Bioelectrochemistry最新文献_第10页

A sensitive tobramycin electrochemical aptasensor based on multiple signal amplification cascades 基于多重信号放大级联的灵敏妥布霉素电化学适配传感器

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-15 DOI: 10.1016/j.bioelechem.2024.108797

Yi Zhao , Qirong Chen , Yujie Liu , Bingying Jiang , Ruo Yuan , Yun Xiang

{"title":"A sensitive tobramycin electrochemical aptasensor based on multiple signal amplification cascades","authors":"Yi Zhao , Qirong Chen , Yujie Liu , Bingying Jiang , Ruo Yuan , Yun Xiang","doi":"10.1016/j.bioelechem.2024.108797","DOIUrl":"10.1016/j.bioelechem.2024.108797","url":null,"abstract":"<div><p>The residue of tobramycin, a broad spectrum antibiotic commonly used in animal husbandry, has evitable impact on human health, which may cause kidney damage, respiratory paralysis, neuromuscular blockade and cross-allergy in humans. Sensitive monitoring of tobramycin in animal-derived food products is therefore of great importance. Herein, a new aptamer electrochemical biosensor for sensing tobramycin with high sensitivity is demonstrated via exonuclease III (Exo III) and metal ion-dependent DNAzyme recycling and hybridization chain reaction (HCR) signal amplification cascades. Tobramycin analyte binds aptamer-containing hairpin probe to switch its conformation to expose the toehold sequence, which triggers Exo III-based catalytic digestion of the secondary hairpin to release many DNAzyme strands. The substrate hairpins immobilized on the Au electrode (AuE) are then cyclically cleaved by the DNAzymes to form ssDNAs, which further initiate HCR formation of lots of long methylene blue (MB)-tagged dsDNA polymers on the AuE. Subsequently electro-oxidation of these MB labels thus exhibit highly enhanced currents for sensing tobramycin within the 5–1000 nM concentration range with an impressive detection limit of 3.51 nM. Furthermore, this strategy has high selectivity for detecting tobramycin in milk and shows promising potential for detect other antibiotics for food safety monitoring.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"160 ","pages":"Article 108797"},"PeriodicalIF":4.8,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Respiration suppress and apoptosis-like cell death of Escherichia coli in direct current therapy mediated by polypyrrole conductive hydrogel 在聚吡咯导电水凝胶介导的直流治疗中抑制大肠杆菌的呼吸并使其细胞凋亡

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-15 DOI: 10.1016/j.bioelechem.2024.108796

Qinglong Ding , Bing Li , Xiao Fan , Wei Wang

{"title":"Respiration suppress and apoptosis-like cell death of Escherichia coli in direct current therapy mediated by polypyrrole conductive hydrogel","authors":"Qinglong Ding , Bing Li , Xiao Fan , Wei Wang","doi":"10.1016/j.bioelechem.2024.108796","DOIUrl":"10.1016/j.bioelechem.2024.108796","url":null,"abstract":"<div><p>Targeting oxidative phosphorylation of bacteria is a novel antibiotic strategy leading to rapid cell death as a result of respiration suppress. Herein, a conductive polymer termed polypyrrole (PPy) is used to short-circuit the electron transfer chain (ETC) of bacteria cells owing to its higher electron affinity to electrons than all of the electron carriers on ETC. A hydrogel is fabricated using PPy which is anticipated to seize electrons from ETC and inhibit respiration of bacteria cells. The results show that the prepared PPy hydrogel can mediate an effective direct current (DC) antibacterial therapy which greatly enhances intracellular reactive oxygen species (ROS) level of Escherichia coli (<em>E. coli</em>), suppresses respiration, induces apoptosis-like cell death of <em>E. coli</em> accompanied by chromosomal condensation and loss of structural integrity, and rapidly cleared <em>E. coli</em> infection <em>in vivo</em>. Taken into the photothermal property of PPy, a combined direct current-photothermal therapy is developed which can enhance bacteria-killing effects with the assistance of an 808 nm laser. Our findings provide a new antibiotic strategy with metabolic pathway as a target.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108796"},"PeriodicalIF":4.8,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001580/pdfft?md5=820b55846d3e78c8892b35c4a6c433e3&pid=1-s2.0-S1567539424001580-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluid biosensor for detection of hpv in patient scraping samples: Determining E6 and E7 oncogenes 用于检测患者刮片样本中 hpv 的微流控生物传感器：确定 E6 和 E7 致癌基因

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-13 DOI: 10.1016/j.bioelechem.2024.108795

Léony S. Oliveira , Sanket Goel , Khairunnisa Amreen , Norma Lucena-Silva , Maria D.L. Oliveira , Cesar A.S. Andrade

{"title":"Microfluid biosensor for detection of hpv in patient scraping samples: Determining E6 and E7 oncogenes","authors":"Léony S. Oliveira , Sanket Goel , Khairunnisa Amreen , Norma Lucena-Silva , Maria D.L. Oliveira , Cesar A.S. Andrade","doi":"10.1016/j.bioelechem.2024.108795","DOIUrl":"10.1016/j.bioelechem.2024.108795","url":null,"abstract":"<div><p>E6 and E7 oncogenes are pivotal in the carcinogenic transformation in HPV infections and efficient diagnostic methods can ensure the detection and differentiation of HPV genotype. This study describes the development and validation of an electrochemical, label-free genosensor coupled with a microfluidic system for detecting the E6 and E7 oncogenes in cervical scraping samples. The nanostructuring employed was based on a cysteine and graphene quantum dots layer that provides functional groups, surface area, and interesting electrochemical properties. Biorecognition tests with cervical scraping samples showed differentiation in the voltammetric response. Low-risk HPV exhibited a lower biorecognition response, reflected in ΔI% values of 82.33 % ± 0.29 for HPV06 and 80.65 % ± 0.68 for HPV11 at a dilution of 1:100. Meanwhile, high-risk, HPV16 and HPV18, demonstrated ΔI% values of 96.65 % ± 1.27 and 93 % ± 0.026, respectively, at the same dilution. Therefore, the biorecognition intensity followed the order: HPV16 >HPV18 >HPV06 >HPV11. The limit of detection and the limit of quantification of E6E7 microfluidic LOC−Genosensor was 26 fM, and 79.6 fM. Consequently, the E6E7 biosensor is a valuable alternative for clinical HPV diagnosis, capable of detecting the potential for oncogenic progression even in the early stages of infection.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"160 ","pages":"Article 108795"},"PeriodicalIF":4.8,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141987047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-performance electrolyte-gated amorphous InGaZnO field-effect transistor for label-free DNA sensing 用于无标记 DNA 感测的高性能电解质门控非晶 InGaZnO 场效应晶体管

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-10 DOI: 10.1016/j.bioelechem.2024.108794

Hong Liu, Junxin Chen, Jin Hu, Jiajun Song, Peng Lin

引用次数: 0

Electrochemical aptasensor based on a dual signal amplification strategy of 1-AP-CNHs and ROP for highly sensitive detection of ERα 基于 1-AP-CNHs 和 ROP 双信号放大策略的电化学适配传感器，用于高灵敏度检测 ERα。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-07 DOI: 10.1016/j.bioelechem.2024.108793

Ziyan Kong , Yilong Wang , Zhendong Wang , Xiaofei Li , Huaixia Yang , Mingsan Miao , Liang Guo

{"title":"Electrochemical aptasensor based on a dual signal amplification strategy of 1-AP-CNHs and ROP for highly sensitive detection of ERα","authors":"Ziyan Kong , Yilong Wang , Zhendong Wang , Xiaofei Li , Huaixia Yang , Mingsan Miao , Liang Guo","doi":"10.1016/j.bioelechem.2024.108793","DOIUrl":"10.1016/j.bioelechem.2024.108793","url":null,"abstract":"<div><p>Estrogen receptor alpha (ERα) serves as a crucial biomarker for early breast cancer diagnosis. In this study, we proposed an electrochemical aptasensor with nanomaterial carbon nanohorns/gold nanoparticle composites (1-AP-CNHs/AuNPs) as the substrate, and the primary amine groups on the antibody initiated the ring-opening polymerization (ROP) of monomer amino acid-ferrocene (NCA-Fc) on the electrode surface for ultrasensitive detection of ERα. The composite of 1-AP-CNHs/AuNPs not only possessed more active sites, but also increased the specific surface area of the electrode and allowed a large amount of ferrocene polymer long chains to be grafted onto the electrode surface to achieve signal amplification. Under optimal conditions, the detection limit of the method was 11.995 fg mL<sup>−1</sup> with a detection range of 100 fg mL<sup>−1</sup>–100 ng mL<sup>−1</sup>. In addition, the biotin-streptavidin system was used to further improve the sensitivity of the sensor. Importantly, this approach could be applied for the practical detection of ERα in real samples.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"160 ","pages":"Article 108793"},"PeriodicalIF":4.8,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141915672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simulation study on electroporation of cancer cells in multicellular system 多细胞系统中癌细胞电穿孔模拟研究。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-06 DOI: 10.1016/j.bioelechem.2024.108789

Yu Zhang , Zhijun Luo , Yapeng Zhang , Fei Guo

引用次数: 0

A type of self-assembled and label-free DNA-modified electrochemical biosensors based on magnetic α-Fe2O3/Fe3O4 heterogeneous nanorods for ultra-sensitive detection of CYP2C19*3 一种基于磁性 α-Fe2O3/Fe3O4 异质纳米棒的自组装无标记 DNA 修饰电化学生物传感器，用于超灵敏检测 CYP2C19*3。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-06 DOI: 10.1016/j.bioelechem.2024.108792

Sihan Zhao , Peng Deng , Mingyi Ma , Zhihao Xu , Aolin He , Ruijiang Liu

{"title":"A type of self-assembled and label-free DNA-modified electrochemical biosensors based on magnetic α-Fe2O3/Fe3O4 heterogeneous nanorods for ultra-sensitive detection of CYP2C19*3","authors":"Sihan Zhao , Peng Deng , Mingyi Ma , Zhihao Xu , Aolin He , Ruijiang Liu","doi":"10.1016/j.bioelechem.2024.108792","DOIUrl":"10.1016/j.bioelechem.2024.108792","url":null,"abstract":"<div><p>CYP2C19*3 enzyme plays a pivotal role in drug metabolism and is tightly regulated by the CYP2C19*3 gene. Therefore, quantification of CYP2C19*3 gene holds paramount importance for achieving personalized medication guidance in precision medicine. In this project, the magnetic electrochemical biosensors were constructed for the ultra-sensitive detection of CYP2C19*3 gene. Employing magnetic α-Fe<sub>2</sub>O<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub>@Au as the matrixes for signal amplification, CYP2C19*3 complementary chains (c-ssDNA) were bound to their surfaces through gold-sulfur bonds with subsequent specific sites blockade by bovine serum albumin (BSA) to form the α-Fe<sub>2</sub>O<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub>@Au/c-ssDNA/BSA biosensors. This design enabled efficient biosensors separation, target gene capture, and self-assembly on the electrode surface, enhancing the response signal. The biosensors exhibited excellent capture capabilities with a wide linear range (1 pM-1 μM), a low detection limit of 0.2710 pM, a quantitation limit of 0.9033 pM, reproducibility with an RSD value of 1.26 %, and stable storage for at least one week. The RSD value of CYP2C19*3 in serum samples consistently remained below 4.5 %, with a recovery rate ranging 95.52 % from 102.71 %. Moreover, the target gene could be accurately identified and captured in a mixed system of multiple nucleotide mutants of the CYP2C19*3 gene, suggesting a promising applicability and popularization.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"160 ","pages":"Article 108792"},"PeriodicalIF":4.8,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141910911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial for the special issue of bioelectrochemistry: Diagnostic biosensors for infectious diseases 生物电化学特刊编辑：传染病诊断生物传感器。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-05 DOI: 10.1016/j.bioelechem.2024.108790

Eithne Dempsey, Baljit Singh

引用次数: 0

A photoelectrochemical sensor based on In2S3/AgInS2 in situ Z-type heterojunction with “photo-modulated interface charge” for sensitive detection of Programmed Death-Ligand 1 基于 In2S3/AgInS2 原位 Z 型异质结的光电化学传感器，具有 "光调制界面电荷 "功能，可灵敏检测程序性死亡配体 1。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-04 DOI: 10.1016/j.bioelechem.2024.108791

Ming Cao , Hui Zhou , Xiaoran Wu , Xiaoxia Chen , Xiaomin Ren , Linlin Cao , Yueyuan Li , Shujun Wang , Yueyun Li , Qing Liu

{"title":"A photoelectrochemical sensor based on In2S3/AgInS2 in situ Z-type heterojunction with “photo-modulated interface charge” for sensitive detection of Programmed Death-Ligand 1","authors":"Ming Cao , Hui Zhou , Xiaoran Wu , Xiaoxia Chen , Xiaomin Ren , Linlin Cao , Yueyuan Li , Shujun Wang , Yueyun Li , Qing Liu","doi":"10.1016/j.bioelechem.2024.108791","DOIUrl":"10.1016/j.bioelechem.2024.108791","url":null,"abstract":"<div><p>The construction of heterostructure photoelectrodes can enhance the performance of photoelectrochemical (PEC) sensors. However, it is still a critical challenge to achieve efficient transfer of interface carriers. In this paper, we propose a strategy of “photo-modulated interface charge” to design a PEC sensor based on a hollow hexagonal tubular In<sub>2</sub>S<sub>3</sub>/AgInS<sub>2</sub> in situ Z-type heterojunction for the susceptible detection of Programmed Death-ligand 1 (PD-L1). The hollow structured In<sub>2</sub>S<sub>3</sub>/AgInS<sub>2</sub> is ingeniously synthesized employing indium-sourced MIL-68 as a sacrificial template and in situ cation exchange technique. This composite material has close contact interfaces due to in situ growth, which facilitates the spontaneous establishment of a robust and stable built-in electric field between the interfaces. Moreover, the inner cavity structure promotes multiple light refractions and scatterings, significantly enhancing light trapping capability. Under the influence of both light irradiation and electric field force, the migration direction of the interfacial charge is reversed, forming a Z-transfer path, which effectively delays the compounding of the electron-hole pairs (e<sup>-</sup>/h<sup>+</sup>) and further improves the sensitivity of the sensor. The minimum detection threshold of the PEC sensor is 26.58 fg/mL, and the feasibility of real samples is investigated, providing new insights for early diagnosis and prognostic treatment of diseases.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"160 ","pages":"Article 108791"},"PeriodicalIF":4.8,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel sandwich electrochemical immunosensor utilizing customized template and phosphotungstate catalytic amplification for CD44 detection 利用定制模板和磷钨酸催化放大技术检测 CD44 的新型夹心电化学免疫传感器。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-02 DOI: 10.1016/j.bioelechem.2024.108787

Ping Xia, Yuchun Fu, Qian Chen, Lianhai Shan, Chungu Zhang, Shun Feng

{"title":"A novel sandwich electrochemical immunosensor utilizing customized template and phosphotungstate catalytic amplification for CD44 detection","authors":"Ping Xia, Yuchun Fu, Qian Chen, Lianhai Shan, Chungu Zhang, Shun Feng","doi":"10.1016/j.bioelechem.2024.108787","DOIUrl":"10.1016/j.bioelechem.2024.108787","url":null,"abstract":"<div><p>A sandwich-type electrochemical immunosensor was proposed for the ultra-sensitive detection of CD44, a potential biomarker for breast cancer. In this design, a customized template-based ionic liquid (1-butyl-2,3-dimethylimidazolium tetrafluoroborate) carbon paste electrode (CILE) served as the sensing platform, and thionine/Au nanoparticles/covalent-organic frameworks (THI/Au/COF) were used as the signal label. Moreover, an enzyme-free signal amplification strategy was introduced by involving H<sub>2</sub>O<sub>2</sub> and phosphotungstate (PW<sub>12</sub>) with peroxidase-like activity. Under optimized conditions, the linear range is as wide as six orders of magnitude, and the detection limit is as low as 0.71 pg mL<sup>−1</sup> (estimated based on S/N = 3). Average recoveries range from 98.16 %-100.1 %, with a relative standard deviation (RSD) of 1.42–8.27 % in mouse serum, and from 98.44 %-99.06 %, with an RSD of 1.14–4.84 % (n = 3) in artificial saliva. Furthermore, the immunosensor exhibits excellent specificity toward CD44, good stability, and low cost, indicating great potential for application in clinical trials.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"160 ","pages":"Article 108787"},"PeriodicalIF":4.8,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141887778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0