Bioelectrochemistry最新文献

{"title":"A high-sensitivity ECL biosensor for single-cell analysis: Integrating CRISPR/Cas12a and entropy-driven amplification","authors":"Jiaying Wang ,&nbsp;Yu Tian ,&nbsp;Kai Zhang ,&nbsp;Yi Zhao","doi":"10.1016/j.bioelechem.2025.109045","DOIUrl":"10.1016/j.bioelechem.2025.109045","url":null,"abstract":"<div><div>This study presents a homogeneous electrochemiluminescence (ECL) biosensor for ultrasensitive detection of HEK293 cells by targeting the hERG potassium channel at the single-cell level. The biosensor integrates multiple signal amplification steps, including photocleavable DNA–antibody conjugates, entropy-driven strand displacement, T7 RNA polymerase-mediated transcription, and CRISPR/Cas12a-mediated trans-cleavage. This cascade enables precise and robust signal enhancement. A key feature of the system is its ability to generate a clearly measurable ECL response from as little as a single HEK293 cell, without the need for signal averaging. Specificity was confirmed using unrelated cell lines and mismatched DNA sequences, with minimal background observed in negative controls. Optimization of key parameters—such as enzyme concentrations, reaction times, and duplex composition—ensured consistent and reproducible performance. ECL measurements were conducted under a defined voltage scan (0–1.3 V) in a three-electrode system, and emission signals were recorded using a photomultiplier tube. This biosensor demonstrates the feasibility of single-cell detection with high sensitivity and specificity, offering a promising platform for future applications in cell-based analysis and molecular diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109045"},"PeriodicalIF":4.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rolling circle amplification and CRISPR/Cas14a with nanozyme for electrochemical detecting miRNA-205 in NPC-derived exosomes","authors":"Xueqian Xiao ,&nbsp;Ying Lu ,&nbsp;Jianxue Zhang ,&nbsp;Wei Ni ,&nbsp;Junlin Liu ,&nbsp;Chaoqing Li ,&nbsp;Qunfeng Yao ,&nbsp;Yujie Sun ,&nbsp;Guo-jun Zhang ,&nbsp;Yulin Zhang ,&nbsp;Yuqing Qin ,&nbsp;Yajuan Zhou","doi":"10.1016/j.bioelechem.2025.109046","DOIUrl":"10.1016/j.bioelechem.2025.109046","url":null,"abstract":"<div><div>Nasopharyngeal carcinoma (NPC) is a highly malignant tumor, and early detection of biomarkers like miRNA-205 (miR-205) is critical for improving prognosis. However, accurate detection of miR-205 remains challenging due to its low abundance and matrix interference. Herein, an ultrasensitive electrochemical biosensor integrating Pt nanowires/MXene (PtNWs/MXene), rolling circle amplification (RCA), and CRISPR/Cas14a was developed for detecting exosomal miR-205. The dual characteristics of PtNWs/MXene (differential adsorption capacity for intact and cleaved DNA; HRP-like nanozyme activity) enable the conversion of the miR-205-triggered RCA-Cas14a cascade reaction into significant electrochemical signal changes. This biosensor eliminates the requirement for signal probe labeling of the electrode-modified DNA. Moreover, the enzyme-mimicking catalytic activity of PtNWs/MXene enables the catalysis of numerous 3,3′,5,5′-tetramethylbenzidine (TMB) molecules, realizing a “one-to-many” signal amplification effect that significantly improves detection sensitivity. The biosensor achieves a detection limit of 4.6 aM (50 aM-10 pM linear range) and distinguishes single-base mismatches. Clinical validation confirmed its ability to differentiate NPC patients from healthy individuals, aligning with qRT-PCR results. By adjusting the RCA template, this strategy can be adapted for diverse RNA/DNA targets, offering a versatile platform for early disease diagnosis.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109046"},"PeriodicalIF":4.8,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of amoxicillin and silver metal in combating bacterial infections: A novel approach to overcoming antibiotic resistance","authors":"A. Berd ,&nbsp;L. El Haddioui ,&nbsp;B. Chhaibi ,&nbsp;A. Hrioua ,&nbsp;F. Laghrib ,&nbsp;M. Bakasse ,&nbsp;S. Lahrich ,&nbsp;A. Farahi ,&nbsp;M.A. El Mhammedi ,&nbsp;S. Saqrane","doi":"10.1016/j.bioelechem.2025.109044","DOIUrl":"10.1016/j.bioelechem.2025.109044","url":null,"abstract":"<div><div>This study focuses on the synthesis of the compounds based amoxicillin and silver, while highlighting their increased efficacy against bacterial infections and their potential to fight antibiotic resistance. The synergy has been achieved in two forms: an amoxicillin‑silver complex (AMX<img>Ag) and amoxicillin-stabilized silver nanoparticles (AMX-AgNPs). The interaction between amoxicillin and silver ions (Ag⁺) was investigated using electrochemical and spectroscopic methods. The synthesized AMX-Ag complex and AMX-AgNPs were thoroughly characterized using a range of physicochemical techniques to determine their structural and functional properties. The UV–visible result confirmed the formation of the coordination compound, which is consistent with the electrochemical data that showed changes in the oxidation peak of AMX in the presence of Ag(I) ions. The XRD analysis of the synthesized AMX-AgNPs revealed a face-centered cubic (fcc) lattice structure.</div><div>Their antibacterial efficacy was then evaluated against <em>Escherichia coli</em> (<em>E. coli</em>) through the agar diffusion method. The results revealed that both the AMX-Ag complex and AMX-AgNPs exhibited a marked enhancement in bactericidal activity compared to amoxicillin alone. This suggests that these formulations hold significant promise as alternative therapeutic strategies for combating <em>E. coli</em> infections, particularly those resistant to conventional antibiotic treatments.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109044"},"PeriodicalIF":4.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical aptasensors based on hydrogen-bonded organic frameworks for detecting trace penicillin G","authors":"Yatian Zhao ,&nbsp;Yanni Peng ,&nbsp;Yanhui Liu ,&nbsp;Ruixuan Li ,&nbsp;Hongming He ,&nbsp;Cheng-Peng Li ,&nbsp;Cheng Wei ,&nbsp;Feng Guo ,&nbsp;Wenrong Wei","doi":"10.1016/j.bioelechem.2025.109041","DOIUrl":"10.1016/j.bioelechem.2025.109041","url":null,"abstract":"<div><div>This study presents the rational design and synthesis of two hydrogen-bonded organic frameworks (HOFs) via hydrogen-bonding-driven self-assembly of organic monomers. HOFs have abundant binding sites and extended π-conjugated networks for aptamer immobilization. Compared to non-porous HOF-BPTCA, the porous HOF-TCBP can synergistically enhance both electronic conductivity and accessible functional area to fabricate a high-performance electrochemical aptasensor. Penicillin G (PG) is a widely used broad-spectrum antibiotic, which is selected to investigate the sensing ability of aptasensors. The electrochemical aptasensor based on HOF-TCBP can quantitatively detect trace PG using differential pulse voltammetry (DPV), which exhibits a good linear range from 0.0005 ng mL⁻¹ to 0.5 ng mL⁻¹ and a low limit of detection (1.04 fg mL⁻¹). This work opens a novel idea for constructing high-performance HOFs-based electrochemical aptasensors and expands their applications in the electrical biosensing field.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109041"},"PeriodicalIF":4.8,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platinum–zinc oxide as a super signal amplifier for ultrasensitive electrochemical immunodetection of CEA","authors":"Xinru Yuan ,&nbsp;Zixin Chen ,&nbsp;Yuxuan Li ,&nbsp;Jiarui Zhang ,&nbsp;Wenxin Li ,&nbsp;Huimin Li ,&nbsp;Ziyi Lin ,&nbsp;Yixin Zheng ,&nbsp;Shasha Zheng ,&nbsp;Jinting Meng ,&nbsp;Yingwei Zhang ,&nbsp;Heyun Shen","doi":"10.1016/j.bioelechem.2025.109037","DOIUrl":"10.1016/j.bioelechem.2025.109037","url":null,"abstract":"<div><div>Electrochemical immunosensors are essential for disease screening, diagnosis, treatment and monitoring. In this study, a novel sandwich-type electrochemical immunosensor has been constructed for the sensitive detection of tumor marker carcinoembryonic antigen (CEA) based on platinum‑zinc oxide (Pt-ZnO) nanoparticles as a signal amplification platform. Pt-ZnO has outstanding peroxidase-like activity that enables efficient catalysis of the H₂O₂ reaction, leading to effective amplification of the current signal through numerous electron transfers. Its high specific surface area and excellent dispersion allow for immobilization with secondary antibodies through the Pt<img>N bond. Moreover, graphene quantum dots–chitosan possesses outstanding electrical conductivity and biocompatibility, enabling it to improve interfacial electron transfer and capture more primary antibodies on its surface. The Pt-ZnO signal amplification system has achieved a wide linear range for the CEA detection, which spanned from 10 fg mL⁻¹ to 100 ng mL⁻¹ with the limit of detection (LOD) of 1.02 fg mL⁻¹. The LOD of the electrochemical immunosensor was seven orders of magnitude lower than that of the traditional enzyme-linked immunosorbent assay. Furthermore, it has viable reproducibility, stability and practicality. Hence the Pt-ZnO electrochemical immunosensor has great potential for the clinical identification of CEA.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109037"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resolving Shewanella vesicular nanowire structure during microbial extracellular electron transfer to a poised electrode","authors":"Nitesh Kanojia ,&nbsp;Jordan Poitras ,&nbsp;Thomas Jones ,&nbsp;Bernardino Virdis ,&nbsp;Gordon Southam ,&nbsp;Lucinda Elizabeth Doyle","doi":"10.1016/j.bioelechem.2025.109039","DOIUrl":"10.1016/j.bioelechem.2025.109039","url":null,"abstract":"<div><div>The nanowires of the model electroactive bacterium <em>Shewanella oneidensis</em> have been the subject of numerous studies to elucidate their structure and function. These previous reports have elegantly utilised advanced microscopic techniques to investigate nanowires formed in response to oxygen limitation. However, the detailed structure of nanowires formed on electrodes during extracellular electron transfer has not been reported and it is imperative to determine whether they possess the same vesicular structure that has been reported in the absence of extracellular electron transfer. Using an acetone hexamethyldisilazane dehydration method to preserve soft biological materials, we employed the relatively uncomplicated technique of secondary electron field emission-scanning electron microscopy to visualise the vesicular nanowire structure while attached to an electrode from an operating bioelectrochemical system. Early-stage nanowires appear to consist of intact chains of outer-membrane vesicles forming connections with the electrode surface and with neighbouring cells. Relying on secondary electrons from the inherently conductive carbon felt electrode, sputter coating could be avoided and the delicate structure of the vesicles was preserved with increased detail. The findings inform the fundamental understanding of nanowires during electron transfer and the simple protocol will allow their examination on a variety of existing and emerging electrode materials.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109039"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the analytical potential of thin organic film electrodes: Electrochemical insights into anticancer drug docetaxel at liquid|liquid interfaces","authors":"Maryia-Mazhena Dzemidovich ,&nbsp;Andrzej Leniart ,&nbsp;Simona Baluchová ,&nbsp;Sławomira Skrzypek ,&nbsp;Valentin Mirceski ,&nbsp;Mariola Brycht","doi":"10.1016/j.bioelechem.2025.109040","DOIUrl":"10.1016/j.bioelechem.2025.109040","url":null,"abstract":"<div><div>A thin organic film electrode (TOFE) system was employed for the indirect electrochemical investigation of docetaxel (DTX), an anticancer drug from the class of mitotic inhibitors. The TOFE consists of a thin membrane immobilized on a carbon electrode substrate, composed of a water-immiscible organic solvent that forms a stable liquid│liquid interface upon immersion in an aqueous electrolyte. Due to the high oxidation potential of DTX at solid electrodes, an alternative detection strategy was adopted based on the interaction between DTX and the liquid|liquid interface of the TOFE. Systematic optimization of the organic and aqueous phase compositions, as well as the electrode material, was first performed in the absence of DTX to ensure reliable and reproducible conditions at the interface. The optimized TOFE system, based on nitrobenzene as the organic solvent, perchlorate anions as the supporting electrolyte, and an edge-plane pyrolytic graphite as electrode material, provided the best performance. The optimal electrochemical response was also observed when perchlorate anions were present in the aqueous phase, while the nature of the cations in the aqueous phase had negligible influence. The optimized TOFE system was then used to study the effect of DTX on ion transfer processes at the liquid│liquid interface, where a pronounced, concentration-dependent inhibitory effect was observed. Electrochemical investigations using cyclic voltammetry and square-wave voltammetry (SWV), including frequency- and amplitude-dependent analyses, demonstrated that DTX adsorbs to the membrane│water interface, forming blocking layer that markedly slowing ion transfer and displacing the quasi-reversible maxima beyond the instrumentally accessible window. The system exhibited a linear analytical response in the concentration range of 10.0–100.0 μmol L⁻¹ DTX, with low detection and quantification limits when analyzed by SWV. The LOD values were 3.27 μmol L⁻¹ (anodic) and 3.26 μmol L⁻¹ (cathodic), while the LOQ were 9.90 μmol L⁻¹ and 9.89 μmol L⁻¹, respectively. These findings validate the TOFE platform as a robust and versatile tool for the indirect detection of redox-inactive pharmaceutical compounds in biphasic electrochemical systems. Given the key role of mitotic inhibitors like DTX in cancer therapy, studying their behavior at biomimetic interfaces may advance understanding of drug–membrane interactions and support the development of improved delivery and diagnostic strategies.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109040"},"PeriodicalIF":4.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a CRISPR/Cas13a-based electrochemiluminescence biosensing strategy for sensitive detection of α-synuclein oligomers in neurodegenerative diseases","authors":"Yuanxun Gong ,&nbsp;Guangsheng Li ,&nbsp;Yiqiu Meng ,&nbsp;Jihua Wei ,&nbsp;Qianli Tang ,&nbsp;Longjian Huang ,&nbsp;Kai Zhang ,&nbsp;Xianjiu Liao","doi":"10.1016/j.bioelechem.2025.109038","DOIUrl":"10.1016/j.bioelechem.2025.109038","url":null,"abstract":"<div><div>In this study, we report a highly sensitive CRISPR/Cas13a-based electrochemiluminescence (ECL) biosensor for detecting α-synuclein oligomers, early biomarkers for neurodegenerative diseases. The system integrates aptamer recognition, T7 transcription, CRISPR/Cas13a cleavage, and EXPAR amplification. α-Synuclein binding triggers the release of the T7 promoter, leading to RNA production that activates Cas13a, initiating collateral cleavage and EXPAR, generating double-stranded DNA that interacts with [Ru(phen)₂dppz]²⁺ to produce a measurable ECL signal. The sensor achieved an ultralow detection limit of 1.025 aM with high specificity and stability. In serum samples, recovery ranged from 95.2 % to 99.8 %, demonstrating strong accuracy. No interference was observed from unrelated proteins. The biosensor showed excellent reproducibility (intra-day RSD = 0.78 %, inter-day RSD = 2.86 %) and stable performance over 14 days. Compared to other existing methods, this strategy offers superior sensitivity and comparable dynamic range, making it highly suitable for clinical use. Although the assay requires multiple steps and approximately two hours, the tradeoff is justified by its performance. This work highlights the potential of combining CRISPR/Cas13a with ECL for ultra-sensitive biomarker detection in complex samples, supporting early diagnosis and monitoring of Parkinson's and Alzheimer's disease.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109038"},"PeriodicalIF":4.8,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-frequency irreversible electroporation suppresses invasion and metastasis by targeting SIRT1/2 in highly invasive tumor cells: an in vitro study","authors":"Xueya Wang ,&nbsp;Tianle Hong ,&nbsp;Gengliang Liu ,&nbsp;Junfeng Rao ,&nbsp;Fukun Shi ,&nbsp;Haifeng Wang ,&nbsp;Jinsong Guo ,&nbsp;Jie Zhuang","doi":"10.1016/j.bioelechem.2025.109036","DOIUrl":"10.1016/j.bioelechem.2025.109036","url":null,"abstract":"<div><div>Sirtuin proteins have key roles in cancer progression and metastases. However, their role in bioelectrical modulation via high-frequency irreversible electroporation (H-FIRE) remains poorly defined. This study aims to investigate the molecular mechanism by which H-FIRE suppresses invasion and metastasis in highly invasive cancer cells (U87 and U2OS) through SIRT1/2 downregulation. We systematically assessed H-FIRE effects on cell proliferation, clonogenicity, invasion, metastasis, apoptosis, mitochondrial morphology, and SIRT1/2 expression. Our results demonstrated that H-FIRE significantly suppressed proliferation, invasion, and metastasis in both two cell lines, concurrently inducing mitochondrial fragmentation and apoptosis. Crucially, H-FIRE markedly downregulated SIRT1/2 expression. Notably, SIRT activators partially reversed H-FIRE-mediated invasion suppression, whereas SIRT inhibitors enhanced inhibitory effect. These results underscore that H-FIRE impedes the invasion and metastasis of highly invasive tumor cells through inhibition of SIRT1/2 expression and induction of mitochondrial apoptosis. Our findings establish SIRT1/2 as critical molecular targets mediating the anti-metastatic effect of H-FIRE and support its potential as an anti-metastatic therapy.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109036"},"PeriodicalIF":4.8,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of yeast extract on bacterial metabolism and nickel microbiologically influenced corrosion: Insights into medium optimization and biofilm electron transfer mechanism","authors":"Yanan Pu ,&nbsp;Fan Feng ,&nbsp;Yue Hou ,&nbsp;Su Hou ,&nbsp;Zihao Guo ,&nbsp;Congrui Zhu ,&nbsp;Shougang Chen","doi":"10.1016/j.bioelechem.2025.109027","DOIUrl":"10.1016/j.bioelechem.2025.109027","url":null,"abstract":"<div><div>Yeast extract (YE) serves as a complex nutrient source and plays a pivotal role in the formation and development of microbial biofilms. This work elucidates the critical role of YE in modulating the metabolic activity of <em>Desulfovibrio vulgaris</em>, characteristics of passive films, and the associated microbiologically influenced corrosion (MIC) behavior of nickel (Ni). The presence of YE suppresses corrosion processes linked to extracellular electron transfer (EET) by reducing the necessity for <em>D. vulgaris</em> to directly extract electrons from Ni. In the absence of YE, a greater number of <em>D. vulgaris</em> cells adhere to the Ni surface, forming biofilms with an increased reliance on EET from Ni, thereby exacerbating localized corrosion. This is evidenced by increased weight loss, deeper pitting, and elevated localized corrosion current density, establishing a clear correlation between YE availability and the mitigation of EET-mediated MIC. Meanwhile, YE mitigates EET-driven corrosion by regulating the biofilm structure, facilitating the formation of a protective layer, and modifying the passive film on Ni. A key implication of this work is the reconsideration of YE as a universal nutrient in MIC research, emphasizing the need for caution when using YE in MIC studies that focus on EET-driven mechanisms.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"166 ","pages":"Article 109027"},"PeriodicalIF":4.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}