Bioelectrochemistry最新文献

Microalgae cells with extracellular gold nanoparticles for enhanced photobioelectrochemical activity 微藻细胞与细胞外金纳米粒子增强光生物电化学活性

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-10-01 Epub Date: 2026-03-04 DOI: 10.1016/j.bioelechem.2026.109264

Caio C.G. Silva , Alessandro Cacia , Hernán D. Rojas-Mantilla , Maria V.B. Zanoni , Saulo S. Garrido , Felipe Conzuelo

{"title":"Microalgae cells with extracellular gold nanoparticles for enhanced photobioelectrochemical activity","authors":"Caio C.G. Silva , Alessandro Cacia , Hernán D. Rojas-Mantilla , Maria V.B. Zanoni , Saulo S. Garrido , Felipe Conzuelo","doi":"10.1016/j.bioelechem.2026.109264","DOIUrl":"10.1016/j.bioelechem.2026.109264","url":null,"abstract":"<div><div>Photobioelectrochemical systems (PBEs) harness the machinery of photosynthetic microorganisms to convert solar energy into electricity. However, the inefficient electron transfer at the cell-electrode interface remains as the key performance limitation. Herein, we report a plasmonic biohybrid strategy to enhance extracellular electron transfer in <em>Chlorella</em>-based photobioelectrodes by integrating gold nanoparticles (AuNPs) localized on the microalgae membrane. Two approaches are investigated, consisting of physical mixing of isolated cells with AuNPs and cultivating microalgae in a growth medium supplemented with AuNPs, with the second approach allowing to obtain about significantly higher photocurrent responses. Optimized bioelectrodes yield photocurrent densities of up to 132 μA cm<sup>−2</sup>, 74% higher than bioelectrodes fabricated using unmodified cells. Spectral response analysis reveals a strong resonance at 525 nm, consistent with the plasmonic properties of AuNPs. Importantly, pigment content, growth kinetics, and membrane integrity are preserved, confirming the biocompatibility of the modification. This work presents a facile and effective route to engineer photosynthetic bioelectrodes using nanomaterials, advancing the design of high-performance PBEs for solar-to-electricity conversion.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"171 ","pages":"Article 109264"},"PeriodicalIF":4.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anthraquinone disulfonate as a stable redox mediator for efficient air-cathodes at neutral pH in dual-chamber microbial fuel cells 二磺酸蒽醌作为双室微生物燃料电池中性pH下高效空气阴极的氧化还原介质

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-10-01 Epub Date: 2026-03-09 DOI: 10.1016/j.bioelechem.2026.109279

Antoine Vautier, James A. Behan, Charlotte Bodin, Florence Geneste, Frédéric Barrière

{"title":"Anthraquinone disulfonate as a stable redox mediator for efficient air-cathodes at neutral pH in dual-chamber microbial fuel cells","authors":"Antoine Vautier, James A. Behan, Charlotte Bodin, Florence Geneste, Frédéric Barrière","doi":"10.1016/j.bioelechem.2026.109279","DOIUrl":"10.1016/j.bioelechem.2026.109279","url":null,"abstract":"<div><div>Microbial Fuel Cells (MFCs) are commonly developed as organic-matter oxidizing bioanodes with abiotic air cathodes. However, O<sub>2</sub> reduction requires active aeration and/or the use of expensive catalysts using noble metals. In this study, 2,7-anthraquinone disulfonate (2,7-AQDS), an organic redox mediator commonly used in aqueous redox flow battery systems (AORFBs), served as a redox-stable intermediate for oxygen reduction. Dual-chamber MFC pilots were developed with 2,7-AQDS in the catholyte under both anoxic and aerobic conditions and compared to pilots with ferricyanide catholytes. In both conditions, cyclic voltammetry studies confirmed similar and efficient electroactivity despite the proximity AQDS formal redox potential to that of acetate oxidation. Mediated air-cathodes achieved open-circuit voltage (OCV) of 510 mV and current densities of 140 μA/cm<sup>2</sup>, nearly double those of air-only cathodes (72 μA/cm<sup>2</sup>), while delivering a 33% higher power density (12 mW/m<sup>2</sup> vs. 8 mW/m<sup>2</sup>). Passive catholyte aeration enabled continuous reoxidation of reduced 2,7-AQDS at 8.8 × 10<sup>−8</sup> mol/s, exceeding the AQDS reduction rate by the bioanode (2.5 × 10<sup>−10</sup> mol/s), thus ensuring effective self-regeneration and stable AQDS concentration. These results demonstrate that AQDS coupled with passive oxygen supply sustains biofilm activity with enhances current and power, and allow long-term / low-maintenance MFC operation and organic-matter oxidation.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"171 ","pages":"Article 109279"},"PeriodicalIF":4.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing fast scan cyclic voltammetry at carbon fiber microelectrodes to quantify short chain fatty acids in situ 碳纤维微电极快速扫描循环伏安法原位定量短链脂肪酸。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-10-01 Epub Date: 2026-03-04 DOI: 10.1016/j.bioelechem.2026.109266

Terdha Narla , Uma Nudurupati, Yangguang Ou

{"title":"Developing fast scan cyclic voltammetry at carbon fiber microelectrodes to quantify short chain fatty acids in situ","authors":"Terdha Narla , Uma Nudurupati, Yangguang Ou","doi":"10.1016/j.bioelechem.2026.109266","DOIUrl":"10.1016/j.bioelechem.2026.109266","url":null,"abstract":"<div><div>Acetic, propionic, and butyric acids are short chain fatty acids (SCFAs) and the most abundant metabolites produced by gut bacteria. To uncover mechanistic insights of their function throughout the body, it is critical to measure rapid SCFA fluxes in sites of action such as brain, muscle, and skin. Current approaches have focused on fecal and plasma measurements, where SCFA levels are low and not representative of tissue-specific concentrations or fluxes. Thus, a rapid, <em>in situ</em> methodology is needed. Fast scan cyclic voltammetry (FSCV) at carbon fiber microelectrodes (CFMs) has the spatiotemporal resolution to fill this need. However, the electrochemical response of CFMs to SCFAs have not been explored until now. By coupling FSCV at CFMs with flow injection analysis of each SCFA, we demonstrate there are distinct peaks in the cyclic voltammograms for each fatty acid. Some of these peaks display faradaic behavior in scan rate, holding potential, and switching potential experiments. Interestingly, we identify several peaks that are concentration-sensitive and therefore are promising quantifiable markers of SCFA dynamics and fluxes. This work lays the foundation in understanding the response of CFMs to fatty acids and demonstrate the utility of FSCV at CFMs for the <em>in situ</em> quantitation of SCFAs.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"171 ","pages":"Article 109266"},"PeriodicalIF":4.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147371938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotip acetylcholine biosensor reveals cholinergic differentiated SH-SY5Y cells release partial vesicle content during exocytosis 纳米尖端乙酰胆碱生物传感器显示胆碱能分化的SH-SY5Y细胞在胞吐过程中释放部分囊泡内容物

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-10-01 Epub Date: 2026-02-26 DOI: 10.1016/j.bioelechem.2026.109260

Yuanmo Wang , Ajay Pradhan , Pankaj Gupta , Jörg Hanrieder , Henrik Zetterberg , Ann-Sofie Cans

{"title":"Nanotip acetylcholine biosensor reveals cholinergic differentiated SH-SY5Y cells release partial vesicle content during exocytosis","authors":"Yuanmo Wang , Ajay Pradhan , Pankaj Gupta , Jörg Hanrieder , Henrik Zetterberg , Ann-Sofie Cans","doi":"10.1016/j.bioelechem.2026.109260","DOIUrl":"10.1016/j.bioelechem.2026.109260","url":null,"abstract":"<div><div>Acetylcholine (ACh) is a central neurotransmitter in cognitive function, motor control, and synaptic modulation, yet its electrochemical inactivity and the rapid kinetics of exocytosis have hindered real-time quantal measurements. Micrometer-scale enzymatic ACh biosensors previously enabled sub-millisecond extracellular recordings but were too large for synaptic positioning and intracellular recordings. Here we present a short, ultrafast and low-noise amperometric ACh biosensor based on a needle-shaped carbon fiber nanotip electrode functionalized with gold nanoparticles and enzymes. The miniaturized geometry allows precise placement at neurite release sites and minimally invasive insertion into the cell cytoplasm, enabling high-temporal resolution monitoring of presynaptic exocytosis together with quantification of intracellular ACh vesicle content. We applied this platform to differentiated human cholinergic SH-SY5Y neuroblastoma cells, an established yet underutilized cell model for cholinergic signaling. The nanotip sensor successfully captured amperometric spikes from both intracellular vesicle burst events and presynaptic ACh release. Intracellular events released a larger amount of ACh than presynaptic exocytosis events, indicating a predominance of partial exocytosis mode in these cells. These results demonstrate the nanotip ACh biosensor as a unique tool for probing fusion pore dynamics at subcellular resolution and for providing quantitative insight into the quantal nature of cholinergic signaling in human neuronal models.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"171 ","pages":"Article 109260"},"PeriodicalIF":4.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemistry of redox enzymes: from functional enzyme immobilization to enzymatic bio-electrochemical devices (tutorial) 氧化还原酶的电化学：从功能酶固定化到酶生物电化学装置（教程）

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-10-01 Epub Date: 2026-03-04 DOI: 10.1016/j.bioelechem.2026.109265

A. Guessab, I. Mazurenko, E. Lojou, A. de Poulpiquet

{"title":"Electrochemistry of redox enzymes: from functional enzyme immobilization to enzymatic bio-electrochemical devices (tutorial)","authors":"A. Guessab, I. Mazurenko, E. Lojou, A. de Poulpiquet","doi":"10.1016/j.bioelechem.2026.109265","DOIUrl":"10.1016/j.bioelechem.2026.109265","url":null,"abstract":"<div><div>Electrochemistry of redox enzymes is a multidisciplinary field, and students who join labs specialized in bio-electrochemistry have various backgrounds: chemistry, biology, biotechnologies, nanotechnologies, physics, etc., so that their level in the involved disciplines is often heterogeneous, and they sometimes lack the basic knowledge about either of the fields concerned by their project (physical chemistry, electrochemistry, enzymology, etc.). Projects that gather experienced researchers from these different areas also sometimes suffer from a lack of understanding between the partners. This tutorial, which follows a lecture given at the first winter school of the French group of bio-electrochemistry (GFB), aims at guiding newcomers in the field and provides advice for more in-depth and specialized literature. It seeks to provide a solid theoretical and experimental foundation. Redox enzymes and the basic methods of immobilization at the electrode are introduced. The mechanisms of direct and mediated electron transfers are explained. An important electrochemical method, cyclic voltammetry, is explained, and the article describes how to extract information about enzyme/electrode and catalytic reactions. Finally, the tutorial presents two enzymatic bio-electrochemical devices, enzymatic fuel cells and enzymatic biosensors, and provides a guide for their electrochemical characterization.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"171 ","pages":"Article 109265"},"PeriodicalIF":4.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proof-of-concept bioelectrochemical characterization of Mycolicibacterium smegmatis for diagnostic applications 耻垢分枝杆菌诊断应用的概念验证生物电化学表征

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-10-01 Epub Date: 2026-03-09 DOI: 10.1016/j.bioelechem.2026.109277

Sunday Olakunle Oguntomi , Abhipsa Sahu , Yili Yang , Enrico Marsili

{"title":"Proof-of-concept bioelectrochemical characterization of Mycolicibacterium smegmatis for diagnostic applications","authors":"Sunday Olakunle Oguntomi , Abhipsa Sahu , Yili Yang , Enrico Marsili","doi":"10.1016/j.bioelechem.2026.109277","DOIUrl":"10.1016/j.bioelechem.2026.109277","url":null,"abstract":"<div><div>Elevated urease activity in tuberculosis-infected individuals is often suggestive of bacterial virulence by promoting survivability and establishment of the causative pathogen (<em>Mycobacterium tuberculosis</em>) in the host. Thus, heightened urease activity could be a promising biomarker for screening pathogenic mycobacterial strains<em>.</em> In this proof-of-concept study, we evaluate the suitability of electrochemical techniques for direct screening of mycobacterial strains based on urease activity. <em>Mycolicibacterium smegmatis</em> wildtype (WT), a derived knockout mutant of the LCP protein family (<em>∆0107</em>) with high urease activity, and its complement strain (<em>c-0107</em>) were coated in polydopamine (PDA) and immobilized on a screen-printed electrode (SPE) for microscopy and electrochemical characterization, in presence of exogenous redox mediators: potassium ferricyanide, anthraquinone, and riboflavin. Microscopy showed compatibility of PDA as a coating material for mycobacterial cells. Electrochemical analysis results indicated enhanced <em>M. smegmatis</em> electroactivity in the presence of anthraquinone, with higher current output in the strain with high urease activity. Experiments at different urea concentrations suggest that urea is not required for this screening approach. This work shows that electrochemical data can potentially distinguish mycobacterial strains based on urease activity. It also lays a groundwork in the model mycobacteria <em>M. smegmatis</em>, which could facilitate development of assays for pathogenic mycobacterial species.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"171 ","pages":"Article 109277"},"PeriodicalIF":4.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147388331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of a MWCNTs-AuNPs composite-modified electrochemical immunosensor with anti-fouling property for highly sensitive and rapid detection of BNP in myocardial infarction-induced heart failure diagnosis MWCNTs-AuNPs复合修饰抗污染电化学免疫传感器在心肌梗死性心力衰竭诊断中的高灵敏度和快速检测

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-10-01 Epub Date: 2026-02-28 DOI: 10.1016/j.bioelechem.2026.109258

Jiaru Fu , Dewei Huang , Bolu Sun , Chengwen Li , Yuqiong Wu , Huajie Li , Jiali Kang , Haiying He , Xiangdong Wang , Yaru Zhang , Xuanxiu Da , Mian Chen , Lin Yang

{"title":"Optimization of a MWCNTs-AuNPs composite-modified electrochemical immunosensor with anti-fouling property for highly sensitive and rapid detection of BNP in myocardial infarction-induced heart failure diagnosis","authors":"Jiaru Fu , Dewei Huang , Bolu Sun , Chengwen Li , Yuqiong Wu , Huajie Li , Jiali Kang , Haiying He , Xiangdong Wang , Yaru Zhang , Xuanxiu Da , Mian Chen , Lin Yang","doi":"10.1016/j.bioelechem.2026.109258","DOIUrl":"10.1016/j.bioelechem.2026.109258","url":null,"abstract":"<div><div>Brain natriuretic peptide (BNP) is a sensitive biomarker for acute heart failure (AHF). Rapid, sensitive, and anti-fouling BNP detection in serum remains clinically unmet. Herein, a novel electrochemical immunosensor with synergistic signal amplification and anti-fouling ability was constructed via layered AuNPs/MWCNTs-AuNPs modification. Bottom AuNPs mitigate MWCNTs agglomeration and enable stable Anti-BNP immobilization via Au<img>S bonds, MWCNTs enhance conductivity, and top AuNPs amplify signals, forming an efficient electron-transport network. A nitrocellulose (NC) membrane was laminated for anti-fouling via rapid contaminant sieving. Under optimization, the sensor shows a linear range of 7.81–8 × 10<sup>3</sup> pg/mL for BNP, with a detection limit of 8.14 × 10<sup>−2</sup> pg/mL (3σ, <em>n</em> = 11), outperforming ELISA. It exhibits excellent selectivity (vs. cTn-I, glucose, IL-6), repeatability (RSD = 2.3%), and stability (92.4% retention over 30 days). Serum recovery rates of 102–105% confirm anti-fouling reliability. This cost-effective platform promises POCT for AHF and underpins anti-fouling biosensor development.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"171 ","pages":"Article 109258"},"PeriodicalIF":4.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147363735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioluminescence-based electrochemical sensor for dual-mode direct hydrocarbon detection in saline water utilizing Photobacterium leiognathi and d-luciferin-modified au-SPE 利用光杆菌和d-荧光素修饰的au-SPE进行盐水中碳氢化合物双模直接检测的生物发光电化学传感器

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-08-01 Epub Date: 2026-01-24 DOI: 10.1016/j.bioelechem.2026.109233

Arash Rasti , Muhamad Afiq Aziz , Zaira Zaman Chowdhury , Sook Mei Khor

{"title":"Bioluminescence-based electrochemical sensor for dual-mode direct hydrocarbon detection in saline water utilizing Photobacterium leiognathi and d-luciferin-modified au-SPE","authors":"Arash Rasti , Muhamad Afiq Aziz , Zaira Zaman Chowdhury , Sook Mei Khor","doi":"10.1016/j.bioelechem.2026.109233","DOIUrl":"10.1016/j.bioelechem.2026.109233","url":null,"abstract":"<div><div>Early detection of hydrocarbon pollution in saline ecosystems faces challenges due to their complexity and the limitations of current detection methods. Salinity, temperature variations, the presence of other organic matter, and weathering processes hinder the effectiveness of traditional techniques, while the cost and complexity of some advanced detection technologies limit their widespread application. In this study, a gold screen-printed electrode (Au-SPE) modified with d-luciferin was developed to monitor the metabolic response of <em>Photobacterium leiognathi</em> to hexane and aromatic hydrocarbons in saline water and seawater. The sensor works by capturing adenosine triphosphate (ATP)-dependent electron release associated with bacterial bioluminescence, which varies according to the type of hydrocarbon. D-luciferin was immobilized on the Au-SPE via a self-assembled monolayer using ethylenediamine and EDC/NHS coupling to create a biocompatible interface. CV analysis revealed time-dependent shifts of anodic and cathodic peaks from −0.5 V to +0.5 V. The presence of aromatic hydrocarbons increased both bioluminescence light emission and current, indicating metabolic stimulation. In contrast, hexane suppressed bioluminescence and decreased current, indicating metabolic inhibition. These distinct responses enable rapid and selective differentiation between different types of hydrocarbons. The developed biosensor exhibits strong potential for real-time monitoring of oil contamination and assessing water quality in saline ecosystems.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"170 ","pages":"Article 109233"},"PeriodicalIF":4.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resourceful utilization of Bougainvillea horticultural waste for synchronous degradation and power generation in MFCs 九重葛园艺废弃物在mfc同步降解和发电中的资源化利用

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-08-01 Epub Date: 2026-01-24 DOI: 10.1016/j.bioelechem.2026.109237

Yeling Zhou, Yicheng Wu, Bingjun Shu, Feipeng Xu

{"title":"Resourceful utilization of Bougainvillea horticultural waste for synchronous degradation and power generation in MFCs","authors":"Yeling Zhou, Yicheng Wu, Bingjun Shu, Feipeng Xu","doi":"10.1016/j.bioelechem.2026.109237","DOIUrl":"10.1016/j.bioelechem.2026.109237","url":null,"abstract":"<div><div>The disposal of horticultural waste derived from <em>Bougainvillea</em> species poses substantial environmental challenges in coastal cities of southeastern China. This study innovatively employs <em>Bougainvillea</em> horticultural waste (BHW) as the main substrate in microbial fuel cells (MFCs) for simultaneous organic degradation and bioenergy recovery, with an acid-pretreated BHW-fed MFC (MFC-ABG) included for comparison. Results indicated that the untreated BHW-fed MFC (MFC-BG) achieved a peak voltage of 0.401 V and sustained operation for 19 days, coupled with 78.7% polysaccharide removal. Metagenomics showed that MFC-BG significantly enriched electroactive <em>Geobacter</em> (29.39%) and hydrolytic <em>Proteiniphilum</em> (2.69%), driving lignocellulose decomposition through oxidative auxiliary enzymes (AA4/AA6). Comparatively, MFC-ABG achieved an enhanced voltage of 0.706 V and a high polysaccharide reduction efficiency of 85.6%, benefits attributable to acid-induced substrate solubilization and glycoside hydrolase (GH)-dominated enzymatic shifts. Although microbial community diversity declined in both MFC systems, MFC-BG retained a higher species richness (MFC-BG: Sobs = 28,209; MFC-ABG: Sobs = 25,746), reflecting the adaptive resilience of the associated microbial community. This study confirms BHW as a viable feedstock for MFCs and clarifies the microbial mechanisms underlying the synergistic coupling of substrate degradation and electron transfer</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"170 ","pages":"Article 109237"},"PeriodicalIF":4.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the redox signature and reactivity of wines with differential pulse voltammetry 用差分脉冲伏安法破译葡萄酒的氧化还原特征和反应性。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-08-01 Epub Date: 2026-02-03 DOI: 10.1016/j.bioelechem.2026.109239

Alice L. Dauphin, Samuel Guilbault, Stéphane Arbault

{"title":"Deciphering the redox signature and reactivity of wines with differential pulse voltammetry","authors":"Alice L. Dauphin, Samuel Guilbault, Stéphane Arbault","doi":"10.1016/j.bioelechem.2026.109239","DOIUrl":"10.1016/j.bioelechem.2026.109239","url":null,"abstract":"<div><div>Phenolic compounds, including polyphenols and tannins, contribute to the sensory characteristics of wines and help protect them against oxidation through their reductive properties. Linear or cyclic voltammetry methods were previously reported to monitor specific wine phenolic compounds and decipher on their antioxidant activity. Pulsed voltammetry methods improve selectivity and accuracy and recently raised further interest for wine studies. We report the use of differential pulse voltammetry-DPV to characterize each wine redox profile and reactivity. Without any prior solution preparation, DPV analysis in wine provides curves displaying several oxidation peaks assigned to families of reductive phenolic acids or anthocyanins, flavonoids and tannins. Wine redox profiles vary as a function of their color, winemaking process, grape variety, vintage, etc. DPV and cyclic voltammetry-CV allowed further to study wines when changing their composition in caffeic and gallic acids, demonstrating the reactivity between phenolic species. Finally, the oxidation of a red wine under air and oxygen-saturated conditions was monitored by colorimetric and DPV analyses, directly showing the correlation between color browning, decrease of reductive ability and dissolved oxygen level. This work demonstrates the effectiveness of DPV in directly deciphering the oxidation-reduction processes occurring during winemaking and wine ageing.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"170 ","pages":"Article 109239"},"PeriodicalIF":4.5,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146136935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0