Bioelectrochemistry最新文献

Hybridization chain reaction and CRISPR/Cas12a-integrated biosensor for precise Ago2 detection

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-26 DOI: 10.1016/j.bioelechem.2025.108975

Qiang Tang , Jiayi Zhang , Jialuo Pang , Yingying Huang , Ying Guan , Yuanxun Gong , Qianli Tang , Kai Zhang , Xianjiu Liao

{"title":"Hybridization chain reaction and CRISPR/Cas12a-integrated biosensor for precise Ago2 detection","authors":"Qiang Tang , Jiayi Zhang , Jialuo Pang , Yingying Huang , Ying Guan , Yuanxun Gong , Qianli Tang , Kai Zhang , Xianjiu Liao","doi":"10.1016/j.bioelechem.2025.108975","DOIUrl":"10.1016/j.bioelechem.2025.108975","url":null,"abstract":"<div><div>This study introduces an innovative electrochemiluminescence (ECL) biosensor for the highly sensitive and specific detection of Argonaute 2 (Ago2) activity. Ago2, a key enzyme in the RNA interference (RNAi) pathway, plays a crucial role in gene regulation, and its dysregulation is associated with diseases such as cancer and viral infections. The biosensor integrates hybridization chain reaction (HCR) amplification and the CRISPR/Cas12a system, leveraging a multi-stage signal amplification strategy. The detection mechanism begins with Ago2-mediated cleavage of a designed hairpin RNA (HP-RNA), releasing single-stranded RNA (ssRNA) that triggers HCR. This amplification step generates long DNA polymers, which serve as activators for the CRISPR/Cas12a system. Cas12a's collateral cleavage activity amplifies the signal further by cleaving a DNA reporter labeled with a ruthenium-based luminophore, enhancing the ECL output. This dual amplification strategy achieves exceptional sensitivity, with a detection limit of 0.126 aM. The biosensor demonstrates excellent specificity, distinguishing Ago2 from other Argonaute proteins, and maintains high reproducibility and stability, retaining 94 % of its signal after two weeks of storage. Real-world applicability was confirmed by accurately detecting Ago2 in spiked cell lysates, with recovery rates exceeding 100 %. The combination of HCR, CRISPR/Cas12a, and ECL establishes a robust platform for biomarker detection, offering superior sensitivity and adaptability for clinical diagnostics, disease monitoring, and therapeutic evaluation. This biosensor represents a significant advancement in the development of next-generation diagnostic tools.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108975"},"PeriodicalIF":4.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A high-sensitivity label-free electrochemical aptasensor for point-of-care measurements of low-density lipoprotein in plasma based on aptamer and MXene-CMCS-Hemin nanocomposites

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-20 DOI: 10.1016/j.bioelechem.2025.108972

Jintao Liang , Shuaikang Dong , Xiaohong Tan , Junyan Gong , Xuyang Chen , Zhide Zhou , Guiyin Li

{"title":"A high-sensitivity label-free electrochemical aptasensor for point-of-care measurements of low-density lipoprotein in plasma based on aptamer and MXene-CMCS-Hemin nanocomposites","authors":"Jintao Liang , Shuaikang Dong , Xiaohong Tan , Junyan Gong , Xuyang Chen , Zhide Zhou , Guiyin Li","doi":"10.1016/j.bioelechem.2025.108972","DOIUrl":"10.1016/j.bioelechem.2025.108972","url":null,"abstract":"<div><div>Cardiovascular disease (CVD) remains a significant worldwide health challenge, with mortality rates rising rapidly. Low-density lipoprotein (LDL) is a crucial serum biomarker for the early diagnosis of CVD, which can significantly improve outcomes and reduce mortality. Herein, a label-free electrochemical aptasensor for rapid detection of LDL was developed based on the titanium carbide–carboxymethyl chitosan–hemin (MXene-CMCS-Hemin) nanocomposites as the electrochemical signal probe. Firstly, gold nanoparticles (Au NPs) were electrodeposited onto a screen-printed carbon electrode (SPCE) to form a conductive substrate. Subsequently, the MXene-CMCS-Hemin nanocomposites were anchored onto the Au NPs/SPCE surface. Then LDL<sub>Apt</sub> was immobilized on the surface of MXene-CMCS-Hemin/Au NPs/SPCE to construct the electrochemical aptasensor. When LDL specifically bound with the LDL<sub>Apt</sub> to form LDL-LDL<sub>Apt</sub> complexes, hindering the electron transfer and reducing the Hemin oxidation current, LDL detection can be achieved via differential pulse voltammetry (DPV). Under optimal circumstances, the changes of Hemin's oxidation current showed a good linear response with LDL concentration in the range of 0.1–4.0 μmol/L with a detection limit of 0.095 μmol/L (S/N = 3). The aptasensor demonstrated good performance with the relative errors of 0.60 % to 6.58 % for the direct detection of LDL in human serum samples, which offers a novel tool for the clinical diagnosis of CVD.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108972"},"PeriodicalIF":4.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143696806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The unusual formaldehyde-induced activation of [NiFe]-hydrogenase: Implications from protein film electrochemistry and infrared spectroscopy

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-19 DOI: 10.1016/j.bioelechem.2025.108974

Lei Wan , Yanxin Gao , Serena DeBeer , Olaf Rüdiger

{"title":"The unusual formaldehyde-induced activation of [NiFe]-hydrogenase: Implications from protein film electrochemistry and infrared spectroscopy","authors":"Lei Wan , Yanxin Gao , Serena DeBeer , Olaf Rüdiger","doi":"10.1016/j.bioelechem.2025.108974","DOIUrl":"10.1016/j.bioelechem.2025.108974","url":null,"abstract":"<div><div>Here we investigate how formaldehyde (HCHO), a known strong inhibitor of [FeFe]‑hydrogenases and a mild inhibitor of [NiFe]‑hydrogenases, may exert more complex effects on this group of metalloenzymes, which reversibly catalyze the 2H<sup>+</sup>/H₂ reaction. We investigated the [NiFe]‑hydrogenase Hyd-2 from <em>E. coli</em> using protein film electrochemistry, a technique that enables the measurement of enzyme activity when the enzyme is adequately adsorbed on the electrode. The effect of HCHO on the electrocatalytic performance of Hyd-2 is highly dependent on the buffer pH and the direction of catalysis. During H₂ production, HCHO consistently acts as an inhibitor of Hyd-2. However, this effect is reversed in acidic pH values, where HCHO can mildly enhance the electrocatalytic H₂ oxidation by Hyd-2. FTIR investigations did not detect any new redox intermediate resulting from the inhibition or activation. Therefore, we propose that HCHO - a natural electrophile that can readily react with nucleophiles and proton acceptors - may facilitate the transfer protons during the rapid transformation of different redox species participating in the catalytic cycle of [NiFe]‑hydrogenases.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108974"},"PeriodicalIF":4.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural bioelectrochemistry of direct electron transfer-type multimeric dehydrogenases: Basic principle and rational strategies

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-17 DOI: 10.1016/j.bioelechem.2025.108973

Konatsu Ichikawa, Taiki Adachi, Keisei Sowa

引用次数: 0

Study on effect of electroporation combining high- and low-frequency harmonics

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-17 DOI: 10.1016/j.bioelechem.2025.108971

Borja López-Alonso , Tamara Polajžer , Matej Reberšek , Héctor Sarnago , Óscar Lucía , Damijan Miklavčič

{"title":"Study on effect of electroporation combining high- and low-frequency harmonics","authors":"Borja López-Alonso , Tamara Polajžer , Matej Reberšek , Héctor Sarnago , Óscar Lucía , Damijan Miklavčič","doi":"10.1016/j.bioelechem.2025.108971","DOIUrl":"10.1016/j.bioelechem.2025.108971","url":null,"abstract":"<div><div>The effects of electroporation are highly influenced by the shape of the applied waveform. This waveform shape can modify the transmitted energy and current flow patterns, impacting the electric field distribution, temperature rise among others. These interactions, along with their synergies with electroporation, are being explored across various industrial and research domains. For instance, in the biomedical field, high-frequency waveforms such as nanosecond pulses offer distinct advantages, while in the food industry, controlled temperature increases combined with electroporation are beneficial. However, in the medical field, the effects of combining high-frequency waveforms (in the MHz range) with low-frequency waveforms (in the kHz range commonly used in clinical electroporation) have not been thoroughly studied, though hypotheses have been proposed regarding their potential effects.</div><div>In this paper, proof of concept of the effect of the combination of two harmonics is presented using three different strategies to investigate new electroporation protocols. To support this study, a specialized electrical and thermal test bench was developed to control and evaluate the feasibility and potential of possible synergy between high- and low-frequency waveforms to electroporation using an <em>in vitro</em> model.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108971"},"PeriodicalIF":4.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrochemical biosensor using AuNPs-Ti3C2Tx and ARGET ATRP reactions as signal amplification strategies for ultra-sensitive detection of HER2 protein

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-15 DOI: 10.1016/j.bioelechem.2025.108970

Yanju Liu , Aozi Feng , Fengzhi Li , Liying Zhao , Qiyong Cai , Daoxiang Li , HuaixiaYang

{"title":"An electrochemical biosensor using AuNPs-Ti3C2Tx and ARGET ATRP reactions as signal amplification strategies for ultra-sensitive detection of HER2 protein","authors":"Yanju Liu , Aozi Feng , Fengzhi Li , Liying Zhao , Qiyong Cai , Daoxiang Li , HuaixiaYang","doi":"10.1016/j.bioelechem.2025.108970","DOIUrl":"10.1016/j.bioelechem.2025.108970","url":null,"abstract":"<div><div>Human epidermal growth factor receptor 2 (HER2) status is an important factor in evaluating the prognosis of breast cancer patients. Therefore, it is particularly important to develop a simple and sensitive method for the detection of HER2-positive breast cancer. Here, an ultra-sensitive electrochemical biosensor for detecting HER2-specific proteins was assembled using gold nanoparticles and Two-dimensional carbides (AuNPs-Ti<sub>3</sub>C<sub>2</sub>Tx) as a conducting substrate. The prepared AuNPs-Ti<sub>3</sub>C<sub>2</sub>Tx not only has good electrical conductivity and strong electrochemical signal output, but also provides a large number of active sites for the Au<img>S bonds assembly aptamer. In addition, the antibodies-modified functionalized graphene oxide (GO) as a carrier platform, which provides an additional boost for the detection of trace targets with high sensitivity under optimal conditions. Afterwards,HER2 protein was detected by signal amplification effect of AuNPs-Ti<sub>3</sub>C<sub>2</sub>Tx and functionalized GO combined with Electron transfer activated regeneration catalyst atomic transfer radical polymerization (ARGET ATRP). In the range of 1 to 10<sup>5</sup> ng·mL<sup>−1</sup>, there was a good linear relationship between the HER2 concentration and the signal intensity, with a limit of detection of 0.19 pg·mL<sup>−1</sup>. Moreover, this method has good selectivity and stability, and then still maintains good detection performance and strong anti-interference ability in the complex environment of normal human serum, which is expected to be applied in clinical application.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108970"},"PeriodicalIF":4.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143681017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Jasplakinolide delivery in rhabdomyosarcoma cells using pulsed electric fields (PEFs) for enhanced therapeutic impact

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-12 DOI: 10.1016/j.bioelechem.2025.108969

Anna Szewczyk , Nina Rembiałkowska , Marta Migocka-Patrzałek , Wojciech Szlasa , Agnieszka Chwiłkowska , Małgorzata Daczewska , Vitalij Novickij , Julita Kulbacka

{"title":"Optimizing Jasplakinolide delivery in rhabdomyosarcoma cells using pulsed electric fields (PEFs) for enhanced therapeutic impact","authors":"Anna Szewczyk , Nina Rembiałkowska , Marta Migocka-Patrzałek , Wojciech Szlasa , Agnieszka Chwiłkowska , Małgorzata Daczewska , Vitalij Novickij , Julita Kulbacka","doi":"10.1016/j.bioelechem.2025.108969","DOIUrl":"10.1016/j.bioelechem.2025.108969","url":null,"abstract":"<div><div>This study explores the combination of jasplakinolide with electroporation (JSP + EP), a method enhancing targeted molecule delivery. CHO-K1 (Chinese hamster ovarian), C2C12 (mouse myoblast), and RD (rhabdomyosarcoma) cells were treated with jasplakinolide (50 nM) in HEPES buffer and exposed to electrical pulses (0.8–1.2 kV/cm). Cell viability was measured via the MTS assay, cytoskeleton structure was assessed with confocal microscopy, and docking studies examined jasplakinolide-actin interactions. The combination of jasplakinolide and electric pulses synergistically affected RMS cells (Rhabdomyosarcoma), causing significant cytoskeletal changes and reduced viability. Docking studies revealed that jasplakinolide interacts with both monomeric and filamentous actin, highlighting a dual mechanism. Confocal imaging showed substantial actin cytoskeleton disruption in cancer cells, with minimal effects on normal cells. Jasplakinolide combined with electric pulses can specifically target cancer cells with less cytotoxicity to normal cells, potentially reducing side effects following the clinical procedure.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108969"},"PeriodicalIF":4.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acceleration of microbiologically influenced corrosion of 304 stainless steel caused by photolysis of riboflavin

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-08 DOI: 10.1016/j.bioelechem.2025.108968

Hao Zhang , Zhongyu Wu , Yuntian Lou , Weiwei Chang , Jingzhi Yang , Hongchang Qian , Dawei Zhang

{"title":"Acceleration of microbiologically influenced corrosion of 304 stainless steel caused by photolysis of riboflavin","authors":"Hao Zhang , Zhongyu Wu , Yuntian Lou , Weiwei Chang , Jingzhi Yang , Hongchang Qian , Dawei Zhang","doi":"10.1016/j.bioelechem.2025.108968","DOIUrl":"10.1016/j.bioelechem.2025.108968","url":null,"abstract":"<div><div>This study investigates the effect of riboflavin on microbiologically influenced corrosion (MIC) of 304 stainless steel induced by <em>Rhodopseudomonas palustris</em> TIE-1. Riboflavin accelerated the MIC process, deepening and expanding corrosion pits. Electrochemical results showed a significant increase in corrosion rate, especially with the addition of 40 ppm riboflavin. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the passive film underwent a compositional transformation from Fe<sub>2</sub>O<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub>/Cr<sub>2</sub>O<sub>3</sub> to FeOOH/Cr(OH)<sub>3</sub>, accompanied by oxidative conversion of Cr<sub>2</sub>O<sub>3</sub> to CrO<sub>3</sub> mediated by riboflavin-facilitated extracellular electron uptake (EEU). High performance liquid chromatography (HPLC) results confirmed riboflavin degradation into lumichrome, which accelerated extracellular electron transfer (EET). Scanning electrochemical microscopy (SECM) analysis demonstrated that lumichrome's redox cycling enhanced EEU efficacy, resulting in the degradation of passive film.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108968"},"PeriodicalIF":4.8,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Initial quality assessment and qualitative interpretation of protein film electrochemistry catalytic data

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-08 DOI: 10.1016/j.bioelechem.2025.108967

Miriam Malagnini, Anna Aldinio-Colbachini, Laura Opdam, Andrea Di Giuliantonio, Andrea Fasano, Vincent Fourmond, Christophe Léger

引用次数: 0

Magnetic MOF-based sensing platform integrated with graphene field-effect transistors for ultrasensitive detection of infectious disease

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-03-06 DOI: 10.1016/j.bioelechem.2025.108951

Yushuang Liu , Mingxuan Wang , Guiqi Zhou , Ying Zhang , Wenfeng Hai

引用次数: 0