Bioelectrochemistry最新文献_第4页

Print-Light-Synthesis of ruthenium oxide thin film electrodes for electrochemical sensing applications

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-15 DOI: 10.1016/j.bioelechem.2025.108909

Stefano Gianvittorio , Marco Malferrari , Horst Pick , Stefania Rapino , Andreas Lesch

{"title":"Print-Light-Synthesis of ruthenium oxide thin film electrodes for electrochemical sensing applications","authors":"Stefano Gianvittorio , Marco Malferrari , Horst Pick , Stefania Rapino , Andreas Lesch","doi":"10.1016/j.bioelechem.2025.108909","DOIUrl":"10.1016/j.bioelechem.2025.108909","url":null,"abstract":"<div><div>Print-Light-Synthesis (PLS) combines the inkjet printing of a ruthenium precursor ink with the simultaneous photo-induced generation of ruthenium oxide films. During PLS, inkjet-printing generates on conductive as well as insulating substrates micrometer-thin reaction volumes that contain with high precision defined precursor loadings. Upon direct UV light irradiation, the Ru precursor converts to RuO<sub>2</sub> while all other ink components escape in the gas phase. No post PLS processes are required, and the as-obtained RuO<sub>2</sub> films can be immediately used as electrochemical devices. Two-dimensional RuO<sub>2</sub> patterns with micrometric resolution and highly-controlled ruthenium loadings (few µg/cm<sup>2</sup>) are realized. Thin RuO<sub>2</sub> films are generated on insulating substrates, such as polyimide, as well as individual RuO<sub>2</sub> particles on conductive substrates, such as graphene layers. The RuO<sub>2</sub> films are characterized by electron microscopy and spectroscopic techniques. The sensoristic applicability of the PLS-RuO<sub>2</sub> electrodes is demonstrated by potentiometric pH sensing in cell cultures and amperometric detection of L-cysteine. For pH sensing the RuO<sub>2</sub> film electrodes show Nernstian sensitivity. L-cysteine detection of RuO<sub>2</sub>-modified graphene electrodes showed an electrocatalytical effect and resulted in the possibility of selectively detecting L-Cysteine also in presence of the interfering compound uric acid.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108909"},"PeriodicalIF":4.8,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced detection of Cystatin C for predicting adverse outcomes in gestational diabetes mellitus using a point-of-care immunosensor 利用即时免疫传感器增强胱抑素C检测，预测妊娠期糖尿病的不良结局。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-13 DOI: 10.1016/j.bioelechem.2025.108907

Ya Jin , Yongmei Chen , Xiaoqin Li , Songjun Han , Lei Mou , Nan Li

{"title":"Enhanced detection of Cystatin C for predicting adverse outcomes in gestational diabetes mellitus using a point-of-care immunosensor","authors":"Ya Jin , Yongmei Chen , Xiaoqin Li , Songjun Han , Lei Mou , Nan Li","doi":"10.1016/j.bioelechem.2025.108907","DOIUrl":"10.1016/j.bioelechem.2025.108907","url":null,"abstract":"<div><div>Cystatin C (Cys-C) is emerging as a critical biomarker for assess gestational diabetes mellitus (GDM), a condition that significantly impacts maternal and fetal health. In this study, we developed a novel label-free electrochemical immunosensor designed for point-of-care applications, offering lower reagent consumption and rapid detection of Cys-C in pregnant women with GDM. Compared to traditional enzyme-linked immunosorbent assays (ELISA), the sensor demonstrates enhanced sensitivity, reduced reagent usage, and faster detection. In a cohort study involving 150 pregnant women with GDM and 150 healthy controls, serum Cys-C levels were analyzed using the developed sensor. Serum samples were collected and analyzed for Cys-C levels using our immunosensor. Serum Cys-C levels were significantly elevated in GDM patients compared to controls (P < 0.05), and higher levels were observed in GDM complicated by pregnancy-induced hypertension (PIH) and fetal growth restriction (FGR). Furthermore, elevated Cys-C levels were positively correlated with adverse pregnancy outcomes, including premature birth, fetal distress, and cesarean section (P < 0.05). This study underscores the potential of Cys-C as a reliable biomarker for GDM and highlights the advantages of our novel sensor for quick, accurate, and scalable GDM diagnosis and management.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108907"},"PeriodicalIF":4.8,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conductive and flexible gold-coated polylactic acid nanofiber-based electrochemical aptasensor for monitoring cortisol level in sweat and saliva 用于监测汗液和唾液中皮质醇水平的导电柔性金包覆聚乳酸纳米纤维电化学适体传感器。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-13 DOI: 10.1016/j.bioelechem.2025.108908

Mkliwa Koumbia, Takai Madoka

{"title":"Conductive and flexible gold-coated polylactic acid nanofiber-based electrochemical aptasensor for monitoring cortisol level in sweat and saliva","authors":"Mkliwa Koumbia, Takai Madoka","doi":"10.1016/j.bioelechem.2025.108908","DOIUrl":"10.1016/j.bioelechem.2025.108908","url":null,"abstract":"<div><div>Conductive nanofibers can exhibit excellent mechanical properties such as flexibility, elasticity, porosity, large surface area-to-volume ratio, etc making them suitable for a wide range of applications including biosensor development. Their large surface area provides more active sites for immobilization of large amount of bioreceptors enabling more interaction sites with the target analytes, enhancing sensitivity and detection capabilities. However, engineering conductive nanofibers with such excellent properties is challenging limiting their effective deployment for intended applications. In this research, we propose a novel approach for easy fabrication of highly conductive and flexible nanofiber leveraging the electrospinning, electroless deposition and have applied it to cortisol monitoring; a common biomarker for stress which is often quantified through enzyme-linked immunoassays using blood or saliva samples. By adopting the nanofiber sheet as a transducer for aptamer immobilization and cortisol sensing our developed biosensor was able to detect cortisol in buffer, artificial saliva, and artificial sweat within five minutes, from 10 pg/mL to 10 µg/mL (27.59 pM to 27.59 µM) with a low detection limit of 1 pg/ml (2.76 pM). The Au-coated PLA nanofiber-based electrochemical biosensor’s flexibility allows for compact manufacturing, rendering it an optimal choice for integration into point-of-care testing and wearable systems.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108908"},"PeriodicalIF":4.8,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized detection of calcium ion in serum using constant potential coulometry with metastable liquid–liquid contact doping enhanced PEDOT: PSS ink 亚稳液-液接触掺杂增强PEDOT: PSS墨水恒电位库仑法优化血清钙离子检测。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-10 DOI: 10.1016/j.bioelechem.2025.108903

Suyun Wei , Jie Zhao , Shaojun Ke , Mingjia Zou , Qingze Han , Guofeng Cui

{"title":"Optimized detection of calcium ion in serum using constant potential coulometry with metastable liquid–liquid contact doping enhanced PEDOT: PSS ink","authors":"Suyun Wei , Jie Zhao , Shaojun Ke , Mingjia Zou , Qingze Han , Guofeng Cui","doi":"10.1016/j.bioelechem.2025.108903","DOIUrl":"10.1016/j.bioelechem.2025.108903","url":null,"abstract":"<div><div>Highly stable calcium ion selective electrodes (Ca<sup>2+</sup>-ISEs) were developed by drop-casting a layer of poly(3,4-ethylenedioxythiophene): polystyrene sulfonate (PEDOT: PSS) as an ion-to-electron transfer layer onto Au electrode. The conductive PEDOT: PSS ink was prepared using a metastable liquid–liquid contact (MLLC) doping method, which induced phase separation, removed excess PSS, and significantly enhanced charge transfer kinetics and conductivity. The resulting Ca<sup>2+</sup>-ISEs exhibited excellent electrochemical performance. Potentiometric studies revealed a significant sensitivity of 33.1 ± 0.98 mV/decade (N = 3) with a high potential stability of 3.16 ± 2.53 μV/h. Importantly, Ca<sup>2+</sup>-ISEs combined with the constant potential coulometry method, the lower detection limit was optimized to 8.527 × 10<sup>−8</sup> M (LOD = 3σ/s, N = 3). The performance of the Ca<sup>2+</sup>-ISE system was evaluated in inactivated fetal bovine serum using constant potential coulometry, demonstrating the highest measurement accuracy compared to potentiometric and chronoamperometric. The enhanced PEDOT: PSS-MLLC based Ca<sup>2+</sup>-ISEs combined with the constant potential coulometry method developed in this research demonstrate considerable potential for clinical applications in blood ion analysis.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108903"},"PeriodicalIF":4.8,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile preparation of bifunctional monolayers through diazonium grafting and “click” postfunctionalization: A first step towards efficient aptasensing interfaces 通过重氮接枝和“点击”后功能化轻松制备双功能单层：迈向高效适配界面的第一步。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-10 DOI: 10.1016/j.bioelechem.2025.108904

Andra Mihaela Onaş , Andreea Mădălina Pandele , Anamaria Hanganu , Ciprian Victor Florea , Horia Iovu , Matei D. Raicopol , Luisa Pilan

{"title":"Facile preparation of bifunctional monolayers through diazonium grafting and “click” postfunctionalization: A first step towards efficient aptasensing interfaces","authors":"Andra Mihaela Onaş , Andreea Mădălina Pandele , Anamaria Hanganu , Ciprian Victor Florea , Horia Iovu , Matei D. Raicopol , Luisa Pilan","doi":"10.1016/j.bioelechem.2025.108904","DOIUrl":"10.1016/j.bioelechem.2025.108904","url":null,"abstract":"<div><div>Herein, we present an efficient approach for developing electrochemical aptasensing interfaces, by “click” postfunctionalization of phenylethynyl-grafted glassy carbon substrates with mixed monolayers containing biorecognition elements and phosphorylcholine zwitterionic groups. Typically, controlling the composition of multicomponent surface layers by grafting from a mixture of aryldiazonium salts is challenging due to differences in their chemical reactivity. Our approach circumvents this issue by employing the electrochemical reduction of a single aryldiazonium salt containing a silyl-protected alkyne group followed by deprotection, to create phenylethynyl monolayers which can subsequently accommodate the concurrent immobilization of bioreceptors and zwitterionic groups through “click” postfunctionalization. We show that the surface ratio of the components in the bifunctional monolayers, estimated through XPS and electrochemical methods, can be accurately controlled by adjusting the mole ratio of the corresponding azide reagents in the “click” coupling solution. Moreover, electrochemical impedance spectroscopy and fluorescence microscopy investigations on bifunctional monolayers containing ssDNA and phosphorylcholine groups reveal that they effectively prevent nonspecific protein adsorption, while maintaining sufficiently low impedance to facilitate electrochemical detection. Finally, we demonstrate that proof of concept aptasensing interfaces based on binary layers containing a ferrocene-tagged cocaine/quinine aptamer and phosphorylcholine groups exhibit a trade-off between an improved analytical response and antifouling efficiency.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108904"},"PeriodicalIF":4.8,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid and receptor-free Prussian blue electrochemical sensor for the detection of pathogenic bacteria in blood 快速无受体普鲁士蓝电化学传感器检测血液中致病菌。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-09 DOI: 10.1016/j.bioelechem.2025.108902

Sriramprabha Ramasamy, Sekar Madhu, Jungil Choi

{"title":"Rapid and receptor-free Prussian blue electrochemical sensor for the detection of pathogenic bacteria in blood","authors":"Sriramprabha Ramasamy, Sekar Madhu, Jungil Choi","doi":"10.1016/j.bioelechem.2025.108902","DOIUrl":"10.1016/j.bioelechem.2025.108902","url":null,"abstract":"<div><div>Bloodstream bacterial infections, a major health concern due to rising sepsis rates, require prompt, cost-effective diagnostics. Conventional methods, like CO<sub>2</sub>-based transduction, face challenges such as volatile metabolites, delayed gas-phase signaling, and the need for additional instruments, whereas electrochemical sensors provide rapid, sensitive, and efficient real-time detection. In this study, we developed a bioreceptor-free Prussian blue (PB) sensor platform for real-time bacterial growth monitoring in blood culture. PB thin films were electrodeposited onto a screen-printed carbon electrode (SPCE) via cyclic voltammetry (CV) technique under optimal conditions. The electrochemical performance of PB/SPCE was assessed using differential pulse voltammetry (DPV) against exoelectrogenic bacteria, including <em>E. coli</em>, <em>P. aeruginosa</em>, <em>S. aureus,</em> and <em>E. faecalis</em>. The proposed sensor exhibited surface-controlled electrochemical kinetics and bacteria-driven metal reduction from PB to Prussian white (PW), facilitated by extracellular electron transfer (EET). It showed significant sensitivity with an extensive detection range of 10<sup>2</sup>–10<sup>8</sup> CFU/mL for <em>E. coli</em> and <em>S. aureus</em>, and 10<sup>3</sup>–10<sup>8</sup> CFU/mL for <em>P. aeruginosa</em> and <em>E. faecalis</em>, with reliable detection limits. The sensor accessed the viability of the pathogen within 3 hrs, offering a rapid, efficient alternative to traditional, labor-intensive methods for blood-based diagnostics.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108902"},"PeriodicalIF":4.8,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A carbon fiber modified with tin oxide/graphitic carbon nitride as an electrochemical indirect competitive immuno-sensor for ultrasensitive aflatoxin M1 detection 用氧化锡/氮化石墨碳修饰的碳纤维作为超灵敏黄曲霉毒素 M1 检测的电化学间接竞争免疫传感器。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-09 DOI: 10.1016/j.bioelechem.2025.108898

Iram Naz , Muhammad Nasir , Mian Hasnain Nawaz , Silvana Andreescu , Akhtar Hayat , Farhat Jubeen

{"title":"A carbon fiber modified with tin oxide/graphitic carbon nitride as an electrochemical indirect competitive immuno-sensor for ultrasensitive aflatoxin M1 detection","authors":"Iram Naz , Muhammad Nasir , Mian Hasnain Nawaz , Silvana Andreescu , Akhtar Hayat , Farhat Jubeen","doi":"10.1016/j.bioelechem.2025.108898","DOIUrl":"10.1016/j.bioelechem.2025.108898","url":null,"abstract":"<div><div>The importance of developing multifunctional nanomaterials for sensing technologies is increasing with the arrival of nanotechnology. In this study, we describe the introduction of novel nanoprobe electro-active material into the architecture of an electrochemical immuno-sensor. Based on the electrochemical immuno-sensor, functionalized tin oxide/graphitic carbon nitride nanocomposite (fSnO<sub>2</sub>/g-C<sub>3</sub>N<sub>4</sub>) was synthesized and then analyte specific anti-aflatoxin M<sub>1</sub> monoclonal antibody (AFM<sub>1</sub>-ab) combined to form an electro-active nanoprobe (fSnO<sub>2</sub>/g-C<sub>3</sub>N<sub>4</sub>/AFM<sub>1</sub>-ab). First, aflatoxin M<sub>1</sub> (AFM<sub>1</sub>) conjugated bovine serum albumin (BSA-AFM<sub>1</sub>) was electro-oxidized on the surface of carbon fiber (CF) followed by the consequent addition of nanoprobe. The formation of nanocomposite was substantiated through various characterization techniques, Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Dynamic light scattering (DLS). Immuno-sensor fabrication was characterized via Field emission scanning electron microscopy (FE-SEM), optical microscope images, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). This immuno-sensor demonstrated good reproducibility, selectivity, specificity and sensitivity for AFM<sub>1</sub> (LOD of 0.03 ng mL<sup>−1</sup>). Following spiking, this immuno-sensor produced good recovery values in the range of 94–96 % against real sample, such as milk. The development of sophisticated sensing methods for a range of analytes can greatly benefit from the widespread application of this innovative immuno-sensing approach.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108898"},"PeriodicalIF":4.8,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TdT combined with Cas14a for the electrochemical biosensing of NPC-derived exosomes TdT 与 Cas14a 结合用于鼻咽癌外泌体的电化学生物传感。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-06 DOI: 10.1016/j.bioelechem.2025.108900

Zhong Gao , Jingjian Liu , Yu Zhang , Ronghua Xu , Yang Yang , Lun Wu , Jinan Lei , Tingwen Ming , Fangling Ren , Li Liu , Qinhua Chen

{"title":"TdT combined with Cas14a for the electrochemical biosensing of NPC-derived exosomes","authors":"Zhong Gao , Jingjian Liu , Yu Zhang , Ronghua Xu , Yang Yang , Lun Wu , Jinan Lei , Tingwen Ming , Fangling Ren , Li Liu , Qinhua Chen","doi":"10.1016/j.bioelechem.2025.108900","DOIUrl":"10.1016/j.bioelechem.2025.108900","url":null,"abstract":"<div><div>In this work, the electrochemical biosensor based on the subtle combination of terminal deoxynucleotidyl transferase (TdT), CRISPR/Cas14a, and magnetic nanoparticles (MNPs) was developed for the detection of nasopharyngeal carcinoma (NPC)-derived exosomes. Due to the synergistic effect of the following factors: the powerful elongation capacity of TdT for single-stranded DNA (ssDNA) with 3-hydroxy terminus, the outstanding <em>trans</em>-cleavage ability of CRISPR/Cas14a specifcally activated by the crRNA binding to target DNA, and the excellent separation ability of MNPs, the developed electrochemical biosensor exhibited high sensitivity for the detection of NPC-derived exosome, with a linear range from 6.0 × 10<sup>2</sup> ∼ 1.0 × 10<sup>5</sup> particles/mL and a limit of detection as lown as 80 particles/mL. In addition, this electrochemical biosensor successfully distinguished exosomes from NPC patients and healthy individuals. This electrochemical biosensor opens up a new pathway for the early diagnosis of NPC.</div><div>Abbreviations: NPC, Nasopharyngeal carcinoma; CRISPR/Cas, Clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins system; PAMs, Protospacer adjacent motifs; RCA, Rolling circle amplification; CHA, Catalytic hairpin assembly; LAMP, Loop-mediated isothermal amplification; TdT, Terminal deoxynucleotidyl transferase; SgRNA, Single guide RNA.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108900"},"PeriodicalIF":4.8,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a sandwich-type electrochemical DNA sensor based on CeO2/AuPt nanoprobes for highly sensitive detection of hepatitis B virus DNA 基于CeO2/AuPt纳米探针的三明治型电化学DNA传感器的研制，用于乙型肝炎病毒DNA的高灵敏度检测。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-05 DOI: 10.1016/j.bioelechem.2025.108901

Jian Mao, Jiaxin Wang, Hongli Chen, Qinghua Yan

{"title":"Development of a sandwich-type electrochemical DNA sensor based on CeO2/AuPt nanoprobes for highly sensitive detection of hepatitis B virus DNA","authors":"Jian Mao, Jiaxin Wang, Hongli Chen, Qinghua Yan","doi":"10.1016/j.bioelechem.2025.108901","DOIUrl":"10.1016/j.bioelechem.2025.108901","url":null,"abstract":"<div><div>To provide accurate diagnostic evidence for early hepatitis B virus (HBV) infection-related diseases, this study targeted HBV DNA as an analyte, where a sandwich-type electrochemical DNA sensor based on gold nanoparticles/reduced graphene oxide (Au NPs/ERGO) and cerium oxide/gold–platinum nanoparticles (CeO<sub>2</sub>/AuPt NPs) was constructed. Au NPs/ERGO composite nanomaterials were first synthesized on the surface of a glass carbon electrode using electrochemical co-reduction, which significantly improved the specific surface area and electrical conductivity of the electrode. Further specific hybridization of target HBV-DNA was performed by combining capture probe DNA (S1-DNA) bound to AuNPs/ERGO with CeO<sub>2</sub>/AuPt modified signal probe DNA (S2-DNA). Leveraging the excellent H<sub>2</sub>O<sub>2</sub> catalytic activity of the CeO<sub>2</sub>/AuPt nanocomposite, the constructed sandwich-type electrochemical DNA sensor was used to detect HBV DNA. By optimizing the detection conditions, the sensor showed a good linear response in the range of 1 fmol/L to 1 nmol/L, with a detection limit as low as 0.36 fmol/L. The sensor had good specificity, repeatability, and stability. Further, spiked recovery experiments of actual serum samples showed recoveries ranging from 98.7 % to 102.7 %, and the relative standard deviations were all lower than 4.77 %. This study provides a new method for the detection of HBV DNA with potential clinical applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108901"},"PeriodicalIF":4.8,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Ni-ZnO-ACE-2 peptide hybrids as electrochemical devices for SARS-CoV-2 spike protein detection Ni-ZnO-ACE-2多肽杂合体电化学检测SARS-CoV-2刺突蛋白的研制

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2025-01-04 DOI: 10.1016/j.bioelechem.2025.108899

Freddy.A. Nunez , Marcos R. de A. Silva , Eduardo M. Cilli , Sarah T.R. Brandão , Martin Müller , Dieter Fischer , Quinn A. Besford , Wendel A. Alves

{"title":"Development of Ni-ZnO-ACE-2 peptide hybrids as electrochemical devices for SARS-CoV-2 spike protein detection","authors":"Freddy.A. Nunez , Marcos R. de A. Silva , Eduardo M. Cilli , Sarah T.R. Brandão , Martin Müller , Dieter Fischer , Quinn A. Besford , Wendel A. Alves","doi":"10.1016/j.bioelechem.2025.108899","DOIUrl":"10.1016/j.bioelechem.2025.108899","url":null,"abstract":"<div><div>Owing to fast SARS-CoV-2 mutations, biosensors employing antibodies as biorecognition elements have presented problems with sensitivity and accuracy. To face these challenges, antibodies can be replaced with the human angiotensin converting enzyme 2 (ACE-2), where it has been shown that the affinity between ACE-2 and the receptor binding domain (RBD) increases with the emergence of new variants. Herein, we report on Ni-doped ZnO nanorod electrochemical biosensors employing an ACE-2 peptide (IEEQAKTFLDKFNHEAEDLFYQS-NH<sub>2</sub>) as a biorecognition element for detecting Spike (S) Wild-Type (WT) protein. The electrode was fully characterized in terms of electrochemical and physical properties. The sensor showed high cross reactivity with Spike protein B.1.1.7 and Spike protein B.1.351. Still, there was no cross reactivity with the Nucleocapsid protein WT, showing that the biosensor can identify ancestral WT S protein and S protein variants of concern. The device exhibited a LOD of 60.13 ng mL<sup>−1</sup> across an S protein WT concentration range from 200 ng mL<sup>−1</sup> to 1000 ng mL<sup>−1</sup> and a LOQ of 182.22 ng mL<sup>−1</sup>. The calculated sensitivity and specificity were 88.88 and 100 %, respectively. These results proved that the Ni-ZnO sensor has promising prospects for SARS-CoV-2 detection and diagnosis of other viruses, employing peptides as biorecognition elements.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"Article 108899"},"PeriodicalIF":4.8,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0