Bioelectrochemistry最新文献_第4页

Effect of Ni content in CuNi alloys on the repassivation behavior of passive films by Pseudomonas aeruginosa and Desulfovibrio vulgaris cu合金中Ni含量对铜绿假单胞菌和普通脱硫弧菌钝化膜再钝化行为的影响

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-08-27 DOI: 10.1016/j.bioelechem.2025.109096

Chengjiao Jiao, Xueyan Shan, Min Du, Gengxin Li

{"title":"Effect of Ni content in CuNi alloys on the repassivation behavior of passive films by Pseudomonas aeruginosa and Desulfovibrio vulgaris","authors":"Chengjiao Jiao, Xueyan Shan, Min Du, Gengxin Li","doi":"10.1016/j.bioelechem.2025.109096","DOIUrl":"10.1016/j.bioelechem.2025.109096","url":null,"abstract":"<div><div>A study employed surface morphology observation, corrosion product composition analysis, and electrochemical measurements to investigate differences in passive film breakdown and repassivation processes between pre-passivated B10 and B30 Cu<img>Ni alloys under synergistic corrosion by <em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa</em>) and <em>Desulfovibrio vulgaris</em> (<em>D. vulgaris</em>) in seawater, attributable to their differing Ni content. The results show that the growth-metabolic activity of <em>P. aeruginosa</em> and <em>D. vulgaris</em> accelerated the selective dissolution of surface nickel on the alloy. Higher Ni content in the alloys corresponded to increased proportions of Cu<sub>2</sub>O and NiO in the pre-formed passive film, resulting in a denser passive layer that provided superior short-term protective properties, manifested by higher total electrochemical impedance and slower pitting corrosion rates. However, these dense film limited the migration of Ni<sup>2+</sup>, resulting in the obstruction of the repair of NiO in the middle and late stages, the poor protection of the passive film and the faster pitting rate. This study indicates that in the seawater environment where <em>P. aeruginosa</em> and <em>D. vulgaris</em> coexist, the pre-passivated Cu<img>Ni alloy with low Ni content has stronger passive film repair ability and better corrosion resistance in the middle and late stages of immersion.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"Article 109096"},"PeriodicalIF":4.5,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aerobic mild bioelectrocatalysis: Disentangling dual redox pathways for H2 evolution amidst competing oxygen reduction in S. cerevisiae biofilm 好氧温和生物电催化：在酿酒酵母生物膜的竞争性氧还原中，解开H2进化的双氧化还原途径

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-08-26 DOI: 10.1016/j.bioelechem.2025.109093

Graziela C. Sedenho , Rodrigo M. Iost , Rafael L. Romano , Maykon L. Souza , Fabio H.B. de Lima , Frank N. Crespilho

引用次数: 0

The assessment of interaction kinetics between specific monoclonal antibody and immobilized SARS-CoV-2 nucleoprotein 特异性单克隆抗体与固定化SARS-CoV-2核蛋白相互作用动力学评价

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-08-25 DOI: 10.1016/j.bioelechem.2025.109090

Yana Karnitskaya , Maryia Drobysh , Almira Ramanaviciene , Agne Rimkute , Indre Kucinskaite-Kodze , Greta Zvirzdine , Rimantas Slibinskas , Oksana Pogorielova , Viktoriia Korniienko , Arunas Ramanavicius

{"title":"The assessment of interaction kinetics between specific monoclonal antibody and immobilized SARS-CoV-2 nucleoprotein","authors":"Yana Karnitskaya , Maryia Drobysh , Almira Ramanaviciene , Agne Rimkute , Indre Kucinskaite-Kodze , Greta Zvirzdine , Rimantas Slibinskas , Oksana Pogorielova , Viktoriia Korniienko , Arunas Ramanavicius","doi":"10.1016/j.bioelechem.2025.109090","DOIUrl":"10.1016/j.bioelechem.2025.109090","url":null,"abstract":"<div><div>The emergence of SARS-CoV-2 has posed significant global health challenges. The nucleocapsid protein (N-protein) is a structural part of the SARS-CoV-2 virus and an important immunogenic target of specific antibodies, which are developed in the organism during the infection by this virus. Artificially designed specific (monoclonal and polyclonal) antibodies are also used for therapeutic and bioanalytical purposes, therefore, the assessment and characterization of newly designed antibodies is an important analytical issue. This study reports an electrochemical biosensing system for the assessment of the interaction between newly designed specific antibody and SARS-CoV-2 recombinant N-protein, against which the antibody was designed, and which was used as a target in biological recognition part of the biosensing system. The biosensing system was applied for the determination of the interaction kinetics between immobilized N-protein and a newly derived monoclonal antibody (mAb) 16D9 (mAb-16D9). Cyclic voltammetry was employed to evaluate the kinetics of the interaction between the recombinant N-protein and mAb-16D9. The binding constant (<em>K</em>c) was determined to be 50.99 μg/mL, demonstrating a strong affinity, while the limit of detection was 4.3 × 10<sup>−4</sup> μg/mL, highlighting sufficient affinity of mAb-16D9 towards N-protein, which determined good sensitivity of the developed biosensors. These findings highlight the potential application of the here-reported electrochemical biosensor for rapid testing of antigen-antibody interaction kinetics and the characterization of newly designed antibody.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109090"},"PeriodicalIF":4.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Liquid-phase epitaxial grown UiO-66 as a bifunctional platform for electrochemical Aptasensing of cardiac troponin I 液相外延生长UiO-66作为电化学偶联肌钙蛋白I的双功能平台

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-08-25 DOI: 10.1016/j.bioelechem.2025.109091

Yuan Zhu, Sifan Wang, Haizhu Yang, Feng Gao, Qingxiang Wang

{"title":"Liquid-phase epitaxial grown UiO-66 as a bifunctional platform for electrochemical Aptasensing of cardiac troponin I","authors":"Yuan Zhu, Sifan Wang, Haizhu Yang, Feng Gao, Qingxiang Wang","doi":"10.1016/j.bioelechem.2025.109091","DOIUrl":"10.1016/j.bioelechem.2025.109091","url":null,"abstract":"<div><div>The construction of multifunctional nanointerface bearing rich active site for aptamer immobilization and effective signal transformation is critical for the development of high-performance electrochemical aptasensor. Herein, a layer of <em>p</em>-mercaptobenzoic acid (<em>p</em>-MBA) was self-assembled on the surface of bare gold electrode (AuE), acting as a scalfod for the consequent liquid-phase epitaxial growth of Universitetet i Oslo-66 (UiO-66) metal-organic framework via immersion in zirconium ions (Zr<sup>4+</sup>) and <em>p</em>-terephthalic acid (PTA) ligand. Then, the UiO-66 was utilized as a bifunctional platform for immobilization of aptamer probe for cardiac troponin I (cTnI) and the electrochemical signal transformation as an electrocatalyst. Electrochemical experiments showed that UiO-66 has catalase-like properties for electrocatalytic reduction of H<sub>2</sub>O<sub>2</sub>, while when the aptamer interacts with cTnI to form a complex, the catalytic acttivity is inhibited. The catalytic currents in chronoamperometric measurements showed a good linear relationship with the negative logarithm of the target concentration in the range of 100 fg mL<sup>−1</sup>–100 ng mL<sup>−1</sup>, and the detection limit was 13 fg mL<sup>−1</sup>. The aptasensor has good selectivity for cTnI and can be used for the detection of cTnI in human serum samples.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"Article 109091"},"PeriodicalIF":4.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144906854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Deciphering gold nanotag-induced electron-transfer blockage in competitive electrochemical immunosensor: toward a nanoparticle-sandwiched structure for sensitive SARS-CoV-2 antigen detection 解读竞争性电化学免疫传感器中金纳米标签诱导的电子转移阻塞：面向敏感的SARS-CoV-2抗原检测的纳米颗粒夹层结构

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-08-25 DOI: 10.1016/j.bioelechem.2025.109092

Patrawadee Yaiwong , Siriporn Anuthum , Jaturong Khonghuayrob , Nuttagamon Thiangem , Jaroon Jakmunee , Kontad Ounnunkad

{"title":"Deciphering gold nanotag-induced electron-transfer blockage in competitive electrochemical immunosensor: toward a nanoparticle-sandwiched structure for sensitive SARS-CoV-2 antigen detection","authors":"Patrawadee Yaiwong , Siriporn Anuthum , Jaturong Khonghuayrob , Nuttagamon Thiangem , Jaroon Jakmunee , Kontad Ounnunkad","doi":"10.1016/j.bioelechem.2025.109092","DOIUrl":"10.1016/j.bioelechem.2025.109092","url":null,"abstract":"<div><div>Rapid and sensitive detection of SARS-CoV-2 remains essential for infection control and pandemic preparedness, especially with the continuous emergence of new variants. In this study, a competitive electrochemical immunosensor was developed for the detection of the S-protein using a polyethyleneimine-coated gold nanoparticle (PEI-AuNP)-based strategy. The screen-printed carbon electrode (SPCE) was modified with PEI-AuNPs to enhance surface area, conductivity, and biocompatibility, allowing efficient immobilization of the S-protein. Simultaneously, PEI-AuNPs conjugated with anti-S-protein antibodies (anti-S-protein@PEI-AuNPs) were used as nanotags. In the presence of the target antigen, antigen-antibody complexes form in the solution, reducing nanotag binding to the electrode. In contrast, the absence of the target leads to a higher density of nanotags on the electrode surface, which hinders electron transfer of the redox probe [Fe(CN)<sub>6</sub>]<sup>3−/4–</sup>, resulting in a lower current response. The sensor demonstrated a wide linear detection range from 0.10 to 25 ng mL<sup>−1</sup>, with a low detection limit of 0.050 ng mL<sup>−1</sup>. It exhibited excellent sensitivity, selectivity, and stability. Successful detection in human serum, indoor surface swabs, and tap water with high recovery highlights its real-world applicability. This platform can be adapted for emerging pathogens, making it a valuable tool for current and future pandemic surveillance.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"Article 109092"},"PeriodicalIF":4.5,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144904467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing fibroblast-based bone regeneration by harnessing nanosecond pulsed electric field 利用纳秒脉冲电场增强成纤维细胞骨再生

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-08-22 DOI: 10.1016/j.bioelechem.2025.109089

Tian Tu , Chenguang Ouyang , Pengfei Li , Zhipeng Ni , Zewei Wang , Jingtian Lai , Xinhua Chen , Zhenfeng Liu , Hui Lu

{"title":"Enhancing fibroblast-based bone regeneration by harnessing nanosecond pulsed electric field","authors":"Tian Tu , Chenguang Ouyang , Pengfei Li , Zhipeng Ni , Zewei Wang , Jingtian Lai , Xinhua Chen , Zhenfeng Liu , Hui Lu","doi":"10.1016/j.bioelechem.2025.109089","DOIUrl":"10.1016/j.bioelechem.2025.109089","url":null,"abstract":"<div><div>Despite their terminally differentiated state, human dermal fibroblasts (HDFs) can undergo osteogenic differentiation <em>in vivo</em> under certain pathological conditions, making them promising candidates for bone tissue engineering—though replicating this <em>in vitro</em> would be difficult. Building on prior findings that low-intensity (5 kV/cm) nanosecond pulsed electric fields (nsPEF) can partially reprogram HDFs toward pluripotency and boost their osteogenic capacity, an <em>in vivo</em> bone regeneration complex was fabricated by encapsulating nsPEF-treated cells in a self-healing hydrogel composed of oxidized hyaluronic acid and hydroxypropyl chitosan. In nude mice, these HDFs produced more robust ectopic bone both subcutaneously and within cranial defects, with significantly higher histological scores than untreated controls. RNA sequencing linked this enhanced osteogenesis to activation of the p38 MAPK/YAP pathway and accelerated endochondral ossification, findings that were validated by Western blot, qPCR, and immunofluorescence. The results in this study confirm that nsPEF stimulation markedly improves HDF osteogenic transformation <em>in vitro</em> and <em>in vivo</em>, highlighting its potential as a tool for advancing HDF-based bone regeneration strategies.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"Article 109089"},"PeriodicalIF":4.5,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing the resistance of pipe joint to riboflavin mediated microbial corrosion via increasing heat input 通过增加热输入来增强管道接头对核黄素介导的微生物腐蚀的抵抗力

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-08-21 DOI: 10.1016/j.bioelechem.2025.109087

Lin Liu , Yingying Li , Qin Wang , Rui Liu , Boxin Wei , Tangqing Wu

{"title":"Enhancing the resistance of pipe joint to riboflavin mediated microbial corrosion via increasing heat input","authors":"Lin Liu , Yingying Li , Qin Wang , Rui Liu , Boxin Wei , Tangqing Wu","doi":"10.1016/j.bioelechem.2025.109087","DOIUrl":"10.1016/j.bioelechem.2025.109087","url":null,"abstract":"<div><div>This study investigated the dual role of heat input (HI) and <em>riboflavin</em> on microbiologically influenced corrosion (MIC) of pipe joint. The results showed that localized corrosion of the base metal (BM) and weld zone (WZ) was more severe than that in the heat affected zone (HAZ), but the general corrosion of the former two regions was less severe than that in the latter. The pit depths of BM (8.84–14.61 μm) and WZ (7.71–9.75 μm) were consistently significantly higher than that in HAZ (4.04–5.67 μm). <em>Riboflavin</em> accelerated corrosion via extracellular electron transfer (EET), increasing corrosion rate of BM by 46.8% (from 29.2 μm/y to 42.9 μm/y). The <em>R</em><sup>−1</sup> values (3.59 × 10<sup>−3</sup>–1.01 × 10<sup>−2</sup> Ω<sup>−1</sup>·cm<sup>−2</sup>) were generally higher than those of specimens in medium without <em>riboflavin</em> (2.67 × 10<sup>−3</sup>–8.19 × 10<sup>−3</sup> Ω<sup>−1</sup>·cm<sup>−2</sup>). However, higher HI effectively mitigated this acceleration by coarsening HAZ grains and reducing Widmanstätten structures. HI is a key parameter to optimize the welding process to improve MIC resistance, providing a new strategy for pipeline protection.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"Article 109087"},"PeriodicalIF":4.5,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrochemical RNA aptasensor based on sheet-like α-Fe2O3/Fe3O4 magnetic nanocomposites and CRISPR/Cas13a system for supersensitive detection of osteopontin 基于片状α-Fe2O3/Fe3O4磁性纳米复合材料和CRISPR/Cas13a系统的电化学RNA适体传感器用于骨桥蛋白的超灵敏检测

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-08-20 DOI: 10.1016/j.bioelechem.2025.109085

Yuanyuan Zhou , Zhixiang Lv , Yiyun Geng , Ruijiang Liu

{"title":"An electrochemical RNA aptasensor based on sheet-like α-Fe2O3/Fe3O4 magnetic nanocomposites and CRISPR/Cas13a system for supersensitive detection of osteopontin","authors":"Yuanyuan Zhou , Zhixiang Lv , Yiyun Geng , Ruijiang Liu","doi":"10.1016/j.bioelechem.2025.109085","DOIUrl":"10.1016/j.bioelechem.2025.109085","url":null,"abstract":"<div><div>Osteopontin (OPN) exhibits markedly elevated expression in malignant tumor tissues, rendering it a crucial tumor marker for cancer prevention and monitoring—underscoring the significance of its detection. This work proposed an electrochemical RNA aptasensor based on a novel sheet-like α-Fe<sub>2</sub>O<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub> magnetic nanocomposites (MNCs) and CRISPR/Cas13a system to effectively detect OPN. The proposed aptasensor used the sheet-like α-Fe<sub>2</sub>O<sub>3</sub>/Fe<sub>3</sub>O<sub>4</sub> MNCs as the conduction matrix and applied their magnetic property to accomplish self-assembly of the sensing element onto the electrode. The current change of this aptasensor depended on the activation degree of the CRISPR/Cas13a system, which correlated with the amount of OPN expression. Double-stranded RNA (Apt/Activator) was compelled disassembly due to the OPN's strong affinity of Apt, while single-stranded RNA (Activator) could be guided by crRNA to combine with Cas13a/crRNA and activated the enzymatic activity of Cas13a. Cas13a enzyme affected the current and electrical resistance by shearing the nucleic acid strands (Reporter) on the electrode, ultimately enabling the quantitative detection of OPN. The aptasensor demonstrated excellent selectivity, reproducibility, and stability, with the detection limit (LOD) of 0.33 pg·mL<sup>−1</sup> and the wide linear detection range of 1 pg·mL<sup>−1</sup> − 10 ng·mL<sup>−1</sup>. These results offer a novel idea for advancing tumor marker electrochemical biosensors.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"Article 109085"},"PeriodicalIF":4.5,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-faradaic electrochemical biosensor based on APTES-modified core–shell silica nanoparticles 基于aptes修饰核壳二氧化硅纳米颗粒的非法拉第电化学生物传感器

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-08-19 DOI: 10.1016/j.bioelechem.2025.109086

Munira S. Albuaimi , Ahmed Mohamed El-Toni , Mahmoud Al-Gawati , Abeer Alshammari , Abdullah N. Alodhayb , Khalid E. Alzahrani , Hamad Albrithen , Abdulaziz K. Assaifan

{"title":"Non-faradaic electrochemical biosensor based on APTES-modified core–shell silica nanoparticles","authors":"Munira S. Albuaimi , Ahmed Mohamed El-Toni , Mahmoud Al-Gawati , Abeer Alshammari , Abdullah N. Alodhayb , Khalid E. Alzahrani , Hamad Albrithen , Abdulaziz K. Assaifan","doi":"10.1016/j.bioelechem.2025.109086","DOIUrl":"10.1016/j.bioelechem.2025.109086","url":null,"abstract":"<div><div>Here, SiO<sub>2</sub> nanoparticles-based biosensors were developed for the non-faradaic EIS detection of human cytomegalovirus which is responsible for newborn babies' disability. Mesoporous SiO<sub>2</sub> nanoparticles were fabricated through sol-gel approach using anionic surfactant with an average size of 144 ± 18.6 nm. For biosensing, SiO<sub>2</sub> nanoparticles were drop casted on top of interdigitated gold electrodes on plastic substrate and functionalized with different concentrations of APTES (1, 2, 4 and 8 %). Then, UL83-antibodies were immobilized at the surface via glutaraldehyde. As APTES concentration increased, the sensitivity decreased from 13.99 to 10.78 nF/ln(ng/mL). However, the limit of detection improved from 6 to 2 ng/mL. Before biosensing, the coverage of APTES at the surface was characterized with XPS and faradaic EIS. Furthermore, the effect of APTES functionalization approach on the coverage of SiO<sub>2</sub> nanoparticles at the surface was investigated. APTES was introduced before and after drop casting SiO<sub>2</sub> nanoparticles. APTES functionalization after drop casting yielded better SiO<sub>2</sub> nanoparticles coverage on the surface which is essential for non-faradaic EIS biosensing. Uneven coverage would result in deteriorated biosensing performances, especially for non-faradaic biosensors which rely on interfacial capacitive changes upon bioreceptor/antigen interactions.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"Article 109086"},"PeriodicalIF":4.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile synthesis of highly active PtSnCoNi hierarchical dendritic nanowires greatly boosting signal amplification for ultrasensitive immunoassay of biomarker HER-2 高活性PtSnCoNi分层树突状纳米线的简单合成极大地增强了生物标志物HER-2的超灵敏免疫分析信号放大

IF 4.5 2区化学

Bioelectrochemistry Pub Date : 2025-08-17 DOI: 10.1016/j.bioelechem.2025.109084

Yan-Yu Shi , Jin-Yu Li , Xiao-Yan Chen , Ai-Jun Wang , Jiu-Ju Feng , Tuck Yun Cheang

{"title":"Facile synthesis of highly active PtSnCoNi hierarchical dendritic nanowires greatly boosting signal amplification for ultrasensitive immunoassay of biomarker HER-2","authors":"Yan-Yu Shi , Jin-Yu Li , Xiao-Yan Chen , Ai-Jun Wang , Jiu-Ju Feng , Tuck Yun Cheang","doi":"10.1016/j.bioelechem.2025.109084","DOIUrl":"10.1016/j.bioelechem.2025.109084","url":null,"abstract":"<div><div>Human epidermal growth factor receptor-2 (HER-2), a key biomarker in breast cancer, is critical for early diagnosis and prognosis evaluation. In this work, a label-free electrochemical immunosensor was developed for biomarker HER-2 detection based on PtSnCoNi hierarchical dendritic alloyed nanowires (PtSnCoNi HDNWs). These nanowires were synthesized via a co-reduction-triggered anisotropic growth strategy in oleylamine. Their dendritic structure, multiple compositions, and superior catalytic property for H<sub>2</sub>O<sub>2</sub> reduction were rigorously characterized by multiple technologies. This biosensor demonstrated a broad linear range from 0.1 to 1,000,000 pg mL<sup>−1</sup> (<em>R</em><sup>2</sup> = 0.9985) and a low detection limit of 0.068 pg mL<sup>−1</sup> (S/N = 3). It performed well in human serum samples, achieving recovery rates ranging from 103.8 % to 105.3 % and a relative standard deviation below 3.7 %, validating its clinical applicability. This work highlights the potential of advanced alloy materials in developing high-performance biosensors for early cancer biomarker detection.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109084"},"PeriodicalIF":4.5,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0