Bioelectrochemistry最新文献

Selective butyrate production from CO2 and methanol in microbial electrosynthesis - influence of pH 微生物电合成CO2和甲醇选择性丁酸盐——pH的影响

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-06 DOI: 10.1016/j.bioelechem.2025.109000

Hui Yao , Meritxell Romans-Casas , Igor Vassilev , Johanna M. Rinta-Kanto , Sebastià Puig , Antti J. Rissanen , Marika Kokko

{"title":"Selective butyrate production from CO2 and methanol in microbial electrosynthesis - influence of pH","authors":"Hui Yao , Meritxell Romans-Casas , Igor Vassilev , Johanna M. Rinta-Kanto , Sebastià Puig , Antti J. Rissanen , Marika Kokko","doi":"10.1016/j.bioelechem.2025.109000","DOIUrl":"10.1016/j.bioelechem.2025.109000","url":null,"abstract":"<div><div>Methanol assisted microbial electrosynthesis (MES) enables butyrate production from carbon dioxide and methanol using external electricity. However, the effects of operational parameters on butyrate formation remain unclear. By running three flat plate MES reactors with fed-batch mode at three controlled pH values (5.5, 6 and 7), the present study investigated the influence of pH on methanol assisted MES by comparing the process performance, microbial community structure, and genetic potential. The highest butyrate selectivity (87 % on carbon basis) and the highest butyrate production rate of 0.3 g L<sup>−1</sup> d<sup>−1</sup> were obtained at pH 6. At pH 7, a comparable butyrate production rate was achieved, yet with a lower selectivity (70 %) accompanied with acetate production. Butyrate production rate was considerably hindered at pH 5.5, reaching 0.1 g L<sup>−1</sup> d<sup>−1</sup>, while the selectivity reached was up to 81 %. Methanol and CO<sub>2</sub> consumption increased with pH, along with more negative cathodic potential and more negative redox potential. Furthermore, pH affected the thermodynamical feasibility of involved reactions. The results of metagenomic analyses suggest that <em>Eubacterium callanderi</em> dominated the microbial communities at all pH values, which was responsible for methanol and CO<sub>2</sub> assimilation via the Wood-Ljungdahl pathway and was likely the main butyrate producer via the reverse β-oxidation pathway.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 109000"},"PeriodicalIF":4.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical detection of superoxide anion in living systems: Recent trends and clinical implications 生命系统中超氧阴离子的电化学检测：最新趋势和临床意义

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-05 DOI: 10.1016/j.bioelechem.2025.108998

Caroline G. Sanz, Anca Aldea, Madalina M. Barsan

{"title":"Electrochemical detection of superoxide anion in living systems: Recent trends and clinical implications","authors":"Caroline G. Sanz, Anca Aldea, Madalina M. Barsan","doi":"10.1016/j.bioelechem.2025.108998","DOIUrl":"10.1016/j.bioelechem.2025.108998","url":null,"abstract":"<div><div>Superoxide plays a significant role in maintaining physiological states of living systems, with major roles in eradicating invading microorganisms and in cell signaling. It is regulated intricately by the enzyme superoxide dismutase (SOD), and when not properly regulated it can lead to cascade biological pathways with severe and irreversible damage to biofilms, tissue, and organs, being linked with many neurodegenerative diseases, atherosclerotic and cardiovascular diseases. Therefore, superoxide anion (<span><math><msubsup><mi>O</mi><mn>2</mn><mrow><mo>•</mo><mo>−</mo></mrow></msubsup></math></span>) detection has a tremendous potential in clinical diagnostics to assess oxidative stress in living cells. This comprehensive review aims to explore, discuss, and analyze recent trends in the electrochemical detection of <span><math><msubsup><mi>O</mi><mn>2</mn><mrow><mo>•</mo><mo>−</mo></mrow></msubsup></math></span> in living systems, focusing not only on the recognition mechanism for in vitro assays (living cell cultures/tissues) but also on the importance of the electrode design and operational parameters for in vivo measurements (implantable sensors). By analyzing current in vitro<em>/</em>in vivo electrochemical strategies we gather information that is helpful to overcome existing limitations in the dynamic monitoring of <span><math><msubsup><mi>O</mi><mn>2</mn><mrow><mo>•</mo><mo>−</mo></mrow></msubsup></math></span>, and further improve electrochemical strategies that can be adopted and applied to prevent its negative effect, with an insight into the pathophysiology of neurodegenerative disorders and even cellular malignancies that derive from its accumulation in living systems.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108998"},"PeriodicalIF":4.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving bleomycin electrochemotherapy with gold nanoparticles: first in vivo study on intra-tumoral field amplification 用金纳米颗粒改善博来霉素电疗：首次肿瘤内电场放大的体内研究

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-05 DOI: 10.1016/j.bioelechem.2025.108999

Eivina Radzevičiūtė-Valčiukė , Eglė Mickevičiūtė , Augustinas Želvys , Barbora Lekešytė , Paulina Malakauskaitė , Jovita Gečaitė , Veronika Malyško-Ptašinskė , Natalija German , Rokas Žalnėravičius , Vytautas Kašėta , Julita Kulbacka , Vitalij Novickij

{"title":"Improving bleomycin electrochemotherapy with gold nanoparticles: first in vivo study on intra-tumoral field amplification","authors":"Eivina Radzevičiūtė-Valčiukė , Eglė Mickevičiūtė , Augustinas Želvys , Barbora Lekešytė , Paulina Malakauskaitė , Jovita Gečaitė , Veronika Malyško-Ptašinskė , Natalija German , Rokas Žalnėravičius , Vytautas Kašėta , Julita Kulbacka , Vitalij Novickij","doi":"10.1016/j.bioelechem.2025.108999","DOIUrl":"10.1016/j.bioelechem.2025.108999","url":null,"abstract":"<div><div>Electrochemotherapy (ECT) is a cancer treatment approach that utilizes the application of electroporation (EP) with standard chemotherapeutic drugs, resulting in a locally enhanced chemotherapy effect due to enhanced intracellular drug delivery. The aim of this study was to demonstrate (for the first time) that microsecond-range bleomycin electrochemotherapy (1.5 kV/cm × 100 μs × 8 pulses, 1 Hz), when combined with gold nanoparticles (AuNPs, 13 and 46 nm), can be efficiently utilized for <em>in vivo</em> carcinoma tumor treatment. It was anticipated that AuNPs would promote a better treatment response due to local electric field amplification within the tumor as predicted by available <em>in vitro</em> research. We focus the attenuation of tumor progression and reduction of the frequency of metastasis incl. the immune response in the murine BALB/C and 4T1 cancer model. It is shown that the application of 13 nm AuNPs hardly influenced the dynamics of tumor progression (when compared to ECT alone), the synergistic effects are not statistically significant by the end of experiment, which is not the case <em>in vitro</em>. However, the application of 46 nm AuNPs significantly potentiated the efficacy of ECT, which confirms the promising alliance of conductive nanoparticles for local intra-tumoral electric field amplification and ECT.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108999"},"PeriodicalIF":4.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nature-powered bio-cathodes: Synergistic effects of laccase immobilization and green nanoparticles on enhanced PGEs for sustainable biofuel cells 自然驱动的生物阴极：漆酶固定化和绿色纳米颗粒对可持续生物燃料电池增强PGEs的协同效应

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-05-03 DOI: 10.1016/j.bioelechem.2025.108996

Shruthi Keerthi D. , M. Mukunda Vani , Likith G. , Sai Nikhil B. , Balaji Krishnamurthy

{"title":"Nature-powered bio-cathodes: Synergistic effects of laccase immobilization and green nanoparticles on enhanced PGEs for sustainable biofuel cells","authors":"Shruthi Keerthi D. , M. Mukunda Vani , Likith G. , Sai Nikhil B. , Balaji Krishnamurthy","doi":"10.1016/j.bioelechem.2025.108996","DOIUrl":"10.1016/j.bioelechem.2025.108996","url":null,"abstract":"<div><div>As part of growing energy demands and threats to ecology, there is an immense need for greener energy technologies that are novel, cost-effective, and ecofriendly. This paper presents a scalable strategy that uses biocompatible nanomaterials to modify the surface of a pencil graphite electrode (PGE) to enhance the performance of enzymatic biofuel cells (EBFC). <em>Rosa centifolia</em> flowers, an abundantly available plant extract, was used to green synthesize Ag and Cu nanoparticles and applied to various grades of pencil graphite lead surfaces (2H, HB, 2B) via dip-coating, and covalently immobilized with laccase enzyme. Unlike conventional nanoparticles, green-synthesized nanoparticles retain functional groups from phytochemicals that facilitate stable enzyme immobilization, resulting in effective electron transfer. Among the tested biocathodes 2B grade Lac/AgNP/PGE and Lac/CuNP/PGE demonstrated the highest open circuit potentials of 0.611 V and 0.498 V and current densities 1343.15 μA cm<sup>−2</sup> and 1054.17 μA cm<sup>−2</sup> respectively resulting in a significant raise of 70.84 % over pristine PGEs. Polarization studies revealed superior power density for Lac/AgNP/PGE-2B (20.629 μW cm<sup>−2</sup> at 65.21 μA cm<sup>−2</sup> current density) over Lac/CuNP/PGE-2B (17.39 μW cm<sup>−2</sup> at 61.9 μA cm<sup>−2</sup> current density). SEM confirmed enzyme immobilization, and FTIR and XPS validated the presence of carboxyl functional groups. Considering the stability of the modified electrode, Lac/AgNP/PGE-2B retained 62.93 % of its original current density on Day 20. This approach delivered a performance-enhanced biocathode design using functional nanomaterials and a bio-supportive strategy that displayed remarkable stability, longevity, and efficiency, and could potentially optimize the cost of EBFC design and promote miniaturization.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108996"},"PeriodicalIF":4.8,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-mode ratiometric electrochemical and turn-on fluorescence strategy for detecting biothiols in serum with all-in-one molecular probe 一键式分子探针检测血清中生物硫醇的双模式比例电化学和开启荧光策略

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-04-29 DOI: 10.1016/j.bioelechem.2025.108995

Hui Dong , Ke Xu , Zuying Wang , Wentian Chen , Yitong Jiang , Yidan Ye , Zhu Chang , Xu Zhu , Yanli Zhou , Maotian Xu , Yintang Zhang

{"title":"Two-mode ratiometric electrochemical and turn-on fluorescence strategy for detecting biothiols in serum with all-in-one molecular probe","authors":"Hui Dong , Ke Xu , Zuying Wang , Wentian Chen , Yitong Jiang , Yidan Ye , Zhu Chang , Xu Zhu , Yanli Zhou , Maotian Xu , Yintang Zhang","doi":"10.1016/j.bioelechem.2025.108995","DOIUrl":"10.1016/j.bioelechem.2025.108995","url":null,"abstract":"<div><div>Reactive sulfur species (RSS) play crucial roles in human physiology, with biothiols like hydrogen sulfide (H<sub>2</sub>S), cysteine (Cys), and glutathione (GSH) being key regulators of oxidative stress and signal transduction. Here, a dual-mode resorufin-based (Re) small-molecule probe (Re-RSS) with electrochemical and fluorescent properties was developed for biothiol detection. Using a ratiometric electrochemical strategy and a “turn-on” fluorescence method, Re-RSS enabled sensitive quantification of H<sub>2</sub>S, Cys, and GSH. The electrochemical approach showed linear ranges of 0–0.3 mM for H<sub>2</sub>S, 0–0.1 mM for Cys, and 0–0.08 mM for GSH. Fluorescence spectrometry revealed linear relationships in the ranges of 1 μM–0.3 mM (H<sub>2</sub>S), 2 μM–0.1 mM (Cys), and 5 μM–0.08 mM (GSH), with detection limits as low as 0.02, 0.01, and 0.08 μM (S/N = 3), respectively. This dual-mode sensor offers three major advantages: (1) dual-readout correction of system/background errors for improved accuracy; (2) independent signal transduction from distinct mechanisms, eliminating cross-interference; (3) negative-potential electrochemical detection free from biological interference. The direct dual-mode quantification highlights its significance and reliability for biothiol analysis in biological systems.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108995"},"PeriodicalIF":4.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The corrosion mechanism of Q355 steel electrically connected to the Al-Zn-In-Cd sacrificial anode: From microbial community to corrosion behavior analysis 电连接Al-Zn-In-Cd牺牲阳极的Q355钢腐蚀机理：从微生物群落到腐蚀行为分析

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-04-25 DOI: 10.1016/j.bioelechem.2025.108990

Lina Zhang , Fang Guan , Tianrong Zhan , Keliang Fan , Yugang Sang , Xiaodaong Zhao , Xiaofan Zhai , Jizhou Duan

{"title":"The corrosion mechanism of Q355 steel electrically connected to the Al-Zn-In-Cd sacrificial anode: From microbial community to corrosion behavior analysis","authors":"Lina Zhang , Fang Guan , Tianrong Zhan , Keliang Fan , Yugang Sang , Xiaodaong Zhao , Xiaofan Zhai , Jizhou Duan","doi":"10.1016/j.bioelechem.2025.108990","DOIUrl":"10.1016/j.bioelechem.2025.108990","url":null,"abstract":"<div><div>Microbially induced corrosion caused by sulfate-reducing bacteria (SRB) poses a significant threat to marine engineering facilities. Cathodic protection technology is a widely used method to prevent the corrosion of buried pipelines. The applied cathodic potential not only induce corrosion behavior change of steel but also triggers changes in the dominant microorganisms. In this study the corrosion behavior and microbial community characteristics of Q355 steel electrically connected to the Al-Zn-In-Cd sacrificial anode were studied. It was found that cathodic protection efficiency of Al-Zn-In-Cd alloy coupons with respect to Q355 steel in SRB media reached 40.17 %, while in natural seawater, the cathodic protection efficiency achieving a remarkable CP efficiency of 99.21 %. Besides of lepidocrocite (γ-FeOOH), halite, magnetite and Fe<sub>1-x</sub>S, more quartz was formed on cathodic protection protected steel surfaces compared with that without protection. Besides, more electroactive bacteria like <em>Exiguobacterium</em>, were found on the cathodic protection protected steel surfaces, which is related to the higher electron density and a polarized electric field.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108990"},"PeriodicalIF":4.8,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective targeting of E2F1 transcription factor via siRNA gene electrotransfer in HT-29 colorectal carcinoma xenografts 通过siRNA基因电转移在HT-29结直肠癌异种移植物中有效靶向E2F1转录因子

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-04-25 DOI: 10.1016/j.bioelechem.2025.108994

Tanja Jesenko , Simona Kranjc Brezar , Ziva Pisljar , Tim Bozic , Bostjan Markelc , Monica Cazzato , Gabriele Grassi , Maja Cemazar

{"title":"Effective targeting of E2F1 transcription factor via siRNA gene electrotransfer in HT-29 colorectal carcinoma xenografts","authors":"Tanja Jesenko , Simona Kranjc Brezar , Ziva Pisljar , Tim Bozic , Bostjan Markelc , Monica Cazzato , Gabriele Grassi , Maja Cemazar","doi":"10.1016/j.bioelechem.2025.108994","DOIUrl":"10.1016/j.bioelechem.2025.108994","url":null,"abstract":"<div><div>Colorectal cancer (CRC) remains a significant global health concern, with survival outcomes heavily dependent on the stage at diagnosis. Targeted therapies offer a promising approach to improve patient outcomes, particularly by addressing molecular drivers of tumor progression. One such target is the E2F1 transcription factor, a key regulator of the cell cycle and a contributor to proliferation, differentiation, apoptosis, metastasis, and chemoresistance in CRC. Previous studies have demonstrated the efficacy of E2F1 silencing via siRNA-loaded nanoliposomes in reducing tumor cell growth, but challenges such as immunogenicity and off-target effects have limited their in vivo application. In this study, we evaluated the potential of gene electrotransfer (GET) as a non-viral delivery system for delivery of therapeutic siRNA targeting E2F1 in the HT-29 CRC model. In vitro experiments showed effective silencing of E2F1 expression and a significant reduction in HT-29 cell survival. Subsequent in vivo studies confirmed the therapeutic potential of siE2F1 GET, with results demonstrating tumor growth delay, decreased proliferation, and increased necrosis in the tumors. This study establishes proof-of-principle for targeting E2F1 in CRC using GET, showcasing its versatility and therapeutic potential.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108994"},"PeriodicalIF":4.8,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-assembled nano-hybrid composite based on Cu/CuXO nanoflower decorated onto hBNNS for high-performance and ultra-sensitive electrochemical detection of CEA biomarker 基于hBNNS修饰的Cu/CuXO纳米花的自组装纳米杂化复合材料用于CEA生物标志物的高性能超灵敏电化学检测

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-04-24 DOI: 10.1016/j.bioelechem.2025.108993

Kanika Sharma , Nitin K. Puri , Bharti Singh

{"title":"Self-assembled nano-hybrid composite based on Cu/CuXO nanoflower decorated onto hBNNS for high-performance and ultra-sensitive electrochemical detection of CEA biomarker","authors":"Kanika Sharma , Nitin K. Puri , Bharti Singh","doi":"10.1016/j.bioelechem.2025.108993","DOIUrl":"10.1016/j.bioelechem.2025.108993","url":null,"abstract":"<div><div>Synergistic combination of metal/metal oxide with semiconductor as nano-hybrid composites (NHC), exhibits unmatched potential for developing nano-biosensors with superior stability, sensitivity, and selectivity. In this study, we report the fabrication of hydrothermally synthesized 3D<img>2D NHC based on self-assembled Cu/Cu<sub>X</sub>O-hBNNS for label-free detection of Carcinoembryonic Antigen (CEA). A systematic investigation into the synthesis of Cu<sub>X</sub>O-NF, hBNNS, and Cu/Cu<sub>X</sub>O-hBNNS NHC was carried out using extensive spectroscopic and advanced nanoscale imaging techniques. Uniform deposition of Cu/Cu<sub>X</sub>O-hBNNS films onto pre-hydrolyzed ITO electrodes was achieved at a low DC potential of 15 V using electrophoretic deposition (EPD). Optimum immunoelectrode efficacy was analyzed by monitoring antibody incubation time, electrolyte pH, and control study through FTIR and electrochemical techniques. Electrode study revealed remarkably improved surface chemistry upon Cu/Cu<sub>X</sub>O integration with hBNNS, yielding ∼74 % and ∼ 31 % increase in CV and DPV response along with 3-fold increase in diffusion coefficient compared to bare hBNNS. The sensing response of the BSA/Anti-CEA/Cu/Cu<sub>X</sub>O-hBNNS/ITO nano-biosensor detected CEA concentrations from 0 to 50 ng/mL utilizing [Fe(CN<sub>6</sub>)<sup>3−/4-</sup>] as a redox probe and demonstrated an exceptionally low limit of detection of 3.22 pg/mL (R<sup>2</sup> = 0.99998). Electrochemical clinical evaluation supported by ELISA test established that Cu/Cu<sub>X</sub>O-hBNNS-based nano-biosensor demonstrates exceptional shelf life, low cross-reactivity, and superior recovery rates in human serum, highlighting its effectiveness for precise and reliable detection.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108993"},"PeriodicalIF":4.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical analysis of abscisic acid based on cytochrome P450 707A3 基于细胞色素P450 707A3的脱落酸电化学分析

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-04-24 DOI: 10.1016/j.bioelechem.2025.108989

Yuling Cui , Nan Jiang , Yong Li , Yunhua Wu

{"title":"Electrochemical analysis of abscisic acid based on cytochrome P450 707A3","authors":"Yuling Cui , Nan Jiang , Yong Li , Yunhua Wu","doi":"10.1016/j.bioelechem.2025.108989","DOIUrl":"10.1016/j.bioelechem.2025.108989","url":null,"abstract":"<div><div>Cytochrome P450 707A3 (CYP707A3) from <em>Arabidopsis thaliana</em> is responsible for catalyzing hydroxylation of abscisic acid (ABA). In this study, the electrochemical analysis of ABA catalyzed by CYP707A3 protein were investigated. Direct electrochemical analysis of Fe<sup>3+</sup>/Fe<sup>2+</sup> redox peaks in CYP707A3 was performed at a pyrolytic graphite electrode with a redox potential of approximately −0.5 V in an oxygen-free phosphate-buffered solution (PBS, pH 7.0). Under aerobic conditions, with the addition of ABA, the electrode continuously supplied electrons to the iron porphyrin in CYP707A3 protein, resulting in a continuous increase in the reduction peak current. The relationship between the change in current and the concentration of ABA exhibited typical characteristics of the Michaelis-Menten kinetic mechanism, and the apparent Michaelis constant (K<sub>mapp</sub>) was calculated to be 77.08 nmol/L. The biosensor demonstrated a linear response to ABA within the range of 5 nM to 30 nM with a detection limit (LOD) of 4.85 nM (S/N = 3). The biosensor demonstrated high sensitivity, excellent reproducibility and good selectivity. It was applied to measure ABA content in the rice leaves under normal condition and drought stress, respectively.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108989"},"PeriodicalIF":4.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A highly sensitive MiRNA detection sensor powered by CRISPR/Cas13a and entropy-driven amplification 一种高灵敏度的MiRNA检测传感器，由CRISPR/Cas13a和熵驱动放大驱动

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-04-23 DOI: 10.1016/j.bioelechem.2025.108992

Qiang Tang , Qiujiao Liao , Xiaoling Huang , Hongran Huang , Qianli Tang , Kai Zhang , Xianjiu Liao

{"title":"A highly sensitive MiRNA detection sensor powered by CRISPR/Cas13a and entropy-driven amplification","authors":"Qiang Tang , Qiujiao Liao , Xiaoling Huang , Hongran Huang , Qianli Tang , Kai Zhang , Xianjiu Liao","doi":"10.1016/j.bioelechem.2025.108992","DOIUrl":"10.1016/j.bioelechem.2025.108992","url":null,"abstract":"<div><div>MicroRNAs (miRNAs) are critical regulators of numerous physiological and pathological processes, influencing gene expression and playing essential roles in cellular development, differentiation, and disease progression. Their sensitive and specific detection is vital for advancing biomedical research and clinical diagnostics, particularly for early disease detection and biomarker discovery. However, traditional miRNA detection methods often face significant challenges, such as limited sensitivity, insufficient specificity, and the inability to detect low-abundance miRNAs in complex biological samples. To overcome these limitations, we present a novel miRNA detection electrochemiluminescence (ECL) platform that integrates entropy-driven amplification with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a-mediated RNA cleavage. The entropy-driven amplification strategy exploits the thermodynamic advantages of nucleic acid hybridization, driving spontaneous molecular reorganization to amplify detection signals and achieve ultralow detection limits. CRISPR/Cas13a, an RNA-targeting nuclease, provides exceptional sequence specificity by recognizing and binding to target miRNA sequences, activating a collateral cleavage mechanism. This activity cleaves hairpin (HP) structure, generating a signal that further triggers EDA over DNA tetrahedron (DT) to induce a vigorous ECL response. Based on this strategy, we achieve rapid and precise quantification of miR-17 at femtomolar levels. Experimental results demonstrate high sensitivity, specificity, and the ability to analyze complex biological samples in the laboratory. This innovative approach holds great promise for advancing molecular diagnostics and personalized medicine.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108992"},"PeriodicalIF":4.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0