"title":"Super-antifouling electrochemical biosensor based on a biomimetic gemini zwitterionic interface for sensitive detection of therapeutic antibodies.","authors":"Zheng Zhao, Wanqing Yu, Haidong Li, MingFang Hai, Zhiwei Chen, Hai Han, Jia-Huan Qu, Qiqin Wang, Zhengjin Jiang","doi":"10.1016/j.bioelechem.2026.109437","DOIUrl":"10.1016/j.bioelechem.2026.109437","url":null,"abstract":"<p><p>Biofouling, arising from the nonspecific adsorption of proteins, cells, and other biomolecules, remains a major challenge that compromises the stability and reliability of diagnostic and therapeutic platforms. To address this issue, a super-antifouling electrochemical aptasensor was developed by integrating a biomimetic \"gemini\" zwitterionic monomer (BSMMP) with polydopamine (PDA). The covalent assembly of BSMMP and PDA forms a multi-site anchoring layer that enables the stable functionalization of the affinity aptamer GC20. Owing to its dense hydration shell, the PDA-BSMMP hybrid interface acts as a physical barrier against nonspecific adsorption, maintaining electron-transfer stability in undiluted human serum. Differential pulse voltammetry confirmed that this interface markedly reduced biofouling-induced signal loss, limiting attenuation to less than 15%, nearly fourfold lower than that of the unmodified surface. The platform achieved a low limit of detection of 0.97 ng/mL, a broad linear range from 1 ng/mL to 100 μg/mL, and high selectivity for trastuzumab in a label-free format without secondary antibodies or additional signal amplification. Finally, this platform successfully quantified trastuzumab in serum from breast cancer patients, with results consistent with commercial ELISA kits. Overall, this super-antifouling aptasensor offers great potential for therapeutic drug monitoring, advancing zwitterionic interface-based biosensing strategies for point-of-care diagnostics.</p>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"173 ","pages":"109437"},"PeriodicalIF":4.9,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Surface activation and functionalization with aryl-diazonium chemistry enable biomolecules grafting on 3D-printed carbon-based electrodes" [Bioelectrochemistry 170 (2026) 109254]. “芳基重氮化学的表面活化和功能化使生物分子能够在3d打印的碳基电极上接枝”[生物电化学170(2026)109254]的勘误表。

IF 4.9 2区化学

Bioelectrochemistry Pub Date : 2027-02-01 Epub Date: 2026-08-01 DOI: 10.1016/j.bioelechem.2026.109411

Mathilde Manceau, Carole Farre, Laurène Tétard, François Bessueille, Marie Martin, Jasmina Vidic, Yanxia Hou, Carole Chaix, Florence Lagarde

引用次数: 0

Electric stimulation coupled with activated carbon enhances lactic acid production from food waste. 电刺激加上活性炭可以提高食物垃圾中乳酸的产量。

IF 4.9 2区化学

Bioelectrochemistry Pub Date : 2027-02-01 Epub Date: 2026-08-13 DOI: 10.1016/j.bioelechem.2026.109429

Nuohan Wang, Jianguo Liu, Juan Wang, Xiaona Wang, Pan Zhao, Qunhui Wang

{"title":"Electric stimulation coupled with activated carbon enhances lactic acid production from food waste.","authors":"Nuohan Wang, Jianguo Liu, Juan Wang, Xiaona Wang, Pan Zhao, Qunhui Wang","doi":"10.1016/j.bioelechem.2026.109429","DOIUrl":"10.1016/j.bioelechem.2026.109429","url":null,"abstract":"<p><p>High-value recycling of food waste (FW) can be accomplished through the production of lactic acid (LA). In this work, LA was produced via electro-fermentation (EF) on FW as a single substrate in non-sterile, non-inoculated environment. Electric stimulation (ES) coupled with granular activated carbon (GAC) was used to increase LA production. The findings demonstrated that the addition of 10 g/L of GAC to the system at a voltage of -1 V for 144 h of fermentation resulted in an increase in the LA concentration and maximum production rate of 33.6% and 12.2%, respectively, when compared to the Blank group. The experimental group coupled with ES and GAC also exhibited the highest intracellular NADH/NAD<sup>+</sup> ratio, a notable increase in the abundance of Actinobacteriota and Proteobacteria, and prominent redox peaks. In terms of the synergistic regulatory effect of key enzymes, the activity of α -glucosidase was enhanced. Besides, the abundance of related enzymes such as lactate dehydrogenase increased, significantly strengthening the glycolytic pathway for LA production and inhibiting the heterolactic fermentation process. The results of this study provide an effective way to recycle FW and provide theoretical support for the analysis of the synergistic mechanism of electron transfer and metabolic regulation in the EF system.</p>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"173 ","pages":"109429"},"PeriodicalIF":4.9,"publicationDate":"2027-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Development of sandwich electrochemiluminescence immunosensor for COVID-19 diagnosis by SARS-CoV-2 spike protein detection based on Au@BSA-luminol nanocomposites" [Bioelectrochemistry 147 (2022) 108161]. “基于Au@BSA-luminol纳米复合材料的SARS-CoV-2刺突蛋白检测诊断COVID-19的三明治电化学发光免疫传感器的研制”[生物电化学147(2022)108161]。

IF 4.9 2区化学

Bioelectrochemistry Pub Date : 2027-02-01 Epub Date: 2026-08-29 DOI: 10.1016/j.bioelechem.2026.109435

Morteza Hosseini, Ebtesam Sobhanie, Foad Salehnia, Guobao Xu, Hodjattallah Rabbani, Mahsa Naghavi Sheikholeslami, Ali Firoozbakhtian, Niloufar Sadeghi, Mohammad Hossein Farajollah, Mohammad Reza Ganjali, Houman Vosough

引用次数: 0

Microalgae cells with extracellular gold nanoparticles for enhanced photobioelectrochemical activity 微藻细胞与细胞外金纳米粒子增强光生物电化学活性

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-10-01 Epub Date: 2026-03-04 DOI: 10.1016/j.bioelechem.2026.109264

Caio C.G. Silva , Alessandro Cacia , Hernán D. Rojas-Mantilla , Maria V.B. Zanoni , Saulo S. Garrido , Felipe Conzuelo

{"title":"Microalgae cells with extracellular gold nanoparticles for enhanced photobioelectrochemical activity","authors":"Caio C.G. Silva , Alessandro Cacia , Hernán D. Rojas-Mantilla , Maria V.B. Zanoni , Saulo S. Garrido , Felipe Conzuelo","doi":"10.1016/j.bioelechem.2026.109264","DOIUrl":"10.1016/j.bioelechem.2026.109264","url":null,"abstract":"<div><div>Photobioelectrochemical systems (PBEs) harness the machinery of photosynthetic microorganisms to convert solar energy into electricity. However, the inefficient electron transfer at the cell-electrode interface remains as the key performance limitation. Herein, we report a plasmonic biohybrid strategy to enhance extracellular electron transfer in <em>Chlorella</em>-based photobioelectrodes by integrating gold nanoparticles (AuNPs) localized on the microalgae membrane. Two approaches are investigated, consisting of physical mixing of isolated cells with AuNPs and cultivating microalgae in a growth medium supplemented with AuNPs, with the second approach allowing to obtain about significantly higher photocurrent responses. Optimized bioelectrodes yield photocurrent densities of up to 132 μA cm<sup>−2</sup>, 74% higher than bioelectrodes fabricated using unmodified cells. Spectral response analysis reveals a strong resonance at 525 nm, consistent with the plasmonic properties of AuNPs. Importantly, pigment content, growth kinetics, and membrane integrity are preserved, confirming the biocompatibility of the modification. This work presents a facile and effective route to engineer photosynthetic bioelectrodes using nanomaterials, advancing the design of high-performance PBEs for solar-to-electricity conversion.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"171 ","pages":"Article 109264"},"PeriodicalIF":4.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anthraquinone disulfonate as a stable redox mediator for efficient air-cathodes at neutral pH in dual-chamber microbial fuel cells 二磺酸蒽醌作为双室微生物燃料电池中性pH下高效空气阴极的氧化还原介质

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-10-01 Epub Date: 2026-03-09 DOI: 10.1016/j.bioelechem.2026.109279

Antoine Vautier, James A. Behan, Charlotte Bodin, Florence Geneste, Frédéric Barrière

{"title":"Anthraquinone disulfonate as a stable redox mediator for efficient air-cathodes at neutral pH in dual-chamber microbial fuel cells","authors":"Antoine Vautier, James A. Behan, Charlotte Bodin, Florence Geneste, Frédéric Barrière","doi":"10.1016/j.bioelechem.2026.109279","DOIUrl":"10.1016/j.bioelechem.2026.109279","url":null,"abstract":"<div><div>Microbial Fuel Cells (MFCs) are commonly developed as organic-matter oxidizing bioanodes with abiotic air cathodes. However, O<sub>2</sub> reduction requires active aeration and/or the use of expensive catalysts using noble metals. In this study, 2,7-anthraquinone disulfonate (2,7-AQDS), an organic redox mediator commonly used in aqueous redox flow battery systems (AORFBs), served as a redox-stable intermediate for oxygen reduction. Dual-chamber MFC pilots were developed with 2,7-AQDS in the catholyte under both anoxic and aerobic conditions and compared to pilots with ferricyanide catholytes. In both conditions, cyclic voltammetry studies confirmed similar and efficient electroactivity despite the proximity AQDS formal redox potential to that of acetate oxidation. Mediated air-cathodes achieved open-circuit voltage (OCV) of 510 mV and current densities of 140 μA/cm<sup>2</sup>, nearly double those of air-only cathodes (72 μA/cm<sup>2</sup>), while delivering a 33% higher power density (12 mW/m<sup>2</sup> vs. 8 mW/m<sup>2</sup>). Passive catholyte aeration enabled continuous reoxidation of reduced 2,7-AQDS at 8.8 × 10<sup>−8</sup> mol/s, exceeding the AQDS reduction rate by the bioanode (2.5 × 10<sup>−10</sup> mol/s), thus ensuring effective self-regeneration and stable AQDS concentration. These results demonstrate that AQDS coupled with passive oxygen supply sustains biofilm activity with enhances current and power, and allow long-term / low-maintenance MFC operation and organic-matter oxidation.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"171 ","pages":"Article 109279"},"PeriodicalIF":4.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing fast scan cyclic voltammetry at carbon fiber microelectrodes to quantify short chain fatty acids in situ 碳纤维微电极快速扫描循环伏安法原位定量短链脂肪酸。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-10-01 Epub Date: 2026-03-04 DOI: 10.1016/j.bioelechem.2026.109266

Terdha Narla , Uma Nudurupati, Yangguang Ou

{"title":"Developing fast scan cyclic voltammetry at carbon fiber microelectrodes to quantify short chain fatty acids in situ","authors":"Terdha Narla , Uma Nudurupati, Yangguang Ou","doi":"10.1016/j.bioelechem.2026.109266","DOIUrl":"10.1016/j.bioelechem.2026.109266","url":null,"abstract":"<div><div>Acetic, propionic, and butyric acids are short chain fatty acids (SCFAs) and the most abundant metabolites produced by gut bacteria. To uncover mechanistic insights of their function throughout the body, it is critical to measure rapid SCFA fluxes in sites of action such as brain, muscle, and skin. Current approaches have focused on fecal and plasma measurements, where SCFA levels are low and not representative of tissue-specific concentrations or fluxes. Thus, a rapid, <em>in situ</em> methodology is needed. Fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes (CFMs) has the spatiotemporal resolution to fill this need. However, the electrochemical response of CFMs to SCFAs have not been explored until now. By coupling FSCV at CFMs with flow injection analysis of each SCFA, we demonstrate there are distinct peaks in the cyclic voltammograms for each fatty acid. Some of these peaks display faradaic behavior in scan rate, holding potential, and switching potential experiments. Interestingly, we identify several peaks that are concentration-sensitive and therefore are promising quantifiable markers of SCFA dynamics and fluxes. This work lays the foundation in understanding the response of CFMs to fatty acids and demonstrate the utility of FSCV at CFMs for the <em>in situ</em> quantitation of SCFAs.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"171 ","pages":"Article 109266"},"PeriodicalIF":4.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147371938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotip acetylcholine biosensor reveals cholinergic differentiated SH-SY5Y cells release partial vesicle content during exocytosis 纳米尖端乙酰胆碱生物传感器显示胆碱能分化的SH-SY5Y细胞在胞吐过程中释放部分囊泡内容物

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-10-01 Epub Date: 2026-02-26 DOI: 10.1016/j.bioelechem.2026.109260

Yuanmo Wang , Ajay Pradhan , Pankaj Gupta , Jörg Hanrieder , Henrik Zetterberg , Ann-Sofie Cans

{"title":"Nanotip acetylcholine biosensor reveals cholinergic differentiated SH-SY5Y cells release partial vesicle content during exocytosis","authors":"Yuanmo Wang , Ajay Pradhan , Pankaj Gupta , Jörg Hanrieder , Henrik Zetterberg , Ann-Sofie Cans","doi":"10.1016/j.bioelechem.2026.109260","DOIUrl":"10.1016/j.bioelechem.2026.109260","url":null,"abstract":"<div><div>Acetylcholine (ACh) is a central neurotransmitter in cognitive function, motor control, and synaptic modulation, yet its electrochemical inactivity and the rapid kinetics of exocytosis have hindered real-time quantal measurements. Micrometer-scale enzymatic ACh biosensors previously enabled sub-millisecond extracellular recordings but were too large for synaptic positioning and intracellular recordings. Here we present a short, ultrafast and low-noise amperometric ACh biosensor based on a needle-shaped carbon fiber nanotip electrode functionalized with gold nanoparticles and enzymes. The miniaturized geometry allows precise placement at neurite release sites and minimally invasive insertion into the cell cytoplasm, enabling high-temporal resolution monitoring of presynaptic exocytosis together with quantification of intracellular ACh vesicle content. We applied this platform to differentiated human cholinergic SH-SY5Y neuroblastoma cells, an established yet underutilized cell model for cholinergic signaling. The nanotip sensor successfully captured amperometric spikes from both intracellular vesicle burst events and presynaptic ACh release. Intracellular events released a larger amount of ACh than presynaptic exocytosis events, indicating a predominance of partial exocytosis mode in these cells. These results demonstrate the nanotip ACh biosensor as a unique tool for probing fusion pore dynamics at subcellular resolution and for providing quantitative insight into the quantal nature of cholinergic signaling in human neuronal models.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"171 ","pages":"Article 109260"},"PeriodicalIF":4.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemistry of redox enzymes: from functional enzyme immobilization to enzymatic bio-electrochemical devices (tutorial) 氧化还原酶的电化学：从功能酶固定化到酶生物电化学装置（教程）

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-10-01 Epub Date: 2026-03-04 DOI: 10.1016/j.bioelechem.2026.109265

A. Guessab, I. Mazurenko, E. Lojou, A. de Poulpiquet

{"title":"Electrochemistry of redox enzymes: from functional enzyme immobilization to enzymatic bio-electrochemical devices (tutorial)","authors":"A. Guessab, I. Mazurenko, E. Lojou, A. de Poulpiquet","doi":"10.1016/j.bioelechem.2026.109265","DOIUrl":"10.1016/j.bioelechem.2026.109265","url":null,"abstract":"<div><div>Electrochemistry of redox enzymes is a multidisciplinary field, and students who join labs specialized in bio-electrochemistry have various backgrounds: chemistry, biology, biotechnologies, nanotechnologies, physics, etc., so that their level in the involved disciplines is often heterogeneous, and they sometimes lack the basic knowledge about either of the fields concerned by their project (physical chemistry, electrochemistry, enzymology, etc.). Projects that gather experienced researchers from these different areas also sometimes suffer from a lack of understanding between the partners. This tutorial, which follows a lecture given at the first winter school of the French group of bio-electrochemistry (GFB), aims at guiding newcomers in the field and provides advice for more in-depth and specialized literature. It seeks to provide a solid theoretical and experimental foundation. Redox enzymes and the basic methods of immobilization at the electrode are introduced. The mechanisms of direct and mediated electron transfers are explained. An important electrochemical method, cyclic voltammetry, is explained, and the article describes how to extract information about enzyme/electrode and catalytic reactions. Finally, the tutorial presents two enzymatic bio-electrochemical devices, enzymatic fuel cells and enzymatic biosensors, and provides a guide for their electrochemical characterization.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"171 ","pages":"Article 109265"},"PeriodicalIF":4.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0