Integrated photoelectrochemical/photothermal dual-mode biosensor with simultaneous dual-signal output for detection of bladder cancer cells HT-1376

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-08-06 DOI:10.1016/j.bioelechem.2025.109075

Qingqing Zhang , Tingting Zhai , Jie Chen , Zhongxiu Chen , Hao Tan , Lifen Long , Tingting Hao , Zhiyong Guo

{"title":"Integrated photoelectrochemical/photothermal dual-mode biosensor with simultaneous dual-signal output for detection of bladder cancer cells HT-1376","authors":"Qingqing Zhang , Tingting Zhai , Jie Chen , Zhongxiu Chen , Hao Tan , Lifen Long , Tingting Hao , Zhiyong Guo","doi":"10.1016/j.bioelechem.2025.109075","DOIUrl":null,"url":null,"abstract":"<div><div>Here, a photoelectrochemical (PEC)/photothermal (PT) dual-mode biosensing platform with simultaneous output of response signals was developed for sensitive and accurate detection of bladder tumor cells HT-1376. The system utilized an infrared laser as a shared light source, with photocurrent and temperature measurements acquired through parallel PEC and PT detection channels. The HT-1376-specific aptamer (Apt) was covalently immobilized on aminated indium‑tin oxide (ITO) electrode via glutaraldehyde crosslinking between surface amino groups and amine-modified aptamer terminals, enabling selective target capture. Following aptamer-HT-1376 recognition, the dual-mode tags CuO@NPC functionalized with aptamer (CuO@NPC-Apt) were assembled on the electrode surface, triggering simultaneous PEC and PT signal responses. Under optimized conditions, linear detection ranges of 5–10<sup>5</sup> cells/mL (PEC mode) and 10–10<sup>5</sup> cells/mL (PT mode) were achieved, with limits of detection (LOD) of 3 cells/mL (PEC) and 5 cells/mL (PT). The platform exhibited excellent signal stability, repeatability and selectivity. This dual-mode strategy provides a reliable analytical method for HT-1376 quantification, demonstrating potential for clinical cancer diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"Article 109075"},"PeriodicalIF":4.5000,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioelectrochemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567539425001781","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Here, a photoelectrochemical (PEC)/photothermal (PT) dual-mode biosensing platform with simultaneous output of response signals was developed for sensitive and accurate detection of bladder tumor cells HT-1376. The system utilized an infrared laser as a shared light source, with photocurrent and temperature measurements acquired through parallel PEC and PT detection channels. The HT-1376-specific aptamer (Apt) was covalently immobilized on aminated indium‑tin oxide (ITO) electrode via glutaraldehyde crosslinking between surface amino groups and amine-modified aptamer terminals, enabling selective target capture. Following aptamer-HT-1376 recognition, the dual-mode tags CuO@NPC functionalized with aptamer (CuO@NPC-Apt) were assembled on the electrode surface, triggering simultaneous PEC and PT signal responses. Under optimized conditions, linear detection ranges of 5–10⁵ cells/mL (PEC mode) and 10–10⁵ cells/mL (PT mode) were achieved, with limits of detection (LOD) of 3 cells/mL (PEC) and 5 cells/mL (PT). The platform exhibited excellent signal stability, repeatability and selectivity. This dual-mode strategy provides a reliable analytical method for HT-1376 quantification, demonstrating potential for clinical cancer diagnostics.

查看原文本刊更多论文

同时双信号输出的集成光电化学/光热双模生物传感器用于膀胱癌细胞HT-1376的检测

本研究建立了同时输出响应信号的光电化学(PEC)/光热（PT）双模生物传感平台，对膀胱癌细胞HT-1376进行灵敏、准确的检测。该系统利用红外激光器作为共享光源，通过平行的PEC和PT检测通道获得光电流和温度测量。将ht -1376特异性适配体（Apt）通过表面氨基与胺修饰适配体末端之间的戊二醛交联共价固定在胺化氧化铟锡（ITO）电极上，实现选择性靶捕获。在对适配体- ht -1376进行识别后，将适配体（CuO@NPC-Apt）功能化的双模标签CuO@NPC组装在电极表面，同时触发PEC和PT信号响应。在优化条件下，线性检测范围为5 ~ 105 cells/mL （PEC模式）和10 ~ 105 cells/mL （PT模式），检出限（LOD）分别为3 cells/mL （PEC）和5 cells/mL （PT）。该平台具有良好的信号稳定性、重复性和选择性。这种双模式策略为HT-1376定量提供了可靠的分析方法，显示了临床癌症诊断的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.