Bioelectrochemistry最新文献_第9页

Spectroelectrochemical studies of TDMQ20: A potential drug against Alzheimer’s disease TDMQ20 的光谱电化学研究：治疗阿尔茨海默病的潜在药物

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-10 DOI: 10.1016/j.bioelechem.2024.108814

Magdalena Z. Wiloch , Martin Perez-Estebanez , Natalia Baran , Aranzazu Heras , Martin Jönsson-Niedziółka , Alvaro Colina

{"title":"Spectroelectrochemical studies of TDMQ20: A potential drug against Alzheimer’s disease","authors":"Magdalena Z. Wiloch , Martin Perez-Estebanez , Natalia Baran , Aranzazu Heras , Martin Jönsson-Niedziółka , Alvaro Colina","doi":"10.1016/j.bioelechem.2024.108814","DOIUrl":"10.1016/j.bioelechem.2024.108814","url":null,"abstract":"<div><p>Alzheimer’s Disease (AD), reported for the first time in 1906, is a common disease that remains incurable to this day. In the past, a family of treatments using Cu(II) chelators failed during clinical trials, evidencing the importance of pre-clinical studies. In this work, we performed electrochemical characterisation of TDMQ20, a new potential drug against AD, using electrochemistry and spectroelectrochemistry. On the basis of voltammetry, we determined that TDMQ20 undergoes a two-step irreversible oxidation process and a one-step irreversible reduction process. Both oxidation and reduction reactions are pH-sensitive. Bidimensional UV–Vis spectroelectrochemistry (UV–Vis-SEC) allowed us to confirm that oxidation of TDMQ20 can occur both on the aliphatic chain and on the aromatic ring. The results expand the knowledge of the TDMQ20 redox activity in the human body which is important from the point of view of the toxicity of the proposed therapy.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108814"},"PeriodicalIF":4.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001762/pdfft?md5=9d4fab10a776b19906ab440a3eeea508&pid=1-s2.0-S1567539424001762-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous detection of ovarian cancer related miRNA biomarkers with carboxylated graphene oxide modified electrochemical biosensor platform 利用羧基氧化石墨烯修饰的电化学生物传感器平台同时检测卵巢癌相关 miRNA 生物标记物

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-05 DOI: 10.1016/j.bioelechem.2024.108806

Ezgi Kivrak , Pinar Kara

{"title":"Simultaneous detection of ovarian cancer related miRNA biomarkers with carboxylated graphene oxide modified electrochemical biosensor platform","authors":"Ezgi Kivrak , Pinar Kara","doi":"10.1016/j.bioelechem.2024.108806","DOIUrl":"10.1016/j.bioelechem.2024.108806","url":null,"abstract":"<div><p>Ovarian cancer, known as “silent killer”, is a gynocological cancer with high mortality that usually diagnosed in the late stages. Gold standard immunoassay technique is evaluation of CA-125 levels which is not merely specific to ovarian cancer. Therefore, there is a need for sensitive determination of more specific biomarkers. miR-200 family is RNA nucleic acids that known to be upregulated in the presence of ovarian cancer. Since diagnosis based on a single biomarker is prone to generate misleading results, it is important to develop point-of-care systems that allow diagnosis of multiple miRNAs. Herein, an electrochemical nanobiosensor platform was developed for the multiplexed and simultaneous detection of miR-200c and miR-141. Biorecognition part was constitutued of methylene blue and ferrocene labeled hairpin DNA probes immobilized onto carboxylated graphene oxide modified pencil graphite electrodes. Their hybridization with miRNAs were examined upon “signal-off” approach using Square Wave Voltammetry. The platform demonstrated a linear detection range of 0.1 pM to 10 nM for both miR-141 and miR-200c, with low detection limits of 0.029 pM and 0.026 pM, respectively. We assume that the developed biosensor platform may pave the way in early diagnosis of the disease and the development of more effective treatment strategies.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108806"},"PeriodicalIF":4.8,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001683/pdfft?md5=dbaeb4cee74d650bfb7e250f9124b7ae&pid=1-s2.0-S1567539424001683-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A biomimetic skin microtissue biosensor for the detection of fish parvalbumin 用于检测鱼类副缬氨酸的仿生皮肤微组织生物传感器

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-03 DOI: 10.1016/j.bioelechem.2024.108805

Donglei Jiang , Yang Xu , Hui Jiang , Xinyue Xiang , Lifeng Wang

{"title":"A biomimetic skin microtissue biosensor for the detection of fish parvalbumin","authors":"Donglei Jiang , Yang Xu , Hui Jiang , Xinyue Xiang , Lifeng Wang","doi":"10.1016/j.bioelechem.2024.108805","DOIUrl":"10.1016/j.bioelechem.2024.108805","url":null,"abstract":"<div><p>In this paper, a biomimetic skin microtissue biosensor was developed based on three-dimensional (3D) bioprinting to precisely and accurately determine fish parvalbumin (FV). Based on the principle that allergens stimulate cells to produce ONOO<sup>−</sup> (peroxynitrite anion), a screen-printed electrode for the detection nanomolar level ONOO<sup>−</sup> was innovatively prepared to indirectly detect FV based on the level of ONOO<sup>−</sup> release. Gelatin methacryloyl (GelMA), RBL-2H3 cells, and MS1 cells were used as bio-ink for 3D bioprinting. The high-throughput and standardized preparation of skin microtissue was achieved using stereolithography 3D bioprinting technology. The printed skin microtissues were put into the self-designed 3D platform that integrated cell culture and electrochemical detection. The experimental results showed that the sensor could effectively detect FV when the optimized ratio of RBL-2H3 to MS1 cells and allergen stimulation time were 2:8 and 2 h, respectively. The linear detection range was 0.125–3.0 μg/mL, and the calculated lowest detection limit was 0.122 μg/mL. In addition, the sensor had excellent selectivity, specificity, stability, and reliability. Thus, this study successfully constructed a biomimetic skin microtissue electrochemical sensor for PV detection.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108805"},"PeriodicalIF":4.8,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001671/pdfft?md5=ee7d1ef56d00f04b242a7eb9667b42de&pid=1-s2.0-S1567539424001671-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142169544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the electrochemical performance of glassy carbon electrodes by surface engineered, sustainable chitosan membranes 通过表面工程化的可持续壳聚糖膜释放玻璃碳电极的电化学性能

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-03 DOI: 10.1016/j.bioelechem.2024.108804

Agata Smułka , Mateusz Cieślik , Adrian Olejnik , Artur Zieliński , Jacek Ryl , Tadeusz Ossowski

{"title":"Unlocking the electrochemical performance of glassy carbon electrodes by surface engineered, sustainable chitosan membranes","authors":"Agata Smułka , Mateusz Cieślik , Adrian Olejnik , Artur Zieliński , Jacek Ryl , Tadeusz Ossowski","doi":"10.1016/j.bioelechem.2024.108804","DOIUrl":"10.1016/j.bioelechem.2024.108804","url":null,"abstract":"<div><p>Chitosan coatings, derived from crustacean shell waste, possess inherent biocompatibility and biodegradability, rendering them suitable for various biomedical and environmental applications, including electrochemical biosensing. Its amine and hydroxyl functional groups offer abundant sites for chemical modifications to boost the charge transfer kinetics and provide excellent adhesion, enabling the construction of robust electrode-coating interfaces for electroanalysis. This study explores the role of electrostatically-driven chemical interactions and crosslinking density originating from different chitosan (Cs) and glutaraldehyde (Ga) concentrations in this aspect. Studying anionic ([Fe(CN)<sub>6</sub>]<sup>3−/4−</sup>), neutral (FcDM<sup>0/+</sup>), and cationic ([Ru(NH<sub>3</sub>)<sub>6</sub>]<sup>2+/3+</sup>) redox probes highlights the influence of Coulombic interactions with chitosan chains containing positively-charged pathways, calculated by DFT analysis. Our study reveals how a proper Ch-to-Ga ratio has a superior influence on the cross-linking efficacy and resultant charge transfer kinetics, which is primarily boosted by up to 20× analyte preconcentration increase, due to electrostatically-driven migration of negatively charged ferrocyanide ions toward positively charged chitosan hydrogel. Notably the surface engineering approach allows for a two-orders of magnitude enhancement in [Fe(CN)<sub>6</sub>]<sup>4−</sup> limit of detection, from 0.1 µM for bare GCE down to even 0.2 nM upon an adequate hydrogel modification.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108804"},"PeriodicalIF":4.8,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S156753942400166X/pdfft?md5=cb0b9c149ce27a020849947a9a8485dc&pid=1-s2.0-S156753942400166X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-tumor effects of nanosecond pulsed electric fields in a murine model of pancreatic cancer 纳秒脉冲电场在小鼠胰腺癌模型中的抗肿瘤作用

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-31 DOI: 10.1016/j.bioelechem.2024.108803

Yuan-yuan Liang , Zhou Lu , Hong-wei Liu , Qi Huang , Xue-ting Zheng , Xiao-an Li , Yan Zhou

{"title":"Anti-tumor effects of nanosecond pulsed electric fields in a murine model of pancreatic cancer","authors":"Yuan-yuan Liang , Zhou Lu , Hong-wei Liu , Qi Huang , Xue-ting Zheng , Xiao-an Li , Yan Zhou","doi":"10.1016/j.bioelechem.2024.108803","DOIUrl":"10.1016/j.bioelechem.2024.108803","url":null,"abstract":"<div><p>Nanosecond Pulsed Electric Fields (nsPEFs) treatment has demonstrated anti-tumor effects on various cancer cell lines. However, the use of this treatment in pancreatic cancer is limited. This study demonstrated that nsPEFs treatment effectively suppressed the proliferation and metastasis of pancreatic cancer cells, while also inducing DNA damage. Meanwhile, animal experiments have shown that nsPEFs effectively suppressed the growth of pancreatic cancer, even in cases where the tumor volume exceeded 500–600 mm<sup>3</sup> at the initiation of treatment. Notably, a single treatment session was found to significantly inhibit tumor growth, while also showing no adverse effects on the main organs of the mice. RNA sequencing and bioinformatics revealed that seven key genes (CDK1, CENPA, UBE2C, CCNB2, PLK1, CCNA2, and CCNB14) were significantly correlated with the overall survival rate of patients with pancreatic cancer. Through the application of the competing endogenous RNA (ceRNA) hypothesis, two miRNAs (has-let-7b-5p and hsa-miR-193b-3p) and four lncRNAs (MIR4435-2HG, ZNF436-AS1, LINC01089, and MIR4435-2HG) were identified as significantly impacting the overall survival of pancreatic cancer patients. We have effectively developed an mRNA-miRNA-lncRNA network that has the potential to stimulate further investigation into the underlying mechanisms of nsPEFs on pancreatic cancer.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108803"},"PeriodicalIF":4.8,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001658/pdfft?md5=c51fee14194bf482031ed83387989340&pid=1-s2.0-S1567539424001658-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142144533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective detection of alpha synuclein amyloid fibrils by faradaic and non-faradaic electrochemical impedance spectroscopic approaches 通过法拉第和非法拉第电化学阻抗光谱法选择性检测α-突触核蛋白淀粉样纤维。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-30 DOI: 10.1016/j.bioelechem.2024.108800

Hussaini Adam , Subash C.B. Gopinath , Hemavathi Krishnan , Tijjani Adam , Makram A. Fakhri , Evan T. Salim , A. Shamsher , Sreeramanan Subramaniam , Yeng Chen

{"title":"Selective detection of alpha synuclein amyloid fibrils by faradaic and non-faradaic electrochemical impedance spectroscopic approaches","authors":"Hussaini Adam , Subash C.B. Gopinath , Hemavathi Krishnan , Tijjani Adam , Makram A. Fakhri , Evan T. Salim , A. Shamsher , Sreeramanan Subramaniam , Yeng Chen","doi":"10.1016/j.bioelechem.2024.108800","DOIUrl":"10.1016/j.bioelechem.2024.108800","url":null,"abstract":"<div><p>This study utilized faradaic and non-faradaic electrochemical impedance spectroscopy to detect alpha synuclein amyloid fibrils on gold interdigitated tetraelectrodes (AuIDTE), providing valuable insights into electrochemical reactions for clinical use. AuIDE was purchased, modified with zinc oxide for increased hydrophobicity. Functionalization was conducted with hexacyanidoferrate and carbonyldiimidazole. Faradaic electrochemical impedance spectroscopy has been extensively explored in clinical diagnostics and biomedical research, providing information on the performance and stability of electrochemical biosensors. This understanding can help develop more sensitive, selective, and reliable biosensing platforms for the detection of clinically relevant analytes like biomarkers, proteins, and nucleic acids. Non-faradaic electrochemical impedance spectroscopy measures the interfacial capacitance at the electrode–electrolyte interface, eliminating the need for redox-active species and simplifying experimental setups. It has practical implications in clinical settings, like real-time detection and monitoring of biomolecules and biomarkers by tracking changes in interfacial capacitance. The limit of detection (LOD) for normal alpha synuclein in faradaic mode is 2.39-fM, The LOD for aggregated alpha synuclein detection is 1.82-fM. The LOD for non-faradaic detection of normal alpha synuclein is 2.22-fM, and the LOD for nonfaradaic detection of aggregated alpha synuclein is 2.40-fM. The proposed EIS-based AuIDTEs sensor detects alpha synuclein amyloid fibrils and it is highly sensitive.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108800"},"PeriodicalIF":4.8,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001622/pdfft?md5=e4f03ec66ff269a51b62c9da774da92e&pid=1-s2.0-S1567539424001622-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142144535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term changes in transmembrane voltage after electroporation are governed by the interplay between nonselective leak current and ion channel activation 电穿孔后跨膜电压的长期变化受非选择性泄漏电流和离子通道激活之间相互作用的影响。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-30 DOI: 10.1016/j.bioelechem.2024.108802

Anja Blažič , Manon Guinard , Tomaž Leskovar , Rodney P. O’Connor , Lea Rems

{"title":"Long-term changes in transmembrane voltage after electroporation are governed by the interplay between nonselective leak current and ion channel activation","authors":"Anja Blažič , Manon Guinard , Tomaž Leskovar , Rodney P. O’Connor , Lea Rems","doi":"10.1016/j.bioelechem.2024.108802","DOIUrl":"10.1016/j.bioelechem.2024.108802","url":null,"abstract":"<div><p>Electroporation causes a temporal increase in cell membrane permeability and leads to prolonged changes in transmembrane voltage (TMV) in both excitable and non-excitable cells. However, the mechanisms of these TMV changes remain to be fully elucidated. To this end, we monitored TMV over 30 min after exposing two different cell lines to a single 100 µs electroporation pulse using the FLIPR Membrane Potential dye. In CHO-K1 cells, which express very low levels of endogenous ion channels, membrane depolarization following pulse exposure could be explained by nonselective leak current, which persists until the membrane reseals, enabling the cells to recover their resting TMV. In U-87 MG cells, which express many different ion channels, we unexpectedly observed membrane hyperpolarization following the initial depolarization phase, but only at 33 °C and not at 25 °C. We developed a theoretical model, supported by experiments with ion channel inhibitors, which indicated that hyperpolarization could largely be attributed to the activation of calcium-activated potassium channels. Ion channel activation, coupled with changes in TMV and intracellular calcium, participates in various physiological processes, including cell proliferation, differentiation, migration, and apoptosis. Therefore, our study suggests that ion channels could present a potential target for influencing the biological response after electroporation.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108802"},"PeriodicalIF":4.8,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001646/pdfft?md5=9b60750e97ae48ebdbe7c9b36e0ee95d&pid=1-s2.0-S1567539424001646-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142144534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disposable Zirconium trisulfide-Reduced graphene oxide modified conducting thread based electrochemical biosensor for lung cancer diagnosis 基于电化学生物传感器的一次性三硫化锆-还原氧化石墨烯修饰导电线用于肺癌诊断

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-23 DOI: 10.1016/j.bioelechem.2024.108801

Saurav Shankar , Yogesh Kumar , Neera Sharma , Ramesh Chandra , Suveen Kumar

{"title":"Disposable Zirconium trisulfide-Reduced graphene oxide modified conducting thread based electrochemical biosensor for lung cancer diagnosis","authors":"Saurav Shankar , Yogesh Kumar , Neera Sharma , Ramesh Chandra , Suveen Kumar","doi":"10.1016/j.bioelechem.2024.108801","DOIUrl":"10.1016/j.bioelechem.2024.108801","url":null,"abstract":"<div><p>Flexible technology in sensors have received much attention in monitoring of human health through various physiological indicators. Thus, it drawn a lot of interest in the development of flexible substrate for the diagnosis of various diseases via analysis of analytes. Present work focusses on the development of ecofriendly, portable, flexible, conducting thread (Th) and used as smart substrate for fabrication of biosensor towards ultrasensitive detection of the lung cancer biomarker (cytoskeleton-associated protein 4; CKAP4). The zirconium trisulfide-reduced graphene oxide nanocomposite and poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) modified cotton thread based biosensor was fabricated via dip coating method. Next, successive immobilization of monoclonal antibodies of CKAP4 (anti-CKAP4) and bovine serum albumin (BSA) was performed via drop cast approach using fabricated electrode [nZrS<sub>3</sub>@rGO/PEDOT:PSS/Th]. The response of fabricated electrode (BSA/anti-CKAP4/ZrS<sub>3</sub>@rGO/PEDOT:PSS/Th) was recorded electrochemically versus CKAP4 concentration via chronoamperometry (CA). The results showed wider linear detection range of 6.25-800 pg mL<sup>−1</sup>, excellent sensitivity of 85.2 µA[log(pg mL<sup>−1</sup>)]<sup>-1</sup>cm<sup>−2</sup> with good stability up to 42 days. The response of fabricated biosensor was supported by investigating response of CKAP4 biomarker present in patients of lung cancer (concentration as determined through enzyme-linked immunosorbent assay) and obtained results exhibited excellent correlation with that of standard samples.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"160 ","pages":"Article 108801"},"PeriodicalIF":4.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142122707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioimpedance formalism: A new approach for accessing the health status of cell and tissues 生物阻抗形式主义：获取细胞和组织健康状况的新方法

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-19 DOI: 10.1016/j.bioelechem.2024.108799

Jose Luis García Bello , Taira Batista Luna , Alcibíades Lara Lafargue , Héctor Manuel Camué Ciria , Yohandys A. Zulueta

引用次数: 0

Advancement in SARS-CoV-2 diagnosis: A new and stable electrochemical biosensor for genomic RNA detection SARS-CoV-2 诊断的进展：用于基因组 RNA 检测的新型稳定电化学生物传感器

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-08-17 DOI: 10.1016/j.bioelechem.2024.108798

José A.L. Gomide , Anna C.R. Moço , Leonardo T.R. Tanaka , Maikon R.A. Alexandre , Tárcio Peixoto Roca , Deusilene Souza Vieira Dall’acqua , Márcia M. Costa Nunes Soares , Ronaldo J. Oliveira , Renata C. Lima , João M. Madurro , Ana G. Brito-Madurro

{"title":"Advancement in SARS-CoV-2 diagnosis: A new and stable electrochemical biosensor for genomic RNA detection","authors":"José A.L. Gomide , Anna C.R. Moço , Leonardo T.R. Tanaka , Maikon R.A. Alexandre , Tárcio Peixoto Roca , Deusilene Souza Vieira Dall’acqua , Márcia M. Costa Nunes Soares , Ronaldo J. Oliveira , Renata C. Lima , João M. Madurro , Ana G. Brito-Madurro","doi":"10.1016/j.bioelechem.2024.108798","DOIUrl":"10.1016/j.bioelechem.2024.108798","url":null,"abstract":"<div><p>Coronavirus disease (COVID-19) is caused by infection with the SARS-CoV-2 virus, having already caused more than seven million deaths worldwide. Conventional techniques for SARS-CoV-2 detection have limitations, as high cost, low specificity, and longer analysis time, among others. Biosensors emerge as a necessary alternative to overcome the difficulties of current diagnostics. This paper reports a sensor platform where silver-doped zinc oxide nanomaterial (Ag:ZnONp) was used onto carbon screen-printed electrode and ethidium bromide as indicator for development of a specific electrochemical genosensor for COVID-19. This genosensor demonstrated good linearity between the concentrations of 5.62 × 10<sup>4</sup>–5.62 copies/mL and a detection limit of 5 copies/mL with gRNA in patient’s samples, with a response time within 30 min. Molecular modeling and morphological analysis are in agreement with obtained electrochemical results. Additional techniques such as AFM, SEM, and EIS were conducted to characterize the morphological and electrochemical properties of the biosensor’s surface. The biosensor was also capable of detecting the target presence in spiked samples and demonstrated a stability of 60 days, higher than other similar biosensors for SARS-CoV-2.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108798"},"PeriodicalIF":4.8,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001609/pdfft?md5=af107968f7f0e6beec9a622d6a7c4e48&pid=1-s2.0-S1567539424001609-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142144532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0