An electrochemical biosensor for detecting pathogenic bacteria based on hybrid nanocomposites

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-07-26 DOI:10.1016/j.bioelechem.2025.109057

Jiaqi Wei , Chunye Wang , TianYu Zhang , Zhuo Hao , Guijuan Qu , Jiayu Wan

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引用次数: 0

Abstract

Prevention and control of foodborne pathogens are essential for public health safety. This study integrates organic-inorganic hybrid nanocomposites, lateral flow immunochromatography, and screen-printed electrodes to develop an innovative electrochemical biosensor for detecting foodborne pathogens. Two types of organic-inorganic hybrid nanocomposites incorporated onto a conjugate pad or nitrocellulose membrane support were used. In the presence of foodborne pathogens, a sandwich immune complex structure forms on the detection line. Ferrocene within the sandwich structure is detected using differential pulse voltammetry. The biosensor specifically detects foodborne pathogens with a minimum detection limit of 25 CFU·mL⁻¹ and a detection linear range of 10² to 10⁸ CFU·mL⁻¹, demonstrating excellent specificity and sensitivity. This biosensor can be integrated with smartphones, enhancing the intelligence and miniaturization of biosensors.

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一种基于杂化纳米复合材料的病原菌检测电化学生物传感器

预防和控制食源性病原体对公共卫生安全至关重要。本研究将有机-无机杂化纳米复合材料、横向流动免疫色谱和丝网印刷电极相结合，开发了一种用于检测食源性病原体的创新电化学生物传感器。采用两种类型的有机-无机杂化纳米复合材料结合到共轭垫或硝化纤维素膜支撑。在食源性病原体存在的情况下，在检测线上形成三明治免疫复合物结构。采用差分脉冲伏安法检测夹芯结构中的二茂铁。该生物传感器对食源性致病菌的特异性检测最低检出限为25 CFU·mL - 1，检测线性范围为102 ~ 108 CFU·mL - 1，具有良好的特异性和灵敏度。这种生物传感器可以与智能手机集成，提高生物传感器的智能化和小型化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.