Plasma jet and plasma treated aerosol induced permeation of reconstructed human epidermis

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-08-05 DOI:10.1016/j.bioelechem.2025.109060

Vinodini Vijayarangan , Zeineb Maaroufi , Amaury Rouillard , Septuce Gaetan-Zin , Sébastien Dozias , Pablo Escot-Bocanegra , Augusto Stancampiano , Catherine Grillon , Eric Robert

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引用次数: 0

Abstract

As plasma-treated liquids have many applications in plasma medicine, their cutaneous effects for cosmetic purposes are also considered as an alternative way to treat skin without the electric hazards and limitations correlated with the use of a direct plasma. Our previous work on human skin explants showed increased transdermal diffusion of cosmetic ingredients (caffeine, hyaluronic acid) after direct plasma treatment. Despite this proven efficacy, these protocols still face limitations dealing with toxicity, small treatment areas and uneven surface coverage. To overcome these limitations and broaden the scope of non-thermal-plasma-based technology for skin care, this study presents for the first time the development and assessment of a plasma aerosol device to nebulize plasma-treated liquids on skin models.

This work demonstrates how plasma jet and plasma treated aerosol can temporarily enhance permeation in reconstructed human epidermis (RHE), using fluorescein as a probe, under safe plasma delivery conditions. Transepithelial electrical resistance measurements confirm the transient nature of the plasma-induced modulation, suggesting the possibility to control the duration of the enhanced permeation. Overall, the achieved results demonstrate the potential of plasma jet and plasma treated aerosol to safely control diffusion through skin for cosmetic and medical purposes.

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等离子体喷射和等离子体处理的气溶胶诱导的人体重建表皮的渗透

由于等离子体处理的液体在等离子体医学中有许多应用，其美容目的的皮肤效果也被认为是治疗皮肤的另一种方法，没有与使用直接等离子体相关的电危害和限制。我们之前对人体皮肤外植体的研究表明，直接血浆处理后，化妆品成分（咖啡因、透明质酸）的透皮扩散增加。尽管这些方案被证明是有效的，但在处理毒性、治疗区域小和表面覆盖不均匀等问题时，这些方案仍然面临局限性。为了克服这些限制并扩大非热等离子体皮肤护理技术的范围，本研究首次提出了一种等离子体气溶胶装置的开发和评估，用于在皮肤模型上雾化等离子体处理过的液体。这项工作展示了等离子体喷射和等离子体处理的气溶胶如何在安全的等离子体输送条件下，使用荧光素作为探针，暂时增强重建人体表皮（RHE）的渗透性。经上皮电阻测量证实了等离子体诱导调制的瞬态性质，表明有可能控制增强渗透的持续时间。总的来说，取得的结果表明，等离子体射流和等离子体处理气溶胶的潜力，以安全控制扩散通过皮肤美容和医疗目的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.