Bioelectrochemistry最新文献

{"title":"Highly stretchable, adhesive and conductive hydrogel for flexible and stable bioelectrocatalytic sensing layer of enzyme-based amperometric glucose biosensor","authors":"Linghui Tang ,&nbsp;Yufeng Huang ,&nbsp;Yue Wang ,&nbsp;Jifan Zhao ,&nbsp;Huiyong Lian ,&nbsp;Yan Dong ,&nbsp;Zhiqiang Zhang ,&nbsp;Yasushi Hasebe","doi":"10.1016/j.bioelechem.2024.108882","DOIUrl":"10.1016/j.bioelechem.2024.108882","url":null,"abstract":"<div><div>Highly stretchable, adhesive and conductive triblock hydrogel was synthesized and utilized as a flexible and stable bioelectrocatalytic sensing layer of enzyme-based amperometric glucose biosensor. The hydrogel was prepared through one-pot polymerization of 2-acrylamido-2-methyl-1-propanesulfonic acid, methacrylamide, and hydroxyethyl methacrylate. The physical and chemical properties of the hydrogel were characterized with X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and electrochemical techniques. Glucose oxidase (GOx) and chitosan (CTS) embedded hydrogel was drop-coated on glassy carbon electrode (GCE) and screen printed graphite electrode (SPGE). The resulting GOx/CTS/hydrogel-GCE and GOx/CTS/hydrogel-SPGE exhibited excellent mediated bioelectrocatalytic oxidation current for glucose. The calibration curve of glucose by the GOx/CTS/hydrogel-GCE showed the linear range from 0.25 to 15 mM with the sensitivity of 27.0 µA mM⁻¹ cm⁻². This GOx/CTS/hydrogel-based sensing layer coated on the SPGE was stable against bending, and the response to glucose was almost same irrespective of the bending angles (0, 30, 60, and 90 degree). In addition, the response to glucose was not interfered by various organic and inorganic interfering species, allowed to detect glucose in goat serum. Furthermore, the GOx/CTS/hydrogel-GCE kept its original activity of 99.64 % during 30 days’ storage under dry state in refrigerator.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108882"},"PeriodicalIF":4.8,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced electrochemical aptasensor integrating MoS2/CuS-Au and SI-RAFT for dual signal amplification in cTnI detection","authors":"Yuning Zhao ,&nbsp;Xiaojing Cui ,&nbsp;Yilong Wang ,&nbsp;Zhendong Wang ,&nbsp;Xiaofei Li ,&nbsp;Ying Wang ,&nbsp;Huaixia Yang ,&nbsp;Yanju Liu ,&nbsp;Mingsan Miao","doi":"10.1016/j.bioelechem.2024.108862","DOIUrl":"10.1016/j.bioelechem.2024.108862","url":null,"abstract":"<div><div>Cardiac troponin I (cTnI) is known to be among the prominent diagnostic bio-marker for acute myocardial infarction (AMI). In this paper, we proposed an electrochemical aptasensor with nanomaterial MoS₂/CuS-Au as the substrate material and perillaldehyde (PA) as the surface-initiated reversible addition fragmentation chain transfer (SI-RAFT) polymerization monomer by mediating the in-situ deposition of silver metal on the electrode surface for ultrasensitive detection of cTnI. The substrate material MoS₂/CuS-Au not only accelerated the rate transfer between electron, but also provided more active sites for aptamers introduction. The perillaldehyde (PA) monomers were modified onto the electrode by surface-initiated reversible addition fragmentation chain transfer polymerization (SI-RAFT), and the signal was amplified in one step by reducing silver ions to metallic silver deposited in situ on the upper of the electrode surface. Under optimum reaction conditions, this aptasensor achieved a detection limit down to 10.83 fg·mL⁻¹. The proposed aptasensor without additional natural enzymes or biological tags, had a promising potential for use in the diagnosis of early acute myocardial infarction (AMI).</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108862"},"PeriodicalIF":4.8,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro sensing of traumatic brain tissue by electrochemical impedance for diagnosis and therapeutic","authors":"Sebastinbaskar Aniu Lincy ,&nbsp;Yesurajan Allwin Richard ,&nbsp;Jeyaraj Jeyavani ,&nbsp;Baskaralingam Vaseeharan ,&nbsp;Venkataraman Dharuman","doi":"10.1016/j.bioelechem.2024.108871","DOIUrl":"10.1016/j.bioelechem.2024.108871","url":null,"abstract":"<div><div>Traumatic brain injury (TBI) induces neurogenerative disorders affecting severely daily human activities and early diagnosis is a critical requirement for prevention and cure. Here, we induced TBI formation in the Zebra fish, a model organism, by both mechanical (ultrasonic cleaner generated convulsive shock, UGCS) and chemical (pentylenetetrazol, PTZ) methods. The TBI induced cellular and neuronal changes are monitored by measuring the activities of the indicator biomarkers viz., superoxide anion (O₂^<img>−) and glutamate by electrochemical techniques. For this, the α-lipoic acid (α-LA, LA) functionalized gold-silver (LA-Au/Ag) is used as an electrochemical sensor to diagnose the presence of these markers in physiological phosphate buffer saline (PBS, <em>pH</em> 7.4), 0.1 M KCl solutions and in TBI tissues. While the oxidation of glutamate is observed in the potential window 0.2–0.5 V, the metal mediated oxidation of O₂^<img>− is observed at the potential window 0.6–1.0 V. The sensor showed good linear ranges for O₂^<img>− (from 4 to 48 μM with the LOD of 4 μM for the O₂^<img>− detection) and glutamate (from 20 to 130 μM with the LOD 19 μM). The TBI tissue modified electrode showed lower resistance than the normal brain tissue ((NBT), as control) due to the presence of higher amount of O₂^<img>− and occurrence of Fenton’s and Heber-Weise’s reactions in the presence of [Fe(CN)₆]^3−/4−. For theragnostic application, the LA-Au/Ag nanoparticles is delivered into the UGCS and PTZ treated Zebrafish and electrochemical signal changes are monitored by cyclic voltammetry and impedance spectroscopy. Electrochemical data further corroborated with the activities of superoxide dismutase (SOD), Catalase (CAT) and lipid peroxidase (MDA) in parallel. The developed method of electrochemical sensing of TBI may provide alternative for the early TBI diagnosis and therapeutics for the prevention of TBI.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108871"},"PeriodicalIF":4.8,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of label-free immunosensors based on AuNPs-fullerene nanocomposites for the determination of cancer antigen 125","authors":"Ulviye Kilimci ,&nbsp;Baha Öndeş ,&nbsp;Çağdaş Sunna ,&nbsp;Murat Uygun ,&nbsp;Deniz Aktaş Uygun","doi":"10.1016/j.bioelechem.2024.108863","DOIUrl":"10.1016/j.bioelechem.2024.108863","url":null,"abstract":"<div><div>In this study, gold nanoparticles (AuNPs) were synthesized and combined with fullerene, resulting in the formation of nanocomposite structures. The structures were then characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) techniques. The nanostructures were functionalized with MPA and employed for covalent binding of CA125 antibody, whereby the antibody-bound nanocomposite structure was utilized for modification of the surface of the SPE. The surface of the immunosensor was protected by Nafion, and the individual stages of the immunosensor design were characterized by CV and EIS. CA125 determination was conducted using the EIS technique, which revealed a linear concentration range of 1–100 U·mL⁻¹ and a LOD value of 0.016 U·mL⁻¹. The immunosensor demonstrated selective recognition of CEA, NSE, HSA, and IgG proteins, exhibiting good reproducibility. The prepared immunosensor demonstrated 80.9% activity even after a 30-day period. Moreover, this immunosensor can be successfully employed in conventional clinical human serum applications. A comparison with existing literature reveals that the superior features of this immunosensor are its low LOD and high stability. Additionally, the short analysis time in comparison to commercial kits is considered a significant advantage. The prepared immunosensor displays valuable characteristics for the determination of CA125, and it has the potential to be developed for use in health applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108863"},"PeriodicalIF":4.8,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of preferential growth of Shewanella oneidensis MR-1 on microbial corrosion of constituent phases of 2205 duplex stainless steel","authors":"Mohammed Arroussi ,&nbsp;Jiajia Wu ,&nbsp;Liyang Zhu ,&nbsp;Peng Wang ,&nbsp;Dun Zhang ,&nbsp;Wenkai Wang","doi":"10.1016/j.bioelechem.2024.108859","DOIUrl":"10.1016/j.bioelechem.2024.108859","url":null,"abstract":"<div><div>Understanding selective growth of electroactive bacteria on surface of constituent phases (ferrite/ austenite) of 2205 DSS is required for mitigating microbiologically influenced corrosion (MIC). In this study, the preferential attachment of bacteria and its impact on corrosion of single phase were investigated under anaerobic condition using <em>Shewanella oneidensis</em> MR-1. Single-ferrite phase was more susceptible to biofilm formation compared with single-austenite phase. Atomic force microscope (AFM) revealed that the surface of ferrite phase coupon was fully covered with <em>S. oneidensis</em> MR-1 biofilm whereas few <em>S. oneidensis</em> MR-1 cells were observed on the surface of austenite phase. After 14 d of incubation, the maximum biofilm thicknesses on 2205 DSS, ferrite and austenite phase were 15.5 ± 1.0 µm, 13.8 ± 3.2 µm, and 10.2 ± 0.8 µm, respectively. <em>S. oneidensis</em> MR-1 accelerated the pitting corrosion of materials. The maximum pits depth on single ferrite and austenite phase in sterile medium (3.2 µm vs 2.2 µm with mean values 2.5 µm vs 1.7 µm) were relatively small than those in biotic medium (6.0 µm vs 4.5 µm with mean values 4.5 µm vs 3.8 µm). Synergistic effects of Cr and Ni enhanced the stability of passive film on austenite phase.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108859"},"PeriodicalIF":4.8,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper nanocluster based cascade amplified DNA electrochemical detection combining with bio-barcode assay and surface-initiated enzyme polymerization","authors":"Zhao Huang ,&nbsp;Kaimin Wu ,&nbsp;Feiye Ju ,&nbsp;Ran He ,&nbsp;Ying Tang ,&nbsp;Yao Chen ,&nbsp;Xuliang He ,&nbsp;Jing Zhang ,&nbsp;Libo Nie","doi":"10.1016/j.bioelechem.2024.108857","DOIUrl":"10.1016/j.bioelechem.2024.108857","url":null,"abstract":"<div><div>Early cancer diagnosis is paramount for enhancing treatment efficacy, extending patient survival, and improving the quality of life. We developed a highly sensitive electrochemical biosensor for the detection of target DNA (tDNA) associated with gastric cancer. This advancement integrates dual signal amplification strategies: bio-barcode amplification (BCA) and surface-initiated enzyme polymerization (SIEP), with copper nanoclusters (CuNCs) serving as signal labels. Silica nanoparticles (SiO₂) were covalently linked with polythymine (poly T) and complementary DNA to create bio-barcode probes. These probes, through hybridization, were immobilized on the reduced graphene oxide and Au nanoparticle (rGO-AuNPs) modified interface and marking the first amplification of the electrical signal. Subsequently, the extended poly T prompted by SIEP bound additional CuNCs through the combination of T-Cu²⁺, leading to a second round of signal amplification. The biosensor demonstrated a minimum detection limit of 0.13 fmol/L over a linear response range from 1 fmol/L to 1 nmol/L. It also showcased excellent specificity, repeatability, and stability, making it a promising tool for the sensitive detection of gastric cancer biomarkers.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108857"},"PeriodicalIF":4.8,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142759075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Voltammetric analysis of glycoproteins containing sialylated and neutral glycans at pyrolytic graphite electrode","authors":"Mojmír Trefulka,&nbsp;Hana Černocká,&nbsp;Tatiana Staroňová,&nbsp;Veronika Ostatná","doi":"10.1016/j.bioelechem.2024.108851","DOIUrl":"10.1016/j.bioelechem.2024.108851","url":null,"abstract":"<div><div>Recently, it was described that neutral glycans can be distinguished from those containing sialic acid at the mercury electrode after modification with osmium(VI) <em>N</em>,<em>N</em>,<em>N</em>',<em>N</em>'-tetramethylethylenediamine (Os(VI)tem). Our work shows the possibility of studying glycans and glycoproteins at pyrolytic graphite electrodes depending on the<!--> <!-->presence of sialic acid.</div><div>Short glycans, glycans released from glycoproteins, and glycoproteins themselves yielded similar voltammetric responses after their modification by Os(VI)tem. Os(VI)tem modified glycans and glycoproteins produced a<!--> <!-->couple of cathodic and anodic peaks. Changing peak heights and potentials of glycans and glycoproteins pointed out the presence of sialic acid. These findings could be utilized to improve glycoprotein sensing by chemical modification.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108851"},"PeriodicalIF":4.8,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free determination of glypican-3 using PtPd@H-rGO nanocomposites decorated light-addressable potentiometric sensor","authors":"Guiyin Li ,&nbsp;Wenzhan Li ,&nbsp;Yu Zhou ,&nbsp;Xiaohong Tan ,&nbsp;Qing Huang ,&nbsp;Jintao Liang ,&nbsp;Zhide Zhou","doi":"10.1016/j.bioelechem.2024.108855","DOIUrl":"10.1016/j.bioelechem.2024.108855","url":null,"abstract":"<div><div>Glypican-3 (GPC3) is exclusively overexpressed in most Hepatocellular carcinoma (HCC) tissue but not in normal liver tissue, making it a promising biomarker for the precise detection of HCC. In this paper, a label-free light-addressable potentiometric sensor (LAPS) decorated by platinumpalladium-hemin-reduced graphene oxide nanocomposites (PtPd@H-rGO NCs) was constructed for determination of GPC3. The GPC3 aptamer (GPC3_Apt) and PtPd@H-rGO NCs were modified on the surface of silicon-based LAPS chip to build sensitive unit of LAPS system. A readout photocurrent elicited from a modulated light source, registers the localized surface potential change. When a bias voltage is provided to the LAPS system, the GPC3-GPC3_Apt complexes formed by the specific reaction between GPC3 and GPC3_Apt at the sensing interface can cause the sensitive membrane surface potential to change, resulting in the photocurrent-voltage (I-V) curves generate a corresponding offset response. Therefore GPC3 concentration can be determined by monitoring the potential shifts (△V). Under optimal conditions, the potential shift is linearly related to the concentration of GPC3 in the range of 0.001–3.00 μg/mL with the limit of detection (LOD) of 0.0001 μg/mL. The LAPS has a good analytical performance with good specificity, reproducibility and stability, and can be used for the detection of GPC3 in actual serum samples, which provides a broad application prospect for the combined application of LAPS and aptamers in biooassay.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108855"},"PeriodicalIF":4.8,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical immunosensors based on the solubility difference of electroactive probe and the dual signal amplification of nanocarrier plus redox cycling","authors":"Yong Chang ,&nbsp;Yajun Wang ,&nbsp;Xueqian Fan ,&nbsp;Jia Zhou ,&nbsp;Yunhe Lv ,&nbsp;Ning Xia","doi":"10.1016/j.bioelechem.2024.108858","DOIUrl":"10.1016/j.bioelechem.2024.108858","url":null,"abstract":"<div><div>This work reported a redox cycling system for the design of electrochemical immunosensors by using pyrroloquinoline quinone (PQQ) to promote the oxidation of tris(2-carboxyethyl)phosphine (TCEP). The consumption of TCEP was monitored with ferrocenium (Fc⁺) as the electroactive probe, which was based on the difference in the solubility of Fc⁺ with its reduced format (ferrocene, Fc). Metal–organic framework (MOF) was used as the nanocarrier to load biotinylated recognition antibody and PQQ with recombinant streptavidin as the linker. In the absence of target, TCEP could reduce Fc⁺ into insoluble Fc aggregates, thus leading to the decrease in the electrochemical signal. Capture of target allowed for the attachment of antibody-modified MOF-PQQ on the sensing electrode, thus promoting the oxidation of TCEP by O₂ through the redox cycling. In this case, the reduction of Fc⁺ into insoluble Fc aggregates was limited, and Fc⁺ remained in the solution exhibited a high electrochemical signal. The peak current was linearly proportional to the target concentration in the range of 0.001–1 ng/mL with prostate specific antigen as an example. The work should be useful for the design of novel biosensors through the signal amplification of redox cycling.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108858"},"PeriodicalIF":4.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A DNA sensor based on CbAgo effector protein and on a dual electrochemical signal amplification strategy for B19 parvovirus detection","authors":"Siying Mao ,&nbsp;Zhiruo Yang ,&nbsp;Zhiyi Liu ,&nbsp;Yue Wang ,&nbsp;Yonghua Zeng ,&nbsp;Nicole Jaffrezic-Renault ,&nbsp;Zhipeng Zhang ,&nbsp;Yanming Dong ,&nbsp;Zhenzhong Guo","doi":"10.1016/j.bioelechem.2024.108860","DOIUrl":"10.1016/j.bioelechem.2024.108860","url":null,"abstract":"<div><div>Human parvovirus B19 is a prevalent childhood infectious virus that poses a great challenge to public health, so the detection of B19V is of great importance. In this study, a DNA sensor based on <em>Cb</em>Ago, a Cas effector, and a dual electrochemical signal amplification strategy was developed by using a novel nanocomposite MnO₂/CMK-3/g-C₃N₄/AgNPs for initial signal amplification, with CMK being an ordered mesoporous carbon nanomaterial. Single-walled carbon nanotubes (SWCNTs) were used as electrocatalytic probes for secondary signal amplification to detect B19 DNA. The detection process begins with polymerase chain reaction (PCR) amplification using the B19V infectious clone plasmid (pB19-M20) as a template and NS1-F/R as primers, followed by specific cleavage of B19 DNA based on the programmable cutting sites of <em>Cb</em>Ago effector protein. This study enriches the application of Argonaute proteins in sensing and introduces a novel method to detect B19V. Under optimized conditions, the biosensor can detect B19 DNA in the range of 10⁻¹⁵–10⁻¹⁰ M, with a detection limit (LOD) of 0.2 fM. The results indicate that the developed DNA sensor holds promise for reliable and sensitive detection of B19 DNA in human serum.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108860"},"PeriodicalIF":4.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}