Bioelectrochemistry最新文献

{"title":"Carbon nanotubes/polythiophene composite anodes: Fabrication and electrochemical energy storage performance","authors":"Yuyang Wang ,&nbsp;Xiangquan Kong ,&nbsp;Zhijie Wang ,&nbsp;Jinlong Zuo ,&nbsp;Dongming Zhang ,&nbsp;Su Ma ,&nbsp;Yu Song ,&nbsp;Yuchu Chen ,&nbsp;Guofeng Duan","doi":"10.1016/j.bioelechem.2025.109167","DOIUrl":"10.1016/j.bioelechem.2025.109167","url":null,"abstract":"<div><div>This study presents a significant advancement in a capacitive carbon felt/carbon nanotube/polythiophene (CF/CNT/PTh) bioanode. The CF framework provides mechanical stability. CNTs form a mesoporous structure (Barrett-Joyner-Halenda (BJH) pore volume: 0.016991 cm³/g), which supports increased populations of electroactive microorganisms. High-throughput sequencing confirmed a 6.67-fold increase in the relative abundance of electroactive genera. The key innovation of the CF/CNT/PTh is the incorporation of PTh to redesign the bioelectrochemical interface. The hydrophobic surface of PTh (confirmed by Fourier-transform infrared spectroscopy and Brunauer-Emmett-Teller analysis) reduces interfacial water barriers. Consequently, the charge-transfer resistance decreases by 65.3 % (R_ct = 2.80 Ω), as measured via electrochemical impedance spectroscopy. Additionally, the pseudocapacitive properties of PTh enable the storage of 5842.55C/m² of charge and generate a stronger bioelectric field (−578 mV open-circuit potential (OCP)), which enhances microbial activity. These effects create an “energy hub” in the bioanode, as stored and real-time electrons merge during discharge. Therefore, the CF/CNT/PTh bioanode achieves a current density of 147.925 A/m² and a power density of 1216.03 mW/m², which is 1.64 times that of bare CF. This design establishes a novel system through a microbial habitat environment constructed with CNT and a polythiophene-enhanced electron transfer mechanism, offering an innovative solution for micropower applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109167"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145511401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical immunosensor based on sea urchin-like PdAg and Au NPs/N-C@CNTs for ultrasensitive detection of CEA","authors":"Yang Li ,&nbsp;Hongzhu Yan ,&nbsp;Zhe Zhao,&nbsp;Ping Wang,&nbsp;Feng Tang,&nbsp;Qingyan Chu,&nbsp;Zhao Yang,&nbsp;Shujun Wang,&nbsp;Qing Liu,&nbsp;Yueyun Li","doi":"10.1016/j.bioelechem.2025.109124","DOIUrl":"10.1016/j.bioelechem.2025.109124","url":null,"abstract":"<div><div>Carcinoembryonic antigen (CEA), a clinically critical tumor biomarker, enables early cancer screening and diagnosis. Here, we describe a sandwich-structured electrochemical immunosensing platform enabling supersensitive CEA quantification, leveraging synergistic signal amplification by sea urchin-like PdAg nanostructures and Au NPs/N-C@CNTs substrates. The urchin-like morphology of PdAg endows the material additional catalytic active sites for hydrogen peroxide reduction, which has remarkable electrochemical performance. Moreover, PdAg with superior biocompatibility can effectively immobilize the secondary antibody. Polydopamine-coated carbon nanotubes are carbonized to yield nitrogen-doped carbon nanotubes (N-C@CNTs), which are bound to gold nanoparticles (Au NPs) via stable Au<img>N bonds, thereby facilitating the subsequent binding of primary antibodies to the Au NPs. Optimized assays demonstrated a broad dynamic range (50 fg mL⁻¹–100 ng mL⁻¹) with low detection limits (1.04 fg mL⁻¹, S/N = 3), coupled with exceptional reproducibility, selectivity, and stability. This platform holds significant promise for the screening of early-stage tumor biomarkers.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109124"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145197704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive study of nitroxoline – Ct-DNA interaction using electrochemical, spectroscopic, viscometry and thermodynamics as analytical tools","authors":"Umbreen Ashraf ,&nbsp;Iqra Saleem ,&nbsp;Athar Yaseen Khan ,&nbsp;Mariya al Rashida ,&nbsp;Safeer Ahmad","doi":"10.1016/j.bioelechem.2025.109163","DOIUrl":"10.1016/j.bioelechem.2025.109163","url":null,"abstract":"<div><div>Nitroxoline (NXN) is an antibiotic of nitroquinoline family which is used for the treatment of urinary tract infections and as a biofilm eradicating agent. Since it shows potential antitumor activity, it is also considered a promising candidate for repurposing in cancer treatment. Present article reports on electrochemical investigations of nitroxoline, its interaction with ct-DNA using cyclic voltammetry, UV–visible spectroscopy, viscometry, molecular modelling, and thermodynamics as analytical tools. An irreversible reduction peak is observed at about −0.45 V at pH 4.0 which shifts to more negative potentials with increasing scan rate. With the addition of DNA, the signal intensity decreases indicating formation of an adduct which enabled calculation of binding constant <span><math><msub><mi>K</mi><mi>b</mi></msub></math></span> = (9.175<span><math><mo>±</mo></math></span>0.728) × 10⁴ M⁻¹ and 2S = (0.925<span><math><mo>±</mo></math></span>0.150). Spectroscopic measurements yielded a value of (3.366<span><math><mo>±</mo></math></span>0.0.193) × 10⁴ M⁻¹. Viscosity measurements show intercalation binding mode for the drug which is supported by preliminary molecular docking studies. Thermodynamic studies reveal that ∆G° is negative and both ∆H° and ∆S° are positive, indicating spontaneity of the binding process and hydrophobic forces are dominant in binding of the drug. Electrochemical parameters, transfer coefficient (<span><math><mi>α</mi></math></span>), diffusion coefficient (D₀) and heterogeneous electron transfer rate (<span><math><msub><mi>k</mi><mrow><mi>s</mi><mo>,</mo><mi>h</mi></mrow></msub><mo>)</mo></math></span> obtained for nitroxoline at pH 4.0 indicate mild electron transfer kinetics.</div><div>Quinoline structure and nitro group are medicinally important. Present study reports for the first time qualitative and quantitative data on an important member of the quinoline family which would be of interest to researchers engaged in drug development.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109163"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145562064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of peroxidase-modified zeolite carbon paste electrodes (Prx-Zeo/CPE) for the biosensing of hydroquinone in pharmaceutical skin cream","authors":"Roberta C. Martins ,&nbsp;Pedro A. da Silva Pereira ,&nbsp;Alexandre L.B. Baccaro ,&nbsp;Lúcio Angnes ,&nbsp;Adelir A. Saczk ,&nbsp;Fabiana S. Felix","doi":"10.1016/j.bioelechem.2025.109150","DOIUrl":"10.1016/j.bioelechem.2025.109150","url":null,"abstract":"<div><div>Peroxidases are oxidoreductase enzymes with intense catalytic activity toward the peroxidation of hydrogen donors in the presence of hydrogen peroxide. These enzymes are appealing for application as the biomolecular recognition step of electrochemical biosensors, since they can be easily extracted from vegetable tissues such as <em>Solanum aethiopicum</em> (popularly known as “Gilo”), considerably reducing the costs of sensing devices. Meanwhile, zeolites are hydrated aluminosilicates arranged in 3D tetrahedral crystalline reticules that form microporous inner structures with high internal and external surface areas, very convenient for the immobilization of high amounts of enzymes to possibly improve the electrochemical biosensors sensitivity. In this study, a peroxidase biosensor utilizing enzymes extracted from raw Gilo tissue was developed. The high adsorption capacity of zeolites (MFI-based NaY) was explored to immobilize the peroxidase enzymes. The extraction efficiency was evaluated using three different types of polymeric protectors: polyvinylpyrrolidone (PVP K90), latex, or polyvinyl alcohol (PVOH. Among them, PVOH was identified as the most effective protector. The peroxidase enzyme was immobilized on NaY zeolite, forming the peroxidase-modified zeolite that was added to carbon paste electrodes to assemble the <em>Prx</em>-Zeo/CPE bioelectrode. The effects of graphite powder, zeolite, mineral oil, and peroxidase concentration in the paste composition were investigated using factorial planning with the premises of the Design of Experiments (DOE) to optimize cathodic current values for hydroquinone detection. Finally, the <em>Prx</em>-Zeo/CPE biosensor was applied to determine the hydroquinone content in two different of skin cream samples. Hydroquinone were determined by direct interpolation of the sample analytical signals onto an analytical curve (0.2–1.0 mmol L⁻¹) and subsequently compared with the label nominal values provided by the manufacturers, as well as with those obtained through parallel analysis using an HPLC reference method.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109150"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145385542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ratiometric electrochemical sensor based on metal-organic framework and nanosilver as response signals for cTnI detection","authors":"Yanju Liu ,&nbsp;Gao Si ,&nbsp;Yuning Zhao ,&nbsp;Zhixiang Liu ,&nbsp;Xiaohua He ,&nbsp;Huaixia Yang ,&nbsp;Liying Zhao","doi":"10.1016/j.bioelechem.2025.109099","DOIUrl":"10.1016/j.bioelechem.2025.109099","url":null,"abstract":"<div><div>The concentration of cardiac troponin I (cTnI) serves as a well-established biomarker of myocardial injury. In this work, a ratiometric electrochemical aptasensor utilizing Zirconium-metal organic framework (UiO-66) and AgNPs as electrochemical signaling tags were developed for the ultrasensitive detection of cardiac troponin I (cTnI). Upon specific binding between cTnI and the aptamer, probe 1 (P1) dissociated from the aptamer, resulting in a decreased UiO-66 signal. Meanwhile, signal amplification mediated by terminal deoxynucleotide transferase (TdT) provided a substantial quantity of active sites for surface-initiated reversible addition fragmentation chain transfer (SI-RAFT) polymerization, leading to a massive amount of silver nano-particles deposited on the electrode surfaces with enhanced silver ion signals. The concentration range of cTnI was determined to be 1 × 10⁻³–1 × 10² ng mL⁻¹, with a detection limit as low as 20.53 fg mL⁻¹. Notably, the ratiometric sensor performed well in detecting cTnI in serum samples from patients, indicating clinical application potential. Importantly, the aptasensor exhibited excellent performance in detecting cTnI in human serum samples, highlighting its strong potential for clinical application.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109099"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly water-retaining conductive hydrogels based on multi-crosslinked networks for ultrasensitive sensing platform","authors":"Jiaqing Wang ,&nbsp;Youyu Li ,&nbsp;Ning Li ,&nbsp;Hongliang Han ,&nbsp;Zhanfang Ma ,&nbsp;Haijun Yang","doi":"10.1016/j.bioelechem.2025.109120","DOIUrl":"10.1016/j.bioelechem.2025.109120","url":null,"abstract":"<div><div>Hydrogels are widely used in electrochemical sensors due to their unique properties, but their conductivity, influenced by water-content, is highly susceptible to external environment. Therefore, enhancing the water-retention of hydrogels while ensuring stable conductivity and analytical performance is crucial for broadening their application. In this work, a novel polyacrylamide/bacterial cellulose/sulfobetaine methacrylate/sodium alginate composite hydrogel (PBSS)-based multi-crosslinked network hydrogel was designed. The water retention of the PBSS hydrogel was improved by a factor of 1.5 compared to the unreinforced polyacrylamide (PAM) hydrogel (Water loss of hydrogel exposed for 12 h at 37 °C). With the water retention properties of itself, the PBSS hydrogel retained 86 % of its initial conductivity after 12 h of exposure at 60 °C, whereas the PAM hydrogel not only exhibited poor initial conductivity but also lost up to 47 % of its conductivity. PBSS hydrogels were designed as sensing platforms and CaCO₃ spheres were designed as immunoprobes. Ca²⁺ released by the probe rivals Ni²⁺ for the signaling substance on the substrate, enabling the quantification of the target analyte. The sensor exhibited excellent analytical performance and maintained stable performance after four days of storage at 37 °C, offering a promising approach to enhance hydrogel sensor stability for clinical applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109120"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of initial cell counts on the microbiologically influenced corrosion of L245N steel in shale gas environments","authors":"Yanran Wang ,&nbsp;Hongfa Huang ,&nbsp;Yongfan Tang ,&nbsp;Shaomu Wen ,&nbsp;Xulin Hou","doi":"10.1016/j.bioelechem.2025.109157","DOIUrl":"10.1016/j.bioelechem.2025.109157","url":null,"abstract":"<div><div>Microbiologically influenced corrosion (MIC) caused by sulfate-reducing bacteria (SRB) poses a major threat to pipeline integrity in shale gas operations. Current industry standards set a control threshold of 25 cells/mL for SRB, but its scientific validity remains unclear. This study investigates the corrosion behavior of L245N steel under varying initial SRB concentrations (10⁴ to 25 cells/mL) with 50 ppm THPS biocide presence. Results reveal a distinct threshold effect between 10³ and 10² cells/mL, where corrosion severity, biofilm thickness, and sessile cell count drop sharply. Further reductions below 10² cells/mL produce negligible corrosion impact. However, there is no substantial difference between 100, 50, and 25 cells/mL, indicating a plateau effect. Sessile cell counts, biofilm morphology, electrochemical parameters, and corrosion rates all support this finding. Additionally, mixed microbial consortia enhanced SRB survival under biocidal conditions. These results suggest that the current standard may not be sufficient for MIC prevention and highlight the need for revised standards based on corrosion behavior rather than planktonic cell counts alone.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109157"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145547485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electron transfer performance and mechanism in twin microbial fuel cell powered electro-Fenton system with waste activated sludge as substrate","authors":"Jiaqi Lv ,&nbsp;Qingliang Zhao ,&nbsp;Junqiu Jiang ,&nbsp;Jing Ding ,&nbsp;Liangliang Wei ,&nbsp;Jiawen Liang","doi":"10.1016/j.bioelechem.2025.109154","DOIUrl":"10.1016/j.bioelechem.2025.109154","url":null,"abstract":"<div><div>The twin microbial fuel cell powered electro-Fenton system (twin-MFCⓅEFs), combining active oxygen component and microbial metabolism, was constructed to improve the treatment process of waste activated sludge (WAS). Nevertheless, the performance and mechanism of electron transfer underlying this enhancement remain poorly understood. This study investigated the performance and mechanism of electron generation and utilization in twin-MFCⓅEFs with WAS as substrate. The higher electron generation and recovery efficiency (8.25 % of coulombic efficiency) was attributed to the higher content of amino acids (such as tryptophan), humic substances and their aromatic groups and unsaturated conjugated double bonds in the soluble organic matter, which facilitated biodegradation and electron transfer. The higher electron utilization performance (52.76 % of faraday efficiency) relied on the superior electron supply system that exhibited greater free radical oxidation. Metagenomic analysis indicated that an increased secretory capacity of glycosyltransferases (including glucosyltransferases and β-glucosidases) and a reduced activity of acetate kinase and methyl-coenzyme M reductase alpha subunit in cellular metabolic processes favored signaling and electricity production. The study focused on electron flow in twin-MFCⓅEFs and offered a promising strategy for improving the sludge treatment process.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109154"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145385494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroactive TiO₂–Cu nanotube platform with synergistic charge modulation and Cu2+ release for marine antifouling","authors":"Yuqiao Dong ,&nbsp;Yuxuan Xu ,&nbsp;Jinke Yin ,&nbsp;Qianwen Jin ,&nbsp;Guangzhou Liu","doi":"10.1016/j.bioelechem.2025.109135","DOIUrl":"10.1016/j.bioelechem.2025.109135","url":null,"abstract":"<div><div>Biological damage remains a critical limiting factor that hinders the application of highly biocompatible titanium alloys in marine engineering. Exploiting the delicate electrostatic interactions at the interface between organisms and nanomaterials is of vital importance. In this study, an external direct current was applied to copper-coated capacitive TiO₂ nanotubes (TNT-Cu) to evaluate the antifouling effect on sessile biofilms and planktonic bacteria, and to elucidate the underlying mechanisms. The heterostructure exhibited high specific capacitance and superior antifouling performance. Upon electrochemical charge-discharge, TNT-Cu achieved 98.3 ± 0.9 % adhesion inhibition and 96.0 ± 2.0 % algicidal activity against <em>Phaeodactylum tricornutum</em>. The charged TNT-Cu achieved synergistic antifouling through surface charge–induced electrostatic sterilization and controlled Cu²⁺ release. Physiologically, the electrical interaction combined with Cu²⁺ significantly disrupted algal electron transport, induced reactive oxygen species (ROS) accumulation, and caused membrane rupture. This work provides a promising, durable, and eco-friendly antifouling strategy for marine applications of titanium-based materials.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109135"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145297905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive electrochemiluminescence immunosensor based on gold-functionalised NiFe layered double hydroxide and electro-negative mesoporous silica for prostate-specific antigen detection","authors":"Dongcheng Yang ,&nbsp;Huan Wang ,&nbsp;Caiyu Wang ,&nbsp;Xinyu Liu ,&nbsp;Lihua Hu ,&nbsp;Hongmin Ma ,&nbsp;Dan Wu ,&nbsp;Xiang Ren ,&nbsp;Yuyang Li ,&nbsp;Qin Wei","doi":"10.1016/j.bioelechem.2025.109145","DOIUrl":"10.1016/j.bioelechem.2025.109145","url":null,"abstract":"<div><div>Electrochemiluminescence (ECL) technology has become an essential analytical methodology in biomolecular detection, attaining both profound research advancements and extensive practical applications in this domain. Herein, we used carboxylated mesoporous silica (MSN-COOH) as a container to encapsulate Tris(2,2-bipyridyl)ruthenium(II)²⁺ (Ru(bpy)₃²⁺)—a luminophore—in its pores to achieve enrichment (RuMSN). The reactive intermediates Ru(bpy)₃³⁺ and TPrA^•+ are attracted by the electro-negative carboxyl group on the MSN-COOH surface, substantially reducing the reaction distance and improving the ECL response efficiency. The flower-like Ni<img>Fe layered double hydroxide (NiFe-LDH) nanostructure possesses significantly high specific surface area and good electrocatalytic performance, while gold nanoparticles also demonstrate excellent electrical conductivity and biocompatibility. To enhance sensor sensitivity, gold nanoparticles were loaded onto the NiFe-LDH surface forming the Au@NiFe-LDH composite, which improves performance by enhancing conductive properties and increasing antigen-antibody binding sites. This study established a sandwich-configuration electrochemiluminescence detection platform capable of highly sensitive PSA analysis.The biosensor demonstrated excellent stability, specificity and selectivity, with a linear detection range of 0.1 pg/mL-50 ng/mL and a detection limit as low as 63 fg/mL (S/N = 3). This confirms the clinical application value of the detection system in the early screening of prostate cancer.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109145"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145342478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}