Bioelectrochemistry最新文献_第7页

Iron-based anodes in CW-MFCs: mechanisms and performance enhancement for contaminant removal and sulfamethoxazole elimination cw - mfc中的铁基阳极：去除污染物和磺胺甲恶唑的机制和性能增强

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-11-23 DOI: 10.1016/j.bioelechem.2025.109177

Feng Liu , Panpan Cui , Yun Zhang , Wanyou Qian , Jiancheng Zha , Lei Sun , Yijian Li , Yue Shen , Mi Deng , Jieran Xie

{"title":"Iron-based anodes in CW-MFCs: mechanisms and performance enhancement for contaminant removal and sulfamethoxazole elimination","authors":"Feng Liu , Panpan Cui , Yun Zhang , Wanyou Qian , Jiancheng Zha , Lei Sun , Yijian Li , Yue Shen , Mi Deng , Jieran Xie","doi":"10.1016/j.bioelechem.2025.109177","DOIUrl":"10.1016/j.bioelechem.2025.109177","url":null,"abstract":"<div><div>In recent years, the constructed wetland–microbial fuel cell (CW-MFC) had emerged as a promising technology for antibiotic removal from water, with anode material playing a critical role in system performance. This study investigated the effects of three anode materials—activated carbon (ACCW-MFC), iron-based biochar (FCW-MFC), and pyrite (PCW-MFC)—on the removal of conventional pollutants and sulfamethoxazole (SMX), power generation, and microbial community composition. Results demonstrated that FCW–MFC achieved exceptional removal rates for COD (96.4 ± 0.68 %), TP (100 %), and SMX (100 %). FCW–MFC exhibited superior resilience to shock loads, while PCW–MFC displayed peak voltages of 402 and 399 mV before and after SMX addition, respectively. <em>Pseudomonadota</em> was the dominant bacterial phylum in all three MFCs (ACCW–MFC, FCW–MFC, and PCW–MFC), with relative abundances of 71.34 %, 67.26 %, and 63.35 %, respectively. Notably, FCW–MFC and PCW–MFC supported substantial populations of the denitrifying genus <em>Thauera</em> (27.96 % and 15.58 %, respectively). The study demonstrated that iron amendment in the anode significantly enriches the electroactive microbial community and enhanced the comprehensive performance of CW–MFCs, providing an effective strategy for simultaneous wastewater treatment and energy recovery.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109177"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145616679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating cyclic voltammetry, fluorescence, and docking to elucidate DNA-Schiff-base ligand interactions 整合循环伏安法，荧光，对接阐明dna -希夫碱配体相互作用

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-11-21 DOI: 10.1016/j.bioelechem.2025.109175

Sudabeh Shokrollahi, Ahmad Amiri

{"title":"Integrating cyclic voltammetry, fluorescence, and docking to elucidate DNA-Schiff-base ligand interactions","authors":"Sudabeh Shokrollahi, Ahmad Amiri","doi":"10.1016/j.bioelechem.2025.109175","DOIUrl":"10.1016/j.bioelechem.2025.109175","url":null,"abstract":"<div><div>Schiff-base ligands are versatile coordination compounds with notable biological and electrochemical importance and understanding their interactions with DNA is essential for exploring their potential pharmacological and sensing applications. This study investigates the binding mechanisms between twelve structurally distinct Schiff-base ligands and calf thymus DNA (ct-DNA), emphasizing the influence of electronic and steric factors on binding affinity. Fluorescence spectroscopy, cyclic voltammetry (CV), and molecular docking were combined to evaluate binding constants, quenching behavior, and molecular interactions. Fluorescence titrations revealed moderate-to-strong binding affinities (K<sub>b</sub> = 2.07–9.61 × 10<sup>3</sup> M<sup>−1</sup>) with predominantly static quenching. Ligands containing planar aromatic systems and electron-withdrawing substituents exhibited stronger binding, whereas bulky or methoxy-substituted analogues showed weaker interactions. These observations were further supported by CV studies, showing decreased redox currents accompanied by potential shifts upon formation of DNA-ligand complex. Molecular docking supported the experimental findings, highlighting hydrogen bonding, π-π stacking, and hydrophobic interactions as dominant stabilizing forces. Overall, the integrated spectroscopic, electrochemical, and computational analyses demonstrate a clear structure-activity relationship, establishing that ligand planarity and electronic properties are key determinants of DNA-binding strength and illustrating the potential of cyclic voltammetry as a complementary tool for studying biomolecular interactions.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109175"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145616678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferutinin can form transient pores in lipid membranes in the presence of magnesium or calcium ions 在镁离子或钙离子存在的情况下，阿魏素可以在脂质膜上形成短暂的孔隙。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-12-15 DOI: 10.1016/j.bioelechem.2025.109201

Zaret G. Denieva , Alexander M. Firsov , Ljudmila S. Khailova , Sergey A. Akimov , Elena A. Kotova , Oleg V. Batishchev , Yuri N. Antonenko

{"title":"Ferutinin can form transient pores in lipid membranes in the presence of magnesium or calcium ions","authors":"Zaret G. Denieva , Alexander M. Firsov , Ljudmila S. Khailova , Sergey A. Akimov , Elena A. Kotova , Oleg V. Batishchev , Yuri N. Antonenko","doi":"10.1016/j.bioelechem.2025.109201","DOIUrl":"10.1016/j.bioelechem.2025.109201","url":null,"abstract":"<div><div>The natural phytoestrogen ferutinin acts as a divalent cation-selective ionophore, promoting the opening of Ca<sup>2+</sup>-activated permeability transition pore (PTP) in mitochondria. Here, we found that Mg<sup>2+</sup> cations, known to suppress PTP, markedly augmented the ferutinin-induced permeabilization of rat liver mitochondria. To uncover the permeabilization mechanism, we measured calcein flow from large (LUV) and giant (GUV) unilamellar vesicles. In both cases, the ferutinin-induced calcein efflux was significantly enhanced in the presence of Ca<sup>2+</sup> or Mg<sup>2+</sup>. Calcein influx into GUV stopped within 15-min incubation with ferutinin and Ca<sup>2+</sup>. In electrophysiological experiments, ferutinin caused a smooth increase in the electrical current through planar bilayer lipid membranes (BLM) in the presence of Ca<sup>2+</sup> or Mg<sup>2+</sup>, followed by burst of fluctuations in the current at its high level. In patch-clamp BLM experiments, we detected transient channel activity upon the addition of ferutinin together with Ca<sup>2+</sup>, diminishing over minutes time scale. We propose permeabilization mechanism which involves adsorption of ferutinin on lipid membranes, its dimerization upon binding Mg<sup>2+</sup> or Ca<sup>2+</sup>, leading to mechanical stress induction, and ultimately to membrane pore formation. The mechanical stress is then gradually relieved due to translocation of ferutinin dimers and lipid molecules to the opposite lipid monolayer, resulting in pore closure.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109201"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145779673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Optimized hydrogel environment for a tyrosinase-based sandwich-type phenol biosensor: Application of modeling results to real samples 基于酪氨酸酶的三明治型苯酚生物传感器的优化水凝胶环境：建模结果在实际样品中的应用

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-11-22 DOI: 10.1016/j.bioelechem.2025.109176

Marianela Zoratti , Valeria Pfaffen , Fernando Garay

{"title":"Optimized hydrogel environment for a tyrosinase-based sandwich-type phenol biosensor: Application of modeling results to real samples","authors":"Marianela Zoratti , Valeria Pfaffen , Fernando Garay","doi":"10.1016/j.bioelechem.2025.109176","DOIUrl":"10.1016/j.bioelechem.2025.109176","url":null,"abstract":"<div><div>This work reports the development and optimization of a tyrosinase-based amperometric biosensor for the detection of oxidizable phenols in tea infusions. The enzyme was immobilized within a hydrogel matrix composed of chitosan and mucin, crosslinked with diluted glutaraldehyde. Response Surface Methodology combined with the Desirability Function was employed to optimize the enzymatic matrix, maximizing sensitivity while minimizing response time. The optimal matrix composition corresponds to 50 % chitosan and 50 % mucin crosslinked with 5 % diluted glutaraldehyde. Different enzyme loadings were evaluated to improve the trade-off between sensitivity and linear range. Experimental and theoretical results indicated that high enzyme loadings enhanced sensitivity but restricted the linear range due to oxygen depletion and promoted phenolic polymerization near the active sites, affecting stability. Conversely, lower loadings provided extended linear ranges with only a minor effect on the detection limit. An enzymatic loading of 13 U/sensor was selected as the best compromise between sensitivity, stability, and cost. The optimized biosensor enabled rapid and reproducible quantification of oxidizable phenols in green tea, black tea, and yerba mate infusions, with analysis times below one hour. Results were consistent with literature values and highlighted the necessity of applying a standard addition method to account for matrix effects.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109176"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145616675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroformation of Giant Unilamellar vesicles: Novel electrode design and parameters for enhanced GUVs production 巨型单层囊泡的电形成：新型电极设计和参数用于增强guv的生产。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-11-30 DOI: 10.1016/j.bioelechem.2025.109192

Davide Romanini , Miriam Di Martino , Lucia Sessa , Simona Concilio , Stefano Piotto

{"title":"Electroformation of Giant Unilamellar vesicles: Novel electrode design and parameters for enhanced GUVs production","authors":"Davide Romanini , Miriam Di Martino , Lucia Sessa , Simona Concilio , Stefano Piotto","doi":"10.1016/j.bioelechem.2025.109192","DOIUrl":"10.1016/j.bioelechem.2025.109192","url":null,"abstract":"<div><div>The formation of Giant Unilamellar Vesicles (GUVs) is a critical technology with applications in drug delivery and the study of cellular membranes. This work presents optimized electrode designs and parameters for the electroformation of GUVs. Conventional indium tin oxide (ITO) electrodes are fragile and have limited lifespans. Meanwhile, stainless steel offers mechanical robustness, reusability, and chemical stability due to its chromium oxide layer, particularly in aqueous buffers at near-neutral pH. Here, stainless steel electrodes with different geometries were tested as a cost-effective alternative. The influence of electrode shape, alternating current (AC) frequency, and applied voltage on vesicle yield and size was systematically investigated. Four chamber configurations were evaluated and optimized for electrical resistance. Broad stainless steel mesh 30 electrodes produced the highest vesicle yield, associated with larger surface area and favorable voltage–frequency combinations. Results indicate that electrode shape and electroformation parameters significantly affect GUV characteristics. Stainless steel electrodes can replace ITO electrodes, enabling robust and scalable GUV production. This approach supports applications in biophysics, drug delivery, and biosensor development while reducing material costs and improving operational durability.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109192"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145666537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smart nanostructured electrodes integrated with microbial fuel cells for wastewater-to-energy conversion and on-site detection of antibiotic in real wastewater 集成了微生物燃料电池的智能纳米结构电极，用于废水-能源转换和实际废水中抗生素的现场检测

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-12-23 DOI: 10.1016/j.bioelechem.2025.109211

Tukendra Kumar, Satya Eswari Jujjavarapu

{"title":"Smart nanostructured electrodes integrated with microbial fuel cells for wastewater-to-energy conversion and on-site detection of antibiotic in real wastewater","authors":"Tukendra Kumar, Satya Eswari Jujjavarapu","doi":"10.1016/j.bioelechem.2025.109211","DOIUrl":"10.1016/j.bioelechem.2025.109211","url":null,"abstract":"<div><div>Detection of ciprofloxacin (CIP) is crucial due to its persistence and frequent occurrence in water bodies. An algal microbial fuel cell (MFC) integrated with an electrospun carbon nanotube–polyaniline–zinc oxide (CNT–PANI–ZnO) nanofiber electrode was developed for simultaneous renewable energy generation and CIP detection. The electrode enhanced electron transfer efficiency, enabling sensitive monitoring of voltage changes during CIP analysis. The electrode properties were characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), ultraviolet–visible (UV–Vis) spectroscopy, zeta potential, and contact angle analyses. Bio-electrochemical performance was assessed using linear sweep voltammetry (LSV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The algal MFC achieved a maximum power density of 27.57 ± 0.95 mW m<sup>−3</sup> at a current density of 46.73 ± 0.57 mA m<sup>−2</sup> with an internal resistance of 22 Ω. CIP was detected with a minimum limit of 0.5 μg L<sup>−1</sup> and a sensitivity of 0.1568 mV (μg L<sup>−1</sup>)<sup>−1</sup> under stable voltage conditions. The system exhibited reusability over three operational cycles. These findings demonstrate the potential of CNT–PANI–ZnO-based algal MFCs as eco-friendly platforms for sustainable energy conversion and real-time contaminant sensing.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109211"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances and perspectives on N2 fixation by microbial bioelectrochemical systems 微生物电化学系统固氮研究进展与展望

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-03-01 Epub Date: 2025-09-24 DOI: 10.1016/j.bioelechem.2025.109123

Axel Rous , James A. Behan , Elie Desmond-Le Quéméner , Nicolas Bernet , Eric Trably , Frédéric Gloaguen , Frédéric Barrière

{"title":"Recent advances and perspectives on N2 fixation by microbial bioelectrochemical systems","authors":"Axel Rous , James A. Behan , Elie Desmond-Le Quéméner , Nicolas Bernet , Eric Trably , Frédéric Gloaguen , Frédéric Barrière","doi":"10.1016/j.bioelechem.2025.109123","DOIUrl":"10.1016/j.bioelechem.2025.109123","url":null,"abstract":"<div><div>This review covers recent advances in the fixing of dinitrogen in microbial bioelectrochemical systems (BES) where bacteria release or accept electron to/from electrodes for their respiratory metabolism, either directly or indirectly. We discuss how BES may be interesting platforms for producing ammonium or biomass from N<sub>2</sub> fixation. The potential for N<sub>2</sub>-fixation in BES is first discussed with a focus on possible metabolism and different mechanism that may lead to an increase of fixed dinitrogen. We then review recent examples where dinitrogen is fixed at the cathodes of BES, either by pure cultures of hydrogenotrophic and/or diazotrophic bacteria using cathodic H<sub>2</sub> or reduced redox mediators as the electron, or by mixed enriched consortia. A section is then devoted to the special case of nitrogen fixation at anodic microbial electrode where organic matter oxidation also occurs. Finally, a comparison of the reported current performance of nitrogen fixation in BES with other biotic (anerobic digestion) or abiotic (Haber-Bosch process, electrochemical N<sub>2</sub> reduction) is provided together with a perspective on possible optimization and application of this emerging microbial electrochemical and technological process.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109123"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laccase-mimicking nAuMnOx integrated into a molecularly imprinted electrochemical sensor for selective homovanillic acid detection in human urine 模拟漆酶的nAuMnOx集成到分子印迹电化学传感器中，用于选择性检测人类尿液中的同型香草酸。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-03-01 Epub Date: 2025-10-29 DOI: 10.1016/j.bioelechem.2025.109155

Olha Demkiv , Nataliya Stasyuk , Marcin Holdynski , Wojciech Nogala , Mykhailo Gonchar

{"title":"Laccase-mimicking nAuMnOx integrated into a molecularly imprinted electrochemical sensor for selective homovanillic acid detection in human urine","authors":"Olha Demkiv , Nataliya Stasyuk , Marcin Holdynski , Wojciech Nogala , Mykhailo Gonchar","doi":"10.1016/j.bioelechem.2025.109155","DOIUrl":"10.1016/j.bioelechem.2025.109155","url":null,"abstract":"<div><div>A novel bioelectrochemical sensor has been developed for the selective detection of homovanillic acid (HVA), an important urinary biomarker of neuroblastoma. The sensor integrates green-synthesized bimetallic gold–manganese nanoparticles (nAuMnO<sub>x</sub> NPs) with laccase-like catalytic activity into a molecularly imprinted polymer (MIP) layer on a graphite electrode (MIP/nAuMnO<sub>x</sub>/GE). The nanozyme was synthesized using gallic acid as a biocompatible reducing and stabilizing agent. The MIP was formed from (3-aminopropyl)triethoxysilane as a functional monomer providing amine groups for HVA binding, tetramethyl orthosilicate as a cross-linker forming a stable silica network, and β-cyclodextrin as a supramolecular host enabling selective inclusion of the aromatic part of HVA. This composition imparted excellent molecular recognition and electrocatalytic activity. The MIP/nAuMnO<sub>x</sub>/GE sensor showed a high response at −200 mV with a limit of detection 0.12 μM and a linear range of 1–30 μM. Sensitivity reached up to 1.39 × 10<sup>4</sup> A·M<sup>−1</sup>·m<sup>−2</sup>, representing a 14-fold enhancement over natural laccase-based sensors. The sensor demonstrated outstanding selectivity toward HVA against dopamine, 5-hydroxyindoleacetic acid, vanillylmandelic acid, uric, and ascorbic acids, and enabled direct HVA quantification in untreated human urine with recovery rates of 97.5–102 %. The synergistic integration of nAuMnO<sub>x</sub> nanozyme catalysis and MIP recognition establishes a robust platform for noninvasive neuroblastoma diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109155"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145457087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of microbiologically influenced corrosion in 90/10 CuNi alloy by a novel antibacterial peptide 新型抗菌肽对90/10 CuNi合金微生物腐蚀的抑制作用

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-03-01 Epub Date: 2025-09-16 DOI: 10.1016/j.bioelechem.2025.109114

Nianting Xue, Hao Sun, Shihang Lu, Yizhen Yu, Wenyan Ma, Guangzhou Liu, Wenwen Dou

{"title":"Inhibition of microbiologically influenced corrosion in 90/10 CuNi alloy by a novel antibacterial peptide","authors":"Nianting Xue, Hao Sun, Shihang Lu, Yizhen Yu, Wenyan Ma, Guangzhou Liu, Wenwen Dou","doi":"10.1016/j.bioelechem.2025.109114","DOIUrl":"10.1016/j.bioelechem.2025.109114","url":null,"abstract":"<div><div>Microbiologically influenced corrosion (MIC) poses a serious threat to the structural integrity of Cu<img>Ni alloys under marine service conditions. This study proposed a novel antibacterial peptide (Tcs) combined with Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) to inhibit MIC in 90/10 Cu<img>Ni alloy induced by <em>Desulfovibrio ferrophilus</em> IS5. Bacterial counts, environmental parameters analysis, surface morphological analyses, weight loss measurements, and electrochemical tests were conducted, through which the antibacterial and corrosion inhibition effects were demonstrated. The results showed that 180 ppb Peptide Tcs (180 Tcs) alone exerted almost no inhibitory effect on <em>D. ferrophilus</em> IS5, whereas a significant concentration-dependent enhancement was observed when Peptide Tcs was combined with 20 ppm ADBAC (20 ADBAC). Specifically, the 20 ppm ADBAC +180 ppb Peptide Tcs (20 ADBAC-180 Tcs) combination further reduced sessile bacterial counts by one order of magnitude compared to 20 ADBAC alone, while the weight loss rate and maximum pitting depth decreased by approximately 86 % and 61 %, respectively. These findings provide new insights into optimizing traditional biocide systems with small antimicrobial peptides and developing green, efficient strategies for controlling marine MIC.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109114"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of phenylboronic acid modified graphene oxide-silica biosensor for label-free electrochemical detection of circulating tumor cells 用于循环肿瘤细胞无标记电化学检测的苯基硼酸修饰氧化石墨烯-二氧化硅生物传感器的制备

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-03-01 Epub Date: 2025-09-01 DOI: 10.1016/j.bioelechem.2025.109098

Sabeen Iqbal , Fahmida Jabeen , Nabila Bashir , Syed Tayyab Raza Naqvi , Saadat Majeed , Azhar Rasul , Muhammad Najam-ul-Haq

{"title":"Fabrication of phenylboronic acid modified graphene oxide-silica biosensor for label-free electrochemical detection of circulating tumor cells","authors":"Sabeen Iqbal , Fahmida Jabeen , Nabila Bashir , Syed Tayyab Raza Naqvi , Saadat Majeed , Azhar Rasul , Muhammad Najam-ul-Haq","doi":"10.1016/j.bioelechem.2025.109098","DOIUrl":"10.1016/j.bioelechem.2025.109098","url":null,"abstract":"<div><div>Early and accurate detection of circulating tumor cells (CTCs) is vital for cancer diagnosis and personalized treatment. Despite their clinical significance, the identification of CTCs remains challenging because of the biological complexity and lower concentration. Therefore, a cost-effective, and label-free electrochemical biosensor based on phenyl boronic acid functionalized graphene oxide-silica (PBA@GO-SiO<sub>2</sub>) is developed to selectively recognize sialic acid-rich glycoproteins on HepG2 cells. The electrochemical properties of developed biosensor confirm a concentration-dependent response upon interaction with HepG2 cells. The biosensor demonstrates a broad linear range of 50–10<sup>5</sup> cells/mL with LOD of 26 cells/mL. Electrochemical surface area (ECSA) and optimization studies verify the stable sensor performance across different scan rates and pH conditions. It also exhibits selectivity, reproducibility, and reusability while retaining 85 % original signal after multiple regeneration cycles. Overall, PBA@GO-SiO<sub>2</sub> based biosensor is a scalable platform requiring small blood sample, eliminating the need for antibodies, ensuring long shelf life because of durable material, and carry translational potential at clinical level.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109098"},"PeriodicalIF":4.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0