Bioelectrochemistry最新文献_第8页

Dual-mode detection of α-fetoprotein using the photothermal effect and peroxidase-like activity of Au@Cu/Cu2O-rGO 利用 Au@Cu/Cu2O-rGO 的光热效应和过氧化物酶样活性对α-胎儿蛋白进行双模式检测

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-21 DOI: 10.1016/j.bioelechem.2024.108822

Min Wang, Mingzhe Jiang, Wenjing Lai, Zihan Yan, Tingting Wang, Yu Qi, Chenglin Hong

引用次数: 0

Polynorepinephrine and polydopamine-bacterial laccase coatings for phenolic amperometric biosensors 用于酚类安培计生物传感器的聚肾上腺素和聚多巴胺-细菌漆酶涂层

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-20 DOI: 10.1016/j.bioelechem.2024.108826

Luís C. Almeida , Jorge F. Zeferino , Clara Branco , Guiseppe Squillaci , Alessandra Morana , Romana Santos , Petri Ihalainen , Liji Sobhana , Jorge P. Correia , Ana S. Viana

{"title":"Polynorepinephrine and polydopamine-bacterial laccase coatings for phenolic amperometric biosensors","authors":"Luís C. Almeida , Jorge F. Zeferino , Clara Branco , Guiseppe Squillaci , Alessandra Morana , Romana Santos , Petri Ihalainen , Liji Sobhana , Jorge P. Correia , Ana S. Viana","doi":"10.1016/j.bioelechem.2024.108826","DOIUrl":"10.1016/j.bioelechem.2024.108826","url":null,"abstract":"<div><div>The successful fabrication of biosensors is greatly limited by the immobilization of their bioreceptor, thus we propose a facile and reproducible two-step method to modify graphite electrodes with a bacterial laccase, relying on a fast and controllable potentiostatic process to coat graphite surfaces with biomolecule-compatible thin films of polynorepinephrine (ePNE) and polydopamine (ePDA). Both polymers, synthesized with a similar thickness, were functionalized with bacterial laccase, displaying distinct electrochemical transducing behaviours at pH 5.0 and 7.0. ePNE layer enables adequate electron transfer of anionic and cationic species in acidic and neutral media, whereas transduction across ePDA strongly depends on pH and redox probe charge. ePNE stands out by improving the amperometric responses of the biosensing interface towards a phenolic acid (gallic acid) and a flavonoid (catechin), in respect to ePDA. The optimal graphite/ePNE/laccase interface outperforms biosensing interfaces based on fungal laccases at neutral pH, displaying detection sensitivities of 104 and 14.4 µA cm<sup>−2</sup> mM<sup>−1</sup> <!-->for gallic acid and catechin, respectively. The fine synthetic control of the ePNE bio-inspired transduction layer and the use of an alkaliphilic bacterial laccase enabled the construction of an amperometric biosensing interface with extended pH range of polyphenols detection present in food products and agro-industrial waste.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108826"},"PeriodicalIF":4.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001889/pdfft?md5=862478700bbd86aee15c05b010a77322&pid=1-s2.0-S1567539424001889-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A signal-on photoelectrochemical aptasensor based on WO3/CdS heterojunction for the ultrasensitive detection of kanamycin 基于 WO3/CdS 异质结的信号光电化学适配传感器，用于超灵敏检测卡那霉素

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-20 DOI: 10.1016/j.bioelechem.2024.108828

Ying Li , Shiang Wang , Gaiping Li , Chi Zhang , Lina Zou

{"title":"A signal-on photoelectrochemical aptasensor based on WO3/CdS heterojunction for the ultrasensitive detection of kanamycin","authors":"Ying Li , Shiang Wang , Gaiping Li , Chi Zhang , Lina Zou","doi":"10.1016/j.bioelechem.2024.108828","DOIUrl":"10.1016/j.bioelechem.2024.108828","url":null,"abstract":"<div><div>In this study, a signal-on photoelectrochemical (PEC) aptasensor for the ultrasensitive determination of kanamycin (KANA) was constructed using WO<sub>3</sub>/CdS heterojunction as photoactive material. Firstly, WO<sub>3</sub>/CdS heterojunction with excellent photoelectric response was successfully prepared by simple co-precipitation method, resulting in a strong and stable initial photocurrent. In addition, amino modified aptamers were immobilized on the electrode surface by glutaraldehyde as biological recognition components. In the presence of the target KANA, it is specifically recognized and captured by the aptamers. More importantly, KANA can act as a signal amplifier to enhance the photocurrent due to the oxidation of KANA by photogenerated holes. Therefore, a signal-on PEC aptasensor based on WO<sub>3</sub>/CdS heterojunction with high selectivity was obtained for the detection of KANA. Under optimized experimental conditions, the PEC aptasensor demonstrated a wide linear range of 10 pM to 400 nM, with a detection limit of 6.77 pM. Meanwhile, the designed PEC aptasensor had been successfully utilized for the analytical examination of milk, fish, serum, and water samples.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108828"},"PeriodicalIF":4.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001907/pdfft?md5=2a8b0c46c2a381bf4dafb87327270136&pid=1-s2.0-S1567539424001907-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical biosensor based on functional nanomaterials and horseradish peroxidase for the determination of luteolin in peanut shell, honeysuckle and perilla 基于功能纳米材料和辣根过氧化物酶的电化学生物传感器测定花生壳、金银花和紫苏中的木犀草素

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-20 DOI: 10.1016/j.bioelechem.2024.108827

Yu-Jie Chen , Yu-Ying Li , Bao-Lin Xiao , Lin-Lin Ma , Ke-Xin Xu , Sanad Abdalbage Mohammed Abdalsadeg , Tao Hong , Ali Akbar Moosavi-Movahedi , Reza Yousefi , Yan-Na Ning , Jun Hong

{"title":"Electrochemical biosensor based on functional nanomaterials and horseradish peroxidase for the determination of luteolin in peanut shell, honeysuckle and perilla","authors":"Yu-Jie Chen , Yu-Ying Li , Bao-Lin Xiao , Lin-Lin Ma , Ke-Xin Xu , Sanad Abdalbage Mohammed Abdalsadeg , Tao Hong , Ali Akbar Moosavi-Movahedi , Reza Yousefi , Yan-Na Ning , Jun Hong","doi":"10.1016/j.bioelechem.2024.108827","DOIUrl":"10.1016/j.bioelechem.2024.108827","url":null,"abstract":"<div><div>Constructing a biosensor to detect luteolin content accurately is essential, especially considering its specific health benefits at certain concentrations. In this work, the reaction of HRP catalyzed luteolin could be successfully applied in electrocatalytic processes, the oxidation process of electron loss and dehydrogenation occurring on the electrode replaced the hydrogen receptor role of H<sub>2</sub>O<sub>2</sub> in the HRP biocatalytic process. This oxidation reaction had an apparent current response, thus achieving accurate measurement of luteolin. On this biosensor, CTAB was used to disperse MWCNTs, and BSA was used to improve the hydrophobicity of MWCNTs, which was conducive to the subsequent AuNPs fixation of HRP. Three detection methods (LSV, DPV and SWV) for the detection of luteolin were compared and showed that SWV method had a wider linear range (1 × 10<sup>−8</sup>–2 × 10<sup>−5</sup> M) and lower detection limit (8 × 10<sup>−10</sup> M). The determination of luteolin in Traditional Chinese Medicine (TCM) by high performance liquid chromatography (HPLC) and biosensor was almost identical. Therefore, this biosensor could successfully replace HPLC in detecting luteolin in TCM.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108827"},"PeriodicalIF":4.8,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001890/pdfft?md5=faf1ddc86a7e01abbc3f8226967d9fab&pid=1-s2.0-S1567539424001890-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bimodal functionality of highly conductive nanostructured silver film towards improved performance of photosystem I-based graphene photocathode 高导电性纳米结构银膜的双模功能，用于提高基于光系统 I 的石墨烯光电阴极的性能。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-19 DOI: 10.1016/j.bioelechem.2024.108825

Marcin Szalkowski , Małgorzata Kiliszek , Ersan Harputlu , Miriam Izzo , C. Gokhan Unlu , Sebastian Mackowski , Kasim Ocakoglu , Joanna Kargul , Dorota Kowalska

引用次数: 0

PdPtCu mesoporous nanocube-based electrochemical sandwich immunosensor for detection of HIV-p24 基于 PdPtCu 介孔纳米立方体的电化学夹心免疫传感器用于检测 HIV-p24

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-18 DOI: 10.1016/j.bioelechem.2024.108819

Xin Li , Daobin Han , Xinmin Li , Chunjie Zhou , Bo Shen , Honglu Wei , Qian Lou , Changjin Liu , Tingmei Chen

{"title":"PdPtCu mesoporous nanocube-based electrochemical sandwich immunosensor for detection of HIV-p24","authors":"Xin Li , Daobin Han , Xinmin Li , Chunjie Zhou , Bo Shen , Honglu Wei , Qian Lou , Changjin Liu , Tingmei Chen","doi":"10.1016/j.bioelechem.2024.108819","DOIUrl":"10.1016/j.bioelechem.2024.108819","url":null,"abstract":"<div><p>The construction of simple, stable, low-cost and reproducible enzyme-free electrochemical biosensors can effectively avoid the problem of signal attenuation caused by enzyme inactivation. Hererin, we prepared a novel nanoenzymes PdPtCu mesoporous nanocubes (MNCs) to construct a label-free sandwich electrochemical immunosensor for the highly sensitivity detection of HIV-p24. PdPtCu MNCs have excellent peroxidase activity against hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) due to their synergistic ternary composition, large surface area and ability to penetrate mesoporous channels. Moreover, highly conductive and biocompatible gold nanoparticles@graphene oxide (AuNPs@GO) was introduced as a substrate to modify a glassy carbon electrode (GCE). Owing to the excellent electrochemical performance of the PdPtCu MNCs and AuNPs@GO, the developed immunosensors exhibited a good linear response from 0.04 pg/mL to 100 ng/mL with a low detection limit of 20 fg/mL. In addition, the established method exhibited excellent practical performance in human serum. This novel strategy provides a promising platform for ultrasensitive detection of the HIV-p24 in the field of clinical diagnostics.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108819"},"PeriodicalIF":4.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001816/pdfft?md5=837ed1921dd7e6f77374cc56e7d11833&pid=1-s2.0-S1567539424001816-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurement and comparison of dielectric properties of human pancreatic tumours, healthy tissues and porcine tissues ex vivo between 1Hz and 1MHz 在 1Hz 和 1MHz 之间测量和比较人体胰腺肿瘤、健康组织和猪组织的活体介电特性

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-17 DOI: 10.1016/j.bioelechem.2024.108821

Théo Le Berre , Julien Marchalot , Marie Frénéa-Robin , Jérôme Cros , Frédéric Prat , Guilhem Rival

{"title":"Measurement and comparison of dielectric properties of human pancreatic tumours, healthy tissues and porcine tissues ex vivo between 1Hz and 1MHz","authors":"Théo Le Berre , Julien Marchalot , Marie Frénéa-Robin , Jérôme Cros , Frédéric Prat , Guilhem Rival","doi":"10.1016/j.bioelechem.2024.108821","DOIUrl":"10.1016/j.bioelechem.2024.108821","url":null,"abstract":"<div><p>The dielectric properties of pancreatic tissues from human healthy and tumour-bearing tissues have been extracted from impedance measurement on <em>ex vivo</em>, freshly excised samples. They are compared to pig pancreas samples, measured following the same protocol. The purpose is to add data to the scarce literature on the properties of the human pancreas and pancreatic tumours, for treatment planning, tissue identification and numerical simulations. The conductivity measured at 500 kHz for human healthy pancreas is 0.26 S/m, while the conductivity of tumour-bearing tissues is 0.44 S/m. Those values differ significantly from that listed in the IT IS database at 0.57 S/m, suggesting an update might be to consider. However, measures of relative permittivity are in accordance with the database with a value of approximately 2.3x10<sup>3</sup>. <em>Ex vivo</em> porcine model, while being less conductive than human pancreas with 0.16 S/m at the same frequency, is deemed a relevant model when studying pancreatic applications of electromagnetic fields-based treatments, such as radiofrequency ablation.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108821"},"PeriodicalIF":4.8,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S156753942400183X/pdfft?md5=923a6632fbe84dbd4e3e70d111d5b20d&pid=1-s2.0-S156753942400183X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PolyPyrrole based-impedimetric aptasensor for selective determination of beta-HCG from urine sample 基于聚吡咯的选择性测定尿样中 beta-HCG 的阻抗传感器

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-16 DOI: 10.1016/j.bioelechem.2024.108820

Alparslan Deniz , Tunca Karasu , Erdoğan Özgür , Lokman Uzun

{"title":"PolyPyrrole based-impedimetric aptasensor for selective determination of beta-HCG from urine sample","authors":"Alparslan Deniz , Tunca Karasu , Erdoğan Özgür , Lokman Uzun","doi":"10.1016/j.bioelechem.2024.108820","DOIUrl":"10.1016/j.bioelechem.2024.108820","url":null,"abstract":"<div><p>Herein, a conjugated conducting polymer-based impedimetric aptasensor has been developed to detect beta-human chorionic gonadotropin (bHCG), the one of the important biomarkers in gynecology, from synthetic human urine samples. In this context, gold electrodes were, firstly coated with pyrrole and pyrrole-3-carboxylic acid to obtain the poly(pyrrole-pyrrole-3-carboxylic acid) [poly(Py-PyCOOH)] conductive copolymer by cyclic voltammetry (CV). Then, bHCG-specific peptide aptamer was covalently linked onto the surface via applying a well-known carbodiimide-succinimide chemistry. The sensor developed was characterized to confirm modification steps via both electrochemical methods including CV, electrochemical impedance spectroscopy (EIS), and chronoamperometry and physico-chemically via attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), atomic force microscope (AFM), and contact angle measurements (CA). The analytical performance of the sensor was evaluated in the concentration range from 1 μg/mL to 100 μg/mL for successful detection of bHCG even in the presence of interference agents. The results have also revealed that the sensor could be classified as a promising alternative to its benchmark commercial clinical methods due its superior properties such as cost-friendliness, easy-to-prepare, stable, robust, and selectivity / sensitivity.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108820"},"PeriodicalIF":4.8,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001828/pdfft?md5=2d04cf07154cdc6def7b38529b6de3cd&pid=1-s2.0-S1567539424001828-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142241170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical immunoplatform for the determination of multiple salivary biomarkers of oral diseases related to microbiome dysbiosis 用于测定与微生物群失调有关的口腔疾病的多种唾液生物标记物的电化学免疫平台

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-16 DOI: 10.1016/j.bioelechem.2024.108816

Claudia Ramos-López , Lorena Garcia-Rodrigo , Esther Sánchez-Tirado, Lourdes Agüí, Araceli González-Cortés, Paloma Yáñez-Sedeño, José M. Pingarrón

{"title":"Electrochemical immunoplatform for the determination of multiple salivary biomarkers of oral diseases related to microbiome dysbiosis","authors":"Claudia Ramos-López , Lorena Garcia-Rodrigo , Esther Sánchez-Tirado, Lourdes Agüí, Araceli González-Cortés, Paloma Yáñez-Sedeño, José M. Pingarrón","doi":"10.1016/j.bioelechem.2024.108816","DOIUrl":"10.1016/j.bioelechem.2024.108816","url":null,"abstract":"<div><p>Several diseases of the oral cavity are related to compositional and functional shifts in the oral microbiome. The analysis of saliva is an attractive alternative for the diagnosis and prognosis of these diseases. Samples can be obtained by no invasive procedures and processing is relatively simple. However, sensitive and selective analytical methods are needed to make the diagnosis as specific as possible. In this work, four salivary biomarkers of oral diseases: interleukin-6 (IL-6), receptor activator of NF-kB ligand (RANKL), protein arginine deiminase 4 (PAD4) and the corresponding antibody (anti-PAD-4) were selected as targets for their simultaneous determination using an electrochemical immunosensing platform. Sandwich-type amperometric immunoassays were implemented using horseradish peroxidase (HRP)/H<sub>2</sub>O<sub>2</sub>/hydroquinone (HQ) for application to the analysis of saliva of six volunteers. The developed method provides excellent sensitivity, selectivity, and wide linear ranges with LOD values of 0.09 pg mL<sup>−1</sup> (IL-6), 0.10 pg mL<sup>−1</sup> (RANKL); 0.09 ng mL<sup>−1</sup>(PAD4) and 14.5 ng mL<sup>−1</sup> (anti-PAD4) and allows the accurate analysis of saliva without matrix effects, using 25 μL of raw sample. The developed methodology is competitive with commercial ELISA kits available only for a single biomarker determination, while the assay for the four biomarkers can be completed in less than two hours.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108816"},"PeriodicalIF":4.8,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001786/pdfft?md5=ca75c4d9894c9e5bf247eefea462122b&pid=1-s2.0-S1567539424001786-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142239863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Entropy-driven amplification reaction and the CRISPR/Cas12a system form the basis of an electrochemical biosensor for E.coli-specific detection 熵驱动的扩增反应和 CRISPR/Cas12a 系统构成了特异性检测大肠杆菌的电化学生物传感器的基础

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-11 DOI: 10.1016/j.bioelechem.2024.108815

Longjian Huang , Wenzhao Zhang , Mingxuan Liu , Yuanxun Gong , Qianli Tang , Kaihua Wang , Xianjiu Liao , Kai Zhang , Jihua Wei

{"title":"Entropy-driven amplification reaction and the CRISPR/Cas12a system form the basis of an electrochemical biosensor for E.coli-specific detection","authors":"Longjian Huang , Wenzhao Zhang , Mingxuan Liu , Yuanxun Gong , Qianli Tang , Kaihua Wang , Xianjiu Liao , Kai Zhang , Jihua Wei","doi":"10.1016/j.bioelechem.2024.108815","DOIUrl":"10.1016/j.bioelechem.2024.108815","url":null,"abstract":"<div><p>We present an innovative biosensor designed for the precise identification of Escherichia coli (<em>E.coli</em>), a predominant pathogen responsible for gastrointestinal infections. <em>E.coli</em> is prevalent in environments characterized by substandard water quality and can lead to severe diarrhea, especially in hospital settings. The device employs entropy-driven reactions to synthesize copious amounts of double-stranded DNA (dsDNA), which, upon binding with crRNA, triggers the CRISPR/Cas12a system’s cleavage mechanism. This process results in the separation of a ferrocene (Fc)-tagged DNA strand from the electrode, enhancing the electrochemical signal for <em>E.coli</em>’s rapid and accurate detection. Our tests confirm the biosensor’s ability to quantify <em>E.coli</em> across a dynamic range from 100 to 10 million CFU/mL, achieving a detection threshold of just over 5 CFU/mL. The development of this electrochemical biosensor highlights its exceptional selectivity, high sensitivity, and user-friendly interface for <em>E.coli</em> detection. It stands as a significant step forward in pathogen detection technology, promising new directions for identifying various bacterial infections through the CRISPR/Cas mechanism.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108815"},"PeriodicalIF":4.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567539424001774/pdfft?md5=883639504a8697375060e245d3576363&pid=1-s2.0-S1567539424001774-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0