Bioelectrochemistry最新文献_第3页

Leveraging self-signal amplifying poly(acrylic acid)/polyaniline electrodes for label-free electrochemical immunoassays in protein biomarker detection. 利用自信号放大聚（丙烯酸）/聚苯胺电极在蛋白质生物标志物检测中的无标记电化学免疫分析。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-12-28 DOI: 10.1016/j.bioelechem.2024.108894

Supakeit Chanarsa, Sopit Phetsang, Wiradej Thongsuwan, Thunwadee Limtharakul, Jidapha Tinoi, Jaroon Jakmunee, Kontad Ounnunkad

{"title":"Leveraging self-signal amplifying poly(acrylic acid)/polyaniline electrodes for label-free electrochemical immunoassays in protein biomarker detection.","authors":"Supakeit Chanarsa, Sopit Phetsang, Wiradej Thongsuwan, Thunwadee Limtharakul, Jidapha Tinoi, Jaroon Jakmunee, Kontad Ounnunkad","doi":"10.1016/j.bioelechem.2024.108894","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2024.108894","url":null,"abstract":"Accurate quantification of specific biomarkers is essential for clinical diagnosis and evaluating therapeutic efficacy. A self-signal-amplifying poly(acrylic acid) (PAA)/polyaniline (PANI) film-modified disposable and cost-effective screen-printed carbon electrode (SPCE) has been developed for constructing new label-free immunosensors targeting two model biomarkers: human immunoglobulin G (IgG) and alpha-fetoprotein (AFP). The electrochemically deposited PAA/PANI film on the SPCE serves a dual function: both a bio-immobilization support and a signal amplifier, enhancing biomarker detection sensitivity and efficiency. The self-signal amplification properties of PANI streamline the detection process. At the same time, the high-density surface carboxyl groups from embedded PAA enable covalent conjugation with capture antibodies (anti-IgG and anti-AFP). Subsequently, antibody-immobilized PAA/PANI film-modified SPCEs, as immunosensors, successfully detect IgG and AFP without the need for external redox probes. The reductions in the electrochemical PANI signals of the immunosensors are linearly proportional to the logarithm of IgG and AFP concentrations. The proposed immunosensors exhibit sufficiently wide ranges of calibration curves from 0.10 to 50 ng mL-1, with limits of detection of 0.080 ng mL-1 for IgG and 0.090 ng mL-1 for AFP. The sensors exhibit satisfactory sensitivity and selectivity, indicating their potential for accurate and reliable detection.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108894"},"PeriodicalIF":4.8,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrocultivation of Arabidopsis thaliana increases water and mineral absorption, electric charge and auxin accumulation, enhancing growth and development. 电栽培增加拟南芥对水分和矿物质的吸收、电荷和生长素的积累，促进生长发育。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-12-27 DOI: 10.1016/j.bioelechem.2024.108893

Sora Lee, Myung-Min Oh

{"title":"Electrocultivation of Arabidopsis thaliana increases water and mineral absorption, electric charge and auxin accumulation, enhancing growth and development.","authors":"Sora Lee, Myung-Min Oh","doi":"10.1016/j.bioelechem.2024.108893","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2024.108893","url":null,"abstract":"Numerous studies in various species have demonstrated that the application of an electric field can improve plant growth. However, plants showed inconsistent responses and the background mechanism for responses to electric fields remain unclear. Here, to deepen our understanding of the mechanisms involved in electric field-induced changes in physiology, we investigated the effects of electric fields on the growth and development of Arabidopsis (Arabidopsis thaliana). To this end, we cultivated Arabidopsis plants under 5 kV/m electric fields oriented vertically or horizontally. Regardless of the electric field direction, the exposed plants had significantly more leaves and greater biomass compared to the control group, which was not exposed to an additional electric field. Exposure to the electric fields also accelerated flowering. Auxin concentration in Arabidopsis leaves exhibited significant increase following exposure to electric field, supporting the enhanced shoot growth. Moreover, well-developed xylem and phloem under vertical electric fields facilitated increased absorption of water and nutrients. Gene ontology annotation and KEGG pathway analysis identified numerous electric field-responsive genes. Overall, this study elucidates mechanisms of the plant response to electric fields and represents a step towards developing technologies that enhance crop productivity.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108893"},"PeriodicalIF":4.8,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low background catalytic redox recycling coupled with hybridization chain reaction amplification for highly sensitive electrochemical aptamer luteinizing hormone assay. 低背景催化氧化还原循环耦合杂交链反应扩增高灵敏度电化学适体黄体生成素测定。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-12-26 DOI: 10.1016/j.bioelechem.2024.108888

Yuan Ming, Yujie Liu, Daxiu Li, Bingying Jiang, Yun Xiang, Ruo Yuan

{"title":"Low background catalytic redox recycling coupled with hybridization chain reaction amplification for highly sensitive electrochemical aptamer luteinizing hormone assay.","authors":"Yuan Ming, Yujie Liu, Daxiu Li, Bingying Jiang, Yun Xiang, Ruo Yuan","doi":"10.1016/j.bioelechem.2024.108888","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2024.108888","url":null,"abstract":"The concentration variation of luteinizing hormone (LH) regulates the cell cycle of oocyte meiosis and significantly affect the whole reproductive cycle. Sensitively quantifying the LH biomarker therefore plays an important role for reproductive disease diagnosis. By coupling a new low background catalytic redox recycling strategy with hybridization chain reaction (HCR), we propose a highly sensitive bio-electrochemical aptamer LH sensing method. LH analyte molecules bind aptamer strands in duplex DNAs to liberate ssDNAs, which trigger HCR generation of [Ru(NH3)6]Cl3 (RuHex)-modified dsDNA polymers on sensor electrode. Subsequent electrochemical redox recycling of RuHex mediated by K3[Fe(CN)6] thus exhibits greatly magnified currents for ultrasensitive LH assay. The synergistic integration of HCR signal amplification with low background redox recycling leads to highly enhanced signal-to-noise ratio and sensitivity for detecting LH down to 6.03 pM. In addition, LH sensing in diluted human serums has been tested and verified, making such sensor a robust detection platform for monitoring diverse biomarkers at low levels for early diagnosing diseases.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108888"},"PeriodicalIF":4.8,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A label-free electrochemical biosensor for sensitive analysis of the PARP-1 activity. 用于PARP-1活性敏感分析的无标记电化学生物传感器。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-12-26 DOI: 10.1016/j.bioelechem.2024.108891

Haitang Yang, Siming Pu, Penghua Shu, Jiapan Wang, YuYu Chen, Xinshuo Yang, Yuce Hou, Wei Wei

引用次数: 0

Sulfate reducing bacteria corrosion of a 90/10 Cu-Ni alloy coupled to an Al sacrificial anode. 90/10 Cu-Ni合金与Al牺牲阳极的硫酸盐还原细菌腐蚀。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-12-26 DOI: 10.1016/j.bioelechem.2024.108892

Huixuan Qian, Tianguan Wang, Peng Xu, Zhiyuan Feng, Bing Lei, Ping Zhang, Honglei Guo, Guozhe Meng

引用次数: 0

Efficient nitrate removal via microorganism-iron oxide co-evolution on biocathode surface. 生物阴极表面微生物-氧化铁协同进化高效去除硝酸盐。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-12-25 DOI: 10.1016/j.bioelechem.2024.108889

Xiaojun Liu, Huihui Dong, Qinyu Wang, Jing Yang, Xinru Zhai, Mingzhen Lin, Kaixin Liu, Qinzheng Yang

{"title":"Efficient nitrate removal via microorganism-iron oxide co-evolution on biocathode surface.","authors":"Xiaojun Liu, Huihui Dong, Qinyu Wang, Jing Yang, Xinru Zhai, Mingzhen Lin, Kaixin Liu, Qinzheng Yang","doi":"10.1016/j.bioelechem.2024.108889","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2024.108889","url":null,"abstract":"Sediment microbial fuel cell (SMFC) is a device for biological denitrification, in which electrons produced by sediment microorganisms can be transferred to the upper layer of the water column lacking electron donors. However, the low efficiency of denitrifying bacteria in acquiring electrons and enriching at the cathode greatly hinders the application of SMFC for nitrogen removal. In this study, we report a novel method of constructing a high-performance biocathode by modifying electrodes with zero-valent iron to enhance the enrichment and electron transfer of electroactive bacteria. The surface chemical and biological analysis of the biocathode revealed that the ZVI gradually oxidized to form magnetite and goethite, and finally stabilized into better crystallized lepidocrocite. On the other hand, the microbial community of the biocathode gradually evolved into a community dominated by denitrifying bacteria, specifically Clostridium. The co-evolved \"Clostridium-lepidocrocite\" composite endows the sediment microbial fuel cell with a 99% nitrate removal capacity. These results indicate that the cathode constructed by using ZVI modified electrode achieves efficient nitrate reduction by denitrifying bacteria. Furthermore, the construction method of biocathode may also have the potential application in water remediation and the geochemical cycling of elements.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108889"},"PeriodicalIF":4.8,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrical characteristics extraction and analysis method for the membrane of medaka embryo during its development using electrical impedance spectroscopy. 建立了一种利用电阻抗谱法提取和分析藻胚发育过程中膜电特性的方法。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-12-24 DOI: 10.1016/j.bioelechem.2024.108885

Lu Yang, Yang Wu, Songpei Hu, Jiafeng Yao, Fangming Chen

{"title":"An electrical characteristics extraction and analysis method for the membrane of medaka embryo during its development using electrical impedance spectroscopy.","authors":"Lu Yang, Yang Wu, Songpei Hu, Jiafeng Yao, Fangming Chen","doi":"10.1016/j.bioelechem.2024.108885","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2024.108885","url":null,"abstract":"An electrical characteristics extraction and analysis method for membrane of medaka embryo during its development using Electrical Impedance Spectroscopy (EIS) is proposed. The proposed method is non-invasive, it doesn't affect the embryo's development. Embryo's equivalent electrical circuit (EEC) model is established to extract membrane's electrical characteristics, it is used to fit the embryo's electrical impedance curves in different developmental stages. The equivalent resistances and capacitances of membrane are regarded as the electrical characteristics to analyze the membrane's physiological changes during embryo's development. To achieve the embryo's electrical impedance curve from the measurement system exactly, an EIT-assisted electrical impedance curve extraction method is innovatively introduced. The extracted electrical characteristics of the membrane reflect its physiological changes well in both simulation and experiment. In experiment, the equivalent capacitances of embryo's membrane are increasing, while the equivalent resistances show a downward trend from neurula stage to hatching stage. The experiment results indicate that the permeability of membrane becomes higher and the thickness of membrane becomes thinner from neurula stage to hatching stage.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108885"},"PeriodicalIF":4.8,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of bismuth tungstate perovskite photoelectrical performance using elemental co-doping and construction of ternary heterojunction for sensitive detection of Trenbolone. 元素共掺杂增强钨酸铋钙钛矿光电性能及构建三元异质结用于灵敏检测Trenbolone。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-12-19 DOI: 10.1016/j.bioelechem.2024.108887

Yanan Wu, Weixuan Xu, Feng Jiang, Shanghua Liu, Yueyuan Li, Shujun Wang, Zhen Xu, Yueyun Li

{"title":"Enhancement of bismuth tungstate perovskite photoelectrical performance using elemental co-doping and construction of ternary heterojunction for sensitive detection of Trenbolone.","authors":"Yanan Wu, Weixuan Xu, Feng Jiang, Shanghua Liu, Yueyuan Li, Shujun Wang, Zhen Xu, Yueyun Li","doi":"10.1016/j.bioelechem.2024.108887","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2024.108887","url":null,"abstract":"Bismuth tungstate perovskite has been identified as a promising photoelectric material. Nevertheless, the wide band gap of bismuth tungstate leads to short-wavelength absorption of a single material with an attenuated photocurrent response, hindering its realization in biosensing applications. In this study, F, S co-doped Bi2WO6 was synthesized by heat treatment and combined with SnS2 and CdS to form a ternary heterojunction composite. The resulting composite material, marked as F, S-Bi2WO6@SnS2@CdS, has excellent photoelectric characteristics. F, S co-doping can increase the number of oxygen vacancies, effectively reducing the band gap, and the introduction of narrow band gap metal-sulfur compounds can form ternary heterojunctions with them, further red-shifting the optical absorption wavelength, while greatly improving the photocurrent response through good energy level matching. The excellent level matching between AgInS2 and F,S-Bi2WO6@SnS2@CdS results in photocurrent enhancement. The competition between AgInS2-Ab-TB and AgInS2-Ab for limited binding sites leads to changes in the photocurrent signal, which can sensibly detect TB. The prepared PEC biosensor has excellent photocurrent response in the range of 0.1 pg/mL - 100 ng/mL, and the detection limit is 28.9 fg/mL. This study broadens the application of bismuth tungstate chalcogenide in biosensing and provides new ideas for the modification of other optoelectronic materials.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108887"},"PeriodicalIF":4.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel gold nanoparticles-Schiff base electrochemical sensor for the determination of lead (II) ions in biological samples. 新型金纳米粒子-希夫碱电化学传感器测定生物样品中铅离子。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-12-19 DOI: 10.1016/j.bioelechem.2024.108886

Zahra Akbari, Khouloud Abid, Daniela Iannazzo, Morteza Montazerozohori, Giovanni Neri

{"title":"Novel gold nanoparticles-Schiff base electrochemical sensor for the determination of lead (II) ions in biological samples.","authors":"Zahra Akbari, Khouloud Abid, Daniela Iannazzo, Morteza Montazerozohori, Giovanni Neri","doi":"10.1016/j.bioelechem.2024.108886","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2024.108886","url":null,"abstract":"In this investigation, a novel tetradentate Schiff base ligand, (ligand L) was synthesized using a simple chemical route assisted by triethylenetetramine with 4-dimethylaminocinnamaldehyde in ethanol. The chemical structure of the as-synthesized ligand was characterized using nuclear magnetic resonance (NMR) and UV-visible spectroscopy. This ligand was then employed to modify the working electrode of screen-printed carbon electrode (SPCE) for developing a modified L/SPCE sensor finalized to detection of lead ions (Pb2+). The electrochemical characteristics of the sensor were assessed by Square Wave Anodic Stripping Voltammetry technique (SWASV). To further enhance the sensitivity, gold nanoparticles (AuNps) were deposited on the surface of the working electrode for obtaining an AuNps-L/SPCE sensor. This device shows a linear response to Pb2+ until to 0.6 µM, a sensitivity of 897 µA µM-1 cm-2 and a limit of detection (LOD) of 0.38 μM. This successful strategy offers promising avenues for lead ion detection also in urine.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108886"},"PeriodicalIF":4.8,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly sensitive electrochemical Osteoprotegerin (OPG) immunosensor for assessing fracture healing and evaluating drug efficacy. 用于评估骨折愈合和药物疗效的高灵敏度电化学骨保护素（OPG）免疫传感器。

IF 4.8 2区化学

Bioelectrochemistry Pub Date : 2024-12-11 DOI: 10.1016/j.bioelechem.2024.108884

Lu Wang, Yan Pang, Zhipeng Zhang, Sichao Li, Nicole Jaffrezic-Renault, Kui Liu, Zhenzhong Guo

{"title":"Highly sensitive electrochemical Osteoprotegerin (OPG) immunosensor for assessing fracture healing and evaluating drug efficacy.","authors":"Lu Wang, Yan Pang, Zhipeng Zhang, Sichao Li, Nicole Jaffrezic-Renault, Kui Liu, Zhenzhong Guo","doi":"10.1016/j.bioelechem.2024.108884","DOIUrl":"https://doi.org/10.1016/j.bioelechem.2024.108884","url":null,"abstract":"Tibial fractures are common long bone injuries requiring effective monitoring for optimal healing. Osteoprotegerin (OPG), as a key marker of bone formation, is closely related to the degree of fracture healing. However, existing detection methods have certain limitations in sensitivity and specificity. This study successfully crafted an exceptionally sensitive electrochemical immunosensor based on COOH-CNFs/Ti3C2Tx MXene/PANI-AgNPs nanocomposite material for the quantitative analysis of OPG in serum, providing a methodological basis for auxiliary diagnosis of fracture healing degree and evaluation of drug efficacy. A one-pot hydrothermal method was employed to synthesize and modify the nanocomposite material on gold electrode surfaces, which exhibit high electrochemical activity, low charge transfer resistance, and a large electroactive surface area, thereby enhancing the immunosensor's conductivity and stability, with a wide linear range (10-17 to 10-12 g/mL) and a low detection limit (1.94 × 10-18 g/mL). Methodological validation further confirmed the immunosensor's excellent performance in specificity, reproducibility, and stability. Moreover, the successful application of this immunosensor in detecting OPG in serum samples from actual tibial fracture patients before and after medication demonstrates significant potential for clinical application in assisting the assessment of fracture healing and evaluating the efficacy of orthopedic drugs.","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"163 ","pages":"108884"},"PeriodicalIF":4.8,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0