Modelling enzyme electrodes – What do we learn and how is it useful?

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-02-22 DOI:10.1016/j.bioelechem.2025.108941

Philip N. Bartlett, M. Hashim Khan

{"title":"Modelling enzyme electrodes – What do we learn and how is it useful?","authors":"Philip N. Bartlett, M. Hashim Khan","doi":"10.1016/j.bioelechem.2025.108941","DOIUrl":null,"url":null,"abstract":"<div><div>There has been an enormous increase in the computational power readily available since the first numerical treatments of electrochemical problems in the early 1960s. This development has been accompanied by the development of powerful, widely available, commercial software modelling tools. Despite this, approximate analytical treatments remain extremely useful in the modelling of coupled diffusion/reaction problems in electrochemistry because of the insights they provide into the different possible behaviours of the system. In this paper we discuss the modelling of amperometric enzyme electrodes, taking as our exemplar redox hydrogel-based enzyme electrodes in which the enzyme is immobilized in a redox active polymer which wires the enzyme to the electrode. In this system the measured current is related to many different experimental variables including substrate concentration and diffusion coefficient, reaction rate constants, and film properties and thickness. The interplay of these factors is described and the role of Case diagrams in understanding coupled diffusion/reaction problems of this type is discussed.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"165 ","pages":"Article 108941"},"PeriodicalIF":4.8000,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioelectrochemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567539425000441","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

There has been an enormous increase in the computational power readily available since the first numerical treatments of electrochemical problems in the early 1960s. This development has been accompanied by the development of powerful, widely available, commercial software modelling tools. Despite this, approximate analytical treatments remain extremely useful in the modelling of coupled diffusion/reaction problems in electrochemistry because of the insights they provide into the different possible behaviours of the system. In this paper we discuss the modelling of amperometric enzyme electrodes, taking as our exemplar redox hydrogel-based enzyme electrodes in which the enzyme is immobilized in a redox active polymer which wires the enzyme to the electrode. In this system the measured current is related to many different experimental variables including substrate concentration and diffusion coefficient, reaction rate constants, and film properties and thickness. The interplay of these factors is described and the role of Case diagrams in understanding coupled diffusion/reaction problems of this type is discussed.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.