Bioelectrochemistry最新文献_第10页

Numerical modeling of giant pore formation in vesicles under msPEF-induced electroporation: Role of charging time and waveform mspef诱导电穿孔下囊泡大孔形成的数值模拟：充电时间和波形的作用

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-07 DOI: 10.1016/j.bioelechem.2025.108926

Nalinikanta Behera, Rochish M. Thaokar

{"title":"Numerical modeling of giant pore formation in vesicles under msPEF-induced electroporation: Role of charging time and waveform","authors":"Nalinikanta Behera, Rochish M. Thaokar","doi":"10.1016/j.bioelechem.2025.108926","DOIUrl":"10.1016/j.bioelechem.2025.108926","url":null,"abstract":"<div><div>Giant unilamellar vesicle is the closest prototypical model for investigating membrane electrodeformation and electroporation in biological cells. This work employs numerical simulations to investigate the effect of membrane charging time on vesicle electroporation under milli-second pulsed-electric-field (msPEF) of different waveforms. Our numerical approach, which implements the effect of electric stretching on membrane tension and precise calculation of pore energy, successfully predicts the formation of giant pores of <span><math><mrow><mi>O</mi><mrow><mo>(</mo><mn>1</mn><mo>)</mo></mrow></mrow></math></span> <span><math><mrow><mi>μ</mi><mi>m</mi></mrow></math></span> size as observed in previous experiments. The poration zone is found to extend up to certain angles as measured from the poles, termed critical angles. An increase in charging time delays pore formation, decreases the pore density, and trims down the poration zone. Counterintuitively, this effect promotes significant pore growth. Moreover, there exists a cut-off charging time above which pore formation is completely inhibited. This phenomenon is particularly pronounced with square bipolar pulses. Comparisons with the previous experimental results reveal that electrodeformation-poration-induced membrane surface area variation and that induced only by electroporation evolves in a similar fashion. Therefore, although the agreements are qualitative, the present electroporation model can be used as the simplest tool to predict the evolution of vesicles under electric pulses in laboratory experiments.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108926"},"PeriodicalIF":4.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid bacterial detection through microfluidic integration with a glucometer 微流控集成血糖仪快速细菌检测

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-06 DOI: 10.1016/j.bioelechem.2025.108936

Merve Eryilmaz , Sibel Ilbasmis-Tamer , Sallahuddin Panhwar , Emine Kübra Tayyarcan , İsmail Hakkı Boyaci , Zekiye Suludere , Demet Çetin , Adem Zengin , Ender Yıldırım , Uğur Tamer

{"title":"Rapid bacterial detection through microfluidic integration with a glucometer","authors":"Merve Eryilmaz , Sibel Ilbasmis-Tamer , Sallahuddin Panhwar , Emine Kübra Tayyarcan , İsmail Hakkı Boyaci , Zekiye Suludere , Demet Çetin , Adem Zengin , Ender Yıldırım , Uğur Tamer","doi":"10.1016/j.bioelechem.2025.108936","DOIUrl":"10.1016/j.bioelechem.2025.108936","url":null,"abstract":"<div><div>We present a novel approach for sensitive and portable detection of pathogenic bacteria, which is crucial for household and clinical practice. Our method employs immunoliposomes, antibodies, and microchip to detect specific pathogens quantitatively. Gold and metal metal–organic nanoparticles and liposomes were characterized using high-resolution techniques like TEM and SEM. Utilizing a commercial, personal glucose meter (PGM), we initially detected released glucose from antibody-modified liposomes and microchips with MOF-NPs. Detection on the microchip was achieved within 30 min, while the PGM analysis took only one minute for targeted bacteria, yielding glucose signals of 66 mg/dL and 69 mg/dL, respectively. Serial dilutions with group A-<em>Streptococcus pyogenes</em> (GAS) (1.4 × 10<sup>^4</sup>–1.4 × 10<sup>^8</sup> CFU/mL) demonstrated quantitative measurement applicability. This innovative approach and a portable PGM hold promise for various industries, including physician labs, hospitals, and households.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108936"},"PeriodicalIF":4.8,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of pulsed electric field (PEF) as a strategy to enhance aminoglycosides efficacy against Gram-negative bacteria 应用脉冲电场（PEF）提高氨基糖苷类药物对革兰氏阴性菌的药效

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-05 DOI: 10.1016/j.bioelechem.2025.108935

Mindaugas Visockis, Paulius Ruzgys, Simona Gelažunaitė, Salvijus Vykertas, Saulius Šatkauskas

{"title":"Application of pulsed electric field (PEF) as a strategy to enhance aminoglycosides efficacy against Gram-negative bacteria","authors":"Mindaugas Visockis, Paulius Ruzgys, Simona Gelažunaitė, Salvijus Vykertas, Saulius Šatkauskas","doi":"10.1016/j.bioelechem.2025.108935","DOIUrl":"10.1016/j.bioelechem.2025.108935","url":null,"abstract":"<div><div>In this study, two aminoglycosides (AGs), Kanamycin and Gentamicin, with similar modes of action and molecular weights, were combined with PEF treatment to enhance the inactivation of <em>E. coli</em> cells. Various PEF strengths were applied to assess the combined effect. To compare the inactivation efficacy of different AGs, bacterial growth measurements in suspension were performed at 3 and 10 h intervals over a 10-h period after PEF treatment. Interestingly, it was found that the additive effect of PEF treatment on <em>E. coli</em> growth inhibition was significantly greater with Kanamycin (IC<sub>50</sub>) than with Gentamicin (IC<sub>50</sub>). Further analysis revealed that the combined treatment with Kanamycin (IC<sub>50</sub>) was most effective within a timeframe of around 3 h. Our findings suggest that PEF treatment can significantly enhance the efficacy of AGs against Gram-negative bacteria; however, the extent of the additive effect varies depending on the specific antibiotic and the intensity of the applied PEF treatment.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108935"},"PeriodicalIF":4.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A microfluidic co-culture platform for lung cancer cells electrotaxis study under the existence of stromal cells 间质细胞存在下肺癌细胞电趋向性研究的微流控共培养平台

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-03 DOI: 10.1016/j.bioelechem.2025.108917

Lina Zheng, Keying Li, Xianmei Tang, Cuiping Li, Hailiang Nie, Lirong Han, Yaping Li

{"title":"A microfluidic co-culture platform for lung cancer cells electrotaxis study under the existence of stromal cells","authors":"Lina Zheng, Keying Li, Xianmei Tang, Cuiping Li, Hailiang Nie, Lirong Han, Yaping Li","doi":"10.1016/j.bioelechem.2025.108917","DOIUrl":"10.1016/j.bioelechem.2025.108917","url":null,"abstract":"<div><div>Tumor metastasis is an important reason for the poor prognosis and high mortality in cancer patients. As major component of stromal cells in tumor microenvironment, cancer-associated fibroblasts (CAFs) secreted various factors to promote tumor metastasis. Studies have indicated that endogenous direct current electric field (dcEF) around tumor tissue induced directional migration of cancer cells. However, the regulatory effect of CAFs on cancer migration under dcEF stimulation is still unknown. In this study, a two-layers polydimethylsiloxane (PDMS)-based microfluidic chip was fabricated. The introduction of concave structures achieved the non-contacted co-culture of different cell types, and parallel channels in the chip provided stable and homogeneous dcEF. Cells electrotactic response was evaluated under co-culture circumstance. The results showed that CAFs exhibited directional migration towards anode under dcEF stimulation, while A549 cells had a trend of directional migration towards cathode. The co-existence of CAFs and dcEF significantly enhanced the motility and cathodal migration of A549 cells, suggesting synergistic influences of chemotaxis from CAFs and electrotaxis from dcEF stimulation. Moreover, we demonstrated that lung normal fibroblasts acquired CAFs properties after stimulated by dcEF, characterizing by increasing gene expression of α-SMA and IL-6. Overall, Our device and study provide new insight for tumor electrotaxis in complex microenvironment.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108917"},"PeriodicalIF":4.8,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143155968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Molecular monolayers on silicon as substrates for biosensors” [Bioelectrochem. 80(1) (2010) 17–25] 硅上的分子单层作为生物传感器的基底"[Bioelectrochem.

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.bioelechem.2024.108849

L. Touahir , P. Allongue , D. Aureau , R. Boukherroub , J.-N. Chazalviel , E. Galopin , A.C. Gouget-Laemmel , C. Henry de Villeneuve , A. Moraillon , J. Niedziółka-Jönsson , F. Ozanam , J. Salvador Andresa , S. Sam , I. Solomon , S. Szunerits

引用次数: 0

Emerging screening platform characterises aminoquinoline structure–activity relationships with phospholipid layers 新兴的筛选平台表征氨基喹啉与磷脂层的构效关系

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.bioelechem.2025.108927

Bethany Crow , Roland Grafstrom , Vesa Hongisto , Mitali Kamat , Nikil Kapur , Ross Kelly , Josh Owen , Ashi Rashid , William Stokes , Nicola William , Jeanine Williams , Andrew Nelson

{"title":"Emerging screening platform characterises aminoquinoline structure–activity relationships with phospholipid layers","authors":"Bethany Crow , Roland Grafstrom , Vesa Hongisto , Mitali Kamat , Nikil Kapur , Ross Kelly , Josh Owen , Ashi Rashid , William Stokes , Nicola William , Jeanine Williams , Andrew Nelson","doi":"10.1016/j.bioelechem.2025.108927","DOIUrl":"10.1016/j.bioelechem.2025.108927","url":null,"abstract":"<div><div>Aminoquinolines (AQ) and substituted aminoquinolines (<em>s</em>-AQ) interact with electrochemically monitored supported dioleoyl phosphatidylcholine (DOPC) monolayers and immobilised artificial membranes (IAM) on HPLC column. The electrochemical sensor records adsorption/partition of the compound on and into the layer as well as specific interactions due to the location of the compound in the layer. HPLC-IAM technology measures the partition coefficient between the solution and phospholipid including partition due to interaction of the positive molecular charge with the phospholipid polar heads. The monolayer interaction results were combined and normalised for the neutral compounds’ lipophilicity as a log biomembrane affinity index (‘log BAI’) to exemplify charge and structural features in the interaction. A ChimeraX molecular modelling procedure was used to aid in the results interpretation. A compound ToxScore value was derived from 5 <em>in vitro</em> assays. The ‘log BAI’ exhibited a linear relationship with the AQ pK<sub>a</sub> values showing that the interaction was related to the molecular positive charge and to the electron donating properties of the –NH<sub>2</sub> group. The correlation outliers showed a tendency/no tendency to H-bonding with the polar groups and a superficial/deeper location respectively in the phospholipid layer. The <em>s</em>-AQ ‘log BAI’ value displayed a power correlation with the compounds’ ToxScore values.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108927"},"PeriodicalIF":4.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143354720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Analysis of electromagnetic response of cells and lipid membranes using a model-free method” [Bioelectrochemistry 152 (2023) 108444] 对 "利用无模型方法分析细胞和脂膜的电磁响应"[生物电化学 152 (2023) 108444] 的更正。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-02-01 DOI: 10.1016/j.bioelechem.2024.108841

Yingxian Lu , Xiaping Tang , Yanyu Zhao , Tianyu Jiang , Jiayao Zhou , Xiaofei Wang , Bing Huang , Lingyu Liu , Hu Deng , Yujing Huang , Yigong Shi

引用次数: 0

Promotion of quiescence and maintenance of function of mesenchymal stem cells on substrates with surface potential 在具有表面电位的基质上促进间充质干细胞的静止和维持其功能

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-31 DOI: 10.1016/j.bioelechem.2025.108920

Xiaoshuai Peng , Guojian Li , Jiu Zhao , Huatao Liu , Changhua Wu , Zepeng Su , Zhidong Liu , Shuai Fan , Yuanquan Chen , Yanfeng Wu , Wenjie Liu , Huiyong Shen , Guan Zheng

{"title":"Promotion of quiescence and maintenance of function of mesenchymal stem cells on substrates with surface potential","authors":"Xiaoshuai Peng , Guojian Li , Jiu Zhao , Huatao Liu , Changhua Wu , Zepeng Su , Zhidong Liu , Shuai Fan , Yuanquan Chen , Yanfeng Wu , Wenjie Liu , Huiyong Shen , Guan Zheng","doi":"10.1016/j.bioelechem.2025.108920","DOIUrl":"10.1016/j.bioelechem.2025.108920","url":null,"abstract":"<div><div>The widespread use of human mesenchymal stem cells(hMSCs) is impeded by functional loss during prolonged expansion. Although multiple approaches have been attempted to preserve hMSCs stemness, a suitable culture system remains to be modified. The interaction between electrical signals and stem cells is expected to better maintain the function of stem cells. However, it remains unclear whether the surface potential of substrates has the potential to preserve stem cell function during in vitro expansion. In our study, hMSCs cultured on materials with different surface potentials could be induced into a reversible quiescent state, and we demonstrated that quiescent hMSCs could be reactivated and transitioned back into the proliferation cell cycle. hMSCs cultured under appropriate potential displayed superior differentiation and proliferation abilities within the same generation compared to conventional conditions. These findings underscore the importance of surface potential as a critical physical factor regulating hMSCs stemness. Manipulating the surface potential of hMSCs culture substrates holds promise for optimising preservation and culture conditions, thereby enhancing their application in tissue repair and regeneration engineering.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108920"},"PeriodicalIF":4.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143154584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CeO2@Co3O4-decorated carbon felt anode with mesoporous nanoparticles for enhanced POM wastewater treatment and electricity generation in microbial fuel cells CeO2@Co3O4-decorated碳毡阳极与介孔纳米颗粒增强POM废水处理和发电的微生物燃料电池。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-31 DOI: 10.1016/j.bioelechem.2025.108924

Zhiguo Gao, Ping Xue, Linmeng Ma, Keren Shi, Haibo Wang

{"title":"CeO2@Co3O4-decorated carbon felt anode with mesoporous nanoparticles for enhanced POM wastewater treatment and electricity generation in microbial fuel cells","authors":"Zhiguo Gao, Ping Xue, Linmeng Ma, Keren Shi, Haibo Wang","doi":"10.1016/j.bioelechem.2025.108924","DOIUrl":"10.1016/j.bioelechem.2025.108924","url":null,"abstract":"<div><div>Microbial fuel cells (MFCs) have been widely used in the treatment of organic wastewater because of its advantages of high efficiency, environmental friendliness, and no secondary pollution. In this study, we developed a composite anode, CeO<sub>2</sub>@Co<sub>3</sub>O<sub>4</sub>-PEDOT/CF (PEDOT: poly(3,4-ethylenedioxythiophene), CF: carbon felt), by incorporating bimesoporous CeO<sub>2</sub>@Co<sub>3</sub>O<sub>4</sub> nanoparticles and PEDOT layer. The MFCs equipped with this novel anode had demonstrated exceptional catalytic performance in the treatment of Polyoxymethylene (POM) wastewater. Specifically, it achieved a 74.9 % increase in output voltage (586 ± 23 mV), a 2.5-fold increase in power density (3564 ± 27 mW/m<sup>2</sup>), and enhancements of 41.8 %, 40.1 %, and 47.0 % in the degradation rates of formaldehyde (HCHO, 97.2 %), 1,3,5-trioxane (TOX, 93.6 %), and chemical oxygen demand (COD) removal efficiency (84.4 %), respectively, when compared to a bare CF anode. Furthermore, the MFC with the composite anode demonstrated high degradation efficiency and COD removal efficiency across various industrial water environments. Additionally, the wheat seed germination experiments indicated a significant reduction in the toxicity of the degraded solution. This enhanced performance could be attributed to the synergistic effects of the bimesoporous CeO<sub>2</sub>@Co<sub>3</sub>O<sub>4</sub> nanoparticles and the PEDOT layer. This study presents a promising strategy for the degradation of organic pollutants and the generation of bioelectricity in practical applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108924"},"PeriodicalIF":4.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical and computational studies on the interaction between calf-thymus DNA and skin whitening agent arbutin 小牛胸腺DNA与皮肤增白剂熊果苷相互作用的电化学和计算研究。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-30 DOI: 10.1016/j.bioelechem.2025.108923

Maryam M.M. Mahdi, Alper Fitoz, Ceren Yıldız, Dilek Eskiköy Bayraktepe, Zehra Yazan

{"title":"Electrochemical and computational studies on the interaction between calf-thymus DNA and skin whitening agent arbutin","authors":"Maryam M.M. Mahdi, Alper Fitoz, Ceren Yıldız, Dilek Eskiköy Bayraktepe, Zehra Yazan","doi":"10.1016/j.bioelechem.2025.108923","DOIUrl":"10.1016/j.bioelechem.2025.108923","url":null,"abstract":"<div><div>The interaction between double-stranded calf thymus DNA (<em>ctDNA</em>) and the skin whitening agent arbutin (<em>AR</em>) examined by applying electrochemical and computational methods for the first time in literature. A single-use pencil graphite electrode via cyclic (<em>CV</em>) and differential pulse voltammetry (<em>DPV</em>) techniques were applied to determine the kinetic and thermodynamic parameters in the absence and presence of <em>ctDNA</em>. To examine the interaction process, oxidation peak currents and potentials of <em>AR</em> were observed prior to the addition of various <em>ctDNA</em> concentrations. The binding constants (<em>K<sub>AR-DNA</sub></em>) and Gibbs free energy (Δ<em>G°</em>) values for the <em>AR-DNA</em> complex were determined as <em>1.82 × 10</em><sup>4</sup> <em>L/mol</em> and <em>−24.30 kJ/mol</em> at 298 K, respectively. Temperature evaluation of the interaction was examined using thermodynamic parameters (Δ<em>H°: −30.30 kJ/mol</em> and Δ<em>S°: −0.00197 kJ/mol</em>) applying the Van’t Hoff equation. The local interaction sites in the molecule structure were determined by applying Fukui functions and second-order perturbation theory in view of potential hydrogen binding centers. The optimized structure of <em>AR</em> was applied with a <em>DNA</em> structure revealing the binding position for <em>AR-DNA</em> complex. Experimental and computational examinations suggested that <em>AR-DNA</em> binds to <em>ctDNA</em> through a minor groove mode via conventional hydrogen bonds, hydrophobic interactions and van der Waals forces.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108923"},"PeriodicalIF":4.8,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0