Bioelectrochemistry最新文献

{"title":"Effect of electroporation on neuronal excitability under H-FIRE pulses","authors":"Fei Guo,&nbsp;Li Luo,&nbsp;Chunhuai Gong,&nbsp;Kai Pei","doi":"10.1016/j.bioelechem.2025.109109","DOIUrl":"10.1016/j.bioelechem.2025.109109","url":null,"abstract":"<div><div>The traditional Hodgkin-Huxley (HH) model is mainly applicable to neuronal excitability under low electric fields but ails at high field intensities. In this study, an improved HH model incorporating electroporation (EP) current was proposed. Simulation results showed that under a classical IRE pulse (100 μs, 440 V/cm), EP current evoked an action potential (AP) with a peak of 18.38 mV at <em>t</em> = 2.98 ms; whereas under a burst of H-FIRE pulses (1–1-1-1 μs, 440 V/cm), the AP peak decreased to 13.05 mV (by 5.33 mV) and was delayed to <em>t</em> = 26.82 ms. Further analysis revealed that both IRE and H-FIRE stimulation have an optimal electric field window: moderate increases in field strength enhanced excitability, while excessive intensity caused inhibitory effects due to over-electroporation. In addition, prolonging the inter-phase delay, inter-pulse delay, and pulse width of H-FIRE pulses aggravated EP effects and significantly suppressed excitability. Compared with the classical HH model, the proposed model more accurately reflects neuronal excitability under high electric fields and has important implications for the study of single-neuron stimulation.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109109"},"PeriodicalIF":4.5,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An electrochemical biosensor for immobilization coupling and quantifying exosomes based on a UiO-66 metal−organic framework","authors":"Zhangmin Wang ,&nbsp;Xiaoyu Fan ,&nbsp;Xiaobo Hu ,&nbsp;Xueliang Wang","doi":"10.1016/j.bioelechem.2025.109108","DOIUrl":"10.1016/j.bioelechem.2025.109108","url":null,"abstract":"<div><div>Extracellular vesicles, particularly exosomes, have garnered attention as promising new cancer biomarkers in recent decades. Exosome membrane proteins act as a source of information about their parent cells and offer readily accessible targets. Here, we presented a sensitive electrochemical biosensor based on a Zr-based UiO-66 metal-organic framework for immobilization coupling, and quantifying lung-cancer-derived exosomes. In this design, the carcinoembryonic antigen (CEA) aptamer identified and bound the CEA protein on the exosome surface, and the UiO-66 was immobilized, coupling with the phosphate groups of exosomes. The biosensor demonstrated a higher sensitivity than most previously described approaches, with a wider linear range (10² to 5 × 10⁶ particles/μL) and a lower limit of detection (7 particles/μL). Moreover, the biosensor exhibited clinical potential by selectively binding CEA-specific exosomes across distinct lung cancer subtypes. Our proposed biosensor offers numerous application opportunities for early clinical diagnosis, along with the benefits of affordability, excellent sensitivity, and simple operation.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109108"},"PeriodicalIF":4.5,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ biological ozone detection by measuring electrochemical impedances of plant tissues","authors":"Serge Kernbach","doi":"10.1016/j.bioelechem.2025.109088","DOIUrl":"10.1016/j.bioelechem.2025.109088","url":null,"abstract":"<div><div>This work demonstrates the biological detection of low-level <figure><img></figure> by measuring electrochemical impedances of stem tissues in tobacco and tomato plants, both indoor and outdoor. Ozone concentrations as low as 30 <figure><img></figure> above ambient levels were detected via physiological responses, enabling the use of phytosensors as biodetectors of environmental pollutants. <figure><img></figure> exposure affects stomatal regulation that in turn alters the hydrodynamics of fluid transport system in plants. The measurement results indicate a reaction of hydrodynamic system to changes in <figure><img></figure> concentration with a delay of 10–20 min between the onset of exposure and biological response. The probability of false-negative responses from a plant is 0.15 ± 0.06. Pooling data from at least three plants allows for 92% confidence in detecting excess <figure><img></figure> . Measurements on days with low and high ozone levels of 80 <figure><img></figure> to 130 <figure><img></figure> result in a 2.33-fold difference in sensor readings at these levels, underscoring the sensitivity of the method. Statistical robustness is supported by 948 plant-sensor measurements with 9 plants over 51 days, totaling 10<span><math><msup><mrow></mrow><mrow><mn>7</mn></mrow></msup></math></span> samples via automated monitoring. Long-term field tests demonstrate the reliability of electrochemical methods. This approach has applications in environmental monitoring, biological pollution detection and biosensing.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109088"},"PeriodicalIF":4.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocorrosion studies on borated and non-borated 304 L stainless steel using Bacillus subtilis SNF-1, a bacterial isolate from SNF pool","authors":"Namrata Upadhyay ,&nbsp;Sudhir K. Shukla ,&nbsp;N. Malathy ,&nbsp;Y.V. Nancharaiah ,&nbsp;A. Ravi Shankar ,&nbsp;S. Ningshen","doi":"10.1016/j.bioelechem.2025.109106","DOIUrl":"10.1016/j.bioelechem.2025.109106","url":null,"abstract":"<div><div>This study investigates microbiologically-influenced corrosion (MIC) aspects of borated and non-borated 304 L- stainless-steel using <em>Bacillus subtilis</em> SNF-1, which was isolated from the spent nuclear fuel pool (SNF). Over 28 days, electrochemical analyses revealed distinct corrosion behaviours: borated 304 L SS exhibited a more pronounced decrease in open circuit potential (from 0.03 to −0.35 V vs. Ag/AgCl) as compared to non-borated 304 L SS (from 0.05 to −0.10 V vs. Ag/AgCl) indicating higher susceptibility to MIC. Potentiodynamic polarization studies revealed an increase in passive current density (from 1.5 to 2.4 μA.cm⁻² for non-borated 304 L SS and from 2.4 to 3.4 μA.cm⁻² for borated 304 L SS), along with a lower pitting potential indicating the role of <em>B. subtilis</em> SNF-1 in MIC. Electrochemical impedance spectroscopy confirmed accelerated degradation, with polarization resistance (R_p) dropping by 69 % in borated 304 L SS and 86 % in non-borated 304 L SS. Despite higher absolute corrosion rates in borated 304 L SS, non-borated 304 L SS experienced a greater relative increase in corrosion (3.8-fold vs. 2.3-fold) due to denser biofilm coverage (95 % vs. 74 %). Surface analysis identified localized pitting beneath biofilms, exacerbated by boride-induced micro-galvanic effects. These findings underscore the dual role of alloy microstructure and biofilm dynamics in MIC severity.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109106"},"PeriodicalIF":4.5,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-electrochemical nitrogen removal in wastewater: Coupling anodic ammonium oxidation with hydrogenotrophic denitrification in a microbial electrolysis cell","authors":"Afrooz Bayat ,&nbsp;Ricardo Bello-Mendoza","doi":"10.1016/j.bioelechem.2025.109107","DOIUrl":"10.1016/j.bioelechem.2025.109107","url":null,"abstract":"<div><div>The increasing need for efficient nitrogen removal in wastewater treatment has driven interest in innovative biological and electrochemical approaches that can simultaneously address ammonium and nitrate contamination. This study explored the performance of simultaneous anodic ammonium oxidation and hydrogenotrophic denitrification in a single-chamber microbial electrolysis cell (MEC). Different operational conditions, including varying ammonia‑nitrogen/nitrate‑nitrogen ratios, were selected to evaluate the MEC's ability to promote anaerobic ammonium oxidation along with organic matter removal. The results demonstrated that the single-stage MEC achieved 57.8 % total nitrogen removal efficiency under an optimal condition where the influent contained only ammonium nitrogen at 200 mg/L with no added nitrate. Adding nitrate in the influent of the microbial electrolysis system decreased ammonium removal in all the operational conditions. Additionally, chemical oxygen demand (COD) destruction and methane production occurred uninterrupted throughout all experimental stages. Therefore, simultaneous methane production and nitrogen removal was realized in this study. The microbial electrolysis system achieved 97.3 ± 0.5 % nitrate removal, outperforming the hydrogenotrophic anaerobic digestion system. While no nitrite accumulation was observed in the systems, nitrous oxide emission occurred in all the operational conditions. A microbial community analysis showed that the anode of the MEC reactor was dominated by Pseudothauera and clostridium, which explained the denitrification performance by the MEC. This highlights the potential of bioelectrochemical systems for simultaneous ammonium and nitrate removal in a single-stage wastewater treatment process.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109107"},"PeriodicalIF":4.5,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ratiometric electrochemical sensor based on metal-organic framework and nanosilver as response signals for cTnI detection","authors":"Yanju Liu ,&nbsp;Gao Si ,&nbsp;Yuning Zhao ,&nbsp;Zhixiang Liu ,&nbsp;Xiaohua He ,&nbsp;Huaixia Yang ,&nbsp;Liying Zhao","doi":"10.1016/j.bioelechem.2025.109099","DOIUrl":"10.1016/j.bioelechem.2025.109099","url":null,"abstract":"<div><div>The concentration of cardiac troponin I (cTnI) serves as a well-established biomarker of myocardial injury. In this work, a ratiometric electrochemical aptasensor utilizing Zirconium-metal organic framework (UiO-66) and AgNPs as electrochemical signaling tags were developed for the ultrasensitive detection of cardiac troponin I (cTnI). Upon specific binding between cTnI and the aptamer, probe 1 (P1) dissociated from the aptamer, resulting in a decreased UiO-66 signal. Meanwhile, signal amplification mediated by terminal deoxynucleotide transferase (TdT) provided a substantial quantity of active sites for surface-initiated reversible addition fragmentation chain transfer (SI-RAFT) polymerization, leading to a massive amount of silver nano-particles deposited on the electrode surfaces with enhanced silver ion signals. The concentration range of cTnI was determined to be 1 × 10⁻³–1 × 10² ng mL⁻¹, with a detection limit as low as 20.53 fg mL⁻¹. Notably, the ratiometric sensor performed well in detecting cTnI in serum samples from patients, indicating clinical application potential. Importantly, the aptasensor exhibited excellent performance in detecting cTnI in human serum samples, highlighting its strong potential for clinical application.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109099"},"PeriodicalIF":4.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal exopolysaccharides as a bio-binder for conductive pigment/reduced graphene oxide/nano‑cobalt composite: an eco-friendly solution for anode rechargeable batteries","authors":"Nashwa.M. Yousif ,&nbsp;Reham M.M. AbdelKader ,&nbsp;Ola.M. Gomaa","doi":"10.1016/j.bioelechem.2025.109094","DOIUrl":"10.1016/j.bioelechem.2025.109094","url":null,"abstract":"<div><div>The rapid increase in population has driven the demand for fossil fuel energy, contributing to increased carbon emissions that ultimately accelerate global warming and climate change. Battery storage systems have many advantages over conventional energy sources. However, they face limitations such as energy storage, cost, and environmental hazards that come with the use of chemical binders. This study presents a novel application of <em>Talaromyces atroroseus</em> co-produced exopolysaccharides (EPS)-pigment complex for their bio-binding and conductivity properties that can replace chemical binders in the preparation of rechargeable anode batteries. The EPS–pigment complex was combined with reduced graphene oxide (rGO) and nano‑cobalt (CoNPs) to fabricate a hybrid conductive composite on nickel foam mesh. The composite was characterized using Energy Dispersive X-ray (EDX) and X-ray diffraction (XRD), both confirmed the presence of C, O, and Co. Electrochemical characterization of the composite elements showed a cathodic peak at 0.2 V and an anodic peak at 0.414 V. At the same time, the specific capacitance reached 400 F/g for the composite. The results also demonstrated a good rate of charge and discharge. Compared to chemical binders, fungal EPS showed good binding strength and environmental benefits due to its bio-based nature.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109094"},"PeriodicalIF":4.5,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of phenylboronic acid modified graphene oxide-silica biosensor for label-free electrochemical detection of circulating tumor cells","authors":"Sabeen Iqbal ,&nbsp;Fahmida Jabeen ,&nbsp;Nabila Bashir ,&nbsp;Syed Tayyab Raza Naqvi ,&nbsp;Saadat Majeed ,&nbsp;Azhar Rasul ,&nbsp;Muhammad Najam-ul-Haq","doi":"10.1016/j.bioelechem.2025.109098","DOIUrl":"10.1016/j.bioelechem.2025.109098","url":null,"abstract":"<div><div>Early and accurate detection of circulating tumor cells (CTCs) is vital for cancer diagnosis and personalized treatment. Despite their clinical significance, the identification of CTCs remains challenging because of the biological complexity and lower concentration. Therefore, a cost-effective, and label-free electrochemical biosensor based on phenyl boronic acid functionalized graphene oxide-silica (PBA@GO-SiO₂) is developed to selectively recognize sialic acid-rich glycoproteins on HepG2 cells. The electrochemical properties of developed biosensor confirm a concentration-dependent response upon interaction with HepG2 cells. The biosensor demonstrates a broad linear range of 50–10⁵ cells/mL with LOD of 26 cells/mL. Electrochemical surface area (ECSA) and optimization studies verify the stable sensor performance across different scan rates and pH conditions. It also exhibits selectivity, reproducibility, and reusability while retaining 85 % original signal after multiple regeneration cycles. Overall, PBA@GO-SiO₂ based biosensor is a scalable platform requiring small blood sample, eliminating the need for antibodies, ensuring long shelf life because of durable material, and carry translational potential at clinical level.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"168 ","pages":"Article 109098"},"PeriodicalIF":4.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrative and efficient microbiosensor for β-amyloid42 based on a molecularly imprinted layer coordinating built-in hemin on the acupuncture needle","authors":"Xue Kong,&nbsp;Zimei Yu,&nbsp;Qinghua Sun,&nbsp;Yan Liu,&nbsp;Zheng-Zhi Yin","doi":"10.1016/j.bioelechem.2025.109095","DOIUrl":"10.1016/j.bioelechem.2025.109095","url":null,"abstract":"<div><div>Monitoring beta-amyloid₁_–₄₂ (Aβ₄₂) is vital and challenging, which is a typical biomarker of Alzheimer's disease. Here, a novel electrochemical microbiosensor is developed to detect Aβ₄₂ on an acupuncture needle. Hemin is well known for its characteristics, including its ability to self-assemble on single-walled carbon nanotube (SWCNT), the molecular interaction with Aβ₄₂, and the intrinsic electroactive signal. These properties are exploited to anchor and respond to Aβ₄₂ after integrating a molecularly imprinted surface polymer (SMIP). The SMIP layer of polydopamine/poly (ionic liquid) can be prepared by electropolymerization on an acupuncture needle microelectrode (ANME), which undergoes growth and formation of a polymeric structure around the anchored Aβ₄₂. Interestingly, the imprinted cavities express a fluent signal of built-in hemin after eluting the templates, and show a highly selective and sensitive hindrance response for the recombined Aβ₄₂. Under optimized conditions, the microbiosensor displays a linear range of 100 to 1 × 10¹⁰ fM with a limit of detection of 0.05 fM. There are development and advances for the discipline of electroanalysis after comparing the technique and important indicators with the electrochemical biosensors reported of Aβ₄₂. The microbiosensor also exhibited excellent selectivity, good stability, and reproducibility, which was effectively used to detect Aβ₄₂ in real spiked samples. The improved behavior of the developed microbiosensor can be attributed to its superficial highly matched imprinted cavities, built-in hemin label, and electronic barrier without signal of the nonimprinted surface to outside molecules. This microbiosensor has a scientific and reference value for directly sensing non-electroactive biomarkers, functionalizing microelectrodes, and electron transport cavities. It would also be amazing if this new microbiosensor could combine with the unclear and magical property of acupuncture in the treatment of neurological disorders.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"Article 109095"},"PeriodicalIF":4.5,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial under-deposit corrosion of pipeline steel in alkali/surfactant/polymer solution","authors":"Victor Malachy Udowo ,&nbsp;Maocheng Yan ,&nbsp;Fuchun Liu ,&nbsp;Peter C. Okafor ,&nbsp;Alexander I. Ikeuba","doi":"10.1016/j.bioelechem.2025.109097","DOIUrl":"10.1016/j.bioelechem.2025.109097","url":null,"abstract":"<div><div>Alkali surfactant polymer (ASP) flooding technique is deployed in the oil industry to enhance oil recovery (EOR), especially in aged reservoirs. Microorganisms have been found to utilize the polymer constituent as a nutrient source to accelerate steel corrosion. This work aimed at examining the contributions of microorganisms, sediments, and polymer degradation to the overall corrosion behavior of steel pipes used in ASP flooding system via electrochemical, microscopic, and spectroscopic characterization techniques. The results demonstrate that rust deposition significantly influenced the steel's electrochemical behavior in the SRB-containing ASP solution; driving internal polarization and potential difference with the bare metal thereby establishing a galvanic interaction to accelerate steel corrosion. After 14 days of testing in the SRB environment, the steel covered by rust deposits recorded the most severe localized corrosion of all samples under examination, whereas pitting severity significantly reduced on the bare steel and the sand-deposited coupon. Factors such as sediment conductivity, film development, and microbial activities played crucial role in exacerbating steel corrosion under the rust deposits in the SRB-inoculated ASP solution.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"167 ","pages":"Article 109097"},"PeriodicalIF":4.5,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}