Bioelectrochemistry最新文献

Effects of multi-microplastic mixtures on the performance of constructed wetland microbial fuel cells for wastewater treatment 多种微塑料混合物对污水处理人工湿地微生物燃料电池性能的影响

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-19 DOI: 10.1016/j.bioelechem.2025.109008

Shentan Liu , Lang He , Xiaojuan Feng

{"title":"Effects of multi-microplastic mixtures on the performance of constructed wetland microbial fuel cells for wastewater treatment","authors":"Shentan Liu , Lang He , Xiaojuan Feng","doi":"10.1016/j.bioelechem.2025.109008","DOIUrl":"10.1016/j.bioelechem.2025.109008","url":null,"abstract":"<div><div>This study examined the effects of microplastics (MPs) on constructed wetland (CW) and constructed wetland microbial fuel cell (CW-MFC) with different configurations. Four mixed MP types including polyethylene, polypropylene, polystyrene, and polyvinyl chloride were introduced. Planted CW-MFC demonstrated the highest MP removal efficiency of 96.7 % and power density of 14.90 mW m<sup>−2</sup>, outperforming both unplanted CW-MFC and conventional CW. MPs had minimal impact on COD removal, but the removal efficiencies of NH<sub>4</sub><sup>+</sup>-N and TN were significantly inhibited, with TN removal decreasing by approximately 20 % compared to MP-free conditions. TP removal initially decreased but later increased, remaining slightly lower than pre-MP levels. Reduced chlorophyll content in plant leaves indicated MP-induced stress on plant growth. Microbial analysis revealed dominant phyla including <em>Proteobacteria</em>, <em>Patescibacteria</em>, and <em>Bacteroidota</em> contributed to nitrogen removal. In planted systems, genera such as <em>Denitratisoma</em>, <em>Sulfuritalea</em>, and <em>Endomicrobium</em> contributed to denitrification. In CW-MFCs, <em>Geobacter</em> and <em>Candidatus Falkowbacteria</em> dominated, with <em>Geobacter</em> linked to electricity generation and <em>Candidatus Falkowbacteria</em> associated with carbon and nitrogen cycles. MPs negatively affected denitrification by suppressing key denitrifiers such as <em>Denitratisoma</em> but enhanced electricity generation by enriching electroactive bacteria like <em>Geobacter</em>. These findings reveal complex MP-driven interactions influencing microbial communities and system performance.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 109008"},"PeriodicalIF":4.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ammonia recovery from waste streams: Energy efficiency and performance of microbial electrochemical system and electrochemical system 废水中氨的回收：微生物电化学系统和电化学系统的能量效率和性能

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-19 DOI: 10.1016/j.bioelechem.2025.109007

Dileep Kumar Yeruva, Irini Angelidaki

{"title":"Ammonia recovery from waste streams: Energy efficiency and performance of microbial electrochemical system and electrochemical system","authors":"Dileep Kumar Yeruva, Irini Angelidaki","doi":"10.1016/j.bioelechem.2025.109007","DOIUrl":"10.1016/j.bioelechem.2025.109007","url":null,"abstract":"<div><div>Nutrient recovery from waste streams, particularly digestate from anaerobic digestion, represents a promising strategy for sustainable waste management and circular economy practices. This study evaluated the performance of electrochemical (ES) and Microbial electrochemical systems (MES) for ammonia recovery from digestate under varying voltages. Energy consumption, and natural pH increase in catholyte were investigated for increased recovery efficiency. The results demonstrate that MES and ES exhibited similar recovery rates, with ammonia recovery reaching approx. 49 % at 1.2 V, while at lower energy efficiency MES outperformed ES. At ‘0’ voltage, MES achieved a baseline recovery of 31.85 %, significantly higher than ES (13.25 %), highlighting the contribution of microbial electrochemical activity in driving nutrient recovery with minimal energy input. MES exhibited remarkably lower energy consumption, requiring nine-fold less energy than ES at low applied voltages, owing to the synergistic effects of microbial activity and bioelectricity generation. Moreover, MES minimized ammonium transport losses and showed better stability in cathodic pH variations, favouring long-term operational viability. The findings in MES as a more sustainable alternative for ammonia recovery, emphasizing its potential to global carbon neutrality and operational costs in nutrient recycling processes.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 109007"},"PeriodicalIF":4.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent development in metal-organic frameworks-based electrochemical aptasensors for detection of cancer biomarkers 基于金属-有机框架的电化学感应传感器检测癌症生物标志物的研究进展

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-16 DOI: 10.1016/j.bioelechem.2025.109006

Imamdin Chandio , Sanam Rahujo , Zaheer Ahmed Chandio , Md Abdur Rashid , Misbah Ur Rehman , Zahid H. Shar , Khalid Hussain Thebo

{"title":"Recent development in metal-organic frameworks-based electrochemical aptasensors for detection of cancer biomarkers","authors":"Imamdin Chandio , Sanam Rahujo , Zaheer Ahmed Chandio , Md Abdur Rashid , Misbah Ur Rehman , Zahid H. Shar , Khalid Hussain Thebo","doi":"10.1016/j.bioelechem.2025.109006","DOIUrl":"10.1016/j.bioelechem.2025.109006","url":null,"abstract":"<div><div>Aptasensors utilize aptamers as recognition elements, which offer high sensitivity, selectivity, ease of modification, outstanding stability, real-time detection, biocompatibility, cost-effectiveness, and good integration. Utilizing metal-organic framework (MOF)-based electrochemical aptasensors is a promising approach that is drawing significant attention. MOF-based aptasensors for cancer detection present a promising avenue, yet they face several challenges. The optimization of sensitivity and selectivity in the MOF-aptamer system is complex, requiring a delicate balance to distinguish the target analyte from interfering substances in physiological environments. Efficient immobilization of aptamers on MOF surfaces while maintaining their conformation and stability under physiological conditions poses a crucial challenge for robust sensing. Integrating MOFs and aptamers with transduction systems, such as electrodes in electrochemical sensors, is critical for achieving efficient signal transduction and maintaining sensor stability. Herein, the latest developments in MOF-based electrochemical aptasensors for detecting tumor markers will be discussed. Focus will be given on single metallic, bimetallic, and calcinated-based MOFs and their strengths and weaknesses will be summarized. Further, the synthesis strategy of electrochemical sensors is analyzed to meet the requirements of selectivity and sensitivity. Finally, the future perspectives for developing and applying electrochemical aptasensors are also discussed.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 109006"},"PeriodicalIF":4.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nernst-Michaelis-Menten framework unlocks electrochemical kinetics for laccases Nernst-Michaelis-Menten框架揭示了漆酶的电化学动力学

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-13 DOI: 10.1016/j.bioelechem.2025.109003

Elise Martin , Fabrice Audonnet , Daniel Yaacoub , Pascal Dubessay , Philippe Michaud

{"title":"Nernst-Michaelis-Menten framework unlocks electrochemical kinetics for laccases","authors":"Elise Martin , Fabrice Audonnet , Daniel Yaacoub , Pascal Dubessay , Philippe Michaud","doi":"10.1016/j.bioelechem.2025.109003","DOIUrl":"10.1016/j.bioelechem.2025.109003","url":null,"abstract":"<div><div>Determining oxidoreductase kinetic parameters remains challenging due to spectrophotometric method limitations. Here, we present an innovative approach combining electrochemistry and enzymology principles through a novel Nernst-Michaelis-Menten theoretical framework. This model merges the Nernst equation, describing electrochemical equilibrium, with Michaelis-Menten kinetics, enabling accurate enzyme parameters determination, via chronopotentiometry. Using a commercial laccase from <em>Trametes versicolor</em> as a model system, we demonstrate precise kinetic parameters measurement for both chromophoric (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS, K<sub>m</sub> = (56.7 ± 6.3) μM) and non-chromophoric (hydroquinone, K<sub>m</sub> = (196 ± 59) μM) substrates, validated against established techniques. The method requires minimal enzyme quantities and enables rapid analysis. This approach overcomes current methodological limitations and extends to other oxidoreductases, providing a powerful tool for enzyme characterization. Our work provides a new paradigm for enzyme kinetics, expanding the scope of analysable enzymatic systems, including those that were previously challenging to characterize with conventional methods.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 109003"},"PeriodicalIF":4.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of glioma spheroid viability and metastatic potential following monophasic and biphasic pulsed electric fields 单相和双相脉冲电场对胶质瘤球体活力和转移潜能的影响

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-13 DOI: 10.1016/j.bioelechem.2025.109005

Julio P. Arroyo , Edward J. Jacobs , Raffae N. Ahmad , Ashil J. Amin , Scott S. Verbridge , Rafael V. Davalos

{"title":"Characterization of glioma spheroid viability and metastatic potential following monophasic and biphasic pulsed electric fields","authors":"Julio P. Arroyo , Edward J. Jacobs , Raffae N. Ahmad , Ashil J. Amin , Scott S. Verbridge , Rafael V. Davalos","doi":"10.1016/j.bioelechem.2025.109005","DOIUrl":"10.1016/j.bioelechem.2025.109005","url":null,"abstract":"<div><div>Currently, the leading 3D cell culture models for characterizing and validating pulsed electric fields (PEFs) are spheroids and cell-laden hydrogels. We hypothesize that incorporating a glioblastoma multicellular tumor spheroid (MTS) onto a collagen hydrogel will leverage their strengths to form a more physiologically relevant model to study viability, proliferation, and migration. The MTS-hydrogel platform was subjected to PEFs varying in pulse width and electric field (EF) strength. Treated MTS were monitored and evaluated for viability and proliferation (Live/Dead imaging, XTT Cell Viability Assay), and migration (brightfield imaging) over 5 days post-treatment. In vitro experimentation was validated with a multi-layered spheroid finite element model, evaluating transmembrane potential (TMP), pore density, and pore formation across the spheroid layers. MTS exposed to longer pulse widths (5, 100 μs) and higher EFs (2000, 2500 V/cm) experienced a complete ablation. Smaller pulse widths and lower EFs produced partial ablations initially reducing the MTS, but unable to prevent the MTS from recuperating. Similarly shown with the computational model, a TMP was accomplished through the MTS, inducing electroporation; however, pore formation was dictated by the increase in pulse width and EF beyond the superficial layer. EFs of 2000 V/cm and above severely constrained the migration independent of pulse width.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 109005"},"PeriodicalIF":4.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemiluminescent/colorimetric biosensors for ultrasensitive detection of Aβ based on CdS quantum dot-modulated HOFs-Cu2+-g-C₃N₄ 基于CdS量子点调制hfs - cu2 +-g-C₃N₄超灵敏检测Aβ的电化学发光/比色生物传感器

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-13 DOI: 10.1016/j.bioelechem.2025.109004

Yingru Tang , Kangli Xing , Jiayu Wan, Xinhao Wang, Yinan Li, Fang Li, Xiaoyan Yang

{"title":"Electrochemiluminescent/colorimetric biosensors for ultrasensitive detection of Aβ based on CdS quantum dot-modulated HOFs-Cu2+-g-C₃N₄","authors":"Yingru Tang , Kangli Xing , Jiayu Wan, Xinhao Wang, Yinan Li, Fang Li, Xiaoyan Yang","doi":"10.1016/j.bioelechem.2025.109004","DOIUrl":"10.1016/j.bioelechem.2025.109004","url":null,"abstract":"<div><div>In this work, we presented an electrochemiluminescent (ECL)/colorimetric biosensors for ultrasensitive detection of <em>β-</em>amyloid peptide (A<em>β</em>) based on hydrogen-bonded organic framework (HOFs)-Cu<sup>2+</sup>-graphitic carbon nitride (g-C<sub>3</sub>N<sub>4</sub>) nanocomposites. HOFs-Cu<sup>2+</sup>-g-C<sub>3</sub>N<sub>4</sub> could be used both as an ECL luminescent substrate and as a nanozyme to catalyze the chromogenic reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). Specifically, in the ECL mode, HOFs-Cu<sup>2+</sup>-g-C<sub>3</sub>N<sub>4</sub> were firstly loaded on the surface of the electrode. The target analyte A<em>β</em> could simultaneously bind both Cu<sup>2+</sup> and the peptide fragment KLVFF, thereby capturing the CdS quantum dots (QDs)-KLVFF on the electrode. The CdS QDs could perform ECL resonance energy transfer with HOFs-Cu<sup>2+</sup>-g-C<sub>3</sub>N<sub>4</sub>, resulting the increase in the ECL intensity. Meanwhile, in the colorimetric mode, the aptamer loaded on the magnetic beads could specifically capture the target A<em>β</em>. HOFs-Cu<sup>2+</sup>-g-C<sub>3</sub>N<sub>4</sub> used as the nanozyme in the TMB-H<sub>2</sub>O<sub>2</sub> chromogenic system could be sequentially bound with the target A<em>β</em>. The convenient colorimetric detection of A<em>β</em> was achieved after magnetically separating using the functionalized magnetic beads. The concentration range of A<em>β</em> detected by ECL mode was 0.1 pM ∼ 0.1 μM with a detection limit of 0.07 pM, while the concentration range of A<em>β</em> detected by colorimetric assay was 0.1 pM ∼ 0.1 μM with a detection limit of 0.032 pM. These two different assay modes enhanced the sensitivity and reliability of the detection of A<em>β</em> and demonstrated potential applicability in the early diagnosis of Alzheimer's disease.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 109004"},"PeriodicalIF":4.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-electrode electrochemical scans vs. three-electrode scans in microbiologically influenced corrosion detection with various counter electrode/pseudo-reference electrode materials 两电极电化学扫描与三电极扫描在各种对电极/伪参比电极材料的微生物影响腐蚀检测

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-13 DOI: 10.1016/j.bioelechem.2025.109002

Lingjun Xu , Adnan Khan , Di Wang , Pruch Kijkla , Sith Kumseranee , Suchada Punpruk , Sarah A. Aqeel , Tingyue Gu

{"title":"Two-electrode electrochemical scans vs. three-electrode scans in microbiologically influenced corrosion detection with various counter electrode/pseudo-reference electrode materials","authors":"Lingjun Xu , Adnan Khan , Di Wang , Pruch Kijkla , Sith Kumseranee , Suchada Punpruk , Sarah A. Aqeel , Tingyue Gu","doi":"10.1016/j.bioelechem.2025.109002","DOIUrl":"10.1016/j.bioelechem.2025.109002","url":null,"abstract":"<div><div>Electrochemical measurements are popular in lab microbiologically influenced corrosion (MIC) studies. A classical 3-electrode (3E) setup is not suitable for biofilm/MIC test kits use because the reference electrode (RE) is fragile. For biofilm/MIC test kit applications, the counter electrode (CE) is used as CE and also pseudo-RE (p-RE) to create a 2-electrode (2E) setup. This work compared 2E and 3E scan results in a 450 mL glass cell containing sulfate reducing bacteria (SRB) with platinum and graphite serving as CE materials. In both SRB incubation and injection tests, 2E scan results turned out to be as reliable as 3E scan results in reflecting corrosion rates. The chemical injection test with platinum CE showed that after riboflavin, magnetite nanoparticles and tetrakis hydroxymethyl phosphonium sulfate biocide injections, the polarization resistance of X65 WEs shifted by −8.8 %, −6.2 % and 11 %, respectively in 3E scan, and − 6.6 %, −7.1 % and 8.3 %, respectively in 2E scan. The 2E and 3E scan results in a 10 mL miniature glass cell also correctly reflected MIC information indicating SRB biofilm maturity at 3 d. This study found that 316L SS, Cr, Ni and Mo can be potentially used as additional choices for CE/p-RE materials in biofilm/MIC test kit.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 109002"},"PeriodicalIF":4.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance evaluation of Iron-doped carbon cloth cathodes in bio-electrochemical system for dye degradation 掺杂铁炭布阴极在生物电化学系统中降解染料的性能评价

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-09 DOI: 10.1016/j.bioelechem.2025.109001

Juliana John, Haribabu Krishnan, Karnapa Ajit

{"title":"Performance evaluation of Iron-doped carbon cloth cathodes in bio-electrochemical system for dye degradation","authors":"Juliana John, Haribabu Krishnan, Karnapa Ajit","doi":"10.1016/j.bioelechem.2025.109001","DOIUrl":"10.1016/j.bioelechem.2025.109001","url":null,"abstract":"<div><div>Bio electro-Fenton (BEF) is an advanced bio-electrochemical system that utilizes the synergistic effect of microbial activity and electrochemical reactions to degrade organic pollutants in wastewater. This study investigates the degradation of Chrysoidine Y dye using iron-doped, binder-free carbon cloth cathodes in a BEF system. The cathodes exhibited significant electrochemical enhancements, including increased electrochemical active surface area, improved electrical conductivity, and efficient Fe<sup>3+</sup>/Fe<sup>2+</sup> redox cycling. Under optimal conditions (1000 mg/L substrate concentration, pH 3, 750 Ω applied resistance and 20 ppm dye concentration), the BEF system achieved 73.14 % dye removal and 58.69 % total organic carbon (TOC) reduction within 10 h. Gas Chromatography-Mass Spectrometry (GC–MS) analysis revealed the breakdown of chrysoidine Y into non-toxic intermediates. Toxicity tests using chick pea germination showed no adverse effects (Germination Index>80 %) in the treated effluent. Whole genome sequencing of the anodic biofilm identified electrogenic bacteria responsible for electron generation to drive the fenton reaction. These findings highlight the potential of iron-doped cathodes in BEF systems for sustainable and efficient treatment of dye-contaminated wastewater, ensuring effective pollutant removal and environmental safety.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 109001"},"PeriodicalIF":4.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144069302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Sensitive Label-Free Immunosensor based on 5-Sulfosalicylic Acid Doped Poly(2-chloroaniline) for Detection of CA72–4 in Gastric Cancer 基于5-磺基水杨酸掺杂聚2-氯苯胺的高灵敏度无标记免疫传感器检测胃癌CA72-4

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-08 DOI: 10.1016/j.bioelechem.2025.108997

Natthaporn Thongwattana , Nuttha Ariyasajjamongkol , Napa Parinyanitikul , Manit Nithitanakul , Katesara Phasuksom , Anuvat Sirivat

{"title":"Highly Sensitive Label-Free Immunosensor based on 5-Sulfosalicylic Acid Doped Poly(2-chloroaniline) for Detection of CA72–4 in Gastric Cancer","authors":"Natthaporn Thongwattana , Nuttha Ariyasajjamongkol , Napa Parinyanitikul , Manit Nithitanakul , Katesara Phasuksom , Anuvat Sirivat","doi":"10.1016/j.bioelechem.2025.108997","DOIUrl":"10.1016/j.bioelechem.2025.108997","url":null,"abstract":"<div><div>Gastric cancer (GC) is a leading cause of cancer-related deaths worldwide, highlighting the urgent need for sensitive and cost-effective diagnostic tools. In this work, a label-free electrochemical immunosensor (ECI) based on poly(2-chloroaniline) doped with 5-sulfosalicylic acid (dP2ClAn/SSA) was developed for the detection of cancer antigen 72–4 (CA72–4), a key biomarker for the early GC diagnosis. The incorporation of 3-aminopropyltriethoxysilane (APTES) as a linker agent facilitated the antibody immobilization, improved electron transfer, and promoted stable sensor performance. Detection of CA72–4 using differential pulse voltammetry (DPV) demonstrated a broad detection range of 0.001–500 U/mL, with the calculated limit of detection of 0.0053 U/mL (1 U = 1 ng). The sensor exhibited excellent selectivity, reproducibility, and stability over 28 days of storage. Validation with human serum samples confirmed its accuracy and reliability, with performance comparable to ELISA. With its simplicity, portability, and cost-effectiveness, this ECI represents a promising diagnostic tool for early GC detection and clinical point-of-care applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108997"},"PeriodicalIF":4.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective butyrate production from CO2 and methanol in microbial electrosynthesis - influence of pH 微生物电合成CO2和甲醇选择性丁酸盐——pH的影响

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-06 DOI: 10.1016/j.bioelechem.2025.109000

Hui Yao , Meritxell Romans-Casas , Igor Vassilev , Johanna M. Rinta-Kanto , Sebastià Puig , Antti J. Rissanen , Marika Kokko

{"title":"Selective butyrate production from CO2 and methanol in microbial electrosynthesis - influence of pH","authors":"Hui Yao , Meritxell Romans-Casas , Igor Vassilev , Johanna M. Rinta-Kanto , Sebastià Puig , Antti J. Rissanen , Marika Kokko","doi":"10.1016/j.bioelechem.2025.109000","DOIUrl":"10.1016/j.bioelechem.2025.109000","url":null,"abstract":"<div><div>Methanol assisted microbial electrosynthesis (MES) enables butyrate production from carbon dioxide and methanol using external electricity. However, the effects of operational parameters on butyrate formation remain unclear. By running three flat plate MES reactors with fed-batch mode at three controlled pH values (5.5, 6 and 7), the present study investigated the influence of pH on methanol assisted MES by comparing the process performance, microbial community structure, and genetic potential. The highest butyrate selectivity (87 % on carbon basis) and the highest butyrate production rate of 0.3 g L<sup>−1</sup> d<sup>−1</sup> were obtained at pH 6. At pH 7, a comparable butyrate production rate was achieved, yet with a lower selectivity (70 %) accompanied with acetate production. Butyrate production rate was considerably hindered at pH 5.5, reaching 0.1 g L<sup>−1</sup> d<sup>−1</sup>, while the selectivity reached was up to 81 %. Methanol and CO<sub>2</sub> consumption increased with pH, along with more negative cathodic potential and more negative redox potential. Furthermore, pH affected the thermodynamical feasibility of involved reactions. The results of metagenomic analyses suggest that <em>Eubacterium callanderi</em> dominated the microbial communities at all pH values, which was responsible for methanol and CO<sub>2</sub> assimilation via the Wood-Ljungdahl pathway and was likely the main butyrate producer via the reverse β-oxidation pathway.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 109000"},"PeriodicalIF":4.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0