Bioelectrochemistry最新文献_第5页

Label-free determination of glypican-3 using PtPd@H-rGO nanocomposites decorated light-addressable potentiometric sensor 利用PtPd@H-rGO纳米复合材料修饰光寻址电位传感器无标签测定glypican-3

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-27 DOI: 10.1016/j.bioelechem.2024.108855

Guiyin Li , Wenzhan Li , Yu Zhou , Xiaohong Tan , Qing Huang , Jintao Liang , Zhide Zhou

{"title":"Label-free determination of glypican-3 using PtPd@H-rGO nanocomposites decorated light-addressable potentiometric sensor","authors":"Guiyin Li , Wenzhan Li , Yu Zhou , Xiaohong Tan , Qing Huang , Jintao Liang , Zhide Zhou","doi":"10.1016/j.bioelechem.2024.108855","DOIUrl":"10.1016/j.bioelechem.2024.108855","url":null,"abstract":"<div><div>Glypican-3 (GPC3) is exclusively overexpressed in most Hepatocellular carcinoma (HCC) tissue but not in normal liver tissue, making it a promising biomarker for the precise detection of HCC. In this paper, a label-free light-addressable potentiometric sensor (LAPS) decorated by platinumpalladium-hemin-reduced graphene oxide nanocomposites (PtPd@H-rGO NCs) was constructed for determination of GPC3. The GPC3 aptamer (GPC3<sub>Apt</sub>) and PtPd@H-rGO NCs were modified on the surface of silicon-based LAPS chip to build sensitive unit of LAPS system. A readout photocurrent elicited from a modulated light source, registers the localized surface potential change. When a bias voltage is provided to the LAPS system, the GPC3-GPC3<sub>Apt</sub> complexes formed by the specific reaction between GPC3 and GPC3<sub>Apt</sub> at the sensing interface can cause the sensitive membrane surface potential to change, resulting in the photocurrent-voltage (I-V) curves generate a corresponding offset response. Therefore GPC3 concentration can be determined by monitoring the potential shifts (△V). Under optimal conditions, the potential shift is linearly related to the concentration of GPC3 in the range of 0.001–3.00 μg/mL with the limit of detection (LOD) of 0.0001 μg/mL. The LAPS has a good analytical performance with good specificity, reproducibility and stability, and can be used for the detection of GPC3 in actual serum samples, which provides a broad application prospect for the combined application of LAPS and aptamers in biooassay.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108855"},"PeriodicalIF":4.8,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical immunosensors based on the solubility difference of electroactive probe and the dual signal amplification of nanocarrier plus redox cycling 基于电活性探针溶解度差异和纳米载体加氧化还原循环双重信号放大的电化学免疫传感器

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-26 DOI: 10.1016/j.bioelechem.2024.108858

Yong Chang , Yajun Wang , Xueqian Fan , Jia Zhou , Yunhe Lv , Ning Xia

{"title":"Electrochemical immunosensors based on the solubility difference of electroactive probe and the dual signal amplification of nanocarrier plus redox cycling","authors":"Yong Chang , Yajun Wang , Xueqian Fan , Jia Zhou , Yunhe Lv , Ning Xia","doi":"10.1016/j.bioelechem.2024.108858","DOIUrl":"10.1016/j.bioelechem.2024.108858","url":null,"abstract":"<div><div>This work reported a redox cycling system for the design of electrochemical immunosensors by using pyrroloquinoline quinone (PQQ) to promote the oxidation of tris(2-carboxyethyl)phosphine (TCEP). The consumption of TCEP was monitored with ferrocenium (Fc<sup>+</sup>) as the electroactive probe, which was based on the difference in the solubility of Fc<sup>+</sup> with its reduced format (ferrocene, Fc). Metal–organic framework (MOF) was used as the nanocarrier to load biotinylated recognition antibody and PQQ with recombinant streptavidin as the linker. In the absence of target, TCEP could reduce Fc<sup>+</sup> into insoluble Fc aggregates, thus leading to the decrease in the electrochemical signal. Capture of target allowed for the attachment of antibody-modified MOF-PQQ on the sensing electrode, thus promoting the oxidation of TCEP by O<sub>2</sub> through the redox cycling. In this case, the reduction of Fc<sup>+</sup> into insoluble Fc aggregates was limited, and Fc<sup>+</sup> remained in the solution exhibited a high electrochemical signal. The peak current was linearly proportional to the target concentration in the range of 0.001–1 ng/mL with prostate specific antigen as an example. The work should be useful for the design of novel biosensors through the signal amplification of redox cycling.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108858"},"PeriodicalIF":4.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A DNA sensor based on CbAgo effector protein and on a dual electrochemical signal amplification strategy for B19 parvovirus detection 基于CbAgo效应蛋白和双电化学信号扩增策略的B19细小病毒检测DNA传感器

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-26 DOI: 10.1016/j.bioelechem.2024.108860

Siying Mao , Zhiruo Yang , Zhiyi Liu , Yue Wang , Yonghua Zeng , Nicole Jaffrezic-Renault , Zhipeng Zhang , Yanming Dong , Zhenzhong Guo

{"title":"A DNA sensor based on CbAgo effector protein and on a dual electrochemical signal amplification strategy for B19 parvovirus detection","authors":"Siying Mao , Zhiruo Yang , Zhiyi Liu , Yue Wang , Yonghua Zeng , Nicole Jaffrezic-Renault , Zhipeng Zhang , Yanming Dong , Zhenzhong Guo","doi":"10.1016/j.bioelechem.2024.108860","DOIUrl":"10.1016/j.bioelechem.2024.108860","url":null,"abstract":"<div><div>Human parvovirus B19 is a prevalent childhood infectious virus that poses a great challenge to public health, so the detection of B19V is of great importance. In this study, a DNA sensor based on <em>Cb</em>Ago, a Cas effector, and a dual electrochemical signal amplification strategy was developed by using a novel nanocomposite MnO<sub>2</sub>/CMK-3/g-C<sub>3</sub>N<sub>4</sub>/AgNPs for initial signal amplification, with CMK being an ordered mesoporous carbon nanomaterial. Single-walled carbon nanotubes (SWCNTs) were used as electrocatalytic probes for secondary signal amplification to detect B19 DNA. The detection process begins with polymerase chain reaction (PCR) amplification using the B19V infectious clone plasmid (pB19-M20) as a template and NS1-F/R as primers, followed by specific cleavage of B19 DNA based on the programmable cutting sites of <em>Cb</em>Ago effector protein. This study enriches the application of Argonaute proteins in sensing and introduces a novel method to detect B19V. Under optimized conditions, the biosensor can detect B19 DNA in the range of 10<sup>−15</sup>–10<sup>−10</sup> M, with a detection limit (LOD) of 0.2 fM. The results indicate that the developed DNA sensor holds promise for reliable and sensitive detection of B19 DNA in human serum.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108860"},"PeriodicalIF":4.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel aptamer-based photoelectrochemical sensor for zearalenone detection: Integration of g-C3N4/BiOBr with in situ growth Ag2S quantum dots 一种用于检测玉米赤霉烯酮的基于适配体的新型光电化学传感器：将 g-C3N4/BiOBr 与原位生长的 Ag2S 量子点相结合

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-23 DOI: 10.1016/j.bioelechem.2024.108853

Pengfei Yu , Jiaqi Cui , Jun Yang , Mati Ullah Khan , Lina Yang , Shanshan Li , Meixin Li , Shuang Liu , Ling Kong , Zhiwei Chen

{"title":"A novel aptamer-based photoelectrochemical sensor for zearalenone detection: Integration of g-C3N4/BiOBr with in situ growth Ag2S quantum dots","authors":"Pengfei Yu , Jiaqi Cui , Jun Yang , Mati Ullah Khan , Lina Yang , Shanshan Li , Meixin Li , Shuang Liu , Ling Kong , Zhiwei Chen","doi":"10.1016/j.bioelechem.2024.108853","DOIUrl":"10.1016/j.bioelechem.2024.108853","url":null,"abstract":"<div><div>Zearalenone (ZEN), a secondary metabolite mycotoxin primarily synthesized by <em>Fusarium</em> species and prevalent in cereal grains, exerts estrogenic effects that could induce reproductive toxicity and teratogenic outcomes. To enhance the precision of ZEN detection, we have developed an innovative photoelectrochemical (PEC) aptamer-based sensor employing in situ growth silver sulfide (Ag<sub>2</sub>S) quantum dots-sensitized graphitic carbon nitride/bismuth oxybromide (g-C<sub>3</sub>N<sub>4</sub>/BiOBr) heterojunction. The g-C<sub>3</sub>N<sub>4</sub>/BiOBr composite exhibits robust structural stability and straightforward synthesis. In situ growth Ag<sub>2</sub>S quantum dots could significantly amplify the signal, thereby elevating the sensor’s sensitivity. The sensor utilizes ZEN-specific aptamers for target-specific binding, ensuring high selectivity. Within the concentration range of 0.001 to 100 ng·mL<sup>−1</sup>, the photocurrent response demonstrated a significant linear correlation with the logarithmic scale of ZEN concentration, with a detection limit reaching 0.122 pg·mL<sup>−1</sup>. This PEC aptamer sensor exhibits excellent stability, reproducibility, and selectivity, which is expected to be used as an effective analytical tool for ZEN detection in practical applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108853"},"PeriodicalIF":4.8,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of HPV 16 and 18 L1 genes by a nucleic acid amplification-free electrochemical biosensor powered by CRISPR/Cas9 利用 CRISPR/Cas9 驱动的无核酸扩增电化学生物传感器检测 HPV 16 和 18 L1 基因

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-23 DOI: 10.1016/j.bioelechem.2024.108861

Huynh Vu Nguyen , Seowoo Hwang , Sang Wook Lee , Enjian Jin , Min-Ho Lee

{"title":"Detection of HPV 16 and 18 L1 genes by a nucleic acid amplification-free electrochemical biosensor powered by CRISPR/Cas9","authors":"Huynh Vu Nguyen , Seowoo Hwang , Sang Wook Lee , Enjian Jin , Min-Ho Lee","doi":"10.1016/j.bioelechem.2024.108861","DOIUrl":"10.1016/j.bioelechem.2024.108861","url":null,"abstract":"<div><div>Cervical cancer, closely linked to Human Papillomavirus (HPV) infection, remains a significant health threat for women worldwide. Conventional HPV detection methods, such as reverse transcription polymerase chain reaction (RT-PCR), rely on nucleic acid amplification (NAA), which can be costly and time-consuming. This study introduces an NAA-free electrochemical Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based biosensor designed to detect HPV 16 and HPV 18 L1 genes simultaneously. The system utilizes a Cas9-single guided RNA complex to initiate a selective cleavage reaction, releasing Methylene blue or Ferrocene-labeled fragments correlate to L1 gene concentrations. These fragments then interact with modified gold electrodes immobilized with a complementary probe, allowing precise electrochemical signal measurement during hybridization. The biosensor offers a wide detection range from 1 fM to 10 nM, with detection limits as low as 0.4 fM for HPV 16 L1 and 0.51 fM for HPV 18 L1, providing a sensitive and efficient solution for L1 gene detection. Additionally, its specificity and sensitivity closely match RT-PCR results in clinical testing, highlighting its potential for molecular diagnostics and point-of-care applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108861"},"PeriodicalIF":4.8,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrosion of carbon steel by Pseudomonas stutzeri CQ-Z5 in simulated oilfield water 模拟油田水中的 CQ-Z5 Stutzeri 假单胞菌对碳钢的腐蚀。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-22 DOI: 10.1016/j.bioelechem.2024.108846

Shuang Zhang , Boyu Dong , Dan Zhao , Jiani Yang , Xiufen Sun , Lei Yan

{"title":"Corrosion of carbon steel by Pseudomonas stutzeri CQ-Z5 in simulated oilfield water","authors":"Shuang Zhang , Boyu Dong , Dan Zhao , Jiani Yang , Xiufen Sun , Lei Yan","doi":"10.1016/j.bioelechem.2024.108846","DOIUrl":"10.1016/j.bioelechem.2024.108846","url":null,"abstract":"<div><div>Carbon steel, an important infrastructure material in the petroleum industry, experiences serious damage due to Microbially Influenced Corrosion (MIC) with untold economic impact. <em>Pseudomonas stutzeri</em> CQ-Z5 with solid biofilm formation and organic acid-producing ability was isolated from Changqing oilfield produced water. The corrosion behavior and mechanism of 20<sup>#</sup> carbon steel by <em>P. stutzeri</em> CQ-Z5 were explored in a simulated oilfield product water circulating device. Bacteria inoculation can hasten steel corrosion, the maximum corrosion rate reached 1.84 mm y<sup>−1</sup>. Pitting corrosion on rust layer was observed using SEM, and CLSM monitored the change in biofilm thickness. XRD displayed that oxides were the primary corrosion products, including Fe<sub>2</sub>O<sub>3</sub>, Fe<sub>3</sub>O<sub>4,</sub> and FeOOH. Analysis of contributions of corrosion types indicated that biofilm corrosion contributes 72 % to total corrosion, far higher than those of ion erosion and organic acid decay. Many genes involved in iron metabolism, biofilm synthesis, and organic acid production were annotated in the genome of <em>P. stutzeri</em> CQ-Z5. Accordingly, a hypothetical corrosion mechanism model of <em>P. stutzeri</em> CQ-Z5 for carbon steel involvement of initial ion erosion, then biofilm corrosion and organic acid decay was proposed. The work helped prevent carbon steel corrosion and improve corrosion mitigation strategies.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108846"},"PeriodicalIF":4.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidating different microbiologically influenced corrosion behavior of copper, 90/10 Cu-Ni alloy, 70/30 Cu-Ni alloy and nickel from the perspective of element content 从元素含量的角度阐明微生物对铜、90/10 铜镍合金、70/30 铜镍合金和镍的腐蚀行为的不同影响。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-22 DOI: 10.1016/j.bioelechem.2024.108854

Fan Feng , Yanan Pu , Su Hou , Congrui Zhu , Shougang Chen

{"title":"Elucidating different microbiologically influenced corrosion behavior of copper, 90/10 Cu-Ni alloy, 70/30 Cu-Ni alloy and nickel from the perspective of element content","authors":"Fan Feng , Yanan Pu , Su Hou , Congrui Zhu , Shougang Chen","doi":"10.1016/j.bioelechem.2024.108854","DOIUrl":"10.1016/j.bioelechem.2024.108854","url":null,"abstract":"<div><div>This research examined the varying susceptibility of pure copper (Cu), 90/10 copper-nickel (Cu-Ni) alloy, 70/30 Cu-Ni alloy, and pure nickel (Ni) to microbiologically influenced corrosion (MIC) induced by <em>Desulfovibrio vulgaris</em>, with a focus on the elemental composition of the materials. The results revealed a progressive shift in MIC behavior across these metals and alloys, with increased corrosion severity observed as Ni content decreased. Element Ni improved the corrosion resistance of the alloy while also preventing the growth of microorganisms. Both planktonic and sessile cell counts decreased as the Ni content increased. The corrosion rate, determined by weight loss, followed this order: pure Cu (25.7 ± 3.8 mg·cm<sup>−2</sup>, 0.75 mm·y<sup>−1</sup>) > 90/10 Cu-Ni alloy (9.1 ± 1.4 mg·cm<sup>−2</sup>, 0.27 mm·y<sup>−1</sup>) > 70/30 Cu-Ni alloy (4.3 ± 0.8 mg·cm<sup>−2</sup>, 0.16 mm·y<sup>−1</sup>) > pure Ni (2.1 ± 0.7 mg·cm<sup>−2</sup>, 0.06 mm·y<sup>−1</sup>). The corrosion current density (<em>i</em><sub>corr</sub>) of pure Cu (3.03 × 10<sup>−5</sup> A·cm<sup>−2</sup>) was approximately 20-fold that of pure Ni (1.54 × 10<sup>−6</sup> A·cm<sup>−2</sup>). There was a correlation between the electrochemical and weight loss results. Thermodynamic analysis and experimental results indicated that M-MIC was the primary MIC mechanism for pure Cu. While both M-MIC and EET-MIC were engaged in the MIC mechanisms of 90/10 Cu-Ni and 70/30 Cu-Ni alloys, the predominant mechanism was EET-MIC for pure Ni.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108854"},"PeriodicalIF":4.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel selective and sensitive electrochemical sensor for detection of ovarian cancer biomarker, Cathepsin L 用于检测卵巢癌生物标记物 Cathepsin L 的新型选择性灵敏电化学传感器

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-21 DOI: 10.1016/j.bioelechem.2024.108856

Priya Paliwal , Dignya Desai , Manali Datta

{"title":"Novel selective and sensitive electrochemical sensor for detection of ovarian cancer biomarker, Cathepsin L","authors":"Priya Paliwal , Dignya Desai , Manali Datta","doi":"10.1016/j.bioelechem.2024.108856","DOIUrl":"10.1016/j.bioelechem.2024.108856","url":null,"abstract":"<div><div>Late detection of ovarian cancer is critical as it results in decreased life expectancy of patients. Screening methodology with a rapid turnover is required to ensure better patient outcomes. Till date, no point of care diagnostics exists capable of monitoring epithelial ovarian cancer. Cathepsin L is a classic early-stage biomarker demarcating the proliferation and transition of ovarian cancer. A novel label-free electrochemical diagnostic is proposed for detection of Cathepsin L in serum. Intrinsic fluorescence spectroscopy was used to confirm the interaction between Cathepsin L and probe, Cystatin C. Cystatin C was immobilized on screen-printed carbon electrode using 1-Ethyl-3-(3-dimethyl-aminopropyl carbodiimide and N-hydroxysuccinimide cross linker. Electrochemical fluctuations resulting from Cystatin-Cathepsin interaction was detected by cyclic voltammetry, differential voltammetry and electrochemical impedance spectroscopy within a timeframe of 20 min. The proposed diagnostic for Cathepsin L exhibited analytical performance with a limit of detection upto 70 pg mL<sup>−1</sup> via electrochemical impedance spectroscopy in the dynamic detection range of 2 ng mL<sup>−1</sup>–20 ng mL<sup>−1</sup>. The platform indicated reliable reproducibility, selectivity, and stability. Furthermore, validation with spiked sera samples (n = 5) showed correlation of R<sup>2</sup> = 0.98. The results indicate sensitive yet selective point of care diagnosis of Cathepsin L in serum.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108856"},"PeriodicalIF":4.8,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced corrosion resistance and biofilm inhibition of TC4 with slight Cu addition against marine Pseudomonas aeruginosa 添加少量铜的 TC4 可增强对海洋铜绿假单胞菌的耐腐蚀性和生物膜抑制作用。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-19 DOI: 10.1016/j.bioelechem.2024.108852

Shengchao Yao, Yulin Chen, Xin Zhang, Zhizhong Dong

{"title":"Enhanced corrosion resistance and biofilm inhibition of TC4 with slight Cu addition against marine Pseudomonas aeruginosa","authors":"Shengchao Yao, Yulin Chen, Xin Zhang, Zhizhong Dong","doi":"10.1016/j.bioelechem.2024.108852","DOIUrl":"10.1016/j.bioelechem.2024.108852","url":null,"abstract":"<div><div>Ti-6Al-4V (TC4) alloy is widely utilized as the structural material in marine industries owing to its low density, high specific strength, and favorable corrosion resistance. However, as biofouling drastically alters, some reported the major deleterious effect of bacteria has imposed a challenge to improve microbiologically influenced corrosion (MIC) resistance. A further opportunity for solving this problem is Cu micro-alloying, which was inspired by adding Cu for biomedical applications. Herein, a Ti-6Al-4V alloy with slight Cu addition (TC4-Cu) was exposed to 2216E media inoculated with Pseudomonas aeruginosa (P. A.), and then investigated compared to TC4. TC4-Cu exhibits lower corrosion current, more denser passive film, and lower weight loss with weaker pitting (a maximum pitting depth of 0.2 μm), compared to TC4 with a maximum pitting crater depth of 9.6 μm. Those demonstrated that the presence of Cu significantly improved the MIC resistance, and inhibited the proliferation of P. A., leading to a good antimicrobial efficacy against marine P. A. Moreover, besides the well-known bactericidal role, Cu ions were transferred to form Cu<sub>2</sub>O and CuO, constituting protective corrosion products, and thus improving the anti-microbial properties of TC4-Cu.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108852"},"PeriodicalIF":4.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient bioelectrocatalytic NADH regeneration with a novel amino-functionalized viologen redox polymer 一种新型氨基功能化紫素氧化还原聚合物的高效生物电催化NADH再生

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-11-19 DOI: 10.1016/j.bioelechem.2024.108850

Kavita Jayakumar, Mihai-Cristian Fera, Jose M. Abad, Antonio L. De Lacey, Marcos Pita

{"title":"Efficient bioelectrocatalytic NADH regeneration with a novel amino-functionalized viologen redox polymer","authors":"Kavita Jayakumar, Mihai-Cristian Fera, Jose M. Abad, Antonio L. De Lacey, Marcos Pita","doi":"10.1016/j.bioelechem.2024.108850","DOIUrl":"10.1016/j.bioelechem.2024.108850","url":null,"abstract":"<div><div>Oxidoreductase enzymes, used for a variety of applications including organic synthesis and pharmaceutical industry, require reduced nicotinamide adenine dinucleotide (NADH) as reducing equivalents. Methods for regenerating NAD<sup>+</sup> to NADH are of significant interest due to the high cost and stoichiometric amounts of cofactor required. Diaphorase/redox mediator systems have shown promise for this purpose, but suitable mediators are few due to the low redox potential required, necessary downstream processing and stability issues. A novel amino-functionalized viologen is presented in this work which, upon immobilization with diaphorase, yields bioactive NADH with high selectivity (99 %) and faradaic efficiency (99 %). This system was tested with NADH-dependent formate dehydrogenase, showing a 21-fold improvement in formate yield compared to an enzymatic negative control without NADH regeneration. The findings underscore the potential of this novel amino-functionalized viologen polymer to advance sustainable and efficient NADH regeneration at very low overpotential.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"162 ","pages":"Article 108850"},"PeriodicalIF":4.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0