Bioelectrochemistry最新文献_第6页

A bio-inspired strategy using hydrophobic bacterial EPS for the design of ZIF-8-based anti-corrosion coatings with secondary sealing function 利用疏水性细菌EPS设计具有二次密封功能的zif -8基防腐涂料的仿生策略

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-12-15 DOI: 10.1016/j.bioelechem.2025.109202

Jiajie Wu , Huanhuan Wei , Tingting Zhu , Zhen Li , Guang Li , Yuanyuan Guo , Zhuangzhuang Bi , Bo Wu , Qing Qu

{"title":"A bio-inspired strategy using hydrophobic bacterial EPS for the design of ZIF-8-based anti-corrosion coatings with secondary sealing function","authors":"Jiajie Wu , Huanhuan Wei , Tingting Zhu , Zhen Li , Guang Li , Yuanyuan Guo , Zhuangzhuang Bi , Bo Wu , Qing Qu","doi":"10.1016/j.bioelechem.2025.109202","DOIUrl":"10.1016/j.bioelechem.2025.109202","url":null,"abstract":"<div><div>Developing sustainable corrosion-resistant coatings remains a key challenge for protecting metals in marine environments. Here, we report a bio-inspired hybrid coating that integrates naturally hydrophobic soluble extracellular polymeric substances (s-EPS) derived from marine <em>Bacillus subtilis</em> with the metal-organic framework ZIF-8. The s-EPS@ZIF-8 composite is formed through electrostatic attraction and coordination between Zn<sup>2+</sup> nodes of ZIF-8 and the functional groups (-COOH, -OH, -NH<sub>2</sub>) of s-EPS, enabling a uniform and compact organic-inorganic network. The hydrophobicity of s-EPS originates from amphiphilic protein components such as BslA and the molecular rearrangement that exposes hydrophobic domains during film formation. This synergy produces a dense superhydrophobic layer on X70 carbon steel with a water contact angle of 131.7<sup>°</sup>. Structural and chemical analyses confirm coherent incorporation of s-EPS within the ZIF-8 framework without compromising its crystallinity. Electrochemical tests reveal superior corrosion resistance, with inhibition efficiencies of 94.54 % after 2 days and 94.84 % after 30 days in artificial seawater. The coating functions via a cooperative formation-stabilisation-reinforcement mechanism, providing durable, self-reinforcing barrier protection. This strategy demonstrates the potential of combining naturally hydrophobic marine biopolymers with MOF frameworks for sustainable, high-performance anticorrosion coatings in marine environments.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109202"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145779689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wearable (bio)sensors for wound healing monitoring: recent achievements, challenges, and future directions 用于伤口愈合监测的可穿戴（生物）传感器：最近的成就、挑战和未来方向

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-12-18 DOI: 10.1016/j.bioelechem.2025.109204

J. Czopinska, I. Zaras, M. Jarczewska

{"title":"Wearable (bio)sensors for wound healing monitoring: recent achievements, challenges, and future directions","authors":"J. Czopinska, I. Zaras, M. Jarczewska","doi":"10.1016/j.bioelechem.2025.109204","DOIUrl":"10.1016/j.bioelechem.2025.109204","url":null,"abstract":"<div><div>Wound treatment costs are estimated to reach over 20 billion USD in the case of chronic wounds. Hence, the methods of monitoring the stage of healing are of high importance. It should be emphasized that traditionally applied methods, including visual examination as well as bacterial cell cultivation, are not precise and might be lengthy. Therefore, there is a great need for elaboration of novel approaches in controlling the healing process as well as enhancing it through novel drug delivery systems that can be realized using wearable sensors. Such an approach contributes to the minimization of patient discomfort and enables the live determination of the actual stage of wound healing. Herein, we present a critical review of current examples of such devices (presented from 2021 to 2025), as well as the characterization of basics such as the wound healing process and materials applied for sensors' fabrication. In the final part, we depict the challenges, including signal drift or nonspecific adsorption on the sensor surface, and future perspectives of the application of such noninvasive sensors in clinical diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109204"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145786523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interfacing nitrogen biochemistry with electrochemical output in Saccharomyces cerevisiae microbial fuel cells 酿酒酵母微生物燃料电池中氮生化与电化学输出的界面研究

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-11-23 DOI: 10.1016/j.bioelechem.2025.109178

Marcelinus Christwardana , Muhammad Fahrul Riza , Purbowatiningrum Ria Sarjono

{"title":"Interfacing nitrogen biochemistry with electrochemical output in Saccharomyces cerevisiae microbial fuel cells","authors":"Marcelinus Christwardana , Muhammad Fahrul Riza , Purbowatiningrum Ria Sarjono","doi":"10.1016/j.bioelechem.2025.109178","DOIUrl":"10.1016/j.bioelechem.2025.109178","url":null,"abstract":"<div><div>Microbial fuel cells (MFCs) utilize microbial metabolism to convert organic substrates into electrical energy. <em>Saccharomyces cerevisiae</em> presents a promising eukaryotic biocatalyst due to its fermentative capacity and non-pathogenic nature, yet its electron transfer efficiency remains a major bottleneck. This study evaluates the influence of nitrogen source variation, peptone, tryptone, and bovine serum albumin (BSA) at concentrations of 1, 2.5, and 5 mg.mL<sup>−1</sup> on the electrochemical performance of <em>Saccharomyces cerevisiae</em>-based MFCs. Half-cell analyses, including cyclic voltammetry and rate-determining step (RDS) assessments, revealed diffusion-controlled electron transfer via cytochromes. The highest electron transfer rate constant (<em>K<sub>s</sub></em>) was obtained with peptone 5 mg.mL<sup>−1</sup> (1.61 ± 0.285 s<sup>−1</sup>), followed by tryptone 1 mg.mL<sup>−1</sup> (1.53 ± 0.332 s<sup>−1</sup>) and BSA 1 mg.mL<sup>−1</sup> (0.95 ± 0.055 s<sup>−1</sup>). Full-cell experiments showed maximum voltage outputs of 0.132 V (peptone 5 mg.mL<sup>−1</sup>), 0.117 V (tryptone 1 mg.mL<sup>−1</sup>), and 0.039 V (BSA 1 mg.mL<sup>−1</sup>), and corresponding peak power densities of 46.6, 44.0, and 7.1 mW m<sup>−2</sup>. SEM confirmed enhanced biofilm formation with increased nitrogen concentration, supporting stronger electrochemical activity. These results highlight nitrogen source optimization as a strategic approach to enhance microbial electron transfer and energy yield in yeast-based MFC systems.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109178"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145616676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrochemical biosensing platform based on α‑iron trioxide/ferriferrous oxide magnetic nanocomposites for early diagnosis of hepatocellular carcinoma 基于α -三氧化铁/氧化亚铁磁性纳米复合材料的肝细胞癌早期诊断电化学生物传感平台。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-12-15 DOI: 10.1016/j.bioelechem.2025.109203

Haoda Zhang , Hezhong Ouyang , Zishu Zhou , Tiebin Wan , Yongjin Li , Ruijiang Liu

{"title":"An electrochemical biosensing platform based on α‑iron trioxide/ferriferrous oxide magnetic nanocomposites for early diagnosis of hepatocellular carcinoma","authors":"Haoda Zhang , Hezhong Ouyang , Zishu Zhou , Tiebin Wan , Yongjin Li , Ruijiang Liu","doi":"10.1016/j.bioelechem.2025.109203","DOIUrl":"10.1016/j.bioelechem.2025.109203","url":null,"abstract":"<div><div>The detection of biologically active proteins in complex biological matrices such as serum remains challenging. To enhance the sensitivity and accuracy of detecting the hepatocellular carcinoma biomarker stress-induced phosphoprotein 1 (STIP1), we proposed a label-free “Turn-off” electrochemical aptamer-based biosensing platform without requiring amplification steps. The biosensing platform integrated magnetic α-Fe<sub>2</sub>O<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub> (α‑iron trioxide/ferriferrous oxide, αITFO) nanocomposites synthesized via urea hydrolysis followed by calcination-reduction processes and further functionalized with Au nanoparticles (NPs), achieving significantly enhanced electrochemical activity. The moderate magnetism of the αITFO MNCs enabled efficient magnetic separation, while their compatibility with the magnetic glassy carbon electrode (MGCE) allowed rapid sensor fabrication and highly sensitive detection at a low cost. Another notable characteristic of the STIP1 biosensing platform lay in its exceptional detection capabilities, exhibiting excellent sensitivity and a broad detection range of 10 pg mL<sup>−1</sup> to 10 μg mL<sup>−1</sup>, with a remarkably low detection limit (LOD) of 3.29 pg mL<sup>−1</sup>. Furthermore, the STIP1 biosensing platform was successfully implemented for analyzing clinical spiked serum samples, demonstrating recovery rates ranging from 102.2 % to 114.2 %. This validated its accuracy and effectiveness for applications in early disease diagnosis and prognostic evaluation.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109203"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145773062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the nature of the electrochemical response in mediated bioanodes with yeast cells 酵母细胞介导生物阳极的电化学响应性质研究。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-12-13 DOI: 10.1016/j.bioelechem.2025.109197

Ekaterina V. Zolotukhina , Konstantin V. Gor'kov , Maria V. Dmitrieva , Maria G. Levchenko , Yuliya E. Silina

{"title":"On the nature of the electrochemical response in mediated bioanodes with yeast cells","authors":"Ekaterina V. Zolotukhina , Konstantin V. Gor'kov , Maria V. Dmitrieva , Maria G. Levchenko , Yuliya E. Silina","doi":"10.1016/j.bioelechem.2025.109197","DOIUrl":"10.1016/j.bioelechem.2025.109197","url":null,"abstract":"<div><div>Herein, the electrochemical responses of <em>Saccharomyces cerevisiae</em> cultivated in YPD medium were tested in amperometric mode using microanalytical screen-printed electrodes modified with potassium ferricyanide and 1,10-phenanthroline-5,6-dione as mediators. The electrochemical signals obtained correlated well with the yeast growth curve.</div><div>Electrochemical analysis of the cell-free supernatants (CFSs), obtained after removing <em>S. cerevisiae</em> cells, indicated that the mediator response, whether using potassium ferricyanide or phenanthroline-5,6-dione, depends more on the composition of the CFSs than on the intrinsic electroactivity of the cells.</div><div>FT-IR and GC–MS investigations confirmed the formation of several electroactive compounds in the CFSs, such as hydroquinones and organohydrazines. Model electrochemical tests using ferricyanide as redox mediator indicated that quinones and organohydrazines are the most electroactive components formed during microbial activity.</div><div>These results suggest that the electrochemical response in mediated bioanodes containing living cells is primarily driven by interactions between the mediator and electroactive metabolic products, rather than by direct interactions between the mediator and the cells.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109197"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145779664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phage-based dual-mode sensor using ECL and EIS for sensitive detection of Pseudomonas Aeruginosa 基于噬菌体的ECL和EIS双模传感器对铜绿假单胞菌的灵敏检测

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-12-31 DOI: 10.1016/j.bioelechem.2025.109212

Maryam Allahyari , Emre Dokuzparmak , Arzum Erdem , Alper AKKAYA , Bahattin Tanyolac

{"title":"Phage-based dual-mode sensor using ECL and EIS for sensitive detection of Pseudomonas Aeruginosa","authors":"Maryam Allahyari , Emre Dokuzparmak , Arzum Erdem , Alper AKKAYA , Bahattin Tanyolac","doi":"10.1016/j.bioelechem.2025.109212","DOIUrl":"10.1016/j.bioelechem.2025.109212","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> is a major pathogen responsible for severe infections, particularly in immunocompromised patients and those with chronic conditions. Its increasing resistance to antibiotics underscores the urgent need for effective detection systems. This study introduces a novel biosensor based on bacteriophage (M-PAP1) in combination with MWCNT-COOH modified SPE and its application for the sensitive identification of <em>Pseudomonas aeruginosa</em>. The phage provides high specificity and strong target binding, while the combined ECL and EIS modes offer complementary signal outputs, enhancing analytical reliability and reducing false responses. The ECL sensor achieved LoD of 0.755 CFU ml<sup>−1</sup>and demonstrated a broad linear working range of 2.28 to 10<sup>10</sup> CFU ml<sup>−1</sup> (R<sup>2</sup>: 0.9981). In artificial urine samples, the sensor demonstrated a recovery rate of 92% to 97%, indicating its effectiveness in real biological matrices. The dual functionality for both ECL and EIS measurements highlights the system's versatility and potential for real-time clinical applications. The system also exhibited exceptional selectivity and minimal interference from non-specific bacteria such as <em>Escherichia coli</em> and <em>Pseudomonas putida</em> and <em>Pseudomonas fluorescens.</em> Overall, the dual-mode strategy significantly strengthens diagnostic accuracy by enabling cross-validated detection signals, offering a robust platform for rapid monitoring of <em>P. aeruginosa</em> in the context of rising antimicrobial resistance.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109212"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145880310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Minimal bamboo biochar dosing as sediment additive in sediment microbial fuel cells for bioelectricity production and benthic nutrient removal 竹炭作为沉积物微生物燃料电池中用于生物发电和底栖生物营养物去除的沉积物添加剂的最小剂量

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-11-29 DOI: 10.1016/j.bioelechem.2025.109182

Nurfarhana Nabila Mohd Noor, Kyunghoi Kim

{"title":"Minimal bamboo biochar dosing as sediment additive in sediment microbial fuel cells for bioelectricity production and benthic nutrient removal","authors":"Nurfarhana Nabila Mohd Noor, Kyunghoi Kim","doi":"10.1016/j.bioelechem.2025.109182","DOIUrl":"10.1016/j.bioelechem.2025.109182","url":null,"abstract":"<div><div>Sediment resistivity limits the performance of sediment microbial fuel cells (SMFCs) by hindering mass electron transfer in the anodic region. This microcosm study evaluates the effect of bamboo biochar as a conductive additive at different dosages: SMFC-0 (0%), SMFC-0.1 (0.02%), SMFC-1 (0.2%), and SMFC-10 (2%). The study examines the current density, polarization behavior, redox activity, elemental composition, textural properties, anodic biofilm morphology and nutrient removal. The results show that 2% biochar (SMFC-10) increase sediment conductivity by 1.2-fold and reduces ohmic loss for mass electron transfer, achieving the highest power density (26.01 mW/m<sup>2</sup>) and current output (171 mA/m<sup>2</sup>). Field emission scanning electron microscopy (FESEM) analysis reveals dense anodic biofilm formation in SMFC-10, supporting higher bioelectricity generation. SMFC-10 improves pollutant removal and mitigates pollutant release into the overlying water, reducing ammonia‑nitrogen (NH<sub>3</sub>−N) from 5 to 1 mg/L and chemical oxygen demand (COD) from 163 to 33 mg/L. High specific BET surface area (430.15 m<sup>2</sup>/g), small pore size (1.62 nm) and high carbon content (79.46%) of biochar contribute to improve performance and long-term stability (165 days) without nutrient replenishment. These findings demonstrate that bamboo biochar is a promising sediment additive to enhance SMFC power generation and water quality.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109182"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145681734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical sensor for direct HIV-1 DNA detection using ruthenium assembled on a pyrrolidinyl peptide nucleic acid-DNA hybrid 将钌组装在吡咯烷基肽核酸-DNA杂交体上，用于直接检测HIV-1 DNA的电化学传感器。

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-11-22 DOI: 10.1016/j.bioelechem.2025.109172

Khanut Chittuam , Nantanat Lersanansit , Pisit Tangkijvanich , Natthaya Chuaypen , Anchalee Avihingsanon , Tirayut Vilaivan , Mohini Sain , Nattaya Ngamrojanavanich , Sakda Jampasa , Orawon Chailapakul

{"title":"Electrochemical sensor for direct HIV-1 DNA detection using ruthenium assembled on a pyrrolidinyl peptide nucleic acid-DNA hybrid","authors":"Khanut Chittuam , Nantanat Lersanansit , Pisit Tangkijvanich , Natthaya Chuaypen , Anchalee Avihingsanon , Tirayut Vilaivan , Mohini Sain , Nattaya Ngamrojanavanich , Sakda Jampasa , Orawon Chailapakul","doi":"10.1016/j.bioelechem.2025.109172","DOIUrl":"10.1016/j.bioelechem.2025.109172","url":null,"abstract":"<div><div>This study presents an electrochemical DNA biosensor designed for the direct, label-free, and sensitive detection of human immunodeficiency virus type 1 (HIV-1) DNA. The biosensor utilized pyrrolidinyl peptide nucleic acid (acpcPNA) probes immobilized on chitosan/glutaraldehyde-modified screen-printed carbon electrodes to facilitate the duplex invasion process with the target DNA. In the presence of the target DNA backbone, the formed duplex invasion triggered the electrostatic accumulation of a cationic hexaammineruthenium(III) redox indicator, resulting in an increased current response monitored by square wave voltammetry. The biosensor detected synthetic HIV-1 DNA within a linear range of 5–75 nM (limit of detection (LOD): 1.34 nM; limit of quantification (LOQ): 4.44 nM) and clinical HIV-1 cDNA within 10<sup>3</sup>–10<sup>5</sup> copies/mL (LOD: 2.25 × 10<sup>2</sup> copies/mL; LOQ: 7.50 × 10<sup>2</sup> copies/mL), with correlation coefficients of 0.9988 and 0.9905, respectively. Importantly, the proposed platform eliminated the need for thermal denaturation and amplification while demonstrating strong selectivity against mismatched and non-target sequences, as confirmed by RT-PCR. In addition, this flexible platform enabled on-site analysis by integrating successfully with Bluetooth potentiostats and near-field communication reader chips. Given its advantages, this biosensor could serve as a promising prototype and a valuable tool for resource-limited settings requiring the detection of clinically relevant nucleic acid markers.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109172"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145601621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electropriming-mediated modulation of seed germination: Effects on morphology, water uptake, and oxidative status 电激发介导的种子萌发调控：对形态、水分吸收和氧化状态的影响

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-12-09 DOI: 10.1016/j.bioelechem.2025.109195

Gema Cárdenas-Flores , Erika Bustos-Bustos , Efigenia Montalvo-González , Marcelo Victorio-De los Santos , Victor M. Zamora-Gasga , Martina A. Chacón-López , Ulises M. López-García

{"title":"Electropriming-mediated modulation of seed germination: Effects on morphology, water uptake, and oxidative status","authors":"Gema Cárdenas-Flores , Erika Bustos-Bustos , Efigenia Montalvo-González , Marcelo Victorio-De los Santos , Victor M. Zamora-Gasga , Martina A. Chacón-López , Ulises M. López-García","doi":"10.1016/j.bioelechem.2025.109195","DOIUrl":"10.1016/j.bioelechem.2025.109195","url":null,"abstract":"<div><div>Electropriming, the exposure of seeds to direct current electric fields (DCEF) prior to germination, is emerging as a sustainable physical priming strategy to enhance seed vigor and crop performance. In this study, we demonstrated that electropriming markedly accelerated tomato (<em>Solanum lycopersicum</em>) seed germination and induced early physiological adjustments. Treated seeds germinated faster, absorbed water more efficiently, and showed distinct morphological changes compared with non-primed controls. Electropriming also modulated membrane integrity, as evidenced by increased electrolyte leakage, and influenced redox metabolism, indicated by enhanced production of reactive species and activation of antioxidant enzymes. Together, these responses suggest that direct-current electropriming promotes a controlled oxidative burst that primes seeds for faster and more robust germination. Our findings highlight electropriming as a promising chemical-free approach to improve seed vigor, offering new opportunities for sustainable agriculture.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109195"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 2D/3D nanoplatform for electrochemical diagnosis of miRNA-122 in breast cancer cell lines 乳腺癌细胞系miRNA-122电化学诊断的二维/三维纳米平台

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-12-22 DOI: 10.1016/j.bioelechem.2025.109209

Ali Esmaeilian , Nastaran Arab , Fatemeh Yazdian , Morteza Hosseini , Seyed Mohammad Reza Mortazavi , Mohammad Reza Ganjali

{"title":"A 2D/3D nanoplatform for electrochemical diagnosis of miRNA-122 in breast cancer cell lines","authors":"Ali Esmaeilian , Nastaran Arab , Fatemeh Yazdian , Morteza Hosseini , Seyed Mohammad Reza Mortazavi , Mohammad Reza Ganjali","doi":"10.1016/j.bioelechem.2025.109209","DOIUrl":"10.1016/j.bioelechem.2025.109209","url":null,"abstract":"<div><div>A novel, label-free electrochemical biosensor was precise engineered for the ultrasensitive and selective detection of miRNA-122, a pivotal biomarker in breast cancer diagnostics. This innovative sensing platform harnesses the exceptional conductivity of Ti<sub>3</sub>C<sub>2</sub> MXene nanosheets, synergistically integrated with Au-decorated NH<sub>2</sub>-UiO-66 metal-organic frameworks, yielding a structurally stable and electrochemically active hybrid interface. A thiolated DNA capture probe was immobilized onto the gold-modified MOF, enabling precise hybridization with the target miRNA-122. Methylene blue functioned as a robust redox indicator, exhibiting distinct electrochemical shifts upon duplex formation through π–π stacking and electrostatic interactions. The biosensor demonstrated outstanding analytical performance with dual linear response ranges (1 pM–1 nM and 1 nM–750.0 nM) and an impressively low limit of detection of 503 fM. High sequence specificity was achieved, successfully differentiating miRNA-122 from non-complementary and closely related sequences. Validation with lysates from three distinct breast cancer cell lines (MCF-7, MDA-MB-231, and SKBR3) confirmed its efficacy in complex biological matrices. Altogether, this biosensor platform offers a powerful and reliable tool for early breast cancer detection and holds substantial potential for clinical applications in miRNA-based diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"169 ","pages":"Article 109209"},"PeriodicalIF":4.5,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145836794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0