Bioelectrochemistry最新文献_第3页

Redox potential shapes spatial heterogeneity of mixed-cultured electroactive biofilm treating wastewater 氧化还原电位塑造了处理废水的混合培养电活性生物膜的空间异质性。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-10-15 DOI: 10.1016/j.bioelechem.2024.108836

Xian Wu , Guiqin Yang , Junhui Guo , Li Zhuang

{"title":"Redox potential shapes spatial heterogeneity of mixed-cultured electroactive biofilm treating wastewater","authors":"Xian Wu , Guiqin Yang , Junhui Guo , Li Zhuang","doi":"10.1016/j.bioelechem.2024.108836","DOIUrl":"10.1016/j.bioelechem.2024.108836","url":null,"abstract":"<div><div>The core of bioelectrochemical systems (BESs) is electrochemically active microorganisms (EAMs), which exert spatial heterogeneity on electrode surface and influences BESs performance. Setting an optimal potential is an effective strategy for improving and optimizing BESs performance, however, how the electrode potential affects spatial structure of microbial community within anode biofilm is not known. Using a complex substrate-fed BES with a wastewater inoculum, this study investigated the community structure and composition of the stratified biofilm developed under the potential of −0.3 V, 0 V, +0.3 V and +0.6 V (vs. saturated calomel electrode) by freezing microtome method and high-throughput sequencing analysis. The spatial heterogeneity of biofilm community was found to be dependent on the electrode potential and a less stratified community structure was observed for +0.6 V than other potentials. Within the biofilms, the inner layers selected more <em>Geobacter</em> and the outer layers enriched more <em>Acinetobacter</em> and <em>Serratia</em>, potentially suggested a stratification of electron transfer pathway and metabolite-based interspecies communications. The results demonstrated the response of spatial heterogeneity of anode biofilm community to the change of electrode potential, which helps to understand the selectivity and enrichment of kinetically efficient anodic microbiome by electron potential.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108836"},"PeriodicalIF":4.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Gram-positive and Gram-negative bacteria membrane permeabilization induced by pulsed electric field using electrochemical admittance spectroscopy 利用电化学导纳光谱检测脉冲电场诱导的革兰氏阳性菌和革兰氏阴性菌膜渗透。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-10-11 DOI: 10.1016/j.bioelechem.2024.108835

Mindaugas Visockis, Paulius Ruzgys, Simona Gelažunaitė, Salvijus Vykertas, Saulius Šatkauskas

{"title":"Detection of Gram-positive and Gram-negative bacteria membrane permeabilization induced by pulsed electric field using electrochemical admittance spectroscopy","authors":"Mindaugas Visockis, Paulius Ruzgys, Simona Gelažunaitė, Salvijus Vykertas, Saulius Šatkauskas","doi":"10.1016/j.bioelechem.2024.108835","DOIUrl":"10.1016/j.bioelechem.2024.108835","url":null,"abstract":"<div><div>Electrochemical impedance or admittance spectroscopy (EIS or EAS) has been widely used for decades, offering a label-free, rapid, real-time, and non-destructive assay for optically opaque and turbid bacterial solutions. However, PEF-induced changes in the bacterial envelope can present challenges in detecting the extent of membrane permeabilization in both Gram-positive and Gram-negative bacteria due to their distinct morphological properties. Here, we used a new approach for detecting bacterial membrane permeabilization induced by PEF using electrochemical admittance spectroscopy (EAS). The metabolic activity results have shown that the larger <em>L. d. bulgaricus</em> bacteria was found to be significantly more resistant to PEF strengths ranging from 4 to 16 kV/cm than the smaller <em>E. coli</em> bacteria at shorter PEF treatment durations (10 × 10 µs pulses). Interestingly, the difference in the increase of the admittance magnitude and a decrease in phase angle between the PEF treatment times of 10 × 10 µs and 10 × 100 µs pulses at different PEF strengths was more pronounced for <em>E. coli</em> bacteria samples. Our results demonstrate that EAS is more effective in comparing the degree of membrane permeabilization of Gram-positive and Gram-negative bacteria when longer PEF treatment durations are applied.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108835"},"PeriodicalIF":4.8,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical response mechanism of DNA damaged cells: DNA damage repair and purine metabolism activation DNA 损伤细胞的电化学反应机制：DNA 损伤修复和嘌呤代谢激活

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-10-09 DOI: 10.1016/j.bioelechem.2024.108832

Shulan Qi , Jiaqi Fu , Yue Li , Chaoqun Fei , Jiahuan Zhang , Liyuan Sui , Shi Zhou , Jinlian Li , Yanli Zhao , Dongmei Wu

{"title":"Electrochemical response mechanism of DNA damaged cells: DNA damage repair and purine metabolism activation","authors":"Shulan Qi , Jiaqi Fu , Yue Li , Chaoqun Fei , Jiahuan Zhang , Liyuan Sui , Shi Zhou , Jinlian Li , Yanli Zhao , Dongmei Wu","doi":"10.1016/j.bioelechem.2024.108832","DOIUrl":"10.1016/j.bioelechem.2024.108832","url":null,"abstract":"<div><div>In modern society, due to the sharp increase in pollutants that cause DNA damage, there is a growing demand for innovative detection techniques and biomarkers. In this paper, the electrochemical behavior of HepG2 cells exposed to CdCl<sub>2</sub> was investigated, and the electrochemical response mechanism of DNA damage was identified by exploring the correlation between the DNA damage response and purine metabolism. Western blot analysis revealed that the expression levels of ATM and Ku70 increased at 0.3 μM CdCl<sub>2</sub>, indicating a DNA damage response and activation of DNA repair processes. Simultaneously, elevated expression levels of PRPP aminotransferase, HPRT, and XOD were observed, leading to an increase in intracellular purine levels and electrochemical signals. The expression of Ku70 peaked at 0.5 μM CdCl<sub>2</sub>, indicating the highest DNA repair activity. The expression profiles of these purine metabolism proteins mirrored those of Ku70, suggesting a strong correlation between the activation of purine metabolism and DNA damage repair. Consistently, intracellular purine levels exhibited a similar trend, leading to corresponding changes in electrochemical signals. In summary, electrochemical using intracellular purines as biomarkers has the potential to emerge as a novel method for detecting early DNA damage.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108832"},"PeriodicalIF":4.8,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142419990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical/fluorescent dual-mode aptasensor based on 3D porous AuNPs/MXene for detection of ultra-trace mercury (Hg2+) 基于三维多孔 AuNPs/MXene 的电化学/荧光双模式灵敏传感器，用于检测超痕量汞 (Hg2+)。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-10-05 DOI: 10.1016/j.bioelechem.2024.108833

Luo Li , Xiaoyi Yan , Yu Liu , Yue Xing , Puyu Zhao , Yunxue Zhu , Ning Liu , Kaiping Sun , Zhiquan Zhang , Shengyong Zhai

{"title":"Electrochemical/fluorescent dual-mode aptasensor based on 3D porous AuNPs/MXene for detection of ultra-trace mercury (Hg2+)","authors":"Luo Li , Xiaoyi Yan , Yu Liu , Yue Xing , Puyu Zhao , Yunxue Zhu , Ning Liu , Kaiping Sun , Zhiquan Zhang , Shengyong Zhai","doi":"10.1016/j.bioelechem.2024.108833","DOIUrl":"10.1016/j.bioelechem.2024.108833","url":null,"abstract":"<div><div>In this work, the dual-mode aptasensor based on 3D porous AuNPs/MXene using “turn-on” electrochemical method and “turn-off” fluorescent strategy was fabricated. Here, 2D MXene was processed into 3D porous MXene by sacrificial polymethylmethacrylate (PMMA) spherical template. And the meteor hammer-like AuNPs which had good electrochemical properties and quenching effect on fluorescence was synthesized by single electrodeposition. Dual-signal labeled Nile Blue (NB) was in situ grafted to the Hg<sup>2+</sup> aptamer ends of 3D porous AuNPs/MXene/GCE, and an efficient and sensitive signal interface was constructed to realize the sensitive detection of Hg<sup>2+</sup>. 3D porous AuNPs/MXene had the advantages of large specific surface area, excellent electron transmission performance and signal amplification. The experimental results indicated that this sensor exhibited high sensitivity to Hg<sup>2+</sup> in both electrochemical and fluorescent sensing, with detection limits of 2.69 fM and 1.60 fM, respectively. Further, the dual-mode aptasensor can ensure the detection accuracy and target quantization. The dual-mode aptasensor has been successfully applied to the ultra-trace detection of Hg<sup>2+</sup> in actual water samples, which shows the potential of aptamer sensor in detecting heavy metal ions in environmental monitoring.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108833"},"PeriodicalIF":4.8,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitigation of biocorrosion of X80 carbon steel by a shale microbiome biofilm using a green biocide enhanced by d-amino acids 使用由 d-氨基酸增强的绿色杀菌剂，通过页岩微生物群生物膜缓解 X80 碳钢的生物腐蚀。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-30 DOI: 10.1016/j.bioelechem.2024.108831

Di Wang , Shengbei Wen , Hanjun Liu , Pan Liu , Jiahao Xiong , Yalin Wu , Zhilin Li , Zehong Tian , Bei Liu , Dake Xu , Tingyue Gu , Fuhui Wang

{"title":"Mitigation of biocorrosion of X80 carbon steel by a shale microbiome biofilm using a green biocide enhanced by d-amino acids","authors":"Di Wang , Shengbei Wen , Hanjun Liu , Pan Liu , Jiahao Xiong , Yalin Wu , Zhilin Li , Zehong Tian , Bei Liu , Dake Xu , Tingyue Gu , Fuhui Wang","doi":"10.1016/j.bioelechem.2024.108831","DOIUrl":"10.1016/j.bioelechem.2024.108831","url":null,"abstract":"<div><div>Microbiologically influenced corrosion (MIC) in shale gas field is a major threat with the hydraulic fracturing fluid injected into the subsurface. In this study, the microbiome collected from a shale gas produced water sample was extracted and cultivated in ATCC 1249 medium modified with 10 g/L NaCl anaerobically at 30 °C. <span>d</span>-amino acids, which were reported as biocide enhancers, were found to enhance 2,2-dibromo-3-nitrilopropionamide (DBNPA) biocide on the mitigation of shale microbiome MIC on X80 carbon steel. The combination of 50 ppm (w/w) <span>d</span>-leucine + 50 ppm <span>d</span>-alanine + 1 ppm <span>d</span>-tyrosine had the best enhancement effect on 50 ppm DBNPA with 84 % less weight loss, and 67 % lower corrosion current density (<em>i</em><sub>corr</sub>) compared to 50 ppm DBNPA alone. The corrosion data were consistent with the enhanced biofilm inhibition observation. The experimental data also indicated that <span>d</span>-tyrosine used alone at a low dosage of 1 ppm enhanced DBNPA considerably, with 44 % less weight loss and 47 % less <em>i</em><sub>corr</sub>. The electrochemical results showed the positive response of shale gas microbiome biofilm to the injected magnetite nanoparticles indicating the extracellular electron transfer might be a main mechanism for its corrosion.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108831"},"PeriodicalIF":4.8,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inside out: Exploring edible biocatalytic biosensors for health monitoring 由内而外：探索用于健康监测的可食用生物催化生物传感器。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-27 DOI: 10.1016/j.bioelechem.2024.108830

Verdiana Marchianò , Angelo Tricase , Alessandra Cimino , Blanca Cassano , Michele Catacchio , Eleonora Macchia , Luisa Torsi , Paolo Bollella

{"title":"Inside out: Exploring edible biocatalytic biosensors for health monitoring","authors":"Verdiana Marchianò , Angelo Tricase , Alessandra Cimino , Blanca Cassano , Michele Catacchio , Eleonora Macchia , Luisa Torsi , Paolo Bollella","doi":"10.1016/j.bioelechem.2024.108830","DOIUrl":"10.1016/j.bioelechem.2024.108830","url":null,"abstract":"<div><div>Edible biosensors can measure a wide range of physiological and biochemical parameters, including temperature, pH, gases, gastrointestinal biomarkers, enzymes, hormones, glucose, and drug levels, providing real-time data. Edible biocatalytic biosensors represent a new frontier within healthcare technology available for remote medical diagnosis. The main challenges to develop edible biosensors are: <em>i)</em> finding edible materials (i.e. redox mediators, conductive materials, binders and biorecognition elements such as enzymes) complying with Food and Drug Administration (FDA), European Food Safety Authority (EFSA) and European Medicines Agency (EMEA) regulations; ii) developing bioelectronics able to operate in extreme working conditions such as low pH (∼pH 1.5 gastric fluids etc.), body temperature (between 37 °C and 40 °C) and highly viscous bodily fluids that may cause surface biofouling issues. Nowadays, advanced printing techniques can revolutionize the design and manufacturing of edible biocatalytic biosensors.</div><div>This review outlines recent research on biomaterials suitable for creating edible biocatalytic biosensors, focusing on their electrochemical properties such as electrical conductivity and redox potential. It also examines biomaterials as substrates for printing and discusses various printing methods, highlighting challenges and perspectives for edible biocatalytic biosensors.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108830"},"PeriodicalIF":4.8,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bifunctional electrode materials: Enhancing microbial fuel cell efficiency with 3D hierarchical porous Fe3O4/Fe-N-C structures 双功能电极材料：利用三维分层多孔 Fe3O4/Fe-N-C 结构提高微生物燃料电池效率

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-22 DOI: 10.1016/j.bioelechem.2024.108829

Qiu-Ren Pan, Ying-Qi Ouyang, Hui-Huan Jiang, Dong-Ni Ou, Jun-Ying Zhong, Nan Li

{"title":"Bifunctional electrode materials: Enhancing microbial fuel cell efficiency with 3D hierarchical porous Fe3O4/Fe-N-C structures","authors":"Qiu-Ren Pan, Ying-Qi Ouyang, Hui-Huan Jiang, Dong-Ni Ou, Jun-Ying Zhong, Nan Li","doi":"10.1016/j.bioelechem.2024.108829","DOIUrl":"10.1016/j.bioelechem.2024.108829","url":null,"abstract":"<div><div>The rational development of high-performance anode and cathode electrodes for microbial fuel cells (MFCs) is crucial for enhancing MFC performance. However, complex synthesis methods and single-performance electrode materials hinder their large-scale implementation. Here, three-dimensional hierarchical porous (3DHP) Fe<sub>3</sub>O<sub>4</sub>/Fe-N-C composites were prepared via the hard template method. Notably, Fe<sub>3</sub>O<sub>4</sub>/Fe-N-C-0.04-600 demonstrated uniformly dispersed Fe<sub>3</sub>O<sub>4</sub> nanoparticles and abundant Fe-N<sub>x</sub> and pyridinic nitrogen, showing excellent catalytic performance for oxygen reduction reaction (ORR) with a half-wave potential (E<sub>1/2</sub>) of 0.74 V (vs. RHE), surpassing Pt/C (0.66 V vs. RHE). Moreover, Fe<sub>3</sub>O<sub>4</sub>/Fe-N-C-0.04-600 demonstrated favorable biocompatibility as an anode material, enhancing anode biomass and extracellular electron transfer efficiency. Sequencing results confirmed its promotion of electrophilic microorganisms in the anode biofilm. MFCs employing Fe<sub>3</sub>O<sub>4</sub>/Fe-N-C-0.04-600 as both anode and cathode materials achieved a maximum power density of 831.8 ± 27.7 mW m<sup>−2</sup>, enduring operation for 38 days. This study presents a novel approach for rational MFC design, emphasizing bifunctional materials capable of serving as anode materials for microorganism growth and as cathode catalysts for ORR catalysis.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108829"},"PeriodicalIF":4.8,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142319741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-energy electric shock ameliorates cell proliferation, morphallaxis, and regeneration via driving key regenerative proteins in earthworm and 3T3 cells 低能量电击通过驱动蚯蚓和 3T3 细胞中的关键再生蛋白，改善细胞增殖、变形和再生能力

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-21 DOI: 10.1016/j.bioelechem.2024.108824

Kamarajan Rajagopalan , Jackson Durairaj Selvan Christyraj , Nivetha Balamurugan , Johnson Retnaraj Samuel Selvan Christyraj , Vipin Mohan Dan , Periyasamy Radhakrishnan , Ashwin Barath Vaidhyalingham , Hari Prasath Nagaiah

{"title":"Low-energy electric shock ameliorates cell proliferation, morphallaxis, and regeneration via driving key regenerative proteins in earthworm and 3T3 cells","authors":"Kamarajan Rajagopalan , Jackson Durairaj Selvan Christyraj , Nivetha Balamurugan , Johnson Retnaraj Samuel Selvan Christyraj , Vipin Mohan Dan , Periyasamy Radhakrishnan , Ashwin Barath Vaidhyalingham , Hari Prasath Nagaiah","doi":"10.1016/j.bioelechem.2024.108824","DOIUrl":"10.1016/j.bioelechem.2024.108824","url":null,"abstract":"<div><div>Electric stimulation regulates many cellular processes like cell proliferation, differentiation, apoptosis and cellular migration. Despite its crucial role in regulating stem cells and regeneration, it remains underexplored in both in-vivo and in-vitro settings. In this study, <em>Eudrilus eugeniae</em> are subjected to electric stimulation (1.5 V) prior and after amputation and which augments regeneration up to double-time. Blocking epimorphosis using 2 M thymidine retracts regeneration kinetics to one-third but such inhibition was rescued by applying electric stimulation which propels an overactive morphallaxis pattern of regeneration. Excreting electric stimulation on control worms shows minimal impact, whereas it enhances the key regenerative proteins like VEGF, COX2, YAP, c-Myc, and Wnt3a on amputated worms. Upon blocking epimorphosis, all these key regenerative proteins are down-regulated but through electric stimulation, the cells are reprogrammed to express a triple fold of the mentioned regenerative proteins, that further promotes morphallaxis. In 3T3 cells, electric stimulation accelerates cell proliferation and migrations in 5 secs exposure and it exerts its function by overexpressing VEGF mediated by MEK1. Wnt3a expression was gradually upregulated in increasing exposure (5 and 25 secs) which aids in maintaining the stemness property. The molecular mechanism underlying regeneration capability can assist in designing novel therapeutic applications.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108824"},"PeriodicalIF":4.8,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142319742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A non-enzymatic hydrogen peroxide biosensor based on cerium metal-organic frameworks, hemin, and graphene oxide composite 基于铈金属有机框架、hemin 和氧化石墨烯复合材料的非酶过氧化氢生物传感器

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-21 DOI: 10.1016/j.bioelechem.2024.108823

Hamidreza Ghaedamini, Dong-Shik Kim

{"title":"A non-enzymatic hydrogen peroxide biosensor based on cerium metal-organic frameworks, hemin, and graphene oxide composite","authors":"Hamidreza Ghaedamini, Dong-Shik Kim","doi":"10.1016/j.bioelechem.2024.108823","DOIUrl":"10.1016/j.bioelechem.2024.108823","url":null,"abstract":"<div><div>This study presents the development of a novel non-enzymatic electrochemical biosensor for the real-time detection of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) based on a composite of cerium metal–organic frameworks (Ce-MOFs), hemin, and graphene oxide (GO). The Ce-MOFs served as an efficient matrix for hemin encapsulation, while GO enhanced the conductivity of the composite. Characterization techniques including scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, UV–vis spectroscopy, and thermogravimetric analysis (TGA) confirmed the successful integration of hemin into the Ce-MOFs. The Ce-MOFs@hemin/GO-modified sensor demonstrated sensitive H<sub>2</sub>O<sub>2</sub> detection due to the exceptional electrocatalytic activity of Ce-MOFs@hemin and the high conductivity of GO. This biosensor exhibited a linear response to H<sub>2</sub>O<sub>2</sub> concentrations from 0.05 to 10 mM with a limit of detection (LOD) of 9.3 μM. The capability of the biosensor to detect H<sub>2</sub>O<sub>2</sub> released from human prostate carcinoma cells was demonstrated, highlighting its potential for real-time monitoring of cellular oxidative stress in complex biological environments. To further assess its practical applicability, the sensor was tested in human serum samples, yielding promising results with recovery values ranging from 94.50 % to 103.29 %. In addition, the sensor showed excellent selectivity against common interfering compounds due to the outstanding peroxidase-like activity of the composite.</div></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"161 ","pages":"Article 108823"},"PeriodicalIF":4.8,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-mode detection of α-fetoprotein using the photothermal effect and peroxidase-like activity of Au@Cu/Cu2O-rGO 利用 Au@Cu/Cu2O-rGO 的光热效应和过氧化物酶样活性对α-胎儿蛋白进行双模式检测

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-09-21 DOI: 10.1016/j.bioelechem.2024.108822

Min Wang, Mingzhe Jiang, Wenjing Lai, Zihan Yan, Tingting Wang, Yu Qi, Chenglin Hong

引用次数: 0