Modelling enzyme electrodes – What do we learn and how is it useful?

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-02-22 DOI:10.1016/j.bioelechem.2025.108941

Philip N. Bartlett, M. Hashim Khan

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Abstract

There has been an enormous increase in the computational power readily available since the first numerical treatments of electrochemical problems in the early 1960s. This development has been accompanied by the development of powerful, widely available, commercial software modelling tools. Despite this, approximate analytical treatments remain extremely useful in the modelling of coupled diffusion/reaction problems in electrochemistry because of the insights they provide into the different possible behaviours of the system. In this paper we discuss the modelling of amperometric enzyme electrodes, taking as our exemplar redox hydrogel-based enzyme electrodes in which the enzyme is immobilized in a redox active polymer which wires the enzyme to the electrode. In this system the measured current is related to many different experimental variables including substrate concentration and diffusion coefficient, reaction rate constants, and film properties and thickness. The interplay of these factors is described and the role of Case diagrams in understanding coupled diffusion/reaction problems of this type is discussed.

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酶电极建模-我们学到了什么？它如何有用？

自从20世纪60年代初首次对电化学问题进行数值处理以来，计算能力已经有了巨大的提高。这种发展伴随着强大的、广泛可用的商业软件建模工具的发展。尽管如此，近似分析处理在电化学中耦合扩散/反应问题的建模中仍然非常有用，因为它们提供了对系统不同可能行为的见解。本文讨论了电流型酶电极的建模，以酶固定在氧化还原活性聚合物中并将酶连接到电极上的氧化还原凝胶型酶电极为例。在该系统中，测量电流与许多不同的实验变量有关，包括衬底浓度和扩散系数，反应速率常数，薄膜性质和厚度。描述了这些因素的相互作用，并讨论了案例图在理解这类耦合扩散/反应问题中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.