Bioelectrochemistry最新文献_第5页

Bidirectional electro-enzymatic reaction of coenzyme F420 using benzyl viologen and F420-dependent sulfite reductase

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-28 DOI: 10.1016/j.bioelechem.2025.108922

Satoshi Furota , Masanori Kaneko , Seiya Tsujimura , Daijiro Takeshita , Yusuke Nakamichi , Kensuke Igarashi , Masaru K. Nobu , Miho Yoshikawa , Kenta Asahina , Chie Fukaya , Toshie Ishitsuka , Kazuma Shimada

{"title":"Bidirectional electro-enzymatic reaction of coenzyme F420 using benzyl viologen and F420-dependent sulfite reductase","authors":"Satoshi Furota , Masanori Kaneko , Seiya Tsujimura , Daijiro Takeshita , Yusuke Nakamichi , Kensuke Igarashi , Masaru K. Nobu , Miho Yoshikawa , Kenta Asahina , Chie Fukaya , Toshie Ishitsuka , Kazuma Shimada","doi":"10.1016/j.bioelechem.2025.108922","DOIUrl":"10.1016/j.bioelechem.2025.108922","url":null,"abstract":"<div><div>Coenzyme F<sub>420</sub> is recognized as a crucial electron carrier in methane-generating metabolism but, beyond this, has garnered significant attention for its role in diverse microbial physiologies and relevance in industrial, medical, and environmental applications. However, one limitation of current application of F<sub>420</sub> is the necessity of chemical electron donors for its reduction. In this study, an electrochemical reaction system was designed to facilitate electron transfer between the electrode and F<sub>420</sub> using F<sub>420</sub>-dependent sulfite reductase (Fsr) as the catalyst and benzyl viologen (BV) as the redox mediator. Photometric analysis and cyclic potential scanning demonstrated that the occurrence of bidirectional (reversible) electrochemical oxidation and reduction of F<sub>420</sub> in this system depended on the electrode potential. The formal redox potential of F<sub>420</sub> in this system was −540 mV vs. Ag|AgCl|sat. KCl, which aligned with values previously determined using biochemical assays.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108922"},"PeriodicalIF":4.8,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of boron doped diamond electrodes with engineered sp2 carbon content and their application to structure-dependent DNA hybridization

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-27 DOI: 10.1016/j.bioelechem.2025.108910

Ondrej Hesko , Hana Pivoňková , Lukáš Fojt , Andrew Taylor , Jaromír Kopeček , Karolina Schwarzová-Pecková , Miroslav Fojta

{"title":"Characterization of boron doped diamond electrodes with engineered sp2 carbon content and their application to structure-dependent DNA hybridization","authors":"Ondrej Hesko , Hana Pivoňková , Lukáš Fojt , Andrew Taylor , Jaromír Kopeček , Karolina Schwarzová-Pecková , Miroslav Fojta","doi":"10.1016/j.bioelechem.2025.108910","DOIUrl":"10.1016/j.bioelechem.2025.108910","url":null,"abstract":"<div><div>Boron doped diamond electrodes brought a new potential in bioanalytical chemistry including studies of structure and interactions of nucleic acids. Herein, deposition conditionswere optimized to produce a set of polycrystalline BDD electrodes with comparable boron concentration in solid phase of (1.8 − 2.1) · 10<sup>21</sup> cm<sup>−3</sup> akin to metallic-type conductivity but with increasing sp<sup>2</sup>   300 nm (BDD<sub>0.25</sub>) to < 100 nm (BDD<sub>2.0</sub>). Adsorption of oligodeoxynucleotides and their structural changes in the presence of K<sup>+</sup> and Li<sup>+</sup> ions were evaluated through enzyme-linked DNA hybridization assay in which oxidizable 1-naphthol was released from its phosphoesterbystreptavidin–alkaline phosphatase conjugate upon successful hybridization of the target oligodeoxynucleotide with a biotinylated complementary probe. With increasing sp<sup>2</sup> ions that don’t stabilize the quadruplex structure. Such behaviour was observed also for commercial BDD electrode with surface roughness < 10 nm.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108910"},"PeriodicalIF":4.8,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143155964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-free electrochemical immunosensors based on Cu-Ni metal–organic framework and carbon nanotube composite for carcinoembryonic antigen detection

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-27 DOI: 10.1016/j.bioelechem.2025.108918

Shi Wang , Jialin Ao , Saiwen Ding , Ting Shu

引用次数: 0

Ferredoxin NADP+ reductase for NADPH and NADH regeneration in a flow bioelectrochemical reactor

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-27 DOI: 10.1016/j.bioelechem.2025.108919

Wassim El Housseini , François Lapicque , Alain Walcarius , Elisabeth Lojou , Nicolas Rouhier , Mathieu Etienne

{"title":"Ferredoxin NADP+ reductase for NADPH and NADH regeneration in a flow bioelectrochemical reactor","authors":"Wassim El Housseini , François Lapicque , Alain Walcarius , Elisabeth Lojou , Nicolas Rouhier , Mathieu Etienne","doi":"10.1016/j.bioelechem.2025.108919","DOIUrl":"10.1016/j.bioelechem.2025.108919","url":null,"abstract":"<div><div>Ferredoxin-NADP<sup>+</sup> reductase (FNR) is an efficient and selective biocatalyst to continuously regenerate the NADPH cofactor consumed in biomolecular synthesis for the chemical and pharmaceutical sectors. In this work, FNR from <em>Chlamydomonas reinhardtii</em> was applied to electrochemical regeneration of the nicotinamide cofactors, by combining this enzymatic catalyst in a flow reactor with the oxidation of hydrogen, a clean source of electrons and protons. FNR was immobilized on the surface of oxidized multi-walled carbon nanotubes, which allowed maintaining its activity for over six days under high flow rate. Surprisingly, this modified FNR electrode was effective not only in regenerating NADPH but also NADH. The cofactor regeneration was then applied to the NADH-dependent production of lactate from pyruvate, using L-lactate dehydrogenase (LDH) in the presence of low NAD<sup>+</sup> concentration (10 µM). Both FNR and LDH enzymes were immobilized in the bioelectrochemical system that achieved a remarkable total turnover number (TTN) of 10<sup>4</sup> for the nicotinamide cofactor and a faradaic efficiency higher than 80 %.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108919"},"PeriodicalIF":4.8,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143155965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the electrochemical behaviour of digestive enzymes at a liquid|liquid micro-interface array

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-26 DOI: 10.1016/j.bioelechem.2025.108911

Shaheda Zannah, Damien W.M. Arrigan

{"title":"Exploring the electrochemical behaviour of digestive enzymes at a liquid|liquid micro-interface array","authors":"Shaheda Zannah, Damien W.M. Arrigan","doi":"10.1016/j.bioelechem.2025.108911","DOIUrl":"10.1016/j.bioelechem.2025.108911","url":null,"abstract":"<div><div>Trypsin and pepsin are proteolytic enzymes secreted by the digestive system to digest proteins. Here, we examine the electrochemical behaviour and detection of trypsin and pepsin at a liquid/liquid (L|L) micro-interface array. For both proteins, aqueous phase of 10 mM hydrochloric acid was the only electrolyte solution in which they were electroactive. Neither protein was detected below 30 μM by cyclic voltammetry (CV) but stripping voltammetry following adsorption (AdSV) enabled the detection of sub-micromolar concentrations of both proteins. Although pepsin was electroactive at the micro-interface array in aqueous phase of 10 mM HCl, its behaviour was ill-defined and unsuitable for characterization by CV. It was found that pepsin easily blocked the micro-interfaces, as seen by greatly hampered ion transfer voltammetry of tetrapropylammonium ion (TPrA<sup>+</sup>) whereas trypsin only slightly impeded TPrA<sup>+</sup> transfer. This highlights the dissimilarity between pepsin and trypsin. These results illustrate the rich viability of electrochemistry at L|L micro-interface arrays as a tool to explore the behaviour and detection of biological macromolecules.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108911"},"PeriodicalIF":4.8,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paper-based electrochemiluminescence telomerase activity detection using hybridization chain reaction and CRISPR/Cas12a dual signal amplification

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-26 DOI: 10.1016/j.bioelechem.2025.108916

Yun Zhang , Liang Gao , Zhe Shi , Qiong Wu , Xiangmin Miao

{"title":"Paper-based electrochemiluminescence telomerase activity detection using hybridization chain reaction and CRISPR/Cas12a dual signal amplification","authors":"Yun Zhang , Liang Gao , Zhe Shi , Qiong Wu , Xiangmin Miao","doi":"10.1016/j.bioelechem.2025.108916","DOIUrl":"10.1016/j.bioelechem.2025.108916","url":null,"abstract":"<div><div>Sensitive telomerase activity detection becomes particularly significance since the important value of it in early cancer diagnosis as a potential biomarker. Herein, we developed a paper-based analytical devices (PADs) for telomerase activity detection, using positively charged Au@luminol nanoparticles ((+)Au@luminol NPs) as electrochemiluminescence (ECL) signal probe coupling with hybridization chain reaction (HCR) and CRISPR/Cas12a dual signal amplification. Firstly, the initial strong ECL signal was obtained based on the electrostatic adsorption of (+)Au@luminol NPs onto the surface of HCR double-stranded hybrid aggregates. In the presence of telomerase, the primer was efficiently elongated with telomeric repeats of (TTAGGG)<sub>n</sub> to release activator DNA and trigger the CRISPR/Cas12a, which can prevent the happen of HCR and the adsorption of (+)Au@luminol NPs through cleaving the capture probe on the electrode surface, such results directly inducing the decrease of the ECL signal that was proportional to telomerase concentration, due to the efficient signal amplification of HCR and CRISPR/Cas12a, a low detection limit of 2.3 cells/mL for telomerase could be detected. Moreover, the sensor realized the effective application for telomerase extracts analysis in human serum samples, making it possess potential application value for telomerase activity assays in cancer diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108916"},"PeriodicalIF":4.8,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143155422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrochemiluminescence strategy with proximity ligation triggered multiple catalytic hairpin assembly induced CRISPR/Cas 12a system for analysis of paraquat

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-25 DOI: 10.1016/j.bioelechem.2025.108915

Wen-Jie Zhong , Wei-Guo Yang , Ying Zhang , Tao Li , Ming-Li Su , Ruo Yuan , Shangcheng Xu , Wen-Bin Liang

{"title":"An electrochemiluminescence strategy with proximity ligation triggered multiple catalytic hairpin assembly induced CRISPR/Cas 12a system for analysis of paraquat","authors":"Wen-Jie Zhong , Wei-Guo Yang , Ying Zhang , Tao Li , Ming-Li Su , Ruo Yuan , Shangcheng Xu , Wen-Bin Liang","doi":"10.1016/j.bioelechem.2025.108915","DOIUrl":"10.1016/j.bioelechem.2025.108915","url":null,"abstract":"<div><div>Paraquat (PQ) as a widely used non-selective herbicides has gained attention in agricultural residue detection and food safety. Herein, a novel quantitative analysis approach for PQ was proposed based on a novel kind of aggregation-induced emission electrochemiluminescence (AIECL) emitters, tetraphenylethylene-luminol (TPE-L) with a small molecule-induced multiple catalytic hairpin assembly (CHA) amplification strategy, the competitive immune reaction and CRISPR/Cas12a system. The target molecule PQ is introduced into a signal cycle, and auxiliary sensitization cycles are constructed by virtue of the cleavage characteristics of the CRISPR/Cas12a system, which realized the multiple utilization of the target by using both cis- and <em>trans</em>-cleavage activities. In addition, the new multiple CHA amplification strategy was attributed to cross-catalytic hairpin assembly caused by the products of the CHA cycle as the initiator chain of the next CHA cycle, realizing the efficient utilization of cyclic products and producing high-efficiency signal amplification. Thus, the ECL biosensor for ultrasensitive analysis of PQ was successfully constructed with a limit of detection of 0.7 pg/mL. Importantly, it could be easily-extended to other small molecules simply by replacing paired antibodies, providing prospects in agricultural residue detection, food safety and related medical applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"164 ","pages":"Article 108915"},"PeriodicalIF":4.8,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143155967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced cortisol detection: A cMWCNTs-enhanced MB@Zr-MOF ratiometric electrochemical aptasensor

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-23 DOI: 10.1016/j.bioelechem.2025.108914

Runran Ma , Te Li , Xueying Li , Jianwei Han , Xin Zhang , Tingting Di , Jiabo Wang , Weijun Kong

{"title":"Advanced cortisol detection: A cMWCNTs-enhanced MB@Zr-MOF ratiometric electrochemical aptasensor","authors":"Runran Ma , Te Li , Xueying Li , Jianwei Han , Xin Zhang , Tingting Di , Jiabo Wang , Weijun Kong","doi":"10.1016/j.bioelechem.2025.108914","DOIUrl":"10.1016/j.bioelechem.2025.108914","url":null,"abstract":"<div><div>A ratiometric electrochemical aptasensor was developed for ultra-sensitive detection of cortisol using aptamer (Apt) as recognition element, methylene blue (MB) as signal probe, and zirconium metal–organic framework (Zr-MOF) as carrier loaded with abundant MB for signal amplification. The carboxylated multi-walled carbon nanotubes (cMWCNTs)-modified Au electrode showed excellent electrochemical performance to immobilize complementary DNA (cDNA) for hybridizing with MB@Zr-MOF-Apt <em>via</em> amide bonds. In the presence of cortisol, it would compete with cDNA for binding the Apt, resulting in the detachment of MB@Zr-MOF-Apt complex from the electrode surface, and the electrochemical signal of MB was decreased, while that of [Fe(CN)<sub>6</sub>]<sup>3−/4−</sup> was basically unchanged. The ratio of the electrochemical signals of [Fe(CN)<sub>6</sub>]<sup>3−/4−</sup> to MB was proportional to the cortisol concentration. Due to the greatly enhanced conductivity of the cMWCNTs-decorated Au electrode and the largely improved EC signals of Zr-MOF encapsulated MB probes, this ratiometric electrochemical aptasensor offered high sensitivity with an ultra-low detection limit of 0.0046 nM and a wide linearity of 0.01–1000 nM, as well as satisfactory accuracy with recoveries of 93.79–106.76 % in artificial sweat samples, providing a potential strategy for the detection of more trace hormones in different clinical samples by simply replacing the corresponding aptamers.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108914"},"PeriodicalIF":4.8,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrochemical aptasensor based on bimetallic carbon nanocomposites AuPt@rGO for ultrasensitive detection of adenosine on portable potentiostat

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-22 DOI: 10.1016/j.bioelechem.2025.108906

Zheng Gong , Hongli Zhao , Yi Mao , Fangfang Zhou , Zehui Shi , Minbo Lan

{"title":"An electrochemical aptasensor based on bimetallic carbon nanocomposites AuPt@rGO for ultrasensitive detection of adenosine on portable potentiostat","authors":"Zheng Gong , Hongli Zhao , Yi Mao , Fangfang Zhou , Zehui Shi , Minbo Lan","doi":"10.1016/j.bioelechem.2025.108906","DOIUrl":"10.1016/j.bioelechem.2025.108906","url":null,"abstract":"<div><div>Adenosine plays a crucial role in the cardiovascular and nervous systems of living organisms. Excessive adenosine can lead to arrhythmias or heart failure, making the accurate detection of adenosine highly valuable. Given the widespread use of sensors for detecting small molecules, we propose a sensitive electrochemical aptasensor for adenosine detection in this study. The aptasensor utilized reduced graphene oxide (rGO) as the base material, and its performance was further enhanced by loading gold-platinum nanoparticles (AuPt@rGO) to improve the current response. The AuPt@rGO composite was synthesized using a simple process and was modified onto screen-printed electrodes (SPE) fabricated in the laboratory. This modification increased the effective area of the working electrode, providing more contact sites and enhancing sensitivity for adenosine detection. As a result, the sensor exhibited excellent performance in selectivity, sensitivity, and stability, with a linear response range for adenosine from 10 nM to 10 μM and a detection limit of 1.28 nM (n = 3). Furthermore, successful detection of adenosine levels in real samples was achieved using a portable potentiostat, demonstrating excellent recovery rates (95.99 %–103.4 %). These results indicate the aptasensor’s significant potential for future applications in field analysis and medical diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108906"},"PeriodicalIF":4.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytochrome P450 electrochemical biosensors transforming in vitro metabolism testing – Opportunities and challenges

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-21 DOI: 10.1016/j.bioelechem.2025.108913

Carina S.P. Vieira, Marcela A. Segundo, Alberto N. Araújo

{"title":"Cytochrome P450 electrochemical biosensors transforming in vitro metabolism testing – Opportunities and challenges","authors":"Carina S.P. Vieira, Marcela A. Segundo, Alberto N. Araújo","doi":"10.1016/j.bioelechem.2025.108913","DOIUrl":"10.1016/j.bioelechem.2025.108913","url":null,"abstract":"<div><div>The ability of the living world to flourish in the face of constant exposure to dangerous chemicals depends on the management ability of a widespread group of enzymes known as heme-thiolate monooxygenases or cytochrome P450 superfamily. About three-quarters of all reactions determining the metabolism of endogenous compounds, of those carried in foods, of taken drugs, or even of synthetic chemicals discarded into the environment depend on their catalytic performance. The chromatographic and (photo)luminometric methods routinely used as predictive and analytical tools in laboratories have significant drawbacks ranging from limited shelf-life of reagents, use of synthetic substrates, laborious and tedious procedures for highly sensitive detection. In this review, alternative electrochemical biosensors using the cytochrome P450 enzymes as bio-element are emphasized in their main aspects as well regarding their implementation and usefulness. Despite the various schemes proposed for the implementation, reports on real applications are scant for several reasons, including low reaction rates, broad substrate specificity, uncoupling reactions occurrence, and the need for expensive electron transfer partners to promote electron transfer. Finally, the prospect for future developments is introduced, focusing on integrating miniaturized systems with electrochemical techniques, alongside optimizing enzyme immobilization methods and electrode modifications to improve enzymatic stability and enhance sensor reliability. This progress represents a crucial step towards the creation of portable biosensors that mimic human physiological responses, supporting the precision medicine approach.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108913"},"PeriodicalIF":4.8,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0